MD13G1160400.v1.1

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009360
Physical Location & Seq
Reverse (-)
12595860 .. 12597013
1154 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1160400.v1.1.491

Sequence Viewer

Length: 447 bp
ATGTCAGGCGTTTCGATTGCTCGGATTCGGGGAGATAATCGCTTCTACAACCCGCCAGCTATACGGCAGCAGCAGCAGAGAAAAAAGCTAGAGGAGGAGAAGCATCAGAGGCAGTCGGTGGATTCAGATGACTGTGAGACTTCTTACAGTTCCGGTTCGGGTGATGCTACGAATTTGGATCGGTTTTTGGAGTACACCACACCTGTGGTTGCGGCTCAGTATTTTTCCAAGACAAGTGTGAGGGGATGGAGGAGTCGCGAGGCAGAATTACATCCATACTTTGTGCTTGGAGATTTATGGGAATCTTTCAAGGAGTGGAGCGCGTACGGTGCAGGTGTCCCTCTTTTGTTGAATGGGAGTGACTCTGTTATCCAGTACTATGTTCCTTACTTGTCCGCTATTCAGCTCTATGTAGACCCATCAAAGTCCTCAACGAGAATAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

149

Amino Acids

16.91

Weight (kDa)

6.73

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 57 - 144 4.5e-31 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 414
AccII CGCG 2 cut(s) 258, 323
AciI CCGC 3 cut(s) 53, 212, 396
AclWI GGATC 1 cut(s) 186
AcsI RAATTY 1 cut(s) 172
AfaI GTAC 3 cut(s) 194, 326, 377
AgsI TTSAA 2 cut(s) 310, 352
AleI CACNNNNGTG 1 cut(s) 203
AluBI AGCT 3 cut(s) 59, 88, 406
AluI AGCT 3 cut(s) 59, 88, 406
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 186
ApeKI GCWGC 3 cut(s) 67, 70, 73
ApoI RAATTY 1 cut(s) 172
AspLEI GCGC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 173
BbvI GCAGC 3 cut(s) 79, 82, 85
BccI CCATC 2 cut(s) 240, 427
BceAI ACGGC 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 323
BisI GCNGC 4 cut(s) 68, 71, 74, 213
BlsI GCNGC 4 cut(s) 69, 72, 75, 214
BmcAI AGTACT 1 cut(s) 377
BmsI GCATC 2 cut(s) 112, 154
BsaWI WCCGGW 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 373
BseGI GGATG 2 cut(s) 251, 271
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 3 cut(s) 107, 110, 265
BseXI GCAGC 3 cut(s) 79, 82, 85
BsgI GTGCAG 1 cut(s) 351
Bsh1236I CGCG 2 cut(s) 258, 323
BsiSI CCGG 1 cut(s) 153
BsiWI CGTACG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 323
Bsp143I GATC 1 cut(s) 178
Bsp68I TCGCGA 1 cut(s) 258
BspACI CCGC 3 cut(s) 53, 212, 396
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 2 cut(s) 258, 323
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 1 cut(s) 186
BsrI ACTGG 1 cut(s) 373
BssMI GATC 1 cut(s) 178
Bst4CI ACNGT 3 cut(s) 134, 149, 329
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 251, 271
BstFNI CGCG 2 cut(s) 258, 323
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 178
BstMWI GCNNNNNNNGC 3 cut(s) 73, 109, 329
BstUI CGCG 2 cut(s) 258, 323
BstV1I GCAGC 3 cut(s) 79, 82, 85
BstXI CCANNNNNNTGG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 251, 271
BtuMI TCGCGA 1 cut(s) 258
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 1 cut(s) 57
CfoI GCGC 1 cut(s) 323
Csp6I GTAC 3 cut(s) 193, 325, 376
CviJI RGCY 4 cut(s) 59, 88, 215, 406
CviKI_1 RGCY 4 cut(s) 59, 88, 215, 406
CviQI GTAC 3 cut(s) 193, 325, 376
DdeI CTNAG 1 cut(s) 216
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
FaiI YATR 5 cut(s) 62, 277, 298, 381, 411
FaqI GGGAC 1 cut(s) 323
FauI CCCGC 1 cut(s) 60
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 4 cut(s) 68, 71, 74, 213
FokI GGATG 2 cut(s) 258, 258
Fsp4HI GCNGC 4 cut(s) 68, 71, 74, 213
FspBI CTAG 1 cut(s) 89
GlaI GCGC 1 cut(s) 322
GluI GCNGC 4 cut(s) 68, 71, 74, 213
HapII CCGG 1 cut(s) 153
HhaI GCGC 1 cut(s) 323
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HinfI GANTC 5 cut(s) 25, 122, 253, 302, 362
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 2 cut(s) 195, 415
Hpy188I TCNGA 3 cut(s) 24, 108, 127
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 2 cut(s) 195, 415
HpyCH4III ACNGT 3 cut(s) 134, 149, 329
HpyCH4V TGCA 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 109, 329
HpyF3I CTNAG 1 cut(s) 216
HspAI GCGC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 178
LmnI GCTCC 1 cut(s) 318
LpnPI CCDG 5 cut(s) 69, 166, 216, 318, 386
Lsp1109I GCAGC 3 cut(s) 79, 82, 85
LweI GCATC 2 cut(s) 112, 154
MaeI CTAG 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 359
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MluCI AATT 2 cut(s) 172, 266
MlyI GAGTC 2 cut(s) 262, 356
MnlI CCTC 8 cut(s) 85, 88, 102, 234, 243, 253, 351, 439
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 1 cut(s) 153
MvnI CGCG 2 cut(s) 258, 323
MwoI GCNNNNNNNGC 3 cut(s) 73, 109, 329
NdeII GATC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 359
NruI TCGCGA 1 cut(s) 258
OliI CACNNNNGTG 1 cut(s) 203
PaqCI CACCTGC 1 cut(s) 323
PfeI GAWTC 3 cut(s) 25, 122, 302
Pfl23II CGTACG 1 cut(s) 324
PkrI GCNGC 4 cut(s) 69, 72, 75, 214
PleI GAGTC 2 cut(s) 261, 356
PpsI GAGTC 2 cut(s) 261, 356
PspLI CGTACG 1 cut(s) 324
RruI TCGCGA 1 cut(s) 258
RsaI GTAC 3 cut(s) 194, 326, 377
RsaNI GTAC 3 cut(s) 193, 325, 376
RseI CAYNNNNRTG 1 cut(s) 203
SatI GCNGC 4 cut(s) 68, 71, 74, 213
Sau3AI GATC 1 cut(s) 178
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 2 cut(s) 262, 356
SetI ASST 6 cut(s) 61, 90, 205, 337, 408, 446
SfaNI GCATC 2 cut(s) 112, 154
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 2 cut(s) 172, 266
SsiI CCGC 3 cut(s) 53, 212, 396
SspMI CTAG 1 cut(s) 89
TaaI ACNGT 3 cut(s) 134, 149, 329
TaqI TCGA 1 cut(s) 14
TasI AATT 2 cut(s) 172, 266
TatI WGTACW 2 cut(s) 192, 375
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 3 cut(s) 25, 122, 302
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 3 cut(s) 67, 70, 73
Tsp45I GTSAC 1 cut(s) 359
XapI RAATTY 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 1 cut(s) 89
ZrmI AGTACT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.