MD13G1160200.v1.1

protein DEHYDRATION-INDUCED 19 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008255
Physical Location & Seq
Forward (+)
12591020 .. 12592096
1077 bp
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UTR
Exon/CDS
Intron
MD13G1160200.v1.1.491

Sequence Viewer

Length: 384 bp
ATGTGCTCCTTGACTATACATTACGTTCAGCGCAAGAGGAAATTTCGCAGGGGATCTAGTTCAACATTTTCTATGTTAAGGAAAGAGCTGCGAGAAGGAAGTTTACAATCCCTTCTAGGAGGGTCTTCATTCCTTTCCTCAAACACTGAAGCTGATCCTTTGTTGTCTTCTTTTATATTTAACCCTCCCACTGCTGATGAGGATGTGAGTACAAAGCCGCATCCTTTAGTTGAACCATCTTTTGTAAAGGAGAGCACAAAGGAAGAGGTCTCAGAAAGAAGCGTTCAACAGCCACCCTTATCTCGCAAGGACCAAGAGGAGCAGGCACGGAGGTGTGAGTTTGTTCAAGGATTGCTCATGTCGACTATTCTTGATGATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Di19_C zf-Di19

Protein Analysis

128

Amino Acids

14.37

Weight (kDa)

6.13

Isoelectric Point (pI)

73.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Di19_C PF14571 23 - 123 5.7e-38 Stress-induced protein Di19, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 362
AciI CCGC 1 cut(s) 218
AclWI GGATC 2 cut(s) 61, 149
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 211
AgsI TTSAA 4 cut(s) 63, 233, 287, 347
AleI CACNNNNGTG 1 cut(s) 331
AluBI AGCT 2 cut(s) 88, 152
AluI AGCT 2 cut(s) 88, 152
Alw21I GWGCWC 2 cut(s) 8, 257
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 2 cut(s) 61, 149
ApeKI GCWGC 1 cut(s) 88
ApoI RAATTY 1 cut(s) 41
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 310
AvaII GGWCC 1 cut(s) 310
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BbsI GAAGAC 2 cut(s) 117, 159
Bbv12I GWGCWC 2 cut(s) 8, 257
BbvI GCAGC 1 cut(s) 75
BccI CCATC 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 2 cut(s) 57, 116
BisI GCNGC 2 cut(s) 89, 218
BlsI GCNGC 2 cut(s) 90, 219
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 2 cut(s) 117, 159
BsaI GGTCTC 1 cut(s) 274
BseGI GGATG 2 cut(s) 208, 220
BseMII CTCAG 1 cut(s) 285
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 8, 257
BsmAI GTCTC 1 cut(s) 274
Bso31I GGTCTC 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 8, 257
Bsp143I GATC 2 cut(s) 53, 154
BspACI CCGC 1 cut(s) 218
BspCNI CTCAG 1 cut(s) 284
BspPI GGATC 2 cut(s) 61, 149
BspTNI GGTCTC 1 cut(s) 274
BssMI GATC 2 cut(s) 53, 154
Bst6I CTCTTC 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 208, 220
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 56, 157
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 2 cut(s) 53, 154
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 2 cut(s) 117, 159
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BtsCI GGATG 2 cut(s) 208, 220
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 2 cut(s) 144, 189
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 210
CviAII CATG 1 cut(s) 358
CviJI RGCY 4 cut(s) 88, 152, 217, 292
CviKI_1 RGCY 4 cut(s) 88, 152, 217, 292
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 1 cut(s) 271
DpnI GATC 2 cut(s) 55, 156
DpnII GATC 2 cut(s) 53, 154
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 168
FaeI CATG 1 cut(s) 361
FaiI YATR 5 cut(s) 17, 74, 176, 359, 382
FatI CATG 1 cut(s) 357
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 2 cut(s) 89, 218
FokI GGATG 2 cut(s) 207, 215
Fsp4HI GCNGC 2 cut(s) 89, 218
FspBI CTAG 2 cut(s) 57, 116
GlaI GCGC 1 cut(s) 32
GluI GCNGC 2 cut(s) 89, 218
HhaI GCGC 1 cut(s) 33
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
Hpy166II GTNNAC 2 cut(s) 104, 363
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 2 cut(s) 104, 363
HpyAV CCTTC 2 cut(s) 89, 122
HpyCH4IV ACGT 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 53, 154
LmnI GCTCC 2 cut(s) 11, 319
LpnPI CCDG 2 cut(s) 34, 308
Lsp1109I GCAGC 1 cut(s) 75
LweI GCATC 1 cut(s) 229
MaeI CTAG 2 cut(s) 57, 116
MaeII ACGT 1 cut(s) 24
MalI GATC 2 cut(s) 55, 156
MboI GATC 2 cut(s) 53, 154
MboII GAAGA 3 cut(s) 117, 159, 275
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 2 cut(s) 8, 257
MluCI AATT 1 cut(s) 41
MnlI CCTC 8 cut(s) 30, 113, 148, 193, 195, 259, 310, 324
MseI TTAA 2 cut(s) 77, 180
MslI CAYNNNNRTG 1 cut(s) 331
NdeII GATC 2 cut(s) 53, 154
NlaIII CATG 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 331
PkrI GCNGC 2 cut(s) 90, 219
PspPI GGNCC 1 cut(s) 310
PsuI RGATCY 1 cut(s) 53
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
RseI CAYNNNNRTG 1 cut(s) 331
SalI GTCGAC 1 cut(s) 361
SaqAI TTAA 2 cut(s) 77, 180
SatI GCNGC 2 cut(s) 89, 218
Sau3AI GATC 2 cut(s) 53, 154
Sau96I GGNCC 1 cut(s) 310
SduI GDGCHC 2 cut(s) 8, 257
SetI ASST 5 cut(s) 27, 90, 154, 270, 335
SfaNI GCATC 1 cut(s) 229
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 331
Sse9I AATT 1 cut(s) 41
SsiI CCGC 1 cut(s) 218
SspMI CTAG 2 cut(s) 57, 116
TaiI ACGT 1 cut(s) 27
TaqI TCGA 1 cut(s) 362
TasI AATT 1 cut(s) 41
TatI WGTACW 1 cut(s) 209
TauI GCSGC 1 cut(s) 220
Tru1I TTAA 2 cut(s) 77, 180
Tru9I TTAA 2 cut(s) 77, 180
TscAI CASTG 2 cut(s) 151, 196
TseI GCWGC 1 cut(s) 88
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 2 cut(s) 151, 196
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 362
XspI CTAG 2 cut(s) 57, 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.