Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003731

Physical Location & Seq

Genomic Coordinates Forward (+)

12563936 .. 12577071

Gene Structure 13136 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1157000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5139 bp

ATGAAACCTTTGAGCTTTCTCAGTAAGGTTCATGACAAGAATCAACCTGAGGACCTAGAGACAACGCATATAAGTCATGCTAAGGAGCCTGATGCGGATATTGACCTGGGAGAAGTGTATTTTCTTATTATGCATTTTCTGTCTGCTGGACCGTGCCACAGAACTTGTGGACAGTTGTGGAATGAACTTCTTGAACATCAACTTTTACCTAGAAGATATCATGCTTGGTATTCAAGGAAGGGCATCCACAGTGGGGATGAAAATGATGATGGGAAATCATTTCCATTAAATTACAACATGCTAGTGCACAGGTATCCTCACATTGAAAATGATCATTTGGTAAAGCTTTTGAAGCAATTGATTTCAAGTGCAGCCCCACCATCGAGGGGTATGAGTGGTGGAAATGCTCCAAATGCAGCTGATGTGCCTACTCTCTTGGGACTTGGTTCATTTTCACTTCTGACCTATGAAAGGGATCAAGTGCACAATGAAATGAAGCGCCCTCCTGCTCATATGCGTTGGCCTCACATGAAATCTCATCAGGTTCAAGGGCTACGGTTGAGAGAAATTGGGGGAGGTTTCACCCGACATCATCGTGCACCGTCTATTCGTGCTGCATCCTATGCCATTGCAAAGCCCTCGACCATGTTTGAGAAGATGCAATTTACCAAGAGGCTAAGGGGTCATCGAAACTCTGTCTACTGTGCAACATTTGATTGCTCAGGGAGGTATGTGATTACTGGTTCAGATGATCGCTTAGTGAAGATCTGGTTAATGGAAACTGCATTTTGCTTGGCCAGCTGCCGTGGACATGAAGGAGACATCACGGATCTGGCTGTGAGTTCCAACAATGCTTTGGTGGCATCCTCCTCAAATGACTGTATGATTCGAGTATGGCGCTTGCCAGACGGGCTGCCAATTTCAGTATTGCGAGGACATACTGCAGCTGTTACTGCCATTACATTTAATCCCAGGCCAGGCTCTATGTACCACCTTCTATCTTCATCTGATGATGGAACTTGTAGGATATGGGATGCCAGGAGTTCTCAAGTGAGTCCAAAGATGTACATTCCAAGACCTTCAGATGCTGTAGTTGGGAGAAACAGTGGTCCATCTTCAAGCACTGTTACACAGGACCATCATATCTTCTGTTGTGCATTCAATGCTAATGGAACAGTCTTTGTCACCGGTAGCTCGGACACTCTTGCAAGGGTGTGGACTGCTTCTAAGCCAGGCTCAGATGAGCCAGACCAACCAAATCATGAGATTGATGTGTTATCTGGCCATGAGAATGATGTCAATTACGTACAATTTAGTGGTTGTGCTGTAGTATCTAGATTTATGGCAGCTGACCCCTCAAAGGAAGAGAACGCTCCCAAATTTAAAAATTCGTGGTTCAATCATGACAATATAGTCACTTGTTCTCGCGATGGGAGTGCAATTATCTGGATTCCCAGATCACGAAGATCACATGGAAAAGCTGGTCGTTGGACTCGGGCTTATCATTTGAAAGTTCCACCTCCACCAATGCCCCCTCAGCCTCCACGAGGAGGCCCTCGCCAGAGGATTCTTCCAACTCCTCGTGGTGTTAATATGATTACCTGGAGTCTTGACAATCGCTTTGTACTTGCTGCTATCATGGATTGCAGAATATGCGTGTGGAATGCTTCTGATGGTAGCTTGGTGCATTCACTGACAGGTCATACTGAATCTACATATGTGCTGGATGTTCATCCCTTCAACCCCAGGATAGCAATGAGTGCTGGCTATGACGGAAAAACAATTGTCTGGGATATATGGGAAGGCATACCTATTCGGATATTTGAAACTTCCCACTACAAATTGGTAGATGGAAAGTTCTCGCCGGATGGAACATCAATTGTCCTCTCTGATGATGTTGGCCAATTGTACATATTGGACAGTGGCCAAGGGGAGTCCCAGAATGATGCAAAATATGATCAGTTTTTTCTTGGTGATTATCGCCCACTCGTTCATGATAGCTTTGGAAATGTTCTTGACCAGGAAACTCAGCTTCCAGCATACCGGCGGAATATGCAAGATCTACTTTGCGATTCAGGCATGATACCGTATGAAGAACCCTACCAGAGTGCATACCAGAAGAGGCGGTTAGGAACTCTGGGTGCCGAGTGGCGTCCTTCCTCTTTAAAACTTGCTGTTGGGCCTGACTTCAGTCTAGATCCAGATTACCAAATTTTGCCTTTGGCAGACTTGGATATGTTGGCTGAACCTCCTCCAGAATTTTTAGACGCCATGGATTGGGAACCAGAAACTGAAATACGTAGTGATGATACGGATTCAGAGTACCATATTGCTGAAGATTATTCCACTGGAGGGGAGCAAGGAAGTTTAAGTTCAAATCCCTCTATTGATCCAGAGTGCAGTGAAGAAAGTGAGGATGAGGATGCTGAAATGGATGGACTTCGAAGATCAAAAAGGAAAAAACAAAGGGCTGACATTGAAGTCATGACTTCTTCCGGGAGGTGTGTCAAAAGAAGGAATTTGGATGAGTGTGCTGGCAATTCATTCAGAAGTAACAGAACAAGGAAATCAAGACATGGACGAAAAGCTTCAAGAAAGAAATGTTCCTCATCATTGAGACCTCAAAGAGCTGCTGCACGCAATGCTCTTAATCTATTTTCTAAGATTACTGGAAGATCTGCAGATGGAGAAGATGAAGATGGATTGGAAGATGATATGTCAGGAAGTGAATCCCCACTTCAGGATTCAAACATTGAGAGTGATGAATCTGATAAACAAAATCAGCAAGGCAAGCGTTTGAAAGGAAAAGAGGTTTTTCTGGATGAGTCTGAGGACATGGCTAAACCTATTGAACGCCCTGAGTTTCTAGTAAATACTGGGAGCAGTAGGAGGTTGGTGCTTAAATTGCCAAGACGGGAGTCAAATAAGCTCGTGTCTAGAGAAAGCACTGTACATAATTGTCGTAATGAGGATTTGGTTGATCAATCATATAGAGTGCCTCAAGAAGCAAATGAAAATGATATAAGTCCTCAGGATCCAATTTCTGTTCCGGGGGATAAAAAATGCAGCACATTTGAAACAGCAGTGAGTGGGCAATCACACAAAGTAGAAAATCATTTAGACTTCAAAGGGAGAATTAGTTGGGGAGGGTCCAGAGCACGAACAGCGAAGCGCCAGAGATCAGGAGAATTCATGTCATTGGATGCACTTGCCAGAGCCAGTGCAGTTGTTGGGCACAATGAAAAAGACTACACAAAACCTGAAAATATTTCTCCTCGTTCAGAAAGCCAACGGTACAGGGATAGGATGGACACAGCTGTAGTGAATGAGGAAACCACTGGTGCTAGTATCTCCGAAGGCTTTATTGGTGAAGCCAATGTTAAAGAGCATTCAAATTTTAGTGAATGCAAGGATCATGATCAATCGCCTAAATCTGTTTACATGGCTCCTTGGGATGCAAGTACTTCATCCTGCCTTGATAAGGATGGGACTGGTTTCTCTTTCGAACAAAATGAGAAGCTCATACCTGTCTCAACGAAATTAAGGTTGAGGAGGATTTCAAGGGATCCTAGTCCATGCAAAGAGGAAATTAACTCTGTGGTAGAGATGGAAAATGGTTGGACGATGGAACAGGGTCCAGTTGTGCCAGAGGTTAATGGAACCCATAAATTTATTCCGGATCATAGATATGATGGTTCACGAGAACCAGACAATCAATCTGATAAGAATGTTATTGGTGGCACACATGAATCTGCCGAATCCAGTTTACAAAACAACAAAATGTTCAGTGCTGTTTATAGAAGGGTGAAACCACATAGGGGTAGAAGTAATTCAGAAGGTGATAGTGGTGTTAAGGGAGAAGGTTGTTCGTATACTTCAAATACAAGCAACCACAATATCATTGCTGGAGACATTCAGGATGACACAACTGATGGAGGCCGCAGGACACGGTCCATGGGGTTGAAGGCGTCTGCATGTGATCCAAGTAGTCCAGATCATGATCCTAAGTTAGCGGAAGGACATGAGCCAGGTTATACATTTAGAATTAACCGGAAAAGTTCATTGGACAAGTTTCAACTTGGGAATGAAGAATGGGGATCAAGTTCAAGGACAACAGTTGGGTTGAGGTCTAGTAGAAACAGAAGAAGCAGTTATCTTGACATGAGCCCAATGGATAAAAGGAAGCCGCATCAGTCAGCAAAGAAAGTATCATGGTTAATGTTGTCAACACATGAGGAGAGCTCTAGATATATTCCCCAGATAGGGGACGAAGTTGTTTATCTGAGACAGGGGCATCAAGAGTATTTCGATCTAGGTGGATTGAGAGAAAACCCACCATGGAGGTTTATAAAGGGGAGAATAAGAGCTGTAGAATTTTGCAATGTTCAAGACCTAGAGTATTCTTCACTTGCAGGGTCTGGGGATAGCTGCTGTAAATTGACGCTTCAATTTGTAGATCCTTCTTCTGATGTGTATGGTAAATCTTTTAAAGTGACCCTGCCGGAAGTTACTGGTTTCCCAGACTTCATTGTTGAAAGAACACGCTATGACTCTTCCATAGAGAGAAATTGGTCATGTAGGGATCATTGCAAAGTCTGGTGGAAAAATGAGGGTGAGGATGACGGTAAGTGGTGGCATGGCAGGGTTACGTCTAAACGGTCTAAATCGCCCGAGTTCCCAGACAGTCCTTGGGAGATGTACACTGTTCAGTACAAGAGTGATCCTAGTGATACACAGCTGCATAGTCCTTGGGAGCTTTTTGACGAAAATACTCTATGGGAGCCTCCTCGCATTGATGATAAAAGCAAAAAGAAGCTGAATGCGGCCTTTTCCAAACTAGAGCGATCAGCCGGAAGTCGCCAGGATCCTTTTGGGGTTGACAAACTGATGCAACTTCAACAGAAGCCAAAGTTTACAAACTGGTGCGTGGTTCCGATATCTCTTGATGTGATCCAATCTAGGTTAGAGAACGACTACTATCGAAGCCTGGAAGCGCTGAAGCATGACTTTAAGGTTTTGCTGTTAAATGCTGAAACCTATCTCGAGAGCAATGCGGTCAAACGCGCATCAGATAAAGAGCTTTTAGGGAAATTGAAATGCCTATCAGACTGGTTCACAGAGACAATATTATCCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008360 