Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001413

Physical Location & Seq

Genomic Coordinates Reverse (-)

30717860 .. 30722280

Gene Structure 4421 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1232400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGGCGGTTCCACATTTGAGCATGTTGATCTCAAAGTTACAAAATCTATCCACTTCGGACCACTCGTCTGTGGTTTCGATGAACCTTTTCGTGGCACTACTTTTAGCTTGTATTGTGATCGGACATCTTCTCGAGGAGAATCGATGGGTGAATGAGTCGATCACCGCCCTTTTGATTGGTATATGTACTGGAGTAGTTATTCTTCTGATCAGTCGAGGAAAAAGTTCGCATCTTTTGGTTTTCAGTGAAGATCTTTTCTTTATATACCTCCTTCCGCCTATTATTTTTAATGCCGGGTTTCAGGTGAAAAAGAAGCAGTTCTTTGTTAACTTCAGCACCATTGTACTGTTTGGTGCCATTGGTACATTAGTATCCTGCACTATCATATCATTAGGCATGCTCGTAACTCTGAACATGCTGTTTCCCCGATGCGCTACACAATTCTTTAAGAAATTGGATATTGGAACTCTGGAATTGGGGGACTTTCTTGCGATTGGTGCAATATTTGCTGCAACGGATTCTGTTTGCACGTTGCAGGTGCTCAATCAAGATGAGACACCTTTACTCTACAGTCTTGTGTTCGGGGAGGGTGTCGTTAACGATGCGACATCTGTGGTTCTTTTCAATGCTATTCAGAGCTTTGATCTCACCCACCTTGATTCCGGCATTGCCTTGCACTTTCTGGGAAACTTCTTTTATTTGTTTTTCGCAAGCACCATGCTAGGAGTGTTTGCAGGGCTGCTTAGTGCTTACATTATAAAAAAACTTTATTTTGCAAGGCACTCTACGGATCGTGAGGTTGCTCTTATGATGCTCATGGCATACCTGTCATATATACTGGCTGAATTATTCTATTTGAGTGGCATTCTCACTGTGTTCTTTTGTGGGATCGTGATGTCGCATTACACTTGGCACAATGTGACTGAGAGTTCAAGAGTTACGACCAAGCATGCTTTCGCAACCTTGTCATTTGTTGCCGAAATATTTATCTTCCTTTATGTTGGTATGGATGCCTTGGACATTGAAAAGTGGAGATTTGTAAGCGACAGTCCTGGAACATCAGTGGCGGTGAGTTCAATACTGCTAGGTCTTCTTATGCTAGGAAGAGCAGCTTTTATTTTCCCCCTATCATTTTTGTCAAACTTAGCAAAGAAAAACCAACATGAGAAAATCAGCCTCCGGCAGCAAGTGATAATATGGTGGGCTGGTCTCATGAGAGGTGCTGTTTCGATAGCACTAGCTTACAATCAGTTTACGAGGTCAGGCCACACGCAGTTGCGAGCAAATGCAATCATGATCACTAGCACGATAACTGTTGTTCTTGTCAGCACAGTGGTGTTCGGATTGATGACAAAACCTCTTATAAGGTTCTTGCTGCCTCATTCATCAAAACAAACAACCAGCATGCTGTCATCAGAACCAACCACTCCAAAATCAATCATTATTCCACTTCTAGGGCAGGATTCTGTAGATGATCTCGTTATCCAAGATATTCGACGGCCAGCCAGCATTCGCGATCTTCTGACGACTCCATTTAATAGGCACACTGTCCATCGCTATTGGCGTAAGTTTGATAACGCCTTCATGCGACCGGTGTTTGGAGGCCGGGGTTTTGTTCCCTTTGTTCCCGGCTCACCAACTGAACGGAACAACAACGTTCAGTGGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006873 GO:0006885 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009628 GO:0009651 GO:0009987 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015078 GO:0015079 GO:0015081 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015385 GO:0015386 GO:0015491 GO:0015672 GO:0016020 GO:0019725 GO:0022804 GO:0022821 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030641 GO:0031090 GO:0032501 GO:0032502 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0090333 GO:0098588 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098719 GO:0098739 GO:0098771 GO:0098805 GO:0099402 GO:0099516 GO:0099587 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

557

Amino Acids

61.9

Weight (kDa)

8.92

Isoelectric Point (pI)

