Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002735

Physical Location & Seq

Genomic Coordinates Forward (+)

6703028 .. 6706701

Gene Structure 3674 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1095200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGCACGCAAGAAGATCAGAGAGTACGACTCCAAGAGATTGTTGAAGGAGCACTTCAAGAGGCTTGCCGGCCGCGATTTGCCTCTTAAATCTGCCCAAATTACCGAAACAACCGATGTCAATGAGCTGCTGGAGAAGGAATCGTGGCTTTCGTCTTCCAAACTCGTTGTGAAGCCTGACATGTTGTTCGGAAAGCGTGGGAAGAGCGGTTTGGTTGCCCTGAATCTTGATTTTGCTCAAGTTGTCGAATTTGTGAAGGAGCGCCTAGGCAAAGAGGTCGAGATGGGTGGCTGCAAGGGACCAATAACCACGTTCATTGTCGAACCCTTCATTCCCCACAACGAAGAGTTTTACCTTAATATTGTCTCAGACCGACTTGGAAACAGCATAAGCTTCTCCGAATGTGGAGGAATTGACATTGAGGAGAACTGGGATAAGGTAAAGACCATATTTGTCCCTACTGGAGTTGCTTTTTCATCAGAAGTGTCTGCTCCACTTGTTGCAACCCTACCCTTGGAGATCAAAGCAGAACTCGAGGACTTTATCAAAGCCGTATTTGCTCTATTTCAAGATTTGGACTTCACATTTCTGGAGATGAATCCTTTTGCATTGGTTGATGGAAAGCCATATCCTTTGGATATGAGAGGCGAGCTGGATGACACTGCTGCTTTCAAGAATTTCAAAAAGTGGGGCAGTATTGAATTCCCAATGCCTTTTGGAAGAGTTATGAGTCCTACAGAGAAATTTATTCACGGGCTAGATGAAAAGACAAGTGCATCTTTGAAATTCACAGTTCTGAACCCAAAGGGGCGAATTTGGACTATGGTCGCAGGAGGAGGTGCAAGTGTCATCTACGCAGACACGGTTGGAGATCTTGGCTTTGCTGATGAGCTTGGAAACTATGCTGAATACAGTGGAGCACCCAATGAAGAGGAGGTGTTGCAGTACGCCAGAGTTGTGATTGATTGTGCAACTTCTGAGCCTGACGGTCGTAAGCGAGCCCTTGTAATAGGAGGAGGTATTGCTAACTTTACCGATGTTGCTGCAACATTCAATGGCATTATTCGTGCCTTGAAGGAGAAGGAATCTAAGCTTAAAGCCGCACAGATGCATCTTTACGTCAGGAGAGGAGGCCCCAACTACCAGAGGGGGCTTGCAAAAATGCGGGCACTTGCAGAGGAAGTCGGCCTCCCTATTGAGGTCTATGGACCCGAAGCAACAATGACCGGTATTTGCAAACAGGCAATTCAGTGCATCACCGCAGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003878 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006633 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009346 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0032991 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046394 GO:0046483 GO:0046912 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00020 ko00720 ko01100 ko01110 ko01120 ko01130 map00020 map00720 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

ATP-grasp_2 Citrate_bind

Protein Analysis

424

Amino Acids

46.66

Weight (kDa)

5.48

Isoelectric Point (pI)

