Rh4AG308900

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008131
Physical Location & Seq
Reverse (-)
62058168 .. 62059487
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG308900.1

Sequence Viewer

Length: 1320 bp
ATGAAGATTGAAGTTGAAGTAATCTCTCAAGAAACAATAAAACCTTCAACTCCAACCCCTCACCATCTCCGCCATCACCAGCTCTCCTTTCTCGACCAGTTAGCTCCCCACGCCTACGACCCTTTCCTCTACTTCTACTCCCTCCCCAATACTCACAACAACCACACCGAAATCTCCACCCACCTCAAGAAATCTCTCTCCAAAATCCTAACCCAGTACTACCCAATAGCCGGCCGAGTCAAATTCAACCTCTTTGTGGATTGCAACGACCATGGAATCCCCTTCTACGAAGCTCGAGTCAAATCCCACTCACTCTCTGATGTCATCACCAATCCCTCCCTCCCCGAATTGAACAACTTGTTGCCTTTCGAACTCGACGCGGTCACAGACATAGCTCTCGGAGTTCAACTGAATCTGTTTCCGTGCGGAGGAATTGCTATTGGGTTGTGCGCTTCGCATCGAATTGCAGATGGGTTGTCATTTTTCACGTTTGTCAAGAACTGGGCATCAACTTCTTGTGGAAATGAGTGTGTGAATCCCGATTTTTCTGCAGCTTCGATCTTCCCTCCAAAGAATATGGATGGGTATAACGGCGTTCCTCTCCCGAAAAGGAAAGATATTGTGACCAAGAGGTTTGTGTTTGATGGGTCGAAGATCGAAGCTTTACGAGCAACGTATGCAGAGAGCATCAGACTGAAGACCAAGAAACGCCCGTCGCGGGTTGAGGCATTGTCTGCCTTCTTGTGGAACAGGTTTATGGCCGAGGTTGCAAAAGAAGAAGAATCAAGTTCAAGAAGGTTGTACACGGTTGTGTATATTATGGATCTCCGTTCGAGGTTTGACTCACCTCTGCCGCAGAATAGTTTTGGGAACTATTACCGGGCAGCCACTGCAACTCCAACATTGGTTGATGGGGAGGAGAGAGGTGGCCTTGTGAGGCAGGTGACTGAGGAAATAGAGAAGATTGATAGTAAGTATATGAGGAAATTTCAAGATGGCTTTAAGGAACATTTGGATTTCATGAGGAGAAGGATGGAAAGGGCTGCAAAAGGAGAGCTTGTTACAGTGACGTTTTCAAGTCTGTGCAGGTTTCCATTGTATGCGGCTGATTTTGGTTGGGGGAAGCCAGCGTGGGTGAGCATGGCTTCCATGAGAATCAACAACCAAATAGTTTTCATGGACACCAAATTGGCTGATGGAATAGAGTCGTATTTTAGCTTCAAGGAGGAAGACATGGCCAAGTTTGAACTTGACTCAGAGTTCCTCGAGTTGATTTCTCCAATTGGTAATGTCAAGAAAAATCCATTTGCACGTCTTTAA

Protein Analysis

439

Amino Acids

49.98

Weight (kDa)

