Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001332

Physical Location & Seq

Genomic Coordinates Reverse (-)

2934432 .. 2935548

Gene Structure 1117 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1032900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 474 bp

ATGGAAATTTTCACTTTCTTCACCTCCTTGTTACTGTCATCAGCTGCTGCTGCTACACTGTTTGATCAGCATTCTTGTGCTACCGGAGATGGACCAGAGCTAGAGTCGCTGACTCGAGAGCTGCTGGCTTCGGCGATGGAGCCCGAGTTTTTCGAATGGATGAGAGGGTTGAAGAGGAGGATCCACCAGTACCCGGAGCTGGGATTCCAGGAGCACAGGACGAGTGAACTCGTCAGGACCGAGCTCGACTCGCTGGGAGTTGGGGTACAAGTGGCCTTCTTTGCTCTTAGAGCTGACATGGATGCCCTCCCTCTCCAGGAATTGGTAGAGTGGGATTACAAGAGCAAAGTTAATGGAAAGATGCATGCTTGTGGTCATGATTCTCATGTCGCAATGCTACTCGGAGCAGCCAAGTTGCTTCAAAACAAAAAAGATTTGTTGAAGGTGGACGAAAAGCTACATATTTTGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010210 GO:0010211 GO:0010817 GO:0012505 GO:0016787 GO:0016810 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

158

Amino Acids

17.74

Weight (kDa)

5.45

Isoelectric Point (pI)

