Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001413

Physical Location & Seq

Genomic Coordinates Forward (+)

14911647 .. 14916135

Gene Structure 4489 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1175800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGGCGGTCGATGCGAGCACAATTGCAACGGAATCAATGAAAATTCTGATGGCCTCCGACCACACATCAGTGGTTTCGATGAACCTTTTCGTGGCGCTTCTCTGCGCTTGTATTCTACTTGGTCATTTGCTGGAGGAAAGTCGATGGATGAACGAGTCCATCACAGCCCTTGTAATTGGACTGTGCACAGGAGTTGTAATTTTGCTCACAACTGGAGGAAAAAGCTCACATTTGTTGGTGTTCAGTGAAGATCTCTTCTTTATATATCTGCTTCCGCCTATCATTTTTAATGCCGGGTTCCAGGTCAAGAAGAAGCAATTTTTTCGTAATTTCATGACTATCGTGACGTTTGGTGCTGTTGGAACTCTCATATCCTTTGCCATCGTATCCTTAGGTGCAATGCACTTTTTTCACAAGTTCAGTGTCGGTTCCCTCAAGATTGGAGATTATCTTGCACTTGGCGCGATATTTTCTGCAACCGATTCTGTTTGCACCTTACAGGTGCTGAATCAGGATGAAACGCCTTTGTTGTACAGCCTGGTTTTTGGGGAAGGTGTCGTTAATGATGCTACATCAATAGTGCTTTTCAATGCGATTCAGAGCTTCGACCTCTCTCACATCAATACAAGCACTGCTTTGCAATTTTTTGGAAACTTTTTATATTTGTTTGTTACAAGTACAATCCTGGGAGTCGCAGCTGGACTACTAAGTGCATTTATCATCAAGAAGCTCTACTTCGGCAGGCACTCAACTGATCGCGAAGTCGCTCTTATGATACTCATGGCTTACCTGTCATACGTGCTGTCTGAACTATGTTATTTGAGTGCCATTCTCACTGTGTTCTTTTGCGGAATTGTTATGTCTCACTACACATGGCATAACGTGACTGAAAGTTCACGAGTGACAACTAAGCATACTTTTGCCACTCTCTCATTTGTGGCCGAGATCTTTATCTTTCTTTATGTCGGCATGGATACCCTGGACATTGACAAGTGGAGATTTGTGAGTGATAGCCCTGGAAAATCCATGGCGGTGAGTTCGATACTGTTGGTACTGGTTCTGGTTGGAAGAGCAGCCTTTGTTTTTCCCTTGTCTTTCTTATCCAACTTGACTAAGAAATCTCCACAAGACAAGCTTAGTTTAAAGCAACAAGTTACAGTTTGGTGGGCGGGGCTTATGCGTGGCTCTGTTTCAATGGCACTTGCTTATAATCAGTTTAATAGGTCTGGCCATACGGACTTGCGTGCGAATGCGATCATGATCACCAGCACCATCACAGTTGTTCTTTTCAGCACGGTGGTGTTTGGTTTGATGACTAAACCACTTGTGAGAATCTTGCTTCCTGTCTCAAAACACGTCTCGAGTATGATTTCTTCTGAACCGTCTAGTCCTAAATCAATCGTTGTGCCACTACTCGGCAATGGCCAAGATTCAGAAGTGAACTGTGGGGTGGAAGATGCAGAGGCAAACATAGGACCCGTGGACATACCCCGTCCAACTAGTTTACGAATGCTCTTAAGCTCTCCTTCTCACACCGTCCACCATTACTGGCGAAGATTTGATAATGCTTTCATGCGGCCTGTTTTTGGCGGGCGGGGTTTTGTGCCTTTCGTTCCAGGCTCACCTGTTGAAGAAATTGGAAATCAATGGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006873 GO:0006885 GO:0006950 GO:0006970 GO:0008150 GO:0008324 GO:0009628 GO:0009651 GO:0009987 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015078 GO:0015079 GO:0015081 