RchiOBHm_Chr3g0455131

C4-type zinc-finger of DNA polymerase delta

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004063
Physical Location & Seq
Forward (+)
4984200 .. 4995066
10867 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42210

Sequence Viewer

Length: 5889 bp
ATGCCGCCTTCCGACGGCTTCAGCGTCCGGATCGTCTCCGTCGACTTCTACATGGCGCCGCCCATCCCTGGCCTCGATATCTCCTACAGTAGCTTCCACGGTGGGAAGGTGAATGAGGTACCTGTAATAAGAATTTACGGCTCTACTCCGGCCGGTCAGAAAGCTTGTCTGCACATTCACAGAGCATTACCATACCTATTTGTGCCGTGCAAAGATATACCGAATCAACCACATCAGAAAGGTGATTCGTACACATATGATATATCTCTTGCATTAGAGAAAGCTTTGAAGCTCAAAAGCAGTGCTGGTTCAAAGCGACAACATGTACATGGTTGCAGCCTTGTCCACGCAAGGAAGCTTTACGGTTACCATTCATCAGAGGAGGTGTTTGTGAAGATATACCTCTATTATCCACATGATGTGACTCGCGCCGCCAATCTTCTTCTGGGAGGTGCAGTTCTTGATAAATGCTTACAGCCCCACGAATCACACATTCCCTTCATTCTCCAATTTATGATTGACTACAACTTGTATGGGATGGGTCATCTGCATCTATCAAAACTGAAGTTTCGTCATCCAATGCCAGATGCTTTTACCCCTAGGAGATCTTTTTACAATGGACTGCCCGGTCAGGACATAGATAATTTAACTGGGATGTCTACAGATGTCCAGGCAGATATTAGTGACCATCTGCTTTTTGGTTCACCGGTTTGGATATCGTCCACAATTCCAGTAGACTGGACATGGCTTTCTTCTGGTGATGTTGAGGCTTCATCAAATAGAGATGTTAACTGCACTAAGCGACAAAGTATTTGTGAACTCGAGGGGGATGCTACTGTAGATGAGATACTCAATCAGCAGTTCAAAATGTATACATCCCTCTCGCAAACTCGTTCTGACGTGAAAATGGTTCAATCGCTTATACCAATTTGGGAGGAGTTTGAGAGAAATGGGATACACGAGGCAGCAATATCTTCTAATCCTGCTAAACCACTTCCAGAAGATGTTTTGAAAACTTTATCACTTGGTCCAGAATTTAATGAGAAACTTGTCAAATTGCATTTTGATACAGAAAACTCTTTGCGTTTAACTCAATTAAAGAAAGATGTAAAGTCTATGCACCAAATGACCTCTCCAAAAGATGGAGGAAATTCTGTTGGACTTCGAACTGATAATCTCAATTATGGAGAGATGATGGGATCTCTGTTGACACAAGGTTCTACTGAAGAGACGGATGCAAAACTGAATAAAGAACTGCCAGCATCTCAAGCGATTCAGACATTAAGTATGAAGGCTGCAGATAATGAAGCACTAGCTCTGCTTAGATGGCTTGCCACTTCTCAGGCAGCTGATGATTTGAACTCCGATGATGAACTTGTTCAGGAGACAATACTGAGTCCTTTGTTGCCTGCAACAACTATTGACAAAGTTCTGGAGAAAGCTAATATGGATTATGAGAGTGAATCCCAAAAGGAATGTCAGGACATCCTTGATTCGGTTGGTGACTTTGAGGGATCAAAGGAAAAAGATTCTTATCCCACTGATCGGAACTGTTCTTCCAAAGTTGAACCTGCATGTATGATTCTCCAGGTGAATGGTACCGGGGATCATGTTCTCTCAACTCCATCTCCTTGTGCCGGATCAATCGAAAATTTTTCTGAAGTAGAGATGAAGAATGAACTTAGGACTCCTGAACCCCAGGTTACGAAAGATGGTCTAAGCTCCAATCATAAACACAAAAGGAAAAAATTATTATGGGGCTCTTTACCTTTGTCTGCGGCCCAAAAGCTGAAGACTGAGAGTGAACCTGTCAGTTTGTACAAGGAAACTAAGGATCCTGTTGGTACAAGTGCCTCCTCTGAAAGTGAAGTGAGAAAACATGCAGGCACTGATGCTTGTGATAGTACGTTGGGAAGATGCTCTCTGCGGGACTTGATGAGGAGAAAGCGATCTTATCGAATTGAGCCACCTGAAAAAGAAGATACACTTTATCAAATGTTGCATTTTGACGAACTTAATGGGAAGTCTCGTGATTCTTTAGCTGACAGGCTATCATTAGCCCGACACTCAGTAAGTCATGAGGCTGATGATGTTAAAATAACCAATTCTGGCTGTTCCTTGTATGGTAAACTTCCACTTTTGTCTGGCAGTGTCAACTCTTTAAATGCAAGTACGTTAGATGGAAAATTTGACACAAATATCAAAATTGCTGCGGAAGTTGGCACTGGAGCAATTGCTCAACATGGGAACATCTTCACAGGCTCTTCTGAAGCTCCACCTGTGAATACAGGATTCTGGCAATTAAAGCCTGACAAGCTCGAGTCTGAAGCTTCCCTTGGCTGTTGTGAAACTTTCAACAAATCTAATGCTCAGACTTCCAAGTCAGGTGCTGAGGAAAACTTTTTTGTTGATGAGAGTCTTCATAGTTCTGCTGCTGACCCTGAAGCTTCTTGTGGAGATGGTCACACTGGAAGGGGCATTGATGGCTTATCTTCCAGAAGTGGAGTATCCGAGATACCTTTTGAGATTGGCACAAATGTCACAACTGACAAAACTGCTTTAAACAATGAAAGGTGTGGTAGCCAGAAACTGGGGAGAGATTCCTTTTTATCTGGGCTTAGTAACTTGTTGCCCTCGATCATCCAGTCTGATGATAAGGACTTAATTGGCATGACCTTCTCTAGGAAACCCCCAATTGCAGACTGGAAGGATGGGGGATTTCTGAGTTCTTCATATTTACAAGAGGAAGGAGCTTTGGATGATATTCTCCCATTTTTTGTGAGAGACTGCCAAAATGAGACAGACATCGAGAACAACTGTGTTAGAAATAGTGAGTCTCATTCTCATCAAGAATCTGCCTTAGGTGTCCCTACTCACTATCAAGCTGATGGCTCTTGTTTGTATCTGTTAACACCTGCAATTTCACCTCCTTCTGCCAAAAATGTATACCGCTGGCTGTCAAGAGGTGACAAAGGATCTTCTGCGAGTTTGACTGGTTCACAGAATTCTTTGCCTAATGATTTGAACGAGCCTTCACTCAAGTATGATTCTGTATGTGATGTGCAACCTAATCTACCCCAAGGACCTCAAGTAGATCATAGAAATCTTGAAGCAGAAAGGATTCCTTTTTGTAATGACATGACAGAAGTATTGGAAAGAGAAGCCGATGTTATAAGAGTTAAAACATACCCTGACCGCTCACAAGATAGTTCTCAGATTTCAGGTCCAGATGGGAGATCAAAATCCACTCCGCTTAGTCAAACTGGATTTCGAGACCCGGCAAGTGTTGGCAGTGGCCAGCAGCTGACGTTGTTAAGTATAGAGGTTCAAGCAGAATCTAGAGGTGATCTTCGACCCGATCCTCAGTTTGATGCTATCAATATTGTATTGCTTGCTATTCAGAATGATTGTGACTCCATCGTTGAAGTTCATGTACTTATGCACAGCGATGCTGAAAATTGTCAGAGGAGTCATGACGGGATTTCTGACTGCAAGCTGTTTGTGTTTCATGAAGAGAAGTACTTATTTCATCATTTTATCAAGTTTGTATGCTCGTTGGACCCTGATATATTAATGGGTTGGGATATTCAAGGTGGTTCCCTTGGTTTTTTGGCTGAAAGGGCATCACATCTTGGTATAGGTTTACTCAACAAGATATCTAGAACACCATCTGAAGCCAAGATGGTGGCTGAGGATTCAGAAGTTCCGGAGAAAGTCTTACAAGATAACCTGGCTGCTGACTCAATTGTTGCTGATCCAGTTGTATTAGAAGATCCAATAATTGAGGATGAATGGGGACGGACTCATTCCAGCGGTGTCCATGTTGGTGGTAGAATTGTACTGAATGTCTGGCGGCTGATGCGCAGTGAAGTGAAGCTTAATATGTACACAGTTGAAGCTGTTTCTGAAGTTGTGTTGAGGCGGAAAGTCCCATATATTCCTAATAAACTACTCACAAAATGGTTTTTAAGTGGTCCTGGAAGAGCAAGATACCGATGTATCGAATATGTGAAGGAGCGAGCAGAATTAAACCTTGAGATGATGAATCAATTAGATATGATAAATCGAACATCAGAACTTGCACGTGTCTTTGGGATTGACTTTTTTTCTGTTCTCTCTAGAGGCTCCCAATACCGTGTTGAATCTATGGTTCTGAGACTGGCACATACACAAAACTATCTTGCCATTTCCCCTGGGAAACAACAGGTAGCTTCGCAGCCAGCAATGGAATGCATCCCTCTTGTAATGGAACCAGAATCTGGTTTTTATGCAGATCCAGTAGTTGTTTTGGATTTTCAGTCTCTTTATCCATCAATGATAATTGCATACAACCTTTGCTATTCTACATGCCTTGGAAAAGTTGCGCCATCAGAGCCAAATACACTTGGTGTCAGTTCATTTTCACCAGATCCACATGATCTGCATGATTTAAAAGAGCAAATACTGCTTACTCCAAATGGTGTTATGTGTGTGCCAGCAAAGGTCCGTAAAGGCGTACTGCCACGCTTATTGGACGAAATATTGTCAACCAGAATAATGGTTAAACAAGCAATGAAAAAATTGTCTCCATCACAGAAAGTTCTTCACAGGATATTTAATGCAAGACAGCTTGCACTAAAGCTGATAGCAAACGTGACATATGGTTATACAGCTGCTGGATTTAGTGGCCGCATGCCTTGTGCAGAGATTGCAGACAGTGTTGTTCAGTGTGGCCGTAGCACACTTGAAAAGGCTATTTCATATGTAAATGCACATGACAGGTGGAAGGCTAGAGTTATTTATGGCGACACAGACAGCATGTTTGTCCTTCTAAAAGGCCGCACTAGGGAAGAGTCTTTTCAGATTGGACAGGAGATTGCATCTGAAATCAGTGCTATGAGTCCGACTCCAGTTGCTTTGAAGATGGAAAAAGTTTATCAGCCATGCTTCCTCCTCACCAAGAAGCGATATGTTGGTTACAGTTATGAAAGCCCTGGACAGAGTGAACCTATTTTTGATGCTAAAGGCATTGAGACAGTTCGCAGGGATACATGTGGGGCTGTTGCCAAGACAATGGAGCAGTCTCTGAGACTTTTTTTTGAACATCAAAACATGCCTGAGGTCAAAGCATACTTACAGCGCCAGTGGAAGCGAATTCTTTCTGGAAGAGTGTCCCTTCAGGATTTTGTATTTGCAAAAGAGGTTCGTCTAGGAACTTACCGTGCTAGTGGTTCATCACTTCCACCAGCTGCAATTGTTGCTACGAAAGCTATGAGAATTGACCCTAGGGCAGAACCACGTTATGCAGAGAGAGTACCTTACGTTGTAATACATGGGGAGCCTGGGGCCCGTCTGGCTGATGTGGTTGTGGATCCTTTGAACCTCCTGGCCACTGATTCACCCTATAGACTAAATGATATCTATTACATCCAAAAGCAGATAATCCCAGCTTTGCAGCGAGTGTTTGGGCTTGTTGGAGCTGACCTGCAGCAGTGGTTTTCAGATATGCCCCGCCCAGTAAGGGAAGCTTTTGGAAAACGCCCCTCTTATGCTTCAAATCCACACCGAACCAGAATTGACTATTACTATCTTTCAAGGCATTGCATATTGTGTGGTGAGTTGGTCCAGGGATCAGCCCATCTATGCAATCAATGTGCTGAAAACAAAACTGCTGCAGCAGCAGCTATAATCGGAAGAACTTCAAAGCTGGAGAGAGAGATGCAACACCTTGCTGCTATCTGTCGACATTGTGGAGGTGGGGACGCGCTTGAAGGAAATGGCATCAAATGCACTTCTCTTGCATGCTTAGTGTTTTATGAGAGACGCAAAGTTCAGAAAGAACTGCAGGGAACTGCTTCTGTTGCTGCAGAAACAGGTTTGTACCCTCAATGTTTGGTTGAGTGGTTTTGA

Protein Analysis

1962

Amino Acids

217.27

Weight (kDa)

5.81

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REV3_N PF24065 7 - 60 7.3e-18 DNA polymerase zeta catalytic subunit, N-terminal
POL3_N PF24055 62 - 141 2.2e-22 DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1 PF03104 1100 - 1225 1e-08 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 1368 - 1815 3.7e-100 DNA polymerase family B
zf-C4pol PF14260 1861 - 1934 6.4e-14 C4-type zinc-finger of DNA polymerase delta
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3173
AarI CACCTGC 1 cut(s) 2923
AasI GACNNNNNNGTC 2 cut(s) 627, 2380
Acc16I TGCGCA 1 cut(s) 3863
Acc36I ACCTGC 3 cut(s) 1579, 2923, 5472
Acc65I GGTACC 2 cut(s) 118, 1598
AccB1I GGYRCC 3 cut(s) 55, 118, 1598
AccB7I CCANNNNNTGG 3 cut(s) 1142, 2590, 4259
AccBSI CCGCTC 1 cut(s) 3198
AccI GTMKAC 6 cut(s) 42, 659, 735, 872, 2946, 5722
AccII CGCG 2 cut(s) 429, 5744
AccIII TCCGGA 2 cut(s) 27, 3706
AcoI YGGCCR 5 cut(s) 150, 3295, 4666, 4711, 5367
AcsI RAATTY 7 cut(s) 132, 1034, 1150, 1651, 2186, 3004, 5133
AcvI CACGTG 1 cut(s) 4085
AcyI GRCGYC 1 cut(s) 56
AdeI CACNNNGTG 1 cut(s) 4388
AflIII ACRYGT 3 cut(s) 322, 4084, 5030
AgeI ACCGGT 1 cut(s) 706
AjiI CACGTC 1 cut(s) 901
AjuI GAANNNNNNNTTGG 2 cut(s) 2319, 2351
AloI GAACNNNNNNTCC 4 cut(s) 441, 473, 1541, 1573
AlwNI CAGNNNCTG 7 cut(s) 1889, 2390, 2590, 3304, 4259, 4655, 5065
