MD13G1109400.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008131
Physical Location & Seq
Forward (+)
7929130 .. 7930494
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1109400.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGAAGATTGAAGTTGAAGTGATCTCCAAGTCCATAGAGATGATCAAACCATTTTCTCCAACCCCAAATCATCTTCACTATCACCAGCTCTCCTTTCTCGATCAATTAGCTCCCCACGCATATATCCCCTTTCTCTTCTTCTACGAATCCACCGGCACCAAAACCATCACTCAGATATCAAACATCCTCAAGACGTCTCTCTCCAAAATATTATCCCAATACTACCCTTTTGCCGGCCGAGTGAAACACAACCTCTTTGTTCACTGCAGCGACCAAGGCGTCCCATTCTTCGAATCCAAAGTCAAATCCCAACCCCTCTCTGATATGATCGCCAGCCCCTTTGTCTCCGAACTCAACAAGTTGCTGCCCTACAGGCTAGGCGAAGTTACTGAAGTACCCCTTGGTGTTCAACTTAATGTGTTTGACTGTGGAGGATTTGCTATTGGCTTGTGTGTTTCTCATAGGATTGCAGATGGGTTAACATCTTTTACATTTGTGAACAGATGGGCAGCTCAAGCTAATTGTGATGCACAAGGGAAGAGTAATATTATTGTGGGTCCGGATTTTTCTGCAGCATCAATCTTCCCACCAAAAAATATGGATGGGTATTTAGGGGTTTCGATTGTCAACAAGAAAGCAATCGTAACAAAGAGGTTTGTGTTCGATGGCTCAAAGGTTCAGGCTTTAAGATCGAGATATGAAGAGAGCATGAACTTTTCCAAAACCCAAAAACGCCCTTCAAAAGTTGAGGCCTTATCAGCTTTATTATGGAACATCTTTCTGGCATCAAGGAGAGAAAGAGATTGTGTCGCTATTTCAAATCAATCGCGCACCCAAACATTATACACGGTTGTTTATATTATGAATCTGCGGTCGAGGTTTGACCCACCTTTGCCTCAGCATGCTTTTGGGAATTATTACAGGGCTGCCACTGCAACTCCAACTTTGGTCAATGGGGAGGAGAGCCATCGATTGGTGAGGCAGGTGATAGAGGAAATTGAGAACATTGACAATAATTATATGAGGAGATTTCAAGAGGGATATCGAGAGCATTTGGATTTCATGAGGAAAAGAATGGAGAGGTTTGCAAAAGGTGAGCTGGTGACATTGACTTTTTCGAGTGTGTGCAGGTTTCCTATTTACGATGCTGATTTTGGTTGGGGGAAGCCTGCATGGGTGAGCATGGCTGCCATGGATATCAATAATCAGATAGTTTTCATGGACACCAAAAATGGCGATGGTATCGAGTCGTATTTTAGCTTGACAGAGGAAGACATGGCCAAGTTTGAACTTCACAAAGAGTTCATCGCATTGCTTAAATCTCCAGTTGGTAATGTAAAGGAAAATTCACTTTCACGTCTTTAA

Protein Analysis

455

Amino Acids

51.63

Weight (kDa)

