Rw5G016090

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008131
Physical Location & Seq
Forward (+)
20116307 .. 20120690
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016090.1

Sequence Viewer

Length: 1308 bp
ATGAAGGTTGAGGTTGAGGTTGTCTCCAGAGAGCTTATCAGACCATCTTCTCCAACCCCTGACCACCTCCACCGATACCAACTCTCCTTTCTTGACCAGATATCTCCCCCAGTCTTTATTCCTTTGGTTCTATTTTATTCATCATCCGAGGCTGATACAAATTTCGACATTACTGATATATCGAATCGGCTAAAGAAATCCCTCTCCGATGTCTTGGTCCAATTCTACCCACTCGCTGGACGAGCAAATGACGACGTGTTCATAGACTGCAACGACAAGGGCATACCCTTCTTGGAAACTCGAGTTAACTGCCAACTATCTGATGTTATCCAAAACTCAGTTCCCAGTGAACTCAACAAGTTGGTTCCATTTCAACTAGACGAAGTTGCTGATCTAGCCCTTGGTGTCCAACTAAATGTCTTTGACTGTGGTGGAGTTGCAATTGGTGCTTGCATTTCTCACAAGATTGCAGATGGTTTATCGTTTTTAATGTTCATCAAAACTTGGGCAGCCACTGCTAGAAAAGATCATTATCATCAGACAGATGATCATGCAGTTATGTCTCCAAAGTTTGTATCAGCCACACTGTTCCCACCAAGGAACATATCCGGGTATAATCCACGTGTTGGGATCACAAAAGAGAATATCGTAACAAAGAGCAACGTATGCAGAGACATCAGACTGAAGACCAAGAAACGCCCGTCTCGGGTTGTGGCATTGTCTGCCTTCTTGTGGAACAGGTTTATGGCCGAGGTTGCAAAAGAAGAAGAATCAAGTTCGAGAAGGTCGTACACGGTTGTGTATATTATGGATCTCCGTTCGAGGTTTGACTCACCTCTGCCGCAGAATTGTTTTGGGAACTATTACCGGGCAGCCACTGCAACTCCAACATTGGTCGATGGGGAGGAGAGAGGTGGCCTTGTGAGGCAGGTGATTGAGGAAATAGAGAAGATTGATAGTAATTATATGAGGAAATTTCAAGATGGCTATAAGGAACATTTGGATTTCATGAGAAGAAGGATGGAAAGGGCTGCAAAGGGAGAGCTTGTTACAGTGACGTTTTCGAGTCTGTGCAGGTTTCCATTGTATGAGGCTGATTTTGGTTGGGGGAAGCCAGCGTGGGTGAGCATGGCTTCCATGAGAATCAACAACCAAATAGTTTTCATGGACACCAAATTGGCTGATGGAATAGAGTCGTATTTTAGCTTCAAGGAGGAAGACATGGCCAAGTTTGAACTTGACTCAGAGTTCCTCGGGTTGATTTCTCCAATTGGTAATGTCAAGAAAAATCCATTTGCACGTCTTTAA

Protein Analysis

435

Amino Acids

49.42

Weight (kDa)

