Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0001413

Physical Location & Seq

Genomic Coordinates Reverse (-)

22629765 .. 22633069

Gene Structure 3305 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g21480.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGAACCTTTTCGTGGCGCTACTTTTAGCTTGTATTGTGATCGGACATCTTCTCGAGGAGAATCGATGGGTGAATGAGTCGATCACCGCCCTTTTGATTGGGGTATGTACTGGAGTAGTTATTCTTCTGATCAGTCGAGGAAAAAGTTCGCGTCTTTTGGTTTTCAGTGAAGATCTTTTCTTTATATACCTCCTTCCACCTATTATTTTTAATGCCGGGTTTCAGGTGAAAAAGAAGCAGTTCTTTGTTAACTTCAGCACCATTGTACTGTTTGGTGCCATTGGTACATTAGTATCCTGCACTATCATAGCATTAGGCGCTACACAATTCTTTAAGAAATTGGATATAGGAACTCTAGAATTGGGGGACTTTCTTGCAATTGGTGCAATATTTGCTGCAACGGATTCTGTTTGCACATTGCAGGTGCTCAATCAAGATGAGACACCTTTACTCTACAGTCTTGTGTTCGGGGAGGGTGTCGTTAACGATGCGACATCTGTGGTTCTTTTCAATGCTATTCAGAGCTTTGATCTCACCCACCTTGATTCCGGCATTGCCTTGCACTTTCTGGGAAACTTCTTATATTTGTTTTTCGCAAGCACCATGCTAGGAGTGTTTGCAGGGCTGCTTAGTGCTTACATTATCAAAAAGCTTTATTTTGGAAGGCACTCTACGGATCGTGAGGTTGCTCTTATGATGCTCATGGCATACCTGTCATATATACTGGCTGAATTATTCTATTTGAGTGGCATTCTCACCGTGTTCTTTTGTGGGATCGTGATGTCGCATTACACTTGGCACAATGTGACTGAGAGTTCAAGAGTTACGACCAAGCATGCTTTCGCAACCTTGTCATTTGTTGCCGAAATATTTATCTTCCTTTATGTTGGTATGGATGCCTTGGACATTGAAAAGTGGAGATTTGTAAGCGACAGTCCTGGAACATCTGTGGCGGTGAGTTCAATACTGCTAGGTCTTCTTATGCTAGGAAGAGCAGCTTTTGTTTTCCCCCTATCATTTTTGTCCAACTTAGCAAAGAAAAACCAACATGAGAAAATCAGCCTCCAGCAGCAAGTGATAATATGGTGGGCTGGTCTCATGAGAGGTGCTGTTTCGATAGCACTAGCTTACAATCAGTTTACGAGGTCAGGCCACACACAGTTGCGAGCAAATGCAATCATGATCACTAGCACGATAACTGTTGTTCTTGTCAGCACAGTGGTGTTCGGATTGATGACAAAACCTCTTATAAGGTTCTTGCTGCCTCATTCATCAAAACAAACAACCAGCATGCTGTCATCAGAACCAACCACTCCAAAATCAATCATTATCCCACTTCTGGGGCAGGATTCTGTAGATGATCTCGCTGGCCATGATATTCGACGGCCAGCCAGCATTCGCGATCTTCTGACGACTCCATTTAATAGGCACACTGTCCATCGCTATTGGCGTAAGTTTGATAACGCCTTCATGCGACCAGTGTTTGGAGGCCGGGGTTTTGTTCCCTTTGTTCCCGGCTCACCAACTGAACGGAGCAACAACGTTCAGTGGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006873 GO:0006885 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009628 GO:0009651 GO:0009987 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015078 GO:0015079 GO:0015081 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015385 GO:0015386 GO:0015491 GO:0015672 GO:0016020 GO:0019725 GO:0022804 GO:0022821 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030641 GO:0031090 GO:0032501 GO:0032502 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0090333 GO:0098588 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098719 GO:0098739 GO:0098771 GO:0098805 GO:0099402 GO:0099516 GO:0099587 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

519

Amino Acids

57.51

Weight (kDa)

8.85

Isoelectric Point (pI)

