Rh4DG251000

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009360
Physical Location & Seq
Forward (+)
46590029 .. 46594095
4067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG251000.1

Sequence Viewer

Length: 969 bp
ATGTCTGGTCACGGCGGAGTTTCGATCGGTCGGGCTCGGGGCGAGAACCGGTTCTACAACCCGCCCCCGATTCGAGAGCGACAACACCAGCAACTGCTTCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAAGCTTGAACAGGAGAAGCAGCAGCATCAGAGGCGGCCGGTGTCGGTGTCGGTGGTGGAGTCCGACGACTGTGCTACGTCGCATAGTTCCGGGTCGGGTCGGGAAGCGCTCAGCGAGGCCGCGAATTTAGATCGGTTCCTTGAGTTCACAACGCCCCTGGCTCGAGCTCATTTTTTGCCCAAGACGAGTAGCAGGGGATGGAGGACTCATGCGCCGGAGTTACATCCCTACTTTGTGCTTGGAGATTTGTGGGAATCTTTCAAGGAGTGGAGTGCGTACGGCGCGGGCGTCCCTCTTTTGTTAAATGGGAGTGACTCTGTCATACAGTACTATGTACCTTACTTGTCTGCCATTCAGCTCTATGTAGACCCATCAAAGCCTCCCACAAGGATAAGGAAGCCTGGTGATGACAGTGATGCAGAGTCATCAAGGGAGACAAGTAGTGATGGCAGCAGTGATTATGGAGCAGAAAGAGGAATGAATGGTGTTGTTGATGATGCTTGGAGTCGGCAGAATATTGCTGATGTAAATGGCCATGGTTGGAATAGAGTCTTACCAAGAGATAAACCCTTAACTGGTTCCTCAAGTGATGAAAGTGAGATTTGCAATCCTCCAGGTCAGCTTGTATTTCAATATTTGGAGCACGATCAACCGTTTGGCCGTGAACCTTTGGCTGATAAGATTCAAGTTCTTGCATCCCAATTTCCAGAGCTGAGGACTTACAGGAGCTCGGACTTATCACCTTCTAGTTGGATCTCTGTAGCATGGTATGCATTTATTTTTGTATTCACTGGTAGTACAAATAAGGGAAGGATGCATATGAGATTACAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

322

Amino Acids

36.26

Weight (kDa)

