Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003731

Physical Location & Seq

Genomic Coordinates Forward (+)

16970350 .. 16988317

Gene Structure 17968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007452

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5379 bp

ATGCCCTGCATAGCTTTTATTATGGACAAGCAGGTTTTAGACAACCTTGTTGTAGTCAAGGAAAATGAGCTCTTTCTGGATCCCCACATCAAATCCTCAAACGCTAAAGCATGGCTTCTTAAAGGTTTTACAGGAGCTATGGCTCTTCAAAAGTTTCCTTGTGGTGATGCACCTTCTGTTAGTATGAAACCCTTGAGCTTTCTCAGTAAGGTGCATGACAGCACTCTACATGAGGATGTAGAGACAAATCATGCCAGGGAACAAGATGTGGATGTTGACCTCAGAGAAGTCTATTTCCTCATTATGCATTTTCTTTCAGCTGGACCATGTCATAAAACTTGTGTACAGTTCTGGAACGAACTTCTTGAACATCAACTTTTACCTAGAAGATATCACGCATGGTATTCAAGGAAAGGCATGCATAGTGGGGATGAAAATGATGATGGGCAATCATTCCCCTTAAATTACAATATGTTGTTGGCAAGGTATCCTCACATTGAGCAGGATCACTTGGTAAAGCTTTTGAAGCAAATGATTTTGAGTACAGCTCCACCATCAAGGGGAATGAGTGGTGGAAATGCCCCTAATGCAGCTGATGTACCAACTCTCTTGGGAACGGGCTCATTTTCACTTCTGAGCTATGATAGGGATCAAGAAAACAATGAAATGAACAGGCCTGCTGCTTACATGCGTTGGCCTCACATCAAAGCTAATCAGGTGCATGGGCTAGGTTTGAGGGAAATTGGGGGAGGTTTCACTAGACATCACCGTGCACCATCTGTTCGTGCTGCATCCTATGCCATTGCGAAACCATCAACCATGTTTCAGAAGATGAAAAATACCAAGAGAATAAGGGGCCATCGAAACGCTGTCTATTGTGCAATATTTGATCGCTCAGGTAGATATGTTATCACTGGTTCAGATGATCGCCTAGTGAAGATCTGGTTAATAGAAACTGCATTTTGCTTGGCCAGCTGCCGTGGACATGAAGGTGACATCACTGATTTAGCTGTGAGCTCCAACAATGCTTTGGTGGCATCGGCTTCAAATGACTGTATCATACGAGTTTGGCGCTTGCCAGATGGGCTTCCAATTTCAGTGTTGCGGGGACACACCGGAGCTGTGACTGCAATTGCATTTAGTCCCAGGCCTGGCTCTATATACCACCTTCTATCTTCCTCTGATGATGGAACTTGTAGGATATGGGATGCCAGGAATTCTCAAGTGACTCCGAGGATATACATTCCAAAACCTTCAGATACTATAACTGGGAGAAACAGTGGTCCATCCTCGAGCACTGTACAGAGCCATCAAATCTTCTGTTGTGCATTTAATGCTAATGGAACAGTCTTTGTTACCGGTAGCTCAGACACTCTTGCAAGGGTGTGGATTGCTTCCAAGCCAGGCTCAGATGAGTCGGAACAACCGAATCATGAAATAGATGTGTTATCTGGCCATGAGAATGATGTCAATTACGTACAATTTAGTGGTTGTGCTGTAGTATCTAGATTTATGGCAGCTGACACCTCAAAGGAAGAGAATGTTCCAAAATTTAAAAATTCATGGTTCAATCATGACAATATAGTTACTTGCTCTCGCGATGGGAGTGCAATTATCTGGATTCCAAAGTCACGAAGATCACATGGAAAAGCTGGTCGTTGGACACGAGCTTATCATCTGAAAGTTCCACCTCCACCAATGCCTCCTCAACCTTCACGTGGAGGCCCCCGCCAGAGAATTCTTCCAACTCCTCGTGGTGTTAATATGATTACCTGGAGTCTTGATAATCGCTTTGTTCTTGCTGCTATCATGGATTGCAGAATATGTGTATGGAATGCATCTGATGGTAGCTTGGTCCATTCTTTAACTGGTCACAGTGATTCTACATATGTTCTGGATGTTCACCCTTTCAACCCCAGGTTGGCAATGAGTGCTGGCTATGATGGAAAAACAATTGTCTGGGATATATGGGAAGGCATGCCTATCCGGATATATCAAACTTCACAGTTCAAATTGGTTGACGGAAAGTTTTCACCGGACGGAACGTCAATTATACTATCTGATGATGTTGGCCAACTCTACATATTGGACAGTGGCCAAGGGGAGTCCCACAATGATGCAAAATATGATCAGTTCTTTCTTGGTGATTATCGCCCAATCATTCAAGATAGCTTCGGAAATGTTCTTGACCAGGAAACTCAGATTCCAGCATACCGACGAAATATGCAGGATCTACTTTGTGATTCAGGCATGATACCATATCCGGAGCCCTACCAGAGTACATACCAGAAGAGACGGCTAGGAGCTCTGGGTTCTGAGTGGCGTCCTTCCTCTTTAAAACTTGCTGTTGGGCCTGACTTCAGTCTAGATCCAGATTTCCAAATGCTACCTTTGGCAGACTTGGATATGTTATTGACCGAGCCACTACCAGAGTTTGTAGATGCCATGGATTGGGAACCAGAAAATGAAAGGCAAAGTGATGATGCTGATTCAGAGTACAATATTACTGAAGATTATTCTACTGGAGGGGAGCAAGGAAGTTTAAGTTCAAACCCTTCTGTTGATCCAGACTGCAGTGCAGAAGATAGTGACGAGGATGCTCAACTGGATGGACTACGAAGATCACGAAGGAAAAAACAAAAGGCTGATATTGAAGTCATGACGTCTTCTGGAAGGTGTGTTAAAAGGAGGAACTTGGATGAGCGTGATGATCACTCTTTCAGAAGTAATAGAACAAGGAAATCAAGGCATGGCCGAAAAGCTTCAAAAAAGAAATGCTCCGCATCAAAGTCACTGAGACCTCGAAGAGTTGCTGCACGCAATGCCCTTACACTATTTTCTAAGATAACTGGACGAGATACTGATGGAGAAGATGAAGATGGATCCGAAGGAGAAATTTCAGGTAGTGAATCCACGCTGCAAGATTCAAATATTGAGAGTGACGGATCCGATAGGGAAAATCACCAAAGCAACTATATGAAAGGAAAACAGGTTTTACTCGATGAATCCGAGGATCTGGTCAAATCTCATGAACTTCATGAATTTCAAAGAAACTCTGGGAACAGGAGGAGATTAGTGCTAAAACTGCCAAGACGGCCGTCAAATACAAATACACTTGTGGATACAGAAAGCACACTACATAAGTGTGGAAATCCGGCTTATTTGGGTGACCAATCTTGTAGTCACAAAGCCAGTGAAGCAAATGAGACTCATATGAGCTCTCAGGATCTGAGGAGCTCTCCTGGTCATAAAAATAGCAGCATGTTTGAAAGAGCAGTGGGAGGGCAATCAAACAAACTTGAAAATTATTTAGACTTGACTGAGAATTACAAAGTTGGAAGTATAAGATGGGGAGGGTCCAGAGCTCGAACATTGAAGCGGCTGAGAGTGGGAGAAGCCACATCACTGGATGCACTTGCTACTACCAGTGTAGCTGTTGTTGGTCACACTGAAAAAAATTACACAGAATCTGAAAAAGATTGTCGAAAAATGTCCCCTCAATTAGAAAGCCAAAGGAATGGGGATATGATGGATGGAGTGATTATGACAAATGAGAGAACTATTGGGACTAGTACCTCTGATGGTCTTAATGGTAAAGCCGAGGTTAATGAGCACTCAGGATTTAGTGAATGCAAGGATAATGACCAATCACCTAAATCTGTTCACATGGCTTCTTGGGATGCAAGCACTGCCTCTTACCTCGACAAGGATGGAAGTGTTTTCTCTTCTGAACAAAACGAGAAGCTCACACCAGTCTCAACAAAATTAAGGTTGAGGAAGATTTCGAGGGGCCCTGAAAGTCCTTGCAAACAGGAAATGAGCCCTGTAGTAGAGAACTTGGAAAGCGGTAGACGTAACACATTGCCTGAAAGCCTTTCAACAATAGATCATGATTCAATTGTGCCGGAGGATAATGGCACGCATAAATTTGATTCAGATCAAAGATGCAGCGGCTTACGAGAATCAGACACTCAAATTGAATCAGTCGAGTCCCATCTAAATAGAAACAAAATGTTTAGTGCTGTTTATAGGAGGGTGAAATCACATAGAGGGCAGACTAATATAGAAGGTGAAAGTAGTAGGAAGGGAGAAGGATCTTCACAAATTTCCAACACTATTGACCAAAATCTCATTGCTGCAGTGGACTGCCATGACGATTTAATTGATGGAGCTCGTAGGACACGGTCCATGGGGTTGAAGGCATCTACCCATGATGTGGATCATGATCTCAAGTTAAGTCAAGGCCATGAACCTAGGTATACATTCAGAAGTGCCCAGAACAACACTGTGCACAAATGTCAATTTCAGAATGAAGAACAGGGATCAAGTTCAAGGACAGCAGTGGGGTTGAGATCTACTAGAAACAGGAGTTATTATGATCATGACATGCATCCAATAGATAAAAGGAAGTCGCATCAATCAATGAGGAAAGGAATATGGTTGATGTTGTCGGCACATGAGGAGAGCTCACGATATATTCCCCAGTTGGGGGATGAAGTTGTCTATTTGAGACAGGGGCATCAGGAGTATTTTGATCTTGGTAGGTTGAGAGAAGACCCACCTTGGGCTTTTATAAAGCAAAGAATAAGAGCTGTAGAATTTTGTAAAGTTCAAGGCCTAGAGTATTCTTTACTTCCAGGATCTGGGGATAGCTGCTGTAAATTGACCCTTGAATTTGTAGATCCTTCATCGGATGTTTACTGCAAATCTTTCAAAATGACTCTGCCTGAAGTTACTGGTTTCCCCGATTTCATTGTTGAAAGAACTCGCTATGTTGCTGCAATTGAGAAAAAATGGTCATGTAGGGATAAGTGCAAAGTCTGGTGGAAAAATGACGGTGACGATGATGGTAGTTGGTGGGATGGCCGGGTTGTGCTTATTCAGCCCAAATCTCCCGAATTTCCAGACAGTCCATGGGAAACGTGTACGGTTCTGTACAAGACTGTTCCTAAAGAGACACAGCTACATAGTCCTTGGGAGCTTTTTGATACCGATACTCAATGGGTTCAGCCTCAGATTGATGATAAGAGCAAAAACAAGCTGCATTCTGCCTTTGCCAAACTAGAGAAATTAGCCAGCAGTCGTCAGGATTCGTTAGGGATTAATAAATTGAAGCAGCTTCAGGAGAAGCCGAAATTCACAAATTGGTGTGCAGTTCCAATATCTCTTGAGTTGATCCAGAGTAGGTTAGAAAACGACTATTATCGAAGTTTGGAAGCACTGAAGCATGACTTTAAGGTAATGTTGTTAGATGCGCAAGCCTATGTAGAAAGCCATGTGGAGTCTTCAGGCAAAACGAAACGGACAGCAGATAAAGAGTTTCTAGAGAAACTGAAATGCCTATCAGGCTGGTTCACAGAGACAATTTCGTCCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008360 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019221 GO:0019222 GO:0022603 GO:0022604 GO:0023052 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0034097 GO:0038111 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071345 GO:0071840 GO:0080090 GO:0098760 GO:0098761 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_resp Bromodomain WD40

Protein Analysis

1793

Amino Acids

201.58

Weight (kDa)

6.42

Isoelectric Point (pI)

50.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRWD1_N	PF25437	82 - 182	4.6e-34	BRWD1-like, N-terminal domain
Beta-prop_TEP1_2nd	PF25047	274 - 409	3.2e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	275 - 362	1.1e-17	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	276 - 410	3.5e-15	CDC20/Fizzy WD40 domain
WDR55	PF24796	276 - 409	6.8e-07	WDR55
Beta-prop_WDR3_1st	PF25173	277 - 408	1.6e-22	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	278 - 360	1.1e-10	Prp19 WD40 domain
WD40_Gbeta	PF25391	278 - 409	1.7e-13	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	278 - 410	2.1e-26	WDR5 beta-propeller domain
WD40	PF00400	280 - 315	7.5e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	282 - 358	8.9e-10	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	287 - 402	8.8e-07	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	288 - 496	4.9e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_SCAP	PF24017	299 - 404	2.7e-09	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	320 - 383	1.5e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	322 - 357	1.6e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	322 - 414	3.5e-08	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	327 - 413	5.8e-06	EIF3I
Beta-prop_THOC3	PF25174	329 - 412	4.2e-12	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	332 - 421	2.9e-12	WDHD1 first WD40 domain
WD40_Prp19	PF24814	354 - 415	6.1e-06	Prp19 WD40 domain
WD40	PF00400	364 - 403	4.6e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	585 - 701	4.3e-11	WDR5 beta-propeller domain
