Rh4AG266000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003019
Physical Location & Seq
Forward (+)
58204155 .. 58207913
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG266000.1

Sequence Viewer

Length: 681 bp
ATGCTTATGCTTCTGCTGCTGGTAGAAGTACTTGTGAGTTTGAGTATACGTACTGGACTGATAGACTCGACTTTGTTTGGGGGGTGGGGGGCCTGGTGCAGCGCTGCAGTGAGTGATTACATTGAGGCAGTTAGTGATTTGGCATGTGAGCTTTTGGAGGGGATCGCCGAGGGACTGTGGGTCCCGGACACGTCGGTGTTCAGCAGGCTCATCAGGGACGTCGAGAGCGACTCTGTTTTCAGGCTCAATTACTACCCGCCCACGTCATCGTCGCCAACCTCCAAGGTCGGCTTCGGGGAGCACACTGACCCTCAAATTCTGACTCTGCTCCGATCCAACGACGTCGGAGGCCTCCAAATCTCACCGCAGGATGGTGTATGGGTCCCGGTGCCGTCCGACCCCGCTGCCTTCTGGGTTAATGTGGGTGACGTGCTGCAGGCAATGACAAATGAAAGATTTGTGAGCGTAAAACACAGAGCCTTAACCAACTCCTTCAAGTCAAGAATGTCAATGGCGTATTTCGCAGGCCCTCCTCTAAATGCAAGCATGAGAGCTCTCCCAGAAATGGTCACACCTGAGCACCCTCTCATTTACAAAGCATTCACTTGGGCCGAGTACAAGAAAACGACTTACTCTCTGCGCCTCAGTGACTCTCGCCTTAATCTCTTCAGAATCTCTTGA

Protein Analysis

226

Amino Acids

25.03

Weight (kDa)

