Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002721

Physical Location & Seq

Genomic Coordinates Reverse (-)

4185492 .. 4189931

Gene Structure 4440 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1044300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGCATACCTTTCAATCACTCACTTCGTCATCTCACTCTCTCCTTCCCCCAATTCCTCGAAAACCTAATAATCCCAATCTTCGATCCCCAATCGCTTCGAATCGGACTTTTGCTCTCAAGTTTCCAGTGCAGGTTTGCGTTGGGTATAGGATAGTCATGGATCGGCTCGAAGCTCCTGCCCGGCTAATGATAGTTTCAGATCTTGATCATACAATGGTTGATCATCACGACACTGAGAACCTTTCCCTGCTCAGATTCAATTCTTTGTGGGAAGCAAATTACCGTCACGATTCTCTGCTAGTTTTTTCAACTGGAAGGTCACCGACACTGTACAAAGAATTGAGGAAAGAGAAACCCATGTTAACTCCAGATATAACAATTATGTCTGTGGGAACCGAGATAACATATGGTAACACAATGGTTCCTGATGATGGCTGGGTTGAGGTTCTGAATAAAAAATGGGATAGGAACATAGTCAAGGAGGAAGCCAGCAAATTTTCTGAACTTAAACTTCAGGCAGAAACAGAGCAACGGCCACACAAGGTTAGCTTTTATGTTGAGAAAGCCAAGGCTCAGGAAGTGACAAAGGCTCTTTCAGAAGTTTTGGTAAAGCGTAGGTTGGATGTGAAGATAATCTATAGTGGAGGAATGGATTTGGATATATTACCACAAGGTGCTGGCAAAGGACAAGCTCTTGCATATTTGCTTAAGAAATTTAAGAGTGAGGGAAGGCCACCTGTTAACACTCTTGTTTGTGGTGACTCTGGAAATGATGCTGAGCTTTTTAGCATTCCAGAAGTATATGGTGTCATGGTTAGCAATGCCCAAGAAGAATTGTTGCATTGGCATGCTAAAAATGCTAAGGGTAACACTAGGATTATTCATGCAACAGAGAGATGTGCAGCTGGAATCATACAAGCCATTGGCCACTTTAAGCTAGGTCCGAGTTTGCCCCCAAGAGATATTGCAGATTTTTCTGACTATAAACTGGAGAATCCAAATCCTGGTCACGAACTAGTAAAGTTTTTCTTGTTCTATGAGAAATGGAGACGCGCAGAAGTTGAGAATTCAGAGATCTATTTGGCAAGTTTGAAAGCAGATTGCTCTCCATCCGGTACGTTTGTCCATCCTTCTGGAGTTGAGCACTCTCTTTCTGACAGCATAAATGCTCTGAGAAACTGCTACGGAGACAAACAGGGAAAACAATTTCGGGTTTGGGTGGATGGGGTATTAGCTACACATGTTGGTTCAAACACATGGCTAGTGAAGTTCGATAAGTGGGAATTATCTGGTGAAGAACGTTATGCAACAAAGGGAACAGCTGTTATAAGTTCAAAGGGTTCCGGTGTTTCAGTTGGTTTCACTTGGATTCGAGTGCACCAGACATGGTACAAAGGATACGAAGCAAAAGATGATTCAACGTGGTTCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0016740 GO:0016757

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

S6PP S6PP_C

Protein Analysis

478

Amino Acids

53.8

Weight (kDa)

8.11

Isoelectric Point (pI)

