MD16G1235100.v1.1

GDT1-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007177
Physical Location & Seq
Forward (+)
24185605 .. 24187224
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1235100.v1.1.491

Sequence Viewer

Length: 516 bp
ATGACAGTCCTGTCTGTCATTATTGGGCGAATATTTAACTCGGTCCCTGCTCAATTCCAGACAACCTTGCCAATTGGAGAATATGCAGCAGTAACTCTTCTACTATTCTTCGGTCTCAAATCAATAAAGGATGCATGGGACCTTCCATCAAGTGATGTGAAGAGTGGTGATAAGAGCAGCCCTGAGCTCGGTGAATATACCGAGGCCGAGGAGCTTGTGAAAGAGAAGGTGTCAAAGCGGCTCACTAATCCACTTGAAATTGTCTGGAAATCGTTCACCCTTGTATTCGTTGCCGAATGGGGAGATCGCTCAATGCTCGCCACGATCGCTCTCGGTGCTGCGCAGTCTCCATGGGGCGTGGCAAGTGGAGCCATTGCTGGACACGTACTTGCGACATCTATTGCTGTTCTAGGAGGGGCATTTCTTGCCAACTACATTTCCGAAAAACTGGTTGGCTACGTGGGAGGTGGGTTGTTCCTAGTTTTTGCAGTAGCCACATTTTTCGGAGTTTTCTGA

Protein Analysis

172

Amino Acids

18.2

Weight (kDa)