8.09

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 421 1.3e-80 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 931
Acc36I ACCTGC 2 cut(s) 931, 1077
AccII CGCG 2 cut(s) 380, 718
AciI CCGC 6 cut(s) 70, 380, 426, 718, 854, 1103
AclWI GGATC 1 cut(s) 831
AcoI YGGCCR 3 cut(s) 232, 759, 1236
AcsI RAATTY 2 cut(s) 242, 986
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 2 cut(s) 218, 803
AfiI CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
AjiI CACGTC 1 cut(s) 1313
AleI CACNNNNGTG 1 cut(s) 809
AluBI AGCT 8 cut(s) 82, 104, 293, 395, 554, 662, 1057, 1218
AluI AGCT 8 cut(s) 82, 104, 293, 395, 554, 662, 1057, 1218
AlwI GGATC 1 cut(s) 831
AlwNI CAGNNNCTG 1 cut(s) 890
Ama87I CYCGRG 2 cut(s) 294, 1265
AoxI GGCC 4 cut(s) 232, 759, 928, 1236
ApeKI GCWGC 3 cut(s) 551, 884, 1043
ApoI RAATTY 2 cut(s) 242, 986
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspLEI GCGC 1 cut(s) 452
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 6 cut(s) 53, 68, 319, 837, 955, 1147
AsuII TTCGAA 1 cut(s) 369
AvaI CYCGRG 2 cut(s) 294, 1265
BalI TGGCCA 1 cut(s) 1238
BarI GAAGNNNNNNTAC 2 cut(s) 1202, 1234
BbsI GAAGAC 2 cut(s) 704, 1236
BbvI GCAGC 3 cut(s) 563, 896, 1030
BccI CCATC 9 cut(s) 72, 81, 464, 575, 638, 905, 989, 1027, 1190
BceAI ACGGC 1 cut(s) 607
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BcnI CCSGG 1 cut(s) 881
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 2 cut(s) 931, 1077
BisI GCNGC 5 cut(s) 552, 854, 885, 1044, 1104
BlsI GCNGC 5 cut(s) 553, 855, 886, 1045, 1105
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 881
BmeT110I CYCGRG 2 cut(s) 294, 1265
BmgBI CACGTC 1 cut(s) 1313
BmrFI CCNGG 1 cut(s) 881
BmrI ACTGGG 2 cut(s) 208, 511
BmsI GCATC 3 cut(s) 466, 515, 696
BmuI ACTGGG 2 cut(s) 208, 511
BpiI GAAGAC 2 cut(s) 704, 1236
Bpu14I TTCGAA 1 cut(s) 369
BpuEI CTTGAG 2 cut(s) 12, 170
BpuMI CCSGG 1 cut(s) 881
BsaJI CCNNGG 2 cut(s) 271, 762
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 122, 152, 880, 910, 1044, 1074
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 3 cut(s) 97, 214, 506
BseDI CCNNGG 2 cut(s) 271, 762
BseGI GGATG 2 cut(s) 586, 1038
BseLI CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
BseMII CTCAG 2 cut(s) 939, 1269
BseNI ACTGG 3 cut(s) 97, 214, 506
BseRI GAGGAG 2 cut(s) 932, 1039
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 3 cut(s) 563, 896, 1030
BsgI GTGCAG 1 cut(s) 1105
Bsh1236I CGCG 2 cut(s) 380, 718
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 4 cut(s) 234, 761, 930, 1238
BsiEI CGRYCG 1 cut(s) 235
BsiHKCI CYCGRG 2 cut(s) 294, 1265
BsiSI CCGG 2 cut(s) 231, 880
BslI CCNNNNNNNGG 5 cut(s) 230, 256, 428, 718, 744
BsnI GGCC 4 cut(s) 234, 761, 930, 1238
BsoBI CYCGRG 2 cut(s) 294, 1265
Bsp119I TTCGAA 1 cut(s) 369
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 3 cut(s) 558, 654, 823
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 6 cut(s) 70, 380, 426, 718, 854, 1103
BspANI GGCC 4 cut(s) 234, 761, 930, 1238
BspCNI CTCAG 2 cut(s) 940, 1268
BspFNI CGCG 2 cut(s) 380, 718
BspHI TCATGA 1 cut(s) 1020
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 2 cut(s) 931, 1077
BspPI GGATC 1 cut(s) 831
