Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001451

Physical Location & Seq

Genomic Coordinates Forward (+)

32215439 .. 32218156

Gene Structure 2718 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1253300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGGCCAGGAAAGGTGTGGTCTCCGACGAGGGAACTGTGACCGACGCCGACAAATCCACGGTGGCGGCGAAGGCGAAGGCTGTGGAGATAAAGAAGCAACTGGAGAGGCTGGTGAAGGCCATTGTCGATGAAGACGACTACAGGGTCGAGACTGCTGACGAGGCCATCAGAGCCTTGTCTTATTTGAAAGACTTGAAGTCCAAAAAGTCTCTCTCTTTCAAGCTTCACGGCGGCGGCGGCGGTACTGTAGTCATCCCTAAAGAATTCAGATGTCCCATTTCCGGAGAGCTCATGGCCGACCCTGTTGTCTTGGCCACTGGAGAGACGTTTGATCGACCATTCATCCAGAGGTGGTTTAACGAAGGCCACAGAACTTGCCCTCAAACCAAGCAAGTCATCTCTCATACTGTCCTTACCCCTAATCACCTGGTTCGAGAAATGGTTTCACAATGGTGCAGGGAGCATGAAATTGAGCTTCCGAAACCTGTTCAGGATATTGATGAGCAACTTGTCACCAATGCAGACCGAGGATGTCTGAATTCACTGCTTGAGAAAATGTCATCATCTCTATCTGATCAAAAAGCAGCAGCAAAACAGCTTCGACTGCTAACAAAGCGAAGTTCATCATTTCGGGCACTTTTTGGTGAGTCTACTGAGACCATTCCGCAATTGCTCAATCCACTTTCACCGGGACAGGTTGATGCTCATCCTGATCTTCAAGAAGATCTGATCACAACACTACTAAACCTCTCAATTCATGACAATAACAAAAGATTAGTCGCAGAGAACGCATCAGTCATCCGTCTGCTTATTGAATCCTTGGAATATGGAACTGTCCAAACAAAAAGCAATGCTGCCGCAACTTTCTTCACATTATCAGCAATTGATTCCAACAAGATTACTATTGGAGCATCCGGTGCTATGAAACCTTTGATTGACCTTTTAGACGTGGGGCATCCATTAGCAATGAAGGATGTTGCTTCGGCAGTATTCAGCCTGTGTACTATCCTCGAAAATAAGGAAAGGGCCATCAATGCTGGAGCAGTCGGGGTGATTCTGATAAAGATAATGGATCATGTTTTAGTTGCCGAGTTTTTAGTTATACTTGCAATGCTTTCCAACCATCAGAGGGCTGTTCAGGAAATGTGTGAGCTTGGCGCTGTGCCTTGCTTGATTGGAATCACAAGGGAGAGCGACTGCGAGCGCAACAAGGAGAACTGTGTTGCAATCCTTTACACTATCTGTTTTGCTGACCGGACCAAACTGAAGGAAATTAAGGAAGAGGAAATGACCAGAGGTACATTTTCTAGACTTGTACAGACCGGAACTTCGAGAGCAAAGAGAAAGGCGAGTGGAATTCTTGAAAGACTGAATAGGACCGGTTCAATAACGCACACAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0032870 GO:0033612 GO:0033993 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050896 GO:0051716 GO:0070647 GO:0070696 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

468

Amino Acids

51.37

Weight (kDa)

6.84

Isoelectric Point (pI)

31.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	85 - 156	3.7e-20	U-box domain