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019221 GO:0019222 GO:0022603 GO:0022604 GO:0023052 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0034097 GO:0038111 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071345 GO:0071840 GO:0080090 GO:0098760 GO:0098761 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_resp Bromodomain WD40

Protein Analysis

1713

Amino Acids

192.81

Weight (kDa)

6.21

Isoelectric Point (pI)

53.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRWD1_N	PF25437	26 - 123	5.4e-34	BRWD1-like, N-terminal domain
Beta-prop_TEP1_2nd	PF25047	216 - 348	4.5e-13	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	217 - 304	1.4e-17	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	218 - 353	2.5e-27	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	218 - 351	2.5e-15	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	219 - 344	7.7e-08	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	220 - 351	4.3e-13	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	221 - 349	8.4e-22	WDR3 first beta-propeller domain
WDR55	PF24796	221 - 351	2.1e-07	WDR55
WD40_Prp19	PF24814	221 - 351	7.8e-16	Prp19 WD40 domain
WD40	PF00400	222 - 257	7.3e-08	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	223 - 300	2.1e-10	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	228 - 413	2.5e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_SCAP	PF24017	241 - 351	4.9e-09	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	264 - 324	4.1e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	264 - 354	1.9e-07	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	264 - 299	1.3e-07	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	274 - 355	9.1e-12	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	286 - 351	4.3e-09	THOC3 beta-propeller domain
WD40	PF00400	306 - 345	4.4e-07	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	528 - 645	3.4e-12	WDR5 beta-propeller domain
WD40_Prp19	PF24814	529 - 642	1.9e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	529 - 625	2e-06	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	529 - 653	4.8e-14	THOC3 beta-propeller domain
BRWD_AD	PF25313	1415 - 1589	2.9e-58	BRWD/PHIP ancillary domain-like domain
Bromodomain	PF00439	1631 - 1686	5.1e-07	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49430 AT5G49430
fragaria_vesca	FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490
malus_domestica	MD13G1156100.v1.1 MD16G1157000.v1.1