WD40_Prp19	PF24814	586 - 698	2.7e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	586 - 663	2.1e-06	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	587 - 710	3.9e-14	THOC3 beta-propeller domain
BRWD_AD	PF25313	1490 - 1666	1.1e-55	BRWD/PHIP ancillary domain-like domain
Bromodomain	PF00439	1707 - 1757	1.8e-06	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0003731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49430 AT5G49430
fragaria_vesca	FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490
malus_domestica	MD13G1156100.v1.1 MD16G1157000.v1.1
prunus_persica	Prupe.1G121900_v2.0.a1 Prupe.1G121900_v2.0.a1
pyrus_communis	pycom13g13310 pycom13g13320 pycom13g13330 pycom13g13340 pycom13g13350 pycom16g13190 pycom16g13200
rosa_chinensis	RchiOBHm_Chr4g0424601
rosa_laevigata	RLG00000007452
rosa_multiflora	Rmu_co8472673.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366520
rosa_rugosa	Rorug04G0198100 Rorug04G0198200 Rorug04G0198300
rosa_samantha	Rh4AG254900 Rh4BG260400 Rh4CG271900 Rh4DG255100
rosa_wichuraiana	Rw4G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4574
AasI	GACNNNNNNGTC	1 cut(s)	5368
AatII	GACGTC	1 cut(s)	2666
Acc16I	TGCGCA	1 cut(s)	5256
Acc36I	ACCTGC	1 cut(s)	22
AccB7I	CCANNNNNTGG	3 cut(s)	2451, 4216, 4643
AccI	GTMKAC	2 cut(s)	3850, 4259
AccII	CGCG	1 cut(s)	1599
AccIII	TCCGGA	2 cut(s)	1986, 2263
AciI	CCGC	6 cut(s)	1105, 1729, 2781, 3379, 3846, 3951
AcoI	YGGCCR	7 cut(s)	969, 1453, 2071, 2095, 2752, 3095, 4864
AcuI	CTGAAG	7 cut(s)	1239, 2344, 2529, 4749, 5105, 5243, 5271
AcvI	CACGTG	1 cut(s)	1718
AcyI	GRCGYC	2 cut(s)	2323, 2663
AflIII	ACRYGT	1 cut(s)	4922
AgeI	ACCGGT	1 cut(s)	1358
AhdI	GACNNNNNGTC	1 cut(s)	2044
AhlI	ACTAGT	1 cut(s)	3569
Alw26I	GTCTC	8 cut(s)	236, 2287, 2791, 3200, 3760, 4504, 4949, 5354
Alw44I	GTGCAC	2 cut(s)	771, 4289
AlwNI	CAGNNNCTG	3 cut(s)	3229, 3470, 4643
Ama87I	CYCGRG	1 cut(s)	1291
Aor13HI	TCCGGA	2 cut(s)	1986, 2263
ApaI	GGGCCC	1 cut(s)	3794
ApaLI	GTGCAC	2 cut(s)	771, 4289
AseI	ATTAAT	1 cut(s)	5103
AsiGI	ACCGGT	1 cut(s)	1358
Asp700I	GAANNNNTTC	2 cut(s)	2029, 2761
AspA2I	CCTAGG	1 cut(s)	4253
AspLEI	GCGC	2 cut(s)	1074, 5257
AsuC2I	CCSGG	1 cut(s)	4868
AvaI	CYCGRG	1 cut(s)	1291
AvaII	GGWCC	5 cut(s)	323, 1283, 1855, 3357, 4185
AvrII	CCTAGG	1 cut(s)	4253
BaeGI	GKGCMC	4 cut(s)	775, 3794, 4276, 4293
BalI	TGGCCA	4 cut(s)	971, 1455, 2073, 2097
BamHI	GGATCC	3 cut(s)	79, 2882, 2945
BauI	CACGAG	2 cut(s)	1665, 1752
BbrPI	CACGTG	1 cut(s)	1718
BbsI	GAAGAC	3 cut(s)	2658, 4560, 5277
BceAI	ACGGC	4 cut(s)	963, 2312, 3082, 3110
BcgI	CGANNNNNNTGC	2 cut(s)	1589, 1623
BciVI	GTATCC	2 cut(s)	498, 3115
BclI	TGATCA	3 cut(s)	2128, 2710, 4378
BcnI	CCSGG	1 cut(s)	4868
BcoDI	GTCTC	8 cut(s)	236, 2287, 2791, 3200, 3760, 4504, 4949, 5354