Ama87I CYCGRG 2 cut(s) 821, 2318
Aor13HI TCCGGA 2 cut(s) 27, 3706
AoxI GGCC 9 cut(s) 70, 150, 1779, 3295, 4666, 4711, 4816, 5325, 5367
ApaI GGGCCC 1 cut(s) 5329
ApoI RAATTY 7 cut(s) 132, 1034, 1150, 1651, 2186, 3004, 5133
ArsI GACNNNNNNTTYG 6 cut(s) 795, 827, 1462, 1494, 4784, 4816
AseI ATTAAT 1 cut(s) 3572
AsiGI ACCGGT 1 cut(s) 706
Asp700I GAANNNNTTC 6 cut(s) 1377, 2252, 3120, 5137, 5677, 5833
Asp718I GGTACC 2 cut(s) 118, 1598
AspA2I CCTAGG 2 cut(s) 599, 5264
AspLEI GCGC 6 cut(s) 58, 431, 3864, 4366, 5121, 5746
AsuC2I CCSGG 3 cut(s) 627, 1603, 3278
AsuII TTCGAA 1 cut(s) 1165
AvaI CYCGRG 2 cut(s) 821, 2318
AvaII GGWCC 7 cut(s) 1028, 3083, 3224, 3559, 3974, 4483, 5602
AvrII CCTAGG 2 cut(s) 599, 5264
AxyI CCTNAGG 2 cut(s) 2860, 5097
BaeGI GKGCMC 1 cut(s) 5329
BaeI ACNNNNGTAYC 4 cut(s) 3982, 3982, 4015, 4015
BalI TGGCCA 2 cut(s) 3297, 5369
BamHI GGATCC 2 cut(s) 1834, 5350
BanI GGYRCC 3 cut(s) 55, 118, 1598
BanII GRGCYC 2 cut(s) 1763, 5329
BarI GAAGNNNNNNTAC 2 cut(s) 3417, 3449
BauI CACGAG 2 cut(s) 959, 2028
BbrPI CACGTG 1 cut(s) 4085
BbsI GAAGAC 2 cut(s) 1799, 2411
BbvCI CCTCAGC 2 cut(s) 2391, 3690
BceAI ACGGC 4 cut(s) 31, 154, 190, 4698
BcgI CGANNNNNNTGC 2 cut(s) 812, 846
BciVI GTATCC 3 cut(s) 948, 2518, 5020
BcnI CCSGG 3 cut(s) 627, 1603, 3278
BfmI CTRYAG 9 cut(s) 85, 660, 837, 1296, 5383, 5465, 5652, 5822, 5844
BfoI RGCGCY 2 cut(s) 59, 5122
BfuAI ACCTGC 3 cut(s) 1579, 2923, 5472
BfuI GTATCC 3 cut(s) 948, 2518, 5020
BglI GCCNNNNNGGC 1 cut(s) 5333
BglII AGATCT 1 cut(s) 605
BlnI CCTAGG 2 cut(s) 599, 5264
BmcAI AGTACT 1 cut(s) 3521
Bme18I GGWCC 7 cut(s) 1028, 3083, 3224, 3559, 3974, 4483, 5602
BmeT110I CYCGRG 2 cut(s) 821, 2318
BmgBI CACGTC 1 cut(s) 901
BmrI ACTGGG 3 cut(s) 660, 2600, 5489
BmuI ACTGGG 3 cut(s) 660, 2600, 5489
BpiI GAAGAC 2 cut(s) 1799, 2411
BplI GAGNNNNNCTC 2 cut(s) 4870, 4902
BpmI CTGGAG 5 cut(s) 1454, 1571, 2247, 4872, 5708
Bpu10I CCTNAGC 2 cut(s) 2391, 3690
Bpu14I TTCGAA 1 cut(s) 1165
BpuEI CTTGAG 4 cut(s) 1251, 3023, 3072, 4055
BpuMI CCSGG 3 cut(s) 627, 1603, 3278
BsaAI YACGTR 1 cut(s) 4085
BsaBI GATNNNNATC 2 cut(s) 1533, 3241
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 3267
BsaWI WCCGGW 3 cut(s) 27, 706, 3706
BsaXI ACNNNNNCTCC 4 cut(s) 441, 471, 1612, 1642
Bse118I RCCGGY 2 cut(s) 152, 706
Bse21I CCTNAGG 2 cut(s) 2860, 5097
Bse3DI GCAATG 3 cut(s) 4230, 4557, 5578
Bse8I GATNNNNATC 2 cut(s) 1533, 3241
BseAI TCCGGA 2 cut(s) 27, 3706
BseJI GATNNNNATC 2 cut(s) 1533, 3241
BseMI GCAATG 3 cut(s) 4230, 4557, 5578
BseRI GAGGAG 6 cut(s) 395, 950, 1846, 1954, 3481, 4922