33.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S6PP	PF05116	61 - 312	6.2e-100	Sucrose-6F-phosphate phosphohydrolase
Hydrolase_3	PF08282	151 - 280	8.2e-12	haloacid dehalogenase-like hydrolase
S6PP_C	PF08472	335 - 464	4.4e-54	Sucrose-6-phosphate phosphohydrolase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51420 AT1G51420 AT1G51420 AT1G51420 AT2G35840 AT2G35840 AT2G35840 AT2G35840 AT3G52340 AT3G52340 AT3G52340 AT3G52340 AT3G52340 AT3G52340 AT3G52340
fragaria_vesca	FvH4_6g20950 FvH4_6g20950
malus_domestica	MD12G1045400.v1.1 MD14G1044300.v1.1
prunus_persica	Prupe.7G089300_v2.0.a1 Prupe.7G089300_v2.0.a1
pyrus_communis	pycom12g03920 pycom14g03750
rosa_chinensis	RchiOBHm_Chr3g0477361
rosa_laevigata	RLG00000023729
rosa_multiflora	Rmu_sc0006369.1_g000008
rosa_roxburghii	Rroxscaffold_6G00404250 Rroxscaffold_6G00404730 Rroxscaffold_7G00202730
rosa_rugosa	Rorug03G0159700 Rorug03G0159700 Rorug03G0159700 Rorug03G0159700 Rorug03G0159700
rosa_samantha	Rh3AG210200 Rh3BG243600 Rh3CG237500 Rh3DG237100
rosa_wichuraiana	Rw0G000300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1328
Acc36I	ACCTGC	1 cut(s)	121
AccB7I	CCANNNNNTGG	1 cut(s)	1004
AccII	CGCG	1 cut(s)	1053
AclI	AACGTT	1 cut(s)	1300
AclWI	GGATC	2 cut(s)	78, 168
AcoI	YGGCCR	2 cut(s)	533, 925
AcsI	RAATTY	3 cut(s)	494, 713, 1066
AcuI	CTGAAG	1 cut(s)	497
AdeI	CACNNNGTG	1 cut(s)	674
AfaI	GTAC	3 cut(s)	332, 1117, 1391
AfiI	CCNNNNNNNGG	2 cut(s)	431, 1004
AflII	CTTAAG	1 cut(s)	707
AflIII	ACRYGT	1 cut(s)	1240
AgsI	TTSAA	7 cut(s)	14, 259, 309, 1093, 1251, 1335, 1419
AhlI	ACTAGT	1 cut(s)	1015
AjnI	CCWGG	1 cut(s)	1003
AluBI	AGCT	8 cut(s)	173, 549, 692, 781, 905, 937, 1235, 1322
AluI	AGCT	8 cut(s)	173, 549, 692, 781, 905, 937, 1235, 1322
Alw21I	GWGCWC	2 cut(s)	1146, 1380
Alw26I	GTCTC	2 cut(s)	1042, 1182
Alw44I	GTGCAC	1 cut(s)	1376
AlwI	GGATC	2 cut(s)	78, 168
AoxI	GGCC	3 cut(s)	533, 731, 925
ApaLI	GTGCAC	1 cut(s)	1376
ApeKI	GCWGC	1 cut(s)	902
ApoI	RAATTY	3 cut(s)	494, 713, 1066
AspLEI	GCGC	1 cut(s)	1055
AspS9I	GGNCC	1 cut(s)	941
AsuC2I	CCSGG	1 cut(s)	181
AsuHPI	GGTGA	3 cut(s)	312, 770, 1304
AsuII	TTCGAA	1 cut(s)	98
AvaII	GGWCC	1 cut(s)	941
BaeGI	GKGCMC	1 cut(s)	1380
BaeI	ACNNNNGTAYC	2 cut(s)	1107, 1140
BalI	TGGCCA	1 cut(s)	927
Bbv12I	GWGCWC	2 cut(s)	1146, 1380
BbvI	GCAGC	1 cut(s)	914