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015385 GO:0015386 GO:0015491 GO:0015672 GO:0016020 GO:0019725 GO:0022804 GO:0022821 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030641 GO:0031090 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0090333 GO:0098588 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098719 GO:0098739 GO:0098771 GO:0098805 GO:0099516 GO:0099587 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

552

Amino Acids

60.55

Weight (kDa)

7.26

Isoelectric Point (pI)

41.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	32 - 444	3.2e-58	Sodium/hydrogen exchanger, transmembrane

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05030 AT3G05030 AT3G05030 AT3G05030 AT5G27150
fragaria_vesca	FvH4_4g17120 FvH4_4g17120 FvH4_6g04140
malus_domestica	MD00G1051300.v1.1 MD12G1232400.v1.1 MD13G1174300.v1.1 MD16G1175800.v1.1
prunus_persica	Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.1G142700_v2.0.a1 Prupe.6G335800_v2.0.a1
pyrus_communis	pycom12g21480 pycom13g14990 pycom16g14730
rosa_chinensis	RchiOBHm_Chr3g0452721 RchiOBHm_Chr4g0420901 RchiOBHm_Chr5g0080441
rosa_laevigata	RLG00000007726 RLG00000025572
rosa_multiflora	Rmu_sc0002006.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363300 Rroxscaffold_6G00429730
rosa_rugosa	Rorug02G0642900 Rorug02G0642900 Rorug04G0166900 Rorug04G0167000 Rorug04G0167100
rosa_samantha	Rh3AG045100 Rh3BG047600 Rh3CG046100 Rh3DG046400 Rh4BG231400 Rh4DG226500
rosa_wichuraiana	Rw3G003530 Rw4G019760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1209
AccII	CGCG	2 cut(s)	464, 759
AciI	CCGC	8 cut(s)	5, 275, 849, 1031, 1169, 1576, 1590, 1594
AcoI	YGGCCR	3 cut(s)	939, 1228, 1423
AcsI	RAATTY	1 cut(s)	42
AfaI	GTAC	3 cut(s)	533, 679, 1053
AfiI	CCNNNNNNNGG	3 cut(s)	91, 1415, 1586
AflII	CTTAAG	1 cut(s)	1516
AflIII	ACRYGT	1 cut(s)	1354
AgsI	TTSAA	3 cut(s)	589, 1194, 1631
AhlI	ACTAGT	1 cut(s)	1499
AjiI	CACGTC	1 cut(s)	1357
AjnI	CCWGG	6 cut(s)	300, 537, 684, 978, 1015, 1615
AleI	CACNNNNGTG	2 cut(s)	68, 1298
AluBI	AGCT	6 cut(s)	225, 603, 698, 730, 1135, 1521
AluI	AGCT	6 cut(s)	225, 603, 698, 730, 1135, 1521
Alw21I	GWGCWC	2 cut(s)	20, 188
Alw26I	GTCTC	3 cut(s)	867, 1351, 1363
Alw44I	GTGCAC	1 cut(s)	184