34.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synth_N	PF24948	2 - 230	1.9e-74	ATP-citrate synthase ATP-grasp domain
ATP-grasp_2	PF08442	6 - 203	3.8e-17	ATP-grasp domain
Citrate_bind	PF16114	241 - 417	4.8e-84	ATP citrate lyase citrate-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10670 AT1G10670 AT1G10670 AT1G10670 AT1G60810 AT1G60810
fragaria_vesca	FvH4_4g32950 FvH4_4g32950 FvH4_4g32950
malus_domestica	MD13G1095200.v1.1 MD16G1096300.v1.1
prunus_persica	Prupe.1G250100_v2.0.a1 Prupe.1G250100_v2.0.a1
pyrus_communis	pycom05g10590 pycom13g08300 pycom16g08220
rosa_chinensis	RchiOBHm_Chr4g0441981 RchiOBHm_Chr7g0181761
rosa_laevigata	RLG00000006053 RLG00000018726
rosa_multiflora	Rmu_co8081680.1_g000001 Rmu_sc0000905.1_g000020
rosa_roxburghii	Rroxscaffold_1G00051200 Rroxscaffold_5G00359300 Rroxscaffold_5G00382850
rosa_rugosa	Rorug04G0337500 Rorug04G0337500 Rorug04G0337500
rosa_samantha	Rh2BG270200 Rh4AG389500 Rh4BG402900 Rh4CG418100 Rh4DG118600 Rh4DG396000 Rh7AG279300 Rh7CG298400 Rh7DG045400
rosa_wichuraiana	Rw4G033630 Rw6G021030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	207
AccII	CGCG	1 cut(s)	75
AciI	CCGC	5 cut(s)	73, 207, 1101, 1165, 1260
AcoI	YGGCCR	1 cut(s)	70
AcsI	RAATTY	6 cut(s)	248, 676, 701, 743, 785, 813
AfaI	GTAC	2 cut(s)	26, 947
AfiI	CCNNNNNNNGG	2 cut(s)	514, 1198
AflIII	ACRYGT	1 cut(s)	180
AgeI	ACCGGT	1 cut(s)	1226
AgsI	TTSAA	9 cut(s)	46, 58, 569, 673, 682, 701, 784, 1054, 1075
AjuI	GAANNNNNNNTTGG	2 cut(s)	194, 226
AluBI	AGCT	6 cut(s)	127, 393, 652, 892, 1093, 1265
AluI	AGCT	6 cut(s)	127, 393, 652, 892, 1093, 1265
Alw21I	GWGCWC	2 cut(s)	54, 922
Alw26I	GTCTC	1 cut(s)	370
Ama87I	CYCGRG	1 cut(s)	533
AoxI	GGCC	3 cut(s)	70, 1132, 1186
ApeKI	GCWGC	6 cut(s)	127, 291, 665, 1043, 1262, 1265
ApoI	RAATTY	6 cut(s)	248, 676, 701, 743, 785, 813
ArsI	GACNNNNNNTTYG	2 cut(s)	170, 202
AsiGI	ACCGGT	1 cut(s)	1226
AspA2I	CCTAGG	1 cut(s)	265
AspLEI	GCGC	1 cut(s)	264
AspS9I	GGNCC	3 cut(s)	299, 1133, 1208
AsuHPI	GGTGA	1 cut(s)	1249
AvaI	CYCGRG	1 cut(s)	533
AvaII	GGWCC	2 cut(s)	299, 1208
AvrII	CCTAGG	1 cut(s)	265