5.21

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 77 - 177 3.6e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 222, 345
AccB1I GGYRCC 1 cut(s) 388
AccI GTMKAC 1 cut(s) 46
AciI CCGC 3 cut(s) 257, 365, 402
AclWI GGATC 2 cut(s) 170, 327
AcsI RAATTY 1 cut(s) 315
AcuI CTGAAG 1 cut(s) 652
AcyI GRCGYC 2 cut(s) 219, 342
AfaI GTAC 3 cut(s) 30, 52, 617
AfeI AGCGCT 1 cut(s) 103
AfiI CCNNNNNNNGG 2 cut(s) 371, 565
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 496
AhdI GACNNNNNGTC 1 cut(s) 179
AjiI CACGTC 3 cut(s) 192, 264, 430
AjnI CCWGG 1 cut(s) 92
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AleI CACNNNNGTG 1 cut(s) 194
AluBI AGCT 2 cut(s) 151, 554
AluI AGCT 2 cut(s) 151, 554
Alw21I GWGCWC 3 cut(s) 303, 556, 582
AlwI GGATC 2 cut(s) 170, 327
Aor51HI AGCGCT 1 cut(s) 103
AoxI GGCC 4 cut(s) 90, 349, 526, 609
ApeKI GCWGC 5 cut(s) 16, 99, 104, 404, 433
ApoI RAATTY 1 cut(s) 315
AspLEI GCGC 2 cut(s) 104, 642
AspS9I GGNCC 5 cut(s) 90, 181, 382, 527, 609
AsuC2I CCSGG 2 cut(s) 185, 386
AsuHPI GGTGA 2 cut(s) 354, 437
AvaII GGWCC 2 cut(s) 181, 382
BanI GGYRCC 1 cut(s) 388
BanII GRGCYC 1 cut(s) 556
Bbv12I GWGCWC 3 cut(s) 303, 556, 582
BbvI GCAGC 5 cut(s) 3, 91, 111, 391, 420
BccI CCATC 1 cut(s) 365
BceAI ACGGC 1 cut(s) 376
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 2 cut(s) 185, 386
BfmI CTRYAG 2 cut(s) 105, 434
BfoI RGCGCY 1 cut(s) 105
BisI GCNGC 5 cut(s) 17, 100, 105, 405, 434
BlsI GCNGC 5 cut(s) 18, 101, 106, 406, 435
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 3 cut(s) 94, 185, 386
Bme18I GGWCC 2 cut(s) 181, 382
BmeRI GACNNNNNGTC 1 cut(s) 179
BmgBI CACGTC 3 cut(s) 192, 264, 430
BmgT120I GGNCC 5 cut(s) 90, 181, 382, 527, 609
BmiI GGNNCC 6 cut(s) 91, 182, 183, 383, 384, 390
BmrFI CCNGG 3 cut(s) 94, 185, 386
BplI GAGNNNNNCTC 2 cut(s) 215, 247
Bpu10I CCTNAGC 1 cut(s) 576
BpuMI CCSGG 2 cut(s) 185, 386
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 219, 342
BsaJI CCNNGG 2 cut(s) 168, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 565
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 168, 282
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 2 cut(s) 371, 565
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 2 cut(s) 567, 658
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 5 cut(s) 3, 91, 111, 391, 420
BsgI GTGCAG 1 cut(s) 118
BshFI GGCC 4 cut(s) 92, 351, 528, 611
BshNI GGYRCC 1 cut(s) 388
BsiHKAI GWGCWC 3 cut(s) 303, 556, 582
BsiSI CCGG 2 cut(s) 185, 386
BslFI GGGAC 4 cut(s) 167, 186, 230, 368
BslI CCNNNNNNNGG 2 cut(s) 371, 565
BsmFI GGGAC 4 cut(s) 167, 186, 230, 368
BsmI GAATGC 1 cut(s) 599
BsnI GGCC 4 cut(s) 92, 351, 528, 611
Bsp1286I GDGCHC 3 cut(s) 303, 556, 582
Bsp143I GATC 2 cut(s) 162, 332
BspACI CCGC 3 cut(s) 257, 365, 402
BspANI GGCC 4 cut(s) 92, 351, 528, 611
BspCNI CTCAG 2 cut(s) 568, 657
BspLI GGNNCC 6 cut(s) 91, 182, 183, 383, 384, 390
BspMAI CTGCAG 2 cut(s) 109, 438
BspPI GGATC 2 cut(s) 170, 327
BspT107I GGYRCC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 447
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 168, 282
BssMI GATC 2 cut(s) 162, 332
BssNAI GTATAC 1 cut(s) 47
BssNI GRCGYC 2 cut(s) 219, 342
BssT1I CCWWGG 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 177
Bst6I CTCTTC 1 cut(s) 671
BstACI GRCGYC 2 cut(s) 219, 342
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 4 cut(s) 206, 438, 526, 544
BstDEI CTNAG 2 cut(s) 576, 644
BstF5I GGATG 1 cut(s) 376
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 642
BstKTI GATC 2 cut(s) 165, 335
BstMBI GATC 2 cut(s) 162, 332
BstMWI GCNNNNNNNGC 2 cut(s) 16, 521
BstNI CCWGG 1 cut(s) 94
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 3 cut(s) 92, 183, 384
BstSFI CTRYAG 2 cut(s) 105, 434
BstSNI TACGTA 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 3, 91, 111, 391, 420
BstZ17I GTATAC 1 cut(s) 47
BsuRI GGCC 4 cut(s) 92, 351, 528, 611
BtrI CACGTC 3 cut(s) 192, 264, 430
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 3 cut(s) 114, 303, 652
Cac8I GCNNGC 4 cut(s) 206, 438, 526, 544
CfoI GCGC 2 cut(s) 104, 642
Cfr13I GGNCC 5 cut(s) 90, 181, 382, 527, 609
Csp6I GTAC 3 cut(s) 29, 51, 616
CviAII CATG 2 cut(s) 144, 547
CviQI GTAC 3 cut(s) 29, 51, 616
DdeI CTNAG 2 cut(s) 576, 644
DpnI GATC 2 cut(s) 164, 334
DpnII GATC 2 cut(s) 162, 332
DriI GACNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 671
Eam1105I GACNNNNNGTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 671
Ecl136II GAGCTC 1 cut(s) 554
Eco105I TACGTA 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 556
Eco47I GGWCC 2 cut(s) 181, 382