AlwNI	CAGNNNCTG	1 cut(s)	505
Ama87I	CYCGRG	1 cut(s)	1360
AoxI	GGCC	5 cut(s)	51, 939, 1228, 1423, 1577
ApaLI	GTGCAC	1 cut(s)	184
ApeKI	GCWGC	2 cut(s)	695, 1073
ApoI	RAATTY	1 cut(s)	42
Asp700I	GAANNNNTTC	1 cut(s)	86
AspLEI	GCGC	3 cut(s)	97, 107, 464
AspS9I	GGNCC	1 cut(s)	1475
AsuC2I	CCSGG	1 cut(s)	295
AsuHPI	GGTGA	3 cut(s)	1045, 1255, 1614
AvaI	CYCGRG	1 cut(s)	1360
AvaII	GGWCC	1 cut(s)	1475
AxyI	CCTNAGG	1 cut(s)	391
BaeGI	GKGCMC	1 cut(s)	188
BalI	TGGCCA	2 cut(s)	1230, 1425
BarI	GAAGNNNNNNTAC	2 cut(s)	1357, 1389
BauI	CACGAG	1 cut(s)	897
Bbv12I	GWGCWC	2 cut(s)	20, 188
BbvI	GCAGC	2 cut(s)	707, 1085
BccI	CCATC	5 cut(s)	43, 138, 167, 389, 1280
BcgI	CGANNNNNNTGC	2 cut(s)	305, 339
BciT130I	CCWGG	6 cut(s)	302, 539, 686, 980, 1017, 1617
BciVI	GTATCC	2 cut(s)	397, 967
BclI	TGATCA	1 cut(s)	1260
BcnI	CCSGG	1 cut(s)	295
BcoDI	GTCTC	3 cut(s)	867, 1351, 1363
BcuI	ACTAGT	1 cut(s)	1499
BfaI	CTAG	2 cut(s)	1386, 1500
BfoI	RGCGCY	1 cut(s)	98
BfrI	CTTAAG	1 cut(s)	1516
BfuI	GTATCC	2 cut(s)	397, 967
BglII	AGATCT	2 cut(s)	250, 945
BisI	GCNGC	3 cut(s)	696, 1074, 1577
BlsI	GCNGC	3 cut(s)	697, 1075, 1578
Bme1390I	CCNGG	7 cut(s)	295, 302, 539, 686, 980, 1017, 1617
Bme18I	GGWCC	1 cut(s)	1475
BmeT110I	CYCGRG	1 cut(s)	1360
BmgBI	CACGTC	1 cut(s)	1357
BmgT120I	GGNCC	1 cut(s)	1475
BmiI	GGNNCC	3 cut(s)	299, 430, 1477
BmrFI	CCNGG	7 cut(s)	295, 302, 539, 686, 980, 1017, 1617
BmsI	GCATC	2 cut(s)	556, 1447
BpmI	CTGGAG	2 cut(s)	152, 234
BpuEI	CTTGAG	1 cut(s)	419
BpuMI	CCSGG	1 cut(s)	295
BsaAI	YACGTR	1 cut(s)	799
BsaBI	GATNNNNATC	2 cut(s)	950, 1259
BsaJI	CCNNGG	5 cut(s)	685, 978, 1015, 1026, 1479
Bsc4I	CCNNNNNNNGG	3 cut(s)	91, 1415, 1586
Bse1I	ACTGG	3 cut(s)	217, 1059, 1553
Bse21I	CCTNAGG	1 cut(s)	391
Bse3DI	GCAATG	2 cut(s)	405, 1426
Bse8I	GATNNNNATC	2 cut(s)	950, 1259
BseBI	CCWGG	6 cut(s)	302, 539, 686, 980, 1017, 1617
BseDI	CCNNGG	5 cut(s)	685, 978, 1015, 1026, 1479
BseGI	GGATG	2 cut(s)	153, 520
BseJI	GATNNNNATC	2 cut(s)	950, 1259
BseLI	CCNNNNNNNGG	3 cut(s)	91, 1415, 1586
BseMI	GCAATG	2 cut(s)	405, 1426
BseNI	ACTGG	3 cut(s)	217, 1059, 1553
BseSI	GKGCMC	1 cut(s)	188
BseXI	GCAGC	2 cut(s)	707, 1085
Bsh1236I	CGCG	2 cut(s)	464, 759
