Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0003731

Physical Location & Seq

Genomic Coordinates Reverse (-)

48420189 .. 48435651

Gene Structure 15463 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00366520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4908 bp

ATGCGGAAGCGGGCGACGCGGGAGCGATGGGATTCGGCGATCGTCGTACGGAACACAAGACGAGCCGGTTTTACAGGAGCTATGGCTCTTCAAAAGTTTCCTTGTGGTGATGCACCTTCTGTTAGTATGAAACCCTTGAGCTTTCTCAGTAAGGTGCATGACAGCACTCTACATGAGGATGTAGAGACAAATCATGCCAGGGAACAAGATGTGGATGTTGACCTCAGAGAAGTGTATTTCCTCATTATGCATTTTCTTTCAGCTGGACCATGTCATAAAACTTGTGTACAGTTCTGGAACGAACTTCTTGAACATCAACTTTTACCTAGAAGATATCACGCATGGTATTCAAGGAAAGGCATGCATAGTGGGGATGAAAATGATGATGGGCAATCATTCCCCATAAATTACAATATGTTGGTGGCAAGGTATCCTCACATTGAGCAGGATCACTTGGTAAAGCTTTTGAAGCAAATGATTTTGAGTACAGCTCCACCATCAAGGGGTATGAGTGGTGGAAATGCCCCTAATGCAGCTGATGTACCAACTCTCTTGGGAACGGGCTCATTTTCACTTCTGAGCTATGATAGGGATCAAGAAAACAACGAAATGAACCGGCCTGCTGCTTACATGCGTTGGCCTCACATCAAAGCTAATCAGGTGCATGGGCTAGGTTTGAGGGAAATTGGGGGAGGTTTCACTAGACATCACCGTGCACCATCTGTTCGTGCTGCATCCTATGCCATTGCGAAACCATCAACCATGTTTCAGAAGATGAAAAATACCAAGAGAATAAGGGGCCATCGAAACGCTGTCTATTGTGCAATATTTGATCGCTCAGGTAGATATGTTATCACTGGTTCAGATGATCGCCTAGTGAAGATCTGGTTAATAGAAACTGCATTTTGCTTGGCCAGCTGCCGTGGACATGAAGGTGACATCACTGATTTAGCTGTGAGCTCCAACAATGCTTTGGTGGCATCCGCTTCAAATGACTGTATCATACGAGTTTGGCGCTTGCCAGATGGGCTTCCAATTTCGGTGTTGCGGGGACACACCGGAGCTGTGACTGCAATTGCATTTAATCCCAGGCCTGGCTCTATATACCACCTTCTATCTTCCTCTGATGATGGAACTTGTAGGATATGGGATGCCAGGAATTCTCAAGTGACTCCGAGGATATACATTCCAAAACCTTCAGATACTATAACTGGGAGAAACAGTGGTCCATCCTCAAGCACTGTACGGAGCCATCAAATCTTCTGTTGTGCATTTAATGCTAATGGAACAGTCTTTGTTACCGGTAGCTCAGACACTCTTGCGAGGGTGTGGAGTGCTTCCAAGCCAGGCTCAGATGAGTCAGACCAACCGAATCATGAAATAGATGTGTTATCTGGCCATGAGAATGATGTGAATTACGTACAATTTAGTGGTTGTGCTGTAGTATCTAGATTTATGGCAGCTGACACCTCAAAGGAAGAGAATGTTCCAAAATTTAAAAATTCATGGTTCAATCATGACAATATAGTTACTTGCTCTCGCGATGGGAGTGCAATTATCTGGATTCCAAAGTCACGAAGATCACATGGAAAAGCTGGTCGTTGGACACGAGCTTATCATCTGAAAGTTCCACCTCCACCAATGCCTCCTCAACCTTCACGTGGAGGCCCCCGTCAGAGAATTCTTCCAACTCCTCGTGGTGTTAATATGATTACCTGGAGTCTTGATAATCGCTTTGTTCTTGCTGCTATCATGGATTGCAGAATATGTGTATGGAATGCATCTGATGGTAGCTTGGTCCATTCTTTAACTGGTCACAGTGATTCTACATATGTTCTGGATGTTCACCCTTTCAACCCCAGGTTGGCAATGAGTGCTGGCTATGATGGAAAAACAATTGTCTGGGATTGGCCAAGGGAGTCCCACAATGATGCAAAATATGATCAGTTCTTTCTGGGTGATTATCGCCCAATCATTCAAGATAGCTTCGGAAATGTTCTTGACCAGGAAACTCAGATTCCAGCATACCGACGAAATATGCAGGATCTACTTTGTGATTCAGGCATGATACCATATCCGGAGCCCTACCAGAGTACATACCAGAAGAGACGGCTAGGAGCTCTGGGTTCTGAGTGGCGTCCTTCCTCTTTAAAACTTGCTGTTGGGCCTGACTTCAGTCTAGATCCAGATTTCCAAATGCTACCTTTGGCAGACTTGGATATGTTATTGACCGAGCCACTACCAGAGTTTGTAGATGCCATGAATTGGGAACCAGAAAATGAAATGCATAGTGATGATGCTGATTCAGAGTACAATATTACTGAAGATTATTCTACTGGAGGGGAGCAAGGAAGTTTAAGTTCAAACCCTTCTGTTGATCCAGACTGCAGTGCAGAAGATAGTGACGAGGATGCTCAACTGGATGGACTACGGAGATCAAGAAGGAAAAAACAAAAGGCTGATATTGAAGTCATGACGTCTTCTGGAAGATGTGTTAAAAGGAGGAACTTGGATGAGCGTGATGATCACTCTTTCAGAAATACTGATGGAGAAGATGAAGATGGATCCGAAGGAGAAATTTCAGGTAGTGAATCCACGCTGCAAGATTCAAATATTGAGAGTGACGGATCCGATAGGGAAAATCACCAAAGCAACTATATGAAAGGAAAACAGGTTTTACTCGATGAATCTGAGGGTCTGGTCAATTCTCATGAACTTCCTGAATTTCAAAGAAACTCTGGGAACAGGAGGAGATTAGTGCTAAAACTGCCAAGACGGCCGTCAAATACAAATACGCTTGTGGATACAGATAAGTGTGGAAATCCGGCTTATTTGGGTGACCAATCTTGTAGTCACAAAGCCAGTGAAGCAAATGAGACTCATATGAGCTCTCAGGATCTGAGGAGCTCTCCTGGTCATAAAAATTGCAGCATGTTTGAAAGAGCAGTGGGAGGGCAATCAAACAAAGTTGAAAATTATTTAGACTTGACTGAGAATTACAAAGTTGGAAGTATAAGATGGGGAGGGTCCAGAGCTCGAACATTGAAGCGGCTGAGAGTGGGAGAAGCCACATCACTGGATGCACTTGCCACTACCAGTATAGCTGTTGTTGGTCACACCGAAAAAAATTACACAGAATCTGAAAAAGATTGTCGAAAAATGTCCCCTCAATTAGAAAGCCAAAGGAATGGGGATATGATGGATGGAGTGGTTATGACGAATGAGAGAACTATTGGGACTAGTACCTTTGAGCACTCAGGATTTAGTGAATGTAAGGATCATGACCAATCACCTAAATCTGTTCACATGGCTCCTTGGGATGCAAGCACTGCCTCTTACCTTGACAAGGATGGGAGTGTTTTCTCTTCTGAACAAAACGAGAAGCTCACACCGGTCTCGACAAAATTAAGGTTGAGGAAGATTTTGAGGGGCCCTGAAAGTCCTTGCAAACAGGAAATGAGCCCTGTAGTAGAGAACTTGGAAAGCGGTAGATGTAACACATTGCCTGAAAGCCTTTCAACAATAGATCATGATTCAATTGTGCCGGAGGATAATGGCACCCATAAATTTGATTCAGATCAAAGATGCAGCGGCTCACGAGAATCAGACACTCAAATTGAATCAGTCGAGTCCCATCTAACTAGAAACAAAATGTTTATGGACTGCCGTGACGATTCAATTGATGGAGCTCGTAGGACACGGTCCATGGGGTTGAAGGCATCTACCCGTGATCCAAGTAGTGTGGATCATGATCTCAAGTTAAGTCAAGGCCATGAGCCTAGGTATACATTCAGAAGTGGCCAGAACAACACTCTGCACAAATGTCAATTTCAGAATGAAGAACAGGGATCAAGTTCAAGGACAGCAGTGGGGTTGAGATCTACTAGAAACAGAAGGAGTTATTATGATCATGACATGCATCCAATAGATAAAAGGAAGTCGCATCAATCAACGAGGAAAGGAACATGGTTGATGTTGTCCGCACATGAGGAGAGCTCACGATATATTCCCCAGTTGGGGGATGAAGTTGTCTATTTGAGACAGGGGCATCAGGAGTATTTTGATCTTGGTAGGTTGAGAGAAGACCCACCTTGGGCATTTATAAAGCAAAGAATAAGAGCTGTGGAATTTTGTAAAGTTCAAGACCTAGAGTATTCTTTACTTCCAGGGTCTGGGGATAGCTGCTGTAAATTGACCCTTGAATTTGTAGATCCTTCATCTGATGTTTACTGCAAATCTTTCAAAATGACTCTGCCTGAAGTTACTGGTTTCCCCGATTTCATTGTTGAAAGAACTCGCTATGTTGCTGCAATTCAGAAAAAATGGTCATGTAGGGATAAGTGCAAAGTCTGGTGGAAAAATGACGGTGACAATGATGGTAGTTGGTGGGATGGCCGGGTTGTGCTTATTCAGCCCAAATCTCCTGAATTTCCAGACAGTCCATGGGAAACGTGTACGGTTCTGTACAGGACTGTTCCTAAAGAGACACAGCTACATAGTCCTTGGGAGCTTTTTGATACCGATACTCAATGGGTTCAGCCTCAGATTGATGACAAGAGCAAAAACAAGCTGCATTCTGCCTTTGCCAAACTAGAGAAATTAGCCAGCAGTCGTCAGGATTCATTAGGGATTAATAAATTGAAGCAGCTTCAGGAGAAGCCGAAGTTCACAAATTGGTGTGCAGTTCCAATATCTCTTGACTTGATCCAGAGTAGGTTAGAAAACGACTATTATCGAACTTTGGAAGCGCTGAAGCATGACTTTAAGGTAATGTTGTTAGATGCGCAAGCCTATGTAGAAAGCCATGCGGAGTCTTCAGGCAAAACGAAACGGACAGCAGATAAAGAGTTTCTAGAGAAACTGAAATGCCTATCAGGCTGGTTCACAGAGACAATTTCGTCCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008360 