Rorug03G0146300

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001332
Physical Location & Seq
Reverse (-)
11973211 .. 11976693
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0146300.1

Sequence Viewer

Length: 1581 bp
ATGAATCAGGGCTTTTGGATGGCAAAGGGTACCGGGGGTGTAAATGATGGTGAGAGGCTTTATGATAATTCTTCTGCAACTGAGCCTAAGCGTTCTCATCAATGGTTCATGGATGGTCCTGAGGTAGAGTTACTCCCAAACAAGAAACAGGCTATTGAAGTTCCAGGCACCAATCTATTTTCAGGCTTGTTGAACGCAAATGTTTCTCCATGGGGAAATGTTCCCAGTTTTCAGTCATTCTCTGGCCAGTTCACTGAAAGGTCATTTGATTCTGAAGCAGACAGAGCCGTGAATTTTGAGGACAGAAACATTTCATCAGTTGGCTCAGAAAAAATGAGAAAAATTAATGAGGATCTATTTGGGAATGATTCTTCTTTTGGTTTATCTATGTCCCATTCACTGGAAGATCCTAGGTTGAGTCCTAACTATGGTGGTTTTAGAAAAGTGAAAGTCAGTGAGGTAAAGGACTCTGAGAATGTCATGCGTGTTTCGGTTGGACATGCTTATAATCCGGGAGATAACGATGCCATGTTGGCGCCTCATGTTTACAAGGCGGATGACAACTCAGGATCAATGGGTCTCACTTATAAGAAAGAGGATGAGAACTTCATAACCATGAGTGATTCCTATAATAGGGCAGATAACAATTTTAGATCAATGGGACAACCCTTTACTAAAGGGGATGAGTACATAGGAATTGGTCAAACATACAAGGAGGATAGTAATACCCTATTGAGGAGTCGAACATTCAACAAGGGTGACAGTACTATCATCTCGGTTGGTGAAACCTACAACAAGGCAGATGAAAGTACCATGTCAACTGGTCATATTTATAATAAAGGGGAAGAGACATCAATGGGTCATGACTATAGCAAGGTCGACAATAATATGTTGTCAATTGGTCACTCTTACAATAAGGGAGAGAGTACTATCATATCCTTTGGCGGGTGTGATGATGATGATGATGTAAATACCTCAACAATCTCTGGCTATGAACTTCTTATGGGTCAATCTTTTCCAAAAACAGAAGCTATGAATGAGAAAGACTTGGGTAAATTAAATGCTGATGCATTTGTAAATCAACCTCATATAATTGCTGGAGCTGAAACTGTTTCCAGAAAGAAGGATGACCAGAAAATGTCTAAGAAGTCTCCTTCAAACAACTTCCCTTCGAATGTGCGAAGTTTGCTATCTACTGGTATGCTGGATGGAGTTCCTGTAAAGTATATTGCCTGGTCACGGGAGGAGCTGCAGGCTGTAATTAAAGGTTCTGGGTATCTATGTGGCTGTCAGTCATGTAACTTCTCTAAGGTAATCAATGCATATGAATTTGAGCGTCATGCTAGTTGCAAAACAAAACACCCAAATAACCACATATATTTTGAGAATGGGAAGACCATCTATGGGATTGTGCAAGAGCTCAGGAGCACACCTCAGAATATGCTGTTTGACGTTATTCAGACTATTACTGGTTCACAAATCAATCAGAAATCCTTCCGTCTTTGGAAAGAATCGTTTTTGGCAGCAACACGTGAACTTCAACGTATATATGGCAATGATGAAGTGAAACAATTGGCATGA

Protein Analysis

526

Amino Acids

58.6

Weight (kDa)