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	5 cut(s)	53, 941, 1230, 1425, 1579
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	2 cut(s)	20, 188
BsiHKCI	CYCGRG	1 cut(s)	1360
BsiSI	CCGG	1 cut(s)	294
BslI	CCNNNNNNNGG	3 cut(s)	91, 1415, 1586
BsmAI	GTCTC	3 cut(s)	867, 1351, 1363
BsmBI	CGTCTC	1 cut(s)	1363
BsmI	GAATGC	2 cut(s)	1255, 1515
BsnI	GGCC	5 cut(s)	53, 941, 1230, 1425, 1579
BsoBI	CYCGRG	1 cut(s)	1360
Bsp1286I	GDGCHC	2 cut(s)	20, 188
Bsp1407I	TGTACA	1 cut(s)	531
Bsp143I	GATC	5 cut(s)	250, 754, 945, 1254, 1260
Bsp19I	CCATGG	1 cut(s)	1026
Bsp68I	TCGCGA	1 cut(s)	759
BspACI	CCGC	8 cut(s)	5, 275, 849, 1031, 1169, 1576, 1590, 1594
BspANI	GGCC	5 cut(s)	53, 941, 1230, 1425, 1579
BspFNI	CGCG	2 cut(s)	464, 759
BspHI	TCATGA	2 cut(s)	333, 1257
BspLI	GGNNCC	3 cut(s)	299, 430, 1477
BspQI	GCTCTTC	1 cut(s)	1063
BspTI	CTTAAG	1 cut(s)	1516
BsrDI	GCAATG	2 cut(s)	405, 1426
BsrGI	TGTACA	1 cut(s)	531
BsrI	ACTGG	3 cut(s)	217, 1059, 1553
BssECI	CCNNGG	5 cut(s)	685, 978, 1015, 1026, 1479
BssMI	GATC	5 cut(s)	250, 754, 945, 1254, 1260
BssSI	CACGAG	1 cut(s)	897
BssT1I	CCWWGG	1 cut(s)	1026
Bst2BI	CACGAG	1 cut(s)	897
Bst2UI	CCWGG	6 cut(s)	302, 539, 686, 980, 1017, 1617
Bst4CI	ACNGT	9 cut(s)	183, 838, 1047, 1159, 1279, 1297, 1383, 1445, 1537
Bst6I	CTCTTC	2 cut(s)	260, 1063
BstAFI	CTTAAG	1 cut(s)	1516
BstAUI	TGTACA	1 cut(s)	531
BstBAI	YACGTR	1 cut(s)	799
BstC8I	GCNNGC	4 cut(s)	16, 743, 1245, 1592
BstDEI	CTNAG	5 cut(s)	391, 707, 909, 1113, 1136
BstDSI	CCRYGG	2 cut(s)	1026, 1479
BstF5I	GGATG	2 cut(s)	153, 520
BstFNI	CGCG	2 cut(s)	464, 759
BstH2I	RGCGCY	1 cut(s)	98
BstHHI	GCGC	3 cut(s)	97, 107, 464
BstKTI	GATC	5 cut(s)	253, 757, 948, 1257, 1263
BstMAI	GTCTC	3 cut(s)	867, 1351, 1363
BstMBI	GATC	5 cut(s)	250, 754, 945, 1254, 1260
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 461
BstNI	CCWGG	6 cut(s)	302, 539, 686, 980, 1017, 1617
BstSCI	CCNGG	7 cut(s)	293, 300, 537, 684, 978, 1015, 1615
BstSLI	GKGCMC	1 cut(s)	188
BstUI	CGCG	2 cut(s)	464, 759
BstV1I	GCAGC	2 cut(s)	707, 1085
BstX2I	RGATCY	2 cut(s)	250, 945
BstYI	RGATCY	2 cut(s)	250, 945
Bsu36I	CCTNAGG	1 cut(s)	391
BsuI	GTATCC	2 cut(s)	397, 967
BsuRI	GGCC	5 cut(s)	53, 941, 1230, 1425, 1579
BtgI	CCRYGG	2 cut(s)	1026, 1479
BtrI	CACGTC	1 cut(s)	1357