BseSI GKGCMC 1 cut(s) 5329
BseX3I CGGCCG 1 cut(s) 150
BseYI CCCAGC 1 cut(s) 5425
BsgI GTGCAG 4 cut(s) 155, 474, 778, 4701
Bsh1236I CGCG 2 cut(s) 429, 5744
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 9 cut(s) 72, 152, 1781, 3297, 4668, 4713, 4818, 5327, 5369
BshNI GGYRCC 3 cut(s) 55, 118, 1598
BshTI ACCGGT 1 cut(s) 706
BsiEI CGRYCG 1 cut(s) 153
BsiHKCI CYCGRG 2 cut(s) 821, 2318
BsiSI CCGG 9 cut(s) 28, 149, 153, 627, 707, 1602, 1638, 3278, 3707
BslFI GGGAC 6 cut(s) 1943, 2852, 3810, 3914, 5137, 5753
BsmBI CGTCTC 3 cut(s) 40, 1223, 5794
BsmFI GGGAC 6 cut(s) 1943, 2852, 3810, 3914, 5137, 5753
BsmI GAATGC 1 cut(s) 4235
BsnI GGCC 9 cut(s) 72, 152, 1781, 3297, 4668, 4713, 4818, 5327, 5369
Bso31I GGTCTC 1 cut(s) 3267
BsoBI CYCGRG 2 cut(s) 821, 2318
Bsp119I TTCGAA 1 cut(s) 1165
Bsp120I GGGCCC 1 cut(s) 5325
Bsp1286I GDGCHC 2 cut(s) 1763, 5329
Bsp13I TCCGGA 2 cut(s) 27, 3706
Bsp1407I TGTACA 3 cut(s) 325, 1818, 3885
BspANI GGCC 9 cut(s) 72, 152, 1781, 3297, 4668, 4713, 4818, 5327, 5369
BspEI TCCGGA 2 cut(s) 27, 3706
BspFNI CGCG 2 cut(s) 429, 5744
BspHI TCATGA 3 cut(s) 2077, 3472, 3508
BspMAI CTGCAG 5 cut(s) 1300, 5469, 5656, 5826, 5848
BspMI ACCTGC 3 cut(s) 1579, 2923, 5472
BspQI GCTCTTC 2 cut(s) 2269, 3976
BspT104I TTCGAA 1 cut(s) 1165
BspT107I GGYRCC 3 cut(s) 55, 118, 1598
BspTNI GGTCTC 1 cut(s) 3267
BsrBI CCGCTC 1 cut(s) 3198
BsrDI GCAATG 3 cut(s) 4230, 4557, 5578
BsrFI RCCGGY 2 cut(s) 152, 706
BsrGI TGTACA 3 cut(s) 325, 1818, 3885
BssAI RCCGGY 2 cut(s) 152, 706
BssNAI GTATAC 2 cut(s) 873, 2947
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 2 cut(s) 959, 2028
BssT1I CCWWGG 6 cut(s) 599, 2335, 3079, 3601, 4351, 5264
Bst1107I GTATAC 2 cut(s) 873, 2947
Bst2BI CACGAG 2 cut(s) 959, 2028
Bst6I CTCTTC 6 cut(s) 1221, 2269, 3507, 3976, 4824, 5140
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 4 cut(s) 4444, 4688, 5237, 5766
BstAUI TGTACA 3 cut(s) 325, 1818, 3885
BstBAI YACGTR 1 cut(s) 4085
BstBI TTCGAA 1 cut(s) 1165
BstDSI CCRYGG 1 cut(s) 97
BstEII GGTNACC 1 cut(s) 365
BstENI CCTNNNNNAGG 1 cut(s) 2680
BstFNI CGCG 2 cut(s) 429, 5744
BstH2I RGCGCY 2 cut(s) 59, 5122
BstHHI GCGC 6 cut(s) 58, 431, 3864, 4366, 5121, 5746
BstMCI CGRYCG 1 cut(s) 153
BstNSI RCATGY 9 cut(s) 326, 1578, 1883, 4350, 4675, 4801, 5034, 5095, 5784
BstPI GGTNACC 1 cut(s) 365
BstSFI CTRYAG 9 cut(s) 85, 660, 837, 1296, 5383, 5465, 5652, 5822, 5844
BstSLI GKGCMC 1 cut(s) 5329
BstUI CGCG 2 cut(s) 429, 5744
BstV2I GAAGAC 2 cut(s) 1799, 2411
BstX2I RGATCY 8 cut(s) 605, 1199, 1834, 2975, 3772, 4273, 4408, 5350
