Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003019

Physical Location & Seq

Genomic Coordinates Reverse (-)

11634222 .. 11639701

Gene Structure 5480 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1148400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1041 bp

ATGGTGGTGGCATCTCCAAACCCAACTCGCAACGAAAAGATCCAGGCCGTTGATCTTCCCATCATAGACCTCTCAGCCGAGAGGTCACAAGTGAGGAAGCTCATTGTCAAAGCTTGTGAGGACTATGGTTTCTTCAAGGTCATCAACCATGGCGTCCCAAAAGACATTATATCCAAAGTGGAGGAGCAAAGCCTCAGCTTCTTTGCCCAACCAGTGTCCGAGAAGCAAAGGGCAGGGCCTTCCGATCCCTTTGGCTATGGCTGCAAGAACATAGGCCTCAATGGTGATACGGGAGAGATTGAGTACCTTATTTTCAACACAAACCCAATTTCTATTGCTCAAAGATCCAAAACCATCTTAAATGAGCATACAAAATTCAGCTCGGCAGTGAGTGGATACATTGCAGCAGTTAGGGATTTGGCATGTGAGCTCTTGGATCTGATTGGTGAGGGATTGTGGGTCCCTGACACATCAGTCTTCAGTAGGCTGATCAGGGACGTCGACAGTGACTCGGTCTTCAGGCTCAATCACTACCCGCATCTGCACAACAAGAAGGACATCTGCAAGGATAATGATACGTCACCATCCTCCATCAGTAACATCAGCAAGGTAGGGTTTGGGGAGCATTCCGACCCTCAGCTCCTCACCCTCCTGAGGTCCAACGACGTGGGCGGCCTCCAAATCTCTCCACAAGATGGGGTGTGGGTCTCCGTCCCCTCTGACCCCACGGCCTATTGGGTTAATGTGGGTGACGTGTTGCAGGCCATGACAAATGGAAGATTTGTGAGCGTTAGACATAGAGCCTTAGCCAATTCATCAAAGTCTAGATTGTCAATGGCGTATTTTGCAGCTCCCTCCCTGAACGCATGTTTGAGTGTTCTTCCAGAAATGGTCACACCGGAGAAGCCTCGGCTCTACAAACCCTTCACTTGGGCTGAGTACAAGAAAACCACGCACTCTCTACGCCTCGGCGAAAACCGTCTTAACCTCTTCAGAATTTCTACCAATGATGAATGCTTCATAGATCAACCGCTGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008300 GO:0009056 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009987 GO:0010817 GO:0016020 GO:0016042 GO:0016054 GO:0016101 GO:0016103 GO:0016115 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0042445 GO:0042447 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0045487 GO:0045543 GO:0046395 GO:0050896 GO:0051213 GO:0052634 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

347

Amino Acids

38.46

Weight (kDa)

6.26

Isoelectric Point (pI)