prunus_persica	Prupe.1G121900_v2.0.a1 Prupe.1G121900_v2.0.a1
pyrus_communis	pycom13g13310 pycom13g13320 pycom13g13330 pycom13g13340 pycom13g13350 pycom16g13190 pycom16g13200
rosa_chinensis	RchiOBHm_Chr4g0424601
rosa_laevigata	RLG00000007452
rosa_multiflora	Rmu_co8472673.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366520
rosa_rugosa	Rorug04G0198100 Rorug04G0198200 Rorug04G0198300
rosa_samantha	Rh4AG254900 Rh4BG260400 Rh4CG271900 Rh4DG255100
rosa_wichuraiana	Rw4G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4349
AasI	GACNNNNNNGTC	2 cut(s)	1412, 2480
AccB1I	GGYRCC	1 cut(s)	2141
AccB7I	CCANNNNNTGG	1 cut(s)	2277
AccI	GTMKAC	2 cut(s)	699, 3872
AccII	CGCG	2 cut(s)	1428, 5064
AccIII	TCCGGA	1 cut(s)	3676
AciI	CCGC	8 cut(s)	95, 2047, 2125, 3940, 4013, 4187, 4823, 5054
AcoI	YGGCCR	4 cut(s)	795, 1282, 1900, 1924
AcsI	RAATTY	9 cut(s)	1379, 1387, 2211, 2258, 2518, 3188, 3394, 3668, 4373
AcuI	CTGAAG	5 cut(s)	1065, 2173, 2355, 2723, 5018
AcyI	GRCGYC	3 cut(s)	2152, 2268, 3968
AfeI	AGCGCT	1 cut(s)	4995
AgeI	ACCGGT	1 cut(s)	1187
AjuI	GAANNNNNNNTTGG	4 cut(s)	3348, 3380, 4046, 4078
Alw21I	GWGCWC	5 cut(s)	309, 486, 601, 3160, 4244
Alw26I	GTCTC	7 cut(s)	53, 813, 2611, 3535, 3903, 4279, 5114
Alw44I	GTGCAC	3 cut(s)	305, 482, 597
AlwNI	CAGNNNCTG	3 cut(s)	1088, 2291, 4418
Ama87I	CYCGRG	3 cut(s)	1494, 4670, 5042
Aor13HI	TCCGGA	1 cut(s)	3676
Aor51HI	AGCGCT	1 cut(s)	4995
ApaLI	GTGCAC	3 cut(s)	305, 482, 597
ApoI	RAATTY	9 cut(s)	1379, 1387, 2211, 2258, 2518, 3188, 3394, 3668, 4373
ArsI	GACNNNNNNTTYG	2 cut(s)	1604, 1636
AsiGI	ACCGGT	1 cut(s)	1187
Asp700I	GAANNNNTTC	2 cut(s)	2587, 3829
AspLEI	GCGC	5 cut(s)	501, 900, 3174, 4996, 5066
AspS9I	GGNCC	9 cut(s)	52, 149, 1109, 1135, 1553, 2180, 3150, 3635, 3951
AsuC2I	CCSGG	2 cut(s)	2497, 3051
AsuHPI	GGTGA	7 cut(s)	574, 1177, 1985, 3380, 3817, 3851, 4625
AsuII	TTCGAA	2 cut(s)	2443, 3504
AvaI	CYCGRG	3 cut(s)	1494, 4670, 5042
AvaII	GGWCC	7 cut(s)	52, 149, 1109, 1135, 3150, 3635, 3951
AxyI	CCTNAGG	2 cut(s)	48, 3030
BaeGI	GKGCMC	4 cut(s)	309, 486, 601, 3237
BalI	TGGCCA	4 cut(s)	797, 1284, 1902, 1926
BamHI	GGATCC	3 cut(s)	3034, 3565, 4864
BanI	GGYRCC	1 cut(s)	2141
BanII	GRGCYC	2 cut(s)	4169, 4244
BauI	CACGAG	4 cut(s)	1545, 1581, 2930, 3699
Bbv12I	GWGCWC	5 cut(s)	309, 486, 601, 3160, 4244
BbvCI	CCTCAGC	1 cut(s)	1536
BceAI	ACGGC	1 cut(s)	789
BcgI	CGANNNNNNTGC	6 cut(s)	621, 655, 1418, 1452, 5033, 5067
BciVI	GTATCC	1 cut(s)	324
BclI	TGATCA	4 cut(s)	331, 1957, 2980, 3418
BcnI	CCSGG	2 cut(s)	2497, 3051
BcoDI	GTCTC	7 cut(s)	53, 813, 2611, 3535, 3903, 4279, 5114
BfmI	CTRYAG	6 cut(s)	942, 1089, 1326, 2679, 3318, 4368
BfoI	RGCGCY	4 cut(s)	502, 901, 3175, 4997
BfuI	GTATCC	1 cut(s)	324
BglI	GCCNNNNNGGC	1 cut(s)	910