33.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	37 - 455	1e-54	Sodium/hydrogen exchanger, transmembrane

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05030 AT3G05030 AT3G05030 AT3G05030 AT5G27150
fragaria_vesca	FvH4_4g17120 FvH4_4g17120 FvH4_6g04140
malus_domestica	MD00G1051300.v1.1 MD12G1232400.v1.1 MD13G1174300.v1.1 MD16G1175800.v1.1
prunus_persica	Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.6G335800_v2.0.a1
pyrus_communis	pycom12g21480 pycom13g14990 pycom16g14730
rosa_chinensis	RchiOBHm_Chr3g0452721 RchiOBHm_Chr4g0420901 RchiOBHm_Chr5g0080441
rosa_laevigata	RLG00000007726 RLG00000025572
rosa_multiflora	Rmu_sc0002006.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363300 Rroxscaffold_6G00429730
rosa_rugosa	Rorug02G0642900 Rorug02G0642900 Rorug04G0166900 Rorug04G0167000 Rorug04G0167100
rosa_samantha	Rh3AG045100 Rh3BG047600 Rh3CG046100 Rh3DG046400 Rh4BG231400 Rh4DG226500
rosa_wichuraiana	Rw3G003530 Rw4G019760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	758, 1364
AarI	CACCTGC	1 cut(s)	526
Acc36I	ACCTGC	1 cut(s)	526
AccB1I	GGYRCC	1 cut(s)	353
AccII	CGCG	1 cut(s)	1515
AciI	CCGC	4 cut(s)	5, 165, 275, 1067
AclI	AACGTT	1 cut(s)	1656
AclWI	GGATC	2 cut(s)	798, 896
AcoI	YGGCCR	1 cut(s)	1499
AcuI	CTGAAG	1 cut(s)	316
AdeI	CACNNNGTG	1 cut(s)	919
AfaI	GTAC	3 cut(s)	187, 345, 364
AfiI	CCNNNNNNNGG	3 cut(s)	91, 1454, 1598
AgeI	ACCGGT	1 cut(s)	1591
AgsI	TTSAA	4 cut(s)	625, 933, 1025, 1077
AhdI	GACNNNNNGTC	1 cut(s)	64
AjnI	CCWGG	1 cut(s)	1051
AjuI	GAANNNNNNNTTGG	2 cut(s)	938, 970
AleI	CACNNNNGTG	1 cut(s)	1334
AluBI	AGCT	4 cut(s)	107, 639, 1112, 1241
AluI	AGCT	4 cut(s)	107, 639, 1112, 1241
Alw21I	GWGCWC	1 cut(s)	543
Alw26I	GTCTC	2 cut(s)	548, 1214
AlwI	GGATC	2 cut(s)	798, 896
Ama87I	CYCGRG	1 cut(s)	131
AoxI	GGCC	3 cut(s)	1264, 1499, 1603
ApeKI	GCWGC	5 cut(s)	509, 739, 1109, 1183, 1375
AsiGI	ACCGGT	1 cut(s)	1591
Asp700I	GAANNNNTTC	3 cut(s)	86, 223, 317
AspLEI	GCGC	1 cut(s)	434
AspS9I	GGNCC	1 cut(s)	58
AsuC2I	CCSGG	3 cut(s)	295, 1607, 1629
AsuHPI	GGTGA	6 cut(s)	154, 160, 316, 640, 1081, 1626
AvaI	CYCGRG	1 cut(s)	131
AvaII	GGWCC	1 cut(s)	58
BaeI	ACNNNNGTAYC	4 cut(s)	354, 354, 387, 387
BanI	GGYRCC	1 cut(s)	353
BarI	GAAGNNNNNNTAC	2 cut(s)	186, 218
BbsI	GAAGAC	1 cut(s)	1082
Bbv12I	GWGCWC	1 cut(s)	543