BaeGI	GKGCMC	1 cut(s)	1171
BanII	GRGCYC	1 cut(s)	1003
BbsI	GAAGAC	1 cut(s)	147
Bbv12I	GWGCWC	2 cut(s)	54, 922
BbvI	GCAGC	5 cut(s)	114, 278, 652, 1030, 1252
BccI	CCATC	2 cut(s)	277, 611
BceAI	ACGGC	1 cut(s)	536
BcgI	CGANNNNNNTGC	4 cut(s)	259, 293, 1025, 1059
BcoDI	GTCTC	1 cut(s)	370
BfaI	CTAG	2 cut(s)	266, 758
BfmI	CTRYAG	1 cut(s)	735
BfoI	RGCGCY	1 cut(s)	265
BglII	AGATCT	1 cut(s)	871
BisI	GCNGC	8 cut(s)	73, 128, 292, 666, 1044, 1101, 1263, 1266
BlnI	CCTAGG	1 cut(s)	265
BlsI	GCNGC	8 cut(s)	74, 129, 293, 667, 1045, 1102, 1264, 1267
Bme18I	GGWCC	2 cut(s)	299, 1208
BmeT110I	CYCGRG	1 cut(s)	533
BmgT120I	GGNCC	3 cut(s)	299, 1133, 1208
BmiI	GGNNCC	3 cut(s)	300, 1135, 1210
BmrI	ACTGGG	1 cut(s)	439
BmsI	GCATC	4 cut(s)	785, 1098, 1120, 1263
BmuI	ACTGGG	1 cut(s)	439
BoxI	GACNNNNGTC	1 cut(s)	824
BpiI	GAAGAC	1 cut(s)	147
BplI	GAGNNNNNCTC	2 cut(s)	14, 46
BpmI	CTGGAG	3 cut(s)	152, 483, 611
BpuEI	CTTGAG	1 cut(s)	222
BsaJI	CCNNGG	2 cut(s)	265, 513
BsaWI	WCCGGW	1 cut(s)	1226
Bsc4I	CCNNNNNNNGG	2 cut(s)	514, 1198
Bse118I	RCCGGY	2 cut(s)	68, 1226
Bse1I	ACTGG	2 cut(s)	434, 466
BseDI	CCNNGG	2 cut(s)	265, 513
BseGI	GGATG	1 cut(s)	661
BseLI	CCNNNNNNNGG	2 cut(s)	514, 1198
BseMII	CTCAG	2 cut(s)	381, 969
BseNI	ACTGG	2 cut(s)	434, 466
BseRI	GAGGAG	5 cut(s)	437, 849, 947, 1029, 1143
BseSI	GKGCMC	1 cut(s)	1171
BseX3I	CGGCCG	1 cut(s)	70
BseXI	GCAGC	5 cut(s)	114, 278, 652, 1030, 1252
Bsh1236I	CGCG	1 cut(s)	75
Bsh1285I	CGRYCG	2 cut(s)	73, 991
BshFI	GGCC	3 cut(s)	72, 1134, 1188
BshTI	ACCGGT	1 cut(s)	1226
BsiEI	CGRYCG	2 cut(s)	73, 991
BsiHKAI	GWGCWC	2 cut(s)	54, 922
BsiHKCI	CYCGRG	1 cut(s)	533
BsiSI	CCGG	2 cut(s)	69, 1227
BslFI	GGGAC	2 cut(s)	312, 440
BslI	CCNNNNNNNGG	2 cut(s)	514, 1198
BsmAI	GTCTC	1 cut(s)	370
BsmFI	GGGAC	2 cut(s)	312, 440
BsnI	GGCC	3 cut(s)	72, 1134, 1188
BsoBI	CYCGRG	1 cut(s)	533
Bsp1286I	GDGCHC	4 cut(s)	54, 922, 1003, 1171
Bsp143I	GATC	3 cut(s)	15, 519, 871
BspACI	CCGC	5 cut(s)	73, 207, 1101, 1165, 1260
BspANI	GGCC	3 cut(s)	72, 1134, 1188
BspCNI	CTCAG	2 cut(s)	380, 970
BspFNI	CGCG	1 cut(s)	75
BspLI	GGNNCC	3 cut(s)	300, 1135, 1210
