Rorug03G0159700

Prolycopene isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002721
Physical Location & Seq
Reverse (-)
13241796 .. 13248901
7106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0159700.1

Sequence Viewer

Length: 1653 bp
ATGGGGCACAGACATCCGTTCAGCGCATCTCAAATTTTTGAGAGTGAAAATGATCCGAATTGGAACCATATGAACACAGAACACCCATTTGGACATCTGGCTAGGGCTGGGGCGGGAGAGAATGGTTCTTTGTTTTATCCAGTGGAAAATATGTTCAATGATGGAATGCATTTTCCTTCGAATTGGAACCCGCCTATGAGGTCATATGGGTATTCTCCCTTGAGCCACAGTGTTGATATGCCACCTCAATATCAACCCGATGCTTCTGGTCCATCTCATGATCCTTTTCAACATCCACCAAGTGCTAGTACTTTTGGTATGGCCAGTGGAAACTATGCTCACCATCCATCTTCGTCCAGCTATGACCGTCCAGCATTCCATGGTGTTGAGGGTGGTTTTGTTGATCTCACAATGAGCAATGGAAGAGGGCCACACAAGCGAAAAAGTCCTGGGATCCCTTCTGTAGCGGAGAGAGGCAGCACGAGCAGATACTACGGCGCTGGAAGTTCCTCTGACCTCTCTAGATCTACAGAATTACAGCAGGAGAAAGTAAACATAGATACCCCACACATGCCGTGGGATCACATGACTATGATTCACCCCAGCTATAGAGGAAATGACCCTACAAGTAGGGGTGAGGGATCTATGAGAAATGTGAGAAGCCGGTCAGAACATGATTTGGAGTCCAACCTAGCAAGGACACATCTATCGAACAATCCTATACATAGTTCGTATTCTGCAAATTTCCCTGTTGACCACCCTAGTACGATTGATCTTTCGGGTCAGAGTTCCAATGTTTTAGCCCCTGAATGGAACCATATTAGCGTATCGCCTCCTCATGGAAGGATTTTACCTTCAGATACAAGTGGTTTTAGTCATGATCCCAACCACTTCCTTGTAGGCAGCAGTATGGTTAATGCTTCTGCCGAAGCTGGTGTATACCACCATGATTTCATTCCAACCAGGAGTGCTGCTGTTCCTCCAAGTTATCCTGGTGCCTTACCCCATACTGTAAGGGGAGTTCGCAGCAACTATTCTCAAAGATCTAGCCCAACCTTCAGGGCTTCTTCAAGCAGCATGAGGTTTGGGCCTGTGACACATCCAGATGAAGGATTGCAGTTGGTATCTGAGAGTTACCCAAGACATCCTAGGCCAGTGGCTACAGTTGGGTGGCGTAACAGCGACAGGAATGGTAGATCAAGGATGTCTAATGATAGATATCGAGTTTCTGAAGAGACTAGTCTCCATGATCGGTTTTCATCTGAGGGTTTTATGATTGCTGATCGGCCAGCTTTCTATGGTTCCAGAAGTATGCTTGATCATCATAGAGACTTGAGACTAGACATAGACAACATGAGTTACGAGGAACTCCTTGCATTGGGGGAAAGGATTGGGCATGTCAACACAGGTGTATCTGAAGATCTAATACCGAAGTGTTTGACAGAAACAATATATTGTTCTTCAGACCTAAACCAGGAGGAAGGCACATGTGTAATCTGCCTGGAAGAGTACGAGGATAGGGATGATGTTGCAGCACTGAAAACGTGCGGCCATGATTACCATGTGACTTGCATCAAAAAGTGGTTGTCAATGAAAAATTCTTGTCCAATCTGCAAAGGTTCTGCCCTTGCCGATAATACTAAGGGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0008152 GO:0016491 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
Amino_oxidase

Protein Analysis

550

Amino Acids

60.9

Weight (kDa)