34.14

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05030 AT3G05030 AT3G05030 AT3G05030 AT5G27150
fragaria_vesca	FvH4_4g17120 FvH4_4g17120 FvH4_6g04140
malus_domestica	MD00G1051300.v1.1 MD12G1232400.v1.1 MD13G1174300.v1.1 MD16G1175800.v1.1
prunus_persica	Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.6G335800_v2.0.a1
pyrus_communis	pycom12g21480 pycom13g14990 pycom16g14730
rosa_chinensis	RchiOBHm_Chr3g0452721 RchiOBHm_Chr4g0420901 RchiOBHm_Chr5g0080441
rosa_laevigata	RLG00000007726 RLG00000025572
rosa_multiflora	Rmu_sc0002006.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363300 Rroxscaffold_6G00429730
rosa_rugosa	Rorug02G0642900 Rorug02G0642900 Rorug04G0166900 Rorug04G0167000 Rorug04G0167100
rosa_samantha	Rh3AG045100 Rh3BG047600 Rh3CG046100 Rh3DG046400 Rh4BG231400 Rh4DG226500
rosa_wichuraiana	Rw3G003530 Rw4G019760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1250
AarI	CACCTGC	1 cut(s)	412
Acc36I	ACCTGC	1 cut(s)	412
AccB1I	GGYRCC	1 cut(s)	275
AccB7I	CCANNNNNTGG	1 cut(s)	1484
AccII	CGCG	2 cut(s)	151, 1401
AciI	CCGC	2 cut(s)	87, 953
AclI	AACGTT	1 cut(s)	1542
AclWI	GGATC	2 cut(s)	684, 782
AcoI	YGGCCR	2 cut(s)	1369, 1385
AcuI	CTGAAG	1 cut(s)	238
AdeI	CACNNNGTG	1 cut(s)	805
AfaI	GTAC	3 cut(s)	109, 267, 286
AfiI	CCNNNNNNNGG	4 cut(s)	13, 1339, 1340, 1484
AgsI	TTSAA	4 cut(s)	511, 819, 911, 963
AjnI	CCWGG	1 cut(s)	937
AjuI	GAANNNNNNNTTGG	2 cut(s)	824, 856
AleI	CACNNNNGTG	1 cut(s)	1220
AluBI	AGCT	5 cut(s)	29, 525, 652, 998, 1127
AluI	AGCT	5 cut(s)	29, 525, 652, 998, 1127
Alw21I	GWGCWC	1 cut(s)	429
Alw26I	GTCTC	2 cut(s)	434, 1100
AlwI	GGATC	2 cut(s)	684, 782
Ama87I	CYCGRG	1 cut(s)	53
AoxI	GGCC	4 cut(s)	1150, 1369, 1385, 1489
ApeKI	GCWGC	5 cut(s)	395, 625, 995, 1069, 1261
Asp700I	GAANNNNTTC	3 cut(s)	8, 145, 239
AspLEI	GCGC	2 cut(s)	19, 320
AsuC2I	CCSGG	3 cut(s)	217, 1493, 1515
AsuHPI	GGTGA	7 cut(s)	76, 82, 238, 526, 748, 967, 1512
AvaI	CYCGRG	1 cut(s)	53
BaeI	ACNNNNGTAYC	4 cut(s)	276, 276, 309, 309
BalI	TGGCCA	1 cut(s)	1371
BanI	GGYRCC	1 cut(s)	275
BarI	GAAGNNNNNNTAC	4 cut(s)	108, 140, 655, 687
BbsI	GAAGAC	1 cut(s)	968
Bbv12I	GWGCWC	1 cut(s)	429
BbvI	GCAGC	5 cut(s)	382, 612, 1007, 1081, 1248
BccI	CCATC	2 cut(s)	60, 1446
BceAI	ACGGC	1 cut(s)	1400
BciT130I	CCWGG	1 cut(s)	939
BciVI	GTATCC	1 cut(s)	304
