MD16G1030600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009102
Physical Location & Seq
Forward (+)
2187887 .. 2188348
462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1030600.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGCAGCAATTTCACTCATTTCTTCTTCTTCTTGTTCCAACATTCTTTCATTTAATCCGATTTCCAGCACGACCCGAAACCAAATCCGGAAGGTTTCTTCAACTAGGGTTTTTGCGTCGAGCAGAAAAGGAGGTGATCATGCAGACCAGTACTACCAAAACTACAGCAGCAGGCTGGTGGACGAGAGCATGATTGTTCTCCGGAAGAGAGTTCACGAAATGAAGATGGTGGAGAGAAACTACGAGCCTCCGTCGGATTGGATGGATTGGGAGAAGCGATATTACACCACCTACGATTCGTTTATATGCCAAGTCATGGGCTTCTTGCAGTCCCACTTGATGGACACAAGGCCAAGTTTTGCTTTGGCATTCCTGGCTTTGATCGTTTTGAGCGTGCCTACTTCAACGCTTATGATTTTCTTCCAACTGTTGGAGCTCGCAAAGGGGGTCGGTCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.54

Weight (kDa)

8.95

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 316, 340, 430
AccIII TCCGGA 2 cut(s) 87, 201
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 3 cut(s) 316, 340, 430
AgsI TTSAA 2 cut(s) 102, 405
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 1 cut(s) 436
AluI AGCT 1 cut(s) 436
Alw21I GWGCWC 1 cut(s) 438
Aor13HI TCCGGA 2 cut(s) 87, 201
AoxI GGCC 1 cut(s) 350
ApeKI GCWGC 2 cut(s) 5, 168
AsuHPI GGTGA 1 cut(s) 146
BanII GRGCYC 1 cut(s) 438
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 17, 180
BccI CCATC 3 cut(s) 220, 256, 334
BciT130I CCWGG 1 cut(s) 374
BclI TGATCA 1 cut(s) 136
BfaI CTAG 2 cut(s) 105, 460
BfmI CTRYAG 1 cut(s) 163
BisI GCNGC 2 cut(s) 6, 169
BlsI GCNGC 2 cut(s) 7, 170
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 446
BsaWI WCCGGW 2 cut(s) 87, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 340, 430
Bse1I ACTGG 1 cut(s) 148
BseAI TCCGGA 2 cut(s) 87, 201
BseBI CCWGG 1 cut(s) 374
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 316, 340, 430
BseNI ACTGG 1 cut(s) 148
BseXI GCAGC 2 cut(s) 17, 180
BshFI GGCC 1 cut(s) 352
BsiHKAI GWGCWC 1 cut(s) 438
BsiSI CCGG 2 cut(s) 88, 202
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 3 cut(s) 316, 340, 430
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 438
Bsp13I TCCGGA 2 cut(s) 87, 201
Bsp143I GATC 2 cut(s) 136, 381
BspANI GGCC 1 cut(s) 352
BspEI TCCGGA 2 cut(s) 87, 201
BsrI ACTGG 1 cut(s) 148
BssMI GATC 2 cut(s) 136, 381
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 429
Bst6I CTCTTC 1 cut(s) 200
BstC8I GCNNGC 3 cut(s) 173, 395, 438
BstF5I GGATG 1 cut(s) 267
BstKTI GATC 2 cut(s) 139, 384
BstMBI GATC 2 cut(s) 136, 381
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 17, 180
BstV2I GAAGAC 1 cut(s) 446
BsuRI GGCC 1 cut(s) 352
BtsCI GGATG 1 cut(s) 267
Cac8I GCNNGC 3 cut(s) 173, 395, 438
CseI GACGC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 151
CviAII CATG 3 cut(s) 140, 190, 316
CviJI RGCY 6 cut(s) 175, 247, 321, 352, 377, 436
CviKI_1 RGCY 6 cut(s) 175, 247, 321, 352, 377, 436
CviQI GTAC 1 cut(s) 151
DpnI GATC 2 cut(s) 138, 383
DpnII GATC 2 cut(s) 136, 381
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 438
Eco53kI GAGCTC 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 438
FaeI CATG 3 cut(s) 143, 193, 319
FaiI YATR 6 cut(s) 141, 191, 305, 307, 317, 413
FalI AAGNNNNNCTT 2 cut(s) 346, 378
FaqI GGGAC 1 cut(s) 316
FatI CATG 3 cut(s) 139, 189, 315
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 2 cut(s) 6, 169
FokI GGATG 1 cut(s) 274
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 2 cut(s) 6, 169
FspBI CTAG 2 cut(s) 105, 460
GluI GCNGC 2 cut(s) 6, 169
HaeIII GGCC 1 cut(s) 352
HapII CCGG 2 cut(s) 88, 202
HgaI GACGC 1 cut(s) 105
Hin1II CATG 3 cut(s) 143, 193, 319
HinfI GANTC 1 cut(s) 296
HpaII CCGG 2 cut(s) 88, 202
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 181, 214
Hpy188I TCNGA 2 cut(s) 60, 256
Hpy188III TCNNGA 3 cut(s) 88, 202, 215
Hpy8I GTNNAC 2 cut(s) 181, 214
Hpy99I CGWCG 2 cut(s) 121, 256
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 429
HpyCH4V TGCA 2 cut(s) 143, 328
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
Hsp92II CATG 3 cut(s) 143, 193, 319
Kpn2I TCCGGA 2 cut(s) 87, 201
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 381
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 8 cut(s) 79, 101, 157, 161, 161, 215, 359, 386
Lsp1109I GCAGC 2 cut(s) 17, 180
MaeI CTAG 2 cut(s) 105, 460
MalI GATC 2 cut(s) 138, 383
MboI GATC 2 cut(s) 136, 381
MboII GAAGA 8 cut(s) 15, 18, 21, 90, 217, 235, 412, 446
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 1 cut(s) 9
MmeI TCCRAC 4 cut(s) 63, 234, 411, 448
MnlI CCTC 2 cut(s) 125, 258
MroI TCCGGA 2 cut(s) 87, 201
MseI TTAA 1 cut(s) 54
MspI CCGG 2 cut(s) 88, 202
MspR9I CCNGG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 374
NdeII GATC 2 cut(s) 136, 381
NlaIII CATG 3 cut(s) 143, 193, 319
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 296
PflMI CCANNNNNTGG 3 cut(s) 316, 340, 430
PkrI GCNGC 2 cut(s) 7, 170
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 2 cut(s) 6, 169
Sau3AI GATC 2 cut(s) 136, 381
ScaI AGTACT 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 374
SduI GDGCHC 1 cut(s) 438
SetI ASST 4 cut(s) 96, 136, 293, 438
SfcI CTRYAG 1 cut(s) 163
Sse9I AATT 1 cut(s) 9
SspMI CTAG 2 cut(s) 105, 460
SstI GAGCTC 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 119
TaqII GACCGA 1 cut(s) 440
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TseI GCWGC 2 cut(s) 5, 168
TspDTI ATGAA 2 cut(s) 39, 236
TspGWI ACGGA 1 cut(s) 240
Van91I CCANNNNNTGG 3 cut(s) 316, 340, 430
XspI CTAG 2 cut(s) 105, 460
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.