MD16G1109100.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008131
Physical Location & Seq
Forward (+)
7643896 .. 7645200
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1109100.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGAGAATGGAGATTGAAGTTGAAGTAATATCCAAAGAGATCATCAAACCATCTTCTCCAACCCCAAACCATCTCCACCATCACCAGCTCTCGTTTCTCGATCAATTAGCTCCCCATGCATACATGCAATTTTTCTACTTTTACAGCTCCGACGGCGACGGCACCAAAACCTTCACTCAAATATCAAACATCCTCAAGACTTCTTTGTCCAAAGTCTTAACCCATTACTACCCGTTAGCCGGCCGAGTCAAACACAATCTCGTTGTTCACTGCAGTGACCAAGGCGTCCCATTCTTTGAAGCCAAAGTTAAATCCCAACTCCTTTCCGATGTGATCGCCAACCCTCTTCTCTCTGAGTCTGACCTGAACAGATTCTTGCCCTTCAAGACGGCTGAAGCTACTGAAATACCCCTCGGTGTTCAACTCAGTGTGTTTGATTGTGGAGGATTTGCAATTGGCGTTTGTATTTCGCATAGGATTGCTGATGCGTTGTCATATTTTACATTCATCAACAATTGGGCAGCTATTGCTAAGAATTGTAGCAATATTTTTGTTTGTCCAGATTTTTCTGCAGCCTCAGTTTTCCCACCAAGAAATGTGGAAGGGTATTTAGGGGTTTCTGTCATCACCAAAAGGAAAGCAATCATAACCAGGAGGTTTGTGTTTGATGGGGCAAAGGTTGAGGCTTTAAGATCGAGATACCAAGAGAGCATGGAGTTTTCAAAAACCCATAAACGCCCTTCAAGAGTTGAGGCCTTATCAGCTTTCTTGTGGAACATCTTTCTGCCATCAAGGCCGAGAGAACCTCTTCAAACTAGTCAAACTCTGTACACAGCTGTTTGCATTATGAATCTGCGGTCAAGATGTGATCCACCAGTGTCTCAATATGCTTTTGGGAATTATTACAGGGCTGCCACTGCAACTCCAACGTCGGTTAATGGGGAGGAGCGCCACCGCCTTGTGAGGCAGGCGATGGAGGAAATTGAGAAAATTGACAATAGTTACATGAGGAGATTTCAAGAGGGATATCAAGAACATTTGGATTTCATGAGGAAAAGAATGGAGAGGGCCGCAATAGGAGAGCTCGTGACATTGACCTTTTCGAGTTTGTGCAGATCTCCCATGTATCATGCTGATTTTGGTTGGGGGAAGCCTGCATGGGTGAGCTTGGCTGCCATGGGCATCACTAATCAGATAGTTTTCATGGACACCAAAAATGGTGATGGAATCGAGTCATATTATAGCTTGACGGAGGAAGACATGGCCAAGTTTGAACTTCACTGCGAGTTCATCTCGTTGCTTTAG

Protein Analysis

435

Amino Acids

49.36

Weight (kDa)

