Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0001332

Physical Location & Seq

Genomic Coordinates Forward (+)

29146227 .. 29150878

Gene Structure 4652 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00406630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1347 bp

ATGGTTTCCACTTTCACTTTCGGCTTATTACTAGCTGCAACAGTGTGGTTTTACCAATCTCAGGCTTCAGAGGATGCTGATTGTGGATCAGAGCACGAGTTCCTGACTTTGGAGTTGAGGAAATCGGTGAGAGAGCCTGAGTTCTTTAAATGGATGATAGGGTTGAGAAGAAGAATTCATCAGTATCCAGAGTTGGGATTTCAAGAGCATAAGACGAGTGAACTCATCAGGTCCGAGCTTGACTCCCTTGGAGTTAAGTACAAGTGGCCGGTGGCCAAGACTGGAGTGGTGGCTTCAATTGGGAAAGGGTCTAAACCAGTCTTTGCTCTTAGAGCTGACATGGATGCTCTCCCTCTCCAGGAACTAGTGGAGTGGGAGTACAAGAGCAAGATTGATGGAAAGATGCATGCTTGCGGTCACGATTCACATGTCGCAATGCTACTAGGAGCAGCCAAATTGCTTCAAGAAAAAAGAGATATATTGAAGCTAGTTTTCCAGCCTGGAGAAGAGGGTTATGCTGGCGCTTACCATATGTTACAAGATGGTGTTTTAGATGATATTGATGGCATCTTTGCCCTGCATGTTTATCCACATATACCAACAGGTACTATAGCTTCGAGGCCTGGTCAAATAGTTGGTGGTGTAGGGCTCTTCAAAGCTACAATACAAGGAATAGGAGGTCATGGGGCAGCGCCACATAAGACCAGGGACCCTATTGTTGCAGCAGCCTTCGCAACACTTGCTCTGCAACAGATTGTGTCTCGAGAGAAGAATCCTCTTGAACCTGGAGTGGTAACAGTAGGGTACATACAGGGCGGTCAAGCAGATAATGTGATCCCAGAGAGTGTGAAGCTTGGAGGAACCTACAGGAGCTTGACTTCAGAGGGCCTCTACTATATCCAAGAAAGGATCAGAGAGGTCATGGAGCAGCAAGCAGCTGTGCATCGTTGTTCTGCTGTGGTTGACTTCCTGGAGGAAATACCCCTGCCTCATCCACCAATGGCTAATGATCAATCGCTCTATGAACATGTGAAGAATGTTGGTCAGGTCCTTCTTGGGAAACCAAATGTGCGGCTTCTGCCACTGACCATGGTTGCAGAGGATTTCAGCTACTTTGCTGAGAAGACTGCTGCTGCACTTTTTGTTATTGGTACAGGGAATGAGACACTAAAATCAGATAAAACAGCACACTCACCCTACTTTTTCATTGACGAGGAGGCTCTTCCATTGGGAGCAGCACTTCATGCTGCAATTGCAATCTCATACTTGGATGACCATGACTCAGTAGAGCATGTCTCACTCTTCAATTCTGGAGATAAACAGTGCTTAGCATATGAAGTGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010210 GO:0010211 GO:0010817 GO:0012505 GO:0016787 GO:0016810 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

448

Amino Acids

49.24

Weight (kDa)

5.57

Isoelectric Point (pI)

