MD16G1235000.v1.1

GDT1-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007177
Physical Location & Seq
Forward (+)
24183537 .. 24185603
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1235000.v1.1.491

Sequence Viewer

Length: 564 bp
ATGCAAGGACTGGGGATTCTCAATACACCAATGCCATTCAATCCAAGCAAGAAGCTTCCCTTGTCAGCTTTTACGGACGCTCTTCCAAGCTGTCCCACTCTTTCCAGAAAACCATCGTTGAGATGTAGAGTGATATCAAGGTGTGCTGGCAGAAACGTACGAGTCCAAGCATCTAATGTTAGTATCGGATCTGGAGGATATGAAGACAGTGAGGAAAACAAAAACAAGAAAATATTTCCAAGTGATGCACCCAGTGACAGTTCATCTGAAATTGAGAAGCATCCAGTTCAAATCCCTTATCCCCTCTCTGTGGGTCTTGTGCCACTTGGGTGCGCCTTAGTTTTTGCTCTGATTGCCTTTGTGAAAGGAGGACCTTCAGCACTTTTAGCAGCAATTGCAAAATCAGGCTTTACAGCTGCATTTTCTTTAATATTTGTTTCTGAAATTGGTGACAAGACATTCTTCATTGCTGCACTCCTAGCCATGCAATATGATAAAGTACTGGGGAGGCGGGAGTTTCAAACCTGGGAGGCATGGGTTGAAACCCAACACCTTATCCACTAA

Protein Analysis

188

Amino Acids

20.21

Weight (kDa)