BglII	AGATCT	3 cut(s)	765, 2059, 2675
BmcAI	AGTACT	1 cut(s)	3463
Bme18I	GGWCC	7 cut(s)	52, 149, 1109, 1135, 3150, 3635, 3951
BmeT110I	CYCGRG	3 cut(s)	1494, 4670, 5042
BmgT120I	GGNCC	9 cut(s)	52, 149, 1109, 1135, 1553, 2180, 3150, 3635, 3951
BmrI	ACTGGG	1 cut(s)	2886
BmuI	ACTGGG	1 cut(s)	2886
BoxI	GACNNNNGTC	2 cut(s)	2190, 2917
BplI	GAGNNNNNCTC	2 cut(s)	4226, 4258
BpmI	CTGGAG	4 cut(s)	1624, 2238, 2370, 3927
Bpu10I	CCTNAGC	4 cut(s)	81, 677, 721, 1536
Bpu14I	TTCGAA	2 cut(s)	2443, 3504
BpuEI	CTTGAG	2 cut(s)	1032, 2985
BpuMI	CCSGG	2 cut(s)	2497, 3051
BsaAI	YACGTR	2 cut(s)	1306, 2300
BsaBI	GATNNNNATC	3 cut(s)	1731, 3417, 3999
BsaHI	GRCGYC	3 cut(s)	2152, 2268, 3968
BsaI	GGTCTC	1 cut(s)	2611
BsaWI	WCCGGW	3 cut(s)	1187, 3676, 4050
BsaXI	ACNNNNNCTCC	4 cut(s)	1090, 1120, 3124, 3154
Bse118I	RCCGGY	2 cut(s)	1187, 2043
Bse21I	CCTNAGG	2 cut(s)	48, 3030
Bse3DI	GCAATG	7 cut(s)	627, 1761, 2647, 3900, 4387, 4585, 5056
Bse8I	GATNNNNATC	3 cut(s)	1731, 3417, 3999
BseAI	TCCGGA	1 cut(s)	3676
BseJI	GATNNNNATC	3 cut(s)	1731, 3417, 3999
BseMI	GCAATG	7 cut(s)	627, 1761, 2647, 3900, 4387, 4585, 5056
BseRI	GAGGAG	8 cut(s)	859, 1563, 1569, 2241, 3264, 3565, 4250, 4776
BseSI	GKGCMC	4 cut(s)	309, 486, 601, 3237
BsgI	GTGCAG	4 cut(s)	390, 2419, 2619, 3243
Bsh1236I	CGCG	2 cut(s)	1428, 5064
BshNI	GGYRCC	1 cut(s)	2141
BshTI	ACCGGT	1 cut(s)	1187
BsiHKAI	GWGCWC	5 cut(s)	309, 486, 601, 3160, 4244
BsiHKCI	CYCGRG	3 cut(s)	1494, 4670, 5042
BsiSI	CCGG	9 cut(s)	1188, 1865, 2044, 2496, 3050, 3677, 4051, 4502, 4851
BslFI	GGGAC	4 cut(s)	453, 1921, 3502, 4280
BsmAI	GTCTC	7 cut(s)	53, 813, 2611, 3535, 3903, 4279, 5114
BsmFI	GGGAC	4 cut(s)	453, 1921, 3502, 4280
BsmI	GAATGC	6 cut(s)	1157, 1669, 1687, 3388, 3410, 4825
Bso31I	GGTCTC	1 cut(s)	2611
BsoBI	CYCGRG	3 cut(s)	1494, 4670, 5042
Bsp119I	TTCGAA	2 cut(s)	2443, 3504
Bsp1286I	GDGCHC	7 cut(s)	309, 486, 601, 3160, 3237, 4169, 4244
Bsp13I	TCCGGA	1 cut(s)	3676
Bsp1407I	TGTACA	4 cut(s)	1065, 1908, 2950, 4698
Bsp19I	CCATGG	3 cut(s)	2271, 3954, 4337
Bsp68I	TCGCGA	1 cut(s)	1428
BspACI	CCGC	8 cut(s)	95, 2047, 2125, 3940, 4013, 4187, 4823, 5054
BspEI	TCCGGA	1 cut(s)	3676
BspFNI	CGCG	2 cut(s)	1428, 5064
BspHI	TCATGA	7 cut(s)	31, 1261, 1402, 1993, 2484, 3415, 3997
BspMAI	CTGCAG	2 cut(s)	946, 2683
BspT104I	TTCGAA	2 cut(s)	2443, 3504
BspT107I	GGYRCC	1 cut(s)	2141
BspTNI	GGTCTC	1 cut(s)	2611
BsrDI	GCAATG	7 cut(s)	627, 1761, 2647, 3900, 4387, 4585, 5056
BsrFI	RCCGGY	2 cut(s)	1187, 2043
BsrGI	TGTACA	4 cut(s)	1065, 1908, 2950, 4698
BssAI	RCCGGY	2 cut(s)	1187, 2043
BssNAI	GTATAC	1 cut(s)	3873
BssNI	GRCGYC	3 cut(s)	2152, 2268, 3968
BssSI	CACGAG	4 cut(s)	1545, 1581, 2930, 3699
BssT1I	CCWWGG	7 cut(s)	1927, 2271, 3449, 3954, 4337, 4688, 4748