8.82

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 435 8.3e-77 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 973
AasI GACNNNNNNGTC 1 cut(s) 278
AatII GACGTC 1 cut(s) 197
Acc36I ACCTGC 2 cut(s) 973, 1119
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 973
AccII CGCG 1 cut(s) 829
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 1 cut(s) 871
AcoI YGGCCR 2 cut(s) 235, 1278
AcsI RAATTY 1 cut(s) 1345
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 2 cut(s) 194, 279
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 233, 973
AgsI TTSAA 7 cut(s) 11, 17, 410, 741, 819, 1034, 1289
AjiI CACGTC 1 cut(s) 1358
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 7 cut(s) 88, 110, 512, 518, 761, 1099, 1260
AluI AGCT 7 cut(s) 88, 110, 512, 518, 761, 1099, 1260
Alw26I GTCTC 2 cut(s) 201, 349
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 3 cut(s) 235, 750, 1278
ApeKI GCWGC 6 cut(s) 267, 364, 509, 572, 926, 1187
ApoI RAATTY 1 cut(s) 1345
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
AspLEI GCGC 1 cut(s) 831
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 6 cut(s) 74, 988, 997, 1106, 1114, 1189
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 557
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BalI TGGCCA 1 cut(s) 1280
BanI GGYRCC 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 1278
BbvCI CCTCAGC 1 cut(s) 897
BbvI GCAGC 6 cut(s) 279, 351, 521, 584, 913, 1174
BccI CCATC 7 cut(s) 173, 467, 498, 596, 659, 975, 1232
BclI TGATCA 1 cut(s) 42
BcoDI GTCTC 2 cut(s) 201, 349
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 3 cut(s) 265, 370, 570
BfuAI ACCTGC 2 cut(s) 973, 1119
BglI GCCNNNNNGGC 1 cut(s) 373
BisI GCNGC 6 cut(s) 268, 365, 510, 573, 927, 1188
BlsI GCNGC 6 cut(s) 269, 366, 511, 574, 928, 1189
Bme18I GGWCC 1 cut(s) 557
BmgBI CACGTC 1 cut(s) 1358
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 2 cut(s) 157, 558
BmsI GCATC 4 cut(s) 517, 584, 794, 1135
BpiI GAAGAC 1 cut(s) 1278
BpmI CTGGAG 1 cut(s) 1308
Bpu10I CCTNAGC 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 291
BpuEI CTTGAG 2 cut(s) 173, 498
Bsa29I ATCGAT 1 cut(s) 970
BsaHI GRCGYC 2 cut(s) 194, 279
BsaJI CCNNGG 3 cut(s) 274, 400, 1191
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 973
Bse118I RCCGGY 2 cut(s) 152, 233
Bse1I ACTGG 1 cut(s) 1325
Bse3DI GCAATG 1 cut(s) 1310
BseAI TCCGGA 1 cut(s) 559
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 3 cut(s) 274, 400, 1191
BseGI GGATG 2 cut(s) 183, 607
BseLI CCNNNNNNNGG 2 cut(s) 233, 973
BseMI GCAATG 1 cut(s) 1310
BseMII CTCAG 2 cut(s) 185, 911
BseNI ACTGG 1 cut(s) 1325
BseRI GAGGAG 2 cut(s) 974, 1039
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 6 cut(s) 279, 351, 521, 584, 913, 1174
BsgI GTGCAG 1 cut(s) 1147
Bsh1236I CGCG 1 cut(s) 829
Bsh1285I CGRYCG 2 cut(s) 238, 875
BshFI GGCC 3 cut(s) 237, 752, 1280
BshNI GGYRCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 970
BsiEI CGRYCG 2 cut(s) 238, 875
BsiSI CCGG 3 cut(s) 153, 234, 560
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 233, 973
BsmAI GTCTC 2 cut(s) 201, 349
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 3 cut(s) 237, 752, 1280
Bsp119I TTCGAA 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 559
Bsp143I GATC 5 cut(s) 21, 42, 100, 327, 689
Bsp19I CCATGG 1 cut(s) 1191
BspACI CCGC 1 cut(s) 871
BspANI GGCC 3 cut(s) 237, 752, 1280
BspCNI CTCAG 2 cut(s) 184, 910
BspDI ATCGAT 1 cut(s) 970
BspEI TCCGGA 1 cut(s) 559
BspFNI CGCG 1 cut(s) 829
BspHI TCATGA 1 cut(s) 1062
BspLI GGNNCC 2 cut(s) 157, 558
BspMAI CTGCAG 2 cut(s) 269, 574
BspMI ACCTGC 2 cut(s) 973, 1119
