Rh4AG251300

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009360
Physical Location & Seq
Forward (+)
56459607 .. 56463974
4368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG251300.1

Sequence Viewer

Length: 948 bp
ATGTCTGGTCACGGCGGAGTTTCGATCGGTCGGGCTCGGGGCGAGAACCGGTTCTACAACCCGCCCCCGATTCGAGAGCGACAACACCAGCAACAGCTTCAGCGGCAGCAGAAGCTTGAACAGGAGAAGCAGCATCATCAGAGGCGGCCGGTGTCGGTGTCGGTGGTGGAGTCCGACGACTGTGCTACGTCGCATAGTTCCGGGTCGGGTCGGGAAGCGCTCAGCGAGGCCGCGAATTTAGATCGGTTCCTTGAGTTCACAACGCCCCTGGCTCGAGCTCATTTTTTGCCCAAGACGAGTAGCAGGGGATGGAGGACTCATGAGCCGGAGTTACATCCCTATTTTGTGCTTGGAGATTTATGGGAATCTTTCAAGGAGTGGAGTGCGTACGGCGCGGGTGTCCCTCTTTTGTTAAATGGGAGTGACTCTGTCATACAGTACTATGTACCTTACTTGTCTGCCATTCAGCTCTATGTAGACCCATCAAAGCCTCCCACAAGGATAAGGAAGCCTGGTGATGACAGTGATGCAGAGTCATCGAGGGAGACAAGTAGTGATGGCAGCAGTGATTATGGAGCAGAAAGAGGAATCAATGGTGTTGTTGATGATGCTTGGAGTCGGCAGAATATTGCTGATGTAAATGGCCATGGTTGGAATAGAGTCTTACCAAGAGATAAACCCTTAACTGGTTCCTCAAGTGATGAAAGTGAGATTTGCAATCCTCCAGGTCAGCTTGTATTTCAATATTTGGAGCACGATCAACCGTTTGGCCGTGAACCTTTGGCTGATAAGATTCAAGTTCTTGCATCCCAATTTCCAGAGCTGAGGACTTACAGGAGCTCTGACTTATCACCTTCTAGTTGGATCTCTGTAGCATGGTATGCATTTATTTTTGTATTCACTGGTAGTACAAATAAAGGAAGGATGCATATGAGATTACAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

315

Amino Acids

35.46

Weight (kDa)

