Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003731

Physical Location & Seq

Genomic Coordinates Forward (+)

12160134 .. 12177075

Gene Structure 16942 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1156100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5136 bp

ATGAAACCTTTGAGCTTTCTCAGTAAGGTGCATGACAAGAATCAACCTGAGGATCTAGAGACAACTTATATAACTCATGCTAAGGAGCCTGATGCGGATATTGACATCAGAGAAGTGTATTTTCTTATCATGCATTTTCTGTCAGCTGGCCCATGCCATAGAACTTGTGGACAGTTGTGGAACGAACTTCTTGAACATCAACTTTTACCTAGAAGATATCATGCTTGGTATTCAAGGAAGGGTATGCACAGCGAGGATGAAAATGATGATGGGAAGTCATTTCCTTTAAATTACAATATGCTGGTGCACAGGTATCCTCACATTGAAAATGACCATTTGGTAAAGCTTTTGAAGCAATTGATTTCAAGTGCAGCCCCACCATCAAGAGGTACGAGTGGTGGAAATGCTCCAAATGCAGCTGATGTACCTACTCTCTTGGGACGTGGTTCATTTTCGCTTCTGACCTATGAAAGGGATCAAATACACAATGAAATGAAACCCCCTCCTGCTCACATGCGTTGGCCTCACATGAAAGCTCATCAGGTTCAAGGGCTAAGGTTGAGAGAAATTGGGGGAGGTTTCACCCGACATCACCGTGCACCGTCTATTCGTGCTGCATCTTATGCCATTGCAAAGCCCTCAACCATGTTTGAGAAGATGCAATTTACCAAGAGGATAAGGGGCCATCGAAACTCTGTCTACTGTGCAACATTTGATTGCTCAGGCAGGTATGTTATTACCGGTTCAGACGATCGCCTAGTGAAGATCTGGTTAATGGAAACTGCATTTTGCTTAGCCAGCTGCCGTGGACATGAAGGAGATATCACGGATCTGGCTGTGAGTTCCAATAATGCTTTGGTGGCATCCTCCTCAAATGACCATATGATTCGAGTATGGCGCTTGCCAGACGGACTGCCAATTTCAGTATTGCGAGGACATACTGCAGCTGTTACTGCCATTACATTTAATCCCAGGCCAGGCTCTATGTACCACCTTCTATCTTCATCTGATGATGGAACATGTAGGATATGGGATGCCAGGAGTTCTCAAGTGAGTCCAAGGATATACATTCCAAGACCTTCAGATGCTGTAGTTGGGAGAAACAGTGGTCCATCTTCAAGCACTGTCACACAGGACCATCATATCTTCTGTTGTGCATTCAATGCTAATGGAACAGTCTTTGTCACCGGTAGCTCGGACACTCTTGCAAGGGTGTGGACTGCTTCTAAGCCAGGCTCGGATGAGTCGGACCAACCAAATCATGAGATTGATGTTTTATCTGGCCATGAGAATGATGTCAATTATGTACAATTTAGTGGTTGTGCTGGAGTATCTAGATTTATGGTAGCTGACACCTCAAAGGAAGAGAACACTCCCAAATTTAAAAATTCATGGTTCAATCATGACAATATAGTCACTTGTTCTCGCGATGGGAGTGCGATTATCTGGATTCCCAGATCACGAAGATCACATGGAAAAGCTGGTCGTTGGACACGGGCATATCATTTGAAAGTCCCACCTCCACCAATGCCGCCTCAGCCTTCCCGAGGAGGCCCTCGCCAGAGGATTCTTCCAACTCCTCGTGGTGTTAATATGATTACCTGGAGTCTTGACAATCGCTTTGTCCTTGCTGCTATCATGGATTGCAGAATATGTGTGTGGAATGCTTCTGATGGTAGCTTGGTGCATTCACTGACAGGTCATACTGAATCTACATATGTGCTGGATGTTCATCCTTTCAACCCCAGGATAGCAATGAGTGCTGGCTATGATGGAAAAACAATTGTCTGGGATATATGGGAAGGCATACCTATCCGGACATTTGAAACTTCCCACTACAAATTGGTAGATGGAAAGTTCTCACCGGATGGGACATCAATTATTCTCTCTGATGATGTTGGCCAATTGTATATATTGGACAGTGGCCAAGGGGAGTCCCAAGATGATGCACAATATGATCAGTTTTTTCTTGGTGATTATCGCCCACTCATTCACGATAGTTTTGGAAATGTTCTTGACCAGGAAACTCAGCTTCCAGCATACCGGCGGAATATGCAAGATCTACTTTGTGATTCAGGCATGATACCGTATGAAGAACCCTACCAGACTGCATACCAGAAGCGGCGGTTAGGAGCTCTGGGTGCTGAGTGGCGTCCTTCCTCTCTAAGACTTGCTGTTGGGCCTGACTTCAGTCTAGATCCGGATTACCAAATGTTGCCTTTGGCAGACTTGGATATATTGACTGAGCCTCTGCCAGAGTTTTTAGATGCAATGGATTGGGAACCAGAAAATGGAATACGTAGTGATGATACGGATTCAGAGTACCATATTACTGAAGATTATTCTACTGGAGGGGAGCAAGGAAGTTTAAGTTCAAATCCCTCTATCGATCCAGAGTGCAGTGAAGAAAGTGAGGATGAGGATGCTGAAATGGATGGACTTCGAAGATCAAAAAGGAAAAAACAAAAGGCTGACAACGAAGTCGTGACTTCCTCTGGTAGGTGTGTCAAAAGAAGGAATTTGGATGAGTGTGCTGCCAATTCATCCAGAAGTAACAGAGCAAGGAAATCAAGACATGGACGAAAAGCTTCAAGAAAGAAATGTTCCTCATCATTGAGACCTCAAAGAGCTGCTGCACGTAATGCTCTTAGTCTATTTTCTAAGATTACTGGAAGATCTGCAGATGGAGAAGATGAAGATGGATCGGAAGATGATATGTCAGGAAGTGAATCCCCACTTCAAGATTCCAACATTGAGAGTGATGAATCTGATAAACAATATCAGCAAGGCAAGCATTCAAAAGGAAAAGAGGTTTTACTCGATGAGTCTGAAGACATGGTTAAACCTATTGAAGAGTTTCCGGTAAATACGGGGAGCAGGAGGAGATTGGTGCTTAAATTACCAAGACAGGAGTCAAATAAGCTTGTGTCTAGAGAAAGCACAGTAAATAATCGTAGTAATGAGGATGATTTGGTTGACCAATCATATAGAGTACCTCAAGAAGCAAATGAGAATAATACAAGTCCTCAGGATCCAATGTCCTCCCCAGGTGACGAAAAATGCAGCAAATTTGAAACAGCAGTGAGTGGGCAGTCACAGAGAGTAGAAAATCATTTAAATTTCAAAGGGAGAATTAGTTGGGGAGGGTCCAGAGCACGAACAGCAAAGCGCCAGAGATCAGGAGAATTCATGTCATTGGACGCACTTGCCAGAGCCAGTGCAGTTGTTGGTCACAATGAAAAAGACTACACAAAACCTGAAAATGATTCTCCTTATTCAGAAAGCCAAAGGTTCAGGGATAAGATGGACACAGTGGCTATAGGGAATGAGAAAACCACTAGGGCTAGTACCTCCGATGGCCTTAATTGTGAAGTCAATGTTAAAGAGCATTCAGATTTTAGTGAATGCAAGGATCATGATCAATTGCCTAAATCTGTTCACATGGCTCCTTGGGATGCAAGCACTTCCTCTTGCCTTGATAAGGATGGGACTGGTTTCTCTTTCAAACAAAATGAGAAGTTCATACCTGTCTCAACGAAATTGAGGTCGAGGAGGATTTCAAGGGATCCTAGTCCTTGCAAACATGAAATTAACTCTGTGGTAGAGAACATGGAAAATGATAGGACGATGGAACAGGGTCCAGTTGTGCCAGAGGATGATGGAACCCATAAATTTATTCCAGATCATAGATATGATGGTTCACGAGAATCAGACAATCAATCTGATAAGAATGTTATTGCTAGCACACATGAATCTGTCGAATCCAGTTTACAGAAAAACAAAATGTTCAGTGCTGTTTATAGAAGGGTGAAACCACATAGGGGTAGAAGTAACTTAGAAGGTGATAGTGGTGTCAACGGAGAAGGCTGTTCATATACTTCAAATACAAGCAACCACAATATCATTGCTGGAGACATTCAGGATGACACAACTGATGGAGGATGCAAGACACGGTCCATGGGGTTCAAGGCATCTGCATGTGATCCAAGTAGTCCAGATCATGATCCTAAGTTGGCGGAAGGACATGAGCCAGGTTATACATTGAACCGGAAAAGTTCCATGGATAAGTTTCAACTTGGGAATGAAGAACGGGGATCAAGTTCAAGGACGAAAGTTGGTTTGAGGTCTACTAGAAACAGGAGAAGCAGTTATCTCGACATGAGTCCAATGGATAAAAGGAAGCCGCATCAGTCAGGAAAGAAAGTATCATGGTTAATGTTGTCAGCGCATGAGGAGAGCTCAAGATATATCCCCCAGTTAGGGGACAAAGTTGTTTATCTGAGACAGGGGCATCAGGAGTATTTCGATCTGGGTGGATTGAGAGAAAACCCACCGTGGAGGTTTATAAAGGGGAGAATAAGAGCGGTCGAATTTTGCAATGTTCAAGAGCTTGAGTATTCTTCACGTGCAGGGTCTGGGGATAGCTGCTGTAAATTGACGATTCAATTTGTAGATCCTTCTTCTGATGTGTATGGTAAATCTTTTAAGATGACTCTGCCGGAAGTTACTGGTTTCCCAGACTTCATTGTTGAAAGAACTCGCTTCGACTCTTCCATGGAGAGAAATTGGTCATGTAGGGATCATTGCAAAGTCTGGTGGAAAAATGAGGGCGAGGATGACGGTAAGTGGTGGCATGGCAGGGTTAAGTCTAAACAGTTTAAATCGTTGGAGTTCCCAGACAGTCCTTGGGAGATGTACACTGTTCAGTACAAGAGTGATCCTAGTGATGTACAACTACATAGTCCTTGGGAGCTTTTTGATGAAATTACTCAATGGGAGCCGCCTCACATTGATGATAAAAGCAAAAAGAAGCTGCATTCGGCCTTTGCCAAACTAGAGCGATCAGCCGGAAGTCGTCAGGATCCTTTTGGGGTTGACAAACTGATGCAACTTCAACAGAAGCCAAAGTTTACGAACTGGTGTGTGGTTCCGATATCTCTTGATGTGATCCAGTCCAGGTTAGAGAACGACTACTATCGAAGCCTGGAAGCGCTGAAGCATGACTTTAAGGTCATGCTGGCAAATGCTGAAACCTATCTCGAGAGCAACGCGGTCAAACGCGCATCAGATAAAGAGCTTTTAGGGAAACTGAAATGCCTATCAGACTGGTTCACACAGACAATATCCTCCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008360 