MD13G1160300.v1.1

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009360
Physical Location & Seq
Reverse (-)
12593526 .. 12595858
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1160300.v1.1.491

Sequence Viewer

Length: 945 bp
ATGATAGTAACTAGTTTGAACTGCATTTTCCTTTCTTTAGTGAAACATGCGAGTCCTGGTTCCAAACAATTACCTACCTTTGTCATCTATGAGAGAAATTGTCTATCAGCTGTGAGTTTGTGCACAGAGGTACACAAACTCAATGTTCACTCAGATGAAATCAATCTGTTGGCTAGTAACTGTTTGTATAACATAATTTTCCACTCGTGTGCATGTTATTACTGGAGGCCTGGTGATGAGAGTGATGCTGAGTCATCCAGGGAGACAAGTAGTGATGGCAGCAGTGATTATGGAACAGAAAGAGGATTCAACAATGTTCCTTATGGTACTTTAGGTTGGCAGAATGCTACAGATGTAAATGGCCATGGTTGGAATAAAACCGTACCGAGAACTGAACCCTTAAATGGTTCCTCAAGTGATGAAAGTGGTGAGGCTTGCAACCCTCCTGGTCAACTTATCTTTCAATATATGGAGCATGATCAACCATTTGGTCGTGAACCTTTGGCTGATAAGATCTCATTTCTTGCATCTCAATTTCCAGAGCTGAGGACTTACAGGAGCTGTGATTTATCACCTTCTAGTTGGATTTCTGTAGCATGGTATCCAATATATAGGATACCTACAGGTCCAACTTTGCAGAGCCTCGATGCCTGCTTCTTGACCTTTCATTTCGTATCAACTCCCACAAAGGGTGCAAGCACTGATAGGCTTCAGTTTGGTGGTCCACGAGCTAGAGACAATCAAAGTGCCAATTTCAAACTATCACTGCCAATCTTTGGGCTTGCTTCGTACAAGTTCAAAGTTCCCTTTTGGAATTCTAATGGAGTTTATGAATGTCAAAAGGCTGATTCTCTATTGCGAGCAGCTGACAACTGGCTCAGGCAATTGCAAGTTCATCATCCTGATTTCAATTTCTTTGTGTCGCACAGCATTCCAAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

315

Amino Acids

35.22

Weight (kDa)