6.07

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 85 - 303 7.9e-63 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 498
AccII CGCG 2 cut(s) 254, 416
AciI CCGC 5 cut(s) 15, 62, 166, 252, 416
AclWI GGATC 1 cut(s) 893
AcoI YGGCCR 3 cut(s) 167, 664, 790
AcsI RAATTY 1 cut(s) 256
AcuI CTGAAG 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 4 cut(s) 410, 461, 468, 931
AfeI AGCGCT 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 707
AgeI ACCGGT 1 cut(s) 48
AgsI TTSAA 4 cut(s) 140, 394, 764, 818
AjnI CCWGG 3 cut(s) 288, 532, 745
AluBI AGCT 6 cut(s) 136, 299, 490, 754, 844, 861
AluI AGCT 6 cut(s) 136, 299, 490, 754, 844, 861
Alw21I GWGCWC 3 cut(s) 301, 777, 863
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 1 cut(s) 893
AlwNI CAGNNNCTG 1 cut(s) 94
Ama87I CYCGRG 2 cut(s) 36, 294
Aor51HI AGCGCT 1 cut(s) 240
AoxI GGCC 4 cut(s) 167, 249, 664, 790
ApoI RAATTY 1 cut(s) 256
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 3 cut(s) 241, 346, 416
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 548, 864
AvaI CYCGRG 2 cut(s) 36, 294
BalI TGGCCA 1 cut(s) 666
BanII GRGCYC 3 cut(s) 37, 301, 863
Bbv12I GWGCWC 3 cut(s) 301, 777, 863
BbvCI CCTCAGC 1 cut(s) 845
BccI CCATC 3 cut(s) 324, 511, 572
BceAI ACGGC 3 cut(s) 28, 427, 777
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 3 cut(s) 290, 534, 747
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 1 cut(s) 879
BfmI CTRYAG 1 cut(s) 891
BfoI RGCGCY 1 cut(s) 242
BglII AGATCT 1 cut(s) 963
BlpI GCTNAGC 1 cut(s) 242
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 4 cut(s) 223, 290, 534, 747
BmeT110I CYCGRG 2 cut(s) 36, 294
BmiI GGNNCC 2 cut(s) 269, 712
BmrFI CCNGG 4 cut(s) 223, 290, 534, 747
BmsI GCATC 5 cut(s) 166, 538, 619, 836, 936
BpmI CTGGAG 1 cut(s) 729
Bpu10I CCTNAGC 1 cut(s) 845
Bpu1102I GCTNAGC 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 293, 700
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 288, 667
BsaWI WCCGGW 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 707
Bse118I RCCGGY 2 cut(s) 48, 169
Bse1I ACTGG 2 cut(s) 712, 928
BseBI CCWGG 3 cut(s) 290, 534, 747
BseDI CCNNGG 2 cut(s) 288, 667
BseGI GGATG 4 cut(s) 335, 355, 827, 951
BseLI CCNNNNNNNGG 1 cut(s) 707
BseMII CTCAG 2 cut(s) 256, 836
BseNI ACTGG 2 cut(s) 712, 928
BseX3I CGGCCG 1 cut(s) 167
Bsh1236I CGCG 2 cut(s) 254, 416
Bsh1285I CGRYCG 3 cut(s) 27, 31, 170
BshFI GGCC 4 cut(s) 169, 251, 666, 792
BshTI ACCGGT 1 cut(s) 48
BsiEI CGRYCG 3 cut(s) 27, 31, 170
BsiHKAI GWGCWC 3 cut(s) 301, 777, 863
BsiHKCI CYCGRG 2 cut(s) 36, 294
BsiSI CCGG 4 cut(s) 49, 170, 222, 347
BsiWI CGTACG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 707
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 4 cut(s) 169, 251, 666, 792
BsoBI CYCGRG 2 cut(s) 36, 294
Bsp1286I GDGCHC 4 cut(s) 37, 301, 777, 863
Bsp143I GATC 5 cut(s) 24, 262, 778, 885, 963
Bsp1720I GCTNAGC 1 cut(s) 242
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 5 cut(s) 15, 62, 166, 252, 416
BspANI GGCC 4 cut(s) 169, 251, 666, 792
BspCNI CTCAG 2 cut(s) 255, 837
BspFNI CGCG 2 cut(s) 254, 416
BspLI GGNNCC 2 cut(s) 269, 712
BspPI GGATC 1 cut(s) 893
BsrFI RCCGGY 2 cut(s) 48, 169
BsrI ACTGG 2 cut(s) 712, 928
BssAI RCCGGY 2 cut(s) 48, 169
BssECI CCNNGG 2 cut(s) 288, 667
BssMI GATC 5 cut(s) 24, 262, 778, 885, 963
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 667