46.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	95 - 157	6.5e-15	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02875 AT3G02875 AT3G02875
fragaria_vesca	FvH4_6g19670 FvH4_6g19670 FvH4_6g19670 FvH4_6g19670 FvH4_6g19690 FvH4_6g19700 FvH4_6g19700
malus_domestica	MD14G1032600.v1.1 MD14G1032900.v1.1 MD14G1033000.v1.1 MD14G1033100.v1.1
prunus_persica	Prupe.7G099900_v2.0.a1 Prupe.7G100000_v2.0.a1 Prupe.7G100000_v2.0.a1
pyrus_communis	pycom14g02930 pycom14g02940
rosa_chinensis	RchiOBHm_Chr3g0475011 RchiOBHm_Chr3g0475021 RchiOBHm_Chr3g0475031
rosa_laevigata	RLG00000023869 RLG00000023870 RLG00000023871
rosa_multiflora	Rmu_sc0003093.1_g000010 Rmu_sc0003093.1_g000011 Rmu_sc0003093.1_g000012 Rmu_sc0003093.1_g000017
rosa_roxburghii	Rroxscaffold_6G00406610 Rroxscaffold_6G00406620 Rroxscaffold_6G00406630
rosa_rugosa	Rorug03G0146000 Rorug03G0146100 Rorug03G0146200 Rorug03G0146300 Rorug03G0146300 Rorug03G0146300
rosa_samantha	Rh3AG195700 Rh3AG195900 Rh3AG196000 Rh3BG225900 Rh3BG226100 Rh3BG226200 Rh3CG220700 Rh3CG221000 Rh3CG221200 Rh3DG221200 Rh3DG221300 Rh3DG221400
rosa_wichuraiana	Rw3G017820 Rw3G017830 Rw3G017840 Rw3G017860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	322
AclWI	GGATC	2 cut(s)	175, 188
AcsI	RAATTY	1 cut(s)	6
AfaI	GTAC	2 cut(s)	191, 267
AfiI	CCNNNNNNNGG	5 cut(s)	193, 199, 200, 316, 322
AgsI	TTSAA	3 cut(s)	172, 422, 442
AjnI	CCWGG	2 cut(s)	207, 315
AluBI	AGCT	7 cut(s)	44, 100, 121, 199, 244, 293, 457
AluI	AGCT	7 cut(s)	44, 100, 121, 199, 244, 293, 457
Alw21I	GWGCWC	2 cut(s)	216, 246
AlwI	GGATC	2 cut(s)	175, 188
AlwNI	CAGNNNCTG	1 cut(s)	47
Ama87I	CYCGRG	2 cut(s)	114, 143
AoxI	GGCC	1 cut(s)	273
ApeKI	GCWGC	5 cut(s)	44, 47, 50, 121, 407
ApoI	RAATTY	1 cut(s)	6
AspS9I	GGNCC	2 cut(s)	92, 237
AsuC2I	CCSGG	1 cut(s)	194
AsuHPI	GGTGA	1 cut(s)	13
AsuII	TTCGAA	1 cut(s)	153
AvaI	CYCGRG	2 cut(s)	114, 143
AvaII	GGWCC	2 cut(s)	92, 237
BamHI	GGATCC	1 cut(s)	180
BanII	GRGCYC	2 cut(s)	144, 246
Bbv12I	GWGCWC	2 cut(s)	216, 246
BbvI	GCAGC	5 cut(s)	31, 34, 37, 108, 419
BccI	CCATC	2 cut(s)	83, 130
BciT130I	CCWGG	2 cut(s)	209, 317
BclI	TGATCA	1 cut(s)	64
BcnI	CCSGG	1 cut(s)	194
BfaI	CTAG	1 cut(s)	101
BisI	GCNGC	5 cut(s)	45, 48, 51, 122, 408
BlsI	GCNGC	5 cut(s)	46, 49, 52, 123, 409
Bme1390I	CCNGG	3 cut(s)	194, 209, 317
Bme18I	GGWCC	2 cut(s)	92, 237
BmeT110I	CYCGRG	2 cut(s)	114, 143
BmgT120I	GGNCC	2 cut(s)	92, 237
BmiI	GGNNCC	2 cut(s)	141, 182
BmrFI	CCNGG	3 cut(s)	194, 209, 317
BmsI	GCATC	2 cut(s)	292, 351
BplI	GAGNNNNNCTC	2 cut(s)	233, 265
BpmI	CTGGAG	1 cut(s)	299
Bpu14I	TTCGAA	1 cut(s)	153
BpuMI	CCSGG	1 cut(s)	194
BsaWI	WCCGGW	1 cut(s)	83
BsaXI	ACNNNNNCTCC	4 cut(s)	131, 161, 249, 279
Bsc4I	CCNNNNNNNGG	5 cut(s)	193, 199, 200, 316, 322
Bse1I	ACTGG	1 cut(s)	187
Bse3DI	GCAATG	1 cut(s)	399
BseBI	CCWGG	2 cut(s)	209, 317
BseGI	GGATG	2 cut(s)	165, 307
BseLI	CCNNNNNNNGG	5 cut(s)	193, 199, 200, 316, 322