BtsCI	GGATG	2 cut(s)	153, 520
BtsI	GCAGTG	1 cut(s)	630
BtsIMutI	CAGTG	5 cut(s)	75, 250, 427, 630, 834
BtuMI	TCGCGA	1 cut(s)	759
Cac8I	GCNNGC	4 cut(s)	16, 743, 1245, 1592
CaiI	CAGNNNCTG	1 cut(s)	505
CciI	TCATGA	2 cut(s)	333, 1257
CfoI	GCGC	3 cut(s)	97, 107, 464
Cfr13I	GGNCC	1 cut(s)	1475
Csp6I	GTAC	3 cut(s)	532, 678, 1052
CviAII	CATG	7 cut(s)	334, 781, 873, 970, 1027, 1258, 1573
CviQI	GTAC	3 cut(s)	532, 678, 1052
DdeI	CTNAG	5 cut(s)	391, 707, 909, 1113, 1136
DpnI	GATC	5 cut(s)	252, 756, 947, 1256, 1262
DpnII	GATC	5 cut(s)	250, 754, 945, 1254, 1260
DraI	TTTAAA	1 cut(s)	1143
EaeI	YGGCCR	3 cut(s)	939, 1228, 1423
Eam1104I	CTCTTC	2 cut(s)	260, 1063
EarI	CTCTTC	2 cut(s)	260, 1063
EciI	GGCGGA	1 cut(s)	264
Eco130I	CCWWGG	1 cut(s)	1026
Eco47I	GGWCC	1 cut(s)	1475
Eco81I	CCTNAGG	1 cut(s)	391
Eco88I	CYCGRG	1 cut(s)	1360
EcoO109I	RGGNCCY	1 cut(s)	1475
EcoRII	CCWGG	6 cut(s)	300, 537, 684, 978, 1015, 1615
EcoT14I	CCWWGG	1 cut(s)	1026
ErhI	CCWWGG	1 cut(s)	1026
Esp3I	CGTCTC	1 cut(s)	1363
FaeI	CATG	7 cut(s)	337, 784, 876, 973, 1030, 1261, 1576
FatI	CATG	7 cut(s)	333, 780, 872, 969, 1026, 1257, 1572
FauI	CCCGC	3 cut(s)	1162, 1583, 1587
FbaI	TGATCA	1 cut(s)	1260
Fnu4HI	GCNGC	3 cut(s)	696, 1074, 1577
FokI	GGATG	2 cut(s)	160, 527
Fsp4HI	GCNGC	3 cut(s)	696, 1074, 1577
FspBI	CTAG	2 cut(s)	1386, 1500
GlaI	GCGC	3 cut(s)	96, 106, 463
GluI	GCNGC	3 cut(s)	696, 1074, 1577
GsuI	CTGGAG	2 cut(s)	152, 234
HaeII	RGCGCY	1 cut(s)	98
HaeIII	GGCC	5 cut(s)	53, 941, 1230, 1425, 1579
HapII	CCGG	1 cut(s)	294
HhaI	GCGC	3 cut(s)	97, 107, 464
Hin1II	CATG	7 cut(s)	337, 784, 876, 973, 1030, 1261, 1576
Hin6I	GCGC	3 cut(s)	95, 105, 462
HinP1I	GCGC	3 cut(s)	95, 105, 462
HindIII	AAGCTT	1 cut(s)	1133
HinfI	GANTC	8 cut(s)	32, 155, 482, 508, 595, 690, 1332, 1430
HpaII	CCGG	1 cut(s)	294
HphI	GGTGA	3 cut(s)	1045, 1255, 1614
Hpy166II	GTNNAC	6 cut(s)	186, 896, 1441, 1483, 1505, 1540
Hpy188I	TCNGA	6 cut(s)	48, 58, 600, 808, 1378, 1435
Hpy8I	GTNNAC	6 cut(s)	186, 896, 1441, 1483, 1505, 1540
HpyAV	CCTTC	2 cut(s)	545, 1536
HpyCH4III	ACNGT	9 cut(s)	183, 838, 1047, 1159, 1279, 1297, 1383, 1445, 1537
HpyCH4IV	ACGT	4 cut(s)	347, 798, 882, 1356
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 461
