MD13G1229200.v1.1

GDT1-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007177
Physical Location & Seq
Forward (+)
22472206 .. 22473269
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1229200.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGTGATAAAAGCAGCCCCTGAGCTTGATGAATACACCGAAGCCGAGGAGCTTGTGAAAGAGAAGGTGTCAAAGCGGCTCACTAATCCATTTGAAATTGTCTGGAAATCATTCACCCTCGTATTTGTTGCCGAATGGGGAGATCGCTCAATGCTCGCCACAATCGCTCTTGGTGCTGCACAGTCTCCATGGGGTGTGGCAAGTGGAGCCACTGCTGGACACGTACTTGCGACATCTATCGCTGTTCTAGGAGGTGCATTTCTCGCCAATTACATTTCCGAAAAACTGGTTGGATATGTGGGAGGTGGGTTGTTTCTAGTTTTTGCGGTAGCCACATTTTTCGGAGTTTTCTGA

Protein Analysis

118

Amino Acids

12.43

Weight (kDa)

5.03

Isoelectric Point (pI)

26.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 35 - 109 9.4e-25 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 76, 326
AfaI GTAC 1 cut(s) 225
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 95
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 2 cut(s) 25, 52
AluI AGCT 2 cut(s) 25, 52
Alw26I GTCTC 1 cut(s) 189
AlwNI CAGNNNCTG 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 14, 176
Asp700I GAANNNNTTC 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 16, 106
BbvI GCAGC 2 cut(s) 26, 163
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 2 cut(s) 248, 317
BisI GCNGC 3 cut(s) 15, 77, 177
BlsI GCNGC 3 cut(s) 16, 78, 178
BmiI GGNNCC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 45, 188
Bse1I ACTGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 45, 188
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 26, 163
BsgI GTGCAG 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 142
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 2 cut(s) 76, 326
BspCNI CTCAG 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 208
BsrI ACTGG 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 45, 188
BssMI GATC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 183
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 21
BstDSI CCRYGG 1 cut(s) 188
BstKTI GATC 1 cut(s) 145
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 4 cut(s) 164, 173, 206, 263
BstV1I GCAGC 2 cut(s) 26, 163
BtgI CCRYGG 1 cut(s) 188
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 156
CaiI CAGNNNCTG 1 cut(s) 20
Csp6I GTAC 1 cut(s) 224
CviAII CATG 1 cut(s) 189
CviJI RGCY 7 cut(s) 17, 25, 44, 52, 79, 209, 332
CviKI_1 RGCY 7 cut(s) 17, 25, 44, 52, 79, 209, 332
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 1 cut(s) 21
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 1 cut(s) 192
FaiI YATR 2 cut(s) 190, 297
FatI CATG 1 cut(s) 188
Fnu4HI GCNGC 3 cut(s) 15, 77, 177
Fsp4HI GCNGC 3 cut(s) 15, 77, 177
FspBI CTAG 2 cut(s) 248, 317
GluI GCNGC 3 cut(s) 15, 77, 177
Hin1II CATG 1 cut(s) 192
HphI GGTGA 2 cut(s) 16, 106
Hpy188I TCNGA 3 cut(s) 280, 344, 353
Hpy188III TCNNGA 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 179, 257
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 173, 206, 263
HpyF3I CTNAG 1 cut(s) 21
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 192
Kzo9I GATC 1 cut(s) 142
LmnI GCTCC 2 cut(s) 49, 206
LpnPI CCDG 4 cut(s) 33, 88, 201, 272
Lsp1109I GCAGC 2 cut(s) 26, 163
MaeI CTAG 2 cut(s) 248, 317
MaeII ACGT 1 cut(s) 222
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MluCI AATT 2 cut(s) 96, 268
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 4 cut(s) 40, 128, 245, 296
MroXI GAANNNNTTC 1 cut(s) 110
MwoI GCNNNNNNNGC 4 cut(s) 164, 173, 206, 263
NcoI CCATGG 1 cut(s) 188
NdeII GATC 1 cut(s) 142
NlaIII CATG 1 cut(s) 192
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 16, 78, 178
Ppu21I YACGTR 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 20
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SatI GCNGC 3 cut(s) 15, 77, 177
Sau3AI GATC 1 cut(s) 142
SetI ASST 6 cut(s) 27, 54, 69, 225, 256, 307
Sse9I AATT 2 cut(s) 96, 268
SsiI CCGC 2 cut(s) 76, 326
SspMI CTAG 2 cut(s) 248, 317
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 183
TaiI ACGT 1 cut(s) 225
TasI AATT 2 cut(s) 96, 268
TauI GCSGC 1 cut(s) 79
TscAI CASTG 1 cut(s) 217
TseI GCWGC 2 cut(s) 14, 176
TspDTI ATGAA 1 cut(s) 45
TspRI CASTG 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 2 cut(s) 248, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.