BspQI	GCTCTTC	1 cut(s)	197
BsrBI	CCGCTC	1 cut(s)	207
BsrFI	RCCGGY	2 cut(s)	68, 1226
BsrI	ACTGG	2 cut(s)	434, 466
BssAI	RCCGGY	2 cut(s)	68, 1226
BssECI	CCNNGG	2 cut(s)	265, 513
BssMI	GATC	3 cut(s)	15, 519, 871
BssT1I	CCWWGG	2 cut(s)	265, 513
Bst4CI	ACNGT	4 cut(s)	793, 865, 914, 989
Bst6I	CTCTTC	4 cut(s)	197, 339, 715, 924
BstC8I	GCNNGC	7 cut(s)	7, 66, 70, 650, 999, 1155, 1167
BstDEI	CTNAG	3 cut(s)	367, 978, 1089
BstF5I	GGATG	1 cut(s)	661
BstFNI	CGCG	1 cut(s)	75
BstH2I	RGCGCY	1 cut(s)	265
BstHHI	GCGC	1 cut(s)	264
BstKTI	GATC	3 cut(s)	18, 522, 874
BstMAI	GTCTC	1 cut(s)	370
BstMBI	GATC	3 cut(s)	15, 519, 871
BstMCI	CGRYCG	2 cut(s)	73, 991
BstMWI	GCNNNNNNNGC	1 cut(s)	557
BstNSI	RCATGY	1 cut(s)	184
BstPAI	GACNNNNGTC	1 cut(s)	824
BstSFI	CTRYAG	1 cut(s)	735
BstSLI	GKGCMC	1 cut(s)	1171
BstUI	CGCG	1 cut(s)	75
BstV1I	GCAGC	5 cut(s)	114, 278, 652, 1030, 1252
BstV2I	GAAGAC	1 cut(s)	147
BstX2I	RGATCY	1 cut(s)	871
BstYI	RGATCY	1 cut(s)	871
BstZI	CGGCCG	1 cut(s)	70
BsuRI	GGCC	3 cut(s)	72, 1134, 1188
BtsCI	GGATG	1 cut(s)	661
BtsI	GCAGTG	1 cut(s)	660
BtsIMutI	CAGTG	3 cut(s)	660, 919, 1256
Cac8I	GCNNGC	7 cut(s)	7, 66, 70, 650, 999, 1155, 1167
CfoI	GCGC	1 cut(s)	264
Cfr10I	RCCGGY	2 cut(s)	68, 1226
Cfr13I	GGNCC	3 cut(s)	299, 1133, 1208
Csp6I	GTAC	2 cut(s)	25, 946
CspAI	ACCGGT	1 cut(s)	1226
CspCI	CAANNNNNGTGG	2 cut(s)	298, 333
CviAII	CATG	1 cut(s)	181
CviQI	GTAC	2 cut(s)	25, 946
DdeI	CTNAG	3 cut(s)	367, 978, 1089
DpnI	GATC	3 cut(s)	17, 521, 873
DpnII	GATC	3 cut(s)	15, 519, 871
EaeI	YGGCCR	1 cut(s)	70
EagI	CGGCCG	1 cut(s)	70
Eam1104I	CTCTTC	4 cut(s)	197, 339, 715, 924
EarI	CTCTTC	4 cut(s)	197, 339, 715, 924
EclXI	CGGCCG	1 cut(s)	70
Eco130I	CCWWGG	2 cut(s)	265, 513
Eco24I	GRGCYC	1 cut(s)	1003
Eco47I	GGWCC	2 cut(s)	299, 1208
Eco52I	CGGCCG	1 cut(s)	70
Eco88I	CYCGRG	1 cut(s)	533
EcoO109I	RGGNCCY	1 cut(s)	1133
EcoRI	GAATTC	1 cut(s)	701
EcoT14I	CCWWGG	2 cut(s)	265, 513
EcoT22I	ATGCAT	1 cut(s)	1113
EcoT38I	GRGCYC	1 cut(s)	1003
ErhI	CCWWGG	2 cut(s)	265, 513
FaeI	CATG	1 cut(s)	184
FalI	AAGNNNNNCTT	4 cut(s)	38, 70, 763, 795
FaqI	GGGAC	2 cut(s)	312, 440
FatI	CATG	1 cut(s)	180