BccI	CCATC	4 cut(s)	425, 1117, 1134, 1217
BceAI	ACGGC	1 cut(s)	548
BcgI	CGANNNNNNTGC	2 cut(s)	158, 192
BciT130I	CCWGG	1 cut(s)	1005
BciVI	GTATCC	1 cut(s)	1391
BclI	TGATCA	2 cut(s)	205, 220
BcnI	CCSGG	1 cut(s)	181
BcoDI	GTCTC	2 cut(s)	1042, 1182
BcuI	ACTAGT	1 cut(s)	1015
BfaI	CTAG	5 cut(s)	299, 873, 938, 1016, 1262
BfmI	CTRYAG	1 cut(s)	637
BfrI	CTTAAG	1 cut(s)	707
BfuAI	ACCTGC	1 cut(s)	121
BfuI	GTATCC	1 cut(s)	1391
BglII	AGATCT	2 cut(s)	199, 1074
BisI	GCNGC	1 cut(s)	903
BlpI	GCTNAGC	1 cut(s)	777
BlsI	GCNGC	1 cut(s)	904
Bme1390I	CCNGG	2 cut(s)	181, 1005
Bme18I	GGWCC	1 cut(s)	941
BmgT120I	GGNCC	1 cut(s)	941
BmiI	GGNNCC	3 cut(s)	394, 423, 1342
BmrFI	CCNGG	2 cut(s)	181, 1005
BmsI	GCATC	1 cut(s)	763
BpmI	CTGGAG	3 cut(s)	351, 1010, 1155
Bpu10I	CCTNAGC	2 cut(s)	573, 861
Bpu1102I	GCTNAGC	1 cut(s)	777
Bpu14I	TTCGAA	1 cut(s)	98
BpuEI	CTTGAG	1 cut(s)	101
BpuMI	CCSGG	1 cut(s)	181
BsaBI	GATNNNNATC	1 cut(s)	204
BsaJI	CCNNGG	1 cut(s)	567
BsaWI	WCCGGW	2 cut(s)	1112, 1343
Bsc4I	CCNNNNNNNGG	2 cut(s)	431, 1004
Bse1I	ACTGG	3 cut(s)	125, 316, 993
Bse3DI	GCAATG	1 cut(s)	826
Bse8I	GATNNNNATC	1 cut(s)	204
BseBI	CCWGG	1 cut(s)	1005
BseDI	CCNNGG	1 cut(s)	567
BseGI	GGATG	4 cut(s)	628, 1109, 1126, 1228
BseJI	GATNNNNATC	1 cut(s)	204
BseLI	CCNNNNNNNGG	2 cut(s)	431, 1004
BseMI	GCAATG	1 cut(s)	826
BseMII	CTCAG	5 cut(s)	225, 265, 587, 768, 1163
BseNI	ACTGG	3 cut(s)	125, 316, 993
BseSI	GKGCMC	1 cut(s)	1380
BseXI	GCAGC	1 cut(s)	914
BseYI	CCCAGC	1 cut(s)	435
BsgI	GTGCAG	2 cut(s)	149, 921
Bsh1236I	CGCG	1 cut(s)	1053
BshFI	GGCC	3 cut(s)	535, 733, 927
BsiHKAI	GWGCWC	2 cut(s)	1146, 1380
BsiSI	CCGG	3 cut(s)	181, 1113, 1344
BslI	CCNNNNNNNGG	2 cut(s)	431, 1004
BsmAI	GTCTC	2 cut(s)	1042, 1182
BsmBI	CGTCTC	1 cut(s)	1042
BsmI	GAATGC	1 cut(s)	789
BsnI	GGCC	3 cut(s)	535, 733, 927
Bsp119I	TTCGAA	1 cut(s)	98
Bsp1286I	GDGCHC	2 cut(s)	1146, 1380
Bsp1407I	TGTACA	1 cut(s)	330
Bsp143I	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
Bsp1720I	GCTNAGC	1 cut(s)	777
BspANI	GGCC	3 cut(s)	535, 733, 927
BspCNI	CTCAG	5 cut(s)	226, 264, 586, 769, 1164
BspFNI	CGCG	1 cut(s)	1053
BspLI	GGNNCC	3 cut(s)	394, 423, 1342
BspMI	ACCTGC	1 cut(s)	121
BspPI	GGATC	2 cut(s)	78, 168
BspT104I	TTCGAA	1 cut(s)	98
BspTI	CTTAAG	1 cut(s)	707
BsrDI	GCAATG	1 cut(s)	826
BsrGI	TGTACA	1 cut(s)	330
BsrI	ACTGG	3 cut(s)	125, 316, 993