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019221 GO:0019222 GO:0022603 GO:0022604 GO:0023052 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0034097 GO:0038111 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071345 GO:0071840 GO:0080090 GO:0098760 GO:0098761 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_resp Bromodomain WD40

Protein Analysis

1635

Amino Acids

184.52

Weight (kDa)

6.31

Isoelectric Point (pI)

51.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRWD1_N	PF25437	63 - 163	2.3e-34	BRWD1-like, N-terminal domain
Beta-prop_TEP1_2nd	PF25047	255 - 391	2.4e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	256 - 343	9.6e-18	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	257 - 391	4.8e-15	CDC20/Fizzy WD40 domain
WDR55	PF24796	257 - 390	2.1e-07	WDR55
Beta-prop_WDR3_1st	PF25173	258 - 389	3.5e-22	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	259 - 448	1e-13	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	259 - 342	8.9e-11	Prp19 WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	260 - 340	3.3e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	261 - 391	9.7e-27	WDR5 beta-propeller domain
WD40	PF00400	261 - 296	6.7e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	263 - 339	7.4e-10	WDR3 second beta-propeller domain
Beta-prop_SCAP	PF24017	280 - 385	2.7e-09	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	300 - 363	9.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	302 - 395	1.8e-08	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	303 - 338	1.5e-07	WD domain, G-beta repeat
EIF3I	PF24805	308 - 392	3.8e-06	EIF3I
Beta-prop_THOC3	PF25174	310 - 393	4e-12	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	313 - 401	2.4e-12	WDHD1 first WD40 domain
WD40	PF00400	345 - 384	9e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	566 - 638	8.7e-08	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	568 - 638	3.9e-07	THOC3 beta-propeller domain
BRWD_AD	PF25313	1333 - 1509	2.5e-55	BRWD/PHIP ancillary domain-like domain
Bromodomain	PF00439	1550 - 1601	1.7e-06	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49430 AT5G49430
fragaria_vesca	FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490 FvH4_4g19490
malus_domestica	MD13G1156100.v1.1 MD16G1157000.v1.1