7.63

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 430 4.1e-82 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 205, 284
AccB1I GGYRCC 1 cut(s) 161
AciI CCGC 3 cut(s) 856, 955, 1071
AclWI GGATC 1 cut(s) 863
AcoI YGGCCR 2 cut(s) 241, 1263
AcuI CTGAAG 1 cut(s) 414
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 830
AfiI CCNNNNNNNGG 1 cut(s) 239
AhlI ACTAGT 1 cut(s) 815
AjnI CCWGG 1 cut(s) 650
AleI CACNNNNGTG 1 cut(s) 273
Alw21I GWGCWC 1 cut(s) 1086
Alw26I GTCTC 1 cut(s) 885
AlwI GGATC 1 cut(s) 863
AoxI GGCC 5 cut(s) 241, 753, 794, 1068, 1263
ApeKI GCWGC 4 cut(s) 521, 572, 911, 1172
Asp700I GAANNNNTTC 2 cut(s) 371, 807
AspLEI GCGC 1 cut(s) 951
AspS9I GGNCC 1 cut(s) 1068
AsuHPI GGTGA 4 cut(s) 74, 619, 1174, 1232
BalI TGGCCA 1 cut(s) 1265
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 1086
BauI CACGAG 1 cut(s) 1085
BbsI GAAGAC 1 cut(s) 1263
Bbv12I GWGCWC 1 cut(s) 1086
BbvI GCAGC 4 cut(s) 533, 584, 898, 1159
BccI CCATC 7 cut(s) 58, 78, 87, 662, 796, 967, 1217
BceAI ACGGC 3 cut(s) 169, 175, 405
BciT130I CCWGG 1 cut(s) 652
BcoDI GTCTC 1 cut(s) 885
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 1 cut(s) 816
BfmI CTRYAG 2 cut(s) 271, 570
BfoI RGCGCY 1 cut(s) 952
BglI GCCNNNNNGGC 1 cut(s) 793
BglII AGATCT 1 cut(s) 1115
BisI GCNGC 5 cut(s) 522, 573, 912, 1071, 1173
BlsI GCNGC 5 cut(s) 523, 574, 913, 1072, 1174
Bme1390I CCNGG 1 cut(s) 652
BmgT120I GGNCC 1 cut(s) 1068
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 1 cut(s) 652
BmsI GCATC 2 cut(s) 475, 1191
BpiI GAAGAC 1 cut(s) 1263
BplI GAGNNNNNCTC 3 cut(s) 790, 822, 1277
BpuEI CTTGAG 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 280, 412, 1176
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 1071, 1101
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 875
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 3 cut(s) 280, 412, 1176
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 3 cut(s) 345, 439, 591
BseNI ACTGG 1 cut(s) 875
BseRI GAGGAG 2 cut(s) 959, 1024
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 4 cut(s) 533, 584, 898, 1159
BsgI GTGCAG 1 cut(s) 1132
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 5 cut(s) 243, 755, 796, 1070, 1265
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 1086
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 885
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 5 cut(s) 243, 755, 796, 1070, 1265
Bsp1286I GDGCHC 1 cut(s) 1086
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
Bsp19I CCATGG 1 cut(s) 1176
BspACI CCGC 3 cut(s) 856, 955, 1071
BspANI GGCC 5 cut(s) 243, 755, 796, 1070, 1265
BspCNI CTCAG 3 cut(s) 346, 438, 590
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 2 cut(s) 275, 574
BspPI GGATC 1 cut(s) 863
BspT107I GGYRCC 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 239
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 1 cut(s) 875
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 3 cut(s) 280, 412, 1176
BssMI GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
BssNI GRCGYC 1 cut(s) 285
BssSI CACGAG 1 cut(s) 1085
BssT1I CCWWGG 2 cut(s) 280, 1176
Bst2BI CACGAG 1 cut(s) 1085
Bst2UI CCWGG 1 cut(s) 652
Bst6I CTCTTC 2 cut(s) 351, 813
BstACI GRCGYC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 527