5.45

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 420 - 473 2.7e-13 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 507, 588, 834
Acc65I GGTACC 1 cut(s) 29
AccB1I GGYRCC 3 cut(s) 29, 167, 535
AccB7I CCANNNNNTGG 1 cut(s) 400
AccI GTMKAC 1 cut(s) 879
AciI CCGC 2 cut(s) 554, 945
AclWI GGATC 3 cut(s) 360, 401, 577
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 2 cut(s) 292, 1328
AcuI CTGAAG 1 cut(s) 294
AcvI CACGTG 1 cut(s) 1532
AcyI GRCGYC 1 cut(s) 536
AfaI GTAC 5 cut(s) 31, 689, 766, 811, 928
AfiI CCNNNNNNNGG 7 cut(s) 400, 428, 633, 735, 945, 1239, 1404
AflIII ACRYGT 1 cut(s) 1529
AgsI TTSAA 5 cut(s) 158, 193, 751, 1158, 1541
AjnI CCWGG 2 cut(s) 163, 1232
AluBI AGCT 4 cut(s) 1031, 1103, 1249, 1420
AluI AGCT 4 cut(s) 1031, 1103, 1249, 1420
Alw21I GWGCWC 2 cut(s) 1422, 1430
Alw26I GTCTC 3 cut(s) 584, 842, 1155
AlwI GGATC 3 cut(s) 360, 401, 577
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 1249, 1523
ApoI RAATTY 2 cut(s) 292, 1328
AseI ATTAAT 1 cut(s) 345
Asp700I GAANNNNTTC 2 cut(s) 310, 1493
Asp718I GGTACC 1 cut(s) 29
AspA2I CCTAGG 1 cut(s) 410
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 2 cut(s) 34, 513
AsuHPI GGTGA 3 cut(s) 62, 770, 794
AsuII TTCGAA 1 cut(s) 1172
AvaII GGWCC 1 cut(s) 116
AvrII CCTAGG 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 120
BalI TGGCCA 1 cut(s) 246
BanI GGYRCC 3 cut(s) 29, 167, 535
BanII GRGCYC 1 cut(s) 1422
BbrPI CACGTG 1 cut(s) 1532
BbsI GAAGAC 1 cut(s) 1400
Bbv12I GWGCWC 2 cut(s) 1422, 1430
BbvI GCAGC 2 cut(s) 1236, 1535
BccI CCATC 5 cut(s) 13, 41, 107, 1202, 1406
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 2 cut(s) 165, 1234
BcnI CCSGG 2 cut(s) 34, 513
BcoDI GTCTC 3 cut(s) 584, 842, 1155
BfaI CTAG 2 cut(s) 411, 1344
BfmI CTRYAG 2 cut(s) 868, 1250
BfoI RGCGCY 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 533
BisI GCNGC 2 cut(s) 1250, 1524
BlnI CCTAGG 1 cut(s) 410
BlsI GCNGC 2 cut(s) 1251, 1525
BmcAI AGTACT 2 cut(s) 766, 928
Bme1390I CCNGG 4 cut(s) 34, 165, 513, 1234
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 3 cut(s) 31, 169, 537
BmrFI CCNGG 4 cut(s) 34, 165, 513, 1234
BmrI ACTGGG 1 cut(s) 219
BmsI GCATC 2 cut(s) 514, 1057
BmuI ACTGGG 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 1400
BplI GAGNNNNNCTC 2 cut(s) 1417, 1449
BpmI CTGGAG 1 cut(s) 1119
Bpu10I CCTNAGC 2 cut(s) 87, 1421
Bpu14I TTCGAA 1 cut(s) 1172
BpuMI CCSGG 2 cut(s) 34, 513
BsaAI YACGTR 1 cut(s) 1532
BsaHI GRCGYC 1 cut(s) 536
BsaI GGTCTC 1 cut(s) 584
BsaJI CCNNGG 3 cut(s) 33, 209, 410
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bsc4I CCNNNNNNNGG 7 cut(s) 400, 428, 633, 735, 945, 1239, 1404
Bse1I ACTGG 6 cut(s) 225, 247, 405, 826, 1201, 1474
Bse21I CCTNAGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 1561
BseBI CCWGG 2 cut(s) 165, 1234
BseDI CCNNGG 3 cut(s) 33, 209, 410
