Rmu_sc0003093.1_g000017

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003093.1
OG0001332
Physical Location & Seq
Reverse (-)
77804 .. 80702
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003093.1_g000017.1.cds

Sequence Viewer

Length: 399 bp
atggcgggaaggatcacagaggctctggccactcagctccaggatgccttcgtcccgcacctcttgttcaagcaggtatcatggggtctagagagcactgccggttcgcgaccggagctgagtcaactgagtcgggagttgctggactcgaccagggagcctgaattctttgattggttgagaaaagtcaagaggaaaatccacgagaaccctgagctcgcctttgaggaggacgagactagccgattcatacgatcggagctcgacttgcttgggatagagtacacatggccagtggccaagacgggggtggtggcttctatcggttctgggttgcagcattggttcactctcagggcagacatggatgctctcccaattgaggtcagtcccttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

132

Amino Acids

15.04

Weight (kDa)

4.94

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 64
AccII CGCG 1 cut(s) 109
AciI CCGC 2 cut(s) 5, 56
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 3 cut(s) 27, 290, 297
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 2 cut(s) 306, 382
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 2 cut(s) 39, 152
AluBI AGCT 4 cut(s) 37, 118, 217, 262
AluI AGCT 4 cut(s) 37, 118, 217, 262
Alw21I GWGCWC 3 cut(s) 98, 219, 264
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 1 cut(s) 20
AoxI GGCC 3 cut(s) 27, 290, 297
ApeKI GCWGC 1 cut(s) 337
ApoI RAATTY 1 cut(s) 164
BalI TGGCCA 3 cut(s) 29, 292, 299
BanII GRGCYC 2 cut(s) 219, 264
BauI CACGAG 1 cut(s) 203
Bbv12I GWGCWC 3 cut(s) 98, 219, 264
BbvI GCAGC 1 cut(s) 349
BciT130I CCWGG 2 cut(s) 41, 154
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 3 cut(s) 89, 240, 397
BfuAI ACCTGC 1 cut(s) 64
BisI GCNGC 1 cut(s) 338
BlsI GCNGC 1 cut(s) 339
Bme1390I CCNGG 2 cut(s) 41, 154
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 41, 154
BmsI GCATC 2 cut(s) 34, 358
BpmI CTGGAG 1 cut(s) 23
Bpu10I CCTNAGC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 382
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 293
BseBI CCWGG 2 cut(s) 41, 154
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 2 cut(s) 49, 373
BseLI CCNNNNNNNGG 2 cut(s) 306, 382
BseMII CTCAG 5 cut(s) 47, 110, 119, 204, 367
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 109
Bsh1285I CGRYCG 2 cut(s) 113, 257
BshFI GGCC 3 cut(s) 29, 292, 299
BsiEI CGRYCG 2 cut(s) 113, 257
BsiHKAI GWGCWC 3 cut(s) 98, 219, 264
BsiSI CCGG 2 cut(s) 102, 113
BslFI GGGAC 2 cut(s) 38, 375
BslI CCNNNNNNNGG 2 cut(s) 306, 382
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 38, 375
BsnI GGCC 3 cut(s) 29, 292, 299
Bsp1286I GDGCHC 3 cut(s) 98, 219, 264
Bsp143I GATC 2 cut(s) 12, 254
Bsp68I TCGCGA 1 cut(s) 109
BspACI CCGC 2 cut(s) 5, 56
BspANI GGCC 3 cut(s) 29, 292, 299
BspCNI CTCAG 5 cut(s) 46, 111, 120, 205, 366
BspFNI CGCG 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 64
BspPI GGATC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 12, 254
BssSI CACGAG 1 cut(s) 203
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 2 cut(s) 41, 154
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 5 cut(s) 33, 119, 128, 213, 353
BstF5I GGATG 2 cut(s) 49, 373
BstFNI CGCG 1 cut(s) 109
BstKTI GATC 2 cut(s) 15, 257
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 2 cut(s) 12, 254
BstMCI CGRYCG 2 cut(s) 113, 257
BstMWI GCNNNNNNNGC 2 cut(s) 115, 268
BstNI CCWGG 2 cut(s) 41, 154
BstSCI CCNGG 2 cut(s) 39, 152
BstUI CGCG 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 349
BsuRI GGCC 3 cut(s) 29, 292, 299
BtsCI GGATG 2 cut(s) 49, 373
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 300
BtuMI TCGCGA 1 cut(s) 109