BstXI CCANNNNNNTGG 6 cut(s) 738, 1595, 3685, 3827, 4537, 5053
BstYI RGATCY 8 cut(s) 605, 1199, 1834, 2975, 3772, 4273, 4408, 5350
BstZ17I GTATAC 2 cut(s) 873, 2947
BstZI CGGCCG 1 cut(s) 150
Bsu36I CCTNAGG 2 cut(s) 2860, 5097
BsuI GTATCC 3 cut(s) 948, 2518, 5020
BsuRI GGCC 9 cut(s) 72, 152, 1781, 3297, 4668, 4713, 4818, 5327, 5369
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 3462
BtrI CACGTC 1 cut(s) 901
BtsI GCAGTG 5 cut(s) 307, 2155, 3298, 3871, 5477
BveI ACCTGC 3 cut(s) 1579, 2923, 5472
CaiI CAGNNNCTG 7 cut(s) 1889, 2390, 2590, 3304, 4259, 4655, 5065
CciI TCATGA 3 cut(s) 2077, 3472, 3508
CfoI GCGC 6 cut(s) 58, 431, 3864, 4366, 5121, 5746
Cfr10I RCCGGY 2 cut(s) 152, 706
CseI GACGC 3 cut(s) 13, 5750, 5811
CspAI ACCGGT 1 cut(s) 706
CspCI CAANNNNNGTGG 2 cut(s) 4491, 4526
DinI GGCGCC 1 cut(s) 57
DraI TTTAAA 3 cut(s) 2163, 2562, 4431
DraIII CACNNNGTG 1 cut(s) 4388
DrdI GACNNNNNNGTC 2 cut(s) 627, 2380
DseDI GACNNNNNNGTC 2 cut(s) 627, 2380
EaeI YGGCCR 5 cut(s) 150, 3295, 4666, 4711, 5367
EagI CGGCCG 1 cut(s) 150
Eam1104I CTCTTC 6 cut(s) 1221, 2269, 3507, 3976, 4824, 5140
EarI CTCTTC 6 cut(s) 1221, 2269, 3507, 3976, 4824, 5140
EciI GGCGGA 1 cut(s) 3937
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 6 cut(s) 599, 2335, 3079, 3601, 4351, 5264
Eco24I GRGCYC 2 cut(s) 1763, 5329
Eco31I GGTCTC 1 cut(s) 3267
Eco32I GATATC 4 cut(s) 79, 717, 3657, 5398
Eco47I GGWCC 7 cut(s) 1028, 3083, 3224, 3559, 3974, 4483, 5602
Eco52I CGGCCG 1 cut(s) 150
Eco72I CACGTG 1 cut(s) 4085
Eco81I CCTNAGG 2 cut(s) 2860, 5097
Eco88I CYCGRG 2 cut(s) 821, 2318
Eco91I GGTNACC 1 cut(s) 365
EcoNI CCTNNNNNAGG 1 cut(s) 2680
EcoO109I RGGNCCY 2 cut(s) 3083, 5325
EcoO65I GGTNACC 1 cut(s) 365
EcoRI GAATTC 2 cut(s) 3004, 5133
EcoRV GATATC 4 cut(s) 79, 717, 3657, 5398
EcoT14I CCWWGG 6 cut(s) 599, 2335, 3079, 3601, 4351, 5264
EcoT22I ATGCAT 1 cut(s) 4235
EcoT38I GRGCYC 2 cut(s) 1763, 5329
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 6 cut(s) 599, 2335, 3079, 3601, 4351, 5264
Esp3I CGTCTC 3 cut(s) 40, 1223, 5794
FaqI GGGAC 6 cut(s) 1943, 2852, 3810, 3914, 5137, 5753
FauI CCCGC 2 cut(s) 1920, 5498
FauNDI CATATG 3 cut(s) 256, 4639, 4741
FblI GTMKAC 6 cut(s) 42, 659, 735, 872, 2946, 5722
FriOI GRGCYC 2 cut(s) 1763, 5329
FspI TGCGCA 1 cut(s) 3863
GlaI GCGC 6 cut(s) 57, 430, 3863, 4365, 5120, 5745
GsaI CCCAGC 1 cut(s) 5429
GsuI CTGGAG 5 cut(s) 1454, 1571, 2247, 4872, 5708
HaeII RGCGCY 2 cut(s) 59, 5122
HaeIII GGCC 9 cut(s) 72, 152, 1781, 3297, 4668, 4713, 4818, 5327, 5369
HapII CCGG 9 cut(s) 28, 149, 153, 627, 707, 1602, 1638, 3278, 3707