FauI	CCCGC	1 cut(s)	1158
Fnu4HI	GCNGC	8 cut(s)	73, 128, 292, 666, 1044, 1101, 1263, 1266
FokI	GGATG	1 cut(s)	668
FriOI	GRGCYC	1 cut(s)	1003
Fsp4HI	GCNGC	8 cut(s)	73, 128, 292, 666, 1044, 1101, 1263, 1266
FspBI	CTAG	2 cut(s)	266, 758
GlaI	GCGC	1 cut(s)	263
GluI	GCNGC	8 cut(s)	73, 128, 292, 666, 1044, 1101, 1263, 1266
GsuI	CTGGAG	3 cut(s)	152, 483, 611
HaeII	RGCGCY	1 cut(s)	265
HaeIII	GGCC	3 cut(s)	72, 1134, 1188
HapII	CCGG	2 cut(s)	69, 1227
HhaI	GCGC	1 cut(s)	264
Hin1II	CATG	1 cut(s)	184
Hin6I	GCGC	1 cut(s)	262
HinP1I	GCGC	1 cut(s)	262
HindIII	AAGCTT	2 cut(s)	391, 1091
HinfI	GANTC	6 cut(s)	29, 140, 223, 598, 730, 1085
HpaII	CCGG	2 cut(s)	69, 1227
HphI	GGTGA	1 cut(s)	1249
Hpy188I	TCNGA	7 cut(s)	20, 191, 370, 400, 481, 798, 979
Hpy188III	TCNNGA	7 cut(s)	58, 227, 280, 569, 590, 673, 1123
HpyAV	CCTTC	6 cut(s)	40, 130, 250, 337, 1069, 1075
HpyCH4III	ACNGT	4 cut(s)	793, 865, 914, 989
HpyCH4IV	ACGT	2 cut(s)	311, 1119
HpyF10VI	GCNNNNNNNGC	1 cut(s)	557
HpyF3I	CTNAG	3 cut(s)	367, 978, 1089
HpySE526I	ACGT	2 cut(s)	311, 1119
Hsp92II	CATG	1 cut(s)	184
HspAI	GCGC	1 cut(s)	262
KroI	GCCGGC	1 cut(s)	68
KroNI	GCCGGC	1 cut(s)	70
Kzo9I	GATC	3 cut(s)	15, 519, 871
LguI	GCTCTTC	1 cut(s)	197
LmnI	GCTCC	4 cut(s)	49, 259, 496, 917
Lsp1109I	GCAGC	5 cut(s)	114, 278, 652, 1030, 1252
LweI	GCATC	4 cut(s)	785, 1098, 1120, 1263
MaeI	CTAG	2 cut(s)	266, 758
MaeII	ACGT	2 cut(s)	311, 1119
MalI	GATC	3 cut(s)	17, 521, 873
MbiI	CCGCTC	1 cut(s)	207
MboI	GATC	3 cut(s)	15, 519, 871
MboII	GAAGA	6 cut(s)	25, 147, 214, 356, 732, 941
MflI	RGATCY	1 cut(s)	871
MhlI	GDGCHC	4 cut(s)	54, 922, 1003, 1171
MluCI	AATT	9 cut(s)	99, 248, 411, 676, 701, 743, 785, 813, 1245
MlyI	GAGTC	2 cut(s)	23, 739
MmeI	TCCRAC	1 cut(s)	847
Mph1103I	ATGCAT	1 cut(s)	1113
MroNI	GCCGGC	1 cut(s)	68
MseI	TTAA	3 cut(s)	87, 357, 1095
MspA1I	CMGCKG	1 cut(s)	1265
MspI	CCGG	2 cut(s)	69, 1227
MvnI	CGCG	1 cut(s)	75
MwoI	GCNNNNNNNGC	1 cut(s)	557
NaeI	GCCGGC	1 cut(s)	70
NdeII	GATC	3 cut(s)	15, 519, 871
NgoMIV	GCCGGC	1 cut(s)	68
NlaIII	CATG	1 cut(s)	184
NlaIV	GGNNCC	3 cut(s)	300, 1135, 1210
NsiI	ATGCAT	1 cut(s)	1113