BclI	TGATCA	2 cut(s)	129, 1182
BcnI	CCSGG	3 cut(s)	217, 1493, 1515
BcoDI	GTCTC	2 cut(s)	434, 1100
BfaI	CTAG	6 cut(s)	356, 608, 971, 986, 1124, 1188
BfmI	CTRYAG	2 cut(s)	454, 1353
BfoI	RGCGCY	2 cut(s)	20, 321
BfuAI	ACCTGC	1 cut(s)	412
BfuI	GTATCC	1 cut(s)	304
BglII	AGATCT	1 cut(s)	172
BisI	GCNGC	5 cut(s)	396, 626, 996, 1070, 1262
BlsI	GCNGC	5 cut(s)	397, 627, 997, 1071, 1263
Bme1390I	CCNGG	4 cut(s)	217, 939, 1493, 1515
BmeT110I	CYCGRG	1 cut(s)	53
BmiI	GGNNCC	1 cut(s)	277
BmrFI	CCNGG	4 cut(s)	217, 939, 1493, 1515
BmsI	GCATC	3 cut(s)	478, 687, 886
BpiI	GAAGAC	1 cut(s)	968
BpmI	CTGGAG	2 cut(s)	132, 1049
BpuMI	CCSGG	3 cut(s)	217, 1493, 1515
Bsa29I	ATCGAT	1 cut(s)	64
BsaI	GGTCTC	1 cut(s)	1100
BsaJI	CCNNGG	2 cut(s)	900, 1492
Bsc4I	CCNNNNNNNGG	4 cut(s)	13, 1339, 1340, 1484
Bse1I	ACTGG	3 cut(s)	115, 729, 1478
Bse3DI	GCAATG	2 cut(s)	416, 552
BseBI	CCWGG	1 cut(s)	939
BseCI	ATCGAT	1 cut(s)	64
BseDI	CCNNGG	2 cut(s)	900, 1492
BseGI	GGATG	1 cut(s)	901
BseLI	CCNNNNNNNGG	4 cut(s)	13, 1339, 1340, 1484
BseMI	GCAATG	2 cut(s)	416, 552
BseMII	CTCAG	1 cut(s)	801
BseNI	ACTGG	3 cut(s)	115, 729, 1478
BseRI	GAGGAG	1 cut(s)	71
BseXI	GCAGC	5 cut(s)	382, 612, 1007, 1081, 1248
BsgI	GTGCAG	1 cut(s)	283
Bsh1236I	CGCG	2 cut(s)	151, 1401
BshFI	GGCC	4 cut(s)	1152, 1371, 1387, 1491
BshNI	GGYRCC	1 cut(s)	275
BshVI	ATCGAT	1 cut(s)	64
BsiHKAI	GWGCWC	1 cut(s)	429
BsiHKCI	CYCGRG	1 cut(s)	53
BsiSI	CCGG	4 cut(s)	216, 549, 1492, 1515
BslFI	GGGAC	1 cut(s)	380
BslI	CCNNNNNNNGG	4 cut(s)	13, 1339, 1340, 1484
BsmAI	GTCTC	2 cut(s)	434, 1100
BsmFI	GGGAC	1 cut(s)	380
BsmI	GAATGC	2 cut(s)	750, 1395
BsnI	GGCC	4 cut(s)	1152, 1371, 1387, 1491
Bso31I	GGTCTC	1 cut(s)	1100
BsoBI	CYCGRG	1 cut(s)	53
Bsp1286I	GDGCHC	1 cut(s)	429
Bsp68I	TCGCGA	1 cut(s)	1401
BspACI	CCGC	2 cut(s)	87, 953
BspANI	GGCC	4 cut(s)	1152, 1371, 1387, 1491
BspCNI	CTCAG	1 cut(s)	802
BspDI	ATCGAT	1 cut(s)	64
BspFNI	CGCG	2 cut(s)	151, 1401
BspHI	TCATGA	2 cut(s)	1098, 1179
BspLI	GGNNCC	1 cut(s)	277
BspMI	ACCTGC	1 cut(s)	412
BspPI	GGATC	2 cut(s)	684, 782
BspQI	GCTCTTC	1 cut(s)	985
BspT107I	GGYRCC	1 cut(s)	275
BspTNI	GGTCTC	1 cut(s)	1100
BsrDI	GCAATG	2 cut(s)	416, 552
BsrI	ACTGG	3 cut(s)	115, 729, 1478
BssECI	CCNNGG	2 cut(s)	900, 1492
BssT1I	CCWWGG	1 cut(s)	900
Bst2UI	CCWGG	1 cut(s)	939
Bst4CI	ACNGT	8 cut(s)	270, 458, 760, 935, 1161, 1201, 1219, 1435