HgaI GACGC 3 cut(s) 13, 5750, 5811
HhaI GCGC 6 cut(s) 58, 431, 3864, 4366, 5121, 5746
Hin1I GRCGYC 1 cut(s) 56
Hin6I GCGC 6 cut(s) 56, 429, 3862, 4364, 5119, 5744
HinP1I GCGC 6 cut(s) 56, 429, 3862, 4364, 5119, 5744
HincII GTYRAC 7 cut(s) 43, 790, 1209, 2155, 2910, 4527, 5723
HindII GTYRAC 7 cut(s) 43, 790, 1209, 2155, 2910, 4527, 5723
HindIII AAGCTT 7 cut(s) 162, 282, 356, 2328, 2445, 3875, 5505
HpaI GTTAAC 2 cut(s) 790, 2910
HpaII CCGG 9 cut(s) 28, 149, 153, 627, 707, 1602, 1638, 3278, 3707
Hpy99I CGWCG 2 cut(s) 17, 44
HpyCH4IV ACGT 8 cut(s) 900, 1907, 2174, 3308, 4084, 4632, 5278, 5301
HpySE526I ACGT 8 cut(s) 900, 1907, 2174, 3308, 4084, 4632, 5278, 5301
Hsp92I GRCGYC 1 cut(s) 56
HspAI GCGC 6 cut(s) 56, 429, 3862, 4364, 5119, 5744
KasI GGCGCC 1 cut(s) 55
Kpn2I TCCGGA 2 cut(s) 27, 3706
KpnI GGTACC 2 cut(s) 122, 1602
KspAI GTTAAC 2 cut(s) 790, 2910
LguI GCTCTTC 2 cut(s) 2269, 3976
LmnI GCTCC 9 cut(s) 1727, 2228, 2278, 2750, 4015, 4130, 5056, 5317, 5456
MaeII ACGT 8 cut(s) 900, 1907, 2174, 3308, 4084, 4632, 5278, 5301
MbiI CCGCTC 1 cut(s) 3198
MfeI CAATTG 4 cut(s) 2232, 2694, 3744, 5232
MflI RGATCY 8 cut(s) 605, 1199, 1834, 2975, 3772, 4273, 4408, 5350
MhlI GDGCHC 2 cut(s) 1763, 5329
MlsI TGGCCA 2 cut(s) 3297, 5369
MluNI TGGCCA 2 cut(s) 3297, 5369
Mly113I GGCGCC 1 cut(s) 56
MmeI TCCRAC 5 cut(s) 36, 1138, 3537, 4907, 5434
Mox20I TGGCCA 2 cut(s) 3297, 5369
Mph1103I ATGCAT 1 cut(s) 4235
MroI TCCGGA 2 cut(s) 27, 3706
MroXI GAANNNNTTC 6 cut(s) 1377, 2252, 3120, 5137, 5677, 5833
MscI TGGCCA 2 cut(s) 3297, 5369
MslI CAYNNNNRTG 3 cut(s) 327, 3447, 3825
Msp20I TGGCCA 2 cut(s) 3297, 5369
MspA1I CMGCKG 6 cut(s) 1349, 2952, 3304, 3813, 4652, 5228
MspI CCGG 9 cut(s) 28, 149, 153, 627, 707, 1602, 1638, 3278, 3707
MunI CAATTG 4 cut(s) 2232, 2694, 3744, 5232
Mva1269I GAATGC 1 cut(s) 4235
MvnI CGCG 2 cut(s) 429, 5744
NarI GGCGCC 1 cut(s) 56
NciI CCSGG 3 cut(s) 627, 1603, 3278
NdeI CATATG 3 cut(s) 256, 4639, 4741
NmuCI GTSAC 8 cut(s) 421, 683, 1502, 2462, 2539, 2966, 3410, 4633
NsbI TGCGCA 1 cut(s) 3863
NsiI ATGCAT 1 cut(s) 4235
NspI RCATGY 9 cut(s) 326, 1578, 1883, 4350, 4675, 4801, 5034, 5095, 5784
NspV TTCGAA 1 cut(s) 1165
PaeI GCATGC 2 cut(s) 4675, 5784
PaeR7I CTCGAG 2 cut(s) 821, 2318
PagI TCATGA 3 cut(s) 2077, 3472, 3508
PaqCI CACCTGC 1 cut(s) 2923
PasI CCCWGGG 1 cut(s) 4193
PciI ACATGT 2 cut(s) 322, 5030
PciSI GCTCTTC 2 cut(s) 2269, 3976
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 39
PctI GAATGC 1 cut(s) 4235
PdmI GAANNNNTTC 6 cut(s) 1377, 2252, 3120, 5137, 5677, 5833