BssECI	CCNNGG	1 cut(s)	567
BssMI	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
BssT1I	CCWWGG	1 cut(s)	567
Bst2UI	CCWGG	1 cut(s)	1005
Bst4CI	ACNGT	2 cut(s)	284, 330
BstAFI	CTTAAG	1 cut(s)	707
BstAUI	TGTACA	1 cut(s)	330
BstBI	TTCGAA	1 cut(s)	98
BstC8I	GCNNGC	3 cut(s)	490, 679, 849
BstDEI	CTNAG	6 cut(s)	234, 251, 573, 777, 861, 1172
BstEII	GGTNACC	1 cut(s)	318
BstF5I	GGATG	4 cut(s)	628, 1109, 1126, 1228
BstFNI	CGCG	1 cut(s)	1053
BstHHI	GCGC	1 cut(s)	1055
BstKTI	GATC	6 cut(s)	86, 163, 202, 208, 223, 1077
BstMAI	GTCTC	2 cut(s)	1042, 1182
BstMBI	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
BstMWI	GCNNNNNNNGC	1 cut(s)	857
BstNI	CCWGG	1 cut(s)	1005
BstNSI	RCATGY	2 cut(s)	851, 1244
BstPI	GGTNACC	1 cut(s)	318
BstSCI	CCNGG	2 cut(s)	179, 1003
BstSFI	CTRYAG	1 cut(s)	637
BstSLI	GKGCMC	1 cut(s)	1380
BstUI	CGCG	1 cut(s)	1053
BstV1I	GCAGC	1 cut(s)	914
BstX2I	RGATCY	2 cut(s)	199, 1074
BstXI	CCANNNNNNTGG	1 cut(s)	1133
BstYI	RGATCY	2 cut(s)	199, 1074
BsuI	GTATCC	1 cut(s)	1391
BsuRI	GGCC	3 cut(s)	535, 733, 927
BtsCI	GGATG	4 cut(s)	628, 1109, 1126, 1228
BtsIMutI	CAGTG	3 cut(s)	132, 231, 326
BveI	ACCTGC	1 cut(s)	121
Cac8I	GCNNGC	3 cut(s)	490, 679, 849
CfoI	GCGC	1 cut(s)	1055
Cfr13I	GGNCC	1 cut(s)	941
CseI	GACGC	1 cut(s)	1059
Csp6I	GTAC	3 cut(s)	331, 1116, 1390
CviAII	CATG	8 cut(s)	157, 358, 811, 848, 884, 1241, 1257, 1386
CviQI	GTAC	3 cut(s)	331, 1116, 1390
DdeI	CTNAG	6 cut(s)	234, 251, 573, 777, 861, 1172
DpnI	GATC	6 cut(s)	85, 162, 201, 207, 222, 1076
DpnII	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
DraIII	CACNNNGTG	1 cut(s)	674
EaeI	YGGCCR	2 cut(s)	533, 925
Eco130I	CCWWGG	1 cut(s)	567
Eco47I	GGWCC	1 cut(s)	941
Eco57I	CTGAAG	1 cut(s)	497
Eco91I	GGTNACC	1 cut(s)	318
EcoO65I	GGTNACC	1 cut(s)	318
EcoRI	GAATTC	1 cut(s)	1066
EcoRII	CCWGG	1 cut(s)	1003
EcoT14I	CCWWGG	1 cut(s)	567
EcoT22I	ATGCAT	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	567
Esp3I	CGTCTC	1 cut(s)	1042
FaeI	CATG	8 cut(s)	160, 361, 814, 851, 887, 1244, 1260, 1389
FalI	AAGNNNNNCTT	4 cut(s)	533, 565, 1013, 1045
FatI	CATG	8 cut(s)	156, 357, 810, 847, 883, 1240, 1256, 1385
FauNDI	CATATG	1 cut(s)	406
FbaI	TGATCA	2 cut(s)	205, 220
Fnu4HI	GCNGC	1 cut(s)	903
FokI	GGATG	4 cut(s)	635, 1096, 1113, 1235
Fsp4HI	GCNGC	1 cut(s)	903
FspBI	CTAG	5 cut(s)	299, 873, 938, 1016, 1262