39.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	109 - 419	2.7e-38	Peptidase family M20/M25/M40
M20_dimer	PF07687	217 - 313	1e-11	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02875 AT3G02875 AT3G02875
fragaria_vesca	FvH4_6g19670 FvH4_6g19670 FvH4_6g19670 FvH4_6g19670 FvH4_6g19690 FvH4_6g19700 FvH4_6g19700
malus_domestica	MD14G1032600.v1.1 MD14G1032900.v1.1 MD14G1033000.v1.1 MD14G1033100.v1.1
prunus_persica	Prupe.7G099900_v2.0.a1 Prupe.7G100000_v2.0.a1 Prupe.7G100000_v2.0.a1
pyrus_communis	pycom14g02930 pycom14g02940
rosa_chinensis	RchiOBHm_Chr3g0475011 RchiOBHm_Chr3g0475021 RchiOBHm_Chr3g0475031
rosa_laevigata	RLG00000023869 RLG00000023870 RLG00000023871
rosa_multiflora	Rmu_sc0003093.1_g000010 Rmu_sc0003093.1_g000011 Rmu_sc0003093.1_g000012 Rmu_sc0003093.1_g000017
rosa_roxburghii	Rroxscaffold_6G00406610 Rroxscaffold_6G00406620 Rroxscaffold_6G00406630
rosa_rugosa	Rorug03G0146000 Rorug03G0146100 Rorug03G0146200 Rorug03G0146300 Rorug03G0146300 Rorug03G0146300
rosa_samantha	Rh3AG195700 Rh3AG195900 Rh3AG196000 Rh3BG225900 Rh3BG226100 Rh3BG226200 Rh3CG220700 Rh3CG221000 Rh3CG221200 Rh3DG221200 Rh3DG221300 Rh3DG221400
rosa_wichuraiana	Rw3G017820 Rw3G017830 Rw3G017840 Rw3G017860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	414, 816, 1072
AclWI	GGATC	3 cut(s)	94, 829, 917
AcoI	YGGCCR	2 cut(s)	266, 273
AcsI	RAATTY	1 cut(s)	174
AcuI	CTGAAG	2 cut(s)	51, 864
AfaI	GTAC	5 cut(s)	260, 380, 607, 806, 1153
AfiI	CCNNNNNNNGG	4 cut(s)	61, 109, 194, 358
AflIII	ACRYGT	2 cut(s)	427, 1027
AgsI	TTSAA	7 cut(s)	203, 297, 464, 484, 655, 782, 1306
AhlI	ACTAGT	1 cut(s)	364
AjnI	CCWGG	6 cut(s)	357, 499, 622, 704, 784, 969
Alw21I	GWGCWC	1 cut(s)	96
Alw26I	GTCTC	3 cut(s)	765, 1157, 1300
AlwI	GGATC	3 cut(s)	94, 829, 917
Ama87I	CYCGRG	1 cut(s)	762
AoxI	GGCC	4 cut(s)	266, 273, 620, 886
ApoI	RAATTY	1 cut(s)	174
AspLEI	GCGC	2 cut(s)	524, 694
AspS9I	GGNCC	4 cut(s)	231, 709, 886, 1048
AsuHPI	GGTGA	2 cut(s)	139, 1185
AvaI	CYCGRG	1 cut(s)	762
AvaII	GGWCC	3 cut(s)	231, 709, 1048
BalI	TGGCCA	1 cut(s)	275
BanII	GRGCYC	1 cut(s)	651
BarI	GAAGNNNNNNTAC	2 cut(s)	598, 630
BauI	CACGAG	1 cut(s)	95
BbsI	GAAGAC	1 cut(s)	1130
