Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001413

Physical Location & Seq

Genomic Coordinates Forward (+)

14361267 .. 14366044

Gene Structure 4778 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1174300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGCGGTCGATGCGAGCTCAATTGCAACGGAATCAATGGGAATTCTGATGTCCTCTGACCACAAATCGGTGGTTTCTATGAACCTTTTCGTGGCGCTTCTCTGCGCTTGTATTCTACTCGGTCATTTGCTGGAGGAAAGTCGATGGATGAATGAGTCCATCACAGCCCTTGCCATTGGACTGTGCACTGGAGTTGTGATTTTGCTCACAACTGGAGGAAAAAGCTCACATTTATTAGTGTTTAGTGAAGATCTCTTCTTTATATATCTGCTTCCGCCTATCATTTTCAATGCCGGGTTCCAGGTAAAGAAGAAGCAATTTTTTCGTAACTTCATGACTATCATGACTTTTGGTGCTGCTGGCACTCTCATATCGTTTTCCATCATATCCTTAGGTGCAATGCACTTTTTTCACAAGTTCAATATTGGTTCCCTCAAGATTGGAGATTATCTCGCACTTGGCGCAATATTTTCTGCAACCGATTCTGTTTGCACCTTACAGGTGCTTAATCAGGATGAGACGCCTTTGTTGTACAGCCTGGTTTTTGGGGAGGGTGTCGTTAATGATGCTACATCAATAGTTCTTTTCAATGCAATCCAGAGCTTCGACCTCTCTAACATCAATACAAGCACTGCTTTGCAATTTTTTGGAAGCTTTTTGTATTTGTTTGCTGCAAGTACAATGCTAGGAGTCGCAGCTGGACTATTGAGTGCATATATCATCAAGAAGCTCTACTTCGGCAGGCACTCAACTGATCGCGAAGTCGCTCTTATGATACTCATGGCTTACTTTTCATACATACTATCTGAACTATTTTATTTGAGTGCCATTCTCACTGTGTTCTTTTGTGGAATTGTTATGTCTCACTACACATGGCATAACGTGACTGAAAGTTCAAGAGTGACAACCAAGCATACTTTTGCCACTCTGTCATTTGTGGCTGAGATCTTTATCTTTCTTTATGTCGGCATGGATGCCCTGGACATTGACAAGTGGAGATTTGTGAGTGATAGCCCCGGAAAATCCATAGCAGTGAGTTCGATTCTGTTGGCGCTGGTTCTGGTTGGAAGAGCCGCCTTTGTTTTCCCCTTATCTTTCTTATCCAACTTGACTAAAAAATCTCCACAAGACAAGCTCAGTTTAAAGGAACAAGTTACAGTTTGGTGGGCGGGGCTTATGCGTGGCTCTGTTTCTATGGCACTTGCTTATAATCAGTTTAGAAGCTCTGGCCACACGGACCTGCGTGCGAATGCGACCATGATCACCAGTACCATCACAGTTGTTCTTTTCAGTACAGTGGTGTTTGGTTTGATGACTAAACCACTTGTGAGAATCTTGCTTCCTGTATCAAAACACGTCTCGAGTATGATATCTTCTGAACCATCTAGCCCTAAATCAATCACTGCGTCACTACTCAGCAATGGCCAAGATTCAGAAGTGAACCGCGGGGTGGAAGATTCAGAGGCAAACATAGGGCCCGTGGACATCCCCCGTCCAACTAGTTTACGAATGCTCCTAGGCGCTTCCTCTCACACTGTCCACCATTACTGGCGAAGATTTGACAATGCCTTCATGAGGCCTGTTTTTGGCGGGCGGGGTTTTGTGCCTGTCGTTCCAGGCTCACCTGTTGAAGAAATTGGTCATCATTGGCGTCGAGAAGGAACTAACACACGTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006873 GO:0006885 GO:0006950 GO:0006970 GO:0008150 GO:0008324 GO:0009628 GO:0009651 GO:0009987 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015078 GO:0015079 GO:0015081 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015385 GO:0015386 GO:0015491 GO:0015672 GO:0016020 GO:0019725 GO:0022804 GO:0022821 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030641 GO:0031090 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0090333 GO:0098588 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098719 GO:0098739 GO:0098771 GO:0098805 GO:0099516 GO:0099587 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