prunus_persica	Prupe.1G121900_v2.0.a1 Prupe.1G121900_v2.0.a1
pyrus_communis	pycom13g13310 pycom13g13320 pycom13g13330 pycom13g13340 pycom13g13350 pycom16g13190 pycom16g13200
rosa_chinensis	RchiOBHm_Chr4g0424601
rosa_laevigata	RLG00000007452
rosa_multiflora	Rmu_co8472673.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366520
rosa_rugosa	Rorug04G0198100 Rorug04G0198200 Rorug04G0198300
rosa_samantha	Rh4AG254900 Rh4BG260400 Rh4CG271900 Rh4DG255100
rosa_wichuraiana	Rw4G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4103
AasI	GACNNNNNNGTC	1 cut(s)	4897
AatII	GACGTC	1 cut(s)	2480
Acc16I	TGCGCA	1 cut(s)	4785
AccB1I	GGYRCC	1 cut(s)	3557
AccB7I	CCANNNNNTGG	2 cut(s)	2265, 4172
AccI	GTMKAC	1 cut(s)	3785
AccII	CGCG	2 cut(s)	19, 1542
AccIII	TCCGGA	1 cut(s)	2077
AcoI	YGGCCR	6 cut(s)	912, 1396, 1910, 2777, 3799, 4393
AcuI	CTGAAG	7 cut(s)	1182, 2158, 2343, 4278, 4634, 4772, 4800
AcvI	CACGTG	1 cut(s)	1661
AcyI	GRCGYC	2 cut(s)	2137, 2477
AfeI	AGCGCT	1 cut(s)	4749
AflIII	ACRYGT	1 cut(s)	4451
AgeI	ACCGGT	2 cut(s)	1301, 3391
AhlI	ACTAGT	1 cut(s)	3239
Alw21I	GWGCWC	9 cut(s)	718, 962, 2122, 2891, 2909, 3037, 3255, 3691, 3998
Alw26I	GTCTC	7 cut(s)	179, 2101, 2870, 3400, 4033, 4478, 4883
Alw44I	GTGCAC	1 cut(s)	714
AlwNI	CAGNNNCTG	3 cut(s)	2899, 3140, 4172
Aor13HI	TCCGGA	1 cut(s)	2077
Aor51HI	AGCGCT	1 cut(s)	4749
ApaI	GGGCCC	1 cut(s)	3434
ApaLI	GTGCAC	1 cut(s)	714
AseI	ATTAAT	1 cut(s)	4632
AsiGI	ACCGGT	2 cut(s)	1301, 3391
AspA2I	CCTAGG	1 cut(s)	3779
AspLEI	GCGC	3 cut(s)	1017, 4750, 4786
AsuC2I	CCSGG	1 cut(s)	4397
AsuHPI	GGTGA	9 cut(s)	119, 701, 947, 1838, 1970, 2636, 2849, 3282, 4379
AvaII	GGWCC	5 cut(s)	266, 1226, 1798, 3027, 3702
AvrII	CCTAGG	1 cut(s)	3779
BaeGI	GKGCMC	2 cut(s)	718, 3434
BalI	TGGCCA	4 cut(s)	914, 1398, 1912, 3801
BamHI	GGATCC	2 cut(s)	2564, 2627
BanI	GGYRCC	1 cut(s)	3557
BauI	CACGAG	3 cut(s)	1608, 1695, 3597
BbrPI	CACGTG	1 cut(s)	1661
BbsI	GAAGAC	3 cut(s)	2472, 4089, 4806
Bbv12I	GWGCWC	9 cut(s)	718, 962, 2122, 2891, 2909, 3037, 3255, 3691, 3998
BceAI	ACGGC	5 cut(s)	906, 2126, 2764, 2792, 3651
BcgI	CGANNNNNNTGC	2 cut(s)	1532, 1566
BciVI	GTATCC	2 cut(s)	441, 2797
BclI	TGATCA	3 cut(s)	1942, 2524, 3907
BcnI	CCSGG	1 cut(s)	4397
BcoDI	GTCTC	7 cut(s)	179, 2101, 2870, 3400, 4033, 4478, 4883
BcuI	ACTAGT	1 cut(s)	3239
BfmI	CTRYAG	4 cut(s)	1440, 2386, 3465, 4904
BfoI	RGCGCY	2 cut(s)	1018, 4751
BfuI	GTATCC	2 cut(s)	441, 2797
BglI	GCCNNNNNGGC	3 cut(s)	1027, 2776, 4875
BglII	AGATCT	2 cut(s)	882, 3878
BlnI	CCTAGG	1 cut(s)	3779
