pycom13g09520

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008131
Physical Location & Seq
Forward (+)
6389407 .. 6390771
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09520.1

Sequence Viewer

Length: 1365 bp
ATGAAGATTGAAGTTGAAGTGATCTCCAAGTCCGTAGAGATGATCAAACCATCTTCTCCAACCCCAAATCATCTTCACCATCACCAGCTCTGCTTTCTCGATCAATTAGCTCCCCATGCATATATCCCCTTTCTCTTCTTCTACGAATCCACCGGCACCAAAACCATCACTCAGATATCAAACATCCTCAAGACGTCTCTCTCCAAAATACTATCCCAATACTACCCTTTTGCCGGCCGAGTGAAACACAATCTCTTTGTTCACTGCAGCGACCAAGGCGTGCCATTCTTCGAATCCAAGGTCAAATCCCAACCCCTTTCTGATGTGATCGCCAGCCCTTTTGTCTCCGAACTCAACAAGTTGTTGCCCTACAGGCTAGACGAAGTAACTGAAGTACCCCTTGGTGTTCAACTCAATGTGTTTGACCGTGGAGGATTTGCTATTGGCTTGTGTGTCTCTCATAGGATTGCAGATGGGTTAACATCTTTTACATTTGTGAACAGATGGGCAGCTCAAGCTAATTGTGATGAACAAGGGAAGAGTAATATTATTGTGGGTCCGGATTTTTCTGCGGCATCAATCTTCCCACCAAAAAATATGGATGGGTATTTAGGGGTTTCGTTTGTCAACAAGAAAGCAATCGTAACAAAGAGGTTTGTGTTCGATGGCTCAAAGGTTGAGGCTTTAAGATCGAGATATGAAGAGAGCATGAAGTTTTCCAAAACCCAAAAACGCCCTTCAAAAGTTGAGGCCTTATCAGCTTTTTTATGGAACATCTTTCTGACATTAAGGAGAAAAACAGATTGTGTCGGTATTCCAAATCAATCACGCTCTCAAACATTATACACCGTTGTTTATATTATGGATCTGCGGTCGAGGTTTGACCCACCTTTGCCTCAGCATGCTTTTGGGAATTATTACAGGGCTGCCACCGCAACTCCAACTTTGGTCAATGGGGAGGGGAGCCATCGATTGGTGAGGCAGGTGATAGAGGAAATTGAGAACATTGACAATAATTATATGAGGAGATTTCAAGAGGGATATCGAGAGCATTTGGATTTCATGAGGAAAAGAATGGAGAGGTTTGCAAAAGGTGAGCTGGTGACATTAACTTTTTCGAGTGTGTGCAGGTTTCCTATTTACGATGCTGATTTTGGTTGGGGGAAGCCTGCATGGGTGAGCATGGCTGCCATGAATATTAATAATCAGATAGTTTTCATGGACACCAAAAATGGCGATGGTATCGAGTCGTATTTTAGCTTGACAGAGGAAGACATGGCCAAGTTTGAACTTCACAAAGAGTTCATCGCATTGCTTAAATCTCCAGTTGGTAATGTAAAGGAAAATTCACTTTCACGTCTTTAA

Protein Analysis

455

Amino Acids

51.67

Weight (kDa)