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019221 GO:0019222 GO:0022603 GO:0022604 GO:0023052 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0034097 GO:0038111 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071345 GO:0071840 GO:0080090 GO:0098760 GO:0098761 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_resp Bromodomain WD40

Protein Analysis

1712

Amino Acids

192.95

Weight (kDa)

6.27

Isoelectric Point (pI)

50.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRWD1_N	PF25437	26 - 123	1.2e-34	BRWD1-like, N-terminal domain
Beta-prop_TEP1_2nd	PF25047	216 - 348	2e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	217 - 304	2.8e-18	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	218 - 344	2.1e-08	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	218 - 351	1.3e-15	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	219 - 352	4.5e-27	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	220 - 350	2.6e-13	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	221 - 351	6e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	221 - 348	1.9e-21	WDR3 first beta-propeller domain
WDR55	PF24796	221 - 351	4.6e-07	WDR55
WD40	PF00400	222 - 257	1.6e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	223 - 300	6.3e-11	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	228 - 424	3.2e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2	PF23414	230 - 350	5.7e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	235 - 349	3.4e-15	Prp19 WD40 domain
Beta-prop_SCAP	PF24017	240 - 349	7.8e-09	SCAP Beta-propeller
WD40	PF00400	264 - 299	2.1e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	264 - 355	7.6e-08	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	269 - 351	7.5e-06	EIF3I
WD40_WDHD1_1st	PF24817	274 - 355	6.7e-12	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	288 - 351	4.4e-09	THOC3 beta-propeller domain
WD40	PF00400	306 - 345	4.4e-07	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	528 - 644	2.4e-12	WDR5 beta-propeller domain
WD40_Prp19	PF24814	529 - 640	1.3e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	529 - 628	8.9e-07	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	529 - 653	1.3e-14	THOC3 beta-propeller domain
BRWD_AD	PF25313	1414 - 1587	1.1e-55	BRWD/PHIP ancillary domain-like domain
