MD14G1055300.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009232
Physical Location & Seq
Reverse (-)
5646987 .. 5652615
5629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1055300.v1.1.491

Sequence Viewer

Length: 3801 bp
ATGCGTGGAGCATGGAAAACGTGGACGGCAAGAGAGATAAGCATGAAGAGAGACTTAATCCATGACATTCTATGGGTGTATCTGAATAAACAATATGACAGCAGCTCTCTACTTAAGAACATTGCCGGCCAGCTATCTCTAATCTCTATTCCTGAGGAATATGATGACGATGACGTCAAGGTGGAGGAGGAGAACATGAGGTTGGAATTGAAACAGAGAATAGCCATCAAACTTAAAGGATTGATATCCGCAGCACATAAAGAAGACAAATATTTTCTACTAATCCTGGATGATGTTCCTTACGAGCGGTCTGTGAAAGACATTATCTCAGAACTGAAGACGCTGCCGGGTTTGAATGATATGAGTTCTTTTAAGGTTTTAATAACTAGAGATGATGGTGACAAAAAGCGCAACGCAGAGGAGAAGCGCACCACAGAGGAGGAGCACGGAGCAATTCAGACCATCCACACAAACGTAGATCAATTGTCTACAGAAGAGTCAAAGCTTTTATTGAAAGAAAATATCAAGAAAGTATTTTCCGACTCTCCGGGCTTTGAAATGTTATCCAAGGTGATTGTGGAGATGAGAAAGTCCATGCCTAGTGAAATCATCGCAATGGCAAAAGCATTCAACTCTGTTGAACATATTGGTTCTGGGGTTTGGTCATTGGAAAGTGTTTCGGAAGCAGCAGCTGATGATGAGAGAGCTGCAATAAATCTGCTACATCGTGCTTGGTATGACACGTTGCCAAGCGGTGTTCTGGCAGACTGTTTTTGGCATAGCATGCAATTTTTCAGCAATAATGTCTGTCTGCATTACAAAGAGTTGATCACTCATTGGATAATGGAAGGGTATGTCGGATGCATTGATCATATCGAGAAAGCCTATGAGCAAGGACATTGCATTCTGATGGAGCTTATAGATCGTGGCATGCTAAGACGCCAGGAGAATAATATCATGGGAGGAATCTTTTCAAGTATAGCTGATCACCGCCGTCGTGGGTTTACTGGGACTGCAAGCCTGGGATTGGTTAATGTGTTTGCAAATTCCAAGTGGGAAGGTCTTGGGAGGCTCACACACACTGATGGTATGATAAAATCGCCGTGCAGTGCTAAAACCTGGGGAAAAGTCTCTACACTTGTGATGGATGGACGCTGCCTCAGTAGAGAAGTCCCTGAAAAATATTTTCAACCAATGCAGGAGCTCCGGATATTAACTGTATTGAATCCCAGGTTCAGATCTCTACCCTTGTCCTTGACAAGCTTAAAGAACCTTTCTATTCTTGTGCTCAGATGCTGTGATCTCTTGGAGAAAATTGATCACCTCCGAGAATTTAAAAACTTAAATGTTCTTGAGATATCTGGTGCTACCTGCTTGGAGAAAATCGATGATGATCTTTTCACAGACATGCCTCACCTTCGAAGCCTTAACCTTTCTGGTGCCAATATCAAATCACTACCTGGTTCTCTCTTTCAAAAAACTGAACTACGGTGGCTCATCCTTAGAGGTTGTTCTCAATTGACAGAGCTTCCAAGCTTGAAATCGTTTGAAAACCTCCAGGTGCTTGATCTTCATGGTGCTACATCTTTCAAAAAGTTTCAAGACAAAACCTTTGCTCCGCTTCAGAAACTTCAGTTAATTGATCTTTCCAATTCCCAAATTAGTCGCCTACCTTTTCTTCACAATCTTGGTGATCTCACTCGGCTTCTATTAGCTGGTTGCAGAAGCTTAAAAAGACTGCCCATCTTGAACACACTAACTCAGCTTCAAATTCTTGATCTTTCGGGTGCTACAAGTTTACAAGAAATACAAAAAGAACCATTGGATGGCCTTAAAGTCCTTGATTTATCTCAGACTCAAATTAGTTGTCTGCCTTCAAGTGTTAGCAACCTTTCTGATCTTGTCTTAAGAGATTGTTCTCATCTTGTAAAACTGCCCCCCATAGGAGCAACTAAAGAGCTTGAACAGCTTGACCTTTCCGGCTCAAGAAATCTGGCTGAAATTGAAGACAAATCCTTACAACATATGAGGTTCCTTCGAGTTCTCAACTTTTCGAAAGCCAAAGTAAAAGAATTACCATCTTTGTCCAACCTAGTTAACCTTCGCCAACTCCTGTTAATGGACTGTACGGTTCTAGAGAAACTGCCTGCGATGGAGGGCCTTCTCAGACTCCAGGTTCTCAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCCCACCTGAATGCCCTTGAAAAATTAGAGATTCTTGATGTTTCAGGTTGTACGGCATTGAAAGGAATACATGAAAAGTCCTTTGAAAATATGTCATGTATTCGTACACTCAACCTCTCAGACTCTAAAATTGAGTTTCTACCGTCCCTTCCCAAAGCCAGAATCTTCTGTCATCTCATACTCAGAAATTGCAGCAACCTAAAAAAGCTCCCTCCTCTTGAACATCTGTCAAAACTTGAGGAACTTGATCTATGCGGTGCCATTTCTTTAGTTGACTTTGAAGCTGGGTTCTTGGAGCATATGGATGACCTTAGAATTCTCAACCTAACAGGAATCCCACTTACAATGCTACCGTCCATGTCCAGACTCACCAACCTCAGACAACTCGCACTAAAAGGTTGTTCGTCGATGGAAACAGTGCAAAACCTAGAAGCACTAACACTACTTGAGATTCTTGATCTTTCTGGAACAGCAATTGGGAGTCTGCCACCTCTAAACACTTTTAGCAACCTCGTCCAACTCTTGCTGAAAGATTGTTCACGGATAGAAGAGTTGCTGCTGCTAGGATCACTTAGTCGTCTGGAGGTTCTGGATCTTTCAGGAACCAGAATGAAAAAATTTCCCTATGAGATATCTGAATTGACTAATTTAAAGCACCTTTATCTTCCAGACGTGAGGAGTATGCATGATCTGGATGTTGGAAAGATAAAGCGTATACCACAAGAGGTCAACTGGGATCAATGTGGTATCTTTGAGCATGCTGATATCTTTTGTGACAATGACAAACCTTCCATATCTGTAAGTGGAACGGAAATTTTTCAGTTTTTGGACAAAAATCCCAAGGTGTGGGAAACAAAGTTCAAAAAATTTTGTTTCTTTGTAACCAAGAAGCAAGGTGAAGATGGAGATATCATCTTCTGCAAAGATGAAATTGTCAAAGATGTGTACTCAAGCACCAGACACTTGTGTTTTCCTGAAGAGCATGATCGGTCTTTGGAAATCCATGGATCATATGGTCTTCCTCATGGTTTCCCTCATGGTTTCAAAAGTGTCCTCAAGCATTCCGACTGCATATCTTTGGTTGACAATGACATTATAAGTTGCCTATCTGATTTAGGTGCTGACGTTGTGGAGGTAATGAAGGGCTGTTGGATAGAGAGATGCAGTAAAATTGAGAGCATTCTCTGTGGAGAAGAGGCAAATGTCAGACTGGGGAGAAGTTTAGAGATTCTATGGGTTTCGAACCTTCCGAAGTTGAGAAGTCTATACAATGGGAATGCGGAACCAGAGTGCTTTAAGAATCTTAAACAGTTGTTTCTAGATTGTTGTCCAATGATTGTGAATGTCTTTCCCTCATCCCAGCTTCCAGAAAACCTCGGAATTCTCCATGTGCAATTTTGTGATAGATTGCAAACTTTGGTTGAATGTGATACACCATCTAAGTATACATTCAAAAAGTTAAGGACACTGTATCTGTTGGAACTGCCAAAGTTGACGAGCATTGGGATTGAATTGCCAGATCAAGTGGATGTCGAAGCTATGGAATGCCCTAAGTTTACGAAGGACCCTCCTGCTATGATCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