Bst1107I	GTATAC	1 cut(s)	3873
Bst2BI	CACGAG	4 cut(s)	1545, 1581, 2930, 3699
Bst6I	CTCTTC	3 cut(s)	1359, 2114, 4558
BstACI	GRCGYC	3 cut(s)	2152, 2268, 3968
BstAPI	GCANNNNNTGC	5 cut(s)	623, 1163, 1653, 1760, 2642
BstAUI	TGTACA	4 cut(s)	1065, 1908, 2950, 4698
BstBAI	YACGTR	2 cut(s)	1306, 2300
BstBI	TTCGAA	2 cut(s)	2443, 3504
BstC8I	GCNNGC	6 cut(s)	799, 902, 1765, 2536, 2638, 2792
BstDSI	CCRYGG	4 cut(s)	805, 2271, 3954, 4337
BstENI	CCTNNNNNAGG	3 cut(s)	469, 1545, 3479
BstFNI	CGCG	2 cut(s)	1428, 5064
BstH2I	RGCGCY	4 cut(s)	502, 901, 3175, 4997
BstHHI	GCGC	5 cut(s)	501, 900, 3174, 4996, 5066
BstMAI	GTCTC	7 cut(s)	53, 813, 2611, 3535, 3903, 4279, 5114
BstNSI	RCATGY	2 cut(s)	301, 3978
BstPAI	GACNNNNGTC	2 cut(s)	2190, 2917
BstSFI	CTRYAG	6 cut(s)	942, 1089, 1326, 2679, 3318, 4368
BstSLI	GKGCMC	4 cut(s)	309, 486, 601, 3237
BstSNI	TACGTA	2 cut(s)	1306, 2300
BstUI	CGCG	2 cut(s)	1428, 5064
BstX2I	RGATCY	9 cut(s)	765, 829, 2059, 2197, 2675, 3034, 3565, 4456, 4864
BstYI	RGATCY	9 cut(s)	765, 829, 2059, 2197, 2675, 3034, 3565, 4456, 4864
BstZ17I	GTATAC	1 cut(s)	3873
Bsu36I	CCTNAGG	2 cut(s)	48, 3030
BsuI	GTATCC	1 cut(s)	324
BtgI	CCRYGG	4 cut(s)	805, 2271, 3954, 4337
BtgZI	GCGATG	1 cut(s)	1443
BtsI	GCAGTG	2 cut(s)	2407, 3090
BtuMI	TCGCGA	1 cut(s)	1428
Cac8I	GCNNGC	6 cut(s)	799, 902, 1765, 2536, 2638, 2792
CaiI	CAGNNNCTG	3 cut(s)	1088, 2291, 4418
CciI	TCATGA	7 cut(s)	31, 1261, 1402, 1993, 2484, 3415, 3997
CfoI	GCGC	5 cut(s)	501, 900, 3174, 4996, 5066
Cfr10I	RCCGGY	2 cut(s)	1187, 2043
Cfr13I	GGNCC	9 cut(s)	52, 149, 1109, 1135, 1553, 2180, 3150, 3635, 3951
CseI	GACGC	4 cut(s)	2141, 2276, 3957, 4450
CspAI	ACCGGT	1 cut(s)	1187
CspCI	CAANNNNNGTGG	2 cut(s)	146, 181
DraI	TTTAAA	3 cut(s)	1384, 2166, 4489
DrdI	GACNNNNNNGTC	2 cut(s)	1412, 2480
DseDI	GACNNNNNNGTC	2 cut(s)	1412, 2480
EaeI	YGGCCR	4 cut(s)	795, 1282, 1900, 1924
Eam1104I	CTCTTC	3 cut(s)	1359, 2114, 4558
EarI	CTCTTC	3 cut(s)	1359, 2114, 4558
EciI	GGCGGA	1 cut(s)	2062
Ecl136II	GAGCTC	1 cut(s)	4242
Eco105I	TACGTA	2 cut(s)	1306, 2300
Eco130I	CCWWGG	7 cut(s)	1927, 2271, 3449, 3954, 4337, 4688, 4748
Eco24I	GRGCYC	2 cut(s)	4169, 4244
Eco31I	GGTCTC	1 cut(s)	2611
Eco32I	GATATC	2 cut(s)	218, 4938
Eco47I	GGWCC	7 cut(s)	52, 149, 1109, 1135, 3150, 3635, 3951
Eco47III	AGCGCT	1 cut(s)	4995
Eco53kI	GAGCTC	1 cut(s)	4242
Eco57I	CTGAAG	5 cut(s)	1065, 2173, 2355, 2723, 5018
Eco81I	CCTNAGG	2 cut(s)	48, 3030
Eco88I	CYCGRG	3 cut(s)	1494, 4670, 5042
EcoICRI	GAGCTC	1 cut(s)	4242
EcoNI	CCTNNNNNAGG	3 cut(s)	469, 1545, 3479
EcoO109I	RGGNCCY	2 cut(s)	52, 1553
EcoRI	GAATTC	1 cut(s)	3188
EcoRV	GATATC	2 cut(s)	218, 4938
EcoT14I	CCWWGG	7 cut(s)	1927, 2271, 3449, 3954, 4337, 4688, 4748