Bromodomain	PF00439	1630 - 1685	1.4e-08	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49430 AT5G49430
fragaria_vesca	FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490
malus_domestica	MD13G1156100.v1.1 MD16G1157000.v1.1
prunus_persica	Prupe.1G121900_v2.0.a1 Prupe.1G121900_v2.0.a1
pyrus_communis	pycom13g13310 pycom13g13320 pycom13g13330 pycom13g13340 pycom13g13350 pycom16g13190 pycom16g13200
rosa_chinensis	RchiOBHm_Chr4g0424601
rosa_laevigata	RLG00000007452
rosa_multiflora	Rmu_co8472673.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366520
rosa_rugosa	Rorug04G0198100 Rorug04G0198200 Rorug04G0198300
rosa_samantha	Rh4AG254900 Rh4BG260400 Rh4CG271900 Rh4DG255100
rosa_wichuraiana	Rw4G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4346
AasI	GACNNNNNNGTC	3 cut(s)	1412, 2480, 5009
Acc36I	ACCTGC	1 cut(s)	717
AccB7I	CCANNNNNTGG	1 cut(s)	2291
AccBSI	CCGCTC	1 cut(s)	4364
AccI	GTMKAC	2 cut(s)	699, 4127
AccII	CGCG	3 cut(s)	1428, 5051, 5061
AccIII	TCCGGA	2 cut(s)	1815, 2200
AcoI	YGGCCR	3 cut(s)	1282, 1900, 1924
AcsI	RAATTY	8 cut(s)	1379, 1387, 2518, 3068, 3118, 3185, 3671, 4370
AcuI	CTGAAG	5 cut(s)	1065, 2173, 2355, 2850, 5015
AcvI	CACGTG	1 cut(s)	4406
AcyI	GRCGYC	1 cut(s)	2152
AfeI	AGCGCT	1 cut(s)	4992
AflIII	ACRYGT	1 cut(s)	1019
AgeI	ACCGGT	2 cut(s)	740, 1187
AjiI	CACGTC	1 cut(s)	443
Alw21I	GWGCWC	5 cut(s)	309, 601, 2137, 3157, 4241
Alw26I	GTCTC	5 cut(s)	53, 2611, 3535, 3906, 4276
Alw44I	GTGCAC	2 cut(s)	305, 597
AlwNI	CAGNNNCTG	2 cut(s)	1088, 4415
Ama87I	CYCGRG	2 cut(s)	1545, 5039
Aor13HI	TCCGGA	2 cut(s)	1815, 2200
Aor51HI	AGCGCT	1 cut(s)	4992
ApaLI	GTGCAC	2 cut(s)	305, 597
ApoI	RAATTY	8 cut(s)	1379, 1387, 2518, 3068, 3118, 3185, 3671, 4370
ArsI	GACNNNNNNTTYG	4 cut(s)	1604, 1636, 3530, 3562
AsiGI	ACCGGT	2 cut(s)	740, 1187
Asp700I	GAANNNNTTC	2 cut(s)	2587, 2856
AspLEI	GCGC	5 cut(s)	900, 3171, 4228, 4993, 5063
AsuHPI	GGTGA	8 cut(s)	574, 584, 1177, 1854, 1985, 3062, 3820, 3854
AsuII	TTCGAA	1 cut(s)	2443
AsuNHI	GCTAGC	1 cut(s)	3740
AvaI	CYCGRG	2 cut(s)	1545, 5039
AvaII	GGWCC	6 cut(s)	1109, 1135, 1249, 3147, 3638, 3954
AxyI	CCTNAGG	2 cut(s)	48, 3027
BaeGI	GKGCMC	2 cut(s)	309, 601
BalI	TGGCCA	3 cut(s)	1284, 1902, 1926
BamHI	GGATCC	3 cut(s)	3031, 3565, 4861
BanII	GRGCYC	2 cut(s)	2137, 4241
BauI	CACGAG	2 cut(s)	1581, 3702
BbrPI	CACGTG	1 cut(s)	4406
BbsI	GAAGAC	1 cut(s)	2838
Bbv12I	GWGCWC	5 cut(s)	309, 601, 2137, 3157, 4241
BbvCI	CCTCAGC	1 cut(s)	1536
BceAI	ACGGC	1 cut(s)	789
BcgI	CGANNNNNNTGC	4 cut(s)	1418, 1452, 4135, 4169
BciVI	GTATCC	1 cut(s)	324
BclI	TGATCA	2 cut(s)	1957, 3418
