MD13G1256300.v1.1

Yip1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006639
Physical Location & Seq
Reverse (-)
28066295 .. 28068276
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1256300.v1.1.491

Sequence Viewer

Length: 840 bp
ATGTCGCACTCCGAACGCGACACCGTCCCCCTCCACCCCTCATCCCAATCCGACATCGACGAGATCGAAAACCTAATCAACGCCAGCGTCCAATCGGGCCCCACCACCGTCCTCCCCGCCAAACCCCCCAGTCCTCCCCGCGCCTCCATTCCCGTCTCATCATCCCCTTTCATCCCATCAAACCTCCCGCCCCCAGCCCCCTCCTCCGCCGTGAAGCAGAAGCCTCCGTCCGTCCCCGCCCCACCTCCCATTCCCTCCGCAACCAACGGCCCCAGTATCTCCCCCTCCGGGTTCGGCTCCCCGCCCAACACCCTAACCGAGCCCGTTTGGGACACCGTGAAGCGCGACTTGTCGAGAATCGTGAGCAATTTGAAGCTTGTGGTGTTCCCTAACCCGTATCGTGAGGATCCGGGAAAGGCCTTGAGGGATTGGGATCTCTGGGGACCCTTCTTCTTCATTGTATTCTTGGGTCTCACTCTTTCCTGGTCTGCATCTGTCAGAAAGTCTGAGGTTTTTGCTGTTGCTTTTGCTGTGCTTGCTGCTGGTGCTGTGATTCTGACACTGAATGTACTCCTACTGGGTGGACACATAATATTCTTCCAGAGCCTTAGTCTTCTCGGTTATTGCTTATTTCCTCTGGACGTTGGAGCTCTGATATGTATGGTGAAGGATTATGTGATATTGAAGGTGGTGGTGGTAAGCGTAACACTGGCTTGGAGTTCATGGGCTGCATATCCTTTCATGAGTTCAGCAGTCAACCCCAGGAGAAAAGCCCTTGCACTTTACCCTGTTTTCCTATTGTATGTATCCGTCGGTTTCCTTATTATCGCTATTGATTGA

Protein Analysis

280

Amino Acids

29.95

Weight (kDa)