Bbv12I	GWGCWC	1 cut(s)	96
BccI	CCATC	3 cut(s)	389, 536, 557
BciT130I	CCWGG	6 cut(s)	359, 501, 624, 706, 786, 971
BciVI	GTATCC	1 cut(s)	195
BclI	TGATCA	1 cut(s)	1009
BcoDI	GTCTC	3 cut(s)	765, 1157, 1300
BcuI	ACTAGT	1 cut(s)	364
BfaI	CTAG	4 cut(s)	32, 365, 443, 488
BfmI	CTRYAG	2 cut(s)	609, 865
BfoI	RGCGCY	2 cut(s)	525, 695
BfuI	GTATCC	1 cut(s)	195
BlpI	GCTNAGC	1 cut(s)	1327
Bme1390I	CCNGG	6 cut(s)	359, 501, 624, 706, 786, 971
Bme18I	GGWCC	3 cut(s)	231, 709, 1048
BmeT110I	CYCGRG	1 cut(s)	762
BmgT120I	GGNCC	4 cut(s)	231, 709, 886, 1048
BmiI	GGNNCC	3 cut(s)	710, 711, 862
BmrFI	CCNGG	6 cut(s)	359, 501, 624, 706, 786, 971
BmsI	GCATC	5 cut(s)	64, 334, 393, 576, 952
BpiI	GAAGAC	1 cut(s)	1130
BplI	GAGNNNNNCTC	4 cut(s)	227, 259, 1280, 1312
BpmI	CTGGAG	6 cut(s)	303, 341, 522, 807, 992, 1332
Bpu1102I	GCTNAGC	1 cut(s)	1327
BsaJI	CCNNGG	3 cut(s)	247, 705, 1089
BsaXI	ACNNNNNCTCC	2 cut(s)	362, 392
Bsc4I	CCNNNNNNNGG	4 cut(s)	61, 109, 194, 358
Bse118I	RCCGGY	1 cut(s)	268
Bse1I	ACTGG	2 cut(s)	286, 317
Bse3DI	GCAATG	1 cut(s)	441
BseBI	CCWGG	6 cut(s)	359, 501, 624, 706, 786, 971
BseDI	CCNNGG	3 cut(s)	247, 705, 1089
BseGI	GGATG	5 cut(s)	79, 159, 349, 991, 1276
BseLI	CCNNNNNNNGG	4 cut(s)	61, 109, 194, 358
BseMI	GCAATG	1 cut(s)	441
BseMII	CTCAG	4 cut(s)	74, 129, 1110, 1296
BseNI	ACTGG	2 cut(s)	286, 317
BseRI	GAGGAG	1 cut(s)	1229
BsgI	GTGCAG	1 cut(s)	1119
BshFI	GGCC	4 cut(s)	268, 275, 622, 888
BsiHKAI	GWGCWC	1 cut(s)	96
BsiHKCI	CYCGRG	1 cut(s)	762
BsiSI	CCGG	1 cut(s)	269
BslFI	GGGAC	1 cut(s)	722
BslI	CCNNNNNNNGG	4 cut(s)	61, 109, 194, 358
BsmAI	GTCTC	3 cut(s)	765, 1157, 1300
BsmFI	GGGAC	1 cut(s)	722
BsnI	GGCC	4 cut(s)	268, 275, 622, 888
BsoBI	CYCGRG	1 cut(s)	762
Bsp1286I	GDGCHC	2 cut(s)	96, 651
Bsp143I	GATC	4 cut(s)	86, 834, 909, 1009
Bsp1720I	GCTNAGC	1 cut(s)	1327
Bsp19I	CCATGG	1 cut(s)	1089
BspACI	CCGC	3 cut(s)	414, 816, 1072
BspANI	GGCC	4 cut(s)	268, 275, 622, 888
BspCNI	CTCAG	4 cut(s)	73, 130, 1111, 1295
BspLI	GGNNCC	3 cut(s)	710, 711, 862
BspPI	GGATC	3 cut(s)	94, 829, 917
BspQI	GCTCTTC	2 cut(s)	656, 1227
BsrDI	GCAATG	1 cut(s)	441
BsrFI	RCCGGY	1 cut(s)	268
BsrI	ACTGG	2 cut(s)	286, 317
BssAI	RCCGGY	1 cut(s)	268
BssECI	CCNNGG	3 cut(s)	247, 705, 1089
