Rorug07G0003600

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008131
Physical Location & Seq
Reverse (-)
321611 .. 323700
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0003600.1

Sequence Viewer

Length: 1506 bp
ATGGAGATGCTTATCCTTTCTGTGCTAGTTCTCTTTGTTTCTCTCTACTATCTTTTTCGTATGTTGAATAGAAATGCTAGCAAAAATGAAGTTCATCATCATAATCATCAGCTTCAGCTCCCACCAGGAAGCTCAGGTTGGCCCATCATCGGGGAAGGCCTTGCTTTTTTGATGACAGCCAAAAGGGGTGTCCCGGAGAAGTTCATAGGAGAAAGGAGGATCAAGTACTCATCATCAGATTCCAAAAGCAGCAGCTGCAAGGTGTTCACCACATCGTTGCTGCGTGAATCCATGGCGGTGCTATGTACTGCCGCTGGAAACAAGTTCCTGTTTTCGAACGAGAACAAACTCGTCAAGTCATGGTGGCCTGCTAACATCGACACCATCTTTGCCAGCTCCAACAAAACAAACACCGCCGAGGAGTCCATTCGTCACCGCAAAGTTCTGTCTCCGTTTCTCGGCCCCGATTCTCTCCGAAAGTACGTAGGGATCATGGATACTGTCACCAAACGTCATCTGGACCTGTTCTGGTCGGATCAAAAACAAGTCAAAGTTCTAGCCCTAACAAAGATGCACACCTTCACATTGGTGTGTAAAATCTTCTTAAACGTGGAGGATCCAGCAGTGATTGCCAAACTAGAGGAGCCAATTCAGCGTATTGCAGCAGGCTTCATTTCATTGCCCATAAATCTGCCTGGCACAGCATTCAACCGAGCCATCCAAGCATCCAAGCAGGTCAGGAAGGACCTTGAAAATATGGTTAAACAGAGGAGAATAGATTTAGATCTGTCTGTGGCAGCAGATGATGAAAATAATAATAATCATGATTTGATGTCTAAGTTGCTGCTGGAGAGATATAGCGACGGCCAACAAATGAAGGAAACCGACATCGCCAACAAGTTAAATGGTCTACTCATTGCTGCTTATGACAATGTATTCATCACCTTGTGTTCTATCATCATGTATCTATCGGAGCTTCCTACAGTTTATGATGCCGTCCTTAAAGAGCAAATGGAGATAGCAGATTCAAAAGCAGAAGGAGAGTTGCTCAACTGGGAGGACATACAAAAAATGAAGTACACATGGAATGTCGCATGTGAAGTGTTGAGATTGGAGCCGCCAAATCCTGGAACTTTTAGAGAGGCCATGACCGACTTTGTCTATGAAGGATATCTGATTCCTAAAGGAATGAAGTTATACTGGAGTGTGTTTTCGACACATAAAAACCCAGAGTACTTCCCAGATCCGAAAAAGTTCGACCCATCAAGGTTCGACGGAGGAGGACCAGCTCCTTTTTCGTACGTCCCATTTGGAGGAGGACCTCGGATGTGCCCTGGAAGAGAATATGCTCGCTTTACGATTTTGGTTTTCATGCATAACTTGGTCACCAGATTCAGGTGGGAGAAGGTTTTTCCTGAAGAGAAAATGGTGATGACCCCATTTCTTCTTCCTACCGAAGGACTTCCAGTTCGCCTTTATCCTCATCCATCAACATCATCACTGTGA

Protein Analysis

501

Amino Acids

57.12

Weight (kDa)

