Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001332

Physical Location & Seq

Genomic Coordinates Reverse (-)

2947357 .. 2950167

Gene Structure 2811 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1033100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGCTTTTGTGCTTATTAATGCAAACTATTTTGGGCCAATCATGGGCTTCGGAAACCAGTGAGACAGAGCTCGAGTTGCTGACTCGGGAGCTATTGGCATCGGCGAAGGAGGCCGAGTTCTTTGAGTGGATGAGGGGAGTGAGGAGGAGAATCCACCAGTACCCAGAGATTGGGTTCGAGGAGCACAGGACAAGTCAGCTCATCAGGACGGAGCTCGACACGCTAGGAATTGAGTACACGTGGCCTGTTGCCAAGACTGGTGTGGTGGCTTCCATTGGTTCTGGCTCCAAACCAGTGTTTGCTCTCAGGGCTGACATGGACGCCCTCCCTCTTCAGGAACAAGTAGAGTGGGAGTTCAAGAGCAAGATAGAGGGCAAAATGCACGCATGTGGTCACGATTCTCATGTGGCAATGTTGCTTGGCGCAGTCAAGTTACTCCAAAGTAAAAGAGACATTCTAAAGAAGAGGTTTATGCCGGAGCTTACCATATGTTACAACATGGCATTCTTAACGACATTGATGCCATTTTCTTTATACATGTTATGCCATCACTACCAACTACCAACTGGTGTCATTGCTTCACGGCCAGGTCCAATGCTAGCCGGTTCTGGCCTTTTTTCAGCAACAGTCAAAGGAAAAGGTGGGCATGCAGCTGCTCCACATAAGAATAAGGACTCGATTCTTGCAGCAGCATCAGCTGTTGTGGCTCTTCAGCAGATTGTTTCAAGAGAGACAAATCCTCTTGAGGCCGGGGTGGTGACCGTTGGGTACTTAAAGGGAGGTGAAGCAGGAAATGTGATCCCTGATTCTGTGAACTTTGGGGGAACTTTCAGGAGCTTGACAATGGAAGGACTCAGCCATATCCAAGAAAGGATCAAAGAGGTCATAGAACTGCAATCAGCCGTGCATCGATGTGAGGGCACGGTGGACTTTATGGAGGAAACACCGCTGCCTTATCCAGTGATGATTAACAATGAAGCATTGTATGAGCATGCAAAGAAGGTAGGTGAGGCCCTACTCGGGGAACCTAATGTGGAGCTTCACCCATTGATCATGGGAACAGAGGATTTCAGCTTCTACTCGCATAAGTTTGCGGCTGCAATTTTCTTTCTCGGGATAAAGAATGAAAGTCTAAAATCGGACCAGCCATTACACTCGTCTCTATTTGTCATCGACGAACAGGCTTCTTCTGTAGGAGCTGCTCTCAATGCTGCTGTTGCAATATCTTACTTGGATACCCATGATGTTGGGAACTTATTAAAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010210 GO:0010211 GO:0010817 GO:0012505 GO:0016787 GO:0016810 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

423

Amino Acids

46.36

Weight (kDa)

5.75

Isoelectric Point (pI)