BbvI	GCAGC	5 cut(s)	496, 726, 1121, 1195, 1362
BccI	CCATC	2 cut(s)	138, 1560
BceAI	ACGGC	1 cut(s)	1514
BciT130I	CCWGG	1 cut(s)	1053
BciVI	GTATCC	1 cut(s)	382
BclI	TGATCA	2 cut(s)	207, 1296
BcnI	CCSGG	3 cut(s)	295, 1607, 1629
BcoDI	GTCTC	2 cut(s)	548, 1214
BfaI	CTAG	6 cut(s)	722, 1085, 1100, 1238, 1302, 1454
BfmI	CTRYAG	2 cut(s)	568, 1467
BfuAI	ACCTGC	1 cut(s)	526
BfuI	GTATCC	1 cut(s)	382
BglII	AGATCT	1 cut(s)	250
BisI	GCNGC	5 cut(s)	510, 740, 1110, 1184, 1376
BlsI	GCNGC	5 cut(s)	511, 741, 1111, 1185, 1377
Bme1390I	CCNGG	4 cut(s)	295, 1053, 1607, 1629
Bme18I	GGWCC	1 cut(s)	58
BmeRI	GACNNNNNGTC	1 cut(s)	64
BmeT110I	CYCGRG	1 cut(s)	131
BmgT120I	GGNCC	1 cut(s)	58
BmiI	GGNNCC	2 cut(s)	9, 355
BmrFI	CCNGG	4 cut(s)	295, 1053, 1607, 1629
BmsI	GCATC	5 cut(s)	238, 419, 592, 801, 1000
BpiI	GAAGAC	1 cut(s)	1082
BpmI	CTGGAG	1 cut(s)	210
BpuMI	CCSGG	3 cut(s)	295, 1607, 1629
Bsa29I	ATCGAT	1 cut(s)	142
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	2 cut(s)	1014, 1606
BsaWI	WCCGGW	1 cut(s)	1591
Bsc4I	CCNNNNNNNGG	3 cut(s)	91, 1454, 1598
Bse118I	RCCGGY	1 cut(s)	1591
Bse1I	ACTGG	2 cut(s)	193, 843
Bse3DI	GCAATG	1 cut(s)	666
BseBI	CCWGG	1 cut(s)	1053
BseCI	ATCGAT	1 cut(s)	142
BseDI	CCNNGG	2 cut(s)	1014, 1606
BseGI	GGATG	1 cut(s)	1015
BseLI	CCNNNNNNNGG	3 cut(s)	91, 1454, 1598
BseMI	GCAATG	1 cut(s)	666
BseMII	CTCAG	1 cut(s)	915
BseNI	ACTGG	2 cut(s)	193, 843
BseRI	GAGGAG	1 cut(s)	149
BseXI	GCAGC	5 cut(s)	496, 726, 1121, 1195, 1362
BsgI	GTGCAG	1 cut(s)	361
Bsh1236I	CGCG	1 cut(s)	1515
Bsh1285I	CGRYCG	1 cut(s)	1592
BshFI	GGCC	3 cut(s)	1266, 1501, 1605
BshNI	GGYRCC	1 cut(s)	353
BshTI	ACCGGT	1 cut(s)	1591
BshVI	ATCGAT	1 cut(s)	142
BsiEI	CGRYCG	1 cut(s)	1592
BsiHKAI	GWGCWC	1 cut(s)	543
BsiHKCI	CYCGRG	1 cut(s)	131
BsiSI	CCGG	6 cut(s)	294, 663, 1180, 1592, 1606, 1629
BslFI	GGGAC	1 cut(s)	494
BslI	CCNNNNNNNGG	3 cut(s)	91, 1454, 1598
BsmAI	GTCTC	2 cut(s)	548, 1214
BsmFI	GGGAC	1 cut(s)	494
BsmI	GAATGC	2 cut(s)	864, 1509
BsnI	GGCC	3 cut(s)	1266, 1501, 1605
Bso31I	GGTCTC	1 cut(s)	1214
BsoBI	CYCGRG	1 cut(s)	131
Bsp1286I	GDGCHC	1 cut(s)	543
Bsp68I	TCGCGA	1 cut(s)	1515
BspACI	CCGC	4 cut(s)	5, 165, 275, 1067
BspANI	GGCC	3 cut(s)	1266, 1501, 1605
BspCNI	CTCAG	1 cut(s)	916
BspDI	ATCGAT	1 cut(s)	142
BspFNI	CGCG	1 cut(s)	1515
BspHI	TCATGA	2 cut(s)	1212, 1293
BspLI	GGNNCC	2 cut(s)	9, 355