EcoT22I	ATGCAT	1 cut(s)	135
EcoT38I	GRGCYC	2 cut(s)	4169, 4244
ErhI	CCWWGG	7 cut(s)	1927, 2271, 3449, 3954, 4337, 4688, 4748
FalI	AAGNNNNNCTT	4 cut(s)	2049, 2081, 4991, 5023
FaqI	GGGAC	4 cut(s)	453, 1921, 3502, 4280
FauNDI	CATATG	2 cut(s)	513, 1717
FbaI	TGATCA	4 cut(s)	331, 1957, 2980, 3418
FblI	GTMKAC	2 cut(s)	699, 3872
FriOI	GRGCYC	2 cut(s)	4169, 4244
GlaI	GCGC	5 cut(s)	500, 899, 3173, 4995, 5065
GsuI	CTGGAG	4 cut(s)	1624, 2238, 2370, 3927
HaeII	RGCGCY	4 cut(s)	502, 901, 3175, 4997
HapII	CCGG	9 cut(s)	1188, 1865, 2044, 2496, 3050, 3677, 4051, 4502, 4851
HgaI	GACGC	4 cut(s)	2141, 2276, 3957, 4450
HhaI	GCGC	5 cut(s)	501, 900, 3174, 4996, 5066
Hin1I	GRCGYC	3 cut(s)	2152, 2268, 3968
Hin6I	GCGC	5 cut(s)	499, 898, 3172, 4994, 5064
HinP1I	GCGC	5 cut(s)	499, 898, 3172, 4994, 5064
HincII	GTYRAC	2 cut(s)	4227, 4879
HindII	GTYRAC	2 cut(s)	4227, 4879
HindIII	AAGCTT	2 cut(s)	344, 2586
HpaII	CCGG	9 cut(s)	1188, 1865, 2044, 2496, 3050, 3677, 4051, 4502, 4851
HphI	GGTGA	7 cut(s)	574, 1177, 1985, 3380, 3817, 3851, 4625
HpyCH4IV	ACGT	3 cut(s)	1305, 2299, 4649
HpySE526I	ACGT	3 cut(s)	1305, 2299, 4649
Hsp92I	GRCGYC	3 cut(s)	2152, 2268, 3968
HspAI	GCGC	5 cut(s)	499, 898, 3172, 4994, 5064
Kpn2I	TCCGGA	1 cut(s)	3676
Ksp22I	TGATCA	4 cut(s)	331, 1957, 2980, 3418
LmnI	GCTCC	8 cut(s)	85, 412, 1378, 2356, 2880, 3451, 4753, 4780
MaeII	ACGT	3 cut(s)	1305, 2299, 4649
MaeIII	GTNAC	8 cut(s)	949, 1126, 1183, 1414, 2552, 4492, 4507, 4645
MfeI	CAATTG	4 cut(s)	356, 1782, 1878, 1904
MflI	RGATCY	9 cut(s)	765, 829, 2059, 2197, 2675, 3034, 3565, 4456, 4864
MhlI	GDGCHC	7 cut(s)	309, 486, 601, 3160, 3237, 4169, 4244
MlsI	TGGCCA	4 cut(s)	797, 1284, 1902, 1926
MluNI	TGGCCA	4 cut(s)	797, 1284, 1902, 1926
MlyI	GAGTC	7 cut(s)	1063, 1486, 1615, 1943, 2834, 2927, 4544
MmeI	TCCRAC	4 cut(s)	870, 1469, 1598, 3599
Mox20I	TGGCCA	4 cut(s)	797, 1284, 1902, 1926
Mph1103I	ATGCAT	1 cut(s)	135
MroI	TCCGGA	1 cut(s)	3676
MroXI	GAANNNNTTC	2 cut(s)	2587, 3829
MscI	TGGCCA	4 cut(s)	797, 1284, 1902, 1926
MslI	CAYNNNNRTG	4 cut(s)	302, 594, 1290, 3687
Msp20I	TGGCCA	4 cut(s)	797, 1284, 1902, 1926
MspA1I	CMGCKG	6 cut(s)	419, 801, 947, 1349, 3317, 4738
MspI	CCGG	9 cut(s)	1188, 1865, 2044, 2496, 3050, 3677, 4051, 4502, 4851
MunI	CAATTG	4 cut(s)	356, 1782, 1878, 1904
Mva1269I	GAATGC	6 cut(s)	1157, 1669, 1687, 3388, 3410, 4825
MvnI	CGCG	2 cut(s)	1428, 5064
NciI	CCSGG	2 cut(s)	2497, 3051
NcoI	CCATGG	3 cut(s)	2271, 3954, 4337
NdeI	CATATG	2 cut(s)	513, 1717
NmeAIII	GCCGAG	1 cut(s)	2170
NmuCI	GTSAC	3 cut(s)	1183, 1414, 4492
NruI	TCGCGA	1 cut(s)	1428
NsiI	ATGCAT	1 cut(s)	135
NspI	RCATGY	2 cut(s)	301, 3978
NspV	TTCGAA	2 cut(s)	2443, 3504
PaeR7I	CTCGAG	1 cut(s)	5042