BcuI	ACTAGT	1 cut(s)	3569
BfmI	CTRYAG	6 cut(s)	1497, 2572, 3825, 4137, 4593, 5375
BfoI	RGCGCY	1 cut(s)	1075
BfuAI	ACCTGC	1 cut(s)	22
BfuI	GTATCC	2 cut(s)	498, 3115
BglI	GCCNNNNNGGC	3 cut(s)	1084, 3094, 5346
BglII	AGATCT	2 cut(s)	939, 4352
BlnI	CCTAGG	1 cut(s)	4253
Bme18I	GGWCC	5 cut(s)	323, 1283, 1855, 3357, 4185
BmeRI	GACNNNNNGTC	1 cut(s)	2044
BmeT110I	CYCGRG	1 cut(s)	1291
BmrI	ACTGGG	2 cut(s)	1278, 4477
BmuI	ACTGGG	2 cut(s)	1278, 4477
BoxI	GACNNNNGTC	2 cut(s)	2361, 3097
BpiI	GAAGAC	3 cut(s)	2658, 4560, 5277
BplI	GAGNNNNNCTC	6 cut(s)	532, 564, 3223, 3255, 4451, 4483
BpmI	CTGGAG	2 cut(s)	1795, 2544
Bpu10I	CCTNAGC	1 cut(s)	895
BpuEI	CTTGAG	4 cut(s)	214, 1206, 4214, 5189
BpuMI	CCSGG	1 cut(s)	4868
BsaAI	YACGTR	2 cut(s)	1477, 1718
BsaBI	GATNNNNATC	4 cut(s)	648, 3936, 4218, 4224
BsaHI	GRCGYC	2 cut(s)	2323, 2663
BsaI	GGTCTC	1 cut(s)	2791
BsaWI	WCCGGW	5 cut(s)	1115, 1358, 1986, 2035, 2263
BsaXI	ACNNNNNCTCC	2 cut(s)	1264, 1294
Bse118I	RCCGGY	1 cut(s)	1358
Bse3DI	GCAATG	5 cut(s)	801, 1932, 2827, 3860, 4131
Bse8I	GATNNNNATC	4 cut(s)	648, 3936, 4218, 4224
BseAI	TCCGGA	2 cut(s)	1986, 2263
BseJI	GATNNNNATC	4 cut(s)	648, 3936, 4218, 4224
BseMI	GCAATG	5 cut(s)	801, 1932, 2827, 3860, 4131
BseRI	GAGGAG	5 cut(s)	1695, 1740, 3082, 3247, 4475
BseSI	GKGCMC	4 cut(s)	775, 3794, 4276, 4293
BseX3I	CGGCCG	1 cut(s)	3095
BsgI	GTGCAG	3 cut(s)	2598, 2799, 5172
Bsh1236I	CGCG	1 cut(s)	1599
Bsh1285I	CGRYCG	1 cut(s)	3098
BshTI	ACCGGT	1 cut(s)	1358
BsiEI	CGRYCG	1 cut(s)	3098
BsiHKCI	CYCGRG	1 cut(s)	1291
BsiSI	CCGG	8 cut(s)	1116, 1359, 1987, 2036, 2264, 3155, 3905, 4867
BslFI	GGGAC	7 cut(s)	1122, 1128, 2092, 3478, 3580, 3976, 5356
BsmAI	GTCTC	8 cut(s)	236, 2287, 2791, 3200, 3760, 4504, 4949, 5354
BsmBI	CGTCTC	1 cut(s)	2287
BsmFI	GGGAC	7 cut(s)	1122, 1128, 2092, 3478, 3580, 3976, 5356
BsmI	GAATGC	3 cut(s)	1840, 3635, 5044
Bso31I	GGTCTC	1 cut(s)	2791
BsoBI	CYCGRG	1 cut(s)	1291
Bsp120I	GGGCCC	1 cut(s)	3790
Bsp13I	TCCGGA	2 cut(s)	1986, 2263
Bsp1407I	TGTACA	3 cut(s)	343, 1300, 4935
Bsp19I	CCATGG	3 cut(s)	2445, 4188, 4913
Bsp68I	TCGCGA	1 cut(s)	1599
BspACI	CCGC	6 cut(s)	1105, 1729, 2781, 3379, 3846, 3951
BspEI	TCCGGA	2 cut(s)	1986, 2263
BspFNI	CGCG	1 cut(s)	1599
BspHI	TCATGA	8 cut(s)	1432, 1573, 2658, 3028, 3037, 3889, 4222, 4381
BspMAI	CTGCAG	2 cut(s)	2576, 4141
BspMI	ACCTGC	1 cut(s)	22
BspQI	GCTCTTC	1 cut(s)	150
BspTNI	GGTCTC	1 cut(s)	2791
BsrDI	GCAATG	5 cut(s)	801, 1932, 2827, 3860, 4131
BsrFI	RCCGGY	1 cut(s)	1358
BsrGI	TGTACA	3 cut(s)	343, 1300, 4935
BssAI	RCCGGY	1 cut(s)	1358
BssNAI	GTATAC	1 cut(s)	4260
BssNI	GRCGYC	2 cut(s)	2323, 2663
BssSI	CACGAG	2 cut(s)	1665, 1752