6.19

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 76 - 306 7.5e-69 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 695
AfaI GTAC 4 cut(s) 132, 328, 384, 791
AfiI CCNNNNNNNGG 3 cut(s) 404, 689, 776
AgsI TTSAA 6 cut(s) 19, 310, 464, 757, 799, 910
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 4 cut(s) 55, 229, 257, 445
AleI CACNNNNGTG 1 cut(s) 207
AluBI AGCT 5 cut(s) 110, 544, 561, 731, 866
AluI AGCT 5 cut(s) 110, 544, 561, 731, 866
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 2 cut(s) 257, 729
Alw44I GTGCAC 1 cut(s) 121
AlwNI CAGNNNCTG 1 cut(s) 561
AoxI GGCC 2 cut(s) 227, 361
ApaLI GTGCAC 1 cut(s) 121
ApeKI GCWGC 2 cut(s) 279, 863
ApoI RAATTY 1 cut(s) 814
AspS9I GGNCC 2 cut(s) 626, 722
AsuHPI GGTGA 3 cut(s) 245, 440, 564
AvaII GGWCC 2 cut(s) 626, 722
BaeGI GKGCMC 1 cut(s) 125
BalI TGGCCA 1 cut(s) 363
BauI CACGAG 2 cut(s) 205, 726
Bbv12I GWGCWC 1 cut(s) 125
BbvCI CCTCAGC 1 cut(s) 545
BbvI GCAGC 2 cut(s) 291, 875
BccI CCATC 1 cut(s) 269
BciT130I CCWGG 4 cut(s) 57, 231, 259, 447
BciVI GTATCC 2 cut(s) 609, 612
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 257, 729
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 4 cut(s) 12, 174, 579, 732
BfmI CTRYAG 3 cut(s) 348, 591, 621
BfuI GTATCC 2 cut(s) 609, 612
BglII AGATCT 1 cut(s) 513
BisI GCNGC 2 cut(s) 280, 864
BlsI GCNGC 2 cut(s) 281, 865
Bme1390I CCNGG 4 cut(s) 57, 231, 259, 447
Bme18I GGWCC 2 cut(s) 626, 722
BmgT120I GGNCC 2 cut(s) 626, 722
BmiI GGNNCC 2 cut(s) 61, 409
BmrFI CCNGG 4 cut(s) 57, 231, 259, 447
BmsI GCATC 3 cut(s) 235, 536, 637
BpmI CTGGAG 1 cut(s) 244
Bpu10I CCTNAGC 2 cut(s) 545, 878
BpuEI CTTGAG 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 258, 364
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 689, 776
Bse1I ACTGG 2 cut(s) 227, 878
BseBI CCWGG 4 cut(s) 57, 231, 259, 447
BseDI CCNNGG 2 cut(s) 258, 364
BseGI GGATG 2 cut(s) 254, 898
BseLI CCNNNNNNNGG 3 cut(s) 404, 689, 776
BseMII CTCAG 4 cut(s) 165, 240, 536, 892
BseNI ACTGG 2 cut(s) 227, 878
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 2 cut(s) 291, 875
BshFI GGCC 2 cut(s) 229, 363
BsiHKAI GWGCWC 1 cut(s) 125
BslI CCNNNNNNNGG 3 cut(s) 404, 689, 776
BsmAI GTCTC 2 cut(s) 257, 729
BsmI GAATGC 2 cut(s) 349, 930
BsnI GGCC 2 cut(s) 229, 363
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 478, 513
Bsp19I CCATGG 1 cut(s) 364
BspANI GGCC 2 cut(s) 229, 363
BspCNI CTCAG 4 cut(s) 164, 241, 537, 891
BspLI GGNNCC 2 cut(s) 61, 409
BsrI ACTGG 2 cut(s) 227, 878
BssECI CCNNGG 2 cut(s) 258, 364
BssMI GATC 2 cut(s) 478, 513
BssSI CACGAG 2 cut(s) 205, 726
BssT1I CCWWGG 1 cut(s) 364
Bst2BI CACGAG 2 cut(s) 205, 726
Bst2UI CCWGG 4 cut(s) 57, 231, 259, 447
Bst4CI ACNGT 2 cut(s) 182, 382
BstC8I GCNNGC 5 cut(s) 436, 652, 697, 783, 861
BstDEI CTNAG 4 cut(s) 151, 249, 545, 878
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 2 cut(s) 254, 898
BstKTI GATC 2 cut(s) 481, 516
BstMAI GTCTC 2 cut(s) 257, 729
BstMBI GATC 2 cut(s) 478, 513
BstNI CCWGG 4 cut(s) 57, 231, 259, 447
BstNSI RCATGY 2 cut(s) 50, 216
BstSCI CCNGG 4 cut(s) 55, 229, 257, 445
BstSFI CTRYAG 3 cut(s) 348, 591, 621
BstSLI GKGCMC 1 cut(s) 125
BstV1I GCAGC 2 cut(s) 291, 875
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
BsuI GTATCC 2 cut(s) 609, 612
BsuRI GGCC 2 cut(s) 229, 363
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 2 cut(s) 254, 898
BtsI GCAGTG 2 cut(s) 289, 764
BtsIMutI CAGTG 3 cut(s) 289, 699, 764
Cac8I GCNNGC 5 cut(s) 436, 652, 697, 783, 861
CaiI CAGNNNCTG 1 cut(s) 561
Cfr13I GGNCC 2 cut(s) 626, 722
Csp6I GTAC 4 cut(s) 131, 327, 383, 790
CviAII CATG 5 cut(s) 47, 213, 365, 476, 597
CviQI GTAC 4 cut(s) 131, 327, 383, 790
DdeI CTNAG 4 cut(s) 151, 249, 545, 878
DpnI GATC 2 cut(s) 480, 515
DpnII GATC 2 cut(s) 478, 513
EaeI YGGCCR 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 626, 722
Eco57I CTGAAG 1 cut(s) 695
EcoRI GAATTC 1 cut(s) 814
EcoRII CCWGG 4 cut(s) 55, 229, 257, 445