BssMI	GATC	4 cut(s)	86, 834, 909, 1009
BssSI	CACGAG	1 cut(s)	95
BssT1I	CCWWGG	2 cut(s)	247, 1089
Bst2BI	CACGAG	1 cut(s)	95
Bst2UI	CCWGG	6 cut(s)	359, 501, 624, 706, 786, 971
Bst4CI	ACNGT	3 cut(s)	43, 799, 1323
Bst6I	CTCTTC	4 cut(s)	501, 656, 1227, 1307
BstAPI	GCANNNNNTGC	2 cut(s)	740, 1244
BstC8I	GCNNGC	4 cut(s)	408, 412, 520, 933
BstDEI	CTNAG	6 cut(s)	60, 138, 329, 1119, 1282, 1327
BstDSI	CCRYGG	1 cut(s)	1089
BstF5I	GGATG	5 cut(s)	79, 159, 349, 991, 1276
BstH2I	RGCGCY	2 cut(s)	525, 695
BstHHI	GCGC	2 cut(s)	524, 694
BstKTI	GATC	4 cut(s)	89, 837, 912, 1012
BstMAI	GTCTC	3 cut(s)	765, 1157, 1300
BstMBI	GATC	4 cut(s)	86, 834, 909, 1009
BstMWI	GCNNNNNNNGC	6 cut(s)	332, 731, 740, 1078, 1244, 1253
BstNI	CCWGG	6 cut(s)	359, 501, 624, 706, 786, 971
BstNSI	RCATGY	5 cut(s)	410, 431, 584, 1031, 1295
BstSCI	CCNGG	6 cut(s)	357, 499, 622, 704, 784, 969
BstSFI	CTRYAG	2 cut(s)	609, 865
BstV2I	GAAGAC	1 cut(s)	1130
BsuI	GTATCC	1 cut(s)	195
BsuRI	GGCC	4 cut(s)	268, 275, 622, 888
BtgI	CCRYGG	1 cut(s)	1089
BtsCI	GGATG	5 cut(s)	79, 159, 349, 991, 1276
BtsIMutI	CAGTG	3 cut(s)	48, 1082, 1328
Cac8I	GCNNGC	4 cut(s)	408, 412, 520, 933
CfoI	GCGC	2 cut(s)	524, 694
Cfr10I	RCCGGY	1 cut(s)	268
Cfr13I	GGNCC	4 cut(s)	231, 709, 886, 1048
Csp6I	GTAC	5 cut(s)	259, 379, 606, 805, 1152
CviQI	GTAC	5 cut(s)	259, 379, 606, 805, 1152
DdeI	CTNAG	6 cut(s)	60, 138, 329, 1119, 1282, 1327
DpnI	GATC	4 cut(s)	88, 836, 911, 1011
DpnII	GATC	4 cut(s)	86, 834, 909, 1009
DraI	TTTAAA	1 cut(s)	148
EaeI	YGGCCR	2 cut(s)	266, 273
Eam1104I	CTCTTC	4 cut(s)	501, 656, 1227, 1307
EarI	CTCTTC	4 cut(s)	501, 656, 1227, 1307
Eco130I	CCWWGG	2 cut(s)	247, 1089
Eco147I	AGGCCT	1 cut(s)	622
Eco24I	GRGCYC	1 cut(s)	651
Eco47I	GGWCC	3 cut(s)	231, 709, 1048
Eco57I	CTGAAG	2 cut(s)	51, 864
Eco88I	CYCGRG	1 cut(s)	762
EcoO109I	RGGNCCY	3 cut(s)	709, 886, 1048
EcoRI	GAATTC	1 cut(s)	174
EcoRII	CCWGG	6 cut(s)	357, 499, 622, 704, 784, 969
EcoT14I	CCWWGG	2 cut(s)	247, 1089
EcoT22I	ATGCAT	1 cut(s)	408
EcoT38I	GRGCYC	1 cut(s)	651
ErhI	CCWWGG	2 cut(s)	247, 1089
FaqI	GGGAC	1 cut(s)	722
FauNDI	CATATG	2 cut(s)	531, 1333
FbaI	TGATCA	1 cut(s)	1009
FokI	GGATG	5 cut(s)	86, 166, 356, 978, 1283