BspT104I TTCGAA 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 3 cut(s) 97, 214, 506
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 2 cut(s) 271, 762
BssMI GATC 3 cut(s) 558, 654, 823
BssT1I CCWWGG 1 cut(s) 271
Bst4CI ACNGT 2 cut(s) 808, 1066
BstAPI GCANNNNNTGC 3 cut(s) 677, 734, 890
BstAUI TGTACA 1 cut(s) 801
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 232, 1128
BstDEI CTNAG 2 cut(s) 948, 1255
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 586, 1038
BstFNI CGCG 2 cut(s) 380, 718
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 3 cut(s) 561, 657, 826
BstMBI GATC 3 cut(s) 558, 654, 823
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 6 cut(s) 110, 668, 677, 734, 767, 890
BstSCI CCNGG 1 cut(s) 879
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 2 cut(s) 380, 718
BstV1I GCAGC 3 cut(s) 563, 896, 1030
BstV2I GAAGAC 2 cut(s) 704, 1236
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 4 cut(s) 234, 761, 930, 1238
BtgI CCRYGG 1 cut(s) 271
BtrI CACGTC 1 cut(s) 1313
BtsCI GGATG 2 cut(s) 586, 1038
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 2 cut(s) 888, 1071
BveI ACCTGC 2 cut(s) 931, 1077
Cac8I GCNNGC 2 cut(s) 232, 1128
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 1 cut(s) 452
Cfr10I RCCGGY 1 cut(s) 230
CseI GACGC 1 cut(s) 386
Csp6I GTAC 2 cut(s) 217, 802
CviAII CATG 6 cut(s) 272, 1021, 1141, 1150, 1177, 1234
CviQI GTAC 2 cut(s) 217, 802
DdeI CTNAG 2 cut(s) 948, 1255
DpnI GATC 3 cut(s) 560, 656, 825
DpnII GATC 3 cut(s) 558, 654, 823
EaeI YGGCCR 3 cut(s) 232, 759, 1236
EagI CGGCCG 1 cut(s) 232
EciI GGCGGA 1 cut(s) 59
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 271
Eco52I CGGCCG 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 716
Eco88I CYCGRG 2 cut(s) 294, 1265
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 6 cut(s) 275, 1024, 1144, 1153, 1180, 1237
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FatI CATG 6 cut(s) 271, 1020, 1140, 1149, 1176, 1233
FauI CCCGC 1 cut(s) 711
Fnu4HI GCNGC 5 cut(s) 552, 854, 885, 1044, 1104
FokI GGATG 2 cut(s) 593, 1045
Fsp4HI GCNGC 5 cut(s) 552, 854, 885, 1044, 1104
GlaI GCGC 1 cut(s) 451
GluI GCNGC 5 cut(s) 552, 854, 885, 1044, 1104
HaeIII GGCC 4 cut(s) 234, 761, 930, 1238
HapII CCGG 2 cut(s) 231, 880
HgaI GACGC 1 cut(s) 386
HhaI GCGC 1 cut(s) 452
Hin1II CATG 6 cut(s) 275, 1024, 1144, 1153, 1180, 1237
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HindIII AAGCTT 1 cut(s) 660
HpaII CCGG 2 cut(s) 231, 880
HphI GGTGA 6 cut(s) 53, 68, 319, 837, 955, 1147
Hpy166II GTNNAC 1 cut(s) 804
Hpy188I TCNGA 4 cut(s) 319, 401, 692, 1258
Hpy8I GTNNAC 1 cut(s) 804
Hpy99I CGWCG 2 cut(s) 380, 718
HpyAV CCTTC 5 cut(s) 54, 292, 748, 789, 1023
HpyCH4III ACNGT 2 cut(s) 808, 1066
HpyCH4IV ACGT 4 cut(s) 488, 674, 1070, 1312
HpyCH4V TGCA 9 cut(s) 264, 467, 551, 680, 770, 893, 1046, 1086, 1310
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 668, 677, 734, 767, 890
HpyF3I CTNAG 2 cut(s) 948, 1255
HpySE526I ACGT 4 cut(s) 488, 674, 1070, 1312
Hsp92II CATG 6 cut(s) 275, 1024, 1144, 1153, 1180, 1237
HspAI GCGC 1 cut(s) 450
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 3 cut(s) 558, 654, 823