Eco47III AGCGCT 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 652
EcoICRI GAGCTC 1 cut(s) 554
EcoO109I RGGNCCY 4 cut(s) 90, 181, 382, 527
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 2 cut(s) 147, 550
FaiI YATR 5 cut(s) 8, 47, 145, 379, 548
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FaqI GGGAC 4 cut(s) 167, 186, 230, 368
FatI CATG 2 cut(s) 143, 546
FauI CCCGC 2 cut(s) 264, 409
FblI GTMKAC 1 cut(s) 46
Fnu4HI GCNGC 5 cut(s) 17, 100, 105, 405, 434
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 5 cut(s) 17, 100, 105, 405, 434
GlaI GCGC 2 cut(s) 103, 641
GluI GCNGC 5 cut(s) 17, 100, 105, 405, 434
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 4 cut(s) 92, 351, 528, 611
HapII CCGG 2 cut(s) 185, 386
HhaI GCGC 2 cut(s) 104, 642
Hin1I GRCGYC 2 cut(s) 219, 342
Hin1II CATG 2 cut(s) 147, 550
Hin6I GCGC 2 cut(s) 102, 640
HinP1I GCGC 2 cut(s) 102, 640
HinfI GANTC 5 cut(s) 65, 230, 322, 650, 672
HpaII CCGG 2 cut(s) 185, 386
HphI GGTGA 2 cut(s) 354, 437
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 5 cut(s) 321, 332, 347, 397, 671
Hpy188III TCNNGA 3 cut(s) 223, 501, 678
Hpy8I GTNNAC 1 cut(s) 47
Hpy99I CGWCG 5 cut(s) 196, 224, 274, 344, 347
HpyAV CCTTC 2 cut(s) 418, 502
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4IV ACGT 6 cut(s) 49, 191, 219, 263, 342, 429
HpyCH4V TGCA 4 cut(s) 99, 107, 436, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 521
HpyF3I CTNAG 2 cut(s) 576, 644
HpySE526I ACGT 6 cut(s) 49, 191, 219, 263, 342, 429
Hsp92I GRCGYC 2 cut(s) 219, 342
Hsp92II CATG 2 cut(s) 147, 550
HspAI GCGC 2 cut(s) 102, 640
KflI GGGWCCC 2 cut(s) 181, 382
Kzo9I GATC 2 cut(s) 162, 332
LmnI GCTCC 2 cut(s) 298, 333
Lsp1109I GCAGC 5 cut(s) 3, 91, 111, 391, 420
MaeII ACGT 6 cut(s) 49, 191, 219, 263, 342, 429
MaeIII GTNAC 3 cut(s) 425, 568, 647
MalI GATC 2 cut(s) 164, 334
MboI GATC 2 cut(s) 162, 332
MboII GAAGA 1 cut(s) 658
MhlI GDGCHC 3 cut(s) 303, 556, 582
MluCI AATT 2 cut(s) 247, 315
MlyI GAGTC 4 cut(s) 59, 224, 316, 644
MmeI TCCRAC 3 cut(s) 325, 360, 420
MseI TTAA 3 cut(s) 417, 482, 660
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 185, 386
MspR9I CCNGG 3 cut(s) 94, 185, 386
Mva1269I GAATGC 1 cut(s) 599
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 16, 521
NciI CCSGG 2 cut(s) 185, 386
NdeII GATC 2 cut(s) 162, 332
NlaIII CATG 2 cut(s) 147, 550
NlaIV GGNNCC 6 cut(s) 91, 182, 183, 383, 384, 390
NmeAIII GCCGAG 2 cut(s) 193, 637
NmuCI GTSAC 3 cut(s) 425, 568, 647
NspI RCATGY 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 194
PceI AGGCCT 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 599
PfeI GAWTC 1 cut(s) 672
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 5 cut(s) 18, 101, 106, 406, 435
PleI GAGTC 4 cut(s) 59, 224, 316, 644
PpsI GAGTC 4 cut(s) 59, 224, 316, 644
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 2 cut(s) 181, 382
Psp124BI GAGCTC 1 cut(s) 556
Psp5II RGGWCCY 2 cut(s) 181, 382
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 6 cut(s) 91, 182, 183, 383, 384, 390
PspPI GGNCC 5 cut(s) 90, 181, 382, 527, 609
PspPPI RGGWCCY 2 cut(s) 181, 382
PstI CTGCAG 2 cut(s) 109, 438
RsaI GTAC 3 cut(s) 30, 52, 617
RsaNI GTAC 3 cut(s) 29, 51, 616
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 1 cut(s) 556
SaqAI TTAA 3 cut(s) 417, 482, 660
SatI GCNGC 5 cut(s) 17, 100, 105, 405, 434
Sau3AI GATC 2 cut(s) 162, 332
Sau96I GGNCC 5 cut(s) 90, 181, 382, 527, 609
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 4 cut(s) 59, 224, 316, 644
ScrFI CCNGG 3 cut(s) 94, 185, 386
SduI GDGCHC 3 cut(s) 303, 556, 582
SfcI CTRYAG 2 cut(s) 105, 434
SinI GGWCC 2 cut(s) 181, 382
SmiMI CAYNNNNRTG 1 cut(s) 194
SnaBI TACGTA 1 cut(s) 50
Sse9I AATT 2 cut(s) 247, 315
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 3 cut(s) 257, 365, 402
SstI GAGCTC 1 cut(s) 556
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 92, 183, 384
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 177
TaiI ACGT 6 cut(s) 52, 194, 222, 266, 345, 432
TaqI TCGA 2 cut(s) 68, 222
TasI AATT 2 cut(s) 247, 315
TatI WGTACW 2 cut(s) 28, 615
TfiI GAWTC 1 cut(s) 672
Tru1I TTAA 3 cut(s) 417, 482, 660
Tru9I TTAA 3 cut(s) 417, 482, 660
TscAI CASTG 3 cut(s) 114, 310, 652
TseFI GTSAC 3 cut(s) 425, 568, 647
TseI GCWGC 5 cut(s) 16, 99, 104, 404, 433
Tsp45I GTSAC 3 cut(s) 425, 568, 647
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 3 cut(s) 114, 310, 652
VpaK11BI GGWCC 2 cut(s) 181, 382
XapI RAATTY 1 cut(s) 315
XceI RCATGY 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 46
ZraI GACGTC 2 cut(s) 220, 343
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.