44.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	101 - 150	1.5e-14	Peptidase family M20/M25/M40
M20_dimer	PF07687	202 - 297	3.1e-11	Peptidase dimerisation domain
Peptidase_M20	PF01546	256 - 376	1.2e-06	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02875 AT3G02875 AT3G02875
fragaria_vesca	FvH4_6g19670 FvH4_6g19670 FvH4_6g19670 FvH4_6g19670 FvH4_6g19690 FvH4_6g19700 FvH4_6g19700
malus_domestica	MD14G1032600.v1.1 MD14G1032900.v1.1 MD14G1033000.v1.1 MD14G1033100.v1.1
prunus_persica	Prupe.7G099900_v2.0.a1 Prupe.7G100000_v2.0.a1 Prupe.7G100000_v2.0.a1
pyrus_communis	pycom14g02930 pycom14g02940
rosa_chinensis	RchiOBHm_Chr3g0475011 RchiOBHm_Chr3g0475021 RchiOBHm_Chr3g0475031
rosa_laevigata	RLG00000023869 RLG00000023870 RLG00000023871
rosa_multiflora	Rmu_sc0003093.1_g000010 Rmu_sc0003093.1_g000011 Rmu_sc0003093.1_g000012 Rmu_sc0003093.1_g000017
rosa_roxburghii	Rroxscaffold_6G00406610 Rroxscaffold_6G00406620 Rroxscaffold_6G00406630
rosa_rugosa	Rorug03G0146000 Rorug03G0146100 Rorug03G0146200 Rorug03G0146300 Rorug03G0146300 Rorug03G0146300
rosa_samantha	Rh3AG195700 Rh3AG195900 Rh3AG196000 Rh3BG225900 Rh3BG226100 Rh3BG226200 Rh3CG220700 Rh3CG221000 Rh3CG221200 Rh3DG221200 Rh3DG221300 Rh3DG221400
rosa_wichuraiana	Rw3G017820 Rw3G017830 Rw3G017840 Rw3G017860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	170
AciI	CCGC	2 cut(s)	947, 1094
AclWI	GGATC	2 cut(s)	793, 881
AcoI	YGGCCR	1 cut(s)	584
AcuI	CTGAAG	2 cut(s)	317, 695
AcvI	CACGTG	1 cut(s)	240
AcyI	GRCGYC	1 cut(s)	321
AfaI	GTAC	3 cut(s)	161, 236, 770
AfiI	CCNNNNNNNGG	5 cut(s)	43, 170, 334, 1020, 1021
AflIII	ACRYGT	2 cut(s)	237, 537
AgsI	TTSAA	2 cut(s)	358, 726
AjnI	CCWGG	1 cut(s)	586
AleI	CACNNNNGTG	1 cut(s)	387
AloI	GAACNNNNNNTCC	2 cut(s)	101, 133
Alw21I	GWGCWC	3 cut(s)	72, 186, 216
Alw26I	GTCTC	4 cut(s)	56, 444, 725, 1164
AlwI	GGATC	2 cut(s)	793, 881
Ama87I	CYCGRG	4 cut(s)	71, 84, 1019, 1112
AoxI	GGCC	7 cut(s)	34, 111, 242, 584, 610, 747, 1011
ApeKI	GCWGC	8 cut(s)	650, 653, 686, 689, 949, 1097, 1199, 1211
AseI	ATTAAT	1 cut(s)	17
AspLEI	GCGC	1 cut(s)	425
AspS9I	GGNCC	4 cut(s)	34, 590, 1012, 1141
AsuC2I	CCSGG	1 cut(s)	751
AsuHPI	GGTGA	4 cut(s)	769, 794, 1019, 1034
AsuNHI	GCTAGC	1 cut(s)	598
AvaI	CYCGRG	4 cut(s)	71, 84, 1019, 1112
AvaII	GGWCC	2 cut(s)	590, 1141
BaeGI	GKGCMC	1 cut(s)	923
BanII	GRGCYC	2 cut(s)	72, 216
BbrPI	CACGTG	1 cut(s)	240
