MD13G1027500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009102
Physical Location & Seq
Forward (+)
1975453 .. 1975920
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1027500.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGTAGCAACTTCACTCATTTCTTCTTCTTCTTCTTCTTACTCCAACGTCTTTTCGTTCCACCCGATTTCCGGCACAACCCGAAACCCAAACCGGAACGTTTCTTCAACTAGGGTTTTTGCATCGAGTAGGAAAGGAGCCGATCATGCAGACAAGTACTACCAAAACTACAGCGGCAGGCTGGTGGACGAGAACATGATTGTTCTCCGGAAGAGAGTTCACGAGATGAAGATGGTGGAGAGGAACTACGAGCCTCCGTCGGATTGGATGGACTGGGAGAAGCGATATTACACCACCTACGATTCGTTTATATGCCAAGTCATGGGCCTCTTGCAGTCCCAGTTGATGAACACCAGGCCGAGTTTTGCTTTGGCATTCTTGGCTTTGATCGTTTTGAGCGTGCCTACTTCAACGTTTACGGTTTTCTTCCATTTTTTGGAGCTCGCAAAGGGGGTCGGTCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.76

Weight (kDa)

9.3

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 322, 436
AccIII TCCGGA 1 cut(s) 207
AciI CCGC 1 cut(s) 174
AclI AACGTT 2 cut(s) 99, 413
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 71, 322, 436
AgsI TTSAA 2 cut(s) 108, 411
AjnI CCWGG 1 cut(s) 353
AluBI AGCT 1 cut(s) 442
AluI AGCT 1 cut(s) 442
Alw21I GWGCWC 1 cut(s) 444
Aor13HI TCCGGA 1 cut(s) 207
AoxI GGCC 2 cut(s) 325, 356
AspS9I GGNCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 444
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
BauI CACGAG 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 444
BccI CCATC 2 cut(s) 226, 262
BciT130I CCWGG 1 cut(s) 355
BfaI CTAG 2 cut(s) 111, 466
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
BmcAI AGTACT 1 cut(s) 158
Bme1390I CCNGG 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 355
BmrI ACTGGG 2 cut(s) 283, 334
BmsI GCATC 1 cut(s) 131
BmuI ACTGGG 2 cut(s) 283, 334
BpiI GAAGAC 1 cut(s) 452
BsaWI WCCGGW 2 cut(s) 93, 207
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 322, 436
Bse1I ACTGG 2 cut(s) 278, 340
BseAI TCCGGA 1 cut(s) 207
BseBI CCWGG 1 cut(s) 355
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 71, 322, 436
BseNI ACTGG 2 cut(s) 278, 340
BshFI GGCC 2 cut(s) 327, 358
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 3 cut(s) 72, 94, 208
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 3 cut(s) 71, 322, 436
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 2 cut(s) 327, 358
Bsp1286I GDGCHC 1 cut(s) 444
Bsp13I TCCGGA 1 cut(s) 207
Bsp143I GATC 2 cut(s) 142, 387
BspACI CCGC 1 cut(s) 174
BspANI GGCC 2 cut(s) 327, 358
BspEI TCCGGA 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 139
BsrI ACTGG 2 cut(s) 278, 340
BssMI GATC 2 cut(s) 142, 387
BssSI CACGAG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 1 cut(s) 421
Bst6I CTCTTC 1 cut(s) 206
BstC8I GCNNGC 3 cut(s) 179, 401, 444
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 2 cut(s) 145, 390
BstMBI GATC 2 cut(s) 142, 387
BstMWI GCNNNNNNNGC 2 cut(s) 146, 380
BstNI CCWGG 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 452
BsuRI GGCC 2 cut(s) 327, 358
BtsCI GGATG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 179, 401, 444
Cfr13I GGNCC 1 cut(s) 325
Csp6I GTAC 1 cut(s) 157
CviAII CATG 3 cut(s) 146, 196, 322