6.19

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 486 - 539 3.6e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 496 - 539 6.6e-14 Ring finger domain
zf-C3HC4 PF00097 497 - 538 2.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 497 - 538 4.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 497 - 525 2.4e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 995
AccI GTMKAC 1 cut(s) 939
AciI CCGC 4 cut(s) 113, 191, 467, 1548
AclWI GGATC 7 cut(s) 47, 275, 448, 461, 588, 649, 875
AcoI YGGCCR 3 cut(s) 321, 1286, 1549
AcsI RAATTY 3 cut(s) 33, 742, 1597
AcuI CTGAAG 5 cut(s) 840, 1042, 1251, 1437, 1446
AdeI CACNNNGTG 1 cut(s) 302
AfaI GTAC 3 cut(s) 310, 766, 1511
AfiI CCNNNNNNNGG 5 cut(s) 810, 839, 1109, 1378, 1474
AflIII ACRYGT 1 cut(s) 1487
AgsI TTSAA 3 cut(s) 157, 290, 1071
AhlI ACTAGT 1 cut(s) 1238
AjnI CCWGG 5 cut(s) 448, 962, 991, 1473, 1500
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 360, 606, 932, 1292
AluI AGCT 4 cut(s) 360, 606, 932, 1292
Alw26I GTCTC 4 cut(s) 1229, 1247, 1323, 1330
AlwI GGATC 7 cut(s) 47, 275, 448, 461, 588, 649, 875
AoxI GGCC 6 cut(s) 321, 428, 1088, 1151, 1286, 1549
ApeKI GCWGC 6 cut(s) 477, 903, 971, 1026, 1074, 1532
ApoI RAATTY 3 cut(s) 33, 742, 1597
AspA2I CCTAGG 1 cut(s) 1148
AspLEI GCGC 2 cut(s) 26, 500
AspS9I GGNCC 3 cut(s) 269, 428, 1088
AsuHPI GGTGA 3 cut(s) 332, 590, 647
AsuII TTCGAA 1 cut(s) 179
AvaII GGWCC 1 cut(s) 269
AvrII CCTAGG 1 cut(s) 1148
BaeGI GKGCMC 1 cut(s) 9
BaeI ACNNNNGTAYC 2 cut(s) 1395, 1428
BalI TGGCCA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 453
BanI GGYRCC 1 cut(s) 995
BarI GAAGNNNNNNTAC 2 cut(s) 1410, 1442
BauI CACGAG 1 cut(s) 481
BbvI GCAGC 6 cut(s) 489, 915, 958, 1038, 1086, 1544
BccI CCATC 4 cut(s) 155, 280, 351, 355
BceAI ACGGC 2 cut(s) 511, 559
BciT130I CCWGG 5 cut(s) 450, 964, 993, 1475, 1502
BclI TGATCA 1 cut(s) 1318
BcoDI GTCTC 4 cut(s) 1229, 1247, 1323, 1330
BcuI ACTAGT 1 cut(s) 1238
BfaI CTAG 9 cut(s) 102, 306, 522, 692, 762, 1047, 1149, 1239, 1340
BfmI CTRYAG 4 cut(s) 462, 528, 607, 1161
BfoI RGCGCY 1 cut(s) 501
BglII AGATCT 3 cut(s) 524, 1043, 1420
BisI GCNGC 7 cut(s) 478, 904, 972, 1027, 1075, 1533, 1549
BlnI CCTAGG 1 cut(s) 1148
BlsI GCNGC 7 cut(s) 479, 905, 973, 1028, 1076, 1534, 1550
BmcAI AGTACT 1 cut(s) 310
Bme1390I CCNGG 5 cut(s) 450, 964, 993, 1475, 1502
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 3 cut(s) 269, 428, 1088
BmiI GGNNCC 6 cut(s) 65, 188, 455, 815, 997, 1303
BmrFI CCNGG 5 cut(s) 450, 964, 993, 1475, 1502
BmsI GCATC 3 cut(s) 35, 250, 1581
Bpu14I TTCGAA 1 cut(s) 179
BpuEI CTTGAG 2 cut(s) 241, 1354
BsaBI GATNNNNATC 1 cut(s) 1218
BsaJI CCNNGG 4 cut(s) 379, 449, 575, 1148
Bsc4I CCNNNNNNNGG 5 cut(s) 810, 839, 1109, 1378, 1474
Bse118I RCCGGY 1 cut(s) 663