42.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	19 - 83	8.8e-18	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	170 - 284	2.8e-25	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003019)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02400 AT1G02400
fragaria_vesca	FvH4_1g26760 FvH4_4g20730 FvH4_4g20730
malus_domestica	MD09G1286800.v1.1 MD13G1148400.v1.1 MD16G1148400.v1.1 MD17G1279100.v1.1
prunus_persica	Prupe.1G111900_v2.0.a1 Prupe.3G006700_v2.0.a1
pyrus_communis	pycom13g12780
rosa_chinensis	RchiOBHm_Chr3g0486971 RchiOBHm_Chr4g0426161
rosa_laevigata	RLG00000007314 RLG00000023043
rosa_multiflora	Rmu_co8169984.1_g000001 Rmu_sc0000501.1_g000031 Rmu_sc0000730.1_g000008 Rmu_sc0000869.1_g000024 Rmu_sc0001365.1_g000007 Rmu_sc0020668.1_g000001
rosa_roxburghii	Rroxscaffold_5G00368180 Rroxscaffold_6G00395310 Rroxscaffold_6G00395320
rosa_rugosa	Rorug03G0228800 Rorug03G0228800 Rorug04G0209300
rosa_samantha	Rh3AG278300 Rh3BG313900 Rh3CG312500 Rh3DG308800 Rh4AG266000 Rh4BG272100 Rh4CG285100 Rh4DG268200
rosa_wichuraiana	Rw3G024700 Rw4G023000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	473
AatII	GACGTC	1 cut(s)	501
AccB7I	CCANNNNNTGG	1 cut(s)	695
AccI	GTMKAC	1 cut(s)	501
AciI	CCGC	3 cut(s)	536, 672, 1031
AclWI	GGATC	4 cut(s)	34, 239, 339, 444
AcsI	RAATTY	2 cut(s)	374, 996
AcuI	CTGAAG	3 cut(s)	463, 502, 976
AcyI	GRCGYC	2 cut(s)	153, 498
AfaI	GTAC	2 cut(s)	305, 941
AfiI	CCNNNNNNNGG	3 cut(s)	654, 695, 930
AflIII	ACRYGT	1 cut(s)	753
AgsI	TTSAA	2 cut(s)	136, 316
AjiI	CACGTC	2 cut(s)	667, 754
AjnI	CCWGG	1 cut(s)	42
AluBI	AGCT	7 cut(s)	100, 113, 198, 381, 430, 640, 851
AluI	AGCT	7 cut(s)	100, 113, 198, 381, 430, 640, 851
Alw21I	GWGCWC	1 cut(s)	432
Alw26I	GTCTC	1 cut(s)	712
AlwI	GGATC	4 cut(s)	34, 239, 339, 444
AoxI	GGCC	6 cut(s)	45, 236, 274, 673, 729, 762
ApeKI	GCWGC	3 cut(s)	261, 404, 848
ApoI	RAATTY	2 cut(s)	374, 996
AspS9I	GGNCC	3 cut(s)	236, 460, 657
AsuHPI	GGTGA	5 cut(s)	296, 458, 573, 637, 761
AvaII	GGWCC	2 cut(s)	460, 657
AxyI	CCTNAGG	1 cut(s)	653
BanII	GRGCYC	1 cut(s)	432
BbsI	GAAGAC	2 cut(s)	469, 508
Bbv12I	GWGCWC	1 cut(s)	432
BbvCI	CCTCAGC	2 cut(s)	194, 636
BbvI	GCAGC	3 cut(s)	248, 416, 860
BccI	CCATC	5 cut(s)	68, 362, 592, 599, 689
BceAI	ACGGC	2 cut(s)	32, 744
BciT130I	CCWGG	1 cut(s)	44
BciVI	GTATCC	1 cut(s)	389
BclI	TGATCA	1 cut(s)	489
BcoDI	GTCTC	1 cut(s)	712
BfaI	CTAG	1 cut(s)	825
BfuI	GTATCC	1 cut(s)	389
BisI	GCNGC	4 cut(s)	262, 405, 673, 849
BlsI	GCNGC	4 cut(s)	263, 406, 674, 850
Bme1390I	CCNGG	1 cut(s)	44
Bme18I	GGWCC	2 cut(s)	460, 657
BmgBI	CACGTC	2 cut(s)	667, 754
BmgT120I	GGNCC	3 cut(s)	236, 460, 657