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 3 cut(s) 563, 896, 1030
LweI GCATC 3 cut(s) 466, 515, 696
MaeII ACGT 4 cut(s) 488, 674, 1070, 1312
MaeIII GTNAC 5 cut(s) 382, 622, 943, 1060, 1066
MalI GATC 3 cut(s) 560, 656, 825
MboI GATC 3 cut(s) 558, 654, 823
MboII GAAGA 8 cut(s) 16, 553, 664, 709, 788, 791, 973, 1241
MfeI CAATTG 1 cut(s) 1281
MflI RGATCY 1 cut(s) 823
MlsI TGGCCA 1 cut(s) 1238
MluCI AATT 7 cut(s) 242, 347, 432, 462, 986, 1187, 1281
MluNI TGGCCA 1 cut(s) 1238
MlyI GAGTC 5 cut(s) 246, 306, 836, 1214, 1247
MmeI TCCRAC 2 cut(s) 77, 923
Mox20I TGGCCA 1 cut(s) 1238
MroNI GCCGGC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 1238
MseI TTAA 2 cut(s) 1002, 1318
MslI CAYNNNNRTG 1 cut(s) 809
Msp20I TGGCCA 1 cut(s) 1238
MspI CCGG 2 cut(s) 231, 880
MspR9I CCNGG 1 cut(s) 881
MunI CAATTG 1 cut(s) 1281
MvnI CGCG 2 cut(s) 380, 718
MwoI GCNNNNNNNGC 6 cut(s) 110, 668, 677, 734, 767, 890
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 881
NcoI CCATGG 1 cut(s) 271
NdeII GATC 3 cut(s) 558, 654, 823
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 6 cut(s) 275, 1024, 1144, 1153, 1180, 1237
NmeAIII GCCGAG 2 cut(s) 260, 787
NmuCI GTSAC 4 cut(s) 382, 622, 943, 1066
NspV TTCGAA 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 809
PaeR7I CTCGAG 2 cut(s) 294, 1265
PagI TCATGA 1 cut(s) 1020
PaqCI CACCTGC 1 cut(s) 931
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 5 cut(s) 276, 412, 535, 782, 1155
PflFI GACNNNGTC 1 cut(s) 380
PkrI GCNGC 5 cut(s) 553, 855, 886, 1045, 1105
PleI GAGTC 5 cut(s) 245, 305, 836, 1213, 1247
PpsI GAGTC 5 cut(s) 245, 305, 836, 1213, 1247
PspXI VCTCGAGB 2 cut(s) 294, 1265
PstI CTGCAG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 1 cut(s) 823
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 2 cut(s) 218, 803
RsaNI GTAC 2 cut(s) 217, 802
RseI CAYNNNNRTG 1 cut(s) 809
SaqAI TTAA 2 cut(s) 1002, 1318
SatI GCNGC 5 cut(s) 552, 854, 885, 1044, 1104
Sau3AI GATC 3 cut(s) 558, 654, 823
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 5 cut(s) 246, 306, 836, 1214, 1247
ScrFI CCNGG 1 cut(s) 881
SfaNI GCATC 3 cut(s) 466, 515, 696
SfcI CTRYAG 1 cut(s) 549
Sfr274I CTCGAG 2 cut(s) 294, 1265
SfuI TTCGAA 1 cut(s) 369
SlaI CTCGAG 2 cut(s) 294, 1265
SmiMI CAYNNNNRTG 1 cut(s) 809
SmlI CTYRAG 4 cut(s) 27, 185, 294, 1265
SmoI CTYRAG 4 cut(s) 27, 185, 294, 1265
Sse9I AATT 7 cut(s) 242, 347, 432, 462, 986, 1187, 1281
SsiI CCGC 6 cut(s) 70, 380, 426, 718, 854, 1103
StyD4I CCNGG 1 cut(s) 879
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 808, 1066
TaiI ACGT 4 cut(s) 491, 677, 1073, 1315
TasI AATT 7 cut(s) 242, 347, 432, 462, 986, 1187, 1281
TatI WGTACW 2 cut(s) 216, 801
TauI GCSGC 2 cut(s) 856, 1106
TfiI GAWTC 5 cut(s) 276, 412, 535, 782, 1155
Tru1I TTAA 2 cut(s) 1002, 1318
Tru9I TTAA 2 cut(s) 1002, 1318
TscAI CASTG 2 cut(s) 895, 1071
TseFI GTSAC 4 cut(s) 382, 622, 943, 1066
TseI GCWGC 3 cut(s) 551, 884, 1043
Tsp45I GTSAC 4 cut(s) 382, 622, 943, 1066
TspDTI ATGAA 3 cut(s) 17, 1009, 1165
TspGWI ACGGA 2 cut(s) 411, 818
TspRI CASTG 2 cut(s) 895, 1071
Tth111I GACNNNGTC 1 cut(s) 380
XapI RAATTY 2 cut(s) 242, 986
XhoI CTCGAG 2 cut(s) 294, 1265
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.