Bbv12I	GWGCWC	3 cut(s)	72, 186, 216
BbvI	GCAGC	8 cut(s)	640, 662, 698, 701, 936, 1084, 1186, 1198
BccI	CCATC	1 cut(s)	555
BceAI	ACGGC	2 cut(s)	599, 887
BcgI	CGANNNNNNTGC	2 cut(s)	502, 536
BciT130I	CCWGG	1 cut(s)	588
BciVI	GTATCC	1 cut(s)	1228
BclI	TGATCA	1 cut(s)	1050
BcnI	CCSGG	1 cut(s)	751
BcoDI	GTCTC	4 cut(s)	56, 444, 725, 1164
BfaI	CTAG	2 cut(s)	224, 599
BfmI	CTRYAG	1 cut(s)	1191
BfuI	GTATCC	1 cut(s)	1228
BisI	GCNGC	9 cut(s)	651, 654, 687, 690, 950, 1095, 1098, 1200, 1212
BlsI	GCNGC	9 cut(s)	652, 655, 688, 691, 951, 1096, 1099, 1201, 1213
Bme1390I	CCNGG	2 cut(s)	588, 751
Bme18I	GGWCC	2 cut(s)	590, 1141
BmeT110I	CYCGRG	4 cut(s)	71, 84, 1019, 1112
BmgT120I	GGNCC	4 cut(s)	34, 590, 1012, 1141
BmiI	GGNNCC	2 cut(s)	286, 1026
BmrFI	CCNGG	2 cut(s)	588, 751
BmsI	GCATC	4 cut(s)	107, 510, 701, 916
BmtI	GCTAGC	1 cut(s)	602
BplI	GAGNNNNNCTC	2 cut(s)	1188, 1220
BpuEI	CTTGAG	1 cut(s)	764
BpuMI	CCSGG	1 cut(s)	751
Bsa29I	ATCGAT	1 cut(s)	910
BsaAI	YACGTR	1 cut(s)	240
BsaHI	GRCGYC	1 cut(s)	321
BsaJI	CCNNGG	1 cut(s)	750
BsaXI	ACNNNNNCTCC	2 cut(s)	101, 131
Bsc4I	CCNNNNNNNGG	5 cut(s)	43, 170, 334, 1020, 1021
Bse118I	RCCGGY	1 cut(s)	602
Bse1I	ACTGG	6 cut(s)	57, 157, 262, 293, 571, 959
Bse3DI	GCAATG	2 cut(s)	417, 573
BseBI	CCWGG	1 cut(s)	588
BseCI	ATCGAT	1 cut(s)	910
BseDI	CCNNGG	1 cut(s)	750
BseGI	GGATG	1 cut(s)	135
BseLI	CCNNNNNNNGG	5 cut(s)	43, 170, 334, 1020, 1021
BseMI	GCAATG	2 cut(s)	417, 573
BseMII	CTCAG	2 cut(s)	319, 868
BseNI	ACTGG	6 cut(s)	57, 157, 262, 293, 571, 959
BseRI	GAGGAG	3 cut(s)	157, 160, 194
BseSI	GKGCMC	1 cut(s)	923
BseXI	GCAGC	8 cut(s)	640, 662, 698, 701, 936, 1084, 1186, 1198
BshFI	GGCC	7 cut(s)	36, 113, 244, 586, 612, 749, 1013
BshVI	ATCGAT	1 cut(s)	910
BsiHKAI	GWGCWC	3 cut(s)	72, 186, 216
BsiHKCI	CYCGRG	4 cut(s)	71, 84, 1019, 1112
BsiSI	CCGG	3 cut(s)	476, 603, 750
BslI	CCNNNNNNNGG	5 cut(s)	43, 170, 334, 1020, 1021
BsmAI	GTCTC	4 cut(s)	56, 444, 725, 1164
BsmBI	CGTCTC	1 cut(s)	1164
BsmI	GAATGC	1 cut(s)	503
BsnI	GGCC	7 cut(s)	36, 113, 244, 586, 612, 749, 1013
BsoBI	CYCGRG	4 cut(s)	71, 84, 1019, 1112
Bsp1286I	GDGCHC	4 cut(s)	72, 186, 216, 923
Bsp143I	GATC	3 cut(s)	798, 873, 1050
BspACI	CCGC	2 cut(s)	947, 1094
BspANI	GGCC	7 cut(s)	36, 113, 244, 586, 612, 749, 1013
BspCNI	CTCAG	2 cut(s)	318, 867
BspDI	ATCGAT	1 cut(s)	910
BspLI	GGNNCC	2 cut(s)	286, 1026
BspOI	GCTAGC	1 cut(s)	602