8.65

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 65 - 485 4.7e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 724
AccB7I CCANNNNNTGG 1 cut(s) 1127
AccI GTMKAC 1 cut(s) 910
AciI CCGC 5 cut(s) 296, 312, 414, 436, 1118
AclWI GGATC 6 cut(s) 227, 497, 543, 611, 624, 1238
AcoI YGGCCR 1 cut(s) 865
AcuI CTGAAG 2 cut(s) 98, 1437
AdeI CACNNNGTG 1 cut(s) 948
AfaI GTAC 6 cut(s) 227, 307, 482, 1079, 1235, 1301
AfiI CCNNNNNNNGG 7 cut(s) 149, 150, 458, 1127, 1313, 1395, 1457
AgsI TTSAA 4 cut(s) 67, 709, 752, 1029
AjnI CCWGG 4 cut(s) 124, 694, 1126, 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 121, 153
AleI CACNNNNGTG 1 cut(s) 587
AloI GAACNNNNNNTCC 2 cut(s) 1452, 1484
AluBI AGCT 7 cut(s) 112, 118, 132, 255, 396, 976, 1289
AluI AGCT 7 cut(s) 112, 118, 132, 255, 396, 976, 1289
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 6 cut(s) 227, 497, 543, 611, 624, 1238
AlwNI CAGNNNCTG 1 cut(s) 255
AoxI GGCC 6 cut(s) 140, 157, 365, 460, 865, 1143
ApeKI GCWGC 8 cut(s) 249, 252, 255, 280, 662, 797, 844, 920
ArsI GACNNNNNNTTYG 6 cut(s) 174, 206, 371, 403, 1452, 1484
Asp700I GAANNNNTTC 2 cut(s) 1253, 1461
AspS9I GGNCC 6 cut(s) 141, 461, 520, 745, 1283, 1319
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 6 cut(s) 259, 425, 496, 934, 1378, 1441
AsuII TTCGAA 1 cut(s) 335
AsuNHI GCTAGC 1 cut(s) 77
AvaII GGWCC 4 cut(s) 520, 745, 1283, 1319
BaeGI GKGCMC 1 cut(s) 1334
BamHI GGATCC 1 cut(s) 616
BbvI GCAGC 8 cut(s) 242, 261, 264, 267, 674, 809, 831, 907
BccI CCATC 5 cut(s) 152, 392, 725, 1270, 1495
BceAI ACGGC 2 cut(s) 880, 980
BciT130I CCWGG 4 cut(s) 126, 696, 1128, 1335
BciVI GTATCC 1 cut(s) 490
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 4 cut(s) 26, 78, 557, 638
BfmI CTRYAG 1 cut(s) 981
BfuAI ACCTGC 1 cut(s) 724
BfuI GTATCC 1 cut(s) 490
BglII AGATCT 1 cut(s) 784
BmcAI AGTACT 2 cut(s) 227, 1235
Bme1390I CCNGG 5 cut(s) 126, 194, 696, 1128, 1335
Bme18I GGWCC 4 cut(s) 520, 745, 1283, 1319
BmgT120I GGNCC 6 cut(s) 141, 461, 520, 745, 1283, 1319
BmiI GGNNCC 4 cut(s) 463, 618, 645, 1116
BmrFI CCNGG 5 cut(s) 126, 194, 696, 1128, 1335
BmrI ACTGGG 1 cut(s) 1063
BmsI GCATC 3 cut(s) 561, 734, 982
BmtI GCTAGC 1 cut(s) 81
BmuI ACTGGG 1 cut(s) 1063
BplI GAGNNNNNCTC 2 cut(s) 1032, 1064
BpmI CTGGAG 2 cut(s) 869, 1222
Bpu10I CCTNAGC 1 cut(s) 133
Bpu14I TTCGAA 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 194
BsaAI YACGTR 1 cut(s) 484
BsaBI GATNNNNATC 2 cut(s) 11, 783
BsaJI CCNNGG 4 cut(s) 291, 417, 1322, 1333
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 150, 458, 1127, 1313, 1395, 1457
Bse1I ACTGG 3 cut(s) 1058, 1205, 1466
Bse3DI GCAATG 2 cut(s) 677, 915
Bse8I GATNNNNATC 2 cut(s) 11, 783
BseBI CCWGG 4 cut(s) 126, 696, 1128, 1335
BseDI CCNNGG 4 cut(s) 291, 417, 1322, 1333
BseGI GGATG 4 cut(s) 717, 725, 1332, 1483
BseJI GATNNNNATC 2 cut(s) 11, 783
BseLI CCNNNNNNNGG 7 cut(s) 149, 150, 458, 1127, 1313, 1395, 1457
BseMI GCAATG 2 cut(s) 677, 915
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 3 cut(s) 1058, 1205, 1466