BcoDI	GTCTC	5 cut(s)	53, 2611, 3535, 3906, 4276
BfmI	CTRYAG	4 cut(s)	942, 1089, 2679, 3318
BfoI	RGCGCY	3 cut(s)	901, 3172, 4994
BfuAI	ACCTGC	1 cut(s)	717
BfuI	GTATCC	1 cut(s)	324
BglII	AGATCT	3 cut(s)	765, 2059, 2675
BlpI	GCTNAGC	1 cut(s)	793
Bme18I	GGWCC	6 cut(s)	1109, 1135, 1249, 3147, 3638, 3954
BmeT110I	CYCGRG	2 cut(s)	1545, 5039
BmgBI	CACGTC	1 cut(s)	443
BmrI	ACTGGG	1 cut(s)	4249
BmtI	GCTAGC	1 cut(s)	3744
BmuI	ACTGGG	1 cut(s)	4249
BoxI	GACNNNNGTC	3 cut(s)	2190, 2911, 4161
BpiI	GAAGAC	1 cut(s)	2838
BplI	GAGNNNNNCTC	2 cut(s)	4223, 4255
BpmI	CTGGAG	4 cut(s)	1347, 1624, 2370, 3930
Bpu10I	CCTNAGC	4 cut(s)	81, 554, 721, 1536
Bpu1102I	GCTNAGC	1 cut(s)	793
Bpu14I	TTCGAA	1 cut(s)	2443
BpuEI	CTTGAG	4 cut(s)	1032, 2982, 4225, 4412
Bsa29I	ATCGAT	1 cut(s)	2388
BsaAI	YACGTR	3 cut(s)	2300, 2639, 4406
BsaBI	GATNNNNATC	3 cut(s)	1731, 3417, 4002
BsaHI	GRCGYC	1 cut(s)	2152
BsaI	GGTCTC	1 cut(s)	2611
BsaWI	WCCGGW	7 cut(s)	740, 1187, 1815, 1864, 2200, 2860, 4047
BsaXI	ACNNNNNCTCC	6 cut(s)	1090, 1120, 3121, 3151, 4132, 4162
Bse118I	RCCGGY	3 cut(s)	740, 1187, 2043
Bse21I	CCTNAGG	2 cut(s)	48, 3027
Bse3DI	GCAATG	6 cut(s)	627, 1761, 2277, 3903, 4384, 4582
Bse8I	GATNNNNATC	3 cut(s)	1731, 3417, 4002
BseAI	TCCGGA	2 cut(s)	1815, 2200
BseCI	ATCGAT	1 cut(s)	2388
BseJI	GATNNNNATC	3 cut(s)	1731, 3417, 4002
BseMI	GCAATG	6 cut(s)	627, 1761, 2277, 3903, 4384, 4582
BseMII	CTCAG	9 cut(s)	34, 39, 735, 1550, 2042, 2136, 2235, 3041, 4271
BseRI	GAGGAG	6 cut(s)	859, 1563, 1569, 2896, 3565, 4247
BseSI	GKGCMC	2 cut(s)	309, 601
BsgI	GTGCAG	5 cut(s)	390, 2419, 2619, 3240, 4428
Bsh1236I	CGCG	3 cut(s)	1428, 5051, 5061
Bsh1285I	CGRYCG	2 cut(s)	754, 4368
BshTI	ACCGGT	2 cut(s)	740, 1187
BshVI	ATCGAT	1 cut(s)	2388
BsiEI	CGRYCG	2 cut(s)	754, 4368
BsiHKAI	GWGCWC	5 cut(s)	309, 601, 2137, 3157, 4241
BsiHKCI	CYCGRG	2 cut(s)	1545, 5039
BslFI	GGGAC	6 cut(s)	453, 1499, 1885, 1921, 3502, 4277
BsmAI	GTCTC	5 cut(s)	53, 2611, 3535, 3906, 4276
BsmFI	GGGAC	6 cut(s)	453, 1499, 1885, 1921, 3502, 4277
BsmI	GAATGC	7 cut(s)	1157, 1669, 1687, 2794, 3388, 3410, 4816
Bso31I	GGTCTC	1 cut(s)	2611
BsoBI	CYCGRG	2 cut(s)	1545, 5039
Bsp119I	TTCGAA	1 cut(s)	2443
Bsp1286I	GDGCHC	5 cut(s)	309, 601, 2137, 3157, 4241
Bsp13I	TCCGGA	2 cut(s)	1815, 2200
Bsp1407I	TGTACA	3 cut(s)	1306, 4695, 4729
Bsp1720I	GCTNAGC	1 cut(s)	793
Bsp19I	CCATGG	3 cut(s)	3957, 4059, 4554
Bsp68I	TCGCGA	1 cut(s)	1428
BspCNI	CTCAG	9 cut(s)	33, 40, 734, 1549, 2041, 2137, 2236, 3040, 4272
BspDI	ATCGAT	1 cut(s)	2388
BspEI	TCCGGA	2 cut(s)	1815, 2200
BspFNI	CGCG	3 cut(s)	1428, 5051, 5061
BspHI	TCATGA	4 cut(s)	1261, 1402, 3415, 4000
BspMAI	CTGCAG	2 cut(s)	946, 2683