8.53

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yip1 PF04893 141 - 273 2.8e-15 Yip1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 18, 141, 345
AciI CCGC 7 cut(s) 117, 139, 188, 207, 237, 258, 302
AclWI GGATC 3 cut(s) 401, 414, 441
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 2 cut(s) 373, 685
AjnI CCWGG 2 cut(s) 482, 761
AluBI AGCT 2 cut(s) 376, 650
AluI AGCT 2 cut(s) 376, 650
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 2 cut(s) 160, 476
AlwI GGATC 3 cut(s) 401, 414, 441
AoxI GGCC 3 cut(s) 97, 268, 417
ApaI GGGCCC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 539, 728
AspLEI GCGC 2 cut(s) 143, 345
AspS9I GGNCC 4 cut(s) 97, 98, 269, 443
AsuC2I CCSGG 2 cut(s) 289, 411
AsuHPI GGTGA 1 cut(s) 676
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 101
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 3 cut(s) 101, 324, 652
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 2 cut(s) 526, 715
BccI CCATC 1 cut(s) 184
BceAI ACGGC 2 cut(s) 194, 283
BciT130I CCWGG 2 cut(s) 484, 763
BciVI GTATCC 1 cut(s) 817
BcnI CCSGG 2 cut(s) 289, 411
BcoDI GTCTC 2 cut(s) 160, 476
BfuI GTATCC 1 cut(s) 817
BisI GCNGC 2 cut(s) 540, 729
BlsI GCNGC 2 cut(s) 541, 730
Bme1390I CCNGG 4 cut(s) 289, 411, 484, 763
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 4 cut(s) 97, 98, 269, 443
BmiI GGNNCC 7 cut(s) 99, 100, 271, 298, 408, 444, 445
BmrFI CCNGG 4 cut(s) 289, 411, 484, 763
BmrI ACTGGG 3 cut(s) 123, 267, 587
BmsI GCATC 1 cut(s) 500
BmuI ACTGGG 3 cut(s) 123, 267, 587
BpiI GAAGAC 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 442
BpuMI CCSGG 2 cut(s) 289, 411
BsaBI GATNNNNATC 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 761
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 4 cut(s) 129, 273, 582, 714
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 2 cut(s) 484, 763
BseDI CCNNGG 1 cut(s) 761
BseGI GGATG 3 cut(s) 41, 161, 171
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 4 cut(s) 129, 273, 582, 714
BseRI GAGGAG 1 cut(s) 193
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 2 cut(s) 526, 715
BseYI CCCAGC 1 cut(s) 193
Bsh1236I CGCG 3 cut(s) 18, 141, 345
BshFI GGCC 3 cut(s) 99, 270, 419
BsiHKAI GWGCWC 1 cut(s) 652
BsiSI CCGG 2 cut(s) 288, 410
BslFI GGGAC 4 cut(s) 11, 218, 344, 456
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 2 cut(s) 160, 476
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 4 cut(s) 11, 218, 344, 456
BsnI GGCC 3 cut(s) 99, 270, 419
Bso31I GGTCTC 1 cut(s) 476
Bsp120I GGGCCC 1 cut(s) 97
Bsp1286I GDGCHC 3 cut(s) 101, 324, 652
Bsp143I GATC 3 cut(s) 63, 406, 433
BspACI CCGC 7 cut(s) 117, 139, 188, 207, 237, 258, 302
BspANI GGCC 3 cut(s) 99, 270, 419
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 3 cut(s) 18, 141, 345
BspHI TCATGA 1 cut(s) 741
BspLI GGNNCC 7 cut(s) 99, 100, 271, 298, 408, 444, 445
BspPI GGATC 3 cut(s) 401, 414, 441
BspTNI GGTCTC 1 cut(s) 476
BsrI ACTGG 4 cut(s) 129, 273, 582, 714
BssECI CCNNGG 1 cut(s) 761
BssMI GATC 3 cut(s) 63, 406, 433
Bst2UI CCWGG 2 cut(s) 484, 763
Bst4CI ACNGT 3 cut(s) 25, 109, 337
BstC8I GCNNGC 2 cut(s) 85, 537
BstDEI CTNAG 2 cut(s) 507, 608
BstF5I GGATG 3 cut(s) 41, 161, 171
BstFNI CGCG 3 cut(s) 18, 141, 345
BstHHI GCGC 2 cut(s) 143, 345
BstKTI GATC 3 cut(s) 66, 409, 436
BstMAI GTCTC 2 cut(s) 160, 476
BstMBI GATC 3 cut(s) 63, 406, 433
BstMWI GCNNNNNNNGC 2 cut(s) 536, 545
BstNI CCWGG 2 cut(s) 484, 763
BstSCI CCNGG 4 cut(s) 287, 409, 482, 761
BstSLI GKGCMC 1 cut(s) 101
BstUI CGCG 3 cut(s) 18, 141, 345
BstV1I GCAGC 2 cut(s) 526, 715
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 406, 433
BstYI RGATCY 2 cut(s) 406, 433
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 3 cut(s) 99, 270, 419
BtsCI GGATG 3 cut(s) 41, 161, 171
BtsIMutI CAGTG 2 cut(s) 560, 707
Cac8I GCNNGC 2 cut(s) 85, 537
CciI TCATGA 1 cut(s) 741
CfoI GCGC 2 cut(s) 143, 345
Cfr13I GGNCC 4 cut(s) 97, 98, 269, 443
CseI GACGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 569
CviAII CATG 2 cut(s) 723, 742
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 2 cut(s) 507, 608
DpnI GATC 3 cut(s) 65, 408, 435
DpnII GATC 3 cut(s) 63, 406, 433