BseRI GAGGAG 5 cut(s) 434, 656, 784, 1293, 1329
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 8 cut(s) 242, 261, 264, 267, 674, 809, 831, 907
BshFI GGCC 6 cut(s) 142, 159, 367, 462, 867, 1145
BsiSI CCGG 1 cut(s) 194
BsiWI CGTACG 1 cut(s) 1299
BslFI GGGAC 2 cut(s) 176, 1289
BslI CCNNNNNNNGG 7 cut(s) 149, 150, 458, 1127, 1313, 1395, 1457
BsmAI GTCTC 1 cut(s) 453
BsmFI GGGAC 2 cut(s) 176, 1289
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 6 cut(s) 142, 159, 367, 462, 867, 1145
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 1334
Bsp143I GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 5 cut(s) 296, 312, 414, 436, 1118
BspANI GGCC 6 cut(s) 142, 159, 367, 462, 867, 1145
BspCNI CTCAG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 4 cut(s) 463, 618, 645, 1116
BspMI ACCTGC 1 cut(s) 724
BspOI GCTAGC 1 cut(s) 81
BspPI GGATC 6 cut(s) 227, 497, 543, 611, 624, 1238
BspT104I TTCGAA 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 677, 915
BsrI ACTGG 3 cut(s) 1058, 1205, 1466
BssECI CCNNGG 4 cut(s) 291, 417, 1322, 1333
BssMI GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 4 cut(s) 126, 696, 1128, 1335
Bst4CI ACNGT 3 cut(s) 502, 985, 1503
Bst6I CTCTTC 2 cut(s) 1333, 1413
BstAPI GCANNNNNTGC 2 cut(s) 255, 629
BstBAI YACGTR 1 cut(s) 484
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 5 cut(s) 79, 369, 394, 667, 1351
BstDEI CTNAG 2 cut(s) 133, 837
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 1 cut(s) 1384
BstENI CCTNNNNNAGG 1 cut(s) 1455
BstF5I GGATG 4 cut(s) 717, 725, 1332, 1483
BstKTI GATC 6 cut(s) 222, 492, 538, 619, 787, 1246
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
BstMWI GCNNNNNNNGC 4 cut(s) 255, 629, 652, 722
BstNI CCWGG 4 cut(s) 126, 696, 1128, 1335
BstNSI RCATGY 1 cut(s) 1098
BstPI GGTNACC 1 cut(s) 1384
BstSCI CCNGG 5 cut(s) 124, 192, 694, 1126, 1333
BstSFI CTRYAG 1 cut(s) 981
BstSLI GKGCMC 1 cut(s) 1334
BstSNI TACGTA 1 cut(s) 484
BstV1I GCAGC 8 cut(s) 242, 261, 264, 267, 674, 809, 831, 907
BstX2I RGATCY 3 cut(s) 616, 784, 1243
BstYI RGATCY 3 cut(s) 616, 784, 1243
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 6 cut(s) 142, 159, 367, 462, 867, 1145
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 874
BtsCI GGATG 4 cut(s) 717, 725, 1332, 1483
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 2 cut(s) 630, 1499
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 5 cut(s) 79, 369, 394, 667, 1351
CaiI CAGNNNCTG 1 cut(s) 255
CciI TCATGA 1 cut(s) 823
Cfr13I GGNCC 6 cut(s) 141, 461, 520, 745, 1283, 1319
Csp6I GTAC 6 cut(s) 226, 306, 481, 1078, 1234, 1300
CviAII CATG 9 cut(s) 292, 360, 493, 824, 961, 1083, 1095, 1147, 1372
CviQI GTAC 6 cut(s) 226, 306, 481, 1078, 1234, 1300
DdeI CTNAG 2 cut(s) 133, 837
DpnI GATC 6 cut(s) 221, 491, 537, 618, 786, 1245
DpnII GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
DraIII CACNNNGTG 1 cut(s) 948
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 2 cut(s) 1333, 1413