BstAUI TGTACA 1 cut(s) 828
BstC8I GCNNGC 3 cut(s) 241, 969, 1155
BstDEI CTNAG 4 cut(s) 354, 425, 531, 577
BstDSI CCRYGG 1 cut(s) 1176
BstF5I GGATG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 952
BstHHI GCGC 1 cut(s) 951
BstKTI GATC 6 cut(s) 42, 103, 336, 695, 871, 1118
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 6 cut(s) 116, 153, 527, 761, 793, 917
BstNI CCWGG 1 cut(s) 652
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 650
BstSFI CTRYAG 2 cut(s) 271, 570
BstV1I GCAGC 4 cut(s) 533, 584, 898, 1159
BstV2I GAAGAC 1 cut(s) 1263
BstX2I RGATCY 1 cut(s) 1115
BstYI RGATCY 1 cut(s) 1115
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 5 cut(s) 243, 755, 796, 1070, 1265
BtgI CCRYGG 1 cut(s) 1176
BtgZI GCGATG 1 cut(s) 986
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 4 cut(s) 268, 280, 915, 1279
BtsIMutI CAGTG 6 cut(s) 268, 280, 433, 882, 915, 1279
Cac8I GCNNGC 3 cut(s) 241, 969, 1155
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 1 cut(s) 951
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 1068
CseI GACGC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 829
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviQI GTAC 1 cut(s) 829
DdeI CTNAG 4 cut(s) 354, 425, 531, 577
DpnI GATC 6 cut(s) 41, 102, 335, 694, 870, 1117
DpnII GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
DrdI GACNNNNNNGTC 2 cut(s) 205, 284
DseDI GACNNNNNNGTC 2 cut(s) 205, 284
EaeI YGGCCR 2 cut(s) 241, 1263
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 351, 813
EarI CTCTTC 2 cut(s) 351, 813
Ecl136II GAGCTC 1 cut(s) 1084
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 280, 1176
Eco147I AGGCCT 1 cut(s) 755
Eco24I GRGCYC 1 cut(s) 1086
Eco32I GATATC 1 cut(s) 1028
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 1084
Eco57I CTGAAG 1 cut(s) 414
EcoICRI GAGCTC 1 cut(s) 1084
EcoRII CCWGG 1 cut(s) 650
EcoRV GATATC 1 cut(s) 1028
EcoT14I CCWWGG 2 cut(s) 280, 1176
EcoT22I ATGCAT 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 1086
ErhI CCWWGG 2 cut(s) 280, 1176
FaqI GGGAC 1 cut(s) 272
Fnu4HI GCNGC 5 cut(s) 522, 573, 912, 1071, 1173
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 1086
Fsp4HI GCNGC 5 cut(s) 522, 573, 912, 1071, 1173
FspBI CTAG 1 cut(s) 816
GlaI GCGC 1 cut(s) 950
GluI GCNGC 5 cut(s) 522, 573, 912, 1071, 1173
HaeII RGCGCY 1 cut(s) 952
HaeIII GGCC 5 cut(s) 243, 755, 796, 1070, 1265
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 951
Hin1I GRCGYC 1 cut(s) 285
Hin6I GCGC 1 cut(s) 949
HinP1I GCGC 1 cut(s) 949
HinfI GANTC 6 cut(s) 246, 356, 372, 850, 1227, 1232
HpaII CCGG 1 cut(s) 240
HphI GGTGA 4 cut(s) 74, 619, 1174, 1232
Hpy166II GTNNAC 2 cut(s) 268, 831
Hpy188I TCNGA 5 cut(s) 151, 328, 355, 361, 1194
Hpy8I GTNNAC 2 cut(s) 268, 831
Hpy99I CGWCG 3 cut(s) 155, 161, 934
HpyAV CCTTC 4 cut(s) 181, 391, 596, 750
HpyCH4IV ACGT 1 cut(s) 929
HpyCH4V TGCA 9 cut(s) 119, 127, 273, 452, 572, 843, 920, 1113, 1157
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 153, 527, 761, 793, 917
HpyF3I CTNAG 4 cut(s) 354, 425, 531, 577
HpySE526I ACGT 1 cut(s) 929
Hsp92I GRCGYC 1 cut(s) 285
HspAI GCGC 1 cut(s) 949
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
LmnI GCTCC 3 cut(s) 115, 152, 946