ARM_PUB	PF25598	180 - 352	5.8e-06	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001451)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07360 AT3G07360 AT3G07360 AT3G07360 AT3G07360 AT3G07360 AT3G07360 AT3G07360 AT3G07360 AT3G07360
fragaria_vesca	FvH4_2g18070 FvH4_6g01250
malus_domestica	MD04G1235600.v1.1 MD05G1165400.v1.1 MD10G1154600.v1.1 MD12G1253300.v1.1
prunus_persica	Prupe.6G355500_v2.0.a1 Prupe.6G355500_v2.0.a1 Prupe.6G355500_v2.0.a1 Prupe.6G355500_v2.0.a1 Prupe.6G355500_v2.0.a1 Prupe.8G193500_v2.0.a1 Prupe.8G193500_v2.0.a1 Prupe.8G193500_v2.0.a1 Prupe.8G193500_v2.0.a1 Prupe.8G193500_v2.0.a1
pyrus_communis	pycom04g20910 pycom05g15400 pycom10g09710 pycom10g13370 pycom12g23240
rosa_chinensis	RchiOBHm_Chr3g0448811 RchiOBHm_Chr6g0283461
rosa_laevigata	RLG00000012824 RLG00000025873
rosa_multiflora	Rmu_sc0003116.1_g000024 Rmu_sc0004959.1_g000016
rosa_roxburghii	Rroxscaffold_6G00427030 Rroxscaffold_7G00185520
rosa_rugosa	Rorug02G0616500.1 Rorug02G0616600 Rorug06G0154400
rosa_samantha	Rh3AG014900 Rh3BG014700 Rh3CG013600 Rh3DG015200 Rh6AG266700 Rh6BG269200 Rh6CG269100 Rh6DG262500
rosa_wichuraiana	Rw3G001100 Rw6G023090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	143, 305
AccI	GTMKAC	1 cut(s)	650
AccIII	TCCGGA	1 cut(s)	281
AciI	CCGC	7 cut(s)	65, 231, 234, 237, 240, 665, 858
AclWI	GGATC	1 cut(s)	1080
AcoI	YGGCCR	3 cut(s)	3, 294, 312
AcsI	RAATTY	3 cut(s)	263, 538, 1356
AcuI	CTGAAG	1 cut(s)	1286
AcyI	GRCGYC	1 cut(s)	45
AfaI	GTAC	4 cut(s)	244, 1003, 1300, 1317
AfiI	CCNNNNNNNGG	2 cut(s)	281, 1129
AgeI	ACCGGT	1 cut(s)	1379
AgsI	TTSAA	7 cut(s)	187, 196, 220, 719, 815, 1364, 1386
AhdI	GACNNNNNGTC	1 cut(s)	196
AjiI	CACGTC	1 cut(s)	949
AjnI	CCWGG	2 cut(s)	5, 426
AleI	CACNNNNGTG	1 cut(s)	451
AluBI	AGCT	6 cut(s)	223, 289, 475, 598, 1153, 1400
AluI	AGCT	6 cut(s)	223, 289, 475, 598, 1153, 1400
Alw21I	GWGCWC	1 cut(s)	291
Alw26I	GTCTC	5 cut(s)	25, 143, 213, 317, 650
AlwI	GGATC	1 cut(s)	1080
Aor13HI	TCCGGA	1 cut(s)	281
AoxI	GGCC	7 cut(s)	3, 117, 162, 294, 312, 364, 1026
ApeKI	GCWGC	3 cut(s)	584, 587, 854
ApoI	RAATTY	3 cut(s)	263, 538, 1356
