pycom13g02620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009102
Physical Location & Seq
Forward (+)
1813574 .. 1814038
465 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02620.1

Sequence Viewer

Length: 465 bp
ATGGTAGCAACTTCACTGCTTTCCTCTTCTTCTTCTTACTCCAACGTCTTTTCGTTCCACCCGATTTCCGGCACAACCCGAAACCCAAACCGGAACGTTTCTTCAACTAGGGTTTTTGCATCGAGTAGAAAAGGAGCTGATCATGCAGACAAGTACTACCAAAACTACAGCAGCAGGCTGGTGGACGAGAACATGATTGTTCTCCGGAAGAGAGTTCACGAGATGAAGATGGTGGAGAGGAACTACGAGCCTCCGTCGGATTGGATGGACTGGGAGAATCGATATTACACCACCTACGATTCGTTTATATGCCAAGTCATGGGCCTCTTGCAGTCCCAGTTGATGAATACCAGGCCGAGTTTTGCTTTGGCATTCTTGGCTTTGATCGTTTTGAGCGTGCCTACTTCAACGCTTACGGTTTTCTTCCATTTTTTGGAGCTCGCAAAGGGGGTTGATCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.71

Weight (kDa)

8.74

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 319, 433
AccIII TCCGGA 1 cut(s) 204
AclI AACGTT 1 cut(s) 96
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 3 cut(s) 68, 319, 433
AgsI TTSAA 2 cut(s) 105, 408
AjnI CCWGG 1 cut(s) 350
AluBI AGCT 2 cut(s) 137, 439
AluI AGCT 2 cut(s) 137, 439
Alw21I GWGCWC 1 cut(s) 441
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 2 cut(s) 322, 353
ApeKI GCWGC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 322
BanII GRGCYC 1 cut(s) 441
BauI CACGAG 1 cut(s) 218
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 183
BccI CCATC 2 cut(s) 223, 259
BciT130I CCWGG 1 cut(s) 352
BclI TGATCA 1 cut(s) 139
BfaI CTAG 2 cut(s) 108, 463
BfmI CTRYAG 1 cut(s) 166
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 352
BmrI ACTGGG 2 cut(s) 280, 331
BmsI GCATC 1 cut(s) 128
BmuI ACTGGG 2 cut(s) 280, 331
Bsa29I ATCGAT 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 90, 204
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 319, 433
Bse1I ACTGG 2 cut(s) 275, 337
BseAI TCCGGA 1 cut(s) 204
BseBI CCWGG 1 cut(s) 352
BseCI ATCGAT 1 cut(s) 280
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 3 cut(s) 68, 319, 433
BseNI ACTGG 2 cut(s) 275, 337
BseXI GCAGC 1 cut(s) 183
BshFI GGCC 2 cut(s) 324, 355
BshVI ATCGAT 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 3 cut(s) 69, 91, 205
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 3 cut(s) 68, 319, 433
BsmFI GGGAC 1 cut(s) 319
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 2 cut(s) 324, 355
Bsp1286I GDGCHC 1 cut(s) 441
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 3 cut(s) 139, 384, 454
BspANI GGCC 2 cut(s) 324, 355
BspDI ATCGAT 1 cut(s) 280
BspEI TCCGGA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 275, 337
BssMI GATC 3 cut(s) 139, 384, 454
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 2 cut(s) 31, 203
BstC8I GCNNGC 3 cut(s) 176, 398, 441
BstF5I GGATG 1 cut(s) 270
BstKTI GATC 3 cut(s) 142, 387, 457
BstMBI GATC 3 cut(s) 139, 384, 454
BstMWI GCNNNNNNNGC 2 cut(s) 143, 377
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 183
Bsu15I ATCGAT 1 cut(s) 280
BsuRI GGCC 2 cut(s) 324, 355
BsuTUI ATCGAT 1 cut(s) 280
BtsCI GGATG 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 3 cut(s) 176, 398, 441
Cfr13I GGNCC 1 cut(s) 322