BseMI	GCAATG	1 cut(s)	399
BseNI	ACTGG	1 cut(s)	187
BseRI	GAGGAG	1 cut(s)	190
BseXI	GCAGC	5 cut(s)	31, 34, 37, 108, 419
BseYI	CCCAGC	2 cut(s)	199, 253
BshFI	GGCC	1 cut(s)	275
BsiHKAI	GWGCWC	2 cut(s)	216, 246
BsiHKCI	CYCGRG	2 cut(s)	114, 143
BsiSI	CCGG	2 cut(s)	84, 194
BslI	CCNNNNNNNGG	5 cut(s)	193, 199, 200, 316, 322
BsmI	GAATGC	1 cut(s)	70
BsnI	GGCC	1 cut(s)	275
BsoBI	CYCGRG	2 cut(s)	114, 143
Bsp119I	TTCGAA	1 cut(s)	153
Bsp1286I	GDGCHC	3 cut(s)	144, 216, 246
Bsp143I	GATC	2 cut(s)	64, 180
BspANI	GGCC	1 cut(s)	275
BspHI	TCATGA	1 cut(s)	376
BspLI	GGNNCC	2 cut(s)	141, 182
BspPI	GGATC	2 cut(s)	175, 188
BspT104I	TTCGAA	1 cut(s)	153
BsrDI	GCAATG	1 cut(s)	399
BsrI	ACTGG	1 cut(s)	187
BssMI	GATC	2 cut(s)	64, 180
Bst2UI	CCWGG	2 cut(s)	209, 317
Bst4CI	ACNGT	2 cut(s)	36, 60
Bst6I	CTCTTC	1 cut(s)	167
BstBI	TTCGAA	1 cut(s)	153
BstC8I	GCNNGC	2 cut(s)	126, 366
BstDEI	CTNAG	1 cut(s)	287
BstF5I	GGATG	2 cut(s)	165, 307
BstKTI	GATC	2 cut(s)	67, 183
BstMBI	GATC	2 cut(s)	64, 180
BstMWI	GCNNNNNNNGC	5 cut(s)	50, 106, 250, 281, 290
BstNI	CCWGG	2 cut(s)	209, 317
BstNSI	RCATGY	1 cut(s)	368
BstSCI	CCNGG	3 cut(s)	192, 207, 315
BstV1I	GCAGC	5 cut(s)	31, 34, 37, 108, 419
BstX2I	RGATCY	1 cut(s)	180
BstYI	RGATCY	1 cut(s)	180
BsuRI	GGCC	1 cut(s)	275
BtgZI	GCGATG	1 cut(s)	149
BtsCI	GGATG	2 cut(s)	165, 307
BtsIMutI	CAGTG	1 cut(s)	56
Cac8I	GCNNGC	2 cut(s)	126, 366
CaiI	CAGNNNCTG	1 cut(s)	47
CciI	TCATGA	1 cut(s)	376
Cfr13I	GGNCC	2 cut(s)	92, 237
Csp6I	GTAC	2 cut(s)	190, 266
CviAII	CATG	4 cut(s)	298, 365, 377, 386
CviQI	GTAC	2 cut(s)	190, 266
DdeI	CTNAG	1 cut(s)	287
DpnI	GATC	2 cut(s)	66, 182
DpnII	GATC	2 cut(s)	64, 180
Eam1104I	CTCTTC	1 cut(s)	167
EarI	CTCTTC	1 cut(s)	167
Ecl136II	GAGCTC	1 cut(s)	244
Eco24I	GRGCYC	2 cut(s)	144, 246
Eco47I	GGWCC	2 cut(s)	92, 237
Eco53kI	GAGCTC	1 cut(s)	244
Eco88I	CYCGRG	2 cut(s)	114, 143
EcoICRI	GAGCTC	1 cut(s)	244
EcoRII	CCWGG	2 cut(s)	207, 315
EcoT22I	ATGCAT	1 cut(s)	366
EcoT38I	GRGCYC	2 cut(s)	144, 246
FaeI	CATG	4 cut(s)	301, 368, 380, 389
FaiI	YATR	5 cut(s)	299, 366, 378, 387, 462
FatI	CATG	4 cut(s)	297, 364, 376, 385
FbaI	TGATCA	1 cut(s)	64
Fnu4HI	GCNGC	5 cut(s)	45, 48, 51, 122, 408
FokI	GGATG	2 cut(s)	172, 314
FriOI	GRGCYC	2 cut(s)	144, 246
Fsp4HI	GCNGC	5 cut(s)	45, 48, 51, 122, 408
FspBI	CTAG	1 cut(s)	101
GluI	GCNGC	5 cut(s)	45, 48, 51, 122, 408
GsaI	CCCAGC	2 cut(s)	203, 257
GsuI	CTGGAG	1 cut(s)	299
HaeIII	GGCC	1 cut(s)	275
HapII	CCGG	2 cut(s)	84, 194
Hin1II	CATG	4 cut(s)	301, 368, 380, 389
HinfI	GANTC	5 cut(s)	104, 112, 204, 248, 380
HpaII	CCGG	2 cut(s)	84, 194
HphI	GGTGA	1 cut(s)	13
Hpy166II	GTNNAC	2 cut(s)	227, 448
Hpy188I	TCNGA	1 cut(s)	404
Hpy188III	TCNNGA	3 cut(s)	116, 235, 377
Hpy8I	GTNNAC	2 cut(s)	227, 448
HpyAV	CCTTC	2 cut(s)	286, 436
HpyCH4III	ACNGT	2 cut(s)	36, 60
HpyCH4V	TGCA	1 cut(s)	364
HpyF10VI	GCNNNNNNNGC	5 cut(s)	50, 106, 250, 281, 290
HpyF3I	CTNAG	1 cut(s)	287
Hsp92II	CATG	4 cut(s)	301, 368, 380, 389