ArsI	GACNNNNNNTTYG	4 cut(s)	762, 794, 1312, 1344
AsiGI	ACCGGT	1 cut(s)	1379
AspLEI	GCGC	2 cut(s)	1160, 1206
AspS9I	GGNCC	3 cut(s)	1026, 1257, 1377
AsuC2I	CCSGG	1 cut(s)	690
AsuHPI	GGTGA	6 cut(s)	124, 416, 505, 656, 678, 1063
AvaII	GGWCC	2 cut(s)	1257, 1377
BaeGI	GKGCMC	1 cut(s)	637
BaeI	ACNNNNGTAYC	2 cut(s)	234, 267
BalI	TGGCCA	2 cut(s)	5, 314
BanII	GRGCYC	1 cut(s)	291
BbsI	GAAGAC	1 cut(s)	138
Bbv12I	GWGCWC	1 cut(s)	291
BbvI	GCAGC	3 cut(s)	596, 599, 841
BccI	CCATC	3 cut(s)	173, 1037, 1131
BceAI	ACGGC	1 cut(s)	244
BciT130I	CCWGG	2 cut(s)	7, 428
BclI	TGATCA	2 cut(s)	574, 729
BcnI	CCSGG	1 cut(s)	690
BcoDI	GTCTC	5 cut(s)	25, 143, 213, 317, 650
BfaI	CTAG	1 cut(s)	1308
BfmI	CTRYAG	2 cut(s)	139, 246
BfoI	RGCGCY	1 cut(s)	1161
BglII	AGATCT	1 cut(s)	724
BisI	GCNGC	8 cut(s)	66, 232, 235, 238, 585, 588, 855, 858
BlsI	GCNGC	8 cut(s)	67, 233, 236, 239, 586, 589, 856, 859
Bme1390I	CCNGG	3 cut(s)	7, 428, 690
Bme18I	GGWCC	2 cut(s)	1257, 1377
BmeRI	GACNNNNNGTC	1 cut(s)	196
BmgBI	CACGTC	1 cut(s)	949
BmgT120I	GGNCC	3 cut(s)	1026, 1257, 1377
BmrFI	CCNGG	3 cut(s)	7, 428, 690
BmsI	GCATC	4 cut(s)	691, 800, 920, 964
BpiI	GAAGAC	1 cut(s)	138
BpmI	CTGGAG	3 cut(s)	122, 339, 1059
BpuEI	CTTGAG	1 cut(s)	569
BpuMI	CCSGG	1 cut(s)	690
BsaBI	GATNNNNATC	2 cut(s)	705, 1178
BsaHI	GRCGYC	1 cut(s)	45
BsaI	GGTCTC	2 cut(s)	25, 650
BsaJI	CCNNGG	3 cut(s)	57, 526, 819
BsaWI	WCCGGW	5 cut(s)	281, 914, 1254, 1322, 1379
Bsc4I	CCNNNNNNNGG	2 cut(s)	281, 1129
Bse118I	RCCGGY	1 cut(s)	1379
Bse1I	ACTGG	2 cut(s)	105, 322
Bse3DI	GCAATG	3 cut(s)	856, 972, 1116
Bse8I	GATNNNNATC	2 cut(s)	705, 1178
BseAI	TCCGGA	1 cut(s)	281
BseBI	CCWGG	2 cut(s)	7, 428
BseDI	CCNNGG	3 cut(s)	57, 526, 819
BseGI	GGATG	8 cut(s)	252, 342, 536, 706, 798, 911, 955, 979
BseJI	GATNNNNATC	2 cut(s)	705, 1178
BseLI	CCNNNNNNNGG	2 cut(s)	281, 1129
BseMI	GCAATG	3 cut(s)	856, 972, 1116
BseMII	CTCAG	1 cut(s)	645
BseNI	ACTGG	2 cut(s)	105, 322
BseSI	GKGCMC	1 cut(s)	637
BseXI	GCAGC	3 cut(s)	596, 599, 841
BsgI	GTGCAG	1 cut(s)	475
BshFI	GGCC	7 cut(s)	5, 119, 164, 296, 314, 366, 1028
BshTI	ACCGGT	1 cut(s)	1379
BsiHKAI	GWGCWC	1 cut(s)	291