6.43

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 417 4.2e-74 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 919
Acc36I ACCTGC 2 cut(s) 919, 1065
AccB7I CCANNNNNTGG 2 cut(s) 236, 626
AciI CCGC 1 cut(s) 842
AclWI GGATC 2 cut(s) 638, 819
AcoI YGGCCR 2 cut(s) 747, 1224
AcsI RAATTY 2 cut(s) 160, 974
AcuI CTGAAG 1 cut(s) 704
AcvI CACGTG 1 cut(s) 623
AfaI GTAC 1 cut(s) 791
AfiI CCNNNNNNNGG 4 cut(s) 236, 626, 706, 732
AflIII ACRYGT 2 cut(s) 255, 622
AgsI TTSAA 4 cut(s) 374, 980, 1210, 1235
AjiI CACGTC 2 cut(s) 256, 1301
AjuI GAANNNNNNNTTGG 4 cut(s) 72, 104, 324, 356
AleI CACNNNNGTG 1 cut(s) 797
AluBI AGCT 3 cut(s) 34, 1045, 1206
AluI AGCT 3 cut(s) 34, 1045, 1206
Alw26I GTCTC 4 cut(s) 28, 567, 666, 708
AlwI GGATC 2 cut(s) 638, 819
AlwNI CAGNNNCTG 2 cut(s) 515, 878
Ama87I CYCGRG 3 cut(s) 300, 705, 1253
AoxI GGCC 3 cut(s) 747, 916, 1224
ApeKI GCWGC 3 cut(s) 509, 872, 1031
ApoI RAATTY 2 cut(s) 160, 974
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 2 cut(s) 610, 869
AsuHPI GGTGA 3 cut(s) 825, 943, 1135
AvaI CYCGRG 3 cut(s) 300, 705, 1253
AvaII GGWCC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 1226
BarI GAAGNNNNNNTAC 2 cut(s) 1190, 1222
BbrPI CACGTG 1 cut(s) 623
BbsI GAAGAC 2 cut(s) 692, 1224
BbvI GCAGC 3 cut(s) 521, 884, 1018
BccI CCATC 6 cut(s) 52, 467, 893, 977, 1015, 1178
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 2 cut(s) 610, 869
BcoDI GTCTC 4 cut(s) 28, 567, 666, 708
BfaI CTAG 3 cut(s) 377, 395, 519
BfuAI ACCTGC 2 cut(s) 919, 1065
BisI GCNGC 4 cut(s) 510, 842, 873, 1032
BlsI GCNGC 4 cut(s) 511, 843, 874, 1033
Bme1390I CCNGG 2 cut(s) 610, 869
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 3 cut(s) 300, 705, 1253
BmgBI CACGTC 2 cut(s) 256, 1301
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 2 cut(s) 610, 869
BmrI ACTGGG 2 cut(s) 104, 339
BmuI ACTGGG 2 cut(s) 104, 339
BpiI GAAGAC 2 cut(s) 692, 1224
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpmI CTGGAG 1 cut(s) 10
BpuMI CCSGG 2 cut(s) 610, 869
BsaAI YACGTR 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 531
BsaJI CCNNGG 5 cut(s) 147, 400, 596, 750, 1252
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 868, 898, 1032, 1062
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 626, 706, 732
Bse1I ACTGG 2 cut(s) 110, 345
Bse8I GATNNNNATC 1 cut(s) 531
BseDI CCNNGG 5 cut(s) 147, 400, 596, 750, 1252
BseGI GGATG 2 cut(s) 143, 1026
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 4 cut(s) 236, 626, 706, 732
BseMII CTCAG 2 cut(s) 351, 1257
BseNI ACTGG 2 cut(s) 110, 345
BseRI GAGGAG 1 cut(s) 920
BseXI GCAGC 3 cut(s) 521, 884, 1018
BsgI GTGCAG 1 cut(s) 1093
BshFI GGCC 3 cut(s) 749, 918, 1226
BsiHKCI CYCGRG 3 cut(s) 300, 705, 1253
BsiSI CCGG 2 cut(s) 609, 868
BslI CCNNNNNNNGG 4 cut(s) 236, 626, 706, 732
BsmAI GTCTC 4 cut(s) 28, 567, 666, 708
BsmBI CGTCTC 1 cut(s) 708
BsnI GGCC 3 cut(s) 749, 918, 1226
BsoBI CYCGRG 3 cut(s) 300, 705, 1253
Bsp143I GATC 5 cut(s) 391, 526, 547, 630, 811
BspACI CCGC 1 cut(s) 842
BspANI GGCC 3 cut(s) 749, 918, 1226
BspCNI CTCAG 2 cut(s) 350, 1256