EciI GGCGGA 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 650
Eco147I AGGCCT 1 cut(s) 419
Eco24I GRGCYC 3 cut(s) 101, 324, 652
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 443
Eco53kI GAGCTC 1 cut(s) 650
EcoICRI GAGCTC 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 98, 443
EcoRII CCWGG 2 cut(s) 482, 761
EcoT38I GRGCYC 3 cut(s) 101, 324, 652
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 2 cut(s) 726, 745
FaiI YATR 8 cut(s) 590, 658, 662, 675, 724, 733, 743, 804
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FaqI GGGAC 4 cut(s) 11, 218, 344, 456
FatI CATG 2 cut(s) 722, 741
FauI CCCGC 5 cut(s) 124, 146, 195, 244, 309
Fnu4HI GCNGC 2 cut(s) 540, 729
FokI GGATG 3 cut(s) 28, 148, 158
FriOI GRGCYC 3 cut(s) 101, 324, 652
Fsp4HI GCNGC 2 cut(s) 540, 729
GlaI GCGC 2 cut(s) 142, 344
GluI GCNGC 2 cut(s) 540, 729
GsaI CCCAGC 1 cut(s) 197
HaeIII GGCC 3 cut(s) 99, 270, 419
HapII CCGG 2 cut(s) 288, 410
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 143, 345
Hin1II CATG 2 cut(s) 726, 745
Hin6I GCGC 2 cut(s) 141, 343
HinP1I GCGC 2 cut(s) 141, 343
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 357, 553
HpaII CCGG 2 cut(s) 288, 410
HphI GGTGA 1 cut(s) 676
Hpy166II GTNNAC 2 cut(s) 584, 757
Hpy188I TCNGA 6 cut(s) 13, 52, 500, 508, 558, 654
Hpy188III TCNNGA 6 cut(s) 354, 361, 401, 601, 638, 742
Hpy8I GTNNAC 2 cut(s) 584, 757
Hpy99I CGWCG 2 cut(s) 62, 815
HpyAV CCTTC 3 cut(s) 457, 661, 679
HpyCH4III ACNGT 3 cut(s) 25, 109, 337
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 3 cut(s) 491, 731, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 545
HpyF3I CTNAG 2 cut(s) 507, 608
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 2 cut(s) 726, 745
HspAI GCGC 2 cut(s) 141, 343
KflI GGGWCCC 1 cut(s) 443
Kzo9I GATC 3 cut(s) 63, 406, 433
LmnI GCTCC 2 cut(s) 302, 647
Lsp1109I GCAGC 2 cut(s) 526, 715
LweI GCATC 1 cut(s) 500
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 1 cut(s) 703
MalI GATC 3 cut(s) 65, 408, 435
MboI GATC 3 cut(s) 63, 406, 433
MboII GAAGA 4 cut(s) 442, 445, 589, 605
MflI RGATCY 2 cut(s) 406, 433
MhlI GDGCHC 3 cut(s) 101, 324, 652
MluCI AATT 1 cut(s) 367
MmeI TCCRAC 2 cut(s) 75, 625
MspI CCGG 2 cut(s) 288, 410
MspR9I CCNGG 4 cut(s) 289, 411, 484, 763
MvaI CCWGG 2 cut(s) 484, 763
MvnI CGCG 3 cut(s) 18, 141, 345
MwoI GCNNNNNNNGC 2 cut(s) 536, 545
NciI CCSGG 2 cut(s) 289, 411
NdeII GATC 3 cut(s) 63, 406, 433
NlaIII CATG 2 cut(s) 726, 745
NlaIV GGNNCC 7 cut(s) 99, 100, 271, 298, 408, 444, 445
PagI TCATGA 1 cut(s) 741
PceI AGGCCT 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 357, 553
PflFI GACNNNGTC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 2 cut(s) 541, 730
PpuMI RGGWCCY 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 652
Psp5II RGGWCCY 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 482, 761
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 482, 761
PspN4I GGNNCC 7 cut(s) 99, 100, 271, 298, 408, 444, 445
PspOMI GGGCCC 1 cut(s) 97
PspPI GGNCC 4 cut(s) 97, 98, 269, 443
PspPPI RGGWCCY 1 cut(s) 443
PsuI RGATCY 2 cut(s) 406, 433
PsyI GACNNNGTC 1 cut(s) 23
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SacI GAGCTC 1 cut(s) 652
SatI GCNGC 2 cut(s) 540, 729
Sau3AI GATC 3 cut(s) 63, 406, 433
Sau96I GGNCC 4 cut(s) 97, 98, 269, 443
ScrFI CCNGG 4 cut(s) 289, 411, 484, 763
SduI GDGCHC 3 cut(s) 101, 324, 652
SetI ASST 8 cut(s) 75, 186, 247, 378, 513, 645, 652, 690
SfaNI GCATC 1 cut(s) 500
SinI GGWCC 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 1 cut(s) 367
SseBI AGGCCT 1 cut(s) 419
SsiI CCGC 7 cut(s) 117, 139, 188, 207, 237, 258, 302
SspI AATATT 1 cut(s) 594
SstI GAGCTC 1 cut(s) 652
StuI AGGCCT 1 cut(s) 419
StyD4I CCNGG 4 cut(s) 287, 409, 482, 761
TaaI ACNGT 3 cut(s) 25, 109, 337
TaiI ACGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 57, 66, 353
TasI AATT 1 cut(s) 367
TatI WGTACW 1 cut(s) 568
TfiI GAWTC 2 cut(s) 357, 553
TscAI CASTG 2 cut(s) 567, 714
TseI GCWGC 2 cut(s) 539, 728
TspDTI ATGAA 4 cut(s) 160, 445, 711, 730
TspGWI ACGGA 3 cut(s) 216, 220, 799
TspRI CASTG 2 cut(s) 567, 714
Tth111I GACNNNGTC 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.