Rh4CG267300

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009360
Physical Location & Seq
Forward (+)
52139072 .. 52142456
3385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG267300.1

Sequence Viewer

Length: 951 bp
ATGTCTGGTCACGGCGGAGTTTCGATCGGTCGGGCTCGGGGCGAGAACCGATTCTACAACCCGCCCCCGATTCGAGAGCGACAACACCAGCAACAGCTTCAGCAGCAGCAGCAGCAGCAGAAGCTTGAACAGGAGAAGCAGCAGCATCAGAGGCGGCCGGTGTCGGTGGTGGAGTCCGACGACTGTGCTACGTCGCATAGTTCCGGGTCGGGTCGGGAAGCGCTCAGCGAGGCCGCGAATTTAGATCGGTTCCTTGAGTTCACAACGCCCCTGGCTCGAGCTCATTTTTTGCCCAAGACGAGTAGCAGGGGATGGAGGACTCATGAGCCGGAGTTACATCCCTACTTTGTGCTTGGAGATTTGTGGGAATCTTTCAAGGAGTGGAGTGCGTACGGCGCGGGTGTCCCTCTTTTGTTAAATGGGAGTGACTCTGTCATACAGTACTATGTACCTTACTTGTCTGCCATTCAGCTCTATGTAGACCCATCAAAGCCTCCCACAAGGATAAGGAAGCCTGGTGATGACAGTGATGCAGAGTCATCGAGGGAGACAAGTAGTGATGGCAGCAGTGATTATGGAGCAGAAAGAGGAATCAATGGTGTTGTTGATGATGCTTGGAGTCGGCAGAATATTGCTGATGTAAATGGCCATGGTTGGAATAGAGTCTTACCAAGAGATAAACCCTTAACTGGTTCCTCAAGTGATGAAAGTGAGATTTGCAATCCTCCAGGTCAGCTTATATTTCAATATTTGGAGCACGATCAACCGTTTGGCCGTGAACCTTTGGCTGATAAGATTCAAGTTCTTGCATCCCAATTTCCAGAGCTGAGGACTTACAGGAGCTCGGACTTATCACCTTCTAGTTGGATCTCTGTAGCATGGTATGCATTTATTTTTGTATTCACTGGTAGTACAAATAAGGGAAGGATGCATGTGAGATTACAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

316

Amino Acids

35.6

Weight (kDa)