BspMI	ACCTGC	1 cut(s)	717
BspOI	GCTAGC	1 cut(s)	3744
BspT104I	TTCGAA	1 cut(s)	2443
BspTNI	GGTCTC	1 cut(s)	2611
BsrBI	CCGCTC	1 cut(s)	4364
BsrDI	GCAATG	6 cut(s)	627, 1761, 2277, 3903, 4384, 4582
BsrFI	RCCGGY	3 cut(s)	740, 1187, 2043
BsrGI	TGTACA	3 cut(s)	1306, 4695, 4729
BssAI	RCCGGY	3 cut(s)	740, 1187, 2043
BssNI	GRCGYC	1 cut(s)	2152
BssSI	CACGAG	2 cut(s)	1581, 3702
BssT1I	CCWWGG	8 cut(s)	1058, 1927, 3449, 3957, 4059, 4554, 4685, 4745
Bst2BI	CACGAG	2 cut(s)	1581, 3702
Bst6I	CTCTTC	3 cut(s)	1359, 2847, 4555
BstACI	GRCGYC	1 cut(s)	2152
BstAPI	GCANNNNNTGC	4 cut(s)	623, 1163, 1760, 2642
BstAUI	TGTACA	3 cut(s)	1306, 4695, 4729
BstBAI	YACGTR	3 cut(s)	2300, 2639, 4406
BstBI	TTCGAA	1 cut(s)	2443
BstC8I	GCNNGC	8 cut(s)	148, 799, 902, 1765, 2792, 3460, 3742, 5019
BstDSI	CCRYGG	5 cut(s)	805, 3957, 4059, 4334, 4554
BstENI	CCTNNNNNAGG	4 cut(s)	469, 1545, 2497, 3479
BstFNI	CGCG	3 cut(s)	1428, 5051, 5061
BstH2I	RGCGCY	3 cut(s)	901, 3172, 4994
BstHHI	GCGC	5 cut(s)	900, 3171, 4228, 4993, 5063
BstMAI	GTCTC	5 cut(s)	53, 2611, 3535, 3906, 4276
BstMCI	CGRYCG	2 cut(s)	754, 4368
BstNSI	RCATGY	3 cut(s)	517, 1023, 3981
BstPAI	GACNNNNGTC	3 cut(s)	2190, 2911, 4161
BstSFI	CTRYAG	4 cut(s)	942, 1089, 2679, 3318
BstSLI	GKGCMC	2 cut(s)	309, 601
BstSNI	TACGTA	1 cut(s)	2300
BstUI	CGCG	3 cut(s)	1428, 5051, 5061
BstV2I	GAAGAC	1 cut(s)	2838
Bsu15I	ATCGAT	1 cut(s)	2388
Bsu36I	CCTNAGG	2 cut(s)	48, 3027
BsuI	GTATCC	1 cut(s)	324
BsuTUI	ATCGAT	1 cut(s)	2388
BtgI	CCRYGG	5 cut(s)	805, 3957, 4059, 4334, 4554
BtgZI	GCGATG	1 cut(s)	1443
BtrI	CACGTC	1 cut(s)	443
BtsI	GCAGTG	2 cut(s)	2407, 3087
BtuMI	TCGCGA	1 cut(s)	1428
BveI	ACCTGC	1 cut(s)	717
Cac8I	GCNNGC	8 cut(s)	148, 799, 902, 1765, 2792, 3460, 3742, 5019
CaiI	CAGNNNCTG	2 cut(s)	1088, 4415
CciI	TCATGA	4 cut(s)	1261, 1402, 3415, 4000
CfoI	GCGC	5 cut(s)	900, 3171, 4228, 4993, 5063
Cfr10I	RCCGGY	3 cut(s)	740, 1187, 2043
ClaI	ATCGAT	1 cut(s)	2388
CseI	GACGC	2 cut(s)	2141, 3209
CspAI	ACCGGT	2 cut(s)	740, 1187
DraI	TTTAAA	4 cut(s)	288, 1384, 3117, 4660
DrdI	GACNNNNNNGTC	3 cut(s)	1412, 2480, 5009
DseDI	GACNNNNNNGTC	3 cut(s)	1412, 2480, 5009
EaeI	YGGCCR	3 cut(s)	1282, 1900, 1924
Eam1104I	CTCTTC	3 cut(s)	1359, 2847, 4555
EarI	CTCTTC	3 cut(s)	1359, 2847, 4555
EciI	GGCGGA	2 cut(s)	2062, 4031
Ecl136II	GAGCTC	2 cut(s)	2135, 4239
Eco105I	TACGTA	1 cut(s)	2300
Eco130I	CCWWGG	8 cut(s)	1058, 1927, 3449, 3957, 4059, 4554, 4685, 4745
Eco24I	GRGCYC	2 cut(s)	2137, 4241
Eco31I	GGTCTC	1 cut(s)	2611
Eco32I	GATATC	3 cut(s)	218, 823, 4935
Eco47I	GGWCC	6 cut(s)	1109, 1135, 1249, 3147, 3638, 3954
Eco47III	AGCGCT	1 cut(s)	4992
Eco53kI	GAGCTC	2 cut(s)	2135, 4239