BmiI	GGNNCC	2 cut(s)	461, 462
BmrFI	CCNGG	1 cut(s)	44
BmsI	GCATC	2 cut(s)	20, 547
BpiI	GAAGAC	2 cut(s)	469, 508
Bpu10I	CCTNAGC	3 cut(s)	194, 636, 805
BsaHI	GRCGYC	2 cut(s)	153, 498
BsaI	GGTCTC	1 cut(s)	712
BsaJI	CCNNGG	4 cut(s)	148, 726, 908, 967
BsaWI	WCCGGW	1 cut(s)	898
Bsc4I	CCNNNNNNNGG	3 cut(s)	654, 695, 930
Bse1I	ACTGG	1 cut(s)	212
Bse21I	CCTNAGG	1 cut(s)	653
Bse3DI	GCAATG	1 cut(s)	399
BseBI	CCWGG	1 cut(s)	44
BseDI	CCNNGG	4 cut(s)	148, 726, 908, 967
BseGI	GGATG	1 cut(s)	584
BseLI	CCNNNNNNNGG	3 cut(s)	654, 695, 930
BseMI	GCAATG	1 cut(s)	399
BseMII	CTCAG	5 cut(s)	87, 208, 644, 650, 927
BseNI	ACTGG	1 cut(s)	212
BseRI	GAGGAG	2 cut(s)	197, 632
BseXI	GCAGC	3 cut(s)	248, 416, 860
BsgI	GTGCAG	1 cut(s)	527
BshFI	GGCC	6 cut(s)	47, 238, 276, 675, 731, 764
BsiHKAI	GWGCWC	1 cut(s)	432
BsiSI	CCGG	1 cut(s)	899
BslFI	GGGAC	4 cut(s)	140, 446, 509, 698
BslI	CCNNNNNNNGG	3 cut(s)	654, 695, 930
BsmAI	GTCTC	1 cut(s)	712
BsmFI	GGGAC	4 cut(s)	140, 446, 509, 698
BsmI	GAATGC	2 cut(s)	625, 1019
BsnI	GGCC	6 cut(s)	47, 238, 276, 675, 731, 764
Bso31I	GGTCTC	1 cut(s)	712
Bsp1286I	GDGCHC	1 cut(s)	432
Bsp143I	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
Bsp19I	CCATGG	1 cut(s)	148
BspACI	CCGC	3 cut(s)	536, 672, 1031
BspANI	GGCC	6 cut(s)	47, 238, 276, 675, 731, 764
BspCNI	CTCAG	5 cut(s)	86, 207, 645, 649, 928
BspLI	GGNNCC	2 cut(s)	461, 462
BspPI	GGATC	4 cut(s)	34, 239, 339, 444
BspTNI	GGTCTC	1 cut(s)	712
BsrDI	GCAATG	1 cut(s)	399
BsrI	ACTGG	1 cut(s)	212
BssECI	CCNNGG	4 cut(s)	148, 726, 908, 967
BssMI	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
BssNI	GRCGYC	2 cut(s)	153, 498
BssT1I	CCWWGG	1 cut(s)	148
Bst2UI	CCWGG	1 cut(s)	44
Bst4CI	ACNGT	2 cut(s)	506, 980
Bst6I	CTCTTC	1 cut(s)	995
BstACI	GRCGYC	2 cut(s)	153, 498
BstC8I	GCNNGC	1 cut(s)	762
BstDEI	CTNAG	6 cut(s)	73, 194, 636, 653, 805, 936
BstDSI	CCRYGG	2 cut(s)	148, 726
BstF5I	GGATG	1 cut(s)	584
BstKTI	GATC	7 cut(s)	42, 55, 247, 347, 439, 492, 1027
BstMAI	GTCTC	1 cut(s)	712
BstMBI	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
BstMWI	GCNNNNNNNGC	2 cut(s)	261, 845
BstNI	CCWGG	1 cut(s)	44
BstNSI	RCATGY	2 cut(s)	426, 870
BstSCI	CCNGG	1 cut(s)	42
BstV1I	GCAGC	3 cut(s)	248, 416, 860
BstV2I	GAAGAC	2 cut(s)	469, 508
BstX2I	RGATCY	3 cut(s)	39, 344, 436
BstXI	CCANNNNNNTGG	1 cut(s)	667
BstYI	RGATCY	3 cut(s)	39, 344, 436
Bsu36I	CCTNAGG	1 cut(s)	653
BsuI	GTATCC	1 cut(s)	389
BsuRI	GGCC	6 cut(s)	47, 238, 276, 675, 731, 764
BtgI	CCRYGG	2 cut(s)	148, 726
BtrI	CACGTC	2 cut(s)	667, 754
BtsCI	GGATG	1 cut(s)	584
BtsI	GCAGTG	1 cut(s)	393
BtsIMutI	CAGTG	3 cut(s)	219, 393, 511
Cac8I	GCNNGC	1 cut(s)	762
Cfr13I	GGNCC	3 cut(s)	236, 460, 657