8.91

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 435 8.2e-74 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 973
AatII GACGTC 1 cut(s) 197
Acc36I ACCTGC 2 cut(s) 973, 1119
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 973
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 3 cut(s) 572, 871, 933
AclWI GGATC 1 cut(s) 873
AcoI YGGCCR 2 cut(s) 235, 1278
AcsI RAATTY 1 cut(s) 1345
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 233, 973
AgsI TTSAA 6 cut(s) 11, 17, 410, 741, 1034, 1289
AjiI CACGTC 1 cut(s) 1358
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 7 cut(s) 88, 110, 512, 518, 761, 1099, 1260
AluI AGCT 7 cut(s) 88, 110, 512, 518, 761, 1099, 1260
Alw26I GTCTC 3 cut(s) 201, 349, 460
AlwI GGATC 1 cut(s) 873
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 3 cut(s) 235, 750, 1278
ApeKI GCWGC 4 cut(s) 267, 509, 926, 1187
ApoI RAATTY 1 cut(s) 1345
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
AseI ATTAAT 1 cut(s) 1200
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 7 cut(s) 68, 74, 988, 997, 1106, 1114, 1189
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 557
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BalI TGGCCA 1 cut(s) 1280
BanI GGYRCC 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 1278
BbvCI CCTCAGC 1 cut(s) 897
BbvI GCAGC 4 cut(s) 279, 521, 913, 1174
BccI CCATC 9 cut(s) 58, 87, 173, 467, 498, 596, 659, 975, 1232
BclI TGATCA 1 cut(s) 42
BcoDI GTCTC 3 cut(s) 201, 349, 460
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 2 cut(s) 265, 370
BfuAI ACCTGC 2 cut(s) 973, 1119
BglI GCCNNNNNGGC 1 cut(s) 373
BisI GCNGC 5 cut(s) 268, 510, 573, 927, 1188
BlsI GCNGC 5 cut(s) 269, 511, 574, 928, 1189
Bme18I GGWCC 1 cut(s) 557
BmgBI CACGTC 1 cut(s) 1358
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 3 cut(s) 157, 558, 965
BmsI GCATC 2 cut(s) 584, 1135
BpiI GAAGAC 1 cut(s) 1278
BpmI CTGGAG 1 cut(s) 1308
Bpu10I CCTNAGC 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 291
BpuEI CTTGAG 2 cut(s) 173, 498
Bsa29I ATCGAT 1 cut(s) 970
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 4 cut(s) 274, 297, 400, 427
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 2 cut(s) 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 973
Bse118I RCCGGY 2 cut(s) 152, 233
Bse1I ACTGG 1 cut(s) 1325
Bse3DI GCAATG 1 cut(s) 1310
BseAI TCCGGA 1 cut(s) 559
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 4 cut(s) 274, 297, 400, 427
BseGI GGATG 2 cut(s) 183, 607
BseLI CCNNNNNNNGG 2 cut(s) 233, 973
BseMI GCAATG 1 cut(s) 1310
BseMII CTCAG 2 cut(s) 185, 911
BseNI ACTGG 1 cut(s) 1325
BseRI GAGGAG 1 cut(s) 1039
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 4 cut(s) 279, 521, 913, 1174
BsgI GTGCAG 1 cut(s) 1147
Bsh1285I CGRYCG 2 cut(s) 238, 875
BshFI GGCC 3 cut(s) 237, 752, 1280
BshNI GGYRCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 970
BsiEI CGRYCG 2 cut(s) 238, 875
BsiSI CCGG 3 cut(s) 153, 234, 560
BslI CCNNNNNNNGG 2 cut(s) 233, 973
BsmAI GTCTC 3 cut(s) 201, 349, 460
BsmBI CGTCTC 1 cut(s) 201
BsnI GGCC 3 cut(s) 237, 752, 1280
Bsp119I TTCGAA 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 559
Bsp143I GATC 6 cut(s) 21, 42, 100, 327, 689, 865
BspACI CCGC 3 cut(s) 572, 871, 933
BspANI GGCC 3 cut(s) 237, 752, 1280
BspCNI CTCAG 2 cut(s) 184, 910
BspDI ATCGAT 1 cut(s) 970
BspEI TCCGGA 1 cut(s) 559
BspHI TCATGA 1 cut(s) 1062
BspLI GGNNCC 3 cut(s) 157, 558, 965
BspMAI CTGCAG 1 cut(s) 269
BspMI ACCTGC 2 cut(s) 973, 1119
BspPI GGATC 1 cut(s) 873