BspPI	GGATC	2 cut(s)	793, 881
BspQI	GCTCTTC	1 cut(s)	714
BsrDI	GCAATG	2 cut(s)	417, 573
BsrFI	RCCGGY	1 cut(s)	602
BsrI	ACTGG	6 cut(s)	57, 157, 262, 293, 571, 959
BssAI	RCCGGY	1 cut(s)	602
BssECI	CCNNGG	1 cut(s)	750
BssMI	GATC	3 cut(s)	798, 873, 1050
BssNI	GRCGYC	1 cut(s)	321
Bst2UI	CCWGG	1 cut(s)	588
Bst4CI	ACNGT	3 cut(s)	628, 763, 925
Bst6I	CTCTTC	3 cut(s)	336, 458, 714
BstACI	GRCGYC	1 cut(s)	321
BstBAI	YACGTR	1 cut(s)	240
BstC8I	GCNNGC	4 cut(s)	384, 600, 648, 993
BstDEI	CTNAG	2 cut(s)	305, 854
BstEII	GGTNACC	1 cut(s)	757
BstF5I	GGATG	1 cut(s)	135
BstHHI	GCGC	1 cut(s)	425
BstKTI	GATC	3 cut(s)	801, 876, 1053
BstMAI	GTCTC	4 cut(s)	56, 444, 725, 1164
BstMBI	GATC	3 cut(s)	798, 873, 1050
BstMWI	GCNNNNNNNGC	8 cut(s)	76, 110, 220, 308, 695, 704, 1208, 1217
BstNI	CCWGG	1 cut(s)	588
BstNSI	RCATGY	4 cut(s)	390, 541, 650, 995
BstPI	GGTNACC	1 cut(s)	757
BstSCI	CCNGG	2 cut(s)	586, 749
BstSFI	CTRYAG	1 cut(s)	1191
BstSLI	GKGCMC	1 cut(s)	923
BstV1I	GCAGC	8 cut(s)	640, 662, 698, 701, 936, 1084, 1186, 1198
BstXI	CCANNNNNNTGG	1 cut(s)	1247
Bsu15I	ATCGAT	1 cut(s)	910
BsuI	GTATCC	1 cut(s)	1228
BsuRI	GGCC	7 cut(s)	36, 113, 244, 586, 612, 749, 1013
BsuTUI	ATCGAT	1 cut(s)	910
BtsCI	GGATG	1 cut(s)	135
BtsIMutI	CAGTG	3 cut(s)	64, 300, 966
Cac8I	GCNNGC	4 cut(s)	384, 600, 648, 993
CfoI	GCGC	1 cut(s)	425
Cfr10I	RCCGGY	1 cut(s)	602
Cfr13I	GGNCC	4 cut(s)	34, 590, 1012, 1141
ClaI	ATCGAT	1 cut(s)	910
CseI	GACGC	1 cut(s)	329
Csp6I	GTAC	3 cut(s)	160, 235, 769
CspCI	CAANNNNNGTGG	2 cut(s)	329, 364
CviQI	GTAC	3 cut(s)	160, 235, 769
DdeI	CTNAG	2 cut(s)	305, 854
DpnI	GATC	3 cut(s)	800, 875, 1052
DpnII	GATC	3 cut(s)	798, 873, 1050
EaeI	YGGCCR	1 cut(s)	584
Eam1104I	CTCTTC	3 cut(s)	336, 458, 714
EarI	CTCTTC	3 cut(s)	336, 458, 714
Ecl136II	GAGCTC	2 cut(s)	70, 214
Eco24I	GRGCYC	2 cut(s)	72, 216
Eco47I	GGWCC	2 cut(s)	590, 1141
Eco53kI	GAGCTC	2 cut(s)	70, 214
Eco57I	CTGAAG	2 cut(s)	317, 695
Eco72I	CACGTG	1 cut(s)	240
Eco88I	CYCGRG	4 cut(s)	71, 84, 1019, 1112
Eco91I	GGTNACC	1 cut(s)	757
EcoICRI	GAGCTC	2 cut(s)	70, 214
EcoO109I	RGGNCCY	1 cut(s)	1012
EcoO65I	GGTNACC	1 cut(s)	757
EcoRII	CCWGG	1 cut(s)	586
EcoT38I	GRGCYC	2 cut(s)	72, 216
Esp3I	CGTCTC	1 cut(s)	1164
FauNDI	CATATG	1 cut(s)	488
FbaI	TGATCA	1 cut(s)	1050
Fnu4HI	GCNGC	9 cut(s)	651, 654, 687, 690, 950, 1095, 1098, 1200, 1212
FokI	GGATG	1 cut(s)	142
FriOI	GRGCYC	2 cut(s)	72, 216