5.94

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 79 - 297 8.4e-63 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 480
AccII CGCG 2 cut(s) 236, 398
AciI CCGC 5 cut(s) 15, 62, 154, 234, 398
AclWI GGATC 1 cut(s) 875
AcoI YGGCCR 3 cut(s) 155, 646, 772
AcsI RAATTY 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 4 cut(s) 392, 443, 450, 913
AfeI AGCGCT 1 cut(s) 222
AfiI CCNNNNNNNGG 1 cut(s) 689
AgsI TTSAA 4 cut(s) 128, 376, 746, 800
AjnI CCWGG 3 cut(s) 270, 514, 727
AluBI AGCT 7 cut(s) 97, 124, 281, 472, 736, 826, 843
AluI AGCT 7 cut(s) 97, 124, 281, 472, 736, 826, 843
Alw21I GWGCWC 3 cut(s) 283, 759, 845
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 1 cut(s) 875
Ama87I CYCGRG 2 cut(s) 36, 276
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 4 cut(s) 155, 231, 646, 772
ApeKI GCWGC 8 cut(s) 103, 106, 109, 112, 115, 139, 142, 564
ApoI RAATTY 1 cut(s) 238
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 2 cut(s) 223, 398
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 2 cut(s) 530, 846
AvaI CYCGRG 2 cut(s) 36, 276
BalI TGGCCA 1 cut(s) 648
BanII GRGCYC 3 cut(s) 37, 283, 845
Bbv12I GWGCWC 3 cut(s) 283, 759, 845
BbvCI CCTCAGC 1 cut(s) 827
BbvI GCAGC 8 cut(s) 115, 118, 121, 124, 127, 151, 154, 576
BccI CCATC 3 cut(s) 306, 493, 554
BceAI ACGGC 3 cut(s) 28, 409, 759
BcgI CGANNNNNNTGC 4 cut(s) 167, 201, 522, 556
BciT130I CCWGG 3 cut(s) 272, 516, 729
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 1 cut(s) 861
BfmI CTRYAG 1 cut(s) 873
BfoI RGCGCY 1 cut(s) 224
BglII AGATCT 1 cut(s) 945
BlpI GCTNAGC 1 cut(s) 224
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 4 cut(s) 205, 272, 516, 729
BmeT110I CYCGRG 2 cut(s) 36, 276
BmiI GGNNCC 2 cut(s) 251, 694
BmrFI CCNGG 4 cut(s) 205, 272, 516, 729
BmsI GCATC 5 cut(s) 154, 520, 601, 818, 918
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 827
Bpu1102I GCTNAGC 1 cut(s) 224
BpuEI CTTGAG 2 cut(s) 275, 682
BpuMI CCSGG 1 cut(s) 205
BsaJI CCNNGG 2 cut(s) 270, 649
Bsc4I CCNNNNNNNGG 1 cut(s) 689
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 2 cut(s) 694, 910
BseBI CCWGG 3 cut(s) 272, 516, 729
BseDI CCNNGG 2 cut(s) 270, 649
BseGI GGATG 4 cut(s) 317, 337, 809, 933
BseLI CCNNNNNNNGG 1 cut(s) 689
BseMII CTCAG 2 cut(s) 238, 818
BseNI ACTGG 2 cut(s) 694, 910
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 8 cut(s) 115, 118, 121, 124, 127, 151, 154, 576
Bsh1236I CGCG 2 cut(s) 236, 398
Bsh1285I CGRYCG 3 cut(s) 27, 31, 158
BshFI GGCC 4 cut(s) 157, 233, 648, 774
BsiEI CGRYCG 3 cut(s) 27, 31, 158
BsiHKAI GWGCWC 3 cut(s) 283, 759, 845
BsiHKCI CYCGRG 2 cut(s) 36, 276
BsiSI CCGG 3 cut(s) 158, 204, 329
BsiWI CGTACG 1 cut(s) 390
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 689
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 4 cut(s) 157, 233, 648, 774
BsoBI CYCGRG 2 cut(s) 36, 276
Bsp1286I GDGCHC 4 cut(s) 37, 283, 759, 845
Bsp143I GATC 5 cut(s) 24, 244, 760, 867, 945
Bsp1720I GCTNAGC 1 cut(s) 224
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 5 cut(s) 15, 62, 154, 234, 398
BspANI GGCC 4 cut(s) 157, 233, 648, 774
BspCNI CTCAG 2 cut(s) 237, 819
BspFNI CGCG 2 cut(s) 236, 398
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 251, 694
BspPI GGATC 1 cut(s) 875
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 2 cut(s) 694, 910
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 270, 649
