Rw0G003010

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00098
OG0008131
Physical Location & Seq
Forward (+)
31184 .. 43495
12312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003010.1

Sequence Viewer

Length: 1353 bp
ATGATCATGAAGGTTGAGGTTGAGGTTGTCACCAGAGATCTTATCAGACCATCTTCTCCAACCCCTGACCACCTCCACAGATATCAACTCTCCTTTCTTGACCAGATATCTCCCCCAGTCTTTAGTCCTTTGGTTCTATTTTATTCATCATCCGAGGCTGATACAAATTTTGACATTGCTGATATATCGAATCGGCTAAAGAAATCCCTCTCCGATGTCTTGGTCCAATTCTACCCACTTGCTGGACGAGCAAATGACAACATGTTCATAGACTGCAACGACAAGGGCATACCCTTCTTGGAAACTCGAGTTAACTGCCAACTATCTGATGTTATCCAAAACCCAGTTCCCAGTGAACTCAACAAGTTGGTTCCATTTCAACTAGACGAAGTTGCTGATCTAGCCCTTGGTGTCCAACTAAATGTCTTTGACTGTGGTGGAGTTGCAATTGGTGCTTGCATTTCTCACAAGATTGCAGATGGTTTATCGTTTTTAATGTTCATCAAAACTTGGGCAGCCACTGCTAGAAAAGATCATTATCATCAGACAGATGATCATGCAGTTATGTCTCCAAAGTTTGTATCAGCCACACTGTTCCCACCAAGGAACATATCCGGGTATAATCCACGTGTTGGGATCACAAAAGAGAATATCGTAACAAAGAGGTTTGTGTTTGATGGGTCGAAGATCGAAGCTTTACGAGCAACGTATGCAGAGAGCATCAGACTGAAGACCAAGAAACGCCCGTCTCGGGTTGAGGCGTTGTCTGCCTTCTTGTGGAACAGGTTTATGGCCGAGGTTGCAAAAGAAGAAGAATCAAGTTCAAGAAGGTTGTACACGGTTGTGTATATTATGGATCTCCGTTCGAGGTTTGACTCACCTCTGCCGCAGAATAGTTTTGGGAACTATTACCGGGCAGCCACTGCAACTCCAACATTGATTAATGGGGAGGAGAGAGGTGTCCTTGTGAGGCAGGTGATTGAGGAAATAGAGAAGATTGATAGTAAGTATATGAGGAAATTTCAAGATGGCTATAAGGAACATTTGGATTTCATGAGGAGAAGGATGGAAAGGGCTGCAAAAGGAGAGCTTGTTACAGTGACGTTTTCGAGTCTCTGCAGGTTTCCATTGTATGAGGCTGATTTTGGTTGGGGGAAGCCAGCGTGGGTGAGCATGGCTTCCATGAGAATCAACAACCAAATAGTTTTCATGGACACCAAATTGGCTGATGGAATAGAGTCGTATTTTAGCTTCAAGGAGGAAGACATGGCCAAGTTTGAACTTGACTCAGAGTTCCTCCGGTTGATTTCTCCAATTGGTAATGTCAAGAAAAATCCATTTGCACGTCTTTAA

Protein Analysis

450

Amino Acids

51.32

Weight (kDa)