EarI CTCTTC 2 cut(s) 1333, 1413
Eco105I TACGTA 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 291
Eco147I AGGCCT 1 cut(s) 159
Eco32I GATATC 1 cut(s) 1172
Eco47I GGWCC 4 cut(s) 520, 745, 1283, 1319
Eco57I CTGAAG 2 cut(s) 98, 1437
Eco91I GGTNACC 1 cut(s) 1384
EcoNI CCTNNNNNAGG 1 cut(s) 1455
EcoO109I RGGNCCY 2 cut(s) 745, 1319
EcoO65I GGTNACC 1 cut(s) 1384
EcoRII CCWGG 4 cut(s) 124, 694, 1126, 1333
EcoRV GATATC 1 cut(s) 1172
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 1377
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 9 cut(s) 295, 363, 496, 827, 964, 1086, 1098, 1150, 1375
FaqI GGGAC 2 cut(s) 176, 1289
FatI CATG 9 cut(s) 291, 359, 492, 823, 960, 1082, 1094, 1146, 1371
FblI GTMKAC 1 cut(s) 910
FokI GGATG 4 cut(s) 704, 712, 1339, 1470
FspBI CTAG 4 cut(s) 26, 78, 557, 638
GsuI CTGGAG 2 cut(s) 869, 1222
HaeIII GGCC 6 cut(s) 142, 159, 367, 462, 867, 1145
HapII CCGG 1 cut(s) 194
Hin1II CATG 9 cut(s) 295, 363, 496, 827, 964, 1086, 1098, 1150, 1375
HinfI GANTC 7 cut(s) 239, 287, 422, 467, 1025, 1177, 1392
HpaII CCGG 1 cut(s) 194
HphI GGTGA 6 cut(s) 259, 425, 496, 934, 1378, 1441
Hpy166II GTNNAC 3 cut(s) 267, 911, 1080
Hpy188I TCNGA 7 cut(s) 238, 476, 535, 973, 1176, 1248, 1326
Hpy188III TCNNGA 4 cut(s) 518, 739, 824, 1415
Hpy8I GTNNAC 3 cut(s) 267, 911, 1080
Hpy99I CGWCG 2 cut(s) 866, 1277
HpyAV CCTTC 8 cut(s) 149, 589, 736, 871, 1031, 1160, 1399, 1451
HpyCH4III ACNGT 3 cut(s) 502, 985, 1503
HpyCH4IV ACGT 4 cut(s) 483, 511, 609, 1302
HpyCH4V TGCA 4 cut(s) 258, 574, 662, 1375
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 629, 652, 722
HpyF3I CTNAG 2 cut(s) 133, 837
HpySE526I ACGT 4 cut(s) 483, 511, 609, 1302
Hsp92II CATG 9 cut(s) 295, 363, 496, 827, 964, 1086, 1098, 1150, 1375
Kzo9I GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
LmnI GCTCC 6 cut(s) 123, 401, 643, 973, 1114, 1294
Lsp1109I GCAGC 8 cut(s) 242, 261, 264, 267, 674, 809, 831, 907
LweI GCATC 3 cut(s) 561, 734, 982
MaeI CTAG 4 cut(s) 26, 78, 557, 638
MaeII ACGT 4 cut(s) 483, 511, 609, 1302
MaeIII GTNAC 3 cut(s) 431, 502, 1384
MalI GATC 6 cut(s) 221, 491, 537, 618, 786, 1245
MboI GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
MboII GAAGA 5 cut(s) 592, 1350, 1430, 1436, 1439
MflI RGATCY 3 cut(s) 616, 784, 1243
MhlI GDGCHC 1 cut(s) 1334
MluCI AATT 1 cut(s) 648
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 2 cut(s) 423, 513
Mph1103I ATGCAT 1 cut(s) 1377
MroXI GAANNNNTTC 2 cut(s) 1253, 1461
MseI TTAA 4 cut(s) 605, 762, 902, 1002
MslI CAYNNNNRTG 4 cut(s) 296, 587, 589, 1501
MspA1I CMGCKG 2 cut(s) 255, 314
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 5 cut(s) 126, 194, 696, 1128, 1335
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 4 cut(s) 126, 696, 1128, 1335
MwoI GCNNNNNNNGC 4 cut(s) 255, 629, 652, 722
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 291
NdeII GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 9 cut(s) 295, 363, 496, 827, 964, 1086, 1098, 1150, 1375