BspMI	ACCTGC	1 cut(s)	526
BspPI	GGATC	2 cut(s)	798, 896
BspQI	GCTCTTC	1 cut(s)	1099
BspT107I	GGYRCC	1 cut(s)	353
BspTNI	GGTCTC	1 cut(s)	1214
BsrDI	GCAATG	1 cut(s)	666
BsrFI	RCCGGY	1 cut(s)	1591
BsrI	ACTGG	2 cut(s)	193, 843
BssAI	RCCGGY	1 cut(s)	1591
BssECI	CCNNGG	2 cut(s)	1014, 1606
BssT1I	CCWWGG	1 cut(s)	1014
Bst2UI	CCWGG	1 cut(s)	1053
Bst4CI	ACNGT	7 cut(s)	348, 572, 874, 1049, 1315, 1333, 1549
Bst6I	CTCTTC	1 cut(s)	1099
BstAPI	GCANNNNNTGC	1 cut(s)	506
BstC8I	GCNNGC	7 cut(s)	398, 712, 951, 1281, 1406, 1503, 1507
BstDEI	CTNAG	3 cut(s)	743, 924, 1144
BstF5I	GGATG	1 cut(s)	1015
BstFNI	CGCG	1 cut(s)	1515
BstHHI	GCGC	1 cut(s)	434
BstMAI	GTCTC	2 cut(s)	548, 1214
BstMCI	CGRYCG	1 cut(s)	1592
BstMWI	GCNNNNNNNGC	2 cut(s)	497, 506
BstNI	CCWGG	1 cut(s)	1053
BstNSI	RCATGY	5 cut(s)	25, 400, 418, 953, 1408
BstSCI	CCNGG	4 cut(s)	293, 1051, 1605, 1627
BstSFI	CTRYAG	2 cut(s)	568, 1467
BstUI	CGCG	1 cut(s)	1515
BstV1I	GCAGC	5 cut(s)	496, 726, 1121, 1195, 1362
BstV2I	GAAGAC	1 cut(s)	1082
BstX2I	RGATCY	1 cut(s)	250
BstYI	RGATCY	1 cut(s)	250
Bsu15I	ATCGAT	1 cut(s)	142
BsuI	GTATCC	1 cut(s)	382
BsuRI	GGCC	3 cut(s)	1266, 1501, 1605
BsuTUI	ATCGAT	1 cut(s)	142
BtgZI	GCGATG	1 cut(s)	1538
BtsCI	GGATG	1 cut(s)	1015
BtsIMutI	CAGTG	6 cut(s)	250, 870, 1068, 1338, 1545, 1667
BtuMI	TCGCGA	1 cut(s)	1515
BveI	ACCTGC	1 cut(s)	526
Cac8I	GCNNGC	7 cut(s)	398, 712, 951, 1281, 1406, 1503, 1507
CciI	TCATGA	2 cut(s)	1212, 1293
CfoI	GCGC	1 cut(s)	434
Cfr10I	RCCGGY	1 cut(s)	1591
Cfr13I	GGNCC	1 cut(s)	58
ClaI	ATCGAT	1 cut(s)	142
Csp6I	GTAC	3 cut(s)	186, 344, 363
CspAI	ACCGGT	1 cut(s)	1591
CviQI	GTAC	3 cut(s)	186, 344, 363
DdeI	CTNAG	3 cut(s)	743, 924, 1144
DraIII	CACNNNGTG	1 cut(s)	919
DriI	GACNNNNNGTC	1 cut(s)	64
EaeI	YGGCCR	1 cut(s)	1499
Eam1104I	CTCTTC	1 cut(s)	1099
Eam1105I	GACNNNNNGTC	1 cut(s)	64
EarI	CTCTTC	1 cut(s)	1099
EciI	GGCGGA	1 cut(s)	264
Eco130I	CCWWGG	1 cut(s)	1014
Eco31I	GGTCTC	1 cut(s)	1214
Eco47I	GGWCC	1 cut(s)	58
Eco57I	CTGAAG	1 cut(s)	316
Eco88I	CYCGRG	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	1051
EcoT14I	CCWWGG	1 cut(s)	1014
ErhI	CCWWGG	1 cut(s)	1014
FalI	AAGNNNNNCTT	4 cut(s)	305, 337, 1096, 1128
FaqI	GGGAC	1 cut(s)	494
FbaI	TGATCA	2 cut(s)	207, 1296
Fnu4HI	GCNGC	5 cut(s)	510, 740, 1110, 1184, 1376
FokI	GGATG	1 cut(s)	1022