Bse1I ACTGG 3 cut(s) 140, 324, 1154
Bse3DI GCAATG 1 cut(s) 424
Bse8I GATNNNNATC 1 cut(s) 1218
BseBI CCWGG 5 cut(s) 450, 964, 993, 1475, 1502
BseDI CCNNGG 4 cut(s) 379, 449, 575, 1148
BseGI GGATG 7 cut(s) 13, 292, 343, 1099, 1144, 1209, 1528
BseJI GATNNNNATC 1 cut(s) 1218
BseLI CCNNNNNNNGG 5 cut(s) 810, 839, 1109, 1378, 1474
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 2 cut(s) 1119, 1254
BseNI ACTGG 3 cut(s) 140, 324, 1154
BseRI GAGGAG 1 cut(s) 825
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 6 cut(s) 489, 915, 958, 1038, 1086, 1544
BseYI CCCAGC 2 cut(s) 107, 602
BshFI GGCC 6 cut(s) 323, 430, 1090, 1153, 1288, 1551
BshNI GGYRCC 1 cut(s) 995
BsiSI CCGG 1 cut(s) 664
BslI CCNNNNNNNGG 5 cut(s) 810, 839, 1109, 1378, 1474
BsmAI GTCTC 4 cut(s) 1229, 1247, 1323, 1330
BsmI GAATGC 2 cut(s) 171, 374
BsnI GGCC 6 cut(s) 323, 430, 1090, 1153, 1288, 1551
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 4 cut(s) 113, 191, 467, 1548
BspANI GGCC 6 cut(s) 323, 430, 1090, 1153, 1288, 1551
BspCNI CTCAG 2 cut(s) 1120, 1255
BspHI TCATGA 2 cut(s) 277, 877
BspLI GGNNCC 6 cut(s) 65, 188, 455, 815, 997, 1303
BspPI GGATC 7 cut(s) 47, 275, 448, 461, 588, 649, 875
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 424
BsrFI RCCGGY 1 cut(s) 663
BsrI ACTGG 3 cut(s) 140, 324, 1154
BssAI RCCGGY 1 cut(s) 663
BssECI CCNNGG 4 cut(s) 379, 449, 575, 1148
BssNAI GTATAC 1 cut(s) 940
BssSI CACGAG 1 cut(s) 481
BssT1I CCWWGG 2 cut(s) 379, 1148
Bst1107I GTATAC 1 cut(s) 940
Bst2BI CACGAG 1 cut(s) 481
Bst2UI CCWGG 5 cut(s) 450, 964, 993, 1475, 1502
Bst4CI ACNGT 4 cut(s) 230, 368, 1012, 1165
Bst6I CTCTTC 3 cut(s) 418, 1227, 1500
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 1290
BstDEI CTNAG 3 cut(s) 1128, 1263, 1641
BstDSI CCRYGG 2 cut(s) 379, 575
BstENI CCTNNNNNAGG 1 cut(s) 1472
BstF5I GGATG 7 cut(s) 13, 292, 343, 1099, 1144, 1209, 1528
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 2 cut(s) 26, 500
BstMAI GTCTC 4 cut(s) 1229, 1247, 1323, 1330
BstMWI GCNNNNNNNGC 2 cut(s) 436, 483
BstNI CCWGG 5 cut(s) 450, 964, 993, 1475, 1502
BstNSI RCATGY 3 cut(s) 574, 1400, 1491
BstSCI CCNGG 5 cut(s) 448, 962, 991, 1473, 1500
BstSFI CTRYAG 4 cut(s) 462, 528, 607, 1161
BstSLI GKGCMC 1 cut(s) 9
BstV1I GCAGC 6 cut(s) 489, 915, 958, 1038, 1086, 1544
BstX2I RGATCY 5 cut(s) 453, 524, 641, 1043, 1420
BstYI RGATCY 5 cut(s) 453, 524, 641, 1043, 1420
BstZ17I GTATAC 1 cut(s) 940
BsuRI GGCC 6 cut(s) 323, 430, 1090, 1153, 1288, 1551
BtgI CCRYGG 2 cut(s) 379, 575
BtsCI GGATG 7 cut(s) 13, 292, 343, 1099, 1144, 1209, 1528
BtsIMutI CAGTG 5 cut(s) 147, 235, 331, 1161, 1535
Cac8I GCNNGC 1 cut(s) 1290
CciI TCATGA 2 cut(s) 277, 877
CfoI GCGC 2 cut(s) 26, 500
Cfr10I RCCGGY 1 cut(s) 663
