RchiOBHm_Chr4g0424141

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009360
Physical Location & Seq
Forward (+)
49715651 .. 49719714
4064 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39345

Sequence Viewer

Length: 1260 bp
ATGTCTGGTCACGGCGGAGTTTCGATCGGTCGGGCTCGGGGCGAGAACCGGTTCTACAACCCGCCCCCGATTCGAGAGCGACAACACCAGCAACAGCTTCAGCAGCAGCAGCAGCAGAAGCTTGAACAGGAGAAGCAGCAGCATCAGCATCAGAGGCGGCCGGTGTCGGTGTCGGTGTCGGTGTCGGTGTCGGTGTCGGTGGTCGAGTCCGACGACTGTGCTACGTCGCATAGTTCCGGGTCGGGTCGGGAAGCGCTCAGCGAGGCCGCGAATTTAGATCGGTTCCTTGAGTTCACAACGCCCCTGGCTCGAGCTCATTTTTTGCCCAAGACGAGTAGCAGGGGATGGAGGACTCATGAGCCGGAGTTACATCCCTACTTTGTGCTTGGAGATTTGTGGGAATCTTTCAAGGAGTGGAGTGCGTACGGCGCGGGTGTCCCTCTTTTGTTAAATGGGAGTGACTCTGTCATACAGTACTATGTACCTTACTTGTCTGCCATTCAGCTCTATGTAGACCCATCAAAGCCTCCCACAAGGATAAGGAAGCCTGGTGATGACAGTGATGCAGAGTCATCGAGGGAGACAAGTAGTGATGGCAGCAGTGATTATGGAGCAGAAAGAGGAATCAGTGGTGTTGTTGATGATGCTTGGAGTCGGCAGAATATTGCTGATGTAAATGGCCATGGTTGGAATAGAGTCTTACCAAGAGATAAACCCTTAACTGGTTCCTCAAGTGATGAAAGTGAGATTTGCAATCCTCCAGGTCAGCTTGTATTTCAATATTTGGAGCACGATCAACCGTTTGGCCGTGAACCTTTGGCTGATAAGATTCAAGTTCTTGCATCCCAATTTCCAGAGTTGAGGACTTACAGGAGCTCGGACTTATCACCTTCTAGTTGGATCTCTGTAGCATGGTATCCTATATATCGGATACCTACAGGTCCAACATTACAGAGTCTGGATGCTTGCTTCTTGACCTTTCATTCCCTTTCGACTCCCTTAAAGGGTACAAGCACTGATAGGCTTCGTCCCAGCGGTGCACGACCTAGAGAGGTTCAAAATGCCGACTTCAAACTCTCGTTGCCAACTTTTGGATTGGCTTCCTACAAGTTCAAGGGTTCCTTTTGGAACCCTAATGGAGTTTACGAATCTCAAAAGGCTAATTATCTATTGCGAGCAGCTGAAAACTGGATCAGGCTATTGCAAGTTCATCATCCTGATTTCAACTTCTTTGTGTCACACAATACACCAAGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

419

Amino Acids

47.13

Weight (kDa)