BseGI GGATG 7 cut(s) 24, 118, 562, 604, 688, 1132, 1213
BseLI CCNNNNNNNGG 7 cut(s) 400, 428, 633, 735, 945, 1239, 1404
BseMI GCAATG 1 cut(s) 1561
BseMII CTCAG 7 cut(s) 72, 111, 339, 462, 579, 1435, 1448
BseNI ACTGG 6 cut(s) 225, 247, 405, 826, 1201, 1474
BseRI GAGGAG 2 cut(s) 751, 1259
BseXI GCAGC 2 cut(s) 1236, 1535
BshFI GGCC 1 cut(s) 246
BshNI GGYRCC 3 cut(s) 29, 167, 535
BsiHKAI GWGCWC 2 cut(s) 1422, 1430
BsiSI CCGG 2 cut(s) 33, 512
BslFI GGGAC 2 cut(s) 376, 675
BslI CCNNNNNNNGG 7 cut(s) 400, 428, 633, 735, 945, 1239, 1404
BsmAI GTCTC 3 cut(s) 584, 842, 1155
BsmFI GGGAC 2 cut(s) 376, 675
BsnI GGCC 1 cut(s) 246
Bso31I GGTCTC 1 cut(s) 584
Bsp119I TTCGAA 1 cut(s) 1172
Bsp1286I GDGCHC 2 cut(s) 1422, 1430
Bsp143I GATC 4 cut(s) 352, 406, 569, 653
Bsp19I CCATGG 1 cut(s) 209
BspACI CCGC 2 cut(s) 554, 945
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 7 cut(s) 73, 112, 338, 463, 578, 1434, 1447
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 3 cut(s) 31, 169, 537
BspMAI CTGCAG 1 cut(s) 1254
BspPI GGATC 3 cut(s) 360, 401, 577
BspT104I TTCGAA 1 cut(s) 1172
BspT107I GGYRCC 3 cut(s) 29, 167, 535
BspTNI GGTCTC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 1561
BsrI ACTGG 6 cut(s) 225, 247, 405, 826, 1201, 1474
BssECI CCNNGG 3 cut(s) 33, 209, 410
BssMI GATC 4 cut(s) 352, 406, 569, 653
BssNI GRCGYC 1 cut(s) 536
BssT1I CCWWGG 2 cut(s) 209, 410
Bst2UI CCWGG 2 cut(s) 165, 1234
Bst4CI ACNGT 2 cut(s) 764, 1111
Bst6I CTCTTC 1 cut(s) 840
BstACI GRCGYC 1 cut(s) 536
BstBAI YACGTR 1 cut(s) 1532
BstBI TTCGAA 1 cut(s) 1172
BstC8I GCNNGC 1 cut(s) 1254
BstDSI CCRYGG 1 cut(s) 209
BstENI CCTNNNNNAGG 1 cut(s) 631
BstF5I GGATG 7 cut(s) 24, 118, 562, 604, 688, 1132, 1213
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 4 cut(s) 355, 409, 572, 656
BstMAI GTCTC 3 cut(s) 584, 842, 1155
BstMBI GATC 4 cut(s) 352, 406, 569, 653
BstMWI GCNNNNNNNGC 3 cut(s) 284, 533, 1186
BstNI CCWGG 2 cut(s) 165, 1234
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 4 cut(s) 32, 163, 511, 1232
BstSFI CTRYAG 2 cut(s) 868, 1250
BstV1I GCAGC 2 cut(s) 1236, 1535
BstV2I GAAGAC 1 cut(s) 1400
BstX2I RGATCY 2 cut(s) 352, 406
BstYI RGATCY 2 cut(s) 352, 406
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 7 cut(s) 24, 118, 562, 604, 688, 1132, 1213
BtsIMutI CAGTG 3 cut(s) 252, 398, 460
Cac8I GCNNGC 1 cut(s) 1254
CciI TCATGA 1 cut(s) 862
CfoI GCGC 1 cut(s) 538
Cfr13I GGNCC 1 cut(s) 116
CseI GACGC 1 cut(s) 1325
Csp6I GTAC 5 cut(s) 30, 688, 765, 810, 927
CviQI GTAC 5 cut(s) 30, 688, 765, 810, 927
DinI GGCGCC 1 cut(s) 537
DpnI GATC 4 cut(s) 354, 408, 571, 655
DpnII GATC 4 cut(s) 352, 406, 569, 653
EaeI YGGCCR 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 840
EarI CTCTTC 1 cut(s) 840
EciI GGCGGA 1 cut(s) 569
Ecl136II GAGCTC 1 cut(s) 1420