Cfr13I GGNCC 3 cut(s) 269, 428, 1088
Csp6I GTAC 3 cut(s) 309, 765, 1510
CviQI GTAC 3 cut(s) 309, 765, 1510
DdeI CTNAG 3 cut(s) 1128, 1263, 1641
DraIII CACNNNGTG 1 cut(s) 302
EaeI YGGCCR 3 cut(s) 321, 1286, 1549
Eam1104I CTCTTC 3 cut(s) 418, 1227, 1500
EarI CTCTTC 3 cut(s) 418, 1227, 1500
Eco130I CCWWGG 2 cut(s) 379, 1148
Eco32I GATATC 1 cut(s) 1220
Eco47I GGWCC 1 cut(s) 269
Eco57I CTGAAG 5 cut(s) 840, 1042, 1251, 1437, 1446
EcoNI CCTNNNNNAGG 1 cut(s) 1472
EcoRII CCWGG 5 cut(s) 448, 962, 991, 1473, 1500
EcoRV GATATC 1 cut(s) 1220
EcoT14I CCWWGG 2 cut(s) 379, 1148
EcoT22I ATGCAT 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 379, 1148
FauI CCCGC 2 cut(s) 106, 198
FauNDI CATATG 2 cut(s) 69, 205
FbaI TGATCA 1 cut(s) 1318
FblI GTMKAC 1 cut(s) 939
Fnu4HI GCNGC 7 cut(s) 478, 904, 972, 1027, 1075, 1533, 1549
FokI GGATG 6 cut(s) 279, 330, 1086, 1131, 1216, 1535
Fsp4HI GCNGC 7 cut(s) 478, 904, 972, 1027, 1075, 1533, 1549
FspBI CTAG 9 cut(s) 102, 306, 522, 692, 762, 1047, 1149, 1239, 1340
GlaI GCGC 2 cut(s) 25, 499
GluI GCNGC 7 cut(s) 478, 904, 972, 1027, 1075, 1533, 1549
GsaI CCCAGC 2 cut(s) 111, 606
HaeII RGCGCY 1 cut(s) 501
HaeIII GGCC 6 cut(s) 323, 430, 1090, 1153, 1288, 1551
HapII CCGG 1 cut(s) 664
HhaI GCGC 2 cut(s) 26, 500
Hin6I GCGC 2 cut(s) 24, 498
HinP1I GCGC 2 cut(s) 24, 498
HincII GTYRAC 2 cut(s) 754, 1402
HindII GTYRAC 2 cut(s) 754, 1402
HinfI GANTC 2 cut(s) 595, 683
HpaII CCGG 1 cut(s) 664
HphI GGTGA 3 cut(s) 332, 590, 647
Hpy166II GTNNAC 4 cut(s) 553, 754, 940, 1402
Hpy188III TCNNGA 5 cut(s) 278, 522, 878, 1103, 1305
Hpy8I GTNNAC 4 cut(s) 553, 754, 940, 1402
HpyAV CCTTC 7 cut(s) 186, 468, 837, 864, 1066, 1103, 1475
HpyCH4III ACNGT 4 cut(s) 230, 368, 1012, 1165
HpyCH4IV ACGT 1 cut(s) 1544
HpyCH4V TGCA 7 cut(s) 169, 740, 1117, 1376, 1532, 1572, 1614
HpyF10VI GCNNNNNNNGC 2 cut(s) 436, 483
HpyF3I CTNAG 3 cut(s) 1128, 1263, 1641
HpySE526I ACGT 1 cut(s) 1544
HspAI GCGC 2 cut(s) 24, 498
Ksp22I TGATCA 1 cut(s) 1318
Lsp1109I GCAGC 6 cut(s) 489, 915, 958, 1038, 1086, 1544
LweI GCATC 3 cut(s) 35, 250, 1581
MaeI CTAG 9 cut(s) 102, 306, 522, 692, 762, 1047, 1149, 1239, 1340
MaeII ACGT 1 cut(s) 1544
MaeIII GTNAC 5 cut(s) 1093, 1133, 1175, 1358, 1564
MboII GAAGA 7 cut(s) 342, 435, 1059, 1244, 1430, 1452, 1517
MflI RGATCY 5 cut(s) 453, 524, 641, 1043, 1420
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 6 cut(s) 33, 58, 181, 533, 742, 1597
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 2 cut(s) 711, 983
Mox20I TGGCCA 1 cut(s) 323
Mph1103I ATGCAT 1 cut(s) 171
MscI TGGCCA 1 cut(s) 323
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 2 cut(s) 590, 1104
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 1 cut(s) 664
MspR9I CCNGG 5 cut(s) 450, 964, 993, 1475, 1502