560

Amino Acids

61.38

Weight (kDa)

8.19

Isoelectric Point (pI)

45.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	32 - 444	1.1e-58	Sodium/hydrogen exchanger, transmembrane

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05030 AT3G05030 AT3G05030 AT3G05030 AT5G27150
fragaria_vesca	FvH4_4g17120 FvH4_4g17120 FvH4_6g04140
malus_domestica	MD00G1051300.v1.1 MD12G1232400.v1.1 MD13G1174300.v1.1 MD16G1175800.v1.1
prunus_persica	Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.6G335800_v2.0.a1
pyrus_communis	pycom12g21480 pycom13g14990 pycom16g14730
rosa_chinensis	RchiOBHm_Chr3g0452721 RchiOBHm_Chr4g0420901 RchiOBHm_Chr5g0080441
rosa_laevigata	RLG00000007726 RLG00000025572
rosa_multiflora	Rmu_sc0002006.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363300 Rroxscaffold_6G00429730
rosa_rugosa	Rorug02G0642900 Rorug02G0642900 Rorug04G0166900 Rorug04G0167000 Rorug04G0167100
rosa_samantha	Rh3AG045100 Rh3BG047600 Rh3CG046100 Rh3DG046400 Rh4BG231400 Rh4DG226500
rosa_wichuraiana	Rw3G003530 Rw4G019760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1209
Acc36I	ACCTGC	1 cut(s)	1248
AccII	CGCG	2 cut(s)	759, 1446
AciI	CCGC	8 cut(s)	5, 275, 1074, 1169, 1444, 1446, 1590, 1594
AcoI	YGGCCR	2 cut(s)	1228, 1423
AcsI	RAATTY	1 cut(s)	42
AcvI	CACGTG	1 cut(s)	1673
AcyI	GRCGYC	2 cut(s)	521, 1651
AfaI	GTAC	4 cut(s)	533, 679, 1270, 1294
AfiI	CCNNNNNNNGG	5 cut(s)	67, 91, 1450, 1575, 1586
AflIII	ACRYGT	3 cut(s)	1354, 1670, 1672
AgsI	TTSAA	5 cut(s)	289, 421, 589, 897, 1631
AhlI	ACTAGT	1 cut(s)	1499
AjiI	CACGTC	1 cut(s)	1357
AjnI	CCWGG	4 cut(s)	300, 537, 978, 1615
AluBI	AGCT	8 cut(s)	18, 225, 603, 654, 698, 730, 1135, 1224
AluI	AGCT	8 cut(s)	18, 225, 603, 654, 698, 730, 1135, 1224
Alw21I	GWGCWC	2 cut(s)	20, 188
Alw26I	GTCTC	3 cut(s)	512, 867, 1363
Alw44I	GTGCAC	1 cut(s)	184
Ama87I	CYCGRG	1 cut(s)	1360
AoxI	GGCC	4 cut(s)	1228, 1423, 1475, 1577
ApaI	GGGCCC	1 cut(s)	1479
ApaLI	GTGCAC	1 cut(s)	184
ApeKI	GCWGC	3 cut(s)	356, 671, 695
ApoI	RAATTY	1 cut(s)	42
Asp700I	GAANNNNTTC	1 cut(s)	86
AspA2I	CCTAGG	1 cut(s)	1516
AspLEI	GCGC	5 cut(s)	97, 107, 464, 1054, 1523