6.13

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 78 - 296 9.6e-63 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 477
AccII CGCG 2 cut(s) 233, 395
AciI CCGC 6 cut(s) 15, 62, 103, 145, 231, 395
AclWI GGATC 1 cut(s) 872
AcoI YGGCCR 3 cut(s) 146, 643, 769
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 4 cut(s) 389, 440, 447, 910
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 686
AgeI ACCGGT 1 cut(s) 48
AgsI TTSAA 4 cut(s) 119, 373, 743, 797
AjnI CCWGG 3 cut(s) 267, 511, 724
AluBI AGCT 7 cut(s) 97, 115, 278, 469, 733, 823, 840
AluI AGCT 7 cut(s) 97, 115, 278, 469, 733, 823, 840
Alw21I GWGCWC 3 cut(s) 280, 756, 842
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 1 cut(s) 872
Ama87I CYCGRG 2 cut(s) 36, 273
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 4 cut(s) 146, 228, 643, 769
ApeKI GCWGC 3 cut(s) 106, 130, 561
ApoI RAATTY 1 cut(s) 235
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 2 cut(s) 220, 395
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 527, 843
AvaI CYCGRG 2 cut(s) 36, 273
BalI TGGCCA 1 cut(s) 645
BanII GRGCYC 3 cut(s) 37, 280, 842
Bbv12I GWGCWC 3 cut(s) 280, 756, 842
BbvCI CCTCAGC 1 cut(s) 824
BbvI GCAGC 3 cut(s) 118, 142, 573
BccI CCATC 3 cut(s) 303, 490, 551
BceAI ACGGC 3 cut(s) 28, 406, 756
BcgI CGANNNNNNTGC 4 cut(s) 164, 198, 519, 553
BciT130I CCWGG 3 cut(s) 269, 513, 726
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 539
BfaI CTAG 1 cut(s) 858
BfmI CTRYAG 1 cut(s) 870
BfoI RGCGCY 1 cut(s) 221
BglII AGATCT 1 cut(s) 942
BisI GCNGC 6 cut(s) 104, 107, 131, 146, 231, 562
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 6 cut(s) 105, 108, 132, 147, 232, 563
BmcAI AGTACT 1 cut(s) 440
Bme1390I CCNGG 4 cut(s) 202, 269, 513, 726
BmeT110I CYCGRG 2 cut(s) 36, 273
BmiI GGNNCC 2 cut(s) 248, 691
BmrFI CCNGG 4 cut(s) 202, 269, 513, 726
BmsI GCATC 5 cut(s) 142, 517, 598, 815, 915
BpmI CTGGAG 1 cut(s) 708
Bpu10I CCTNAGC 1 cut(s) 824
Bpu1102I GCTNAGC 1 cut(s) 221
BpuEI CTTGAG 2 cut(s) 272, 679
BpuMI CCSGG 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 267, 646
BsaWI WCCGGW 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse118I RCCGGY 2 cut(s) 48, 148
Bse1I ACTGG 2 cut(s) 691, 907
BseBI CCWGG 3 cut(s) 269, 513, 726
BseDI CCNNGG 2 cut(s) 267, 646
BseGI GGATG 4 cut(s) 314, 334, 806, 930
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMII CTCAG 2 cut(s) 235, 815
BseNI ACTGG 2 cut(s) 691, 907
BseX3I CGGCCG 1 cut(s) 146
BseXI GCAGC 3 cut(s) 118, 142, 573
Bsh1236I CGCG 2 cut(s) 233, 395
Bsh1285I CGRYCG 3 cut(s) 27, 31, 149
BshFI GGCC 4 cut(s) 148, 230, 645, 771
BshTI ACCGGT 1 cut(s) 48
BsiEI CGRYCG 3 cut(s) 27, 31, 149
BsiHKAI GWGCWC 3 cut(s) 280, 756, 842
BsiHKCI CYCGRG 2 cut(s) 36, 273
BsiSI CCGG 4 cut(s) 49, 149, 201, 326
BsiWI CGTACG 1 cut(s) 387
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 539
BsmFI GGGAC 1 cut(s) 386
BsnI GGCC 4 cut(s) 148, 230, 645, 771
BsoBI CYCGRG 2 cut(s) 36, 273
Bsp1286I GDGCHC 4 cut(s) 37, 280, 756, 842
Bsp143I GATC 5 cut(s) 24, 241, 757, 864, 942
Bsp1720I GCTNAGC 1 cut(s) 221
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 6 cut(s) 15, 62, 103, 145, 231, 395
BspANI GGCC 4 cut(s) 148, 230, 645, 771
BspCNI CTCAG 2 cut(s) 234, 816
BspFNI CGCG 2 cut(s) 233, 395
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 248, 691
BspPI GGATC 1 cut(s) 872
BsrFI RCCGGY 2 cut(s) 48, 148
BsrI ACTGG 2 cut(s) 691, 907
BssAI RCCGGY 2 cut(s) 48, 148
BssECI CCNNGG 2 cut(s) 267, 646