Eco130I CCWWGG 2 cut(s) 209, 410
Eco24I GRGCYC 1 cut(s) 1422
Eco31I GGTCTC 1 cut(s) 584
Eco47I GGWCC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 1420
Eco57I CTGAAG 1 cut(s) 294
Eco72I CACGTG 1 cut(s) 1532
Eco81I CCTNAGG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 1420
EcoNI CCTNNNNNAGG 1 cut(s) 631
EcoRII CCWGG 2 cut(s) 163, 1232
EcoT14I CCWWGG 2 cut(s) 209, 410
EcoT22I ATGCAT 2 cut(s) 1072, 1324
EcoT38I GRGCYC 1 cut(s) 1422
EgeI GGCGCC 1 cut(s) 537
EheI GGCGCC 1 cut(s) 537
ErhI CCWWGG 2 cut(s) 209, 410
FaqI GGGAC 2 cut(s) 376, 675
FauI CCCGC 1 cut(s) 938
FauNDI CATATG 1 cut(s) 1324
FblI GTMKAC 1 cut(s) 879
Fnu4HI GCNGC 2 cut(s) 1250, 1524
FokI GGATG 7 cut(s) 31, 125, 569, 611, 695, 1139, 1220
FriOI GRGCYC 1 cut(s) 1422
Fsp4HI GCNGC 2 cut(s) 1250, 1524
FspBI CTAG 2 cut(s) 411, 1344
GlaI GCGC 1 cut(s) 537
GluI GCNGC 2 cut(s) 1250, 1524
GsuI CTGGAG 1 cut(s) 1119
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 1 cut(s) 246
HapII CCGG 2 cut(s) 33, 512
HgaI GACGC 1 cut(s) 1325
HhaI GCGC 1 cut(s) 538
Hin1I GRCGYC 1 cut(s) 536
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HincII GTYRAC 2 cut(s) 819, 880
HindII GTYRAC 2 cut(s) 819, 880
HinfI GANTC 8 cut(s) 4, 269, 368, 418, 467, 623, 739, 1511
HpaII CCGG 2 cut(s) 33, 512
HphI GGTGA 3 cut(s) 62, 770, 794
Hpy166II GTNNAC 6 cut(s) 252, 547, 819, 880, 1475, 1535
Hpy188I TCNGA 6 cut(s) 274, 328, 472, 1437, 1461, 1488
Hpy188III TCNNGA 5 cut(s) 119, 567, 863, 1116, 1423
Hpy8I GTNNAC 6 cut(s) 252, 547, 819, 880, 1475, 1535
HpyAV CCTTC 4 cut(s) 1117, 1164, 1179, 1504
HpyCH4III ACNGT 2 cut(s) 764, 1111
HpyCH4IV ACGT 3 cut(s) 1452, 1531, 1543
HpyCH4V TGCA 6 cut(s) 77, 1070, 1252, 1322, 1350, 1414
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 533, 1186
HpySE526I ACGT 3 cut(s) 1452, 1531, 1543
Hsp92I GRCGYC 1 cut(s) 536
HspAI GCGC 1 cut(s) 536
KasI GGCGCC 1 cut(s) 535
KpnI GGTACC 1 cut(s) 33
Kzo9I GATC 4 cut(s) 352, 406, 569, 653
LmnI GCTCC 3 cut(s) 1100, 1246, 1425
Lsp1109I GCAGC 2 cut(s) 1236, 1535
LweI GCATC 2 cut(s) 514, 1057
MaeI CTAG 2 cut(s) 411, 1344
MaeII ACGT 3 cut(s) 1452, 1531, 1543
MaeIII GTNAC 5 cut(s) 129, 758, 902, 1236, 1298
MalI GATC 4 cut(s) 354, 408, 571, 655
MboI GATC 4 cut(s) 352, 406, 569, 653
MboII GAAGA 5 cut(s) 63, 363, 416, 857, 1405
MfeI CAATTG 2 cut(s) 897, 1571
MflI RGATCY 2 cut(s) 352, 406
MhlI GDGCHC 2 cut(s) 1422, 1430
MlsI TGGCCA 1 cut(s) 246
MluNI TGGCCA 1 cut(s) 246
Mly113I GGCGCC 1 cut(s) 536
MlyI GAGTC 3 cut(s) 427, 461, 748
MmeI TCCRAC 1 cut(s) 475
Mox20I TGGCCA 1 cut(s) 246
Mph1103I ATGCAT 2 cut(s) 1072, 1324
MroXI GAANNNNTTC 2 cut(s) 310, 1493
MscI TGGCCA 1 cut(s) 246
MseI TTAA 3 cut(s) 345, 1058, 1263
MslI CAYNNNNRTG 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 246
MspI CCGG 2 cut(s) 33, 512
MspR9I CCNGG 4 cut(s) 34, 165, 513, 1234