FriOI	GRGCYC	1 cut(s)	651
FspBI	CTAG	4 cut(s)	32, 365, 443, 488
GlaI	GCGC	2 cut(s)	523, 693
GsuI	CTGGAG	6 cut(s)	303, 341, 522, 807, 992, 1332
HaeII	RGCGCY	2 cut(s)	525, 695
HaeIII	GGCC	4 cut(s)	268, 275, 622, 888
HapII	CCGG	1 cut(s)	269
HhaI	GCGC	2 cut(s)	524, 694
Hin6I	GCGC	2 cut(s)	522, 692
HinP1I	GCGC	2 cut(s)	522, 692
HincII	GTYRAC	1 cut(s)	964
HindII	GTYRAC	1 cut(s)	964
HindIII	AAGCTT	1 cut(s)	851
HinfI	GANTC	4 cut(s)	242, 422, 772, 1280
HpaII	CCGG	1 cut(s)	269
HphI	GGTGA	2 cut(s)	139, 1185
Hpy166II	GTNNAC	2 cut(s)	221, 964
Hpy188I	TCNGA	6 cut(s)	70, 91, 235, 883, 914, 1177
Hpy188III	TCNNGA	9 cut(s)	103, 188, 203, 419, 464, 762, 764, 779, 1311
Hpy8I	GTNNAC	2 cut(s)	221, 964
HpyAV	CCTTC	2 cut(s)	739, 1061
HpyCH4III	ACNGT	3 cut(s)	43, 799, 1323
HpyF10VI	GCNNNNNNNGC	6 cut(s)	332, 731, 740, 1078, 1244, 1253
HpyF3I	CTNAG	6 cut(s)	60, 138, 329, 1119, 1282, 1327
HspAI	GCGC	2 cut(s)	522, 692
KflI	GGGWCCC	1 cut(s)	709
Ksp22I	TGATCA	1 cut(s)	1009
Kzo9I	GATC	4 cut(s)	86, 834, 909, 1009
LguI	GCTCTTC	2 cut(s)	656, 1227
LmnI	GCTCC	4 cut(s)	446, 870, 925, 1232
LweI	GCATC	5 cut(s)	64, 334, 393, 576, 952
MaeI	CTAG	4 cut(s)	32, 365, 443, 488
MaeIII	GTNAC	3 cut(s)	416, 534, 793
MalI	GATC	4 cut(s)	88, 836, 911, 1011
MboI	GATC	4 cut(s)	86, 834, 909, 1009
MboII	GAAGA	9 cut(s)	180, 183, 518, 643, 781, 1045, 1135, 1214, 1294
MfeI	CAATTG	2 cut(s)	297, 1251
MhlI	GDGCHC	2 cut(s)	96, 651
MlsI	TGGCCA	1 cut(s)	275
MluCI	AATT	5 cut(s)	174, 297, 455, 1251, 1306
MluNI	TGGCCA	1 cut(s)	275
MlyI	GAGTC	2 cut(s)	236, 1274
Mox20I	TGGCCA	1 cut(s)	275
Mph1103I	ATGCAT	1 cut(s)	408
MscI	TGGCCA	1 cut(s)	275
MseI	TTAA	2 cut(s)	147, 255
Msp20I	TGGCCA	1 cut(s)	275
MspA1I	CMGCKG	1 cut(s)	938
MspI	CCGG	1 cut(s)	269
MspR9I	CCNGG	6 cut(s)	359, 501, 624, 706, 786, 971
MunI	CAATTG	2 cut(s)	297, 1251
MvaI	CCWGG	6 cut(s)	359, 501, 624, 706, 786, 971
MwoI	GCNNNNNNNGC	6 cut(s)	332, 731, 740, 1078, 1244, 1253
NcoI	CCATGG	1 cut(s)	1089
NdeI	CATATG	2 cut(s)	531, 1333
NdeII	GATC	4 cut(s)	86, 834, 909, 1009
NlaIV	GGNNCC	3 cut(s)	710, 711, 862
NmuCI	GTSAC	1 cut(s)	416
NsiI	ATGCAT	1 cut(s)	408
NspI	RCATGY	5 cut(s)	410, 431, 584, 1031, 1295
PaeI	GCATGC	1 cut(s)	410