BsiSI	CCGG	6 cut(s)	282, 689, 915, 1255, 1323, 1380
BslFI	GGGAC	2 cut(s)	258, 705
BslI	CCNNNNNNNGG	2 cut(s)	281, 1129
BsmAI	GTCTC	5 cut(s)	25, 143, 213, 317, 650
BsmBI	CGTCTC	1 cut(s)	317
BsmFI	GGGAC	2 cut(s)	258, 705
BsnI	GGCC	7 cut(s)	5, 119, 164, 296, 314, 366, 1028
Bso31I	GGTCTC	2 cut(s)	25, 650
Bsp1286I	GDGCHC	2 cut(s)	291, 637
Bsp13I	TCCGGA	1 cut(s)	281
Bsp1407I	TGTACA	1 cut(s)	1315
Bsp143I	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
BspACI	CCGC	7 cut(s)	65, 231, 234, 237, 240, 665, 858
BspANI	GGCC	7 cut(s)	5, 119, 164, 296, 314, 366, 1028
BspCNI	CTCAG	1 cut(s)	646
BspEI	TCCGGA	1 cut(s)	281
BspHI	TCATGA	1 cut(s)	757
BspPI	GGATC	1 cut(s)	1080
BspTNI	GGTCTC	2 cut(s)	25, 650
BsrDI	GCAATG	3 cut(s)	856, 972, 1116
BsrFI	RCCGGY	1 cut(s)	1379
BsrGI	TGTACA	1 cut(s)	1315
BsrI	ACTGG	2 cut(s)	105, 322
BssAI	RCCGGY	1 cut(s)	1379
BssECI	CCNNGG	3 cut(s)	57, 526, 819
BssMI	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
BssNI	GRCGYC	1 cut(s)	45
BssT1I	CCWWGG	1 cut(s)	819
Bst2UI	CCWGG	2 cut(s)	7, 428
Bst4CI	ACNGT	6 cut(s)	37, 61, 247, 409, 835, 1220
Bst6I	CTCTTC	1 cut(s)	1275
BstACI	GRCGYC	1 cut(s)	45
BstAPI	GCANNNNNTGC	1 cut(s)	917
BstAUI	TGTACA	1 cut(s)	1315
BstC8I	GCNNGC	1 cut(s)	1202
BstDEI	CTNAG	1 cut(s)	654
BstDSI	CCRYGG	1 cut(s)	57
BstF5I	GGATG	8 cut(s)	252, 342, 536, 706, 798, 911, 955, 979
BstH2I	RGCGCY	1 cut(s)	1161
BstHHI	GCGC	2 cut(s)	1160, 1206
BstKTI	GATC	6 cut(s)	334, 577, 715, 727, 732, 1075
BstMAI	GTCTC	5 cut(s)	25, 143, 213, 317, 650
BstMBI	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
BstMWI	GCNNNNNNNGC	9 cut(s)	71, 161, 170, 237, 604, 613, 788, 917, 1034
BstNI	CCWGG	2 cut(s)	7, 428
BstSCI	CCNGG	3 cut(s)	5, 426, 688
BstSFI	CTRYAG	2 cut(s)	139, 246
BstSLI	GKGCMC	1 cut(s)	637
BstV1I	GCAGC	3 cut(s)	596, 599, 841
BstV2I	GAAGAC	1 cut(s)	138
BstX2I	RGATCY	1 cut(s)	724
BstYI	RGATCY	1 cut(s)	724
BsuRI	GGCC	7 cut(s)	5, 119, 164, 296, 314, 366, 1028
BtgI	CCRYGG	1 cut(s)	57
BtrI	CACGTC	1 cut(s)	949
BtsCI	GGATG	8 cut(s)	252, 342, 536, 706, 798, 911, 955, 979
BtsI	GCAGTG	1 cut(s)	542
BtsIMutI	CAGTG	2 cut(s)	315, 542