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 1 cut(s) 366
BspMI ACCTGC 2 cut(s) 919, 1065
BspPI GGATC 2 cut(s) 638, 819
BsrI ACTGG 2 cut(s) 110, 345
BssECI CCNNGG 5 cut(s) 147, 400, 596, 750, 1252
BssMI GATC 5 cut(s) 391, 526, 547, 630, 811
BssT1I CCWWGG 2 cut(s) 400, 596
Bst4CI ACNGT 4 cut(s) 428, 588, 796, 1054
BstAPI GCANNNNNTGC 5 cut(s) 446, 515, 666, 722, 878
BstBAI YACGTR 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 451, 1116
BstDEI CTNAG 2 cut(s) 337, 1243
BstF5I GGATG 2 cut(s) 143, 1026
BstKTI GATC 5 cut(s) 394, 529, 550, 633, 814
BstMAI GTCTC 4 cut(s) 28, 567, 666, 708
BstMBI GATC 5 cut(s) 391, 526, 547, 630, 811
BstMWI GCNNNNNNNGC 8 cut(s) 242, 395, 446, 515, 666, 722, 755, 878
BstSCI CCNGG 2 cut(s) 608, 867
BstV1I GCAGC 3 cut(s) 521, 884, 1018
BstV2I GAAGAC 2 cut(s) 692, 1224
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 3 cut(s) 749, 918, 1226
BtrI CACGTC 2 cut(s) 256, 1301
BtsCI GGATG 2 cut(s) 143, 1026
BtsI GCAGTG 2 cut(s) 513, 876
BtsIMutI CAGTG 5 cut(s) 352, 513, 584, 876, 1059
BveI ACCTGC 2 cut(s) 919, 1065
Cac8I GCNNGC 2 cut(s) 451, 1116
CaiI CAGNNNCTG 2 cut(s) 515, 878
CciI TCATGA 1 cut(s) 1008
Cfr13I GGNCC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 790
CviAII CATG 6 cut(s) 551, 1009, 1129, 1138, 1165, 1222
CviQI GTAC 1 cut(s) 790
DdeI CTNAG 2 cut(s) 337, 1243
DpnI GATC 5 cut(s) 393, 528, 549, 632, 813
DpnII GATC 5 cut(s) 391, 526, 547, 630, 811
EaeI YGGCCR 2 cut(s) 747, 1224
Eco130I CCWWGG 2 cut(s) 400, 596
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 704
Eco72I CACGTG 1 cut(s) 623
Eco88I CYCGRG 3 cut(s) 300, 705, 1253
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 400, 596
ErhI CCWWGG 2 cut(s) 400, 596
Esp3I CGTCTC 1 cut(s) 708
FaeI CATG 6 cut(s) 554, 1012, 1132, 1141, 1168, 1225
FatI CATG 6 cut(s) 550, 1008, 1128, 1137, 1164, 1221
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 4 cut(s) 510, 842, 873, 1032
FokI GGATG 2 cut(s) 130, 1033
Fsp4HI GCNGC 4 cut(s) 510, 842, 873, 1032
FspBI CTAG 3 cut(s) 377, 395, 519
GluI GCNGC 4 cut(s) 510, 842, 873, 1032
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 3 cut(s) 749, 918, 1226
HapII CCGG 2 cut(s) 609, 868
Hin1II CATG 6 cut(s) 554, 1012, 1132, 1141, 1168, 1225
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 7 cut(s) 184, 770, 830, 1066, 1143, 1193, 1241
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 2 cut(s) 609, 868
HphI GGTGA 3 cut(s) 825, 943, 1135
Hpy166II GTNNAC 3 cut(s) 307, 350, 792
Hpy188I TCNGA 7 cut(s) 41, 148, 208, 322, 540, 680, 1246
Hpy188III TCNNGA 6 cut(s) 27, 92, 780, 980, 1009, 1282
Hpy8I GTNNAC 3 cut(s) 307, 350, 792
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 4 cut(s) 298, 736, 777, 1011
HpyCH4III ACNGT 4 cut(s) 428, 588, 796, 1054
HpyCH4IV ACGT 5 cut(s) 255, 622, 663, 1058, 1300
HpyF10VI GCNNNNNNNGC 8 cut(s) 242, 395, 446, 515, 666, 722, 755, 878
HpyF3I CTNAG 2 cut(s) 337, 1243
HpySE526I ACGT 5 cut(s) 255, 622, 663, 1058, 1300
Hsp92II CATG 6 cut(s) 554, 1012, 1132, 1141, 1168, 1225
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 391, 526, 547, 630, 811