7.62

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 432 1e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc36I ACCTGC 2 cut(s) 964, 1110
AccB7I CCANNNNNTGG 2 cut(s) 242, 632
AciI CCGC 1 cut(s) 887
AclWI GGATC 2 cut(s) 644, 864
AcoI YGGCCR 2 cut(s) 792, 1269
AcsI RAATTY 2 cut(s) 166, 1019
AcuI CTGAAG 1 cut(s) 749
AcvI CACGTG 1 cut(s) 629
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 4 cut(s) 242, 632, 751, 777
AflIII ACRYGT 2 cut(s) 261, 628
AgsI TTSAA 5 cut(s) 380, 825, 1025, 1255, 1280
AjiI CACGTC 1 cut(s) 1346
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 3 cut(s) 695, 1090, 1251
AluI AGCT 3 cut(s) 695, 1090, 1251
Alw26I GTCTC 3 cut(s) 573, 753, 1118
AlwI GGATC 2 cut(s) 644, 864
AlwNI CAGNNNCTG 2 cut(s) 521, 923
Ama87I CYCGRG 2 cut(s) 306, 750
AoxI GGCC 2 cut(s) 792, 1269
ApeKI GCWGC 3 cut(s) 515, 917, 1076
ApoI RAATTY 2 cut(s) 166, 1019
AseI ATTAAT 1 cut(s) 942
AspS9I GGNCC 1 cut(s) 223
AsuC2I CCSGG 2 cut(s) 616, 914
AsuHPI GGTGA 4 cut(s) 22, 870, 988, 1180
AvaI CYCGRG 2 cut(s) 306, 750
AvaII GGWCC 1 cut(s) 223
BalI TGGCCA 1 cut(s) 1271
BarI GAAGNNNNNNTAC 2 cut(s) 1235, 1267
BbrPI CACGTG 1 cut(s) 629
BbsI GAAGAC 2 cut(s) 737, 1269
BbvI GCAGC 3 cut(s) 527, 929, 1063
BccI CCATC 6 cut(s) 58, 473, 671, 1022, 1060, 1223
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BclI TGATCA 2 cut(s) 3, 553
BcnI CCSGG 2 cut(s) 616, 914
BcoDI GTCTC 3 cut(s) 573, 753, 1118
BfaI CTAG 3 cut(s) 383, 401, 525
BfmI CTRYAG 1 cut(s) 1117
BfuAI ACCTGC 2 cut(s) 964, 1110
BglII AGATCT 1 cut(s) 37
BisI GCNGC 4 cut(s) 516, 887, 918, 1077
BlsI GCNGC 4 cut(s) 517, 888, 919, 1078
Bme1390I CCNGG 2 cut(s) 616, 914
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 2 cut(s) 306, 750
BmgBI CACGTC 1 cut(s) 1346
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 2 cut(s) 616, 914
BmrI ACTGGG 3 cut(s) 110, 338, 345
BmsI GCATC 1 cut(s) 729
BmuI ACTGGG 3 cut(s) 110, 338, 345
BpiI GAAGAC 2 cut(s) 737, 1269
BpuMI CCSGG 2 cut(s) 616, 914
BsaAI YACGTR 1 cut(s) 629
BsaBI GATNNNNATC 1 cut(s) 537
BsaJI CCNNGG 4 cut(s) 153, 406, 602, 795
BsaWI WCCGGW 1 cut(s) 1299
BsaXI ACNNNNNCTCC 4 cut(s) 913, 943, 1077, 1107
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 632, 751, 777
Bse1I ACTGG 3 cut(s) 116, 344, 351
Bse3DI GCAATG 1 cut(s) 174
Bse8I GATNNNNATC 1 cut(s) 537
BseDI CCNNGG 4 cut(s) 153, 406, 602, 795
BseGI GGATG 2 cut(s) 149, 1071
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 4 cut(s) 242, 632, 751, 777
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 1 cut(s) 1302
BseNI ACTGG 3 cut(s) 116, 344, 351
BseRI GAGGAG 2 cut(s) 965, 1072
BseXI GCAGC 3 cut(s) 527, 929, 1063
BshFI GGCC 2 cut(s) 794, 1271
BsiHKCI CYCGRG 2 cut(s) 306, 750
BsiSI CCGG 3 cut(s) 615, 913, 1300
BslI CCNNNNNNNGG 4 cut(s) 242, 632, 751, 777
BsmAI GTCTC 3 cut(s) 573, 753, 1118
BsmBI CGTCTC 1 cut(s) 753
BsnI GGCC 2 cut(s) 794, 1271
BsoBI CYCGRG 2 cut(s) 306, 750
Bsp1407I TGTACA 1 cut(s) 834
Bsp143I GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
BspACI CCGC 1 cut(s) 887
BspANI GGCC 2 cut(s) 794, 1271
BspCNI CTCAG 1 cut(s) 1301
BspHI TCATGA 2 cut(s) 6, 1053
BspLI GGNNCC 1 cut(s) 372
BspMAI CTGCAG 1 cut(s) 1121
BspMI ACCTGC 2 cut(s) 964, 1110
BspPI GGATC 2 cut(s) 644, 864
BsrDI GCAATG 1 cut(s) 174
BsrGI TGTACA 1 cut(s) 834
BsrI ACTGG 3 cut(s) 116, 344, 351