Fsp4HI	GCNGC	5 cut(s)	510, 740, 1110, 1184, 1376
FspBI	CTAG	6 cut(s)	722, 1085, 1100, 1238, 1302, 1454
GlaI	GCGC	1 cut(s)	433
GluI	GCNGC	5 cut(s)	510, 740, 1110, 1184, 1376
GsuI	CTGGAG	1 cut(s)	210
HaeIII	GGCC	3 cut(s)	1266, 1501, 1605
HapII	CCGG	6 cut(s)	294, 663, 1180, 1592, 1606, 1629
HhaI	GCGC	1 cut(s)	434
Hin6I	GCGC	1 cut(s)	432
HinP1I	GCGC	1 cut(s)	432
HincII	GTYRAC	2 cut(s)	328, 598
HindII	GTYRAC	2 cut(s)	328, 598
HinfI	GANTC	6 cut(s)	139, 155, 518, 659, 1463, 1528
HpaI	GTTAAC	2 cut(s)	328, 598
HpaII	CCGG	6 cut(s)	294, 663, 1180, 1592, 1606, 1629
HphI	GGTGA	6 cut(s)	154, 160, 316, 640, 1081, 1626
Hpy166II	GTNNAC	3 cut(s)	328, 598, 1254
Hpy188I	TCNGA	8 cut(s)	58, 122, 207, 411, 636, 1343, 1417, 1524
Hpy188III	TCNNGA	9 cut(s)	131, 470, 548, 794, 892, 933, 1213, 1294, 1514
Hpy8I	GTNNAC	3 cut(s)	328, 598, 1254
Hpy99I	CGWCG	1 cut(s)	1500
HpyAV	CCTTC	2 cut(s)	281, 1591
HpyCH4III	ACNGT	7 cut(s)	348, 572, 874, 1049, 1315, 1333, 1549
HpyCH4IV	ACGT	2 cut(s)	530, 1656
HpyCH4V	TGCA	9 cut(s)	378, 500, 512, 528, 535, 676, 734, 776, 1289
HpyF10VI	GCNNNNNNNGC	2 cut(s)	497, 506
HpyF3I	CTNAG	3 cut(s)	743, 924, 1144
HpySE526I	ACGT	2 cut(s)	530, 1656
HspAI	GCGC	1 cut(s)	432
Ksp22I	TGATCA	2 cut(s)	207, 1296
KspAI	GTTAAC	2 cut(s)	328, 598
LguI	GCTCTTC	1 cut(s)	1099
Lsp1109I	GCAGC	5 cut(s)	496, 726, 1121, 1195, 1362
LweI	GCATC	5 cut(s)	238, 419, 592, 801, 1000
MaeI	CTAG	6 cut(s)	722, 1085, 1100, 1238, 1302, 1454
MaeII	ACGT	2 cut(s)	530, 1656
MaeIII	GTNAC	4 cut(s)	36, 403, 919, 937
MboII	GAAGA	7 cut(s)	119, 194, 260, 982, 1082, 1116, 1511
MflI	RGATCY	1 cut(s)	250
MhlI	GDGCHC	1 cut(s)	543
MluCI	AATT	4 cut(s)	440, 452, 473, 845
MlyI	GAGTC	2 cut(s)	164, 1522
MroXI	GAANNNNTTC	3 cut(s)	86, 223, 317
MseI	TTAA	5 cut(s)	288, 327, 447, 597, 1536
MslI	CAYNNNNRTG	1 cut(s)	1334
MspI	CCGG	6 cut(s)	294, 663, 1180, 1592, 1606, 1629
MspR9I	CCNGG	4 cut(s)	295, 1053, 1607, 1629
Mva1269I	GAATGC	2 cut(s)	864, 1509
MvaI	CCWGG	1 cut(s)	1053
MvnI	CGCG	1 cut(s)	1515
MwoI	GCNNNNNNNGC	2 cut(s)	497, 506
NciI	CCSGG	3 cut(s)	295, 1607, 1629
NlaIV	GGNNCC	2 cut(s)	9, 355
NmuCI	GTSAC	1 cut(s)	919
NruI	TCGCGA	1 cut(s)	1515
NspI	RCATGY	5 cut(s)	25, 400, 418, 953, 1408
OliI	CACNNNNGTG	1 cut(s)	1334
PaeI	GCATGC	3 cut(s)	400, 953, 1408
PaeR7I	CTCGAG	1 cut(s)	131
PagI	TCATGA	2 cut(s)	1212, 1293