AspS9I	GGNCC	3 cut(s)	1237, 1475, 1476
AsuC2I	CCSGG	2 cut(s)	295, 1017
AsuHPI	GGTGA	2 cut(s)	1255, 1614
AvaI	CYCGRG	1 cut(s)	1360
AvaII	GGWCC	1 cut(s)	1237
AvrII	CCTAGG	1 cut(s)	1516
AxyI	CCTNAGG	1 cut(s)	391
BaeGI	GKGCMC	2 cut(s)	188, 1479
BalI	TGGCCA	2 cut(s)	1230, 1425
BanII	GRGCYC	2 cut(s)	20, 1479
BarI	GAAGNNNNNNTAC	2 cut(s)	1357, 1389
BbrPI	CACGTG	1 cut(s)	1673
Bbv12I	GWGCWC	2 cut(s)	20, 188
BbvI	GCAGC	3 cut(s)	343, 658, 707
BccI	CCATC	5 cut(s)	138, 167, 389, 1280, 1390
BcgI	CGANNNNNNTGC	4 cut(s)	305, 339, 1020, 1054
BciT130I	CCWGG	4 cut(s)	302, 539, 980, 1617
BclI	TGATCA	1 cut(s)	1260
BcnI	CCSGG	2 cut(s)	295, 1017
BcoDI	GTCTC	3 cut(s)	512, 867, 1363
BcuI	ACTAGT	1 cut(s)	1499
BfaI	CTAG	4 cut(s)	686, 1386, 1500, 1517
BfoI	RGCGCY	3 cut(s)	98, 1055, 1524
BfuAI	ACCTGC	1 cut(s)	1248
BglII	AGATCT	2 cut(s)	250, 945
BisI	GCNGC	4 cut(s)	357, 672, 696, 1074
BlnI	CCTAGG	1 cut(s)	1516
BlsI	GCNGC	4 cut(s)	358, 673, 697, 1075
Bme1390I	CCNGG	6 cut(s)	295, 302, 539, 980, 1017, 1617
Bme18I	GGWCC	1 cut(s)	1237
BmeT110I	CYCGRG	1 cut(s)	1360
BmgBI	CACGTC	1 cut(s)	1357
BmgT120I	GGNCC	3 cut(s)	1237, 1475, 1476
BmiI	GGNNCC	3 cut(s)	299, 430, 1477
BmrFI	CCNGG	6 cut(s)	295, 302, 539, 980, 1017, 1617
BmsI	GCATC	2 cut(s)	556, 964
BplI	GAGNNNNNCTC	2 cut(s)	435, 467
BpmI	CTGGAG	3 cut(s)	152, 210, 234
BpuEI	CTTGAG	1 cut(s)	419
BpuMI	CCSGG	2 cut(s)	295, 1017
BsaAI	YACGTR	1 cut(s)	1673
BsaBI	GATNNNNATC	1 cut(s)	950
BsaHI	GRCGYC	2 cut(s)	521, 1651
BsaJI	CCNNGG	5 cut(s)	978, 1015, 1444, 1479, 1516
BsaXI	ACNNNNNCTCC	2 cut(s)	1497, 1527
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 91, 1450, 1575, 1586
Bse1I	ACTGG	4 cut(s)	193, 217, 1266, 1553
Bse21I	CCTNAGG	1 cut(s)	391
Bse3DI	GCAATG	2 cut(s)	405, 1426
Bse8I	GATNNNNATC	1 cut(s)	950
BseBI	CCWGG	4 cut(s)	302, 539, 980, 1617
BseDI	CCNNGG	5 cut(s)	978, 1015, 1444, 1479, 1516
BseGI	GGATG	4 cut(s)	153, 520, 979, 1485
BseJI	GATNNNNATC	1 cut(s)	950
BseLI	CCNNNNNNNGG	5 cut(s)	67, 91, 1450, 1575, 1586
BseMI	GCAATG	2 cut(s)	405, 1426
BseMII	CTCAG	3 cut(s)	933, 1150, 1429
BseNI	ACTGG	4 cut(s)	193, 217, 1266, 1553
BseSI	GKGCMC	2 cut(s)	188, 1479
BseXI	GCAGC	3 cut(s)	343, 658, 707
Bsh1236I	CGCG	2 cut(s)	759, 1446
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	4 cut(s)	1230, 1425, 1477, 1579
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	2 cut(s)	20, 188
BsiHKCI	CYCGRG	1 cut(s)	1360
BsiSI	CCGG	2 cut(s)	294, 1017