BspT104I TTCGAA 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 1310
BsrFI RCCGGY 2 cut(s) 152, 233
BsrI ACTGG 1 cut(s) 1325
BssAI RCCGGY 2 cut(s) 152, 233
BssECI CCNNGG 4 cut(s) 274, 297, 400, 427
BssMI GATC 6 cut(s) 21, 42, 100, 327, 689, 865
BssNI GRCGYC 1 cut(s) 194
BssT1I CCWWGG 3 cut(s) 274, 297, 400
Bst4CI ACNGT 2 cut(s) 428, 850
Bst6I CTCTTC 3 cut(s) 140, 533, 696
BstACI GRCGYC 1 cut(s) 194
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 5 cut(s) 235, 281, 334, 903, 1170
BstDEI CTNAG 2 cut(s) 171, 897
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 2 cut(s) 183, 607
BstKTI GATC 6 cut(s) 24, 45, 103, 330, 692, 868
BstMAI GTCTC 3 cut(s) 201, 349, 460
BstMBI GATC 6 cut(s) 21, 42, 100, 327, 689, 865
BstMCI CGRYCG 2 cut(s) 238, 875
BstMWI GCNNNNNNNGC 6 cut(s) 116, 276, 373, 515, 758, 932
BstNSI RCATGY 1 cut(s) 905
BstSFI CTRYAG 2 cut(s) 265, 370
BstV1I GCAGC 4 cut(s) 279, 521, 913, 1174
BstV2I GAAGAC 1 cut(s) 1278
BstX2I RGATCY 1 cut(s) 865
BstYI RGATCY 1 cut(s) 865
BstZI CGGCCG 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 970
BsuRI GGCC 3 cut(s) 237, 752, 1280
BsuTUI ATCGAT 1 cut(s) 970
BtgI CCRYGG 1 cut(s) 427
BtgZI GCGATG 2 cut(s) 1251, 1291
BtrI CACGTC 1 cut(s) 1358
BtsCI GGATG 2 cut(s) 183, 607
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 262
BveI ACCTGC 2 cut(s) 973, 1119
Cac8I GCNNGC 5 cut(s) 235, 281, 334, 903, 1170
CciI TCATGA 1 cut(s) 1062
Cfr10I RCCGGY 2 cut(s) 152, 233
Cfr13I GGNCC 1 cut(s) 557
ClaI ATCGAT 1 cut(s) 970
Csp6I GTAC 1 cut(s) 395
CviAII CATG 9 cut(s) 116, 709, 902, 1063, 1173, 1183, 1192, 1219, 1276
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 2 cut(s) 171, 897
DpnI GATC 6 cut(s) 23, 44, 102, 329, 691, 867
DpnII GATC 6 cut(s) 21, 42, 100, 327, 689, 865
EaeI YGGCCR 2 cut(s) 235, 1278
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 140, 533, 696
EarI CTCTTC 3 cut(s) 140, 533, 696
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 274, 297, 400
Eco147I AGGCCT 1 cut(s) 752
Eco32I GATATC 2 cut(s) 177, 1043
Eco47I GGWCC 1 cut(s) 557
Eco52I CGGCCG 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 411
EcoRV GATATC 2 cut(s) 177, 1043
EcoT14I CCWWGG 3 cut(s) 274, 297, 400
EcoT22I ATGCAT 1 cut(s) 121
ErhI CCWWGG 3 cut(s) 274, 297, 400
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 9 cut(s) 119, 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 9 cut(s) 115, 708, 901, 1062, 1172, 1182, 1191, 1218, 1275
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 5 cut(s) 268, 510, 573, 927, 1188
FokI GGATG 2 cut(s) 170, 614
Fsp4HI GCNGC 5 cut(s) 268, 510, 573, 927, 1188
FspBI CTAG 1 cut(s) 377
GluI GCNGC 5 cut(s) 268, 510, 573, 927, 1188
GsuI CTGGAG 1 cut(s) 1308
HaeIII GGCC 3 cut(s) 237, 752, 1280
HapII CCGG 3 cut(s) 153, 234, 560
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 9 cut(s) 119, 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
HincII GTYRAC 2 cut(s) 480, 628
HindII GTYRAC 2 cut(s) 480, 628
HinfI GANTC 3 cut(s) 146, 293, 1247
HpaI GTTAAC 1 cut(s) 480
HpaII CCGG 3 cut(s) 153, 234, 560
HphI GGTGA 7 cut(s) 68, 74, 988, 997, 1106, 1114, 1189
Hpy166II GTNNAC 4 cut(s) 262, 480, 499, 628
Hpy188I TCNGA 5 cut(s) 174, 322, 349, 783, 1209
Hpy188III TCNNGA 7 cut(s) 98, 190, 560, 693, 1034, 1046, 1063
Hpy8I GTNNAC 4 cut(s) 262, 480, 499, 628
HpyAV CCTTC 1 cut(s) 747
HpyCH4III ACNGT 2 cut(s) 428, 850
HpyCH4IV ACGT 2 cut(s) 194, 1357
HpyCH4V TGCA 6 cut(s) 119, 267, 470, 1088, 1128, 1172
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 276, 373, 515, 758, 932
