RchiOBHm_Chr4g0431791

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008131
Physical Location & Seq
Reverse (-)
55938775 .. 55940526
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40043

Sequence Viewer

Length: 1320 bp
ATGAAGATTGAAGTTGAAGTAATCTCTCAAGAAACAATAAAACCTTCAACTCCAACCCCTCACCATCTCCGCCATCACCAGCTCTCCTTTCTCGACCAGTTAGCTCCCCACGCCTACGTCCCTTTCCTCTACTTCTACTCCCTCCCCGATACTCACAACAACCACACCCAAATCTCCACCCACCTCAAGAAATCTCTCTCCAAAATCCTAACCCAGTACTACCCAATAGCCGGCCGAGTCAAATTCAACCTCTTTGTGGATTGCAACGACCATGGAATCCCCTTCTACGAAGCTCGAGTCAAATCCCACTCACTCTCTGATGTCATCACCAATCCCTCCCTCCCCGAATTGAACAAGTTGTTACCTTTCGAGCTCGACGTGGTCACAGACATAGCTCTCGGAGTTCAACTGAATCTGTTTCCGTGCGGAGGAATTGCCATTGGGTTGTGCGCTTCGCATCGAATTGCAGATGCGTTGTCATTTTTCACGTTTGTCAAGAACTGGGCATCAACTTCTTGTGGAAATGAGTGTGTGAATCCGGATTTTTCTGCAGCTTCGATCTTCCCTCCAAAGAATATGGATGGGTATGACGGCGTTCCTCTCCCGAAAAGGAAAGATATTGTGACCAAGAGGTTTGTGTTTGATGGGTCGAAGATCGAAGCTTTACGAGCAACGTATGCAGAGAGCATCAGACTGAAGACCAAGAAACGCCCGTCTCGGGTTGAGGCATTGTCTGCCTTCTCGTGGAACAGGTTTATGGCCGAGGTTGCAAAAGAAGAAGAATCAAGTTCAAGAAGGTTGTACACGGTTGTGTATATTATGGATCTCCGTTCGAGGTTTGACTCACCTCTGCCGCAGAATAGTTTTGGGAACTATTACCGGGCAGCCACTGCAACTCCAACATTGGTTGATGGGGAGGAGAGAGGTGGCCTTGTGAGGCAGGTGATTGAGGAAATAGAGAAGATTGATAGTAAGTATATGAGGAAATTTCAAGATGGCTATAAGGAACATTTGGATTTCATGAGGAGAAGGATGGAAAGGGCTGCAAAAGGAGAGCTTGTTACAGTGACGTTTTCAAGTCTGTGCAGGTTTCCATTGTATGAGGCTGATTTTGGTTGGGGGAAGCCAGCGTGGGTGAGCATGGCTTCCATGAGAATCAACAACCAAATAGTTTTTATGGACACCAAATTGGGTGATGGAATAGAGTCGTATTTTAGCTTCAAGGAGGAAGACATGGCCAAGTTTGAACTTGACTCAGAGTTCCTCGGGTTGATTTCTCCAATTGGTAATGTCAAGAAAAATCCATTTGCACGTCTTTAA

Protein Analysis

439

Amino Acids

49.99

Weight (kDa)