6.71

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 90 - 411 8.7e-105 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccI GTMKAC 1 cut(s) 513
AccII CGCG 2 cut(s) 269, 431
AciI CCGC 6 cut(s) 15, 62, 157, 267, 431, 1035
AclWI GGATC 2 cut(s) 908, 1199
AcoI YGGCCR 3 cut(s) 158, 679, 805
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 4 cut(s) 425, 476, 483, 1009
AfeI AGCGCT 1 cut(s) 255
AfiI CCNNNNNNNGG 3 cut(s) 722, 1004, 1091
AgeI ACCGGT 1 cut(s) 48
AgsI TTSAA 8 cut(s) 125, 409, 779, 833, 1058, 1072, 1114, 1225
AjnI CCWGG 3 cut(s) 303, 547, 760
AluBI AGCT 7 cut(s) 97, 121, 314, 505, 769, 876, 1181
AluI AGCT 7 cut(s) 97, 121, 314, 505, 769, 876, 1181
Alw21I GWGCWC 4 cut(s) 316, 792, 878, 1042
Alw26I GTCTC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 1038
AlwI GGATC 2 cut(s) 908, 1199
AlwNI CAGNNNCTG 1 cut(s) 958
Ama87I CYCGRG 2 cut(s) 36, 309
Aor51HI AGCGCT 1 cut(s) 255
AoxI GGCC 4 cut(s) 158, 264, 679, 805
ApaLI GTGCAC 1 cut(s) 1038
ApeKI GCWGC 8 cut(s) 103, 106, 109, 112, 136, 139, 597, 1178
ApoI RAATTY 1 cut(s) 271
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 2 cut(s) 256, 431
AspS9I GGNCC 1 cut(s) 941
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 2 cut(s) 563, 879
AvaI CYCGRG 2 cut(s) 36, 309
AvaII GGWCC 1 cut(s) 941
BaeGI GKGCMC 1 cut(s) 1042
BalI TGGCCA 1 cut(s) 681
BanII GRGCYC 3 cut(s) 37, 316, 878
Bbv12I GWGCWC 4 cut(s) 316, 792, 878, 1042
BbvI GCAGC 8 cut(s) 115, 118, 121, 124, 148, 151, 609, 1190
BccI CCATC 3 cut(s) 339, 526, 587
BceAI ACGGC 3 cut(s) 28, 442, 792
BcgI CGANNNNNNTGC 4 cut(s) 200, 234, 555, 589
BciT130I CCWGG 3 cut(s) 305, 549, 762
BciVI GTATCC 2 cut(s) 924, 927
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 2 cut(s) 894, 1047
BfmI CTRYAG 2 cut(s) 906, 936
BfoI RGCGCY 1 cut(s) 257
BfuI GTATCC 2 cut(s) 924, 927
BlpI GCTNAGC 1 cut(s) 257
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 4 cut(s) 238, 305, 549, 762
Bme18I GGWCC 1 cut(s) 941
BmeT110I CYCGRG 2 cut(s) 36, 309
BmgT120I GGNCC 1 cut(s) 941
BmiI GGNNCC 4 cut(s) 284, 727, 1120, 1130
BmrFI CCNGG 4 cut(s) 238, 305, 549, 762
BmsI GCATC 6 cut(s) 151, 157, 553, 634, 851, 952
BpmI CTGGAG 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 308, 715
BpuMI CCSGG 1 cut(s) 238
BsaJI CCNNGG 3 cut(s) 303, 682, 1250
BsaWI WCCGGW 1 cut(s) 48
Bsc4I CCNNNNNNNGG 3 cut(s) 722, 1004, 1091
Bse118I RCCGGY 2 cut(s) 48, 160
Bse1I ACTGG 2 cut(s) 727, 1193
BseBI CCWGG 3 cut(s) 305, 549, 762
BseDI CCNNGG 3 cut(s) 303, 682, 1250
BseGI GGATG 5 cut(s) 350, 370, 842, 967, 1213
BseLI CCNNNNNNNGG 3 cut(s) 722, 1004, 1091
BseMII CTCAG 1 cut(s) 271
BseNI ACTGG 2 cut(s) 727, 1193
BseSI GKGCMC 1 cut(s) 1042
BseX3I CGGCCG 1 cut(s) 158
BseXI GCAGC 8 cut(s) 115, 118, 121, 124, 148, 151, 609, 1190
BseYI CCCAGC 1 cut(s) 1031
Bsh1236I CGCG 2 cut(s) 269, 431