PaqCI	CACCTGC	1 cut(s)	526
PciSI	GCTCTTC	1 cut(s)	1099
PcsI	WCGNNNNNNNCGW	2 cut(s)	62, 1561
PctI	GAATGC	2 cut(s)	864, 1509
PdmI	GAANNNNTTC	3 cut(s)	86, 223, 317
PfeI	GAWTC	4 cut(s)	139, 518, 659, 1463
PfoI	TCCNGGA	1 cut(s)	1051
PinAI	ACCGGT	1 cut(s)	1591
PkrI	GCNGC	5 cut(s)	511, 741, 1111, 1185, 1377
PleI	GAGTC	2 cut(s)	163, 1522
PpsI	GAGTC	2 cut(s)	163, 1522
PsiI	TTATAA	2 cut(s)	758, 1364
Psp1406I	AACGTT	1 cut(s)	1656
Psp6I	CCWGG	1 cut(s)	1051
PspGI	CCWGG	1 cut(s)	1051
PspN4I	GGNNCC	2 cut(s)	9, 355
PspPI	GGNCC	1 cut(s)	58
PsuI	RGATCY	1 cut(s)	250
RruI	TCGCGA	1 cut(s)	1515
RsaI	GTAC	3 cut(s)	187, 345, 364
RsaNI	GTAC	3 cut(s)	186, 344, 363
RseI	CAYNNNNRTG	1 cut(s)	1334
SapI	GCTCTTC	1 cut(s)	1099
SaqAI	TTAA	5 cut(s)	288, 327, 447, 597, 1536
SatI	GCNGC	5 cut(s)	510, 740, 1110, 1184, 1376
Sau96I	GGNCC	1 cut(s)	58
SchI	GAGTC	2 cut(s)	164, 1522
ScrFI	CCNGG	4 cut(s)	295, 1053, 1607, 1629
SduI	GDGCHC	1 cut(s)	543
SfaNI	GCATC	5 cut(s)	238, 419, 592, 801, 1000
SfcI	CTRYAG	2 cut(s)	568, 1467
Sfr274I	CTCGAG	1 cut(s)	131
SinI	GGWCC	1 cut(s)	58
SlaI	CTCGAG	1 cut(s)	131
SmiMI	CAYNNNNRTG	1 cut(s)	1334
SmlI	CTYRAG	1 cut(s)	131
SmoI	CTYRAG	1 cut(s)	131
SphI	GCATGC	3 cut(s)	400, 953, 1408
Sse9I	AATT	4 cut(s)	440, 452, 473, 845
SsiI	CCGC	4 cut(s)	5, 165, 275, 1067
SspI	AATATT	2 cut(s)	504, 984
SspMI	CTAG	6 cut(s)	722, 1085, 1100, 1238, 1302, 1454
StyD4I	CCNGG	4 cut(s)	293, 1051, 1605, 1627
StyI	CCWWGG	1 cut(s)	1014
TaaI	ACNGT	7 cut(s)	348, 572, 874, 1049, 1315, 1333, 1549
TaiI	ACGT	2 cut(s)	533, 1659
TaqI	TCGA	7 cut(s)	77, 132, 142, 158, 214, 1229, 1495
TasI	AATT	4 cut(s)	440, 452, 473, 845
TatI	WGTACW	2 cut(s)	185, 343
TfiI	GAWTC	4 cut(s)	139, 518, 659, 1463
Tru1I	TTAA	5 cut(s)	288, 327, 447, 597, 1536
Tru9I	TTAA	5 cut(s)	288, 327, 447, 597, 1536
TscAI	CASTG	6 cut(s)	250, 877, 1068, 1338, 1552, 1667
TseFI	GTSAC	1 cut(s)	919
TseI	GCWGC	5 cut(s)	509, 739, 1109, 1183, 1375
Tsp45I	GTSAC	1 cut(s)	919
TspDTI	ATGAA	3 cut(s)	95, 1374, 1573
TspGWI	ACGGA	3 cut(s)	530, 803, 1660
TspRI	CASTG	6 cut(s)	250, 877, 1068, 1338, 1552, 1667
VpaK11BI	GGWCC	1 cut(s)	58
XceI	RCATGY	5 cut(s)	25, 400, 418, 953, 1408
XhoI	CTCGAG	1 cut(s)	131
XmnI	GAANNNNTTC	3 cut(s)	86, 223, 317
XspI	CTAG	6 cut(s)	722, 1085, 1100, 1238, 1302, 1454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.