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 5 cut(s) 50, 216, 368, 479, 600
FatI CATG 5 cut(s) 46, 212, 364, 475, 596
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 2 cut(s) 280, 864
FokI GGATG 2 cut(s) 241, 885
Fsp4HI GCNGC 2 cut(s) 280, 864
FspBI CTAG 4 cut(s) 12, 174, 579, 732
GluI GCNGC 2 cut(s) 280, 864
GsuI CTGGAG 1 cut(s) 244
HaeIII GGCC 2 cut(s) 229, 363
Hin1II CATG 5 cut(s) 50, 216, 368, 479, 600
HincII GTYRAC 1 cut(s) 452
HindII GTYRAC 1 cut(s) 452
HinfI GANTC 4 cut(s) 52, 251, 306, 848
HphI GGTGA 3 cut(s) 245, 440, 564
Hpy166II GTNNAC 6 cut(s) 123, 133, 148, 452, 497, 725
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 4 cut(s) 494, 539, 658, 902
Hpy8I GTNNAC 6 cut(s) 123, 133, 148, 452, 497, 725
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 2 cut(s) 182, 382
HpyCH4V TGCA 8 cut(s) 24, 123, 212, 438, 527, 637, 695, 889
HpyF3I CTNAG 4 cut(s) 151, 249, 545, 878
Hsp92II CATG 5 cut(s) 50, 216, 368, 479, 600
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 2 cut(s) 478, 513
LmnI GCTCC 2 cut(s) 472, 558
Lsp1109I GCAGC 2 cut(s) 291, 875
LweI GCATC 3 cut(s) 235, 536, 637
MaeI CTAG 4 cut(s) 12, 174, 579, 732
MaeIII GTNAC 2 cut(s) 7, 176
MalI GATC 2 cut(s) 480, 515
MboI GATC 2 cut(s) 478, 513
MfeI CAATTG 1 cut(s) 884
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 1 cut(s) 125
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 8 cut(s) 68, 97, 195, 533, 751, 814, 884, 910
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 2 cut(s) 61, 260
MmeI TCCRAC 3 cut(s) 350, 563, 653
MnlI CCTC 9 cut(s) 121, 219, 296, 421, 424, 453, 540, 653, 933
Mox20I TGGCCA 1 cut(s) 363
MscI TGGCCA 1 cut(s) 363
MseI TTAA 1 cut(s) 401
MslI CAYNNNNRTG 2 cut(s) 153, 207
Msp20I TGGCCA 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 110, 866
MspR9I CCNGG 4 cut(s) 57, 231, 259, 447
MunI CAATTG 1 cut(s) 884
Mva1269I GAATGC 2 cut(s) 349, 930
MvaI CCWGG 4 cut(s) 57, 231, 259, 447
NcoI CCATGG 1 cut(s) 364
NdeII GATC 2 cut(s) 478, 513
NlaIII CATG 5 cut(s) 50, 216, 368, 479, 600
NlaIV GGNNCC 2 cut(s) 61, 409
NspI RCATGY 2 cut(s) 50, 216
OliI CACNNNNGTG 1 cut(s) 207
PceI AGGCCT 1 cut(s) 229
PctI GAATGC 2 cut(s) 349, 930
PfeI GAWTC 2 cut(s) 306, 848
PflMI CCANNNNNTGG 1 cut(s) 776
PkrI GCNGC 2 cut(s) 281, 865
PleI GAGTC 2 cut(s) 60, 259
PpsI GAGTC 2 cut(s) 60, 259
Psp6I CCWGG 4 cut(s) 55, 229, 257, 445
PspGI CCWGG 4 cut(s) 55, 229, 257, 445
PspN4I GGNNCC 2 cut(s) 61, 409
PspPI GGNCC 2 cut(s) 626, 722
PstNI CAGNNNCTG 1 cut(s) 561
PsuI RGATCY 1 cut(s) 513
PvuII CAGCTG 2 cut(s) 110, 866
RsaI GTAC 4 cut(s) 132, 328, 384, 791
RsaNI GTAC 4 cut(s) 131, 327, 383, 790
RseI CAYNNNNRTG 2 cut(s) 153, 207
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 2 cut(s) 280, 864
Sau3AI GATC 2 cut(s) 478, 513
Sau96I GGNCC 2 cut(s) 626, 722
SchI GAGTC 2 cut(s) 61, 260
ScrFI CCNGG 4 cut(s) 57, 231, 259, 447
SduI GDGCHC 1 cut(s) 125
SfaNI GCATC 3 cut(s) 235, 536, 637
SfcI CTRYAG 3 cut(s) 348, 591, 621
SinI GGWCC 2 cut(s) 626, 722
SmiMI CAYNNNNRTG 2 cut(s) 153, 207
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 8 cut(s) 68, 97, 195, 533, 751, 814, 884, 910
SseBI AGGCCT 1 cut(s) 229
SspMI CTAG 4 cut(s) 12, 174, 579, 732
StuI AGGCCT 1 cut(s) 229
StyD4I CCNGG 4 cut(s) 55, 229, 257, 445
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 182, 382
TaqI TCGA 1 cut(s) 645
TasI AATT 8 cut(s) 68, 97, 195, 533, 751, 814, 884, 910
TfiI GAWTC 2 cut(s) 306, 848
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TscAI CASTG 3 cut(s) 289, 706, 771
TseI GCWGC 2 cut(s) 279, 863
TspDTI ATGAA 5 cut(s) 171, 435, 656, 846, 884
TspRI CASTG 3 cut(s) 289, 706, 771
Van91I CCANNNNNTGG 1 cut(s) 776
VneI GTGCAC 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 626, 722
XapI RAATTY 1 cut(s) 814
XceI RCATGY 2 cut(s) 50, 216
XspI CTAG 4 cut(s) 12, 174, 579, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.