Bsh1285I CGRYCG 3 cut(s) 27, 31, 161
BshFI GGCC 4 cut(s) 160, 266, 681, 807
BshTI ACCGGT 1 cut(s) 48
BsiEI CGRYCG 3 cut(s) 27, 31, 161
BsiHKAI GWGCWC 4 cut(s) 316, 792, 878, 1042
BsiHKCI CYCGRG 2 cut(s) 36, 309
BsiSI CCGG 4 cut(s) 49, 161, 237, 362
BsiWI CGTACG 1 cut(s) 423
BslFI GGGAC 2 cut(s) 422, 1014
BslI CCNNNNNNNGG 3 cut(s) 722, 1004, 1091
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 2 cut(s) 422, 1014
BsnI GGCC 4 cut(s) 160, 266, 681, 807
BsoBI CYCGRG 2 cut(s) 36, 309
Bsp1286I GDGCHC 5 cut(s) 37, 316, 792, 878, 1042
Bsp143I GATC 5 cut(s) 24, 277, 793, 900, 1191
Bsp1720I GCTNAGC 1 cut(s) 257
Bsp19I CCATGG 1 cut(s) 682
BspACI CCGC 6 cut(s) 15, 62, 157, 267, 431, 1035
BspANI GGCC 4 cut(s) 160, 266, 681, 807
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 2 cut(s) 269, 431
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 4 cut(s) 284, 727, 1120, 1130
BspPI GGATC 2 cut(s) 908, 1199
BsrFI RCCGGY 2 cut(s) 48, 160
BsrI ACTGG 2 cut(s) 727, 1193
BssAI RCCGGY 2 cut(s) 48, 160
BssECI CCNNGG 3 cut(s) 303, 682, 1250
BssMI GATC 5 cut(s) 24, 277, 793, 900, 1191
BssT1I CCWWGG 2 cut(s) 682, 1250
Bst2UI CCWGG 3 cut(s) 305, 549, 762
Bst4CI ACNGT 4 cut(s) 218, 474, 560, 801
BstC8I GCNNGC 2 cut(s) 967, 1176
BstDEI CTNAG 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 682
BstF5I GGATG 5 cut(s) 350, 370, 842, 967, 1213
BstFNI CGCG 2 cut(s) 269, 431
BstH2I RGCGCY 1 cut(s) 257
BstHHI GCGC 2 cut(s) 256, 431
BstKTI GATC 5 cut(s) 27, 280, 796, 903, 1194
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 5 cut(s) 24, 277, 793, 900, 1191
BstMCI CGRYCG 3 cut(s) 27, 31, 161
BstMWI GCNNNNNNNGC 7 cut(s) 103, 109, 112, 118, 145, 154, 428
BstNI CCWGG 3 cut(s) 305, 549, 762
BstSCI CCNGG 4 cut(s) 236, 303, 547, 760
BstSFI CTRYAG 2 cut(s) 906, 936
BstSLI GKGCMC 1 cut(s) 1042
BstUI CGCG 2 cut(s) 269, 431
BstV1I GCAGC 8 cut(s) 115, 118, 121, 124, 148, 151, 609, 1190
BstX2I RGATCY 1 cut(s) 900
BstYI RGATCY 1 cut(s) 900
BstZI CGGCCG 1 cut(s) 158
BsuI GTATCC 2 cut(s) 924, 927
BsuRI GGCC 4 cut(s) 160, 266, 681, 807
BtgI CCRYGG 1 cut(s) 682
BtsCI GGATG 5 cut(s) 350, 370, 842, 967, 1213
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 4 cut(s) 565, 607, 634, 1014
Cac8I GCNNGC 2 cut(s) 967, 1176
CaiI CAGNNNCTG 1 cut(s) 958
CciI TCATGA 1 cut(s) 355
CfoI GCGC 2 cut(s) 256, 431
Cfr10I RCCGGY 2 cut(s) 48, 160
Cfr13I GGNCC 1 cut(s) 941
Csp6I GTAC 4 cut(s) 424, 475, 482, 1008
CspAI ACCGGT 1 cut(s) 48
CviAII CATG 3 cut(s) 356, 683, 912
CviQI GTAC 4 cut(s) 424, 475, 482, 1008
DdeI CTNAG 1 cut(s) 257
DpnI GATC 5 cut(s) 26, 279, 795, 902, 1193
DpnII GATC 5 cut(s) 24, 277, 793, 900, 1191
EaeI YGGCCR 3 cut(s) 158, 679, 805
EagI CGGCCG 1 cut(s) 158
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 2 cut(s) 314, 876
EclXI CGGCCG 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 682, 1250