CviJI RGCY 7 cut(s) 140, 181, 253, 327, 358, 383, 442
CviKI_1 RGCY 7 cut(s) 140, 181, 253, 327, 358, 383, 442
CviQI GTAC 1 cut(s) 157
DpnI GATC 2 cut(s) 144, 389
DpnII GATC 2 cut(s) 142, 387
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
Ecl136II GAGCTC 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 444
Eco53kI GAGCTC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 444
FaeI CATG 3 cut(s) 149, 199, 325
FaiI YATR 5 cut(s) 147, 197, 311, 313, 323
FaqI GGGAC 1 cut(s) 322
FatI CATG 3 cut(s) 145, 195, 321
Fnu4HI GCNGC 1 cut(s) 175
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 175
FspBI CTAG 2 cut(s) 111, 466
GluI GCNGC 1 cut(s) 175
HaeIII GGCC 2 cut(s) 327, 358
HapII CCGG 3 cut(s) 72, 94, 208
Hin1II CATG 3 cut(s) 149, 199, 325
HinfI GANTC 1 cut(s) 302
HpaII CCGG 3 cut(s) 72, 94, 208
Hpy166II GTNNAC 3 cut(s) 187, 220, 417
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 208, 221
Hpy8I GTNNAC 3 cut(s) 187, 220, 417
Hpy99I CGWCG 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 3 cut(s) 48, 99, 413
HpyCH4V TGCA 3 cut(s) 122, 149, 334
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 380
HpySE526I ACGT 3 cut(s) 48, 99, 413
Hsp92II CATG 3 cut(s) 149, 199, 325
Kpn2I TCCGGA 1 cut(s) 207
Kzo9I GATC 2 cut(s) 142, 387
LmnI GCTCC 2 cut(s) 137, 439
LpnPI CCDG 9 cut(s) 85, 107, 163, 167, 221, 259, 340, 353, 367
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 111, 466
MaeII ACGT 3 cut(s) 48, 99, 413
MalI GATC 2 cut(s) 144, 389
MboI GATC 2 cut(s) 142, 387
MhlI GDGCHC 1 cut(s) 444
MmeI TCCRAC 2 cut(s) 69, 240
MnlI CCTC 3 cut(s) 234, 264, 338
MroI TCCGGA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 3 cut(s) 72, 94, 208
MspR9I CCNGG 1 cut(s) 355
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 1 cut(s) 355
MwoI GCNNNNNNNGC 2 cut(s) 146, 380
NdeII GATC 2 cut(s) 142, 387
NlaIII CATG 3 cut(s) 149, 199, 325
NlaIV GGNNCC 1 cut(s) 139
NmeAIII GCCGAG 1 cut(s) 384
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 396
PctI GAATGC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 302
PflMI CCANNNNNTGG 2 cut(s) 322, 436
PkrI GCNGC 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 444
Psp1406I AACGTT 2 cut(s) 99, 413
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 1 cut(s) 325
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 444
SatI GCNGC 1 cut(s) 175
Sau3AI GATC 2 cut(s) 142, 387
Sau96I GGNCC 1 cut(s) 325
ScaI AGTACT 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 1 cut(s) 444
SetI ASST 5 cut(s) 51, 102, 299, 416, 444
SfaNI GCATC 1 cut(s) 131
SfcI CTRYAG 1 cut(s) 169
SsiI CCGC 1 cut(s) 174
SspMI CTAG 2 cut(s) 111, 466
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 353
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 3 cut(s) 51, 102, 416
TaqI TCGA 1 cut(s) 125
TaqII GACCGA 1 cut(s) 446
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 1 cut(s) 302
TspDTI ATGAA 2 cut(s) 242, 362
TspGWI ACGGA 1 cut(s) 246
Van91I CCANNNNNTGG 2 cut(s) 322, 436
XspI CTAG 2 cut(s) 111, 466
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.