BspT104I TTCGAA 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 1310
BsrFI RCCGGY 2 cut(s) 152, 233
BsrI ACTGG 1 cut(s) 1325
BssAI RCCGGY 2 cut(s) 152, 233
BssECI CCNNGG 3 cut(s) 274, 400, 1191
BssMI GATC 5 cut(s) 21, 42, 100, 327, 689
BssNI GRCGYC 2 cut(s) 194, 279
BssT1I CCWWGG 3 cut(s) 274, 400, 1191
Bst4CI ACNGT 2 cut(s) 428, 850
Bst6I CTCTTC 3 cut(s) 140, 533, 696
BstACI GRCGYC 2 cut(s) 194, 279
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 235, 334, 903, 1170
BstDEI CTNAG 2 cut(s) 171, 897
BstDSI CCRYGG 1 cut(s) 1191
BstF5I GGATG 2 cut(s) 183, 607
BstFNI CGCG 1 cut(s) 829
BstHHI GCGC 1 cut(s) 831
BstKTI GATC 5 cut(s) 24, 45, 103, 330, 692
BstMAI GTCTC 2 cut(s) 201, 349
BstMBI GATC 5 cut(s) 21, 42, 100, 327, 689
BstMCI CGRYCG 2 cut(s) 238, 875
BstMWI GCNNNNNNNGC 6 cut(s) 116, 276, 373, 515, 758, 932
BstNSI RCATGY 1 cut(s) 905
BstSFI CTRYAG 3 cut(s) 265, 370, 570
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 6 cut(s) 279, 351, 521, 584, 913, 1174
BstV2I GAAGAC 1 cut(s) 1278
BstZI CGGCCG 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 970
BsuRI GGCC 3 cut(s) 237, 752, 1280
BsuTUI ATCGAT 1 cut(s) 970
BtgI CCRYGG 1 cut(s) 1191
BtgZI GCGATG 2 cut(s) 1251, 1291
BtrI CACGTC 1 cut(s) 1358
BtsCI GGATG 2 cut(s) 183, 607
BtsI GCAGTG 2 cut(s) 262, 930
BtsIMutI CAGTG 2 cut(s) 262, 930
BveI ACCTGC 2 cut(s) 973, 1119
Cac8I GCNNGC 4 cut(s) 235, 334, 903, 1170
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 1 cut(s) 831
Cfr10I RCCGGY 2 cut(s) 152, 233
Cfr13I GGNCC 1 cut(s) 557
ClaI ATCGAT 1 cut(s) 970
CseI GACGC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 395
CviAII CATG 8 cut(s) 709, 902, 1063, 1173, 1183, 1192, 1219, 1276
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 2 cut(s) 171, 897
DpnI GATC 5 cut(s) 23, 44, 102, 329, 691
DpnII GATC 5 cut(s) 21, 42, 100, 327, 689
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 2 cut(s) 235, 1278
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 140, 533, 696
EarI CTCTTC 3 cut(s) 140, 533, 696
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 274, 400, 1191
Eco147I AGGCCT 1 cut(s) 752
Eco32I GATATC 3 cut(s) 177, 1043, 1198
Eco47I GGWCC 1 cut(s) 557
Eco52I CGGCCG 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 411
EcoRV GATATC 3 cut(s) 177, 1043, 1198
EcoT14I CCWWGG 3 cut(s) 274, 400, 1191
ErhI CCWWGG 3 cut(s) 274, 400, 1191
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 8 cut(s) 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 266
FatI CATG 8 cut(s) 708, 901, 1062, 1172, 1182, 1191, 1218, 1275
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 6 cut(s) 268, 365, 510, 573, 927, 1188
FokI GGATG 2 cut(s) 170, 614
Fsp4HI GCNGC 6 cut(s) 268, 365, 510, 573, 927, 1188
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 830
GluI GCNGC 6 cut(s) 268, 365, 510, 573, 927, 1188
GsuI CTGGAG 1 cut(s) 1308
HaeIII GGCC 3 cut(s) 237, 752, 1280
HapII CCGG 3 cut(s) 153, 234, 560
HgaI GACGC 1 cut(s) 268
HhaI GCGC 1 cut(s) 831
Hin1I GRCGYC 2 cut(s) 194, 279
Hin1II CATG 8 cut(s) 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
Hin6I GCGC 1 cut(s) 829
HinP1I GCGC 1 cut(s) 829
HincII GTYRAC 2 cut(s) 480, 628
HindII GTYRAC 2 cut(s) 480, 628
HinfI GANTC 4 cut(s) 146, 293, 865, 1247
HpaI GTTAAC 1 cut(s) 480
HpaII CCGG 3 cut(s) 153, 234, 560
HphI GGTGA 6 cut(s) 74, 988, 997, 1106, 1114, 1189
Hpy166II GTNNAC 4 cut(s) 262, 480, 499, 628
Hpy188I TCNGA 4 cut(s) 174, 322, 349, 1209
Hpy188III TCNNGA 7 cut(s) 98, 190, 560, 693, 1034, 1046, 1063
Hpy8I GTNNAC 4 cut(s) 262, 480, 499, 628
HpyAV CCTTC 1 cut(s) 747
HpyCH4III ACNGT 2 cut(s) 428, 850