Eco24I GRGCYC 3 cut(s) 37, 316, 878
Eco47I GGWCC 1 cut(s) 941
Eco47III AGCGCT 1 cut(s) 255
Eco52I CGGCCG 1 cut(s) 158
Eco53kI GAGCTC 2 cut(s) 314, 876
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 36, 309
EcoICRI GAGCTC 2 cut(s) 314, 876
EcoRII CCWGG 3 cut(s) 303, 547, 760
EcoT14I CCWWGG 2 cut(s) 682, 1250
EcoT38I GRGCYC 3 cut(s) 37, 316, 878
ErhI CCWWGG 2 cut(s) 682, 1250
FaeI CATG 3 cut(s) 359, 686, 915
FalI AAGNNNNNCTT 2 cut(s) 1106, 1138
FaqI GGGAC 2 cut(s) 422, 1014
FatI CATG 3 cut(s) 355, 682, 911
FauI CCCGC 2 cut(s) 69, 424
FblI GTMKAC 1 cut(s) 513
FokI GGATG 5 cut(s) 357, 357, 829, 974, 1200
FriOI GRGCYC 3 cut(s) 37, 316, 878
FspBI CTAG 2 cut(s) 894, 1047
GlaI GCGC 2 cut(s) 255, 430
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 1 cut(s) 744
HaeII RGCGCY 1 cut(s) 257
HaeIII GGCC 4 cut(s) 160, 266, 681, 807
HapII CCGG 4 cut(s) 49, 161, 237, 362
HhaI GCGC 2 cut(s) 256, 431
Hin1II CATG 3 cut(s) 359, 686, 915
Hin6I GCGC 2 cut(s) 254, 429
HinP1I GCGC 2 cut(s) 254, 429
HindIII AAGCTT 1 cut(s) 119
HpaII CCGG 4 cut(s) 49, 161, 237, 362
HphI GGTGA 2 cut(s) 563, 879
Hpy166II GTNNAC 5 cut(s) 294, 514, 812, 1040, 1144
Hpy188I TCNGA 4 cut(s) 153, 211, 880, 930
Hpy188III TCNNGA 7 cut(s) 74, 248, 356, 854, 959, 973, 1217
Hpy8I GTNNAC 5 cut(s) 294, 514, 812, 1040, 1144
Hpy99I CGWCG 2 cut(s) 215, 229
HpyAV CCTTC 1 cut(s) 900
HpyCH4III ACNGT 4 cut(s) 218, 474, 560, 801
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 5 cut(s) 566, 753, 842, 1040, 1204
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 109, 112, 118, 145, 154, 428
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 3 cut(s) 359, 686, 915
HspAI GCGC 2 cut(s) 254, 429
Kzo9I GATC 5 cut(s) 24, 277, 793, 900, 1191
LmnI GCTCC 3 cut(s) 611, 787, 873
Lsp1109I GCAGC 8 cut(s) 115, 118, 121, 124, 148, 151, 609, 1190
LweI GCATC 6 cut(s) 151, 157, 553, 634, 851, 952
MaeI CTAG 2 cut(s) 894, 1047
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 4 cut(s) 8, 366, 458, 1236
MalI GATC 5 cut(s) 26, 279, 795, 902, 1193
MboI GATC 5 cut(s) 24, 277, 793, 900, 1191
MflI RGATCY 1 cut(s) 900
MhlI GDGCHC 5 cut(s) 37, 316, 792, 878, 1042
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 3 cut(s) 271, 848, 1162
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 8 cut(s) 215, 346, 455, 578, 661, 705, 964, 988
MmeI TCCRAC 4 cut(s) 234, 668, 878, 968
Mox20I TGGCCA 1 cut(s) 681
MroXI GAANNNNTTC 1 cut(s) 50
MscI TGGCCA 1 cut(s) 681
MseI TTAA 3 cut(s) 449, 719, 1001
Msp20I TGGCCA 1 cut(s) 681
MspA1I CMGCKG 2 cut(s) 1035, 1181
MspI CCGG 4 cut(s) 49, 161, 237, 362
MspR9I CCNGG 4 cut(s) 238, 305, 549, 762
MvaI CCWGG 3 cut(s) 305, 549, 762
MvnI CGCG 2 cut(s) 269, 431
MwoI GCNNNNNNNGC 7 cut(s) 103, 109, 112, 118, 145, 154, 428
NciI CCSGG 1 cut(s) 238