HpyF3I CTNAG 2 cut(s) 171, 897
HpySE526I ACGT 2 cut(s) 194, 1357
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 9 cut(s) 119, 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
Kpn2I TCCGGA 1 cut(s) 559
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 480
Kzo9I GATC 6 cut(s) 21, 42, 100, 327, 689, 865
LmnI GCTCC 2 cut(s) 115, 963
Lsp1109I GCAGC 4 cut(s) 279, 521, 913, 1174
LweI GCATC 2 cut(s) 584, 1135
MaeI CTAG 1 cut(s) 377
MaeII ACGT 2 cut(s) 194, 1357
MaeIII GTNAC 3 cut(s) 385, 643, 1102
MalI GATC 6 cut(s) 23, 44, 102, 329, 691, 867
MboI GATC 6 cut(s) 21, 42, 100, 327, 689, 865
MflI RGATCY 1 cut(s) 865
MlsI TGGCCA 1 cut(s) 1280
MluCI AATT 6 cut(s) 104, 520, 913, 996, 1015, 1345
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 1 cut(s) 1256
MmeI TCCRAC 2 cut(s) 83, 965
Mox20I TGGCCA 1 cut(s) 1280
Mph1103I ATGCAT 1 cut(s) 121
MroI TCCGGA 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 233
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 7 cut(s) 479, 686, 788, 1109, 1200, 1317, 1363
Msp20I TGGCCA 1 cut(s) 1280
MspI CCGG 3 cut(s) 153, 234, 560
MwoI GCNNNNNNNGC 6 cut(s) 116, 276, 373, 515, 758, 932
NaeI GCCGGC 1 cut(s) 235
NdeII GATC 6 cut(s) 21, 42, 100, 327, 689, 865
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 9 cut(s) 119, 712, 905, 1066, 1176, 1186, 1195, 1222, 1279
NlaIV GGNNCC 3 cut(s) 157, 558, 965
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 1102
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 905
NspV TTCGAA 1 cut(s) 291
PaeI GCATGC 1 cut(s) 905
PagI TCATGA 1 cut(s) 1062
PaqCI CACCTGC 1 cut(s) 973
PceI AGGCCT 1 cut(s) 752
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 146, 293
PflMI CCANNNNNTGG 1 cut(s) 973
PkrI GCNGC 5 cut(s) 269, 511, 574, 928, 1189
PleI GAGTC 1 cut(s) 1255
PpsI GAGTC 1 cut(s) 1255
PshBI ATTAAT 1 cut(s) 1200
PspN4I GGNNCC 3 cut(s) 157, 558, 965
PspPI GGNCC 1 cut(s) 557
PstI CTGCAG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 865
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
SaqAI TTAA 7 cut(s) 479, 686, 788, 1109, 1200, 1317, 1363
SatI GCNGC 5 cut(s) 268, 510, 573, 927, 1188
Sau3AI GATC 6 cut(s) 21, 42, 100, 327, 689, 865
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 1 cut(s) 1256
SfaNI GCATC 2 cut(s) 584, 1135
SfcI CTRYAG 2 cut(s) 265, 370
SfuI TTCGAA 1 cut(s) 291
SinI GGWCC 1 cut(s) 557
SmlI CTYRAG 2 cut(s) 188, 513
SmoI CTYRAG 2 cut(s) 188, 513
SphI GCATGC 1 cut(s) 905
Sse9I AATT 6 cut(s) 104, 520, 913, 996, 1015, 1345
SseBI AGGCCT 1 cut(s) 752
SsiI CCGC 3 cut(s) 572, 871, 933
SspI AATATT 2 cut(s) 547, 1198
SspMI CTAG 1 cut(s) 377
StuI AGGCCT 1 cut(s) 752
StyI CCWWGG 3 cut(s) 274, 297, 400
TaaI ACNGT 2 cut(s) 428, 850
TaiI ACGT 2 cut(s) 197, 1360
TaqI TCGA 9 cut(s) 99, 291, 663, 692, 875, 970, 1045, 1118, 1245
TasI AATT 6 cut(s) 104, 520, 913, 996, 1015, 1345
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 2 cut(s) 146, 293
Tru1I TTAA 7 cut(s) 479, 686, 788, 1109, 1200, 1317, 1363
Tru9I TTAA 7 cut(s) 479, 686, 788, 1109, 1200, 1317, 1363
TscAI CASTG 1 cut(s) 269
TseFI GTSAC 1 cut(s) 1102
TseI GCWGC 4 cut(s) 267, 509, 926, 1187
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 8 cut(s) 17, 543, 714, 725, 1051, 1207, 1208, 1294
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 269
Van91I CCANNNNNTGG 1 cut(s) 973
VpaK11BI GGWCC 1 cut(s) 557
VspI ATTAAT 1 cut(s) 1200
XapI RAATTY 1 cut(s) 1345
XceI RCATGY 1 cut(s) 905
XspI CTAG 1 cut(s) 377
ZraI GACGTC 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.