Bme18I	GGWCC	5 cut(s)	266, 1226, 1798, 3027, 3702
BmrI	ACTGGG	2 cut(s)	1221, 4006
BmuI	ACTGGG	2 cut(s)	1221, 4006
BoxI	GACNNNNGTC	2 cut(s)	2175, 2779
BpiI	GAAGAC	3 cut(s)	2472, 4089, 4806
BplI	GAGNNNNNCTC	6 cut(s)	475, 507, 2893, 2925, 3980, 4012
BpmI	CTGGAG	2 cut(s)	1738, 2358
Bpu10I	CCTNAGC	1 cut(s)	838
BpuEI	CTTGAG	4 cut(s)	157, 1149, 1219, 3740
BpuMI	CCSGG	1 cut(s)	4397
BsaAI	YACGTR	2 cut(s)	1420, 1661
BsaBI	GATNNNNATC	3 cut(s)	591, 3576, 3750
BsaHI	GRCGYC	2 cut(s)	2137, 2477
BsaI	GGTCTC	1 cut(s)	3400
BsaWI	WCCGGW	4 cut(s)	1058, 1301, 2077, 3391
BsaXI	ACNNNNNCTCC	2 cut(s)	1207, 1237
Bse118I	RCCGGY	4 cut(s)	65, 615, 1301, 3391
Bse3DI	GCAATG	3 cut(s)	744, 1875, 3500
Bse8I	GATNNNNATC	3 cut(s)	591, 3576, 3750
BseAI	TCCGGA	1 cut(s)	2077
BseJI	GATNNNNATC	3 cut(s)	591, 3576, 3750
BseMI	GCAATG	3 cut(s)	744, 1875, 3500
BseRI	GAGGAG	5 cut(s)	1638, 1683, 2764, 2917, 4004
BseSI	GKGCMC	2 cut(s)	718, 3434
BseX3I	CGGCCG	1 cut(s)	2777
BsgI	GTGCAG	3 cut(s)	2412, 3800, 4701
Bsh1236I	CGCG	2 cut(s)	19, 1542
Bsh1285I	CGRYCG	2 cut(s)	42, 2780
BshNI	GGYRCC	1 cut(s)	3557
BshTI	ACCGGT	2 cut(s)	1301, 3391
BsiEI	CGRYCG	2 cut(s)	42, 2780
BsiHKAI	GWGCWC	9 cut(s)	718, 962, 2122, 2891, 2909, 3037, 3255, 3691, 3998
BsiSI	CCGG	9 cut(s)	66, 616, 1059, 1302, 2078, 2825, 3392, 3545, 4396
BsiWI	CGTACG	1 cut(s)	46
BslFI	GGGAC	6 cut(s)	1065, 1906, 3148, 3250, 3616, 4885
BsmAI	GTCTC	7 cut(s)	179, 2101, 2870, 3400, 4033, 4478, 4883
BsmBI	CGTCTC	1 cut(s)	2101
BsmFI	GGGAC	6 cut(s)	1065, 1906, 3148, 3250, 3616, 4885
BsmI	GAATGC	2 cut(s)	1783, 4573
Bso31I	GGTCTC	1 cut(s)	3400
Bsp120I	GGGCCC	1 cut(s)	3430
Bsp13I	TCCGGA	1 cut(s)	2077
Bsp1407I	TGTACA	2 cut(s)	286, 4464
Bsp19I	CCATGG	2 cut(s)	3705, 4442
Bsp68I	TCGCGA	1 cut(s)	1542
BspEI	TCCGGA	1 cut(s)	2077
BspFNI	CGCG	2 cut(s)	19, 1542
BspHI	TCATGA	8 cut(s)	1375, 1516, 2472, 2710, 3280, 3529, 3748, 3910
BspMAI	CTGCAG	1 cut(s)	2390
BspQI	GCTCTTC	1 cut(s)	93
BspT107I	GGYRCC	1 cut(s)	3557
BspTNI	GGTCTC	1 cut(s)	3400
BsrDI	GCAATG	3 cut(s)	744, 1875, 3500
BsrFI	RCCGGY	4 cut(s)	65, 615, 1301, 3391
BsrGI	TGTACA	2 cut(s)	286, 4464
BssAI	RCCGGY	4 cut(s)	65, 615, 1301, 3391
BssNAI	GTATAC	1 cut(s)	3786
BssNI	GRCGYC	2 cut(s)	2137, 2477
BssSI	CACGAG	3 cut(s)	1608, 1695, 3597
BssT1I	CCWWGG	7 cut(s)	1913, 3314, 3705, 3779, 4091, 4442, 4502
Bst1107I	GTATAC	1 cut(s)	3786
Bst2BI	CACGAG	3 cut(s)	1608, 1695, 3597
Bst6I	CTCTTC	4 cut(s)	93, 1473, 2099, 3370
BstACI	GRCGYC	2 cut(s)	2137, 2477
BstAPI	GCANNNNNTGC	4 cut(s)	740, 1277, 1874, 3329
BstAUI	TGTACA	2 cut(s)	286, 4464