Bst6I	CTCTTC	1 cut(s)	985
BstAPI	GCANNNNNTGC	2 cut(s)	383, 392
BstC8I	GCNNGC	7 cut(s)	598, 837, 1167, 1292, 1369, 1389, 1393
BstDEI	CTNAG	3 cut(s)	629, 810, 1030
BstF5I	GGATG	1 cut(s)	901
BstFNI	CGCG	2 cut(s)	151, 1401
BstH2I	RGCGCY	2 cut(s)	20, 321
BstHHI	GCGC	2 cut(s)	19, 320
BstMAI	GTCTC	2 cut(s)	434, 1100
BstMWI	GCNNNNNNNGC	3 cut(s)	317, 383, 392
BstNI	CCWGG	1 cut(s)	939
BstNSI	RCATGY	2 cut(s)	839, 1294
BstSCI	CCNGG	4 cut(s)	215, 937, 1491, 1513
BstSFI	CTRYAG	2 cut(s)	454, 1353
BstUI	CGCG	2 cut(s)	151, 1401
BstV1I	GCAGC	5 cut(s)	382, 612, 1007, 1081, 1248
BstV2I	GAAGAC	1 cut(s)	968
BstX2I	RGATCY	1 cut(s)	172
BstYI	RGATCY	1 cut(s)	172
Bsu15I	ATCGAT	1 cut(s)	64
BsuI	GTATCC	1 cut(s)	304
BsuRI	GGCC	4 cut(s)	1152, 1371, 1387, 1491
BsuTUI	ATCGAT	1 cut(s)	64
BtgZI	GCGATG	1 cut(s)	1424
BtsCI	GGATG	1 cut(s)	901
BtsIMutI	CAGTG	5 cut(s)	172, 1224, 1431, 1485, 1553
BtuMI	TCGCGA	1 cut(s)	1401
BveI	ACCTGC	1 cut(s)	412
Cac8I	GCNNGC	7 cut(s)	598, 837, 1167, 1292, 1369, 1389, 1393
CciI	TCATGA	2 cut(s)	1098, 1179
CfoI	GCGC	2 cut(s)	19, 320
ClaI	ATCGAT	1 cut(s)	64
CseI	GACGC	1 cut(s)	140
Csp6I	GTAC	3 cut(s)	108, 266, 285
CviAII	CATG	9 cut(s)	604, 703, 836, 1049, 1099, 1180, 1291, 1373, 1471
CviQI	GTAC	3 cut(s)	108, 266, 285
DdeI	CTNAG	3 cut(s)	629, 810, 1030
DraIII	CACNNNGTG	1 cut(s)	805
EaeI	YGGCCR	2 cut(s)	1369, 1385
Eam1104I	CTCTTC	1 cut(s)	985
EarI	CTCTTC	1 cut(s)	985
Eco130I	CCWWGG	1 cut(s)	900
Eco31I	GGTCTC	1 cut(s)	1100
Eco57I	CTGAAG	1 cut(s)	238
Eco88I	CYCGRG	1 cut(s)	53
EcoRII	CCWGG	1 cut(s)	937
EcoT14I	CCWWGG	1 cut(s)	900
ErhI	CCWWGG	1 cut(s)	900
FaeI	CATG	9 cut(s)	607, 706, 839, 1052, 1102, 1183, 1294, 1376, 1474
FalI	AAGNNNNNCTT	4 cut(s)	227, 259, 982, 1014
FaqI	GGGAC	1 cut(s)	380
FatI	CATG	9 cut(s)	603, 702, 835, 1048, 1098, 1179, 1290, 1372, 1470
FbaI	TGATCA	2 cut(s)	129, 1182
Fnu4HI	GCNGC	5 cut(s)	396, 626, 996, 1070, 1262
FokI	GGATG	1 cut(s)	908
Fsp4HI	GCNGC	5 cut(s)	396, 626, 996, 1070, 1262
FspBI	CTAG	6 cut(s)	356, 608, 971, 986, 1124, 1188
GlaI	GCGC	2 cut(s)	18, 319
GluI	GCNGC	5 cut(s)	396, 626, 996, 1070, 1262
GsuI	CTGGAG	2 cut(s)	132, 1049
HaeII	RGCGCY	2 cut(s)	20, 321
HaeIII	GGCC	4 cut(s)	1152, 1371, 1387, 1491
HapII	CCGG	4 cut(s)	216, 549, 1492, 1515
HgaI	GACGC	1 cut(s)	140
HhaI	GCGC	2 cut(s)	19, 320