PflMI CCANNNNNTGG 3 cut(s) 1142, 2590, 4259
PfoI TCCNGGA 1 cut(s) 3976
PinAI ACCGGT 1 cut(s) 706
PluTI GGCGCC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 4085
PmlI CACGTG 1 cut(s) 4085
Ppu21I YACGTR 1 cut(s) 4085
PpuMI RGGWCCY 1 cut(s) 3083
PscI ACATGT 2 cut(s) 322, 5030
PshBI ATTAAT 1 cut(s) 3572
PsiI TTATAA 1 cut(s) 3173
Psp5II RGGWCCY 1 cut(s) 3083
PspCI CACGTG 1 cut(s) 4085
PspEI GGTNACC 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 5425
PspOMI GGGCCC 1 cut(s) 5325
PspPPI RGGWCCY 1 cut(s) 3083
PspXI VCTCGAGB 2 cut(s) 821, 2318
PstI CTGCAG 5 cut(s) 1300, 5469, 5656, 5826, 5848
PstNI CAGNNNCTG 7 cut(s) 1889, 2390, 2590, 3304, 4259, 4655, 5065
PsuI RGATCY 8 cut(s) 605, 1199, 1834, 2975, 3772, 4273, 4408, 5350
PvuII CAGCTG 4 cut(s) 1349, 3304, 4652, 5228
RseI CAYNNNNRTG 3 cut(s) 327, 3447, 3825
SalI GTCGAC 2 cut(s) 41, 5721
SapI GCTCTTC 2 cut(s) 2269, 3976
ScaI AGTACT 1 cut(s) 3521
SduI GDGCHC 2 cut(s) 1763, 5329
SfcI CTRYAG 9 cut(s) 85, 660, 837, 1296, 5383, 5465, 5652, 5822, 5844
SfoI GGCGCC 1 cut(s) 57
Sfr274I CTCGAG 2 cut(s) 821, 2318
SfuI TTCGAA 1 cut(s) 1165
SinI GGWCC 7 cut(s) 1028, 3083, 3224, 3559, 3974, 4483, 5602
SlaI CTCGAG 2 cut(s) 821, 2318
SmiMI CAYNNNNRTG 3 cut(s) 327, 3447, 3825
SmlI CTYRAG 6 cut(s) 821, 1266, 2318, 3038, 3087, 4034
SmoI CTYRAG 6 cut(s) 821, 1266, 2318, 3038, 3087, 4034
SphI GCATGC 2 cut(s) 4675, 5784
SspDI GGCGCC 1 cut(s) 55
SspI AATATT 2 cut(s) 3382, 4521
StyI CCWWGG 6 cut(s) 599, 2335, 3079, 3601, 4351, 5264
TaiI ACGT 8 cut(s) 903, 1910, 2177, 3311, 4087, 4635, 5281, 5304
TatI WGTACW 6 cut(s) 325, 1818, 3433, 3519, 3838, 3885
TauI GCSGC 7 cut(s) 7, 61, 434, 1781, 3856, 4671, 4821
TseFI GTSAC 8 cut(s) 421, 683, 1502, 2462, 2539, 2966, 3410, 4633
Tsp45I GTSAC 8 cut(s) 421, 683, 1502, 2462, 2539, 2966, 3410, 4633
TspGWI ACGGA 4 cut(s) 28, 1247, 3814, 4475
Van91I CCANNNNNTGG 3 cut(s) 1142, 2590, 4259
VpaK11BI GGWCC 7 cut(s) 1028, 3083, 3224, 3559, 3974, 4483, 5602
VspI ATTAAT 1 cut(s) 3572
XagI CCTNNNNNAGG 1 cut(s) 2680
XapI RAATTY 7 cut(s) 132, 1034, 1150, 1651, 2186, 3004, 5133
XbaI TCTAGA 3 cut(s) 3338, 3659, 4118
XceI RCATGY 9 cut(s) 326, 1578, 1883, 4350, 4675, 4801, 5034, 5095, 5784
XcmI CCANNNNNNNNNTGG 3 cut(s) 442, 695, 2700
XhoI CTCGAG 2 cut(s) 821, 2318
XmaJI CCTAGG 2 cut(s) 599, 5264
XmiI GTMKAC 6 cut(s) 42, 659, 735, 872, 2946, 5722
XmnI GAANNNNTTC 6 cut(s) 1377, 2252, 3120, 5137, 5677, 5833
ZrmI AGTACT 1 cut(s) 3521
Zsp2I ATGCAT 1 cut(s) 4235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.