BssECI CCNNGG 4 cut(s) 153, 406, 602, 795
BssMI GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
BssT1I CCWWGG 2 cut(s) 406, 602
Bst4CI ACNGT 4 cut(s) 434, 594, 841, 1099
BstAPI GCANNNNNTGC 4 cut(s) 452, 521, 710, 923
BstAUI TGTACA 1 cut(s) 834
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 457, 1161
BstDEI CTNAG 1 cut(s) 1288
BstF5I GGATG 2 cut(s) 149, 1071
BstKTI GATC 8 cut(s) 6, 40, 400, 535, 556, 639, 690, 859
BstMAI GTCTC 3 cut(s) 573, 753, 1118
BstMBI GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
BstMWI GCNNNNNNNGC 9 cut(s) 248, 401, 452, 521, 701, 710, 767, 800, 923
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 614, 912
BstSFI CTRYAG 1 cut(s) 1117
BstV1I GCAGC 3 cut(s) 527, 929, 1063
BstV2I GAAGAC 2 cut(s) 737, 1269
BstX2I RGATCY 2 cut(s) 37, 856
BstYI RGATCY 2 cut(s) 37, 856
BsuRI GGCC 2 cut(s) 794, 1271
BtrI CACGTC 1 cut(s) 1346
BtsCI GGATG 2 cut(s) 149, 1071
BtsI GCAGTG 2 cut(s) 519, 921
BtsIMutI CAGTG 5 cut(s) 358, 519, 590, 921, 1104
BveI ACCTGC 2 cut(s) 964, 1110
Cac8I GCNNGC 2 cut(s) 457, 1161
CaiI CAGNNNCTG 2 cut(s) 521, 923
CciI TCATGA 2 cut(s) 6, 1053
Cfr13I GGNCC 1 cut(s) 223
Csp6I GTAC 1 cut(s) 835
CviAII CATG 8 cut(s) 7, 262, 557, 1054, 1174, 1183, 1210, 1267
CviQI GTAC 1 cut(s) 835
DdeI CTNAG 1 cut(s) 1288
DpnI GATC 8 cut(s) 5, 39, 399, 534, 555, 638, 689, 858
DpnII GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
EaeI YGGCCR 2 cut(s) 792, 1269
Eco130I CCWWGG 2 cut(s) 406, 602
Eco32I GATATC 2 cut(s) 83, 108
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 749
Eco72I CACGTG 1 cut(s) 629
Eco88I CYCGRG 2 cut(s) 306, 750
EcoRV GATATC 2 cut(s) 83, 108
EcoT14I CCWWGG 2 cut(s) 406, 602
ErhI CCWWGG 2 cut(s) 406, 602
Esp3I CGTCTC 1 cut(s) 753
FaeI CATG 8 cut(s) 10, 265, 560, 1057, 1177, 1186, 1213, 1270
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FatI CATG 8 cut(s) 6, 261, 556, 1053, 1173, 1182, 1209, 1266
FbaI TGATCA 2 cut(s) 3, 553
Fnu4HI GCNGC 4 cut(s) 516, 887, 918, 1077
FokI GGATG 2 cut(s) 136, 1078
Fsp4HI GCNGC 4 cut(s) 516, 887, 918, 1077
FspBI CTAG 3 cut(s) 383, 401, 525
GluI GCNGC 4 cut(s) 516, 887, 918, 1077
HaeIII GGCC 2 cut(s) 794, 1271
HapII CCGG 3 cut(s) 615, 913, 1300
Hin1II CATG 8 cut(s) 10, 265, 560, 1057, 1177, 1186, 1213, 1270
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 7 cut(s) 190, 815, 875, 1111, 1188, 1238, 1286
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 3 cut(s) 615, 913, 1300
HphI GGTGA 4 cut(s) 22, 870, 988, 1180
Hpy166II GTNNAC 3 cut(s) 313, 356, 837
Hpy188I TCNGA 7 cut(s) 47, 154, 214, 328, 546, 725, 1291
Hpy188III TCNNGA 6 cut(s) 7, 98, 825, 1025, 1054, 1327
Hpy8I GTNNAC 3 cut(s) 313, 356, 837
HpyAV CCTTC 5 cut(s) 4, 304, 781, 822, 1056
HpyCH4III ACNGT 4 cut(s) 434, 594, 841, 1099
HpyCH4IV ACGT 4 cut(s) 628, 707, 1103, 1345
HpyF10VI GCNNNNNNNGC 9 cut(s) 248, 401, 452, 521, 701, 710, 767, 800, 923
HpyF3I CTNAG 1 cut(s) 1288
HpySE526I ACGT 4 cut(s) 628, 707, 1103, 1345
Hsp92II CATG 8 cut(s) 10, 265, 560, 1057, 1177, 1186, 1213, 1270
Ksp22I TGATCA 2 cut(s) 3, 553
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
Lsp1109I GCAGC 3 cut(s) 527, 929, 1063
LweI GCATC 1 cut(s) 729
MaeI CTAG 3 cut(s) 383, 401, 525
MaeII ACGT 4 cut(s) 628, 707, 1103, 1345
MaeIII GTNAC 4 cut(s) 28, 655, 1093, 1099