Cac8I	GCNNGC	1 cut(s)	1202
CciI	TCATGA	1 cut(s)	757
CfoI	GCGC	2 cut(s)	1160, 1206
Cfr10I	RCCGGY	1 cut(s)	1379
Cfr13I	GGNCC	3 cut(s)	1026, 1257, 1377
CseI	GACGC	1 cut(s)	53
CsiI	ACCWGGT	1 cut(s)	426
Csp6I	GTAC	4 cut(s)	243, 1002, 1299, 1316
CspAI	ACCGGT	1 cut(s)	1379
CspCI	CAANNNNNGTGG	2 cut(s)	356, 391
CviAII	CATG	4 cut(s)	292, 464, 758, 1076
CviQI	GTAC	4 cut(s)	243, 1002, 1299, 1316
DdeI	CTNAG	1 cut(s)	654
DpnI	GATC	6 cut(s)	333, 576, 714, 726, 731, 1074
DpnII	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
DrdI	GACNNNNNNGTC	2 cut(s)	143, 305
DriI	GACNNNNNGTC	1 cut(s)	196
DseDI	GACNNNNNNGTC	2 cut(s)	143, 305
EaeI	YGGCCR	3 cut(s)	3, 294, 312
Eam1104I	CTCTTC	1 cut(s)	1275
Eam1105I	GACNNNNNGTC	1 cut(s)	196
EarI	CTCTTC	1 cut(s)	1275
Ecl136II	GAGCTC	1 cut(s)	289
Eco130I	CCWWGG	1 cut(s)	819
Eco24I	GRGCYC	1 cut(s)	291
Eco31I	GGTCTC	2 cut(s)	25, 650
Eco47I	GGWCC	2 cut(s)	1257, 1377
Eco53kI	GAGCTC	1 cut(s)	289
Eco57I	CTGAAG	1 cut(s)	1286
EcoICRI	GAGCTC	1 cut(s)	289
EcoRI	GAATTC	3 cut(s)	263, 538, 1356
EcoRII	CCWGG	2 cut(s)	5, 426
EcoT14I	CCWWGG	1 cut(s)	819
EcoT38I	GRGCYC	1 cut(s)	291
ErhI	CCWWGG	1 cut(s)	819
Esp3I	CGTCTC	1 cut(s)	317
FaeI	CATG	4 cut(s)	295, 467, 761, 1079
FaiI	YATR	8 cut(s)	293, 405, 465, 759, 828, 923, 1077, 1103
FaqI	GGGAC	2 cut(s)	258, 705
FatI	CATG	4 cut(s)	291, 463, 757, 1075
FbaI	TGATCA	2 cut(s)	574, 729
FblI	GTMKAC	1 cut(s)	650
Fnu4HI	GCNGC	8 cut(s)	66, 232, 235, 238, 585, 588, 855, 858
FokI	GGATG	8 cut(s)	239, 329, 543, 693, 785, 898, 942, 986
FriOI	GRGCYC	1 cut(s)	291
Fsp4HI	GCNGC	8 cut(s)	66, 232, 235, 238, 585, 588, 855, 858
FspBI	CTAG	1 cut(s)	1308
GlaI	GCGC	2 cut(s)	1159, 1205
GluI	GCNGC	8 cut(s)	66, 232, 235, 238, 585, 588, 855, 858
GsuI	CTGGAG	3 cut(s)	122, 339, 1059
HaeII	RGCGCY	1 cut(s)	1161
HaeIII	GGCC	7 cut(s)	5, 119, 164, 296, 314, 366, 1028
HapII	CCGG	6 cut(s)	282, 689, 915, 1255, 1323, 1380
HgaI	GACGC	1 cut(s)	53
HhaI	GCGC	2 cut(s)	1160, 1206
Hin1I	GRCGYC	1 cut(s)	45
Hin1II	CATG	4 cut(s)	295, 467, 761, 1079
Hin6I	GCGC	2 cut(s)	1158, 1204
HinP1I	GCGC	2 cut(s)	1158, 1204
HindIII	AAGCTT	1 cut(s)	221