Fsp4HI	GCNGC	9 cut(s)	651, 654, 687, 690, 950, 1095, 1098, 1200, 1212
FspBI	CTAG	2 cut(s)	224, 599
GlaI	GCGC	1 cut(s)	424
GluI	GCNGC	9 cut(s)	651, 654, 687, 690, 950, 1095, 1098, 1200, 1212
HaeIII	GGCC	7 cut(s)	36, 113, 244, 586, 612, 749, 1013
HapII	CCGG	3 cut(s)	476, 603, 750
HgaI	GACGC	1 cut(s)	329
HhaI	GCGC	1 cut(s)	425
Hin1I	GRCGYC	1 cut(s)	321
Hin6I	GCGC	1 cut(s)	423
HinP1I	GCGC	1 cut(s)	423
HinfI	GANTC	7 cut(s)	82, 150, 398, 674, 679, 806, 852
HpaII	CCGG	3 cut(s)	476, 603, 750
HphI	GGTGA	4 cut(s)	769, 794, 1019, 1034
Hpy166II	GTNNAC	3 cut(s)	237, 814, 928
Hpy188I	TCNGA	2 cut(s)	52, 1141
Hpy188III	TCNNGA	9 cut(s)	86, 205, 335, 358, 395, 726, 743, 832, 1114
Hpy8I	GTNNAC	3 cut(s)	237, 814, 928
Hpy99I	CGWCG	1 cut(s)	1178
HpyAV	CCTTC	3 cut(s)	100, 842, 994
HpyCH4III	ACNGT	3 cut(s)	628, 763, 925
HpyCH4IV	ACGT	1 cut(s)	239
HpyCH4V	TGCA	9 cut(s)	22, 382, 650, 686, 895, 907, 995, 1100, 1220
HpyF10VI	GCNNNNNNNGC	8 cut(s)	76, 110, 220, 308, 695, 704, 1208, 1217
HpyF3I	CTNAG	2 cut(s)	305, 854
HpySE526I	ACGT	1 cut(s)	239
Hsp92I	GRCGYC	1 cut(s)	321
HspAI	GCGC	1 cut(s)	423
Ksp22I	TGATCA	1 cut(s)	1050
Kzo9I	GATC	3 cut(s)	798, 873, 1050
LguI	GCTCTTC	1 cut(s)	714
LmnI	GCTCC	9 cut(s)	88, 181, 211, 290, 478, 661, 834, 1036, 1196
Lsp1109I	GCAGC	8 cut(s)	640, 662, 698, 701, 936, 1084, 1186, 1198
LweI	GCATC	4 cut(s)	107, 510, 701, 916
MaeI	CTAG	2 cut(s)	224, 599
MaeII	ACGT	1 cut(s)	239
MaeIII	GTNAC	4 cut(s)	392, 432, 491, 757
MalI	GATC	3 cut(s)	800, 875, 1052
MboI	GATC	3 cut(s)	798, 873, 1050
MboII	GAAGA	4 cut(s)	323, 475, 701, 1179
MhlI	GDGCHC	4 cut(s)	72, 186, 216, 923
MluCI	AATT	2 cut(s)	228, 1101
MlyI	GAGTC	3 cut(s)	76, 668, 846
MseI	TTAA	5 cut(s)	17, 509, 773, 969, 1259
MslI	CAYNNNNRTG	2 cut(s)	387, 912
MspA1I	CMGCKG	3 cut(s)	653, 698, 949
MspI	CCGG	3 cut(s)	476, 603, 750
MspR9I	CCNGG	2 cut(s)	588, 751
Mva1269I	GAATGC	1 cut(s)	503
MvaI	CCWGG	1 cut(s)	588
MwoI	GCNNNNNNNGC	8 cut(s)	76, 110, 220, 308, 695, 704, 1208, 1217
NciI	CCSGG	1 cut(s)	751
NdeI	CATATG	1 cut(s)	488
NdeII	GATC	3 cut(s)	798, 873, 1050
NheI	GCTAGC	1 cut(s)	598
NlaIV	GGNNCC	2 cut(s)	286, 1026
NmeAIII	GCCGAG	1 cut(s)	139
NmuCI	GTSAC	2 cut(s)	392, 757
NspI	RCATGY	4 cut(s)	390, 541, 650, 995
OliI	CACNNNNGTG	1 cut(s)	387
PaeI	GCATGC	2 cut(s)	650, 995
PaeR7I	CTCGAG	1 cut(s)	71
PciI	ACATGT	1 cut(s)	537
PciSI	GCTCTTC	1 cut(s)	714