Mva1269I GAATGC 2 cut(s) 171, 374
MvaI CCWGG 5 cut(s) 450, 964, 993, 1475, 1502
MwoI GCNNNNNNNGC 2 cut(s) 436, 483
NcoI CCATGG 1 cut(s) 379
NdeI CATATG 2 cut(s) 69, 205
NlaIV GGNNCC 6 cut(s) 65, 188, 455, 815, 997, 1303
NmuCI GTSAC 2 cut(s) 1093, 1564
NsiI ATGCAT 1 cut(s) 171
NspI RCATGY 3 cut(s) 574, 1400, 1491
NspV TTCGAA 1 cut(s) 179
PagI TCATGA 2 cut(s) 277, 877
PciI ACATGT 1 cut(s) 1487
PctI GAATGC 2 cut(s) 171, 374
PfeI GAWTC 1 cut(s) 595
PkrI GCNGC 7 cut(s) 479, 905, 973, 1028, 1076, 1534, 1550
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PscI ACATGT 1 cut(s) 1487
Psp6I CCWGG 5 cut(s) 448, 962, 991, 1473, 1500
PspFI CCCAGC 2 cut(s) 107, 602
PspGI CCWGG 5 cut(s) 448, 962, 991, 1473, 1500
PspN4I GGNNCC 6 cut(s) 65, 188, 455, 815, 997, 1303
PspPI GGNCC 3 cut(s) 269, 428, 1088
PsrI GAACNNNNNNTAC 2 cut(s) 1005, 1037
PsuI RGATCY 5 cut(s) 453, 524, 641, 1043, 1420
RsaI GTAC 3 cut(s) 310, 766, 1511
RsaNI GTAC 3 cut(s) 309, 765, 1510
RseI CAYNNNNRTG 2 cut(s) 590, 1104
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 7 cut(s) 478, 904, 972, 1027, 1075, 1533, 1549
Sau96I GGNCC 3 cut(s) 269, 428, 1088
ScaI AGTACT 1 cut(s) 310
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 5 cut(s) 450, 964, 993, 1475, 1502
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 3 cut(s) 35, 250, 1581
SfcI CTRYAG 4 cut(s) 462, 528, 607, 1161
SfuI TTCGAA 1 cut(s) 179
SinI GGWCC 1 cut(s) 269
SmiMI CAYNNNNRTG 2 cut(s) 590, 1104
SmlI CTYRAG 2 cut(s) 220, 1333
SmoI CTYRAG 2 cut(s) 220, 1333
SpeI ACTAGT 1 cut(s) 1238
Sse9I AATT 6 cut(s) 33, 58, 181, 533, 742, 1597
SsiI CCGC 4 cut(s) 113, 191, 467, 1548
SspMI CTAG 9 cut(s) 102, 306, 522, 692, 762, 1047, 1149, 1239, 1340
StyD4I CCNGG 5 cut(s) 448, 962, 991, 1473, 1500
StyI CCWWGG 2 cut(s) 379, 1148
TaaI ACNGT 4 cut(s) 230, 368, 1012, 1165
TaiI ACGT 1 cut(s) 1547
TaqI TCGA 3 cut(s) 179, 710, 1222
TasI AATT 6 cut(s) 33, 58, 181, 533, 742, 1597
TatI WGTACW 1 cut(s) 308
TauI GCSGC 1 cut(s) 1551
TfiI GAWTC 1 cut(s) 595
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TscAI CASTG 5 cut(s) 147, 235, 331, 1161, 1542
TseFI GTSAC 2 cut(s) 1093, 1564
TseI GCWGC 6 cut(s) 477, 903, 971, 1026, 1074, 1532
Tsp45I GTSAC 2 cut(s) 1093, 1564
TspDTI ATGAA 5 cut(s) 86, 943, 1122, 1248, 1607
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 5 cut(s) 147, 235, 331, 1161, 1542
VpaK11BI GGWCC 1 cut(s) 269
XagI CCTNNNNNAGG 1 cut(s) 1472
XapI RAATTY 3 cut(s) 33, 742, 1597
XbaI TCTAGA 1 cut(s) 521
XceI RCATGY 3 cut(s) 574, 1400, 1491
XcmI CCANNNNNNNNNTGG 1 cut(s) 573
XmaJI CCTAGG 1 cut(s) 1148
XmiI GTMKAC 1 cut(s) 939
XspI CTAG 9 cut(s) 102, 306, 522, 692, 762, 1047, 1149, 1239, 1340
ZrmI AGTACT 1 cut(s) 310
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.