Hin1II	CATG	9 cut(s)	607, 706, 839, 1052, 1102, 1183, 1294, 1376, 1474
Hin6I	GCGC	2 cut(s)	17, 318
HinP1I	GCGC	2 cut(s)	17, 318
HincII	GTYRAC	2 cut(s)	250, 484
HindII	GTYRAC	2 cut(s)	250, 484
HindIII	AAGCTT	1 cut(s)	650
HinfI	GANTC	6 cut(s)	61, 77, 404, 545, 1349, 1414
HpaI	GTTAAC	2 cut(s)	250, 484
HpaII	CCGG	4 cut(s)	216, 549, 1492, 1515
HphI	GGTGA	7 cut(s)	76, 82, 238, 526, 748, 967, 1512
Hpy166II	GTNNAC	3 cut(s)	250, 484, 1140
Hpy188I	TCNGA	6 cut(s)	44, 129, 522, 1229, 1303, 1410
Hpy188III	TCNNGA	9 cut(s)	53, 356, 434, 680, 778, 819, 1099, 1180, 1400
Hpy8I	GTNNAC	3 cut(s)	250, 484, 1140
Hpy99I	CGWCG	1 cut(s)	1386
HpyAV	CCTTC	3 cut(s)	203, 657, 1477
HpyCH4III	ACNGT	8 cut(s)	270, 458, 760, 935, 1161, 1201, 1219, 1435
HpyCH4IV	ACGT	1 cut(s)	1542
HpyCH4V	TGCA	9 cut(s)	300, 377, 386, 398, 414, 421, 562, 620, 1175
HpyF10VI	GCNNNNNNNGC	3 cut(s)	317, 383, 392
HpyF3I	CTNAG	3 cut(s)	629, 810, 1030
HpySE526I	ACGT	1 cut(s)	1542
Hsp92II	CATG	9 cut(s)	607, 706, 839, 1052, 1102, 1183, 1294, 1376, 1474
HspAI	GCGC	2 cut(s)	17, 318
Ksp22I	TGATCA	2 cut(s)	129, 1182
KspAI	GTTAAC	2 cut(s)	250, 484
LguI	GCTCTTC	1 cut(s)	985
LmnI	GCTCC	1 cut(s)	1533
Lsp1109I	GCAGC	5 cut(s)	382, 612, 1007, 1081, 1248
LweI	GCATC	3 cut(s)	478, 687, 886
MaeI	CTAG	6 cut(s)	356, 608, 971, 986, 1124, 1188
MaeII	ACGT	1 cut(s)	1542
MaeIII	GTNAC	2 cut(s)	805, 823
MboII	GAAGA	7 cut(s)	41, 116, 182, 868, 968, 1002, 1397
MfeI	CAATTG	1 cut(s)	378
MflI	RGATCY	1 cut(s)	172
MhlI	GDGCHC	1 cut(s)	429
MlsI	TGGCCA	1 cut(s)	1371
MluCI	AATT	5 cut(s)	326, 338, 359, 378, 731
MluNI	TGGCCA	1 cut(s)	1371
MlyI	GAGTC	2 cut(s)	86, 1408
MmeI	TCCRAC	1 cut(s)	1050
Mox20I	TGGCCA	1 cut(s)	1371
MroXI	GAANNNNTTC	3 cut(s)	8, 145, 239
MscI	TGGCCA	1 cut(s)	1371
MseI	TTAA	5 cut(s)	210, 249, 333, 483, 1422
MslI	CAYNNNNRTG	1 cut(s)	1220
Msp20I	TGGCCA	1 cut(s)	1371
MspI	CCGG	4 cut(s)	216, 549, 1492, 1515
MspR9I	CCNGG	4 cut(s)	217, 939, 1493, 1515
MunI	CAATTG	1 cut(s)	378
Mva1269I	GAATGC	2 cut(s)	750, 1395
MvaI	CCWGG	1 cut(s)	939
MvnI	CGCG	2 cut(s)	151, 1401
MwoI	GCNNNNNNNGC	3 cut(s)	317, 383, 392
NciI	CCSGG	3 cut(s)	217, 1493, 1515
NlaIII	CATG	9 cut(s)	607, 706, 839, 1052, 1102, 1183, 1294, 1376, 1474
NlaIV	GGNNCC	1 cut(s)	277
NmuCI	GTSAC	1 cut(s)	805
NruI	TCGCGA	1 cut(s)	1401
NspI	RCATGY	2 cut(s)	839, 1294