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 101
Csp6I GTAC 1 cut(s) 283
CviAII CATG 3 cut(s) 81, 288, 364
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 5 cut(s) 33, 119, 128, 213, 353
DpnI GATC 2 cut(s) 14, 256
DpnII GATC 2 cut(s) 12, 254
EaeI YGGCCR 3 cut(s) 27, 290, 297
Ecl136II GAGCTC 2 cut(s) 217, 262
Eco24I GRGCYC 2 cut(s) 219, 264
Eco53kI GAGCTC 2 cut(s) 217, 262
EcoICRI GAGCTC 2 cut(s) 217, 262
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 39, 152
EcoT38I GRGCYC 2 cut(s) 219, 264
FaeI CATG 3 cut(s) 84, 291, 367
FaiI YATR 4 cut(s) 82, 251, 289, 365
FaqI GGGAC 2 cut(s) 38, 375
FatI CATG 3 cut(s) 80, 287, 363
FauI CCCGC 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 338
FokI GGATG 2 cut(s) 56, 380
FriOI GRGCYC 2 cut(s) 219, 264
Fsp4HI GCNGC 1 cut(s) 338
FspBI CTAG 3 cut(s) 89, 240, 397
GluI GCNGC 1 cut(s) 338
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 3 cut(s) 29, 292, 299
HapII CCGG 2 cut(s) 102, 113
Hin1II CATG 3 cut(s) 84, 291, 367
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HinfI GANTC 4 cut(s) 121, 130, 146, 246
HpaII CCGG 2 cut(s) 102, 113
Hpy166II GTNNAC 3 cut(s) 125, 285, 348
Hpy188I TCNGA 1 cut(s) 259
Hpy188III TCNNGA 4 cut(s) 89, 108, 134, 190
Hpy8I GTNNAC 3 cut(s) 125, 285, 348
HpyAV CCTTC 2 cut(s) 3, 58
HpyCH4V TGCA 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 268
HpyF3I CTNAG 5 cut(s) 33, 119, 128, 213, 353
Hsp92II CATG 3 cut(s) 84, 291, 367
Kzo9I GATC 2 cut(s) 12, 254
LmnI GCTCC 4 cut(s) 42, 115, 157, 259
Lsp1109I GCAGC 1 cut(s) 349
LweI GCATC 2 cut(s) 34, 358
MaeI CTAG 3 cut(s) 89, 240, 397
MalI GATC 2 cut(s) 14, 256
MboI GATC 2 cut(s) 12, 254
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 3 cut(s) 98, 219, 264
MlsI TGGCCA 3 cut(s) 29, 292, 299
MluCI AATT 2 cut(s) 164, 378
MluNI TGGCCA 3 cut(s) 29, 292, 299
MlyI GAGTC 3 cut(s) 130, 139, 140
MnlI CCTC 6 cut(s) 13, 71, 186, 220, 223, 376
Mox20I TGGCCA 3 cut(s) 29, 292, 299
MscI TGGCCA 3 cut(s) 29, 292, 299
Msp20I TGGCCA 3 cut(s) 29, 292, 299
MspI CCGG 2 cut(s) 102, 113
MspR9I CCNGG 2 cut(s) 41, 154
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 2 cut(s) 41, 154
MvnI CGCG 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 115, 268
NdeII GATC 2 cut(s) 12, 254
NlaIII CATG 3 cut(s) 84, 291, 367
NlaIV GGNNCC 1 cut(s) 159
NruI TCGCGA 1 cut(s) 109
PfeI GAWTC 1 cut(s) 246
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 1 cut(s) 339
Ple19I CGATCG 1 cut(s) 257
PleI GAGTC 3 cut(s) 129, 138, 140
PpsI GAGTC 3 cut(s) 129, 138, 140
Psp124BI GAGCTC 2 cut(s) 219, 264
Psp6I CCWGG 2 cut(s) 39, 152
PspGI CCWGG 2 cut(s) 39, 152
PspN4I GGNNCC 1 cut(s) 159
PvuI CGATCG 1 cut(s) 257
RruI TCGCGA 1 cut(s) 109
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SacI GAGCTC 2 cut(s) 219, 264
SatI GCNGC 1 cut(s) 338
Sau3AI GATC 2 cut(s) 12, 254
SchI GAGTC 3 cut(s) 130, 139, 140
ScrFI CCNGG 2 cut(s) 41, 154
SduI GDGCHC 3 cut(s) 98, 219, 264
SetI ASST 7 cut(s) 39, 63, 78, 120, 219, 264, 387
SfaNI GCATC 2 cut(s) 34, 358
Sse9I AATT 2 cut(s) 164, 378
SsiI CCGC 2 cut(s) 5, 56
SspMI CTAG 3 cut(s) 89, 240, 397
SstI GAGCTC 2 cut(s) 219, 264
StyD4I CCNGG 2 cut(s) 39, 152
TaqI TCGA 2 cut(s) 149, 264
TasI AATT 2 cut(s) 164, 378
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 1 cut(s) 246
TscAI CASTG 2 cut(s) 103, 300
TseI GCWGC 1 cut(s) 337
TspDTI ATGAA 1 cut(s) 238
TspRI CASTG 2 cut(s) 103, 300
XapI RAATTY 1 cut(s) 164
XbaI TCTAGA 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XspI CTAG 3 cut(s) 89, 240, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.