PaeR7I	CTCGAG	1 cut(s)	762
PceI	AGGCCT	1 cut(s)	622
PciI	ACATGT	2 cut(s)	427, 1027
PciSI	GCTCTTC	2 cut(s)	656, 1227
PfeI	GAWTC	2 cut(s)	422, 772
PfoI	TCCNGGA	2 cut(s)	357, 969
PleI	GAGTC	2 cut(s)	236, 1274
PpsI	GAGTC	2 cut(s)	236, 1274
PpuMI	RGGWCCY	2 cut(s)	709, 1048
PscI	ACATGT	2 cut(s)	427, 1027
Psp5II	RGGWCCY	2 cut(s)	709, 1048
Psp6I	CCWGG	6 cut(s)	357, 499, 622, 704, 784, 969
PspGI	CCWGG	6 cut(s)	357, 499, 622, 704, 784, 969
PspN4I	GGNNCC	3 cut(s)	710, 711, 862
PspPI	GGNCC	4 cut(s)	231, 709, 886, 1048
PspPPI	RGGWCCY	2 cut(s)	709, 1048
PvuII	CAGCTG	1 cut(s)	938
RsaI	GTAC	5 cut(s)	260, 380, 607, 806, 1153
RsaNI	GTAC	5 cut(s)	259, 379, 606, 805, 1152
SapI	GCTCTTC	2 cut(s)	656, 1227
SaqAI	TTAA	2 cut(s)	147, 255
Sau3AI	GATC	4 cut(s)	86, 834, 909, 1009
Sau96I	GGNCC	4 cut(s)	231, 709, 886, 1048
SchI	GAGTC	2 cut(s)	236, 1274
ScrFI	CCNGG	6 cut(s)	359, 501, 624, 706, 786, 971
SduI	GDGCHC	2 cut(s)	96, 651
SfaNI	GCATC	5 cut(s)	64, 334, 393, 576, 952
SfcI	CTRYAG	2 cut(s)	609, 865
Sfr274I	CTCGAG	1 cut(s)	762
SinI	GGWCC	3 cut(s)	231, 709, 1048
SlaI	CTCGAG	1 cut(s)	762
SmlI	CTYRAG	1 cut(s)	762
SmoI	CTYRAG	1 cut(s)	762
SpeI	ACTAGT	1 cut(s)	364
SphI	GCATGC	1 cut(s)	410
Sse9I	AATT	5 cut(s)	174, 297, 455, 1251, 1306
SseBI	AGGCCT	1 cut(s)	622
SsiI	CCGC	3 cut(s)	414, 816, 1072
SspMI	CTAG	4 cut(s)	32, 365, 443, 488
StuI	AGGCCT	1 cut(s)	622
StyD4I	CCNGG	6 cut(s)	357, 499, 622, 704, 784, 969
StyI	CCWWGG	2 cut(s)	247, 1089
TaaI	ACNGT	3 cut(s)	43, 799, 1323
TaqI	TCGA	2 cut(s)	617, 763
TasI	AATT	5 cut(s)	174, 297, 455, 1251, 1306
TatI	WGTACW	2 cut(s)	258, 378
TauI	GCSGC	1 cut(s)	1075
TfiI	GAWTC	2 cut(s)	422, 772
Tru1I	TTAA	2 cut(s)	147, 255
Tru9I	TTAA	2 cut(s)	147, 255
TscAI	CASTG	3 cut(s)	48, 1089, 1328
TseFI	GTSAC	1 cut(s)	416
Tsp45I	GTSAC	1 cut(s)	416
TspDTI	ATGAA	4 cut(s)	167, 1038, 1195, 1232
TspRI	CASTG	3 cut(s)	48, 1089, 1328
VpaK11BI	GGWCC	3 cut(s)	231, 709, 1048
XapI	RAATTY	1 cut(s)	174
XceI	RCATGY	5 cut(s)	410, 431, 584, 1031, 1295
XcmI	CCANNNNNNNNNTGG	1 cut(s)	283
XhoI	CTCGAG	1 cut(s)	762
XspI	CTAG	4 cut(s)	32, 365, 443, 488
Zsp2I	ATGCAT	1 cut(s)	408

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.