HpyCH4IV ACGT 2 cut(s) 194, 1357
HpyCH4V TGCA 8 cut(s) 267, 470, 530, 572, 935, 1088, 1128, 1172
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 276, 373, 515, 758, 932
HpyF3I CTNAG 2 cut(s) 171, 897
HpySE526I ACGT 2 cut(s) 194, 1357
Hsp92I GRCGYC 2 cut(s) 194, 279
Hsp92II CATG 8 cut(s) 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
HspAI GCGC 1 cut(s) 829
Kpn2I TCCGGA 1 cut(s) 559
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 480
Kzo9I GATC 5 cut(s) 21, 42, 100, 327, 689
LmnI GCTCC 1 cut(s) 115
Lsp1109I GCAGC 6 cut(s) 279, 351, 521, 584, 913, 1174
LweI GCATC 4 cut(s) 517, 584, 794, 1135
MaeI CTAG 1 cut(s) 377
MaeII ACGT 2 cut(s) 194, 1357
MaeIII GTNAC 3 cut(s) 385, 643, 1102
MalI GATC 5 cut(s) 23, 44, 102, 329, 691
MboI GATC 5 cut(s) 21, 42, 100, 327, 689
MboII GAAGA 9 cut(s) 16, 65, 127, 130, 280, 550, 574, 713, 1283
MlsI TGGCCA 1 cut(s) 1280
MluCI AATT 6 cut(s) 104, 520, 913, 996, 1015, 1345
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 1 cut(s) 1256
MmeI TCCRAC 2 cut(s) 83, 965
Mox20I TGGCCA 1 cut(s) 1280
MroI TCCGGA 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 233
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 5 cut(s) 414, 479, 686, 1317, 1363
MslI CAYNNNNRTG 1 cut(s) 38
Msp20I TGGCCA 1 cut(s) 1280
MspI CCGG 3 cut(s) 153, 234, 560
MvnI CGCG 1 cut(s) 829
MwoI GCNNNNNNNGC 6 cut(s) 116, 276, 373, 515, 758, 932
NaeI GCCGGC 1 cut(s) 235
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 5 cut(s) 21, 42, 100, 327, 689
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 8 cut(s) 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
NlaIV GGNNCC 2 cut(s) 157, 558
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 1102
NspI RCATGY 1 cut(s) 905
NspV TTCGAA 1 cut(s) 291
PaeI GCATGC 1 cut(s) 905
PagI TCATGA 1 cut(s) 1062
PaqCI CACCTGC 1 cut(s) 973
PceI AGGCCT 1 cut(s) 752
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 3 cut(s) 146, 293, 865
PflMI CCANNNNNTGG 1 cut(s) 973
PkrI GCNGC 6 cut(s) 269, 366, 511, 574, 928, 1189
PleI GAGTC 1 cut(s) 1255
PpsI GAGTC 1 cut(s) 1255
PspN4I GGNNCC 2 cut(s) 157, 558
PspPI GGNCC 1 cut(s) 557
PstI CTGCAG 2 cut(s) 269, 574
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
RseI CAYNNNNRTG 1 cut(s) 38
SaqAI TTAA 5 cut(s) 414, 479, 686, 1317, 1363
SatI GCNGC 6 cut(s) 268, 365, 510, 573, 927, 1188
Sau3AI GATC 5 cut(s) 21, 42, 100, 327, 689
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 1 cut(s) 1256
SfaNI GCATC 4 cut(s) 517, 584, 794, 1135
SfcI CTRYAG 3 cut(s) 265, 370, 570
SfuI TTCGAA 1 cut(s) 291
SinI GGWCC 1 cut(s) 557
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 188, 513
SmoI CTYRAG 2 cut(s) 188, 513
SphI GCATGC 1 cut(s) 905
Sse9I AATT 6 cut(s) 104, 520, 913, 996, 1015, 1345
SseBI AGGCCT 1 cut(s) 752
SsiI CCGC 1 cut(s) 871
SspI AATATT 2 cut(s) 210, 547
SspMI CTAG 1 cut(s) 377
StuI AGGCCT 1 cut(s) 752
StyI CCWWGG 3 cut(s) 274, 400, 1191
TaaI ACNGT 2 cut(s) 428, 850
TaiI ACGT 2 cut(s) 197, 1360
TasI AATT 6 cut(s) 104, 520, 913, 996, 1015, 1345
TfiI GAWTC 3 cut(s) 146, 293, 865
Tru1I TTAA 5 cut(s) 414, 479, 686, 1317, 1363
Tru9I TTAA 5 cut(s) 414, 479, 686, 1317, 1363
TscAI CASTG 2 cut(s) 269, 937
TseFI GTSAC 1 cut(s) 1102
TseI GCWGC 6 cut(s) 267, 364, 509, 572, 926, 1187
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 7 cut(s) 17, 714, 725, 878, 1051, 1207, 1294
TspRI CASTG 2 cut(s) 269, 937
Van91I CCANNNNNTGG 1 cut(s) 973
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 1 cut(s) 1345
XceI RCATGY 1 cut(s) 905
XspI CTAG 1 cut(s) 377
ZraI GACGTC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.