BssMI GATC 5 cut(s) 24, 241, 757, 864, 942
BssT1I CCWWGG 1 cut(s) 646
Bst2UI CCWGG 3 cut(s) 269, 513, 726
Bst4CI ACNGT 4 cut(s) 182, 438, 524, 765
BstAPI GCANNNNNTGC 1 cut(s) 881
BstDEI CTNAG 2 cut(s) 221, 824
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 4 cut(s) 314, 334, 806, 930
BstFNI CGCG 2 cut(s) 233, 395
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 395
BstKTI GATC 5 cut(s) 27, 244, 760, 867, 945
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 5 cut(s) 24, 241, 757, 864, 942
BstMCI CGRYCG 3 cut(s) 27, 31, 149
BstMWI GCNNNNNNNGC 4 cut(s) 103, 112, 392, 881
BstNI CCWGG 3 cut(s) 269, 513, 726
BstSCI CCNGG 4 cut(s) 200, 267, 511, 724
BstSFI CTRYAG 1 cut(s) 870
BstUI CGCG 2 cut(s) 233, 395
BstV1I GCAGC 3 cut(s) 118, 142, 573
BstX2I RGATCY 2 cut(s) 864, 942
BstYI RGATCY 2 cut(s) 864, 942
BstZI CGGCCG 1 cut(s) 146
BsuRI GGCC 4 cut(s) 148, 230, 645, 771
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 4 cut(s) 314, 334, 806, 930
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 3 cut(s) 529, 571, 900
CciI TCATGA 1 cut(s) 319
CfoI GCGC 2 cut(s) 220, 395
Cfr10I RCCGGY 2 cut(s) 48, 148
Csp6I GTAC 4 cut(s) 388, 439, 446, 909
CspAI ACCGGT 1 cut(s) 48
CviAII CATG 3 cut(s) 320, 647, 876
CviQI GTAC 4 cut(s) 388, 439, 446, 909
DdeI CTNAG 2 cut(s) 221, 824
DpnI GATC 5 cut(s) 26, 243, 759, 866, 944
DpnII GATC 5 cut(s) 24, 241, 757, 864, 942
EaeI YGGCCR 3 cut(s) 146, 643, 769
EagI CGGCCG 1 cut(s) 146
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 2 cut(s) 278, 840
EclXI CGGCCG 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 646
Eco24I GRGCYC 3 cut(s) 37, 280, 842
Eco47III AGCGCT 1 cut(s) 219
Eco52I CGGCCG 1 cut(s) 146
Eco53kI GAGCTC 2 cut(s) 278, 840
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 36, 273
EcoICRI GAGCTC 2 cut(s) 278, 840
EcoRII CCWGG 3 cut(s) 267, 511, 724
EcoT14I CCWWGG 1 cut(s) 646
EcoT22I ATGCAT 2 cut(s) 886, 930
EcoT38I GRGCYC 3 cut(s) 37, 280, 842
ErhI CCWWGG 1 cut(s) 646
FaeI CATG 3 cut(s) 323, 650, 879
FaqI GGGAC 1 cut(s) 386
FatI CATG 3 cut(s) 319, 646, 875
FauI CCCGC 2 cut(s) 69, 388
FauNDI CATATG 1 cut(s) 930
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 6 cut(s) 104, 107, 131, 146, 231, 562
FokI GGATG 4 cut(s) 321, 321, 793, 937
FriOI GRGCYC 3 cut(s) 37, 280, 842
Fsp4HI GCNGC 6 cut(s) 104, 107, 131, 146, 231, 562
FspBI CTAG 1 cut(s) 858
GlaI GCGC 2 cut(s) 219, 394
GluI GCNGC 6 cut(s) 104, 107, 131, 146, 231, 562
GsuI CTGGAG 1 cut(s) 708
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 4 cut(s) 148, 230, 645, 771
HapII CCGG 4 cut(s) 49, 149, 201, 326
HhaI GCGC 2 cut(s) 220, 395
Hin1II CATG 3 cut(s) 323, 650, 879
Hin6I GCGC 2 cut(s) 218, 393
HinP1I GCGC 2 cut(s) 218, 393
HindIII AAGCTT 1 cut(s) 113
HpaII CCGG 4 cut(s) 49, 149, 201, 326
HphI GGTGA 2 cut(s) 527, 843
Hpy166II GTNNAC 3 cut(s) 258, 478, 776
Hpy188I TCNGA 3 cut(s) 141, 175, 844
Hpy188III TCNNGA 4 cut(s) 74, 212, 320, 818
Hpy8I GTNNAC 3 cut(s) 258, 478, 776
Hpy99I CGWCG 2 cut(s) 179, 193
HpyAV CCTTC 2 cut(s) 864, 915
HpyCH4III ACNGT 4 cut(s) 182, 438, 524, 765
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 5 cut(s) 530, 717, 806, 884, 928
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 112, 392, 881
HpyF3I CTNAG 2 cut(s) 221, 824
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 3 cut(s) 323, 650, 879
HspAI GCGC 2 cut(s) 218, 393
Kzo9I GATC 5 cut(s) 24, 241, 757, 864, 942
LmnI GCTCC 3 cut(s) 575, 751, 837
Lsp1109I GCAGC 3 cut(s) 118, 142, 573