8.95

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 139 - 166 7.4e-09 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 511
AclWI GGATC 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 360
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 2 cut(s) 159, 501
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 4 cut(s) 40, 290, 521, 542
AjnI CCWGG 1 cut(s) 524
AleI CACNNNNGTG 1 cut(s) 328
AluBI AGCT 4 cut(s) 55, 68, 90, 416
AluI AGCT 4 cut(s) 55, 68, 90, 416
AlwI GGATC 1 cut(s) 196
ApeKI GCWGC 3 cut(s) 389, 416, 470
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 461
AvaII GGWCC 1 cut(s) 371
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 401, 403, 457
BccI CCATC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 526
BfaI CTAG 1 cut(s) 479
BisI GCNGC 3 cut(s) 390, 417, 471
BlsI GCNGC 3 cut(s) 391, 418, 472
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 526
BmrI ACTGGG 3 cut(s) 20, 246, 512
BmsI GCATC 3 cut(s) 179, 235, 289
BmuI ACTGGG 3 cut(s) 20, 246, 512
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse1I ACTGG 4 cut(s) 15, 252, 284, 507
Bse3DI GCAATG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 526
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMI GCAATG 1 cut(s) 465
BseNI ACTGG 4 cut(s) 15, 252, 284, 507
BseXI GCAGC 3 cut(s) 401, 403, 457
BsgI GTGCAG 1 cut(s) 456
BsiWI CGTACG 1 cut(s) 157
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 188
BspACI CCGC 1 cut(s) 511
BspPI GGATC 1 cut(s) 196
BspQI GCTCTTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 465
BsrI ACTGG 4 cut(s) 15, 252, 284, 507
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 526
Bst4CI ACNGT 2 cut(s) 209, 260
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 1 cut(s) 337
BstF5I GGATG 1 cut(s) 280
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 1 cut(s) 191
BstMBI GATC 1 cut(s) 188
BstMWI GCNNNNNNNGC 4 cut(s) 353, 386, 395, 479
BstNI CCWGG 1 cut(s) 526
BstSCI CCNGG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 401, 403, 457
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 214, 259
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 158, 500
CviAII CATG 2 cut(s) 484, 534
CviJI RGCY 6 cut(s) 55, 68, 90, 408, 416, 482
CviKI_1 RGCY 6 cut(s) 55, 68, 90, 408, 416, 482
CviQI GTAC 2 cut(s) 158, 500
DdeI CTNAG 1 cut(s) 337
DpnI GATC 1 cut(s) 190
DpnII GATC 1 cut(s) 188
DraIII CACNNNGTG 1 cut(s) 254
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 360
EcoO109I RGGNCCY 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 524
EcoRV GATATC 1 cut(s) 135
FaeI CATG 2 cut(s) 487, 537
FaiI YATR 4 cut(s) 201, 485, 492, 535
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 446, 478
FaqI GGGAC 1 cut(s) 78
FatI CATG 2 cut(s) 483, 533
FauI CCCGC 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 390, 417, 471
FokI GGATG 1 cut(s) 267
Fsp4HI GCNGC 3 cut(s) 390, 417, 471
FspBI CTAG 1 cut(s) 479
GlaI GCGC 1 cut(s) 334
GluI GCNGC 3 cut(s) 390, 417, 471
GsuI CTGGAG 1 cut(s) 213
HgaI GACGC 1 cut(s) 86
HhaI GCGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 487, 537
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 16, 162
HphI GGTGA 1 cut(s) 461
Hpy188I TCNGA 4 cut(s) 188, 268, 351, 442
Hpy188III TCNNGA 2 cut(s) 105, 192
HpyAV CCTTC 1 cut(s) 384
HpyCH4III ACNGT 2 cut(s) 209, 260
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 6 cut(s) 4, 248, 398, 419, 473, 487
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 386, 395, 479
HpyF3I CTNAG 1 cut(s) 337
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 2 cut(s) 487, 537
HspAI GCGC 1 cut(s) 333
Kzo9I GATC 1 cut(s) 188
LguI GCTCTTC 1 cut(s) 87
LpnPI CCDG 9 cut(s) 118, 132, 177, 265, 297, 390, 488, 511, 538
Lsp1109I GCAGC 3 cut(s) 401, 403, 457
LweI GCATC 3 cut(s) 179, 235, 289
MaeI CTAG 1 cut(s) 479
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 254, 449
MalI GATC 1 cut(s) 190
MboI GATC 1 cut(s) 188
MboII GAAGA 3 cut(s) 74, 215, 454
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 1 cut(s) 188
MluCI AATT 3 cut(s) 270, 393, 444
MlyI GAGTC 1 cut(s) 171
MnlI CCTC 6 cut(s) 188, 205, 314, 362, 501, 523
MseI TTAA 1 cut(s) 428
MslI CAYNNNNRTG 1 cut(s) 328
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 526
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 526
MwoI GCNNNNNNNGC 4 cut(s) 353, 386, 395, 479
NdeII GATC 1 cut(s) 188
NlaIII CATG 2 cut(s) 487, 537
NmuCI GTSAC 2 cut(s) 254, 449
OliI CACNNNNGTG 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 87
PfeI GAWTC 1 cut(s) 16
Pfl23II CGTACG 1 cut(s) 157
PkrI GCNGC 3 cut(s) 391, 418, 472
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PpuMI RGGWCCY 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 524
PspGI CCWGG 1 cut(s) 524
PspLI CGTACG 1 cut(s) 157
PspPI GGNCC 1 cut(s) 371
PspPPI RGGWCCY 1 cut(s) 371
PsuI RGATCY 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 159, 501
RsaNI GTAC 2 cut(s) 158, 500
RseI CAYNNNNRTG 1 cut(s) 328
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 428
SatI GCNGC 3 cut(s) 390, 417, 471
Sau3AI GATC 1 cut(s) 188
Sau96I GGNCC 1 cut(s) 371
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 526
SetI ASST 9 cut(s) 57, 70, 92, 143, 159, 376, 418, 527, 555
SfaNI GCATC 3 cut(s) 179, 235, 289
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 328
Sse9I AATT 3 cut(s) 270, 393, 444
SsiI CCGC 1 cut(s) 511
SspI AATATT 2 cut(s) 234, 432
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 524
TaaI ACNGT 2 cut(s) 209, 260
TaiI ACGT 1 cut(s) 159
TasI AATT 3 cut(s) 270, 393, 444
TatI WGTACW 1 cut(s) 499
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 428
Tru9I TTAA 1 cut(s) 428
TscAI CASTG 2 cut(s) 214, 259
TseFI GTSAC 2 cut(s) 254, 449
TseI GCWGC 3 cut(s) 389, 416, 470
Tsp45I GTSAC 2 cut(s) 254, 449
TspDTI ATGAA 3 cut(s) 216, 252, 454
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 2 cut(s) 214, 259
VpaK11BI GGWCC 1 cut(s) 371
XspI CTAG 1 cut(s) 479
ZrmI AGTACT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.