Lsp1109I GCAGC 4 cut(s) 533, 584, 898, 1159
LweI GCATC 2 cut(s) 475, 1191
MaeI CTAG 1 cut(s) 816
MaeII ACGT 1 cut(s) 929
MaeIII GTNAC 3 cut(s) 275, 1001, 1087
MalI GATC 6 cut(s) 41, 102, 335, 694, 870, 1117
MboI GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
MboII GAAGA 4 cut(s) 45, 338, 800, 1268
MfeI CAATTG 2 cut(s) 453, 514
MflI RGATCY 1 cut(s) 1115
MhlI GDGCHC 1 cut(s) 1086
MlsI TGGCCA 1 cut(s) 1265
MluCI AATT 8 cut(s) 104, 128, 453, 514, 535, 898, 981, 990
MluNI TGGCCA 1 cut(s) 1265
MlyI GAGTC 3 cut(s) 255, 365, 1241
MmeI TCCRAC 3 cut(s) 83, 174, 950
Mox20I TGGCCA 1 cut(s) 1265
Mph1103I ATGCAT 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 2 cut(s) 371, 807
MscI TGGCCA 1 cut(s) 1265
MseI TTAA 4 cut(s) 218, 309, 689, 936
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 1265
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 1 cut(s) 652
MunI CAATTG 2 cut(s) 453, 514
MvaI CCWGG 1 cut(s) 652
MwoI GCNNNNNNNGC 6 cut(s) 116, 153, 527, 761, 793, 917
NaeI GCCGGC 1 cut(s) 241
NcoI CCATGG 1 cut(s) 1176
NdeII GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
NgoMIV GCCGGC 1 cut(s) 239
NlaIV GGNNCC 1 cut(s) 163
NmeAIII GCCGAG 2 cut(s) 269, 822
NmuCI GTSAC 2 cut(s) 275, 1087
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 127
OliI CACNNNNGTG 1 cut(s) 273
PagI TCATGA 1 cut(s) 1047
PceI AGGCCT 1 cut(s) 755
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 2 cut(s) 371, 807
PfeI GAWTC 3 cut(s) 372, 850, 1227
PkrI GCNGC 5 cut(s) 523, 574, 913, 1072, 1174
PleI GAGTC 3 cut(s) 254, 364, 1240
PpsI GAGTC 3 cut(s) 254, 364, 1240
Psp124BI GAGCTC 1 cut(s) 1086
Psp6I CCWGG 1 cut(s) 650
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 1068
PstI CTGCAG 2 cut(s) 275, 574
PsuI RGATCY 1 cut(s) 1115
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 1 cut(s) 830
RsaNI GTAC 1 cut(s) 829
RseI CAYNNNNRTG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 1086
SaqAI TTAA 4 cut(s) 218, 309, 689, 936
SatI GCNGC 5 cut(s) 522, 573, 912, 1071, 1173
Sau3AI GATC 6 cut(s) 39, 100, 333, 692, 868, 1115
Sau96I GGNCC 1 cut(s) 1068
SchI GAGTC 3 cut(s) 255, 365, 1241
ScrFI CCNGG 1 cut(s) 652
SduI GDGCHC 1 cut(s) 1086
SfaNI GCATC 2 cut(s) 475, 1191
SfcI CTRYAG 2 cut(s) 271, 570
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 8 cut(s) 104, 128, 453, 514, 535, 898, 981, 990
SseBI AGGCCT 1 cut(s) 755
SsiI CCGC 3 cut(s) 856, 955, 1071
SspI AATATT 1 cut(s) 547
SspMI CTAG 1 cut(s) 816
SstI GAGCTC 1 cut(s) 1086
StuI AGGCCT 1 cut(s) 755
StyD4I CCNGG 1 cut(s) 650
StyI CCWWGG 2 cut(s) 280, 1176
TaiI ACGT 1 cut(s) 932
TaqI TCGA 4 cut(s) 99, 695, 1103, 1230
TasI AATT 8 cut(s) 104, 128, 453, 514, 535, 898, 981, 990
TatI WGTACW 1 cut(s) 828
TauI GCSGC 1 cut(s) 1073
TfiI GAWTC 3 cut(s) 372, 850, 1227
Tru1I TTAA 4 cut(s) 218, 309, 689, 936
Tru9I TTAA 4 cut(s) 218, 309, 689, 936
TscAI CASTG 6 cut(s) 275, 280, 433, 882, 922, 1286
TseFI GTSAC 2 cut(s) 275, 1087
TseI GCWGC 4 cut(s) 521, 572, 911, 1172
Tsp45I GTSAC 2 cut(s) 275, 1087
TspDTI ATGAA 5 cut(s) 496, 863, 1036, 1192, 1279
TspGWI ACGGA 1 cut(s) 1265
TspRI CASTG 6 cut(s) 275, 280, 433, 882, 922, 1286
XceI RCATGY 1 cut(s) 127
XmnI GAANNNNTTC 2 cut(s) 371, 807
XspI CTAG 1 cut(s) 816
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.