Bst2UI CCWGG 3 cut(s) 290, 534, 747
Bst4CI ACNGT 4 cut(s) 203, 459, 545, 786
BstACI GRCGYC 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 902
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 2 cut(s) 242, 845
BstDSI CCRYGG 1 cut(s) 667
BstF5I GGATG 4 cut(s) 335, 355, 827, 951
BstFNI CGCG 2 cut(s) 254, 416
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 3 cut(s) 241, 346, 416
BstKTI GATC 5 cut(s) 27, 265, 781, 888, 966
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 5 cut(s) 24, 262, 778, 885, 963
BstMCI CGRYCG 3 cut(s) 27, 31, 170
BstNI CCWGG 3 cut(s) 290, 534, 747
BstSCI CCNGG 4 cut(s) 221, 288, 532, 745
BstSFI CTRYAG 1 cut(s) 891
BstUI CGCG 2 cut(s) 254, 416
BstX2I RGATCY 2 cut(s) 885, 963
BstYI RGATCY 2 cut(s) 885, 963
BstZI CGGCCG 1 cut(s) 167
BsuRI GGCC 4 cut(s) 169, 251, 666, 792
BtgI CCRYGG 1 cut(s) 667
BtsCI GGATG 4 cut(s) 335, 355, 827, 951
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 3 cut(s) 550, 592, 921
Cac8I GCNNGC 1 cut(s) 418
CaiI CAGNNNCTG 1 cut(s) 94
CfoI GCGC 3 cut(s) 241, 346, 416
Cfr10I RCCGGY 2 cut(s) 48, 169
CseI GACGC 1 cut(s) 409
Csp6I GTAC 4 cut(s) 409, 460, 467, 930
CspAI ACCGGT 1 cut(s) 48
CviAII CATG 3 cut(s) 341, 668, 897
CviQI GTAC 4 cut(s) 409, 460, 467, 930
DdeI CTNAG 2 cut(s) 242, 845
DpnI GATC 5 cut(s) 26, 264, 780, 887, 965
DpnII GATC 5 cut(s) 24, 262, 778, 885, 963
EaeI YGGCCR 3 cut(s) 167, 664, 790
EagI CGGCCG 1 cut(s) 167
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 2 cut(s) 299, 861
EclXI CGGCCG 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 667
Eco24I GRGCYC 3 cut(s) 37, 301, 863
Eco47III AGCGCT 1 cut(s) 240
Eco52I CGGCCG 1 cut(s) 167
Eco53kI GAGCTC 2 cut(s) 299, 861
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 36, 294
EcoICRI GAGCTC 2 cut(s) 299, 861
EcoRII CCWGG 3 cut(s) 288, 532, 745
EcoT14I CCWWGG 1 cut(s) 667
EcoT22I ATGCAT 2 cut(s) 907, 951
EcoT38I GRGCYC 3 cut(s) 37, 301, 863
ErhI CCWWGG 1 cut(s) 667
FaeI CATG 3 cut(s) 344, 671, 900
FaqI GGGAC 1 cut(s) 407
FatI CATG 3 cut(s) 340, 667, 896
FauI CCCGC 2 cut(s) 69, 409
FauNDI CATATG 1 cut(s) 951
FblI GTMKAC 1 cut(s) 498
FokI GGATG 4 cut(s) 342, 342, 814, 958
FriOI GRGCYC 3 cut(s) 37, 301, 863
FspBI CTAG 1 cut(s) 879
GlaI GCGC 3 cut(s) 240, 345, 415
GsuI CTGGAG 1 cut(s) 729
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 4 cut(s) 169, 251, 666, 792
HapII CCGG 4 cut(s) 49, 170, 222, 347
HgaI GACGC 1 cut(s) 409
HhaI GCGC 3 cut(s) 241, 346, 416
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 3 cut(s) 344, 671, 900
Hin6I GCGC 3 cut(s) 239, 344, 414
HinP1I GCGC 3 cut(s) 239, 344, 414
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 9 cut(s) 70, 191, 337, 386, 446, 554, 637, 681, 814
HpaII CCGG 4 cut(s) 49, 170, 222, 347
HphI GGTGA 2 cut(s) 548, 864
Hpy166II GTNNAC 3 cut(s) 279, 499, 797
Hpy188I TCNGA 3 cut(s) 162, 196, 865
Hpy188III TCNNGA 3 cut(s) 74, 233, 839
Hpy8I GTNNAC 3 cut(s) 279, 499, 797
Hpy99I CGWCG 2 cut(s) 200, 214
HpyAV CCTTC 2 cut(s) 885, 936
HpyCH4III ACNGT 4 cut(s) 203, 459, 545, 786
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 5 cut(s) 551, 738, 827, 905, 949
HpyF3I CTNAG 2 cut(s) 242, 845
HpySE526I ACGT 1 cut(s) 209
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 3 cut(s) 344, 671, 900
HspAI GCGC 3 cut(s) 239, 344, 414
Kzo9I GATC 5 cut(s) 24, 262, 778, 885, 963