BssMI GATC 5 cut(s) 24, 244, 760, 867, 945
BssT1I CCWWGG 1 cut(s) 649
Bst2UI CCWGG 3 cut(s) 272, 516, 729
Bst4CI ACNGT 4 cut(s) 185, 441, 527, 768
BstAPI GCANNNNNTGC 1 cut(s) 884
BstDEI CTNAG 2 cut(s) 224, 827
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 4 cut(s) 317, 337, 809, 933
BstFNI CGCG 2 cut(s) 236, 398
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 2 cut(s) 223, 398
BstKTI GATC 5 cut(s) 27, 247, 763, 870, 948
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 5 cut(s) 24, 244, 760, 867, 945
BstMCI CGRYCG 3 cut(s) 27, 31, 158
BstMWI GCNNNNNNNGC 8 cut(s) 103, 109, 112, 115, 121, 151, 395, 884
BstNI CCWGG 3 cut(s) 272, 516, 729
BstNSI RCATGY 1 cut(s) 935
BstSCI CCNGG 4 cut(s) 203, 270, 514, 727
BstSFI CTRYAG 1 cut(s) 873
BstUI CGCG 2 cut(s) 236, 398
BstV1I GCAGC 8 cut(s) 115, 118, 121, 124, 127, 151, 154, 576
BstX2I RGATCY 2 cut(s) 867, 945
BstYI RGATCY 2 cut(s) 867, 945
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 4 cut(s) 157, 233, 648, 774
BtgI CCRYGG 1 cut(s) 649
BtsCI GGATG 4 cut(s) 317, 337, 809, 933
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 3 cut(s) 532, 574, 903
CciI TCATGA 1 cut(s) 322
CfoI GCGC 2 cut(s) 223, 398
Cfr10I RCCGGY 1 cut(s) 157
Csp6I GTAC 4 cut(s) 391, 442, 449, 912
CviAII CATG 4 cut(s) 323, 650, 879, 932
CviQI GTAC 4 cut(s) 391, 442, 449, 912
DdeI CTNAG 2 cut(s) 224, 827
DpnI GATC 5 cut(s) 26, 246, 762, 869, 947
DpnII GATC 5 cut(s) 24, 244, 760, 867, 945
EaeI YGGCCR 3 cut(s) 155, 646, 772
EagI CGGCCG 1 cut(s) 155
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 2 cut(s) 281, 843
EclXI CGGCCG 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 649
Eco24I GRGCYC 3 cut(s) 37, 283, 845
Eco47III AGCGCT 1 cut(s) 222
Eco52I CGGCCG 1 cut(s) 155
Eco53kI GAGCTC 2 cut(s) 281, 843
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 36, 276
EcoICRI GAGCTC 2 cut(s) 281, 843
EcoRII CCWGG 3 cut(s) 270, 514, 727
EcoT14I CCWWGG 1 cut(s) 649
EcoT22I ATGCAT 2 cut(s) 889, 933
EcoT38I GRGCYC 3 cut(s) 37, 283, 845
ErhI CCWWGG 1 cut(s) 649
FaeI CATG 4 cut(s) 326, 653, 882, 935
FaqI GGGAC 1 cut(s) 389
FatI CATG 4 cut(s) 322, 649, 878, 931
FauI CCCGC 2 cut(s) 69, 391
FblI GTMKAC 1 cut(s) 480
FokI GGATG 4 cut(s) 324, 324, 796, 940
FriOI GRGCYC 3 cut(s) 37, 283, 845
FspBI CTAG 1 cut(s) 861
GlaI GCGC 2 cut(s) 222, 397
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 4 cut(s) 157, 233, 648, 774
HapII CCGG 3 cut(s) 158, 204, 329
HhaI GCGC 2 cut(s) 223, 398
Hin1II CATG 4 cut(s) 326, 653, 882, 935
Hin6I GCGC 2 cut(s) 221, 396
HinP1I GCGC 2 cut(s) 221, 396
HindIII AAGCTT 1 cut(s) 122
HpaII CCGG 3 cut(s) 158, 204, 329
HphI GGTGA 2 cut(s) 530, 846
Hpy166II GTNNAC 3 cut(s) 261, 481, 779
Hpy188I TCNGA 3 cut(s) 150, 178, 847
Hpy188III TCNNGA 4 cut(s) 74, 215, 323, 821
Hpy8I GTNNAC 3 cut(s) 261, 481, 779
Hpy99I CGWCG 2 cut(s) 182, 196
HpyAV CCTTC 2 cut(s) 867, 918
HpyCH4III ACNGT 4 cut(s) 185, 441, 527, 768
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 5 cut(s) 533, 720, 809, 887, 931
HpyF10VI GCNNNNNNNGC 8 cut(s) 103, 109, 112, 115, 121, 151, 395, 884
HpyF3I CTNAG 2 cut(s) 224, 827
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 4 cut(s) 326, 653, 882, 935
HspAI GCGC 2 cut(s) 221, 396
Kzo9I GATC 5 cut(s) 24, 244, 760, 867, 945
LmnI GCTCC 3 cut(s) 578, 754, 840
Lsp1109I GCAGC 8 cut(s) 115, 118, 121, 124, 127, 151, 154, 576