CseI	GACGC	1 cut(s)	142
Csp6I	GTAC	2 cut(s)	304, 940
CviAII	CATG	4 cut(s)	149, 423, 766, 867
CviQI	GTAC	2 cut(s)	304, 940
DdeI	CTNAG	6 cut(s)	73, 194, 636, 653, 805, 936
DpnI	GATC	7 cut(s)	41, 54, 246, 346, 438, 491, 1026
DpnII	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
DrdI	GACNNNNNNGTC	1 cut(s)	473
DseDI	GACNNNNNNGTC	1 cut(s)	473
Eam1104I	CTCTTC	1 cut(s)	995
EarI	CTCTTC	1 cut(s)	995
Ecl136II	GAGCTC	1 cut(s)	430
Eco130I	CCWWGG	1 cut(s)	148
Eco147I	AGGCCT	1 cut(s)	276
Eco24I	GRGCYC	1 cut(s)	432
Eco31I	GGTCTC	1 cut(s)	712
Eco47I	GGWCC	2 cut(s)	460, 657
Eco53kI	GAGCTC	1 cut(s)	430
Eco57I	CTGAAG	3 cut(s)	463, 502, 976
Eco81I	CCTNAGG	1 cut(s)	653
EcoICRI	GAGCTC	1 cut(s)	430
EcoO109I	RGGNCCY	2 cut(s)	236, 460
EcoRII	CCWGG	1 cut(s)	42
EcoT14I	CCWWGG	1 cut(s)	148
EcoT38I	GRGCYC	1 cut(s)	432
ErhI	CCWWGG	1 cut(s)	148
FaeI	CATG	4 cut(s)	152, 426, 769, 870
FaqI	GGGAC	4 cut(s)	140, 446, 509, 698
FatI	CATG	4 cut(s)	148, 422, 765, 866
FauI	CCCGC	1 cut(s)	543
FbaI	TGATCA	1 cut(s)	489
FblI	GTMKAC	1 cut(s)	501
Fnu4HI	GCNGC	4 cut(s)	262, 405, 673, 849
FokI	GGATG	1 cut(s)	571
FriOI	GRGCYC	1 cut(s)	432
Fsp4HI	GCNGC	4 cut(s)	262, 405, 673, 849
FspBI	CTAG	1 cut(s)	825
GluI	GCNGC	4 cut(s)	262, 405, 673, 849
HaeIII	GGCC	6 cut(s)	47, 238, 276, 675, 731, 764
HapII	CCGG	1 cut(s)	899
HgaI	GACGC	1 cut(s)	142
Hin1I	GRCGYC	2 cut(s)	153, 498
Hin1II	CATG	4 cut(s)	152, 426, 769, 870
HincII	GTYRAC	1 cut(s)	502
HindII	GTYRAC	1 cut(s)	502
HindIII	AAGCTT	1 cut(s)	111
HinfI	GANTC	1 cut(s)	509
HpaII	CCGG	1 cut(s)	899
HphI	GGTGA	5 cut(s)	296, 458, 573, 637, 761
Hpy166II	GTNNAC	1 cut(s)	502
Hpy188I	TCNGA	6 cut(s)	220, 244, 441, 631, 721, 995
Hpy188III	TCNNGA	3 cut(s)	652, 825, 884
Hpy8I	GTNNAC	1 cut(s)	502
Hpy99I	CGWCG	2 cut(s)	503, 668
HpyAV	CCTTC	3 cut(s)	249, 547, 934
HpyCH4III	ACNGT	2 cut(s)	506, 980
HpyCH4IV	ACGT	4 cut(s)	498, 578, 666, 753
HpyCH4V	TGCA	6 cut(s)	264, 404, 544, 564, 760, 848
HpyF10VI	GCNNNNNNNGC	2 cut(s)	261, 845
HpyF3I	CTNAG	6 cut(s)	73, 194, 636, 653, 805, 936
HpySE526I	ACGT	4 cut(s)	498, 578, 666, 753
Hsp92I	GRCGYC	2 cut(s)	153, 498
Hsp92II	CATG	4 cut(s)	152, 426, 769, 870
KflI	GGGWCCC	1 cut(s)	460
Ksp22I	TGATCA	1 cut(s)	489
Kzo9I	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
LmnI	GCTCC	4 cut(s)	184, 622, 645, 856
Lsp1109I	GCAGC	3 cut(s)	248, 416, 860
LweI	GCATC	2 cut(s)	20, 547
MaeI	CTAG	1 cut(s)	825
MaeII	ACGT	4 cut(s)	498, 578, 666, 753
MaeIII	GTNAC	6 cut(s)	84, 506, 579, 596, 749, 892
MalI	GATC	7 cut(s)	41, 54, 246, 346, 438, 491, 1026
MboI	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
MboII	GAAGA	7 cut(s)	47, 124, 469, 508, 789, 872, 982