BslI	CCNNNNNNNGG	5 cut(s)	67, 91, 1450, 1575, 1586
BsmAI	GTCTC	3 cut(s)	512, 867, 1363
BsmBI	CGTCTC	2 cut(s)	512, 1363
BsmI	GAATGC	2 cut(s)	1255, 1515
BsnI	GGCC	4 cut(s)	1230, 1425, 1477, 1579
BsoBI	CYCGRG	1 cut(s)	1360
Bsp120I	GGGCCC	1 cut(s)	1475
Bsp1286I	GDGCHC	3 cut(s)	20, 188, 1479
Bsp1407I	TGTACA	1 cut(s)	531
Bsp143I	GATC	4 cut(s)	250, 754, 945, 1260
Bsp68I	TCGCGA	1 cut(s)	759
BspACI	CCGC	8 cut(s)	5, 275, 1074, 1169, 1444, 1446, 1590, 1594
BspANI	GGCC	4 cut(s)	1230, 1425, 1477, 1579
BspCNI	CTCAG	3 cut(s)	934, 1149, 1428
BspFNI	CGCG	2 cut(s)	759, 1446
BspHI	TCATGA	3 cut(s)	333, 342, 1572
BspLI	GGNNCC	3 cut(s)	299, 430, 1477
BspMI	ACCTGC	1 cut(s)	1248
BspQI	GCTCTTC	1 cut(s)	1063
BsrDI	GCAATG	2 cut(s)	405, 1426
BsrGI	TGTACA	1 cut(s)	531
BsrI	ACTGG	4 cut(s)	193, 217, 1266, 1553
BssECI	CCNNGG	5 cut(s)	978, 1015, 1444, 1479, 1516
BssMI	GATC	4 cut(s)	250, 754, 945, 1260
BssNI	GRCGYC	2 cut(s)	521, 1651
BssT1I	CCWWGG	1 cut(s)	1516
Bst2UI	CCWGG	4 cut(s)	302, 539, 980, 1617
Bst4CI	ACNGT	6 cut(s)	183, 838, 1159, 1279, 1297, 1537
Bst6I	CTCTTC	2 cut(s)	260, 1063
BstACI	GRCGYC	2 cut(s)	521, 1651
BstAUI	TGTACA	1 cut(s)	531
BstBAI	YACGTR	1 cut(s)	1673
BstC8I	GCNNGC	5 cut(s)	16, 361, 743, 1245, 1592
BstDEI	CTNAG	4 cut(s)	391, 942, 1136, 1415
BstDSI	CCRYGG	2 cut(s)	1444, 1479
BstENI	CCTNNNNNAGG	1 cut(s)	1573
BstF5I	GGATG	4 cut(s)	153, 520, 979, 1485
BstFNI	CGCG	2 cut(s)	759, 1446
BstH2I	RGCGCY	3 cut(s)	98, 1055, 1524
BstHHI	GCGC	5 cut(s)	97, 107, 464, 1054, 1523
BstKTI	GATC	4 cut(s)	253, 757, 948, 1263
BstMAI	GTCTC	3 cut(s)	512, 867, 1363
BstMBI	GATC	4 cut(s)	250, 754, 945, 1260
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 461
BstNI	CCWGG	4 cut(s)	302, 539, 980, 1617
BstSCI	CCNGG	6 cut(s)	293, 300, 537, 978, 1015, 1615
BstSLI	GKGCMC	2 cut(s)	188, 1479
BstUI	CGCG	2 cut(s)	759, 1446
BstV1I	GCAGC	3 cut(s)	343, 658, 707
BstX2I	RGATCY	2 cut(s)	250, 945
BstYI	RGATCY	2 cut(s)	250, 945
Bsu36I	CCTNAGG	1 cut(s)	391
BsuRI	GGCC	4 cut(s)	1230, 1425, 1477, 1579
BtgI	CCRYGG	2 cut(s)	1444, 1479
BtrI	CACGTC	1 cut(s)	1357
BtsCI	GGATG	4 cut(s)	153, 520, 979, 1485
BtsI	GCAGTG	3 cut(s)	630, 1038, 1401
BtsIMutI	CAGTG	7 cut(s)	186, 630, 834, 1038, 1302, 1401, 1533
BtuMI	TCGCGA	1 cut(s)	759
BveI	ACCTGC	1 cut(s)	1248
Cac8I	GCNNGC	5 cut(s)	16, 361, 743, 1245, 1592
CciI	TCATGA	3 cut(s)	333, 342, 1572
CfoI	GCGC	5 cut(s)	97, 107, 464, 1054, 1523