NspI	RCATGY	1 cut(s)	184
PaeR7I	CTCGAG	1 cut(s)	533
PciI	ACATGT	1 cut(s)	180
PciSI	GCTCTTC	1 cut(s)	197
PcsI	WCGNNNNNNNCGW	1 cut(s)	149
PdiI	GCCGGC	1 cut(s)	70
PfeI	GAWTC	4 cut(s)	140, 223, 598, 1085
PinAI	ACCGGT	1 cut(s)	1226
PkrI	GCNGC	8 cut(s)	74, 129, 293, 667, 1045, 1102, 1264, 1267
PleI	GAGTC	2 cut(s)	23, 738
PpsI	GAGTC	2 cut(s)	23, 738
PscI	ACATGT	1 cut(s)	180
PshAI	GACNNNNGTC	1 cut(s)	824
PspN4I	GGNNCC	3 cut(s)	300, 1135, 1210
PspPI	GGNCC	3 cut(s)	299, 1133, 1208
PspXI	VCTCGAGB	1 cut(s)	533
PsuI	RGATCY	1 cut(s)	871
PvuII	CAGCTG	1 cut(s)	1265
RsaI	GTAC	2 cut(s)	26, 947
RsaNI	GTAC	2 cut(s)	25, 946
SapI	GCTCTTC	1 cut(s)	197
SaqAI	TTAA	3 cut(s)	87, 357, 1095
SatI	GCNGC	8 cut(s)	73, 128, 292, 666, 1044, 1101, 1263, 1266
Sau3AI	GATC	3 cut(s)	15, 519, 871
Sau96I	GGNCC	3 cut(s)	299, 1133, 1208
SchI	GAGTC	2 cut(s)	23, 739
SduI	GDGCHC	4 cut(s)	54, 922, 1003, 1171
SfaNI	GCATC	4 cut(s)	785, 1098, 1120, 1263
SfcI	CTRYAG	1 cut(s)	735
Sfr274I	CTCGAG	1 cut(s)	533
SinI	GGWCC	2 cut(s)	299, 1208
SlaI	CTCGAG	1 cut(s)	533
SmlI	CTYRAG	2 cut(s)	237, 533
SmoI	CTYRAG	2 cut(s)	237, 533
Sse9I	AATT	9 cut(s)	99, 248, 411, 676, 701, 743, 785, 813, 1245
SsiI	CCGC	5 cut(s)	73, 207, 1101, 1165, 1260
SspI	AATATT	1 cut(s)	361
SspMI	CTAG	2 cut(s)	266, 758
StyI	CCWWGG	2 cut(s)	265, 513
TaaI	ACNGT	4 cut(s)	793, 865, 914, 989
TaiI	ACGT	2 cut(s)	314, 1122
TaqI	TCGA	4 cut(s)	246, 279, 321, 534
TaqII	GACCGA	1 cut(s)	387
TasI	AATT	9 cut(s)	99, 248, 411, 676, 701, 743, 785, 813, 1245
TauI	GCSGC	2 cut(s)	75, 1103
TfiI	GAWTC	4 cut(s)	140, 223, 598, 1085
Tru1I	TTAA	3 cut(s)	87, 357, 1095
Tru9I	TTAA	3 cut(s)	87, 357, 1095
TscAI	CASTG	3 cut(s)	667, 919, 1256
TseI	GCWGC	6 cut(s)	127, 291, 665, 1043, 1262, 1265
TspDTI	ATGAA	6 cut(s)	304, 319, 465, 611, 777, 942
TspRI	CASTG	3 cut(s)	667, 919, 1256
VpaK11BI	GGWCC	2 cut(s)	299, 1208
XapI	RAATTY	6 cut(s)	248, 676, 701, 743, 785, 813
XceI	RCATGY	1 cut(s)	184
XhoI	CTCGAG	1 cut(s)	533
XmaJI	CCTAGG	1 cut(s)	265
XspI	CTAG	2 cut(s)	266, 758
Zsp2I	ATGCAT	1 cut(s)	1113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.