BstBAI	YACGTR	2 cut(s)	1420, 1661
BstC8I	GCNNGC	9 cut(s)	12, 362, 621, 916, 1019, 1879, 3325, 4606, 4788
BstDSI	CCRYGG	3 cut(s)	922, 3705, 4442
BstEII	GGTNACC	1 cut(s)	2837
BstENI	CCTNNNNNAGG	1 cut(s)	3344
BstFNI	CGCG	2 cut(s)	19, 1542
BstH2I	RGCGCY	2 cut(s)	1018, 4751
BstHHI	GCGC	3 cut(s)	1017, 4750, 4786
BstMAI	GTCTC	7 cut(s)	179, 2101, 2870, 3400, 4033, 4478, 4883
BstMCI	CGRYCG	2 cut(s)	42, 2780
BstNSI	RCATGY	4 cut(s)	364, 634, 2935, 3919
BstPAI	GACNNNNGTC	2 cut(s)	2175, 2779
BstPI	GGTNACC	1 cut(s)	2837
BstSFI	CTRYAG	4 cut(s)	1440, 2386, 3465, 4904
BstSLI	GKGCMC	2 cut(s)	718, 3434
BstSNI	TACGTA	1 cut(s)	1420
BstUI	CGCG	2 cut(s)	19, 1542
BstV2I	GAAGAC	3 cut(s)	2472, 4089, 4806
BstX2I	RGATCY	8 cut(s)	882, 2044, 2182, 2564, 2627, 2896, 3878, 4210
BstXI	CCANNNNNNTGG	2 cut(s)	3076, 3188
BstYI	RGATCY	8 cut(s)	882, 2044, 2182, 2564, 2627, 2896, 3878, 4210
BstZ17I	GTATAC	1 cut(s)	3786
BstZI	CGGCCG	1 cut(s)	2777
BsuI	GTATCC	2 cut(s)	441, 2797
BtgI	CCRYGG	3 cut(s)	922, 3705, 4442
BtgZI	GCGATG	2 cut(s)	40, 1557
BtsI	GCAGTG	4 cut(s)	2395, 2952, 3327, 3873
BtuMI	TCGCGA	1 cut(s)	1542
Cac8I	GCNNGC	9 cut(s)	12, 362, 621, 916, 1019, 1879, 3325, 4606, 4788
CaiI	CAGNNNCTG	3 cut(s)	2899, 3140, 4172
CciI	TCATGA	8 cut(s)	1375, 1516, 2472, 2710, 3280, 3529, 3748, 3910
CfoI	GCGC	3 cut(s)	1017, 4750, 4786
Cfr10I	RCCGGY	4 cut(s)	65, 615, 1301, 3391
CseI	GACGC	2 cut(s)	25, 2126
CspAI	ACCGGT	2 cut(s)	1301, 3391
CspCI	CAANNNNNGTGG	2 cut(s)	3723, 3758
DraI	TTTAAA	2 cut(s)	1498, 2151
DrdI	GACNNNNNNGTC	1 cut(s)	4897
DseDI	GACNNNNNNGTC	1 cut(s)	4897
EaeI	YGGCCR	6 cut(s)	912, 1396, 1910, 2777, 3799, 4393
EagI	CGGCCG	1 cut(s)	2777
Eam1104I	CTCTTC	4 cut(s)	93, 1473, 2099, 3370
EarI	CTCTTC	4 cut(s)	93, 1473, 2099, 3370
Ecl136II	GAGCTC	7 cut(s)	960, 2120, 2889, 2907, 3035, 3689, 3996
EclXI	CGGCCG	1 cut(s)	2777
Eco105I	TACGTA	1 cut(s)	1420
Eco130I	CCWWGG	7 cut(s)	1913, 3314, 3705, 3779, 4091, 4442, 4502
Eco147I	AGGCCT	1 cut(s)	1093
Eco31I	GGTCTC	1 cut(s)	3400
Eco32I	GATATC	1 cut(s)	335
Eco47I	GGWCC	5 cut(s)	266, 1226, 1798, 3027, 3702
Eco47III	AGCGCT	1 cut(s)	4749
Eco52I	CGGCCG	1 cut(s)	2777
Eco53kI	GAGCTC	7 cut(s)	960, 2120, 2889, 2907, 3035, 3689, 3996
Eco57I	CTGAAG	7 cut(s)	1182, 2158, 2343, 4278, 4634, 4772, 4800
Eco72I	CACGTG	1 cut(s)	1661
Eco91I	GGTNACC	1 cut(s)	2837
EcoICRI	GAGCTC	7 cut(s)	960, 2120, 2889, 2907, 3035, 3689, 3996
EcoNI	CCTNNNNNAGG	1 cut(s)	3344
EcoO109I	RGGNCCY	3 cut(s)	1667, 3430, 3431
EcoO65I	GGTNACC	1 cut(s)	2837
EcoRI	GAATTC	2 cut(s)	1159, 1680
EcoRV	GATATC	1 cut(s)	335