MunI CAATTG 2 cut(s) 897, 1571
MvaI CCWGG 2 cut(s) 165, 1234
MwoI GCNNNNNNNGC 3 cut(s) 284, 533, 1186
NarI GGCGCC 1 cut(s) 536
NciI CCSGG 2 cut(s) 34, 513
NcoI CCATGG 1 cut(s) 209
NdeI CATATG 1 cut(s) 1324
NdeII GATC 4 cut(s) 352, 406, 569, 653
NlaIV GGNNCC 3 cut(s) 31, 169, 537
NmuCI GTSAC 3 cut(s) 758, 902, 1236
NsiI ATGCAT 2 cut(s) 1072, 1324
NspI RCATGY 1 cut(s) 503
NspV TTCGAA 1 cut(s) 1172
PagI TCATGA 1 cut(s) 862
PdmI GAANNNNTTC 2 cut(s) 310, 1493
PfeI GAWTC 5 cut(s) 4, 269, 368, 623, 1511
PflMI CCANNNNNTGG 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 2 cut(s) 1251, 1525
PleI GAGTC 3 cut(s) 426, 461, 747
PluTI GGCGCC 1 cut(s) 539
PmaCI CACGTG 1 cut(s) 1532
PmlI CACGTG 1 cut(s) 1532
PpsI GAGTC 3 cut(s) 426, 461, 747
Ppu21I YACGTR 1 cut(s) 1532
PshBI ATTAAT 1 cut(s) 345
PsiI TTATAA 3 cut(s) 507, 588, 834
Psp124BI GAGCTC 1 cut(s) 1422
Psp6I CCWGG 2 cut(s) 163, 1232
PspCI CACGTG 1 cut(s) 1532
PspGI CCWGG 2 cut(s) 163, 1232
PspN4I GGNNCC 3 cut(s) 31, 169, 537
PspPI GGNCC 1 cut(s) 116
PstI CTGCAG 1 cut(s) 1254
PsuI RGATCY 2 cut(s) 352, 406
RsaI GTAC 5 cut(s) 31, 689, 766, 811, 928
RsaNI GTAC 5 cut(s) 30, 688, 765, 810, 927
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 1 cut(s) 1422
SalI GTCGAC 1 cut(s) 878
SaqAI TTAA 3 cut(s) 345, 1058, 1263
SatI GCNGC 2 cut(s) 1250, 1524
Sau3AI GATC 4 cut(s) 352, 406, 569, 653
Sau96I GGNCC 1 cut(s) 116
ScaI AGTACT 2 cut(s) 766, 928
SchI GAGTC 3 cut(s) 427, 461, 748
ScrFI CCNGG 4 cut(s) 34, 165, 513, 1234
SduI GDGCHC 2 cut(s) 1422, 1430
SfaNI GCATC 2 cut(s) 514, 1057
SfcI CTRYAG 2 cut(s) 868, 1250
SfoI GGCGCC 1 cut(s) 537
SfuI TTCGAA 1 cut(s) 1172
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 614
SsiI CCGC 2 cut(s) 554, 945
SspDI GGCGCC 1 cut(s) 535
SspMI CTAG 2 cut(s) 411, 1344
SstI GAGCTC 1 cut(s) 1422
StyD4I CCNGG 4 cut(s) 32, 163, 511, 1232
StyI CCWWGG 2 cut(s) 209, 410
TaaI ACNGT 2 cut(s) 764, 1111
TaiI ACGT 3 cut(s) 1455, 1534, 1546
TaqI TCGA 3 cut(s) 742, 879, 1172
TatI WGTACW 3 cut(s) 687, 764, 926
TfiI GAWTC 5 cut(s) 4, 269, 368, 623, 1511
Tru1I TTAA 3 cut(s) 345, 1058, 1263
Tru9I TTAA 3 cut(s) 345, 1058, 1263
TscAI CASTG 3 cut(s) 259, 405, 460
TseFI GTSAC 3 cut(s) 758, 902, 1236
TseI GCWGC 2 cut(s) 1249, 1523
Tsp45I GTSAC 3 cut(s) 758, 902, 1236
TspDTI ATGAA 9 cut(s) 17, 97, 303, 598, 819, 1008, 1049, 1341, 1575
TspGWI ACGGA 1 cut(s) 1487
TspRI CASTG 3 cut(s) 259, 405, 460
Van91I CCANNNNNTGG 1 cut(s) 400
VpaK11BI GGWCC 1 cut(s) 116
VspI ATTAAT 1 cut(s) 345
XagI CCTNNNNNAGG 1 cut(s) 631
XapI RAATTY 2 cut(s) 292, 1328
XceI RCATGY 1 cut(s) 503
XmaJI CCTAGG 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 879
XmnI GAANNNNTTC 2 cut(s) 310, 1493
XspI CTAG 2 cut(s) 411, 1344
ZrmI AGTACT 2 cut(s) 766, 928
Zsp2I ATGCAT 2 cut(s) 1072, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.