MalI GATC 8 cut(s) 5, 39, 399, 534, 555, 638, 689, 858
MboI GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
MboII GAAGA 7 cut(s) 45, 697, 742, 821, 824, 1006, 1274
MfeI CAATTG 2 cut(s) 447, 1314
MflI RGATCY 2 cut(s) 37, 856
MlsI TGGCCA 1 cut(s) 1271
MluCI AATT 6 cut(s) 166, 227, 447, 1019, 1220, 1314
MluNI TGGCCA 1 cut(s) 1271
MlyI GAGTC 4 cut(s) 869, 1120, 1247, 1280
MmeI TCCRAC 3 cut(s) 83, 439, 956
Mox20I TGGCCA 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 1271
MseI TTAA 4 cut(s) 312, 494, 942, 1351
MslI CAYNNNNRTG 1 cut(s) 842
Msp20I TGGCCA 1 cut(s) 1271
MspI CCGG 3 cut(s) 615, 913, 1300
MspR9I CCNGG 2 cut(s) 616, 914
MunI CAATTG 2 cut(s) 447, 1314
MwoI GCNNNNNNNGC 9 cut(s) 248, 401, 452, 521, 701, 710, 767, 800, 923
NciI CCSGG 2 cut(s) 616, 914
NdeII GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
NlaIII CATG 8 cut(s) 10, 265, 560, 1057, 1177, 1186, 1213, 1270
NlaIV GGNNCC 1 cut(s) 372
NmeAIII GCCGAG 1 cut(s) 820
NmuCI GTSAC 2 cut(s) 28, 1099
NspI RCATGY 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 842
PaeR7I CTCGAG 1 cut(s) 306
PagI TCATGA 2 cut(s) 6, 1053
PaqCI CACCTGC 1 cut(s) 964
PciI ACATGT 1 cut(s) 261
PfeI GAWTC 3 cut(s) 190, 815, 1188
PflMI CCANNNNNTGG 2 cut(s) 242, 632
PkrI GCNGC 4 cut(s) 517, 888, 919, 1078
PleI GAGTC 4 cut(s) 869, 1119, 1246, 1280
PmaCI CACGTG 1 cut(s) 629
PmlI CACGTG 1 cut(s) 629
PpsI GAGTC 4 cut(s) 869, 1119, 1246, 1280
Ppu21I YACGTR 1 cut(s) 629
PscI ACATGT 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 942
PspCI CACGTG 1 cut(s) 629
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 223
PspXI VCTCGAGB 1 cut(s) 306
PstI CTGCAG 1 cut(s) 1121
PstNI CAGNNNCTG 2 cut(s) 521, 923
PsuI RGATCY 2 cut(s) 37, 856
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 4 cut(s) 312, 494, 942, 1351
SatI GCNGC 4 cut(s) 516, 887, 918, 1077
Sau3AI GATC 8 cut(s) 3, 37, 397, 532, 553, 636, 687, 856
Sau96I GGNCC 1 cut(s) 223
SchI GAGTC 4 cut(s) 869, 1120, 1247, 1280
ScrFI CCNGG 2 cut(s) 616, 914
SfaNI GCATC 1 cut(s) 729
SfcI CTRYAG 1 cut(s) 1117
Sfr274I CTCGAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 223
SlaI CTCGAG 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 842
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
Sse9I AATT 6 cut(s) 166, 227, 447, 1019, 1220, 1314
SsiI CCGC 1 cut(s) 887
SspMI CTAG 3 cut(s) 383, 401, 525
StyD4I CCNGG 2 cut(s) 614, 912
StyI CCWWGG 2 cut(s) 406, 602
TaaI ACNGT 4 cut(s) 434, 594, 841, 1099
TaiI ACGT 4 cut(s) 631, 710, 1106, 1348
TaqI TCGA 6 cut(s) 188, 307, 683, 690, 866, 1109
TasI AATT 6 cut(s) 166, 227, 447, 1019, 1220, 1314
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 889
TfiI GAWTC 3 cut(s) 190, 815, 1188
Tru1I TTAA 4 cut(s) 312, 494, 942, 1351
Tru9I TTAA 4 cut(s) 312, 494, 942, 1351
TscAI CASTG 5 cut(s) 358, 526, 597, 928, 1104
TseFI GTSAC 2 cut(s) 28, 1099
TseI GCWGC 3 cut(s) 515, 917, 1076
Tsp45I GTSAC 2 cut(s) 28, 1099
TspDTI ATGAA 6 cut(s) 23, 135, 256, 490, 1042, 1198
TspGWI ACGGA 1 cut(s) 851
TspRI CASTG 5 cut(s) 358, 526, 597, 928, 1104
Van91I CCANNNNNTGG 2 cut(s) 242, 632
VpaK11BI GGWCC 1 cut(s) 223
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 2 cut(s) 166, 1019
XceI RCATGY 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 306
XspI CTAG 3 cut(s) 383, 401, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.