LmnI GCTCC 3 cut(s) 596, 772, 858
LweI GCATC 5 cut(s) 166, 538, 619, 836, 936
MaeI CTAG 1 cut(s) 879
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 3 cut(s) 8, 351, 443
MalI GATC 5 cut(s) 26, 264, 780, 887, 965
MboI GATC 5 cut(s) 24, 262, 778, 885, 963
MflI RGATCY 2 cut(s) 885, 963
MhlI GDGCHC 4 cut(s) 37, 301, 777, 863
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 2 cut(s) 256, 833
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 6 cut(s) 200, 331, 440, 563, 646, 690
MmeI TCCRAC 3 cut(s) 219, 653, 863
MnlI CCTC 9 cut(s) 156, 241, 327, 435, 522, 599, 724, 753, 840
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 2 cut(s) 907, 951
MroXI GAANNNNTTC 1 cut(s) 50
MscI TGGCCA 1 cut(s) 666
MseI TTAA 2 cut(s) 434, 704
Msp20I TGGCCA 1 cut(s) 666
MspI CCGG 4 cut(s) 49, 170, 222, 347
MspR9I CCNGG 4 cut(s) 223, 290, 534, 747
MvaI CCWGG 3 cut(s) 290, 534, 747
MvnI CGCG 2 cut(s) 254, 416
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 1 cut(s) 951
NdeII GATC 5 cut(s) 24, 262, 778, 885, 963
NlaIII CATG 3 cut(s) 344, 671, 900
NlaIV GGNNCC 2 cut(s) 269, 712
NmuCI GTSAC 2 cut(s) 8, 443
NsiI ATGCAT 2 cut(s) 907, 951
PaeR7I CTCGAG 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 3 cut(s) 70, 386, 814
Pfl23II CGTACG 1 cut(s) 408
PflFI GACNNNGTC 1 cut(s) 449
PinAI ACCGGT 1 cut(s) 48
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 6 cut(s) 199, 331, 440, 562, 645, 689
PpsI GAGTC 6 cut(s) 199, 331, 440, 562, 645, 689
Psp124BI GAGCTC 2 cut(s) 301, 863
Psp6I CCWGG 3 cut(s) 288, 532, 745
PspGI CCWGG 3 cut(s) 288, 532, 745
PspLI CGTACG 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 269, 712
PspXI VCTCGAGB 1 cut(s) 294
PsrI GAACNNNNNNTAC 2 cut(s) 38, 70
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 2 cut(s) 885, 963
PsyI GACNNNGTC 1 cut(s) 449
PvuI CGATCG 1 cut(s) 27
RsaI GTAC 4 cut(s) 410, 461, 468, 931
RsaNI GTAC 4 cut(s) 409, 460, 467, 930
SacI GAGCTC 2 cut(s) 301, 863
SaqAI TTAA 2 cut(s) 434, 704
Sau3AI GATC 5 cut(s) 24, 262, 778, 885, 963
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 6 cut(s) 200, 331, 440, 563, 646, 690
ScrFI CCNGG 4 cut(s) 223, 290, 534, 747
SduI GDGCHC 4 cut(s) 37, 301, 777, 863
SfaNI GCATC 5 cut(s) 166, 538, 619, 836, 936
SfcI CTRYAG 1 cut(s) 891
Sfr274I CTCGAG 1 cut(s) 294
SlaI CTCGAG 1 cut(s) 294
SmlI CTYRAG 3 cut(s) 272, 294, 715
SmoI CTYRAG 3 cut(s) 272, 294, 715
Sse9I AATT 2 cut(s) 256, 833
SsiI CCGC 5 cut(s) 15, 62, 166, 252, 416
SspI AATATT 2 cut(s) 649, 767
SspMI CTAG 1 cut(s) 879
SstI GAGCTC 2 cut(s) 301, 863
StyD4I CCNGG 4 cut(s) 221, 288, 532, 745
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 4 cut(s) 203, 459, 545, 786
TaiI ACGT 1 cut(s) 212
TaqI TCGA 3 cut(s) 23, 73, 295
TaqII GACCGA 1 cut(s) 17
TasI AATT 2 cut(s) 256, 833
TatI WGTACW 2 cut(s) 459, 929
TauI GCSGC 2 cut(s) 169, 254
TfiI GAWTC 3 cut(s) 70, 386, 814
Tru1I TTAA 2 cut(s) 434, 704
Tru9I TTAA 2 cut(s) 434, 704
TscAI CASTG 3 cut(s) 550, 592, 928
TseFI GTSAC 2 cut(s) 8, 443
Tsp45I GTSAC 2 cut(s) 8, 443
TspDTI ATGAA 2 cut(s) 626, 738
TspRI CASTG 3 cut(s) 550, 592, 928
Tth111I GACNNNGTC 1 cut(s) 449
XapI RAATTY 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 498
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 1 cut(s) 879
ZrmI AGTACT 1 cut(s) 461
Zsp2I ATGCAT 2 cut(s) 907, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.