MflI	RGATCY	3 cut(s)	39, 344, 436
MhlI	GDGCHC	1 cut(s)	432
MluCI	AATT	4 cut(s)	327, 374, 811, 996
MlyI	GAGTC	1 cut(s)	503
MmeI	TCCRAC	2 cut(s)	654, 684
MseI	TTAA	3 cut(s)	359, 741, 984
MspA1I	CMGCKG	1 cut(s)	1033
MspI	CCGG	1 cut(s)	899
MspR9I	CCNGG	1 cut(s)	44
Mva1269I	GAATGC	2 cut(s)	625, 1019
MvaI	CCWGG	1 cut(s)	44
MwoI	GCNNNNNNNGC	2 cut(s)	261, 845
NcoI	CCATGG	1 cut(s)	148
NdeII	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
NlaIII	CATG	4 cut(s)	152, 426, 769, 870
NlaIV	GGNNCC	2 cut(s)	461, 462
NmeAIII	GCCGAG	4 cut(s)	103, 362, 889, 948
NmuCI	GTSAC	5 cut(s)	84, 506, 579, 749, 892
NspI	RCATGY	2 cut(s)	426, 870
PceI	AGGCCT	1 cut(s)	276
PctI	GAATGC	2 cut(s)	625, 1019
PflFI	GACNNNGTC	1 cut(s)	512
PflMI	CCANNNNNTGG	1 cut(s)	695
PkrI	GCNGC	4 cut(s)	263, 406, 674, 850
PleI	GAGTC	1 cut(s)	503
PpsI	GAGTC	1 cut(s)	503
PpuMI	RGGWCCY	1 cut(s)	460
Psp124BI	GAGCTC	1 cut(s)	432
Psp5II	RGGWCCY	1 cut(s)	460
Psp6I	CCWGG	1 cut(s)	42
PspGI	CCWGG	1 cut(s)	42
PspN4I	GGNNCC	2 cut(s)	461, 462
PspPI	GGNCC	3 cut(s)	236, 460, 657
PspPPI	RGGWCCY	1 cut(s)	460
PsuI	RGATCY	3 cut(s)	39, 344, 436
PsyI	GACNNNGTC	1 cut(s)	512
RsaI	GTAC	2 cut(s)	305, 941
RsaNI	GTAC	2 cut(s)	304, 940
SacI	GAGCTC	1 cut(s)	432
SalI	GTCGAC	1 cut(s)	500
SaqAI	TTAA	3 cut(s)	359, 741, 984
SatI	GCNGC	4 cut(s)	262, 405, 673, 849
Sau3AI	GATC	7 cut(s)	39, 52, 244, 344, 436, 489, 1024
Sau96I	GGNCC	3 cut(s)	236, 460, 657
SchI	GAGTC	1 cut(s)	503
ScrFI	CCNGG	1 cut(s)	44
SduI	GDGCHC	1 cut(s)	432
SfaNI	GCATC	2 cut(s)	20, 547
SinI	GGWCC	2 cut(s)	460, 657
Sse9I	AATT	4 cut(s)	327, 374, 811, 996
SseBI	AGGCCT	1 cut(s)	276
SsiI	CCGC	3 cut(s)	536, 672, 1031
SspMI	CTAG	1 cut(s)	825
SstI	GAGCTC	1 cut(s)	432
StuI	AGGCCT	1 cut(s)	276
StyD4I	CCNGG	1 cut(s)	42
StyI	CCWWGG	1 cut(s)	148
TaaI	ACNGT	2 cut(s)	506, 980
TaiI	ACGT	4 cut(s)	501, 581, 669, 756
TaqI	TCGA	1 cut(s)	501
TaqII	GACCGA	1 cut(s)	502
TasI	AATT	4 cut(s)	327, 374, 811, 996
TatI	WGTACW	1 cut(s)	939
TauI	GCSGC	1 cut(s)	675
Tru1I	TTAA	3 cut(s)	359, 741, 984
Tru9I	TTAA	3 cut(s)	359, 741, 984
TscAI	CASTG	3 cut(s)	219, 393, 511
TseFI	GTSAC	5 cut(s)	84, 506, 579, 749, 892
TseI	GCWGC	3 cut(s)	261, 404, 848
Tsp45I	GTSAC	5 cut(s)	84, 506, 579, 749, 892
TspDTI	ATGAA	3 cut(s)	804, 1009, 1026
TspGWI	ACGGA	1 cut(s)	700
TspRI	CASTG	3 cut(s)	219, 393, 511
Tth111I	GACNNNGTC	1 cut(s)	512
Van91I	CCANNNNNTGG	1 cut(s)	695
VpaK11BI	GGWCC	2 cut(s)	460, 657
XapI	RAATTY	2 cut(s)	374, 996
XbaI	TCTAGA	1 cut(s)	824
XceI	RCATGY	2 cut(s)	426, 870
XmiI	GTMKAC	1 cut(s)	501
XspI	CTAG	1 cut(s)	825
ZraI	GACGTC	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.