HpyF3I	CTNAG	5 cut(s)	391, 707, 909, 1113, 1136
HpySE526I	ACGT	4 cut(s)	347, 798, 882, 1356
Hsp92II	CATG	7 cut(s)	337, 784, 876, 973, 1030, 1261, 1576
HspAI	GCGC	3 cut(s)	95, 105, 462
Ksp22I	TGATCA	1 cut(s)	1260
Kzo9I	GATC	5 cut(s)	250, 754, 945, 1254, 1260
LguI	GCTCTTC	1 cut(s)	1063
Lsp1109I	GCAGC	2 cut(s)	707, 1085
LweI	GCATC	2 cut(s)	556, 1447
MaeI	CTAG	2 cut(s)	1386, 1500
MaeII	ACGT	4 cut(s)	347, 798, 882, 1356
MaeIII	GTNAC	5 cut(s)	343, 670, 883, 901, 1153
MalI	GATC	5 cut(s)	252, 756, 947, 1256, 1262
MboI	GATC	5 cut(s)	250, 754, 945, 1254, 1260
MboII	GAAGA	8 cut(s)	247, 260, 322, 1080, 1365, 1466, 1566, 1643
MfeI	CAATTG	1 cut(s)	21
MflI	RGATCY	2 cut(s)	250, 945
MhlI	GDGCHC	2 cut(s)	20, 188
MlsI	TGGCCA	2 cut(s)	1230, 1425
MluCI	AATT	9 cut(s)	21, 42, 174, 198, 317, 328, 641, 852, 1635
MluNI	TGGCCA	2 cut(s)	1230, 1425
MlyI	GAGTC	2 cut(s)	164, 699
MmeI	TCCRAC	5 cut(s)	81, 340, 1045, 1128, 1520
MnlI	CCTC	6 cut(s)	64, 127, 209, 443, 620, 1456
Mox20I	TGGCCA	2 cut(s)	1230, 1425
MroXI	GAANNNNTTC	1 cut(s)	86
MscI	TGGCCA	2 cut(s)	1230, 1425
MseI	TTAA	5 cut(s)	288, 561, 1142, 1218, 1517
MslI	CAYNNNNRTG	3 cut(s)	68, 1031, 1298
Msp20I	TGGCCA	2 cut(s)	1230, 1425
MspA1I	CMGCKG	1 cut(s)	698
MspCI	CTTAAG	1 cut(s)	1516
MspI	CCGG	1 cut(s)	294
MspR9I	CCNGG	7 cut(s)	295, 302, 539, 686, 980, 1017, 1617
MunI	CAATTG	1 cut(s)	21
Mva1269I	GAATGC	2 cut(s)	1255, 1515
MvaI	CCWGG	6 cut(s)	302, 539, 686, 980, 1017, 1617
MvnI	CGCG	2 cut(s)	464, 759
MwoI	GCNNNNNNNGC	2 cut(s)	11, 461
NciI	CCSGG	1 cut(s)	295
NcoI	CCATGG	1 cut(s)	1026
NdeII	GATC	5 cut(s)	250, 754, 945, 1254, 1260
NlaIII	CATG	7 cut(s)	337, 784, 876, 973, 1030, 1261, 1576
NlaIV	GGNNCC	3 cut(s)	299, 430, 1477
NmeAIII	GCCGAG	2 cut(s)	967, 1395
NmuCI	GTSAC	3 cut(s)	343, 883, 901
NruI	TCGCGA	1 cut(s)	759
OliI	CACNNNNGTG	2 cut(s)	68, 1298
PaeR7I	CTCGAG	1 cut(s)	1360
PagI	TCATGA	2 cut(s)	333, 1257
PciSI	GCTCTTC	1 cut(s)	1063
PctI	GAATGC	2 cut(s)	1255, 1515
PdmI	GAANNNNTTC	1 cut(s)	86
PfeI	GAWTC	6 cut(s)	32, 482, 508, 595, 1332, 1430
PkrI	GCNGC	3 cut(s)	697, 1075, 1578
PleI	GAGTC	2 cut(s)	163, 698
PpsI	GAGTC	2 cut(s)	163, 698
Ppu21I	YACGTR	1 cut(s)	799
PpuMI	RGGWCCY	1 cut(s)	1475
PsiI	TTATAA	1 cut(s)	1209