GlaI	GCGC	1 cut(s)	1054
GluI	GCNGC	1 cut(s)	903
GsaI	CCCAGC	1 cut(s)	439
GsuI	CTGGAG	3 cut(s)	351, 1010, 1155
HaeIII	GGCC	3 cut(s)	535, 733, 927
HapII	CCGG	3 cut(s)	181, 1113, 1344
HgaI	GACGC	1 cut(s)	1059
HhaI	GCGC	1 cut(s)	1055
Hin1II	CATG	8 cut(s)	160, 361, 814, 851, 887, 1244, 1260, 1389
Hin6I	GCGC	1 cut(s)	1053
HinP1I	GCGC	1 cut(s)	1053
HincII	GTYRAC	2 cut(s)	363, 742
HindII	GTYRAC	2 cut(s)	363, 742
HinfI	GANTC	8 cut(s)	100, 255, 290, 761, 909, 994, 1369, 1415
HpaI	GTTAAC	2 cut(s)	363, 742
HpaII	CCGG	3 cut(s)	181, 1113, 1344
HphI	GGTGA	3 cut(s)	312, 770, 1304
Hpy166II	GTNNAC	3 cut(s)	363, 742, 1378
Hpy8I	GTNNAC	3 cut(s)	363, 742, 1378
HpyAV	CCTTC	4 cut(s)	53, 309, 723, 1140
HpyCH4III	ACNGT	2 cut(s)	284, 330
HpyCH4IV	ACGT	3 cut(s)	1118, 1300, 1421
HpyCH4V	TGCA	9 cut(s)	4, 130, 698, 841, 887, 902, 968, 1307, 1378
HpyF10VI	GCNNNNNNNGC	1 cut(s)	857
HpyF3I	CTNAG	6 cut(s)	234, 251, 573, 777, 861, 1172
HpySE526I	ACGT	3 cut(s)	1118, 1300, 1421
Hsp92II	CATG	8 cut(s)	160, 361, 814, 851, 887, 1244, 1260, 1389
HspAI	GCGC	1 cut(s)	1053
Ksp22I	TGATCA	2 cut(s)	205, 220
KspAI	GTTAAC	2 cut(s)	363, 742
Kzo9I	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
LmnI	GCTCC	1 cut(s)	178
Lsp1109I	GCAGC	1 cut(s)	914
LweI	GCATC	1 cut(s)	763
MaeI	CTAG	5 cut(s)	299, 873, 938, 1016, 1262
MaeII	ACGT	3 cut(s)	1118, 1300, 1421
MaeIII	GTNAC	7 cut(s)	284, 318, 410, 580, 758, 866, 1007
MalI	GATC	6 cut(s)	85, 162, 201, 207, 222, 1076
MboI	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
MboII	GAAGA	5 cut(s)	71, 640, 842, 1307, 1420
MflI	RGATCY	2 cut(s)	199, 1074
MhlI	GDGCHC	2 cut(s)	1146, 1380
MlsI	TGGCCA	1 cut(s)	927
MluNI	TGGCCA	1 cut(s)	927
MlyI	GAGTC	1 cut(s)	755
MmeI	TCCRAC	1 cut(s)	600
MnlI	CCTC	6 cut(s)	66, 336, 436, 475, 638, 718
Mox20I	TGGCCA	1 cut(s)	927
Mph1103I	ATGCAT	1 cut(s)	6
MscI	TGGCCA	1 cut(s)	927
MseI	TTAA	6 cut(s)	362, 507, 708, 717, 741, 933
MslI	CAYNNNNRTG	1 cut(s)	846
Msp20I	TGGCCA	1 cut(s)	927
MspA1I	CMGCKG	2 cut(s)	905, 1322
MspCI	CTTAAG	1 cut(s)	707
MspI	CCGG	3 cut(s)	181, 1113, 1344
MspR9I	CCNGG	2 cut(s)	181, 1005
Mva1269I	GAATGC	1 cut(s)	789
MvaI	CCWGG	1 cut(s)	1005
MvnI	CGCG	1 cut(s)	1053
MwoI	GCNNNNNNNGC	1 cut(s)	857
NciI	CCSGG	1 cut(s)	181
NdeI	CATATG	1 cut(s)	406
NdeII	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