ClaI ATCGAT 1 cut(s) 280
Csp6I GTAC 1 cut(s) 154
CviAII CATG 3 cut(s) 143, 193, 319
CviJI RGCY 7 cut(s) 137, 178, 250, 324, 355, 380, 439
CviKI_1 RGCY 7 cut(s) 137, 178, 250, 324, 355, 380, 439
CviQI GTAC 1 cut(s) 154
DpnI GATC 3 cut(s) 141, 386, 456
DpnII GATC 3 cut(s) 139, 384, 454
Eam1104I CTCTTC 2 cut(s) 31, 203
EarI CTCTTC 2 cut(s) 31, 203
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 441
FaeI CATG 3 cut(s) 146, 196, 322
FaiI YATR 5 cut(s) 144, 194, 308, 310, 320
FaqI GGGAC 1 cut(s) 319
FatI CATG 3 cut(s) 142, 192, 318
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 172
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 1 cut(s) 172
FspBI CTAG 2 cut(s) 108, 463
GluI GCNGC 1 cut(s) 172
HaeIII GGCC 2 cut(s) 324, 355
HapII CCGG 3 cut(s) 69, 91, 205
Hin1II CATG 3 cut(s) 146, 196, 322
HinfI GANTC 2 cut(s) 277, 299
HpaII CCGG 3 cut(s) 69, 91, 205
Hpy166II GTNNAC 2 cut(s) 184, 217
Hpy188I TCNGA 1 cut(s) 259
Hpy188III TCNNGA 2 cut(s) 205, 218
Hpy8I GTNNAC 2 cut(s) 184, 217
Hpy99I CGWCG 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 2 cut(s) 45, 96
HpyCH4V TGCA 3 cut(s) 119, 146, 331
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 377
HpySE526I ACGT 2 cut(s) 45, 96
Hsp92II CATG 3 cut(s) 146, 196, 322
Kpn2I TCCGGA 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 3 cut(s) 139, 384, 454
LmnI GCTCC 2 cut(s) 134, 436
LpnPI CCDG 9 cut(s) 82, 104, 160, 164, 218, 256, 337, 350, 364
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 128
MaeI CTAG 2 cut(s) 108, 463
MaeII ACGT 2 cut(s) 45, 96
MalI GATC 3 cut(s) 141, 386, 456
MboI GATC 3 cut(s) 139, 384, 454
MboII GAAGA 8 cut(s) 18, 21, 24, 93, 220, 238, 415, 449
MhlI GDGCHC 1 cut(s) 441
MmeI TCCRAC 2 cut(s) 66, 237
MnlI CCTC 4 cut(s) 34, 231, 261, 335
MroI TCCGGA 1 cut(s) 204
MspI CCGG 3 cut(s) 69, 91, 205
MspR9I CCNGG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 143, 377
NdeII GATC 3 cut(s) 139, 384, 454
NlaIII CATG 3 cut(s) 146, 196, 322
NmeAIII GCCGAG 1 cut(s) 381
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 393
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 277, 299
PflMI CCANNNNNTGG 2 cut(s) 319, 433
PkrI GCNGC 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 441
Psp1406I AACGTT 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspPI GGNCC 1 cut(s) 322
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SacI GAGCTC 1 cut(s) 441
SatI GCNGC 1 cut(s) 172
Sau3AI GATC 3 cut(s) 139, 384, 454
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 352
SduI GDGCHC 1 cut(s) 441
SetI ASST 5 cut(s) 48, 99, 139, 296, 441
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 1 cut(s) 166
SspMI CTAG 2 cut(s) 108, 463
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 2 cut(s) 48, 99
TaqI TCGA 2 cut(s) 122, 280
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 2 cut(s) 277, 299
TscAI CASTG 1 cut(s) 21
TseI GCWGC 1 cut(s) 171
TspDTI ATGAA 2 cut(s) 239, 359
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 2 cut(s) 319, 433
XspI CTAG 2 cut(s) 108, 463
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.