BssT1I	CCWWGG	7 cut(s)	2098, 2445, 4188, 4253, 4562, 4913, 4973
Bst1107I	GTATAC	1 cut(s)	4260
Bst2BI	CACGAG	2 cut(s)	1665, 1752
Bst6I	CTCTTC	5 cut(s)	150, 1530, 2285, 2800, 3730
BstACI	GRCGYC	2 cut(s)	2323, 2663
BstAPI	GCANNNNNTGC	5 cut(s)	797, 1334, 1931, 2822, 3689
BstAUI	TGTACA	3 cut(s)	343, 1300, 4935
BstBAI	YACGTR	2 cut(s)	1477, 1718
BstDSI	CCRYGG	4 cut(s)	979, 2445, 4188, 4913
BstEII	GGTNACC	1 cut(s)	3167
BstENI	CCTNNNNNAGG	1 cut(s)	3704
BstFNI	CGCG	1 cut(s)	1599
BstH2I	RGCGCY	1 cut(s)	1075
BstHHI	GCGC	2 cut(s)	1074, 5257
BstMAI	GTCTC	8 cut(s)	236, 2287, 2791, 3200, 3760, 4504, 4949, 5354
BstMCI	CGRYCG	1 cut(s)	3098
BstNSI	RCATGY	5 cut(s)	421, 691, 1981, 3265, 4390
BstPAI	GACNNNNGTC	2 cut(s)	2361, 3097
BstPI	GGTNACC	1 cut(s)	3167
BstSFI	CTRYAG	6 cut(s)	1497, 2572, 3825, 4137, 4593, 5375
BstSLI	GKGCMC	4 cut(s)	775, 3794, 4276, 4293
BstSNI	TACGTA	1 cut(s)	1477
BstUI	CGCG	1 cut(s)	1599
BstV2I	GAAGAC	3 cut(s)	2658, 4560, 5277
BstXI	CCANNNNNNTGG	2 cut(s)	3406, 3518
BstZ17I	GTATAC	1 cut(s)	4260
BstZI	CGGCCG	1 cut(s)	3095
BsuI	GTATCC	2 cut(s)	498, 3115
BtgI	CCRYGG	4 cut(s)	979, 2445, 4188, 4913
BtgZI	GCGATG	1 cut(s)	1614
BtsI	GCAGTG	5 cut(s)	2581, 3282, 3687, 4146, 4347
BtuMI	TCGCGA	1 cut(s)	1599
BveI	ACCTGC	1 cut(s)	22
CaiI	CAGNNNCTG	3 cut(s)	3229, 3470, 4643
CciI	TCATGA	8 cut(s)	1432, 1573, 2658, 3028, 3037, 3889, 4222, 4381
CfoI	GCGC	2 cut(s)	1074, 5257
Cfr10I	RCCGGY	1 cut(s)	1358
CseI	GACGC	1 cut(s)	2312
CspAI	ACCGGT	1 cut(s)	1358
DraI	TTTAAA	2 cut(s)	1555, 2337
DrdI	GACNNNNNNGTC	1 cut(s)	5368
DriI	GACNNNNNGTC	1 cut(s)	2044
DseDI	GACNNNNNNGTC	1 cut(s)	5368
EaeI	YGGCCR	7 cut(s)	969, 1453, 2071, 2095, 2752, 3095, 4864
EagI	CGGCCG	1 cut(s)	3095
Eam1104I	CTCTTC	5 cut(s)	150, 1530, 2285, 2800, 3730
Eam1105I	GACNNNNNGTC	1 cut(s)	2044
EarI	CTCTTC	5 cut(s)	150, 1530, 2285, 2800, 3730
Ecl136II	GAGCTC	8 cut(s)	70, 1017, 2306, 3219, 3237, 3365, 4172, 4467
EclXI	CGGCCG	1 cut(s)	3095
Eco105I	TACGTA	1 cut(s)	1477
Eco130I	CCWWGG	7 cut(s)	2098, 2445, 4188, 4253, 4562, 4913, 4973
Eco147I	AGGCCT	3 cut(s)	676, 1150, 4617
Eco31I	GGTCTC	1 cut(s)	2791
Eco32I	GATATC	1 cut(s)	392
Eco47I	GGWCC	5 cut(s)	323, 1283, 1855, 3357, 4185
Eco52I	CGGCCG	1 cut(s)	3095
Eco53kI	GAGCTC	8 cut(s)	70, 1017, 2306, 3219, 3237, 3365, 4172, 4467
Eco57I	CTGAAG	7 cut(s)	1239, 2344, 2529, 4749, 5105, 5243, 5271
Eco72I	CACGTG	1 cut(s)	1718
Eco88I	CYCGRG	1 cut(s)	1291
Eco91I	GGTNACC	1 cut(s)	3167
EcoICRI	GAGCTC	8 cut(s)	70, 1017, 2306, 3219, 3237, 3365, 4172, 4467
EcoNI	CCTNNNNNAGG	1 cut(s)	3704
EcoO109I	RGGNCCY	3 cut(s)	1724, 3790, 3791
EcoO65I	GGTNACC	1 cut(s)	3167
EcoRI	GAATTC	2 cut(s)	1216, 1737
EcoRV	GATATC	1 cut(s)	392