Cfr13I	GGNCC	3 cut(s)	1237, 1475, 1476
Cfr42I	CCGCGG	1 cut(s)	1447
CseI	GACGC	3 cut(s)	529, 1395, 1640
Csp6I	GTAC	4 cut(s)	532, 678, 1269, 1293
CviAII	CATG	7 cut(s)	334, 343, 781, 873, 970, 1258, 1573
CviQI	GTAC	4 cut(s)	532, 678, 1269, 1293
DdeI	CTNAG	4 cut(s)	391, 942, 1136, 1415
DpnI	GATC	4 cut(s)	252, 756, 947, 1262
DpnII	GATC	4 cut(s)	250, 754, 945, 1260
DraI	TTTAAA	1 cut(s)	1143
EaeI	YGGCCR	2 cut(s)	1228, 1423
Eam1104I	CTCTTC	2 cut(s)	260, 1063
EarI	CTCTTC	2 cut(s)	260, 1063
EciI	GGCGGA	1 cut(s)	264
Ecl136II	GAGCTC	1 cut(s)	18
Eco130I	CCWWGG	1 cut(s)	1516
Eco147I	AGGCCT	1 cut(s)	1579
Eco24I	GRGCYC	2 cut(s)	20, 1479
Eco32I	GATATC	1 cut(s)	1371
Eco47I	GGWCC	1 cut(s)	1237
Eco53kI	GAGCTC	1 cut(s)	18
Eco72I	CACGTG	1 cut(s)	1673
Eco81I	CCTNAGG	1 cut(s)	391
Eco88I	CYCGRG	1 cut(s)	1360
EcoICRI	GAGCTC	1 cut(s)	18
EcoNI	CCTNNNNNAGG	1 cut(s)	1573
EcoO109I	RGGNCCY	1 cut(s)	1475
EcoRI	GAATTC	1 cut(s)	42
EcoRII	CCWGG	4 cut(s)	300, 537, 978, 1615
EcoRV	GATATC	1 cut(s)	1371
EcoT14I	CCWWGG	1 cut(s)	1516
EcoT38I	GRGCYC	2 cut(s)	20, 1479
ErhI	CCWWGG	1 cut(s)	1516
Esp3I	CGTCTC	2 cut(s)	512, 1363
FaeI	CATG	7 cut(s)	337, 346, 784, 876, 973, 1261, 1576
FalI	AAGNNNNNCTT	6 cut(s)	619, 651, 719, 751, 753, 785
FatI	CATG	7 cut(s)	333, 342, 780, 872, 969, 1257, 1572
FauI	CCCGC	4 cut(s)	1162, 1439, 1583, 1587
FbaI	TGATCA	1 cut(s)	1260
Fnu4HI	GCNGC	4 cut(s)	357, 672, 696, 1074
FokI	GGATG	4 cut(s)	160, 527, 986, 1472
FriOI	GRGCYC	2 cut(s)	20, 1479
Fsp4HI	GCNGC	4 cut(s)	357, 672, 696, 1074
FspBI	CTAG	4 cut(s)	686, 1386, 1500, 1517
GlaI	GCGC	5 cut(s)	96, 106, 463, 1053, 1522
GluI	GCNGC	4 cut(s)	357, 672, 696, 1074
GsuI	CTGGAG	3 cut(s)	152, 210, 234
HaeII	RGCGCY	3 cut(s)	98, 1055, 1524
HaeIII	GGCC	4 cut(s)	1230, 1425, 1477, 1579
HapII	CCGG	2 cut(s)	294, 1017
HgaI	GACGC	3 cut(s)	529, 1395, 1640
HhaI	GCGC	5 cut(s)	97, 107, 464, 1054, 1523
Hin1I	GRCGYC	2 cut(s)	521, 1651
Hin1II	CATG	7 cut(s)	337, 346, 784, 876, 973, 1261, 1576
Hin6I	GCGC	5 cut(s)	95, 105, 462, 1052, 1521
HinP1I	GCGC	5 cut(s)	95, 105, 462, 1052, 1521
HindIII	AAGCTT	1 cut(s)	652
HinfI	GANTC	8 cut(s)	32, 155, 482, 690, 1042, 1332, 1430, 1457
HpaII	CCGG	2 cut(s)	294, 1017
HphI	GGTGA	2 cut(s)	1255, 1614
Hpy166II	GTNNAC	5 cut(s)	186, 1441, 1483, 1505, 1540
Hpy188I	TCNGA	6 cut(s)	48, 58, 808, 1378, 1435, 1462
Hpy8I	GTNNAC	5 cut(s)	186, 1441, 1483, 1505, 1540
Hpy99I	CGWCG	1 cut(s)	1656