NlaIII	CATG	8 cut(s)	160, 361, 814, 851, 887, 1244, 1260, 1389
NlaIV	GGNNCC	3 cut(s)	394, 423, 1342
NmuCI	GTSAC	5 cut(s)	284, 318, 580, 758, 1007
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	851, 1244
NspV	TTCGAA	1 cut(s)	98
PaeI	GCATGC	1 cut(s)	851
PciI	ACATGT	1 cut(s)	1240
PctI	GAATGC	1 cut(s)	789
PfeI	GAWTC	7 cut(s)	100, 255, 290, 909, 994, 1369, 1415
PflMI	CCANNNNNTGG	1 cut(s)	1004
PkrI	GCNGC	1 cut(s)	904
PleI	GAGTC	1 cut(s)	755
PpsI	GAGTC	1 cut(s)	755
PscI	ACATGT	1 cut(s)	1240
PsiI	TTATAA	1 cut(s)	1328
Psp1406I	AACGTT	1 cut(s)	1300
Psp6I	CCWGG	1 cut(s)	1003
PspEI	GGTNACC	1 cut(s)	318
PspFI	CCCAGC	1 cut(s)	435
PspGI	CCWGG	1 cut(s)	1003
PspN4I	GGNNCC	3 cut(s)	394, 423, 1342
PspPI	GGNCC	1 cut(s)	941
PsuI	RGATCY	2 cut(s)	199, 1074
PvuII	CAGCTG	2 cut(s)	905, 1322
RsaI	GTAC	3 cut(s)	332, 1117, 1391
RsaNI	GTAC	3 cut(s)	331, 1116, 1390
RseI	CAYNNNNRTG	1 cut(s)	846
SaqAI	TTAA	6 cut(s)	362, 507, 708, 717, 741, 933
SatI	GCNGC	1 cut(s)	903
Sau3AI	GATC	6 cut(s)	83, 160, 199, 205, 220, 1074
Sau96I	GGNCC	1 cut(s)	941
SchI	GAGTC	1 cut(s)	755
ScrFI	CCNGG	2 cut(s)	181, 1005
SduI	GDGCHC	2 cut(s)	1146, 1380
SfaNI	GCATC	1 cut(s)	763
SfcI	CTRYAG	1 cut(s)	637
SfuI	TTCGAA	1 cut(s)	98
SinI	GGWCC	1 cut(s)	941
SmiMI	CAYNNNNRTG	1 cut(s)	846
SmlI	CTYRAG	2 cut(s)	116, 707
SmoI	CTYRAG	2 cut(s)	116, 707
SpeI	ACTAGT	1 cut(s)	1015
SphI	GCATGC	1 cut(s)	851
SspMI	CTAG	5 cut(s)	299, 873, 938, 1016, 1262
StyD4I	CCNGG	2 cut(s)	179, 1003
StyI	CCWWGG	1 cut(s)	567
TaaI	ACNGT	2 cut(s)	284, 330
TaiI	ACGT	3 cut(s)	1121, 1303, 1424
TaqI	TCGA	6 cut(s)	58, 82, 98, 168, 1272, 1372
TatI	WGTACW	1 cut(s)	330
TfiI	GAWTC	7 cut(s)	100, 255, 290, 909, 994, 1369, 1415
Tru1I	TTAA	6 cut(s)	362, 507, 708, 717, 741, 933
Tru9I	TTAA	6 cut(s)	362, 507, 708, 717, 741, 933
TscAI	CASTG	3 cut(s)	132, 238, 333
TseFI	GTSAC	5 cut(s)	284, 318, 580, 758, 1007
TseI	GCWGC	1 cut(s)	902
Tsp45I	GTSAC	5 cut(s)	284, 318, 580, 758, 1007
TspDTI	ATGAA	1 cut(s)	872
TspGWI	ACGGA	1 cut(s)	1200
TspRI	CASTG	3 cut(s)	132, 238, 333
Van91I	CCANNNNNTGG	1 cut(s)	1004
Vha464I	CTTAAG	1 cut(s)	707
VneI	GTGCAC	1 cut(s)	1376
VpaK11BI	GGWCC	1 cut(s)	941
XapI	RAATTY	3 cut(s)	494, 713, 1066
XceI	RCATGY	2 cut(s)	851, 1244
XspI	CTAG	5 cut(s)	299, 873, 938, 1016, 1262
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.