PctI	GAATGC	1 cut(s)	503
PfeI	GAWTC	4 cut(s)	150, 398, 679, 806
PflMI	CCANNNNNTGG	1 cut(s)	170
PkrI	GCNGC	9 cut(s)	652, 655, 688, 691, 951, 1096, 1099, 1201, 1213
PleI	GAGTC	3 cut(s)	76, 668, 846
PmaCI	CACGTG	1 cut(s)	240
PmlI	CACGTG	1 cut(s)	240
PpsI	GAGTC	3 cut(s)	76, 668, 846
Ppu21I	YACGTR	1 cut(s)	240
PscI	ACATGT	1 cut(s)	537
PshBI	ATTAAT	1 cut(s)	17
Psp124BI	GAGCTC	2 cut(s)	72, 216
Psp6I	CCWGG	1 cut(s)	586
PspCI	CACGTG	1 cut(s)	240
PspEI	GGTNACC	1 cut(s)	757
PspGI	CCWGG	1 cut(s)	586
PspN4I	GGNNCC	2 cut(s)	286, 1026
PspPI	GGNCC	4 cut(s)	34, 590, 1012, 1141
PspXI	VCTCGAGB	1 cut(s)	71
PvuII	CAGCTG	2 cut(s)	653, 698
RsaI	GTAC	3 cut(s)	161, 236, 770
RsaNI	GTAC	3 cut(s)	160, 235, 769
RseI	CAYNNNNRTG	2 cut(s)	387, 912
SacI	GAGCTC	2 cut(s)	72, 216
SapI	GCTCTTC	1 cut(s)	714
SaqAI	TTAA	5 cut(s)	17, 509, 773, 969, 1259
SatI	GCNGC	9 cut(s)	651, 654, 687, 690, 950, 1095, 1098, 1200, 1212
Sau3AI	GATC	3 cut(s)	798, 873, 1050
Sau96I	GGNCC	4 cut(s)	34, 590, 1012, 1141
SchI	GAGTC	3 cut(s)	76, 668, 846
ScrFI	CCNGG	2 cut(s)	588, 751
SduI	GDGCHC	4 cut(s)	72, 186, 216, 923
SfaNI	GCATC	4 cut(s)	107, 510, 701, 916
SfcI	CTRYAG	1 cut(s)	1191
Sfr274I	CTCGAG	1 cut(s)	71
SinI	GGWCC	2 cut(s)	590, 1141
SlaI	CTCGAG	1 cut(s)	71
SmiMI	CAYNNNNRTG	2 cut(s)	387, 912
SmlI	CTYRAG	2 cut(s)	71, 743
SmoI	CTYRAG	2 cut(s)	71, 743
SphI	GCATGC	2 cut(s)	650, 995
Sse9I	AATT	2 cut(s)	228, 1101
SsiI	CCGC	2 cut(s)	947, 1094
SspMI	CTAG	2 cut(s)	224, 599
SstI	GAGCTC	2 cut(s)	72, 216
StyD4I	CCNGG	2 cut(s)	586, 749
TaaI	ACNGT	3 cut(s)	628, 763, 925
TaiI	ACGT	1 cut(s)	242
TaqI	TCGA	6 cut(s)	72, 177, 216, 677, 910, 1173
TasI	AATT	2 cut(s)	228, 1101
TatI	WGTACW	1 cut(s)	234
TauI	GCSGC	1 cut(s)	1097
TfiI	GAWTC	4 cut(s)	150, 398, 679, 806
Tru1I	TTAA	5 cut(s)	17, 509, 773, 969, 1259
Tru9I	TTAA	5 cut(s)	17, 509, 773, 969, 1259
TscAI	CASTG	3 cut(s)	64, 300, 966
TseFI	GTSAC	2 cut(s)	392, 757
TseI	GCWGC	8 cut(s)	650, 653, 686, 689, 949, 1097, 1199, 1211
Tsp45I	GTSAC	2 cut(s)	392, 757
TspDTI	ATGAA	2 cut(s)	990, 1140
TspGWI	ACGGA	1 cut(s)	224
TspRI	CASTG	3 cut(s)	64, 300, 966
Van91I	CCANNNNNTGG	1 cut(s)	170
VpaK11BI	GGWCC	2 cut(s)	590, 1141
VspI	ATTAAT	1 cut(s)	17
XceI	RCATGY	4 cut(s)	390, 541, 650, 995
XcmI	CCANNNNNNNNNTGG	2 cut(s)	259, 563
XhoI	CTCGAG	1 cut(s)	71
XspI	CTAG	2 cut(s)	224, 599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.