Lsp1109I GCAGC 3 cut(s) 521, 884, 1018
MaeI CTAG 3 cut(s) 377, 395, 519
MaeII ACGT 5 cut(s) 255, 622, 663, 1058, 1300
MaeIII GTNAC 3 cut(s) 649, 1048, 1054
MalI GATC 5 cut(s) 393, 528, 549, 632, 813
MboI GATC 5 cut(s) 391, 526, 547, 630, 811
MboII GAAGA 7 cut(s) 39, 697, 776, 779, 961, 1026, 1229
MfeI CAATTG 2 cut(s) 441, 1269
MflI RGATCY 1 cut(s) 811
MlsI TGGCCA 1 cut(s) 1226
MluCI AATT 8 cut(s) 160, 221, 441, 847, 961, 974, 1175, 1269
MluNI TGGCCA 1 cut(s) 1226
MlyI GAGTC 4 cut(s) 824, 1075, 1202, 1235
MmeI TCCRAC 3 cut(s) 77, 433, 911
Mox20I TGGCCA 1 cut(s) 1226
MscI TGGCCA 1 cut(s) 1226
MseI TTAA 3 cut(s) 306, 488, 1306
MslI CAYNNNNRTG 1 cut(s) 797
Msp20I TGGCCA 1 cut(s) 1226
MspI CCGG 2 cut(s) 609, 868
MspR9I CCNGG 2 cut(s) 610, 869
MunI CAATTG 2 cut(s) 441, 1269
MwoI GCNNNNNNNGC 8 cut(s) 242, 395, 446, 515, 666, 722, 755, 878
NciI CCSGG 2 cut(s) 610, 869
NdeII GATC 5 cut(s) 391, 526, 547, 630, 811
NlaIII CATG 6 cut(s) 554, 1012, 1132, 1141, 1168, 1225
NlaIV GGNNCC 1 cut(s) 366
NmeAIII GCCGAG 1 cut(s) 775
NmuCI GTSAC 1 cut(s) 1054
OliI CACNNNNGTG 1 cut(s) 797
PaeR7I CTCGAG 1 cut(s) 300
PagI TCATGA 1 cut(s) 1008
PaqCI CACCTGC 1 cut(s) 919
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PfeI GAWTC 3 cut(s) 184, 770, 1143
PflMI CCANNNNNTGG 2 cut(s) 236, 626
PkrI GCNGC 4 cut(s) 511, 843, 874, 1033
PleI GAGTC 4 cut(s) 824, 1074, 1201, 1235
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 4 cut(s) 824, 1074, 1201, 1235
Ppu21I YACGTR 1 cut(s) 623
PspCI CACGTG 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 217
PspXI VCTCGAGB 1 cut(s) 300
PstNI CAGNNNCTG 2 cut(s) 515, 878
PsuI RGATCY 1 cut(s) 811
RsaI GTAC 1 cut(s) 791
RsaNI GTAC 1 cut(s) 790
RseI CAYNNNNRTG 1 cut(s) 797
SaqAI TTAA 3 cut(s) 306, 488, 1306
SatI GCNGC 4 cut(s) 510, 842, 873, 1032
Sau3AI GATC 5 cut(s) 391, 526, 547, 630, 811
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 4 cut(s) 824, 1075, 1202, 1235
ScrFI CCNGG 2 cut(s) 610, 869
Sfr274I CTCGAG 1 cut(s) 300
SinI GGWCC 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse9I AATT 8 cut(s) 160, 221, 441, 847, 961, 974, 1175, 1269
SsiI CCGC 1 cut(s) 842
SspMI CTAG 3 cut(s) 377, 395, 519
StyD4I CCNGG 2 cut(s) 608, 867
StyI CCWWGG 2 cut(s) 400, 596
TaaI ACNGT 4 cut(s) 428, 588, 796, 1054
TaiI ACGT 5 cut(s) 258, 625, 666, 1061, 1303
TaqI TCGA 7 cut(s) 165, 182, 301, 779, 821, 897, 1064
TasI AATT 8 cut(s) 160, 221, 441, 847, 961, 974, 1175, 1269
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 3 cut(s) 184, 770, 1143
Tru1I TTAA 3 cut(s) 306, 488, 1306
Tru9I TTAA 3 cut(s) 306, 488, 1306
TscAI CASTG 5 cut(s) 352, 520, 591, 883, 1059
TseFI GTSAC 1 cut(s) 1054
TseI GCWGC 3 cut(s) 509, 872, 1031
Tsp45I GTSAC 1 cut(s) 1054
TspDTI ATGAA 6 cut(s) 17, 129, 250, 484, 997, 1153
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 5 cut(s) 352, 520, 591, 883, 1059
Van91I CCANNNNNTGG 2 cut(s) 236, 626
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 2 cut(s) 160, 974
XhoI CTCGAG 1 cut(s) 300
XspI CTAG 3 cut(s) 377, 395, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.