PagI	TCATGA	7 cut(s)	31, 1261, 1402, 1993, 2484, 3415, 3997
PctI	GAATGC	6 cut(s)	1157, 1669, 1687, 3388, 3410, 4825
PdmI	GAANNNNTTC	2 cut(s)	2587, 3829
PflFI	GACNNNGTC	1 cut(s)	3949
PflMI	CCANNNNNTGG	1 cut(s)	2277
PfoI	TCCNGGA	1 cut(s)	2495
PinAI	ACCGGT	1 cut(s)	1187
PleI	GAGTC	7 cut(s)	1062, 1486, 1614, 1942, 2833, 2926, 4544
PpsI	GAGTC	7 cut(s)	1062, 1486, 1614, 1942, 2833, 2926, 4544
Ppu21I	YACGTR	2 cut(s)	1306, 2300
PpuMI	RGGWCCY	1 cut(s)	52
PshAI	GACNNNNGTC	2 cut(s)	2190, 2917
PsiI	TTATAA	1 cut(s)	4349
Psp124BI	GAGCTC	1 cut(s)	4244
Psp5II	RGGWCCY	1 cut(s)	52
PspPI	GGNCC	9 cut(s)	52, 149, 1109, 1135, 1553, 2180, 3150, 3635, 3951
PspPPI	RGGWCCY	1 cut(s)	52
PstI	CTGCAG	2 cut(s)	946, 2683
PstNI	CAGNNNCTG	3 cut(s)	1088, 2291, 4418
PsuI	RGATCY	9 cut(s)	765, 829, 2059, 2197, 2675, 3034, 3565, 4456, 4864
PsyI	GACNNNGTC	1 cut(s)	3949
PvuII	CAGCTG	6 cut(s)	419, 801, 947, 1349, 3317, 4738
RruI	TCGCGA	1 cut(s)	1428
RseI	CAYNNNNRTG	4 cut(s)	302, 594, 1290, 3687
SacI	GAGCTC	1 cut(s)	4244
Sau96I	GGNCC	9 cut(s)	52, 149, 1109, 1135, 1553, 2180, 3150, 3635, 3951
ScaI	AGTACT	1 cut(s)	3463
SchI	GAGTC	7 cut(s)	1063, 1486, 1615, 1943, 2834, 2927, 4544
SduI	GDGCHC	7 cut(s)	309, 486, 601, 3160, 3237, 4169, 4244
SfcI	CTRYAG	6 cut(s)	942, 1089, 1326, 2679, 3318, 4368
Sfr274I	CTCGAG	1 cut(s)	5042
SfuI	TTCGAA	2 cut(s)	2443, 3504
SinI	GGWCC	7 cut(s)	52, 149, 1109, 1135, 3150, 3635, 3951
SlaI	CTCGAG	1 cut(s)	5042
SmiMI	CAYNNNNRTG	4 cut(s)	302, 594, 1290, 3687
SmlI	CTYRAG	3 cut(s)	1047, 3000, 5042
SmoI	CTYRAG	3 cut(s)	1047, 3000, 5042
SnaBI	TACGTA	2 cut(s)	1306, 2300
SsiI	CCGC	8 cut(s)	95, 2047, 2125, 3940, 4013, 4187, 4823, 5054
SspI	AATATT	2 cut(s)	3268, 5127
SstI	GAGCTC	1 cut(s)	4244
StyI	CCWWGG	7 cut(s)	1927, 2271, 3449, 3954, 4337, 4688, 4748
TaiI	ACGT	3 cut(s)	1308, 2302, 4652
TaqI	TCGA	9 cut(s)	383, 641, 688, 889, 2443, 3504, 4308, 4981, 5043
TatI	WGTACW	7 cut(s)	1065, 1624, 1908, 2950, 3461, 4698, 4710
TauI	GCSGC	3 cut(s)	3942, 4189, 4826
TseFI	GTSAC	3 cut(s)	1183, 1414, 4492
Tsp45I	GTSAC	3 cut(s)	1183, 1414, 4492
TspGWI	ACGGA	3 cut(s)	842, 1788, 2327
Tth111I	GACNNNGTC	1 cut(s)	3949
Van91I	CCANNNNNTGG	1 cut(s)	2277
VneI	GTGCAC	3 cut(s)	305, 482, 597
VpaK11BI	GGWCC	7 cut(s)	52, 149, 1109, 1135, 3150, 3635, 3951
XagI	CCTNNNNNAGG	3 cut(s)	469, 1545, 3479
XapI	RAATTY	9 cut(s)	1379, 1387, 2211, 2258, 2518, 3188, 3394, 3668, 4373
XbaI	TCTAGA	4 cut(s)	1334, 2194, 2936, 4244
XceI	RCATGY	2 cut(s)	301, 3978
XcmI	CCANNNNNNNNNTGG	4 cut(s)	164, 853, 4686, 4868
XhoI	CTCGAG	1 cut(s)	5042
XmiI	GTMKAC	2 cut(s)	699, 3872
XmnI	GAANNNNTTC	2 cut(s)	2587, 3829
ZrmI	AGTACT	1 cut(s)	3463
Zsp2I	ATGCAT	1 cut(s)	135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.