NlaIV GGNNCC 4 cut(s) 463, 618, 645, 1116
NmeAIII GCCGAG 2 cut(s) 438, 442
NmuCI GTSAC 3 cut(s) 431, 502, 1384
NsiI ATGCAT 1 cut(s) 1377
NspI RCATGY 1 cut(s) 1098
NspV TTCGAA 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 587
PagI TCATGA 1 cut(s) 823
PceI AGGCCT 1 cut(s) 159
PctI GAATGC 1 cut(s) 704
PdmI GAANNNNTTC 2 cut(s) 1253, 1461
PfeI GAWTC 6 cut(s) 239, 287, 467, 1025, 1177, 1392
Pfl23II CGTACG 1 cut(s) 1299
PflFI GACNNNGTC 1 cut(s) 1157
PflMI CCANNNNNTGG 1 cut(s) 1127
PfoI TCCNGGA 2 cut(s) 192, 1126
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 484
PpuMI RGGWCCY 2 cut(s) 745, 1319
Psp5II RGGWCCY 2 cut(s) 745, 1319
Psp6I CCWGG 4 cut(s) 124, 694, 1126, 1333
PspEI GGTNACC 1 cut(s) 1384
PspGI CCWGG 4 cut(s) 124, 694, 1126, 1333
PspLI CGTACG 1 cut(s) 1299
PspN4I GGNNCC 4 cut(s) 463, 618, 645, 1116
PspPI GGNCC 6 cut(s) 141, 461, 520, 745, 1283, 1319
PspPPI RGGWCCY 2 cut(s) 745, 1319
PstNI CAGNNNCTG 1 cut(s) 255
PsuI RGATCY 3 cut(s) 616, 784, 1243
PsyI GACNNNGTC 1 cut(s) 1157
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 6 cut(s) 227, 307, 482, 1079, 1235, 1301
RsaNI GTAC 6 cut(s) 226, 306, 481, 1078, 1234, 1300
RseI CAYNNNNRTG 4 cut(s) 296, 587, 589, 1501
SaqAI TTAA 4 cut(s) 605, 762, 902, 1002
Sau3AI GATC 6 cut(s) 219, 489, 535, 616, 784, 1243
Sau96I GGNCC 6 cut(s) 141, 461, 520, 745, 1283, 1319
ScaI AGTACT 2 cut(s) 227, 1235
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 5 cut(s) 126, 194, 696, 1128, 1335
SduI GDGCHC 1 cut(s) 1334
SfaNI GCATC 3 cut(s) 561, 734, 982
SfcI CTRYAG 1 cut(s) 981
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 4 cut(s) 520, 745, 1283, 1319
SmiMI CAYNNNNRTG 4 cut(s) 296, 587, 589, 1501
SnaBI TACGTA 1 cut(s) 484
Sse9I AATT 1 cut(s) 648
SseBI AGGCCT 1 cut(s) 159
SsiI CCGC 5 cut(s) 296, 312, 414, 436, 1118
SspMI CTAG 4 cut(s) 26, 78, 557, 638
StuI AGGCCT 1 cut(s) 159
StyD4I CCNGG 5 cut(s) 124, 192, 694, 1126, 1333
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 3 cut(s) 502, 985, 1503
TaiI ACGT 4 cut(s) 486, 514, 612, 1305
TaqI TCGA 5 cut(s) 335, 378, 1214, 1257, 1272
TaqII GACCGA 1 cut(s) 1166
TasI AATT 1 cut(s) 648
TatI WGTACW 4 cut(s) 225, 305, 1077, 1233
TauI GCSGC 2 cut(s) 314, 1120
TfiI GAWTC 6 cut(s) 239, 287, 467, 1025, 1177, 1392
Tru1I TTAA 4 cut(s) 605, 762, 902, 1002
Tru9I TTAA 4 cut(s) 605, 762, 902, 1002
TscAI CASTG 2 cut(s) 630, 1506
TseFI GTSAC 3 cut(s) 431, 502, 1384
TseI GCWGC 8 cut(s) 249, 252, 255, 280, 662, 797, 844, 920
Tsp45I GTSAC 3 cut(s) 431, 502, 1384
TspGWI ACGGA 2 cut(s) 441, 1290
TspRI CASTG 2 cut(s) 630, 1506
Tth111I GACNNNGTC 1 cut(s) 1157
Van91I CCANNNNNTGG 1 cut(s) 1127
VpaK11BI GGWCC 4 cut(s) 520, 745, 1283, 1319
XagI CCTNNNNNAGG 1 cut(s) 1455
XceI RCATGY 1 cut(s) 1098
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 910
XmnI GAANNNNTTC 2 cut(s) 1253, 1461
XspI CTAG 4 cut(s) 26, 78, 557, 638
ZrmI AGTACT 2 cut(s) 227, 1235
Zsp2I ATGCAT 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.