Eco57I	CTGAAG	5 cut(s)	1065, 2173, 2355, 2850, 5015
Eco72I	CACGTG	1 cut(s)	4406
Eco81I	CCTNAGG	2 cut(s)	48, 3027
Eco88I	CYCGRG	2 cut(s)	1545, 5039
EcoICRI	GAGCTC	2 cut(s)	2135, 4239
EcoNI	CCTNNNNNAGG	4 cut(s)	469, 1545, 2497, 3479
EcoO109I	RGGNCCY	1 cut(s)	1553
EcoRI	GAATTC	1 cut(s)	3185
EcoRV	GATATC	3 cut(s)	218, 823, 4935
EcoT14I	CCWWGG	8 cut(s)	1058, 1927, 3449, 3957, 4059, 4554, 4685, 4745
EcoT22I	ATGCAT	1 cut(s)	135
EcoT38I	GRGCYC	2 cut(s)	2137, 4241
ErhI	CCWWGG	8 cut(s)	1058, 1927, 3449, 3957, 4059, 4554, 4685, 4745
FalI	AAGNNNNNCTT	4 cut(s)	2049, 2081, 4988, 5020
FaqI	GGGAC	6 cut(s)	453, 1499, 1885, 1921, 3502, 4277
FauNDI	CATATG	2 cut(s)	882, 1717
FbaI	TGATCA	2 cut(s)	1957, 3418
FblI	GTMKAC	2 cut(s)	699, 4127
FriOI	GRGCYC	2 cut(s)	2137, 4241
GlaI	GCGC	5 cut(s)	899, 3170, 4227, 4992, 5062
GsuI	CTGGAG	4 cut(s)	1347, 1624, 2370, 3930
HaeII	RGCGCY	3 cut(s)	901, 3172, 4994
HgaI	GACGC	2 cut(s)	2141, 3209
HhaI	GCGC	5 cut(s)	900, 3171, 4228, 4993, 5063
Hin1I	GRCGYC	1 cut(s)	2152
Hin6I	GCGC	5 cut(s)	898, 3169, 4226, 4991, 5061
HinP1I	GCGC	5 cut(s)	898, 3169, 4226, 4991, 5061
HincII	GTYRAC	3 cut(s)	2977, 3856, 4876
HindII	GTYRAC	3 cut(s)	2977, 3856, 4876
HindIII	AAGCTT	3 cut(s)	344, 2586, 2921
HphI	GGTGA	8 cut(s)	574, 584, 1177, 1854, 1985, 3062, 3820, 3854
HpyCH4IV	ACGT	4 cut(s)	442, 2299, 2638, 4405
HpySE526I	ACGT	4 cut(s)	442, 2299, 2638, 4405
Hsp92I	GRCGYC	1 cut(s)	2152
HspAI	GCGC	5 cut(s)	898, 3169, 4226, 4991, 5061
Kpn2I	TCCGGA	2 cut(s)	1815, 2200
Ksp22I	TGATCA	2 cut(s)	1957, 3418
LmnI	GCTCC	8 cut(s)	85, 412, 2132, 2356, 2874, 3451, 4750, 4777
MaeII	ACGT	4 cut(s)	442, 2299, 2638, 4405
MbiI	CCGCTC	1 cut(s)	4364
MfeI	CAATTG	4 cut(s)	356, 1782, 1904, 3422
MhlI	GDGCHC	5 cut(s)	309, 601, 2137, 3157, 4241
MlsI	TGGCCA	3 cut(s)	1284, 1902, 1926
MluNI	TGGCCA	3 cut(s)	1284, 1902, 1926
MlyI	GAGTC	9 cut(s)	1063, 1253, 1615, 1943, 2834, 2921, 4171, 4486, 4541
MmeI	TCCRAC	5 cut(s)	1227, 1469, 1598, 2772, 4647
Mox20I	TGGCCA	3 cut(s)	1284, 1902, 1926
Mph1103I	ATGCAT	1 cut(s)	135
MroI	TCCGGA	2 cut(s)	1815, 2200
MroXI	GAANNNNTTC	2 cut(s)	2587, 2856
MscI	TGGCCA	3 cut(s)	1284, 1902, 1926
MslI	CAYNNNNRTG	5 cut(s)	594, 1290, 3690, 3976, 4791
Msp20I	TGGCCA	3 cut(s)	1284, 1902, 1926
MspA1I	CMGCKG	4 cut(s)	146, 419, 801, 947
MunI	CAATTG	4 cut(s)	356, 1782, 1904, 3422
Mva1269I	GAATGC	7 cut(s)	1157, 1669, 1687, 2794, 3388, 3410, 4816
MvnI	CGCG	3 cut(s)	1428, 5051, 5061
NcoI	CCATGG	3 cut(s)	3957, 4059, 4554
NdeI	CATATG	2 cut(s)	882, 1717
NheI	GCTAGC	1 cut(s)	3740
NmuCI	GTSAC	7 cut(s)	1126, 1183, 1414, 2485, 3050, 3093, 3230
NruI	TCGCGA	1 cut(s)	1428