5.13

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 89 - 163 3.6e-24 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc16I TGCGCA 1 cut(s) 342
AciI CCGC 1 cut(s) 238
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 382
AgsI TTSAA 1 cut(s) 257
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 2 cut(s) 187, 214
AluI AGCT 2 cut(s) 187, 214
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 119, 351
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 3 cut(s) 86, 177, 338
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 43, 139
AsuHPI GGTGA 3 cut(s) 179, 203, 268
AvaII GGWCC 2 cut(s) 43, 139
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 98, 189, 325
BccI CCATC 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 119, 351
BfaI CTAG 2 cut(s) 410, 479
BisI GCNGC 4 cut(s) 87, 178, 239, 339
BlsI GCNGC 4 cut(s) 88, 179, 240, 340
Bme18I GGWCC 2 cut(s) 43, 139
BmgT120I GGNCC 2 cut(s) 43, 139
BmiI GGNNCC 3 cut(s) 45, 140, 370
BmsI GCATC 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 183
BsaAI YACGTR 2 cut(s) 385, 460
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 3 cut(s) 201, 207, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 201, 207, 350
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 98, 189, 325
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 189
BslFI GGGAC 2 cut(s) 29, 152
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 2 cut(s) 119, 351
BsmFI GGGAC 2 cut(s) 29, 152
BsnI GGCC 1 cut(s) 206
Bso31I GGTCTC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 2 cut(s) 304, 324
Bsp19I CCATGG 1 cut(s) 350
BspACI CCGC 1 cut(s) 238
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 3 cut(s) 45, 140, 370
BspTNI GGTCTC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 453
BssECI CCNNGG 3 cut(s) 201, 207, 350
BssMI GATC 2 cut(s) 304, 324
BssT1I CCWWGG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 7
Bst6I CTCTTC 2 cut(s) 102, 155
BstAPI GCANNNNNTGC 1 cut(s) 425
BstBAI YACGTR 2 cut(s) 385, 460
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 1 cut(s) 183
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 136
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 2 cut(s) 307, 327
BstMAI GTCTC 2 cut(s) 119, 351
BstMBI GATC 2 cut(s) 304, 324
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 4 cut(s) 326, 335, 368, 425
BstV1I GCAGC 3 cut(s) 98, 189, 325
BsuRI GGCC 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 350
BtsCI GGATG 1 cut(s) 136
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 43, 139
Csp6I GTAC 1 cut(s) 386
CviAII CATG 2 cut(s) 135, 351
CviJI RGCY 8 cut(s) 180, 187, 206, 214, 241, 371, 456, 494
CviKI_1 RGCY 8 cut(s) 180, 187, 206, 214, 241, 371, 456, 494
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 1 cut(s) 183
DpnI GATC 2 cut(s) 306, 326
DpnII GATC 2 cut(s) 304, 324
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 102, 155
EarI CTCTTC 2 cut(s) 102, 155
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 119
Eco47I GGWCC 2 cut(s) 43, 139
Eco53kI GAGCTC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 350
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 350
FaeI CATG 2 cut(s) 138, 354
FaiI YATR 4 cut(s) 84, 136, 198, 352
FaqI GGGAC 2 cut(s) 29, 152
FatI CATG 2 cut(s) 134, 350
Fnu4HI GCNGC 4 cut(s) 87, 178, 239, 339
FokI GGATG 1 cut(s) 143
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 4 cut(s) 87, 178, 239, 339
FspBI CTAG 2 cut(s) 410, 479
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 4 cut(s) 87, 178, 239, 339
HaeIII GGCC 1 cut(s) 206
HhaI GCGC 1 cut(s) 343
Hin1II CATG 2 cut(s) 138, 354
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HphI GGTGA 3 cut(s) 179, 203, 268
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 3 cut(s) 442, 506, 515
Hpy188III TCNNGA 2 cut(s) 58, 265
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 152, 220
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 2 cut(s) 384, 459
HpyCH4V TGCA 3 cut(s) 86, 134, 488
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 335, 368, 425
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 2 cut(s) 384, 459
Hsp92II CATG 2 cut(s) 138, 354
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 2 cut(s) 304, 324
LmnI GCTCC 2 cut(s) 211, 368
LpnPI CCDG 7 cut(s) 23, 60, 71, 195, 250, 363, 434
Lsp1109I GCAGC 3 cut(s) 98, 189, 325
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 410, 479
MaeII ACGT 2 cut(s) 384, 459
MaeIII GTNAC 1 cut(s) 91
MalI GATC 2 cut(s) 306, 326
MboI GATC 2 cut(s) 304, 324
MboII GAAGA 3 cut(s) 89, 100, 172
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 3 cut(s) 53, 72, 258
MnlI CCTC 4 cut(s) 196, 202, 407, 458
Mph1103I ATGCAT 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 1 cut(s) 36
MunI CAATTG 1 cut(s) 72
MwoI GCNNNNNNNGC 4 cut(s) 326, 335, 368, 425
NcoI CCATGG 1 cut(s) 350
NdeII GATC 2 cut(s) 304, 324
NlaIII CATG 2 cut(s) 138, 354
NlaIV GGNNCC 3 cut(s) 45, 140, 370
NmeAIII GCCGAG 1 cut(s) 232
NsbI TGCGCA 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 88, 179, 240, 340
Ple19I CGATCG 1 cut(s) 327
Ppu21I YACGTR 2 cut(s) 385, 460
PpuMI RGGWCCY 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 45, 140, 370
PspPI GGNCC 2 cut(s) 43, 139
PspPPI RGGWCCY 1 cut(s) 139
PvuI CGATCG 1 cut(s) 327
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 4 cut(s) 87, 178, 239, 339
Sau3AI GATC 2 cut(s) 304, 324
Sau96I GGNCC 2 cut(s) 43, 139
SduI GDGCHC 1 cut(s) 189
SetI ASST 8 cut(s) 68, 144, 189, 216, 231, 387, 462, 469
SfaNI GCATC 1 cut(s) 121
SinI GGWCC 2 cut(s) 43, 139
Sse9I AATT 3 cut(s) 53, 72, 258
SsiI CCGC 1 cut(s) 238
SspI AATATT 1 cut(s) 33
SspMI CTAG 2 cut(s) 410, 479
SstI GAGCTC 1 cut(s) 189
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 2 cut(s) 387, 462
TaqII GACCGA 2 cut(s) 31, 101
TasI AATT 3 cut(s) 53, 72, 258
TauI GCSGC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TseI GCWGC 3 cut(s) 86, 177, 338
VpaK11BI GGWCC 2 cut(s) 43, 139
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 2 cut(s) 410, 479
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.