EcoT14I	CCWWGG	7 cut(s)	1913, 3314, 3705, 3779, 4091, 4442, 4502
EcoT22I	ATGCAT	5 cut(s)	252, 366, 1783, 2289, 3921
ErhI	CCWWGG	7 cut(s)	1913, 3314, 3705, 3779, 4091, 4442, 4502
Esp3I	CGTCTC	1 cut(s)	2101
FalI	AAGNNNNNCTT	2 cut(s)	4745, 4777
FaqI	GGGAC	6 cut(s)	1065, 1906, 3148, 3250, 3616, 4885
FauI	CCCGC	3 cut(s)	3, 12, 1041
FauNDI	CATATG	2 cut(s)	1831, 2883
FbaI	TGATCA	3 cut(s)	1942, 2524, 3907
FblI	GTMKAC	1 cut(s)	3785
FspI	TGCGCA	1 cut(s)	4785
GlaI	GCGC	3 cut(s)	1016, 4749, 4785
GsuI	CTGGAG	2 cut(s)	1738, 2358
HaeII	RGCGCY	2 cut(s)	1018, 4751
HapII	CCGG	9 cut(s)	66, 616, 1059, 1302, 2078, 2825, 3392, 3545, 4396
HgaI	GACGC	2 cut(s)	25, 2126
HhaI	GCGC	3 cut(s)	1017, 4750, 4786
Hin1I	GRCGYC	2 cut(s)	2137, 2477
Hin6I	GCGC	3 cut(s)	1015, 4748, 4784
HinP1I	GCGC	3 cut(s)	1015, 4748, 4784
HincII	GTYRAC	1 cut(s)	220
HindII	GTYRAC	1 cut(s)	220
HindIII	AAGCTT	1 cut(s)	461
HpaII	CCGG	9 cut(s)	66, 616, 1059, 1302, 2078, 2825, 3392, 3545, 4396
HphI	GGTGA	9 cut(s)	119, 701, 947, 1838, 1970, 2636, 2849, 3282, 4379
Hpy99I	CGWCG	3 cut(s)	19, 47, 2034
HpyCH4IV	ACGT	4 cut(s)	1419, 1660, 2477, 4451
HpySE526I	ACGT	4 cut(s)	1419, 1660, 2477, 4451
Hsp92I	GRCGYC	2 cut(s)	2137, 2477
HspAI	GCGC	3 cut(s)	1015, 4748, 4784
Kpn2I	TCCGGA	1 cut(s)	2077
Ksp22I	TGATCA	3 cut(s)	1942, 2524, 3907
LguI	GCTCTTC	1 cut(s)	93
MaeII	ACGT	4 cut(s)	1419, 1660, 2477, 4451
MfeI	CAATTG	4 cut(s)	1074, 1896, 3537, 3678
MflI	RGATCY	8 cut(s)	882, 2044, 2182, 2564, 2627, 2896, 3878, 4210
MlsI	TGGCCA	4 cut(s)	914, 1398, 1912, 3801
MluNI	TGGCCA	4 cut(s)	914, 1398, 1912, 3801
MlyI	GAGTC	8 cut(s)	1165, 1367, 1729, 1928, 2872, 3638, 4243, 4820
MmeI	TCCRAC	4 cut(s)	987, 1583, 1712, 2986
Mox20I	TGGCCA	4 cut(s)	914, 1398, 1912, 3801
Mph1103I	ATGCAT	5 cut(s)	252, 366, 1783, 2289, 3921
MroI	TCCGGA	1 cut(s)	2077
MscI	TGGCCA	4 cut(s)	914, 1398, 1912, 3801
MslI	CAYNNNNRTG	6 cut(s)	177, 711, 933, 1404, 1929, 2292
Msp20I	TGGCCA	4 cut(s)	914, 1398, 1912, 3801
MspA1I	CMGCKG	5 cut(s)	263, 536, 918, 1463, 3591
MspI	CCGG	9 cut(s)	66, 616, 1059, 1302, 2078, 2825, 3392, 3545, 4396
MunI	CAATTG	4 cut(s)	1074, 1896, 3537, 3678
Mva1269I	GAATGC	2 cut(s)	1783, 4573
MvnI	CGCG	2 cut(s)	19, 1542
NciI	CCSGG	1 cut(s)	4397
NcoI	CCATGG	2 cut(s)	3705, 4442
NdeI	CATATG	2 cut(s)	1831, 2883
NruI	TCGCGA	1 cut(s)	1542
NsbI	TGCGCA	1 cut(s)	4785
NsiI	ATGCAT	5 cut(s)	252, 366, 1783, 2289, 3921
NspI	RCATGY	4 cut(s)	364, 634, 2935, 3919
PaeI	GCATGC	1 cut(s)	364
PagI	TCATGA	8 cut(s)	1375, 1516, 2472, 2710, 3280, 3529, 3748, 3910
PceI	AGGCCT	1 cut(s)	1093