NsiI	ATGCAT	1 cut(s)	135
NspI	RCATGY	3 cut(s)	517, 1023, 3981
NspV	TTCGAA	1 cut(s)	2443
PaeR7I	CTCGAG	1 cut(s)	5039
PagI	TCATGA	4 cut(s)	1261, 1402, 3415, 4000
PciI	ACATGT	1 cut(s)	1019
PctI	GAATGC	7 cut(s)	1157, 1669, 1687, 2794, 3388, 3410, 4816
PdmI	GAANNNNTTC	2 cut(s)	2587, 2856
PflFI	GACNNNGTC	1 cut(s)	3952
PflMI	CCANNNNNTGG	1 cut(s)	2291
PinAI	ACCGGT	2 cut(s)	740, 1187
Ple19I	CGATCG	1 cut(s)	754
PleI	GAGTC	9 cut(s)	1062, 1252, 1614, 1942, 2833, 2920, 4170, 4486, 4541
PmaCI	CACGTG	1 cut(s)	4406
PmlI	CACGTG	1 cut(s)	4406
PpsI	GAGTC	9 cut(s)	1062, 1252, 1614, 1942, 2833, 2920, 4170, 4486, 4541
Ppu21I	YACGTR	3 cut(s)	2300, 2639, 4406
PscI	ACATGT	1 cut(s)	1019
PshAI	GACNNNNGTC	3 cut(s)	2190, 2911, 4161
PsiI	TTATAA	1 cut(s)	4346
Psp124BI	GAGCTC	2 cut(s)	2137, 4241
PspCI	CACGTG	1 cut(s)	4406
PstI	CTGCAG	2 cut(s)	946, 2683
PstNI	CAGNNNCTG	2 cut(s)	1088, 4415
PsyI	GACNNNGTC	1 cut(s)	3952
PvuI	CGATCG	1 cut(s)	754
PvuII	CAGCTG	4 cut(s)	146, 419, 801, 947
RruI	TCGCGA	1 cut(s)	1428
RseI	CAYNNNNRTG	5 cut(s)	594, 1290, 3690, 3976, 4791
SacI	GAGCTC	2 cut(s)	2137, 4241
SchI	GAGTC	9 cut(s)	1063, 1253, 1615, 1943, 2834, 2921, 4171, 4486, 4541
SduI	GDGCHC	5 cut(s)	309, 601, 2137, 3157, 4241
SfcI	CTRYAG	4 cut(s)	942, 1089, 2679, 3318
Sfr274I	CTCGAG	1 cut(s)	5039
SfuI	TTCGAA	1 cut(s)	2443
SinI	GGWCC	6 cut(s)	1109, 1135, 1249, 3147, 3638, 3954
SlaI	CTCGAG	1 cut(s)	5039
SmiI	ATTTAAAT	1 cut(s)	3117
SmiMI	CAYNNNNRTG	5 cut(s)	594, 1290, 3690, 3976, 4791
SmlI	CTYRAG	5 cut(s)	1047, 2997, 4240, 4391, 5039
SmoI	CTYRAG	5 cut(s)	1047, 2997, 4240, 4391, 5039
SnaBI	TACGTA	1 cut(s)	2300
SstI	GAGCTC	2 cut(s)	2137, 4241
StyI	CCWWGG	8 cut(s)	1058, 1927, 3449, 3957, 4059, 4554, 4685, 4745
SwaI	ATTTAAAT	1 cut(s)	3117
TaiI	ACGT	4 cut(s)	445, 2302, 2641, 4408
TatI	WGTACW	4 cut(s)	1306, 4695, 4707, 4729
TauI	GCSGC	4 cut(s)	1534, 2125, 4186, 4783
TseFI	GTSAC	7 cut(s)	1126, 1183, 1414, 2485, 3050, 3093, 3230
Tsp45I	GTSAC	7 cut(s)	1126, 1183, 1414, 2485, 3050, 3093, 3230
TspGWI	ACGGA	4 cut(s)	842, 924, 2327, 3873
Tth111I	GACNNNGTC	1 cut(s)	3952
Van91I	CCANNNNNTGG	1 cut(s)	2291
VneI	GTGCAC	2 cut(s)	305, 597
VpaK11BI	GGWCC	6 cut(s)	1109, 1135, 1249, 3147, 3638, 3954
XagI	CCTNNNNNAGG	4 cut(s)	469, 1545, 2497, 3479
XapI	RAATTY	8 cut(s)	1379, 1387, 2518, 3068, 3118, 3185, 3671, 4370
XbaI	TCTAGA	4 cut(s)	55, 1334, 2194, 2930
XceI	RCATGY	3 cut(s)	517, 1023, 3981
XcmI	CCANNNNNNNNNTGG	3 cut(s)	164, 853, 4683
XhoI	CTCGAG	1 cut(s)	5039
XmiI	GTMKAC	2 cut(s)	699, 4127
XmnI	GAANNNNTTC	2 cut(s)	2587, 2856
Zsp2I	ATGCAT	1 cut(s)	135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.