LweI GCATC 5 cut(s) 154, 520, 601, 818, 918
MaeI CTAG 1 cut(s) 861
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 3 cut(s) 8, 333, 425
MalI GATC 5 cut(s) 26, 246, 762, 869, 947
MboI GATC 5 cut(s) 24, 244, 760, 867, 945
MflI RGATCY 2 cut(s) 867, 945
MhlI GDGCHC 4 cut(s) 37, 283, 759, 845
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 2 cut(s) 238, 815
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 6 cut(s) 182, 313, 422, 545, 628, 672
MmeI TCCRAC 3 cut(s) 201, 635, 845
Mox20I TGGCCA 1 cut(s) 648
Mph1103I ATGCAT 2 cut(s) 889, 933
MroXI GAANNNNTTC 1 cut(s) 50
MscI TGGCCA 1 cut(s) 648
MseI TTAA 2 cut(s) 416, 686
Msp20I TGGCCA 1 cut(s) 648
MspI CCGG 3 cut(s) 158, 204, 329
MspR9I CCNGG 4 cut(s) 205, 272, 516, 729
MvaI CCWGG 3 cut(s) 272, 516, 729
MvnI CGCG 2 cut(s) 236, 398
MwoI GCNNNNNNNGC 8 cut(s) 103, 109, 112, 115, 121, 151, 395, 884
NciI CCSGG 1 cut(s) 205
NcoI CCATGG 1 cut(s) 649
NdeII GATC 5 cut(s) 24, 244, 760, 867, 945
NlaIII CATG 4 cut(s) 326, 653, 882, 935
NlaIV GGNNCC 2 cut(s) 251, 694
NmuCI GTSAC 2 cut(s) 8, 425
NsiI ATGCAT 2 cut(s) 889, 933
NspI RCATGY 1 cut(s) 935
PaeR7I CTCGAG 1 cut(s) 276
PagI TCATGA 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 5 cut(s) 51, 70, 368, 591, 796
Pfl23II CGTACG 1 cut(s) 390
PflFI GACNNNGTC 1 cut(s) 431
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 6 cut(s) 181, 313, 422, 544, 627, 671
PpsI GAGTC 6 cut(s) 181, 313, 422, 544, 627, 671
Psp124BI GAGCTC 2 cut(s) 283, 845
Psp6I CCWGG 3 cut(s) 270, 514, 727
PspGI CCWGG 3 cut(s) 270, 514, 727
PspLI CGTACG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 251, 694
PspXI VCTCGAGB 1 cut(s) 276
PsrI GAACNNNNNNTAC 2 cut(s) 38, 70
PsuI RGATCY 2 cut(s) 867, 945
PsyI GACNNNGTC 1 cut(s) 431
PvuI CGATCG 1 cut(s) 27
RsaI GTAC 4 cut(s) 392, 443, 450, 913
RsaNI GTAC 4 cut(s) 391, 442, 449, 912
SacI GAGCTC 2 cut(s) 283, 845
SaqAI TTAA 2 cut(s) 416, 686
Sau3AI GATC 5 cut(s) 24, 244, 760, 867, 945
ScaI AGTACT 1 cut(s) 443
SchI GAGTC 6 cut(s) 182, 313, 422, 545, 628, 672
ScrFI CCNGG 4 cut(s) 205, 272, 516, 729
SduI GDGCHC 4 cut(s) 37, 283, 759, 845
SfaNI GCATC 5 cut(s) 154, 520, 601, 818, 918
SfcI CTRYAG 1 cut(s) 873
Sfr274I CTCGAG 1 cut(s) 276
SlaI CTCGAG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 254, 276, 697
SmoI CTYRAG 3 cut(s) 254, 276, 697
Sse9I AATT 2 cut(s) 238, 815
SsiI CCGC 5 cut(s) 15, 62, 154, 234, 398
SspI AATATT 2 cut(s) 631, 749
SspMI CTAG 1 cut(s) 861
SstI GAGCTC 2 cut(s) 283, 845
StyD4I CCNGG 4 cut(s) 203, 270, 514, 727
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 4 cut(s) 185, 441, 527, 768
TaiI ACGT 1 cut(s) 194
TaqI TCGA 4 cut(s) 23, 73, 277, 542
TaqII GACCGA 1 cut(s) 17
TasI AATT 2 cut(s) 238, 815
TatI WGTACW 2 cut(s) 441, 911
TauI GCSGC 2 cut(s) 157, 236
TfiI GAWTC 5 cut(s) 51, 70, 368, 591, 796
Tru1I TTAA 2 cut(s) 416, 686
Tru9I TTAA 2 cut(s) 416, 686
TscAI CASTG 3 cut(s) 532, 574, 910
TseFI GTSAC 2 cut(s) 8, 425
TseI GCWGC 8 cut(s) 103, 106, 109, 112, 115, 139, 142, 564
Tsp45I GTSAC 2 cut(s) 8, 425
TspDTI ATGAA 1 cut(s) 720
TspRI CASTG 3 cut(s) 532, 574, 910
Tth111I GACNNNGTC 1 cut(s) 431
XapI RAATTY 1 cut(s) 238
XceI RCATGY 1 cut(s) 935
XhoI CTCGAG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 1 cut(s) 861
ZrmI AGTACT 1 cut(s) 443
Zsp2I ATGCAT 2 cut(s) 889, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.