HinfI	GANTC	5 cut(s)	647, 815, 887, 1054, 1179
HpaII	CCGG	6 cut(s)	282, 689, 915, 1255, 1323, 1380
HphI	GGTGA	6 cut(s)	124, 416, 505, 656, 678, 1063
Hpy166II	GTNNAC	2 cut(s)	651, 1002
Hpy188I	TCNGA	9 cut(s)	25, 170, 269, 480, 537, 574, 729, 1059, 1128
Hpy8I	GTNNAC	2 cut(s)	651, 1002
Hpy99I	CGWCG	2 cut(s)	29, 47
HpyAV	CCTTC	6 cut(s)	64, 70, 109, 356, 964, 1261
HpyCH4III	ACNGT	6 cut(s)	37, 61, 247, 409, 835, 1220
HpyCH4IV	ACGT	2 cut(s)	326, 948
HpyCH4V	TGCA	4 cut(s)	456, 521, 1109, 1226
HpyF10VI	GCNNNNNNNGC	9 cut(s)	71, 161, 170, 237, 604, 613, 788, 917, 1034
HpyF3I	CTNAG	1 cut(s)	654
HpySE526I	ACGT	2 cut(s)	326, 948
Hsp92I	GRCGYC	1 cut(s)	45
Hsp92II	CATG	4 cut(s)	295, 467, 761, 1079
HspAI	GCGC	2 cut(s)	1158, 1204
Kpn2I	TCCGGA	1 cut(s)	281
Ksp22I	TGATCA	2 cut(s)	574, 729
Kzo9I	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
LmnI	GCTCC	3 cut(s)	460, 908, 1040
Lsp1109I	GCAGC	3 cut(s)	596, 599, 841
LweI	GCATC	4 cut(s)	691, 800, 920, 964
MabI	ACCWGGT	1 cut(s)	426
MaeI	CTAG	1 cut(s)	1308
MaeII	ACGT	2 cut(s)	326, 948
MaeIII	GTNAC	2 cut(s)	37, 511
MalI	GATC	6 cut(s)	333, 576, 714, 726, 731, 1074
MboI	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
MboII	GAAGA	5 cut(s)	143, 707, 734, 859, 1292
MfeI	CAATTG	2 cut(s)	668, 882
MflI	RGATCY	1 cut(s)	724
MhlI	GDGCHC	2 cut(s)	291, 637
MlsI	TGGCCA	2 cut(s)	5, 314
MluCI	AATT	8 cut(s)	263, 468, 538, 668, 753, 882, 1272, 1356
MluNI	TGGCCA	2 cut(s)	5, 314
MlyI	GAGTC	1 cut(s)	656
MmeI	TCCRAC	3 cut(s)	48, 915, 1143
Mox20I	TGGCCA	2 cut(s)	5, 314
MroI	TCCGGA	1 cut(s)	281
MscI	TGGCCA	2 cut(s)	5, 314
MseI	TTAA	2 cut(s)	357, 1275
MslI	CAYNNNNRTG	1 cut(s)	451
Msp20I	TGGCCA	2 cut(s)	5, 314
MspI	CCGG	6 cut(s)	282, 689, 915, 1255, 1323, 1380
MspR9I	CCNGG	3 cut(s)	7, 428, 690
MunI	CAATTG	2 cut(s)	668, 882
MvaI	CCWGG	2 cut(s)	7, 428
MwoI	GCNNNNNNNGC	9 cut(s)	71, 161, 170, 237, 604, 613, 788, 917, 1034
NciI	CCSGG	1 cut(s)	690
NdeII	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
NlaIII	CATG	4 cut(s)	295, 467, 761, 1079
NmeAIII	GCCGAG	1 cut(s)	1114
NmuCI	GTSAC	2 cut(s)	37, 511
OliI	CACNNNNGTG	1 cut(s)	451