EcoT14I	CCWWGG	7 cut(s)	2098, 2445, 4188, 4253, 4562, 4913, 4973
EcoT22I	ATGCAT	4 cut(s)	309, 423, 1840, 4392
ErhI	CCWWGG	7 cut(s)	2098, 2445, 4188, 4253, 4562, 4913, 4973
Esp3I	CGTCTC	1 cut(s)	2287
FalI	AAGNNNNNCTT	4 cut(s)	99, 131, 5216, 5248
FaqI	GGGAC	7 cut(s)	1122, 1128, 2092, 3478, 3580, 3976, 5356
FauI	CCCGC	2 cut(s)	1098, 1736
FauNDI	CATATG	2 cut(s)	1888, 3213
FbaI	TGATCA	3 cut(s)	2128, 2710, 4378
FblI	GTMKAC	2 cut(s)	3850, 4259
FspI	TGCGCA	1 cut(s)	5256
GlaI	GCGC	2 cut(s)	1073, 5256
GsuI	CTGGAG	2 cut(s)	1795, 2544
HaeII	RGCGCY	1 cut(s)	1075
HapII	CCGG	8 cut(s)	1116, 1359, 1987, 2036, 2264, 3155, 3905, 4867
HgaI	GACGC	1 cut(s)	2312
HhaI	GCGC	2 cut(s)	1074, 5257
Hin1I	GRCGYC	2 cut(s)	2323, 2663
Hin6I	GCGC	2 cut(s)	1072, 5255
HinP1I	GCGC	2 cut(s)	1072, 5255
HincII	GTYRAC	2 cut(s)	277, 2020
HindII	GTYRAC	2 cut(s)	277, 2020
HindIII	AAGCTT	2 cut(s)	518, 2760
HpaII	CCGG	8 cut(s)	1116, 1359, 1987, 2036, 2264, 3155, 3905, 4867
Hpy99I	CGWCG	1 cut(s)	2220
HpyCH4IV	ACGT	6 cut(s)	1476, 1717, 2045, 2663, 3853, 4922
HpySE526I	ACGT	6 cut(s)	1476, 1717, 2045, 2663, 3853, 4922
Hsp92I	GRCGYC	2 cut(s)	2323, 2663
HspAI	GCGC	2 cut(s)	1072, 5255
Kpn2I	TCCGGA	2 cut(s)	1986, 2263
Ksp22I	TGATCA	3 cut(s)	2128, 2710, 4378
LguI	GCTCTTC	1 cut(s)	150
MaeII	ACGT	6 cut(s)	1476, 1717, 2045, 2663, 3853, 4922
MfeI	CAATTG	4 cut(s)	1131, 1953, 3897, 4782
MlsI	TGGCCA	4 cut(s)	971, 1455, 2073, 2097
MluNI	TGGCCA	4 cut(s)	971, 1455, 2073, 2097
MlyI	GAGTC	8 cut(s)	1222, 1424, 1786, 2114, 3202, 3998, 4714, 5291
MmeI	TCCRAC	6 cut(s)	1044, 1398, 1640, 1769, 3316, 4134
Mox20I	TGGCCA	4 cut(s)	971, 1455, 2073, 2097
Mph1103I	ATGCAT	4 cut(s)	309, 423, 1840, 4392
MroI	TCCGGA	2 cut(s)	1986, 2263
MroXI	GAANNNNTTC	2 cut(s)	2029, 2761
MscI	TGGCCA	4 cut(s)	971, 1455, 2073, 2097
MslI	CAYNNNNRTG	6 cut(s)	234, 768, 990, 1461, 2115, 3144
Msp20I	TGGCCA	4 cut(s)	971, 1455, 2073, 2097
MspA1I	CMGCKG	5 cut(s)	320, 593, 975, 1520, 3951
MspI	CCGG	8 cut(s)	1116, 1359, 1987, 2036, 2264, 3155, 3905, 4867
MunI	CAATTG	4 cut(s)	1131, 1953, 3897, 4782
Mva1269I	GAATGC	3 cut(s)	1840, 3635, 5044
MvnI	CGCG	1 cut(s)	1599
NciI	CCSGG	1 cut(s)	4868
NcoI	CCATGG	3 cut(s)	2445, 4188, 4913
NdeI	CATATG	2 cut(s)	1888, 3213
NmeAIII	GCCGAG	1 cut(s)	3625
NruI	TCGCGA	1 cut(s)	1599
NsbI	TGCGCA	1 cut(s)	5256
NsiI	ATGCAT	4 cut(s)	309, 423, 1840, 4392
NspI	RCATGY	5 cut(s)	421, 691, 1981, 3265, 4390
PaeI	GCATGC	2 cut(s)	421, 1981
PaeR7I	CTCGAG	1 cut(s)	1291
PagI	TCATGA	8 cut(s)	1432, 1573, 2658, 3028, 3037, 3889, 4222, 4381
PceI	AGGCCT	3 cut(s)	676, 1150, 4617
PciSI	GCTCTTC	1 cut(s)	150
PcsI	WCGNNNNNNNCGW	4 cut(s)	1424, 1663, 2623, 3006