Ksp22I	TGATCA	1 cut(s)	64
Kzo9I	GATC	2 cut(s)	64, 180
LmnI	GCTCC	4 cut(s)	139, 196, 211, 404
Lsp1109I	GCAGC	5 cut(s)	31, 34, 37, 108, 419
LweI	GCATC	2 cut(s)	292, 351
MaeI	CTAG	1 cut(s)	101
MaeIII	GTNAC	1 cut(s)	30
MalI	GATC	2 cut(s)	66, 182
MboI	GATC	2 cut(s)	64, 180
MboII	GAAGA	2 cut(s)	10, 184
MflI	RGATCY	1 cut(s)	180
MhlI	GDGCHC	3 cut(s)	144, 216, 246
MluCI	AATT	2 cut(s)	6, 320
MlyI	GAGTC	3 cut(s)	106, 113, 242
MnlI	CCTC	6 cut(s)	34, 158, 168, 171, 317, 321
Mph1103I	ATGCAT	1 cut(s)	366
MseI	TTAA	2 cut(s)	351, 472
MslI	CAYNNNNRTG	2 cut(s)	75, 369
MspA1I	CMGCKG	1 cut(s)	44
MspI	CCGG	2 cut(s)	84, 194
MspR9I	CCNGG	3 cut(s)	194, 209, 317
Mva1269I	GAATGC	1 cut(s)	70
MvaI	CCWGG	2 cut(s)	209, 317
MwoI	GCNNNNNNNGC	5 cut(s)	50, 106, 250, 281, 290
NciI	CCSGG	1 cut(s)	194
NdeII	GATC	2 cut(s)	64, 180
NlaIII	CATG	4 cut(s)	301, 368, 380, 389
NlaIV	GGNNCC	2 cut(s)	141, 182
NsiI	ATGCAT	1 cut(s)	366
NspI	RCATGY	1 cut(s)	368
NspV	TTCGAA	1 cut(s)	153
PaeI	GCATGC	1 cut(s)	368
PaeR7I	CTCGAG	1 cut(s)	114
PagI	TCATGA	1 cut(s)	376
PcsI	WCGNNNNNNNCGW	1 cut(s)	237
PctI	GAATGC	1 cut(s)	70
PfeI	GAWTC	2 cut(s)	204, 380
PflMI	CCANNNNNTGG	1 cut(s)	322
PfoI	TCCNGGA	2 cut(s)	207, 315
PkrI	GCNGC	5 cut(s)	46, 49, 52, 123, 409
PleI	GAGTC	3 cut(s)	106, 112, 242
PpsI	GAGTC	3 cut(s)	106, 112, 242
Psp124BI	GAGCTC	1 cut(s)	246
Psp6I	CCWGG	2 cut(s)	207, 315
PspFI	CCCAGC	2 cut(s)	199, 253
PspGI	CCWGG	2 cut(s)	207, 315
PspN4I	GGNNCC	2 cut(s)	141, 182
PspPI	GGNCC	2 cut(s)	92, 237
PstNI	CAGNNNCTG	1 cut(s)	47
PsuI	RGATCY	1 cut(s)	180
PvuII	CAGCTG	1 cut(s)	44
RsaI	GTAC	2 cut(s)	191, 267
RsaNI	GTAC	2 cut(s)	190, 266
RseI	CAYNNNNRTG	2 cut(s)	75, 369
SacI	GAGCTC	1 cut(s)	246
SaqAI	TTAA	2 cut(s)	351, 472
SatI	GCNGC	5 cut(s)	45, 48, 51, 122, 408
Sau3AI	GATC	2 cut(s)	64, 180
Sau96I	GGNCC	2 cut(s)	92, 237
SchI	GAGTC	3 cut(s)	106, 113, 242
ScrFI	CCNGG	3 cut(s)	194, 209, 317
SduI	GDGCHC	3 cut(s)	144, 216, 246
SetI	ASST	9 cut(s)	26, 46, 102, 123, 201, 246, 295, 447, 459
SfaNI	GCATC	2 cut(s)	292, 351
Sfr274I	CTCGAG	1 cut(s)	114
SfuI	TTCGAA	1 cut(s)	153
SinI	GGWCC	2 cut(s)	92, 237
SlaI	CTCGAG	1 cut(s)	114
SmiMI	CAYNNNNRTG	2 cut(s)	75, 369
SmlI	CTYRAG	1 cut(s)	114
SmoI	CTYRAG	1 cut(s)	114
SphI	GCATGC	1 cut(s)	368
Sse9I	AATT	2 cut(s)	6, 320
SspMI	CTAG	1 cut(s)	101
SstI	GAGCTC	1 cut(s)	246
StyD4I	CCNGG	3 cut(s)	192, 207, 315
TaaI	ACNGT	2 cut(s)	36, 60
TaqI	TCGA	3 cut(s)	115, 153, 246
TaqII	GACCGA	1 cut(s)	254
TasI	AATT	2 cut(s)	6, 320
TfiI	GAWTC	2 cut(s)	204, 380
Tru1I	TTAA	2 cut(s)	351, 472
Tru9I	TTAA	2 cut(s)	351, 472
TscAI	CASTG	1 cut(s)	63
TseI	GCWGC	5 cut(s)	44, 47, 50, 121, 407
TspRI	CASTG	1 cut(s)	63
Van91I	CCANNNNNTGG	1 cut(s)	322
VpaK11BI	GGWCC	2 cut(s)	92, 237
XapI	RAATTY	1 cut(s)	6
XceI	RCATGY	1 cut(s)	368
XhoI	CTCGAG	1 cut(s)	114
XspI	CTAG	1 cut(s)	101
Zsp2I	ATGCAT	1 cut(s)	366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.