HpyAV	CCTTC	2 cut(s)	1579, 1652
HpyCH4III	ACNGT	6 cut(s)	183, 838, 1159, 1279, 1297, 1537
HpyCH4IV	ACGT	3 cut(s)	882, 1356, 1672
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 461
HpyF3I	CTNAG	4 cut(s)	391, 942, 1136, 1415
HpySE526I	ACGT	3 cut(s)	882, 1356, 1672
Hsp92I	GRCGYC	2 cut(s)	521, 1651
Hsp92II	CATG	7 cut(s)	337, 346, 784, 876, 973, 1261, 1576
HspAI	GCGC	5 cut(s)	95, 105, 462, 1052, 1521
Ksp22I	TGATCA	1 cut(s)	1260
KspI	CCGCGG	1 cut(s)	1447
Kzo9I	GATC	4 cut(s)	250, 754, 945, 1260
LguI	GCTCTTC	1 cut(s)	1063
LmnI	GCTCC	1 cut(s)	1518
Lsp1109I	GCAGC	3 cut(s)	343, 658, 707
LweI	GCATC	2 cut(s)	556, 964
MaeI	CTAG	4 cut(s)	686, 1386, 1500, 1517
MaeII	ACGT	3 cut(s)	882, 1356, 1672
MaeIII	GTNAC	5 cut(s)	326, 883, 901, 1153, 1407
MalI	GATC	4 cut(s)	252, 756, 947, 1262
MboI	GATC	4 cut(s)	250, 754, 945, 1260
MboII	GAAGA	8 cut(s)	247, 260, 322, 1080, 1365, 1466, 1566, 1643
MfeI	CAATTG	1 cut(s)	21
MflI	RGATCY	2 cut(s)	250, 945
MhlI	GDGCHC	3 cut(s)	20, 188, 1479
MlsI	TGGCCA	2 cut(s)	1230, 1425
MluCI	AATT	6 cut(s)	21, 42, 317, 641, 852, 1635
MluNI	TGGCCA	2 cut(s)	1230, 1425
MlyI	GAGTC	2 cut(s)	164, 699
MmeI	TCCRAC	3 cut(s)	1045, 1128, 1520
MnlI	CCTC	9 cut(s)	64, 127, 209, 443, 544, 620, 1456, 1537, 1569
Mox20I	TGGCCA	2 cut(s)	1230, 1425
MroXI	GAANNNNTTC	1 cut(s)	86
MscI	TGGCCA	2 cut(s)	1230, 1425
MseI	TTAA	3 cut(s)	507, 561, 1142
MslI	CAYNNNNRTG	1 cut(s)	1031
Msp20I	TGGCCA	2 cut(s)	1230, 1425
MspA1I	CMGCKG	2 cut(s)	698, 1446
MspI	CCGG	2 cut(s)	294, 1017
MspR9I	CCNGG	6 cut(s)	295, 302, 539, 980, 1017, 1617
MunI	CAATTG	1 cut(s)	21
Mva1269I	GAATGC	2 cut(s)	1255, 1515
MvaI	CCWGG	4 cut(s)	302, 539, 980, 1617
MvnI	CGCG	2 cut(s)	759, 1446
MwoI	GCNNNNNNNGC	2 cut(s)	11, 461
NciI	CCSGG	2 cut(s)	295, 1017
NdeII	GATC	4 cut(s)	250, 754, 945, 1260
NlaIII	CATG	7 cut(s)	337, 346, 784, 876, 973, 1261, 1576
NlaIV	GGNNCC	3 cut(s)	299, 430, 1477
NmuCI	GTSAC	3 cut(s)	883, 901, 1407
NruI	TCGCGA	1 cut(s)	759
PaeR7I	CTCGAG	1 cut(s)	1360
PagI	TCATGA	3 cut(s)	333, 342, 1572
PceI	AGGCCT	1 cut(s)	1579
PciSI	GCTCTTC	1 cut(s)	1063
PctI	GAATGC	2 cut(s)	1255, 1515
PdmI	GAANNNNTTC	1 cut(s)	86
PfeI	GAWTC	6 cut(s)	32, 482, 1042, 1332, 1430, 1457
PkrI	GCNGC	4 cut(s)	358, 673, 697, 1075
PleI	GAGTC	2 cut(s)	163, 698
PmaCI	CACGTG	1 cut(s)	1673
PmlI	CACGTG	1 cut(s)	1673
PpsI	GAGTC	2 cut(s)	163, 698
Ppu21I	YACGTR	1 cut(s)	1673
PsiI	TTATAA	1 cut(s)	1209
Psp124BI	GAGCTC	1 cut(s)	20