8.35

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 421 3.1e-80 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 931
Acc36I ACCTGC 2 cut(s) 931, 1077
AccIII TCCGGA 1 cut(s) 538
AciI CCGC 3 cut(s) 70, 426, 854
AclWI GGATC 1 cut(s) 831
AcoI YGGCCR 3 cut(s) 232, 759, 1236
AcsI RAATTY 2 cut(s) 242, 986
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 2 cut(s) 218, 803
AfiI CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
AjiI CACGTC 2 cut(s) 379, 1313
AleI CACNNNNGTG 1 cut(s) 809
AluBI AGCT 9 cut(s) 82, 104, 293, 373, 395, 554, 662, 1057, 1218
AluI AGCT 9 cut(s) 82, 104, 293, 373, 395, 554, 662, 1057, 1218
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 720
AlwI GGATC 1 cut(s) 831
AlwNI CAGNNNCTG 1 cut(s) 890
Ama87I CYCGRG 3 cut(s) 294, 717, 1265
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 4 cut(s) 232, 759, 928, 1236
ApeKI GCWGC 3 cut(s) 551, 884, 1043
ApoI RAATTY 2 cut(s) 242, 986
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspLEI GCGC 1 cut(s) 452
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 7 cut(s) 53, 68, 319, 837, 955, 1147, 1205
AvaI CYCGRG 3 cut(s) 294, 717, 1265
BalI TGGCCA 1 cut(s) 1238
BanII GRGCYC 1 cut(s) 375
BarI GAAGNNNNNNTAC 2 cut(s) 1202, 1234
BauI CACGAG 1 cut(s) 742
BbsI GAAGAC 2 cut(s) 704, 1236
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 563, 896, 1030
BccI CCATC 8 cut(s) 72, 81, 575, 638, 905, 989, 1027, 1190
BceAI ACGGC 1 cut(s) 607
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 1 cut(s) 720
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 2 cut(s) 931, 1077
BisI GCNGC 4 cut(s) 552, 854, 885, 1044
BlsI GCNGC 4 cut(s) 553, 855, 886, 1045
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 881
BmeT110I CYCGRG 3 cut(s) 294, 717, 1265
BmgBI CACGTC 2 cut(s) 379, 1313
BmrFI CCNGG 1 cut(s) 881
BmrI ACTGGG 2 cut(s) 208, 511
BmsI GCATC 4 cut(s) 460, 466, 515, 696
BmuI ACTGGG 2 cut(s) 208, 511
BpiI GAAGAC 2 cut(s) 704, 1236
BpuEI CTTGAG 2 cut(s) 12, 170
BpuMI CCSGG 1 cut(s) 881
BsaJI CCNNGG 3 cut(s) 271, 762, 1264
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 122, 152, 880, 910, 1044, 1074
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 3 cut(s) 97, 214, 506
BseAI TCCGGA 1 cut(s) 538
BseDI CCNNGG 3 cut(s) 271, 762, 1264
BseGI GGATG 2 cut(s) 586, 1038
BseLI CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
BseMII CTCAG 1 cut(s) 1269
BseNI ACTGG 3 cut(s) 97, 214, 506
BseRI GAGGAG 2 cut(s) 932, 1039
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 3 cut(s) 563, 896, 1030
BsgI GTGCAG 1 cut(s) 1105
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 4 cut(s) 234, 761, 930, 1238
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 3 cut(s) 294, 717, 1265
BsiSI CCGG 3 cut(s) 231, 539, 880
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
BsmAI GTCTC 1 cut(s) 720
BsmBI CGTCTC 1 cut(s) 720
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 4 cut(s) 234, 761, 930, 1238
BsoBI CYCGRG 3 cut(s) 294, 717, 1265
Bsp1286I GDGCHC 1 cut(s) 375
Bsp13I TCCGGA 1 cut(s) 538
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 3 cut(s) 558, 654, 823
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 3 cut(s) 70, 426, 854
BspANI GGCC 4 cut(s) 234, 761, 930, 1238
BspCNI CTCAG 1 cut(s) 1268
BspEI TCCGGA 1 cut(s) 538
BspHI TCATGA 1 cut(s) 1020