PctI	GAATGC	3 cut(s)	1840, 3635, 5044
PdmI	GAANNNNTTC	2 cut(s)	2029, 2761
PflFI	GACNNNGTC	2 cut(s)	327, 4183
PflMI	CCANNNNNTGG	3 cut(s)	2451, 4216, 4643
PfoI	TCCNGGA	1 cut(s)	4636
PinAI	ACCGGT	1 cut(s)	1358
PleI	GAGTC	8 cut(s)	1222, 1423, 1785, 2113, 3202, 3997, 4714, 5290
PmaCI	CACGTG	1 cut(s)	1718
PmlI	CACGTG	1 cut(s)	1718
PpsI	GAGTC	8 cut(s)	1222, 1423, 1785, 2113, 3202, 3997, 4714, 5290
Ppu21I	YACGTR	2 cut(s)	1477, 1718
PshAI	GACNNNNGTC	2 cut(s)	2361, 3097
PshBI	ATTAAT	1 cut(s)	5103
PsiI	TTATAA	1 cut(s)	4574
Psp124BI	GAGCTC	8 cut(s)	72, 1019, 2308, 3221, 3239, 3367, 4174, 4469
PspCI	CACGTG	1 cut(s)	1718
PspEI	GGTNACC	1 cut(s)	3167
PspOMI	GGGCCC	1 cut(s)	3790
PspXI	VCTCGAGB	1 cut(s)	1291
PstI	CTGCAG	2 cut(s)	2576, 4141
PstNI	CAGNNNCTG	3 cut(s)	3229, 3470, 4643
PsyI	GACNNNGTC	2 cut(s)	327, 4183
PvuII	CAGCTG	4 cut(s)	320, 593, 975, 1520
RruI	TCGCGA	1 cut(s)	1599
RseI	CAYNNNNRTG	6 cut(s)	234, 768, 990, 1461, 2115, 3144
SacI	GAGCTC	8 cut(s)	72, 1019, 2308, 3221, 3239, 3367, 4174, 4469
SapI	GCTCTTC	1 cut(s)	150
SchI	GAGTC	8 cut(s)	1222, 1424, 1786, 2114, 3202, 3998, 4714, 5291
SfcI	CTRYAG	6 cut(s)	1497, 2572, 3825, 4137, 4593, 5375
Sfr274I	CTCGAG	1 cut(s)	1291
SinI	GGWCC	5 cut(s)	323, 1283, 1855, 3357, 4185
SlaI	CTCGAG	1 cut(s)	1291
SmiMI	CAYNNNNRTG	6 cut(s)	234, 768, 990, 1461, 2115, 3144
SmlI	CTYRAG	5 cut(s)	193, 1221, 1291, 4229, 5168
SmoI	CTYRAG	5 cut(s)	193, 1221, 1291, 4229, 5168
SnaBI	TACGTA	1 cut(s)	1477
SpeI	ACTAGT	1 cut(s)	3569
SphI	GCATGC	2 cut(s)	421, 1981
SseBI	AGGCCT	3 cut(s)	676, 1150, 4617
SsiI	CCGC	6 cut(s)	1105, 1729, 2781, 3379, 3846, 3951
SspI	AATATT	3 cut(s)	885, 2503, 2932
SstI	GAGCTC	8 cut(s)	72, 1019, 2308, 3221, 3239, 3367, 4174, 4469
StuI	AGGCCT	3 cut(s)	676, 1150, 4617
StyI	CCWWGG	7 cut(s)	2098, 2445, 4188, 4253, 4562, 4913, 4973
TaiI	ACGT	6 cut(s)	1479, 1720, 2048, 2666, 3856, 4925
TaqII	GACCGA	1 cut(s)	2432
TatI	WGTACW	6 cut(s)	343, 542, 1300, 2279, 2496, 4935
TauI	GCSGC	2 cut(s)	3382, 3954
TspGWI	ACGGA	4 cut(s)	2037, 2055, 2958, 5317
Tth111I	GACNNNGTC	2 cut(s)	327, 4183
Van91I	CCANNNNNTGG	3 cut(s)	2451, 4216, 4643
VneI	GTGCAC	2 cut(s)	771, 4289
VpaK11BI	GGWCC	5 cut(s)	323, 1283, 1855, 3357, 4185
VspI	ATTAAT	1 cut(s)	5103
XagI	CCTNNNNNAGG	1 cut(s)	3704
XbaI	TCTAGA	3 cut(s)	1505, 2365, 5323
XceI	RCATGY	5 cut(s)	421, 691, 1981, 3265, 4390
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1027, 1865, 4911
XhoI	CTCGAG	1 cut(s)	1291
XmaJI	CCTAGG	1 cut(s)	4253
XmiI	GTMKAC	2 cut(s)	3850, 4259
XmnI	GAANNNNTTC	2 cut(s)	2029, 2761
ZraI	GACGTC	1 cut(s)	2664
Zsp2I	ATGCAT	4 cut(s)	309, 423, 1840, 4392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.