Psp6I	CCWGG	4 cut(s)	300, 537, 978, 1615
PspCI	CACGTG	1 cut(s)	1673
PspGI	CCWGG	4 cut(s)	300, 537, 978, 1615
PspN4I	GGNNCC	3 cut(s)	299, 430, 1477
PspOMI	GGGCCC	1 cut(s)	1475
PspPI	GGNCC	3 cut(s)	1237, 1475, 1476
PsuI	RGATCY	2 cut(s)	250, 945
PvuII	CAGCTG	1 cut(s)	698
RruI	TCGCGA	1 cut(s)	759
RsaI	GTAC	4 cut(s)	533, 679, 1270, 1294
RsaNI	GTAC	4 cut(s)	532, 678, 1269, 1293
RseI	CAYNNNNRTG	1 cut(s)	1031
SacI	GAGCTC	1 cut(s)	20
SacII	CCGCGG	1 cut(s)	1447
SapI	GCTCTTC	1 cut(s)	1063
SaqAI	TTAA	3 cut(s)	507, 561, 1142
SatI	GCNGC	4 cut(s)	357, 672, 696, 1074
Sau3AI	GATC	4 cut(s)	250, 754, 945, 1260
Sau96I	GGNCC	3 cut(s)	1237, 1475, 1476
SchI	GAGTC	2 cut(s)	164, 699
ScrFI	CCNGG	6 cut(s)	295, 302, 539, 980, 1017, 1617
SduI	GDGCHC	3 cut(s)	20, 188, 1479
SfaNI	GCATC	2 cut(s)	556, 964
Sfr274I	CTCGAG	1 cut(s)	1360
Sfr303I	CCGCGG	1 cut(s)	1447
SgrBI	CCGCGG	1 cut(s)	1447
SinI	GGWCC	1 cut(s)	1237
SlaI	CTCGAG	1 cut(s)	1360
SmiMI	CAYNNNNRTG	1 cut(s)	1031
SmlI	CTYRAG	2 cut(s)	434, 1360
SmoI	CTYRAG	2 cut(s)	434, 1360
SpeI	ACTAGT	1 cut(s)	1499
Sse9I	AATT	6 cut(s)	21, 42, 317, 641, 852, 1635
SseBI	AGGCCT	1 cut(s)	1579
SsiI	CCGC	8 cut(s)	5, 275, 1074, 1169, 1444, 1446, 1590, 1594
SspI	AATATT	2 cut(s)	424, 468
SspMI	CTAG	4 cut(s)	686, 1386, 1500, 1517
SstI	GAGCTC	1 cut(s)	20
StuI	AGGCCT	1 cut(s)	1579
StyD4I	CCNGG	6 cut(s)	293, 300, 537, 978, 1015, 1615
StyI	CCWWGG	1 cut(s)	1516
TaaI	ACNGT	6 cut(s)	183, 838, 1159, 1279, 1297, 1537
TaiI	ACGT	3 cut(s)	885, 1359, 1675
TaqI	TCGA	6 cut(s)	9, 142, 606, 1040, 1361, 1654
TaqII	GACCGA	1 cut(s)	110
TasI	AATT	6 cut(s)	21, 42, 317, 641, 852, 1635
TatI	WGTACW	3 cut(s)	531, 677, 1292
TauI	GCSGC	1 cut(s)	1076
TfiI	GAWTC	6 cut(s)	32, 482, 1042, 1332, 1430, 1457
Tru1I	TTAA	3 cut(s)	507, 561, 1142
Tru9I	TTAA	3 cut(s)	507, 561, 1142
TscAI	CASTG	7 cut(s)	193, 637, 841, 1038, 1302, 1408, 1540
TseFI	GTSAC	3 cut(s)	883, 901, 1407
TseI	GCWGC	3 cut(s)	356, 671, 695
Tsp45I	GTSAC	3 cut(s)	883, 901, 1407
TspDTI	ATGAA	5 cut(s)	95, 164, 322, 783, 1561
TspGWI	ACGGA	2 cut(s)	44, 1250
TspRI	CASTG	7 cut(s)	193, 637, 841, 1038, 1302, 1408, 1540
VneI	GTGCAC	1 cut(s)	184
VpaK11BI	GGWCC	1 cut(s)	1237
XagI	CCTNNNNNAGG	1 cut(s)	1573
XapI	RAATTY	1 cut(s)	42
XhoI	CTCGAG	1 cut(s)	1360
XmaJI	CCTAGG	1 cut(s)	1516
XmnI	GAANNNNTTC	1 cut(s)	86
XspI	CTAG	4 cut(s)	686, 1386, 1500, 1517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.