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 2 cut(s) 931, 1077
BspPI GGATC 1 cut(s) 831
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 3 cut(s) 97, 214, 506
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 271, 762, 1264
BssMI GATC 3 cut(s) 558, 654, 823
BssSI CACGAG 1 cut(s) 742
BssT1I CCWWGG 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 742
Bst4CI ACNGT 2 cut(s) 808, 1066
BstAPI GCANNNNNTGC 3 cut(s) 677, 734, 890
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 232, 1128
BstDEI CTNAG 1 cut(s) 1255
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 586, 1038
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 3 cut(s) 561, 657, 826
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 3 cut(s) 558, 654, 823
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 6 cut(s) 110, 668, 677, 734, 767, 890
BstSCI CCNGG 1 cut(s) 879
BstSFI CTRYAG 1 cut(s) 549
BstV1I GCAGC 3 cut(s) 563, 896, 1030
BstV2I GAAGAC 2 cut(s) 704, 1236
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 4 cut(s) 234, 761, 930, 1238
BtgI CCRYGG 1 cut(s) 271
BtrI CACGTC 2 cut(s) 379, 1313
BtsCI GGATG 2 cut(s) 586, 1038
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 2 cut(s) 888, 1071
BveI ACCTGC 2 cut(s) 931, 1077
Cac8I GCNNGC 2 cut(s) 232, 1128
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 1 cut(s) 452
Cfr10I RCCGGY 1 cut(s) 230
Csp6I GTAC 2 cut(s) 217, 802
CviAII CATG 5 cut(s) 272, 1021, 1141, 1150, 1234
CviQI GTAC 2 cut(s) 217, 802
DdeI CTNAG 1 cut(s) 1255
DpnI GATC 3 cut(s) 560, 656, 825
DpnII GATC 3 cut(s) 558, 654, 823
EaeI YGGCCR 3 cut(s) 232, 759, 1236
EagI CGGCCG 1 cut(s) 232
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 373
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 375
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 716
Eco88I CYCGRG 3 cut(s) 294, 717, 1265
EcoICRI GAGCTC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 720
FaeI CATG 5 cut(s) 275, 1024, 1144, 1153, 1237
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 1 cut(s) 104
FatI CATG 5 cut(s) 271, 1020, 1140, 1149, 1233
Fnu4HI GCNGC 4 cut(s) 552, 854, 885, 1044
FokI GGATG 2 cut(s) 593, 1045
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 4 cut(s) 552, 854, 885, 1044
GlaI GCGC 1 cut(s) 451
GluI GCNGC 4 cut(s) 552, 854, 885, 1044
HaeIII GGCC 4 cut(s) 234, 761, 930, 1238
HapII CCGG 3 cut(s) 231, 539, 880
HhaI GCGC 1 cut(s) 452
Hin1II CATG 5 cut(s) 275, 1024, 1144, 1153, 1237
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HindIII AAGCTT 1 cut(s) 660
HpaII CCGG 3 cut(s) 231, 539, 880
HphI GGTGA 7 cut(s) 53, 68, 319, 837, 955, 1147, 1205
Hpy166II GTNNAC 1 cut(s) 804
Hpy188I TCNGA 4 cut(s) 319, 401, 692, 1258
Hpy8I GTNNAC 1 cut(s) 804
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 5 cut(s) 54, 292, 748, 789, 1023
HpyCH4III ACNGT 2 cut(s) 808, 1066
HpyCH4IV ACGT 6 cut(s) 117, 378, 488, 674, 1070, 1312
HpyCH4V TGCA 9 cut(s) 264, 467, 551, 680, 770, 893, 1046, 1086, 1310
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 668, 677, 734, 767, 890
HpyF3I CTNAG 1 cut(s) 1255
HpySE526I ACGT 6 cut(s) 117, 378, 488, 674, 1070, 1312
Hsp92II CATG 5 cut(s) 275, 1024, 1144, 1153, 1237
HspAI GCGC 1 cut(s) 450
Kpn2I TCCGGA 1 cut(s) 538
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 3 cut(s) 558, 654, 823