NcoI CCATGG 1 cut(s) 682
NdeII GATC 5 cut(s) 24, 277, 793, 900, 1191
NlaIII CATG 3 cut(s) 359, 686, 915
NlaIV GGNNCC 4 cut(s) 284, 727, 1120, 1130
NmuCI GTSAC 3 cut(s) 8, 458, 1236
PaeR7I CTCGAG 1 cut(s) 309
PagI TCATGA 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 5 cut(s) 70, 401, 624, 829, 1148
Pfl23II CGTACG 1 cut(s) 423
PflFI GACNNNGTC 1 cut(s) 464
PflMI CCANNNNNTGG 1 cut(s) 1091
PinAI ACCGGT 1 cut(s) 48
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 8 cut(s) 214, 346, 455, 577, 660, 704, 963, 988
PpsI GAGTC 8 cut(s) 214, 346, 455, 577, 660, 704, 963, 988
Psp124BI GAGCTC 2 cut(s) 316, 878
Psp6I CCWGG 3 cut(s) 303, 547, 760
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 3 cut(s) 303, 547, 760
PspLI CGTACG 1 cut(s) 423
PspN4I GGNNCC 4 cut(s) 284, 727, 1120, 1130
PspPI GGNCC 1 cut(s) 941
PspXI VCTCGAGB 1 cut(s) 309
PsrI GAACNNNNNNTAC 2 cut(s) 38, 70
PstNI CAGNNNCTG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 900
PsyI GACNNNGTC 1 cut(s) 464
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 4 cut(s) 425, 476, 483, 1009
RsaNI GTAC 4 cut(s) 424, 475, 482, 1008
SacI GAGCTC 2 cut(s) 316, 878
SaqAI TTAA 3 cut(s) 449, 719, 1001
Sau3AI GATC 5 cut(s) 24, 277, 793, 900, 1191
Sau96I GGNCC 1 cut(s) 941
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 8 cut(s) 215, 346, 455, 578, 661, 705, 964, 988
ScrFI CCNGG 4 cut(s) 238, 305, 549, 762
SduI GDGCHC 5 cut(s) 37, 316, 792, 878, 1042
SfaNI GCATC 6 cut(s) 151, 157, 553, 634, 851, 952
SfcI CTRYAG 2 cut(s) 906, 936
Sfr274I CTCGAG 1 cut(s) 309
SinI GGWCC 1 cut(s) 941
SlaI CTCGAG 1 cut(s) 309
SmlI CTYRAG 3 cut(s) 287, 309, 730
SmoI CTYRAG 3 cut(s) 287, 309, 730
Sse9I AATT 3 cut(s) 271, 848, 1162
SsiI CCGC 6 cut(s) 15, 62, 157, 267, 431, 1035
SspI AATATT 2 cut(s) 664, 782
SspMI CTAG 2 cut(s) 894, 1047
SstI GAGCTC 2 cut(s) 316, 878
StyD4I CCNGG 4 cut(s) 236, 303, 547, 760
StyI CCWWGG 2 cut(s) 682, 1250
TaaI ACNGT 4 cut(s) 218, 474, 560, 801
TaiI ACGT 1 cut(s) 227
TaqI TCGA 6 cut(s) 23, 73, 204, 310, 575, 992
TaqII GACCGA 1 cut(s) 17
TasI AATT 3 cut(s) 271, 848, 1162
TatI WGTACW 1 cut(s) 474
TauI GCSGC 2 cut(s) 160, 269
TfiI GAWTC 5 cut(s) 70, 401, 624, 829, 1148
Tru1I TTAA 3 cut(s) 449, 719, 1001
Tru9I TTAA 3 cut(s) 449, 719, 1001
TscAI CASTG 4 cut(s) 565, 607, 634, 1021
TseFI GTSAC 3 cut(s) 8, 458, 1236
TseI GCWGC 8 cut(s) 103, 106, 109, 112, 136, 139, 597, 1178
Tsp45I GTSAC 3 cut(s) 8, 458, 1236
TspDTI ATGAA 3 cut(s) 753, 971, 1199
TspRI CASTG 4 cut(s) 565, 607, 634, 1021
Tth111I GACNNNGTC 1 cut(s) 464
Van91I CCANNNNNTGG 1 cut(s) 1091
VneI GTGCAC 1 cut(s) 1038
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 1 cut(s) 271
XhoI CTCGAG 1 cut(s) 309
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 2 cut(s) 894, 1047
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.