Psp5II	RGGWCCY	1 cut(s)	1475
Psp6I	CCWGG	6 cut(s)	300, 537, 684, 978, 1015, 1615
PspGI	CCWGG	6 cut(s)	300, 537, 684, 978, 1015, 1615
PspN4I	GGNNCC	3 cut(s)	299, 430, 1477
PspPI	GGNCC	1 cut(s)	1475
PspPPI	RGGWCCY	1 cut(s)	1475
PstNI	CAGNNNCTG	1 cut(s)	505
PsuI	RGATCY	2 cut(s)	250, 945
PvuII	CAGCTG	1 cut(s)	698
RruI	TCGCGA	1 cut(s)	759
RsaI	GTAC	3 cut(s)	533, 679, 1053
RsaNI	GTAC	3 cut(s)	532, 678, 1052
RseI	CAYNNNNRTG	3 cut(s)	68, 1031, 1298
SapI	GCTCTTC	1 cut(s)	1063
SaqAI	TTAA	5 cut(s)	288, 561, 1142, 1218, 1517
SatI	GCNGC	3 cut(s)	696, 1074, 1577
Sau3AI	GATC	5 cut(s)	250, 754, 945, 1254, 1260
Sau96I	GGNCC	1 cut(s)	1475
SchI	GAGTC	2 cut(s)	164, 699
ScrFI	CCNGG	7 cut(s)	295, 302, 539, 686, 980, 1017, 1617
SduI	GDGCHC	2 cut(s)	20, 188
SfaNI	GCATC	2 cut(s)	556, 1447
Sfr274I	CTCGAG	1 cut(s)	1360
SinI	GGWCC	1 cut(s)	1475
SlaI	CTCGAG	1 cut(s)	1360
SmiMI	CAYNNNNRTG	3 cut(s)	68, 1031, 1298
SmlI	CTYRAG	3 cut(s)	434, 1360, 1516
SmoI	CTYRAG	3 cut(s)	434, 1360, 1516
SpeI	ACTAGT	1 cut(s)	1499
Sse9I	AATT	9 cut(s)	21, 42, 174, 198, 317, 328, 641, 852, 1635
SsiI	CCGC	8 cut(s)	5, 275, 849, 1031, 1169, 1576, 1590, 1594
SspMI	CTAG	2 cut(s)	1386, 1500
StyD4I	CCNGG	7 cut(s)	293, 300, 537, 684, 978, 1015, 1615
StyI	CCWWGG	1 cut(s)	1026
TaaI	ACNGT	9 cut(s)	183, 838, 1047, 1159, 1279, 1297, 1383, 1445, 1537
TaiI	ACGT	4 cut(s)	350, 801, 885, 1359
TaqI	TCGA	6 cut(s)	9, 77, 142, 606, 1040, 1361
TasI	AATT	9 cut(s)	21, 42, 174, 198, 317, 328, 641, 852, 1635
TatI	WGTACW	2 cut(s)	531, 677
TauI	GCSGC	1 cut(s)	1579
TfiI	GAWTC	6 cut(s)	32, 482, 508, 595, 1332, 1430
Tru1I	TTAA	5 cut(s)	288, 561, 1142, 1218, 1517
Tru9I	TTAA	5 cut(s)	288, 561, 1142, 1218, 1517
TscAI	CASTG	5 cut(s)	75, 250, 427, 637, 841
TseFI	GTSAC	3 cut(s)	343, 883, 901
TseI	GCWGC	2 cut(s)	695, 1073
Tsp45I	GTSAC	3 cut(s)	343, 883, 901
TspDTI	ATGAA	6 cut(s)	53, 95, 164, 322, 531, 1561
TspGWI	ACGGA	2 cut(s)	44, 1250
TspRI	CASTG	5 cut(s)	75, 250, 427, 637, 841
Vha464I	CTTAAG	1 cut(s)	1516
VneI	GTGCAC	1 cut(s)	184
VpaK11BI	GGWCC	1 cut(s)	1475
XapI	RAATTY	1 cut(s)	42
XhoI	CTCGAG	1 cut(s)	1360
XmnI	GAANNNNTTC	1 cut(s)	86
XspI	CTAG	2 cut(s)	1386, 1500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.