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 3 cut(s) 563, 896, 1030
LweI GCATC 4 cut(s) 460, 466, 515, 696
MaeII ACGT 6 cut(s) 117, 378, 488, 674, 1070, 1312
MaeIII GTNAC 5 cut(s) 360, 382, 622, 1060, 1066
MalI GATC 3 cut(s) 560, 656, 825
MboI GATC 3 cut(s) 558, 654, 823
MboII GAAGA 8 cut(s) 16, 553, 664, 709, 788, 791, 973, 1241
MfeI CAATTG 1 cut(s) 1281
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 1 cut(s) 375
MlsI TGGCCA 1 cut(s) 1238
MluCI AATT 7 cut(s) 242, 347, 432, 462, 986, 1187, 1281
MluNI TGGCCA 1 cut(s) 1238
MlyI GAGTC 5 cut(s) 246, 306, 836, 1214, 1247
MmeI TCCRAC 2 cut(s) 77, 923
Mox20I TGGCCA 1 cut(s) 1238
MroI TCCGGA 1 cut(s) 538
MroNI GCCGGC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 1238
MseI TTAA 1 cut(s) 1318
MslI CAYNNNNRTG 1 cut(s) 809
Msp20I TGGCCA 1 cut(s) 1238
MspI CCGG 3 cut(s) 231, 539, 880
MspR9I CCNGG 1 cut(s) 881
MunI CAATTG 1 cut(s) 1281
MwoI GCNNNNNNNGC 6 cut(s) 110, 668, 677, 734, 767, 890
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 881
NcoI CCATGG 1 cut(s) 271
NdeII GATC 3 cut(s) 558, 654, 823
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 5 cut(s) 275, 1024, 1144, 1153, 1237
NmeAIII GCCGAG 2 cut(s) 260, 787
NmuCI GTSAC 3 cut(s) 382, 622, 1066
OliI CACNNNNGTG 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 294
PagI TCATGA 1 cut(s) 1020
PaqCI CACCTGC 1 cut(s) 931
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 5 cut(s) 276, 412, 535, 782, 1155
PflFI GACNNNGTC 1 cut(s) 380
PkrI GCNGC 4 cut(s) 553, 855, 886, 1045
PleI GAGTC 5 cut(s) 245, 305, 836, 1213, 1247
PpsI GAGTC 5 cut(s) 245, 305, 836, 1213, 1247
Psp124BI GAGCTC 1 cut(s) 375
PspXI VCTCGAGB 1 cut(s) 294
PstI CTGCAG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 1 cut(s) 823
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 2 cut(s) 218, 803
RsaNI GTAC 2 cut(s) 217, 802
RseI CAYNNNNRTG 1 cut(s) 809
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 1318
SatI GCNGC 4 cut(s) 552, 854, 885, 1044
Sau3AI GATC 3 cut(s) 558, 654, 823
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 5 cut(s) 246, 306, 836, 1214, 1247
ScrFI CCNGG 1 cut(s) 881
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 4 cut(s) 460, 466, 515, 696
SfcI CTRYAG 1 cut(s) 549
Sfr274I CTCGAG 1 cut(s) 294
SlaI CTCGAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 809
SmlI CTYRAG 3 cut(s) 27, 185, 294
SmoI CTYRAG 3 cut(s) 27, 185, 294
Sse9I AATT 7 cut(s) 242, 347, 432, 462, 986, 1187, 1281
SsiI CCGC 3 cut(s) 70, 426, 854
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 879
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 808, 1066
TaiI ACGT 6 cut(s) 120, 381, 491, 677, 1073, 1315
TaqI TCGA 9 cut(s) 93, 295, 369, 375, 460, 557, 650, 657, 833
TasI AATT 7 cut(s) 242, 347, 432, 462, 986, 1187, 1281
TatI WGTACW 2 cut(s) 216, 801
TauI GCSGC 1 cut(s) 856
TfiI GAWTC 5 cut(s) 276, 412, 535, 782, 1155
Tru1I TTAA 1 cut(s) 1318
Tru9I TTAA 1 cut(s) 1318
TscAI CASTG 2 cut(s) 895, 1071
TseFI GTSAC 3 cut(s) 382, 622, 1066
TseI GCWGC 3 cut(s) 551, 884, 1043
Tsp45I GTSAC 3 cut(s) 382, 622, 1066
TspDTI ATGAA 2 cut(s) 17, 1009
TspGWI ACGGA 2 cut(s) 411, 818
TspRI CASTG 2 cut(s) 895, 1071
Tth111I GACNNNGTC 1 cut(s) 380
XapI RAATTY 2 cut(s) 242, 986
XhoI CTCGAG 1 cut(s) 294
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.