PagI	TCATGA	1 cut(s)	757
PcsI	WCGNNNNNNNCGW	2 cut(s)	65, 132
PfeI	GAWTC	4 cut(s)	815, 887, 1054, 1179
PinAI	ACCGGT	1 cut(s)	1379
PkrI	GCNGC	8 cut(s)	67, 233, 236, 239, 586, 589, 856, 859
PleI	GAGTC	1 cut(s)	655
PpsI	GAGTC	1 cut(s)	655
Psp124BI	GAGCTC	1 cut(s)	291
Psp6I	CCWGG	2 cut(s)	5, 426
PspGI	CCWGG	2 cut(s)	5, 426
PspPI	GGNCC	3 cut(s)	1026, 1257, 1377
PsuI	RGATCY	1 cut(s)	724
RsaI	GTAC	4 cut(s)	244, 1003, 1300, 1317
RsaNI	GTAC	4 cut(s)	243, 1002, 1299, 1316
RseI	CAYNNNNRTG	1 cut(s)	451
SacI	GAGCTC	1 cut(s)	291
SaqAI	TTAA	2 cut(s)	357, 1275
SatI	GCNGC	8 cut(s)	66, 232, 235, 238, 585, 588, 855, 858
Sau3AI	GATC	6 cut(s)	331, 574, 712, 724, 729, 1072
Sau96I	GGNCC	3 cut(s)	1026, 1257, 1377
SchI	GAGTC	1 cut(s)	656
ScrFI	CCNGG	3 cut(s)	7, 428, 690
SduI	GDGCHC	2 cut(s)	291, 637
SexAI	ACCWGGT	1 cut(s)	426
SfaNI	GCATC	4 cut(s)	691, 800, 920, 964
SfcI	CTRYAG	2 cut(s)	139, 246
SinI	GGWCC	2 cut(s)	1257, 1377
SmiMI	CAYNNNNRTG	1 cut(s)	451
SmlI	CTYRAG	1 cut(s)	548
SmoI	CTYRAG	1 cut(s)	548
Sse9I	AATT	8 cut(s)	263, 468, 538, 668, 753, 882, 1272, 1356
SsiI	CCGC	7 cut(s)	65, 231, 234, 237, 240, 665, 858
SspMI	CTAG	1 cut(s)	1308
SstI	GAGCTC	1 cut(s)	291
StyD4I	CCNGG	3 cut(s)	5, 426, 688
StyI	CCWWGG	1 cut(s)	819
TaaI	ACNGT	6 cut(s)	37, 61, 247, 409, 835, 1220
TaiI	ACGT	2 cut(s)	329, 951
TaqI	TCGA	7 cut(s)	126, 147, 334, 433, 601, 1011, 1331
TaqII	GACCGA	2 cut(s)	56, 540
TasI	AATT	8 cut(s)	263, 468, 538, 668, 753, 882, 1272, 1356
TatI	WGTACW	2 cut(s)	1001, 1315
TauI	GCSGC	5 cut(s)	68, 234, 237, 240, 860
TfiI	GAWTC	4 cut(s)	815, 887, 1054, 1179
Tru1I	TTAA	2 cut(s)	357, 1275
Tru9I	TTAA	2 cut(s)	357, 1275
TscAI	CASTG	2 cut(s)	322, 549
TseFI	GTSAC	2 cut(s)	37, 511
TseI	GCWGC	3 cut(s)	584, 587, 854
Tsp45I	GTSAC	2 cut(s)	37, 511
TspDTI	ATGAA	7 cut(s)	144, 331, 480, 612, 746, 938, 983
TspGWI	ACGGA	1 cut(s)	791
TspRI	CASTG	2 cut(s)	322, 549
VpaK11BI	GGWCC	2 cut(s)	1257, 1377
XapI	RAATTY	3 cut(s)	263, 538, 1356
XbaI	TCTAGA	1 cut(s)	1307
XcmI	CCANNNNNNNNNTGG	1 cut(s)	13
XmiI	GTMKAC	1 cut(s)	650
XspI	CTAG	1 cut(s)	1308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.