OliI	CACNNNNGTG	1 cut(s)	1220
PaeI	GCATGC	2 cut(s)	839, 1294
PaeR7I	CTCGAG	1 cut(s)	53
PagI	TCATGA	2 cut(s)	1098, 1179
PaqCI	CACCTGC	1 cut(s)	412
PciSI	GCTCTTC	1 cut(s)	985
PcsI	WCGNNNNNNNCGW	1 cut(s)	1447
PctI	GAATGC	2 cut(s)	750, 1395
PdmI	GAANNNNTTC	3 cut(s)	8, 145, 239
PfeI	GAWTC	4 cut(s)	61, 404, 545, 1349
PflMI	CCANNNNNTGG	1 cut(s)	1484
PfoI	TCCNGGA	1 cut(s)	937
PkrI	GCNGC	5 cut(s)	397, 627, 997, 1071, 1263
PleI	GAGTC	2 cut(s)	85, 1408
PpsI	GAGTC	2 cut(s)	85, 1408
PsiI	TTATAA	1 cut(s)	1250
Psp1406I	AACGTT	1 cut(s)	1542
Psp6I	CCWGG	1 cut(s)	937
PspGI	CCWGG	1 cut(s)	937
PspN4I	GGNNCC	1 cut(s)	277
PsuI	RGATCY	1 cut(s)	172
RruI	TCGCGA	1 cut(s)	1401
RsaI	GTAC	3 cut(s)	109, 267, 286
RsaNI	GTAC	3 cut(s)	108, 266, 285
RseI	CAYNNNNRTG	1 cut(s)	1220
SapI	GCTCTTC	1 cut(s)	985
SaqAI	TTAA	5 cut(s)	210, 249, 333, 483, 1422
SatI	GCNGC	5 cut(s)	396, 626, 996, 1070, 1262
SchI	GAGTC	2 cut(s)	86, 1408
ScrFI	CCNGG	4 cut(s)	217, 939, 1493, 1515
SduI	GDGCHC	1 cut(s)	429
SfaNI	GCATC	3 cut(s)	478, 687, 886
SfcI	CTRYAG	2 cut(s)	454, 1353
Sfr274I	CTCGAG	1 cut(s)	53
SlaI	CTCGAG	1 cut(s)	53
SmiMI	CAYNNNNRTG	1 cut(s)	1220
SmlI	CTYRAG	1 cut(s)	53
SmoI	CTYRAG	1 cut(s)	53
SphI	GCATGC	2 cut(s)	839, 1294
Sse9I	AATT	5 cut(s)	326, 338, 359, 378, 731
SsiI	CCGC	2 cut(s)	87, 953
SspI	AATATT	2 cut(s)	390, 870
SspMI	CTAG	6 cut(s)	356, 608, 971, 986, 1124, 1188
StyD4I	CCNGG	4 cut(s)	215, 937, 1491, 1513
StyI	CCWWGG	1 cut(s)	900
TaaI	ACNGT	8 cut(s)	270, 458, 760, 935, 1161, 1201, 1219, 1435
TaiI	ACGT	1 cut(s)	1545
TaqI	TCGA	6 cut(s)	54, 64, 80, 136, 1115, 1381
TasI	AATT	5 cut(s)	326, 338, 359, 378, 731
TatI	WGTACW	2 cut(s)	107, 265
TfiI	GAWTC	4 cut(s)	61, 404, 545, 1349
Tru1I	TTAA	5 cut(s)	210, 249, 333, 483, 1422
Tru9I	TTAA	5 cut(s)	210, 249, 333, 483, 1422
TscAI	CASTG	5 cut(s)	172, 1224, 1438, 1485, 1553
TseFI	GTSAC	1 cut(s)	805
TseI	GCWGC	5 cut(s)	395, 625, 995, 1069, 1261
Tsp45I	GTSAC	1 cut(s)	805
TspDTI	ATGAA	3 cut(s)	17, 1260, 1459
TspGWI	ACGGA	3 cut(s)	416, 689, 1546
TspRI	CASTG	5 cut(s)	172, 1224, 1438, 1485, 1553
Van91I	CCANNNNNTGG	1 cut(s)	1484
XbaI	TCTAGA	1 cut(s)	355
XceI	RCATGY	2 cut(s)	839, 1294
XhoI	CTCGAG	1 cut(s)	53
XmnI	GAANNNNTTC	3 cut(s)	8, 145, 239
XspI	CTAG	6 cut(s)	356, 608, 971, 986, 1124, 1188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.