LweI GCATC 5 cut(s) 142, 517, 598, 815, 915
MaeI CTAG 1 cut(s) 858
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 3 cut(s) 8, 330, 422
MalI GATC 5 cut(s) 26, 243, 759, 866, 944
MboI GATC 5 cut(s) 24, 241, 757, 864, 942
MflI RGATCY 2 cut(s) 864, 942
MhlI GDGCHC 4 cut(s) 37, 280, 756, 842
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 2 cut(s) 235, 812
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 6 cut(s) 179, 310, 419, 542, 625, 669
MmeI TCCRAC 3 cut(s) 198, 632, 842
Mox20I TGGCCA 1 cut(s) 645
Mph1103I ATGCAT 2 cut(s) 886, 930
MroXI GAANNNNTTC 1 cut(s) 50
MscI TGGCCA 1 cut(s) 645
MseI TTAA 2 cut(s) 413, 683
Msp20I TGGCCA 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 4 cut(s) 49, 149, 201, 326
MspR9I CCNGG 4 cut(s) 202, 269, 513, 726
MvaI CCWGG 3 cut(s) 269, 513, 726
MvnI CGCG 2 cut(s) 233, 395
MwoI GCNNNNNNNGC 4 cut(s) 103, 112, 392, 881
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 646
NdeI CATATG 1 cut(s) 930
NdeII GATC 5 cut(s) 24, 241, 757, 864, 942
NlaIII CATG 3 cut(s) 323, 650, 879
NlaIV GGNNCC 2 cut(s) 248, 691
NmuCI GTSAC 2 cut(s) 8, 422
NsiI ATGCAT 2 cut(s) 886, 930
PaeR7I CTCGAG 1 cut(s) 273
PagI TCATGA 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 4 cut(s) 70, 365, 588, 793
Pfl23II CGTACG 1 cut(s) 387
PflFI GACNNNGTC 1 cut(s) 428
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 6 cut(s) 105, 108, 132, 147, 232, 563
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 6 cut(s) 178, 310, 419, 541, 624, 668
PpsI GAGTC 6 cut(s) 178, 310, 419, 541, 624, 668
Psp124BI GAGCTC 2 cut(s) 280, 842
Psp6I CCWGG 3 cut(s) 267, 511, 724
PspGI CCWGG 3 cut(s) 267, 511, 724
PspLI CGTACG 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 248, 691
PspXI VCTCGAGB 1 cut(s) 273
PsrI GAACNNNNNNTAC 2 cut(s) 38, 70
PsuI RGATCY 2 cut(s) 864, 942
PsyI GACNNNGTC 1 cut(s) 428
PvuI CGATCG 1 cut(s) 27
RsaI GTAC 4 cut(s) 389, 440, 447, 910
RsaNI GTAC 4 cut(s) 388, 439, 446, 909
SacI GAGCTC 2 cut(s) 280, 842
SaqAI TTAA 2 cut(s) 413, 683
SatI GCNGC 6 cut(s) 104, 107, 131, 146, 231, 562
Sau3AI GATC 5 cut(s) 24, 241, 757, 864, 942
ScaI AGTACT 1 cut(s) 440
SchI GAGTC 6 cut(s) 179, 310, 419, 542, 625, 669
ScrFI CCNGG 4 cut(s) 202, 269, 513, 726
SduI GDGCHC 4 cut(s) 37, 280, 756, 842
SfaNI GCATC 5 cut(s) 142, 517, 598, 815, 915
SfcI CTRYAG 1 cut(s) 870
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmlI CTYRAG 3 cut(s) 251, 273, 694
SmoI CTYRAG 3 cut(s) 251, 273, 694
Sse9I AATT 2 cut(s) 235, 812
SsiI CCGC 6 cut(s) 15, 62, 103, 145, 231, 395
SspI AATATT 2 cut(s) 628, 746
SspMI CTAG 1 cut(s) 858
SstI GAGCTC 2 cut(s) 280, 842
StyD4I CCNGG 4 cut(s) 200, 267, 511, 724
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 4 cut(s) 182, 438, 524, 765
TaiI ACGT 1 cut(s) 191
TaqI TCGA 4 cut(s) 23, 73, 274, 539
TaqII GACCGA 1 cut(s) 17
TasI AATT 2 cut(s) 235, 812
TatI WGTACW 2 cut(s) 438, 908
TauI GCSGC 3 cut(s) 106, 148, 233
TfiI GAWTC 4 cut(s) 70, 365, 588, 793
Tru1I TTAA 2 cut(s) 413, 683
Tru9I TTAA 2 cut(s) 413, 683
TscAI CASTG 3 cut(s) 529, 571, 907
TseFI GTSAC 2 cut(s) 8, 422
TseI GCWGC 3 cut(s) 106, 130, 561
Tsp45I GTSAC 2 cut(s) 8, 422
TspDTI ATGAA 1 cut(s) 717
TspRI CASTG 3 cut(s) 529, 571, 907
Tth111I GACNNNGTC 1 cut(s) 428
XapI RAATTY 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 273
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 1 cut(s) 858
ZrmI AGTACT 1 cut(s) 440
Zsp2I ATGCAT 2 cut(s) 886, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.