PciSI	GCTCTTC	1 cut(s)	93
PcsI	WCGNNNNNNNCGW	1 cut(s)	1606
PctI	GAATGC	2 cut(s)	1783, 4573
Pfl23II	CGTACG	1 cut(s)	46
PflFI	GACNNNGTC	2 cut(s)	270, 3700
PflMI	CCANNNNNTGG	2 cut(s)	2265, 4172
PinAI	ACCGGT	2 cut(s)	1301, 3391
Ple19I	CGATCG	1 cut(s)	42
PleI	GAGTC	8 cut(s)	1165, 1366, 1728, 1927, 2872, 3637, 4243, 4819
PmaCI	CACGTG	1 cut(s)	1661
PmlI	CACGTG	1 cut(s)	1661
PpsI	GAGTC	8 cut(s)	1165, 1366, 1728, 1927, 2872, 3637, 4243, 4819
Ppu21I	YACGTR	2 cut(s)	1420, 1661
PshAI	GACNNNNGTC	2 cut(s)	2175, 2779
PshBI	ATTAAT	1 cut(s)	4632
PsiI	TTATAA	1 cut(s)	4103
Psp124BI	GAGCTC	7 cut(s)	962, 2122, 2891, 2909, 3037, 3691, 3998
PspCI	CACGTG	1 cut(s)	1661
PspEI	GGTNACC	1 cut(s)	2837
PspLI	CGTACG	1 cut(s)	46
PspOMI	GGGCCC	1 cut(s)	3430
PstI	CTGCAG	1 cut(s)	2390
PstNI	CAGNNNCTG	3 cut(s)	2899, 3140, 4172
PsuI	RGATCY	8 cut(s)	882, 2044, 2182, 2564, 2627, 2896, 3878, 4210
PsyI	GACNNNGTC	2 cut(s)	270, 3700
PvuI	CGATCG	1 cut(s)	42
PvuII	CAGCTG	4 cut(s)	263, 536, 918, 1463
RruI	TCGCGA	1 cut(s)	1542
RseI	CAYNNNNRTG	6 cut(s)	177, 711, 933, 1404, 1929, 2292
SacI	GAGCTC	7 cut(s)	962, 2122, 2891, 2909, 3037, 3691, 3998
SapI	GCTCTTC	1 cut(s)	93
SchI	GAGTC	8 cut(s)	1165, 1367, 1729, 1928, 2872, 3638, 4243, 4820
SfcI	CTRYAG	4 cut(s)	1440, 2386, 3465, 4904
SinI	GGWCC	5 cut(s)	266, 1226, 1798, 3027, 3702
SmiMI	CAYNNNNRTG	6 cut(s)	177, 711, 933, 1404, 1929, 2292
SmlI	CTYRAG	4 cut(s)	136, 1164, 1234, 3755
SmoI	CTYRAG	4 cut(s)	136, 1164, 1234, 3755
SnaBI	TACGTA	1 cut(s)	1420
SpeI	ACTAGT	1 cut(s)	3239
SphI	GCATGC	1 cut(s)	364
SseBI	AGGCCT	1 cut(s)	1093
SspI	AATATT	3 cut(s)	828, 2317, 2614
SstI	GAGCTC	7 cut(s)	962, 2122, 2891, 2909, 3037, 3691, 3998
StuI	AGGCCT	1 cut(s)	1093
StyI	CCWWGG	7 cut(s)	1913, 3314, 3705, 3779, 4091, 4442, 4502
TaiI	ACGT	4 cut(s)	1422, 1663, 2480, 4454
TaqI	TCGA	7 cut(s)	805, 2682, 3037, 3154, 3398, 3627, 4735
TaqII	GACCGA	1 cut(s)	2246
TatI	WGTACW	5 cut(s)	286, 485, 2093, 2310, 4464
TauI	GCSGC	2 cut(s)	3052, 3594
TspGWI	ACGGA	5 cut(s)	64, 1261, 2446, 2640, 4846
Tth111I	GACNNNGTC	2 cut(s)	270, 3700
Van91I	CCANNNNNTGG	2 cut(s)	2265, 4172
VneI	GTGCAC	1 cut(s)	714
VpaK11BI	GGWCC	5 cut(s)	266, 1226, 1798, 3027, 3702
VspI	ATTAAT	1 cut(s)	4632
XagI	CCTNNNNNAGG	1 cut(s)	3344
XbaI	TCTAGA	3 cut(s)	1448, 2179, 4852
XceI	RCATGY	4 cut(s)	364, 634, 2935, 3919
XcmI	CCANNNNNNNNNTGG	3 cut(s)	970, 1808, 4440
XmaJI	CCTAGG	1 cut(s)	3779
XmiI	GTMKAC	1 cut(s)	3785
ZraI	GACGTC	1 cut(s)	2478
Zsp2I	ATGCAT	5 cut(s)	252, 366, 1783, 2289, 3921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.