1267

Amino Acids

143.08

Weight (kDa)

5.89

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 440 - 569 4.2e-08 Leucine-rich repeat region
LRR_8 PF13855 447 - 504 3.1e-08 Leucine rich repeat
LRR_14 PF23598 572 - 682 6.3e-06 Leucine-rich repeat region
LRR_14 PF23598 671 - 856 9.6e-06 Leucine-rich repeat region
LRR_14 PF23598 737 - 877 8.9e-06 Leucine-rich repeat region
LRR_14 PF23598 841 - 959 7e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 1150 - 1239 2.4e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3311
AasI GACNNNNNNGTC 1 cut(s) 173
AatII GACGTC 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 1379
AccB1I GGYRCC 2 cut(s) 1439, 2471
AccB7I CCANNNNNTGG 2 cut(s) 1826, 3060
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 3 cut(s) 488, 2929, 3659
AccIII TCCGGA 1 cut(s) 1206
AciI CCGC 7 cut(s) 249, 307, 753, 991, 1619, 2469, 3494
AclWI GGATC 4 cut(s) 2788, 2814, 2958, 3229
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 8 cut(s) 1045, 1331, 1770, 2529, 2831, 3027, 3080, 3594
AcuI CTGAAG 4 cut(s) 356, 1607, 1616, 3210
AcyI GRCGYC 2 cut(s) 174, 940
AfaI GTAC 4 cut(s) 2128, 2266, 2320, 3161
AfiI CCNNNNNNNGG 5 cut(s) 1027, 1826, 1943, 2119, 3060
AflII CTTAAG 2 cut(s) 113, 1906
AflIII ACRYGT 1 cut(s) 741
AjiI CACGTC 1 cut(s) 2887
AjnI CCWGG 8 cut(s) 285, 942, 1020, 1118, 1229, 1459, 1557, 2172
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AloI GAACNNNNNNTCC 2 cut(s) 3056, 3088
Alw21I GWGCWC 3 cut(s) 447, 1206, 1290
Alw26I GTCTC 2 cut(s) 45, 1135
AlwI GGATC 4 cut(s) 2788, 2814, 2958, 3229
AlwNI CAGNNNCTG 2 cut(s) 692, 1296
Aor13HI TCCGGA 1 cut(s) 1206
AoxI GGCC 3 cut(s) 127, 1828, 2158
ApoI RAATTY 8 cut(s) 1045, 1331, 1770, 2529, 2831, 3027, 3080, 3594
ArsI GACNNNNNNTTYG 4 cut(s) 1595, 1627, 1852, 1884
Asp700I GAANNNNTTC 2 cut(s) 970, 3030
AspLEI GCGC 2 cut(s) 411, 429
AspS9I GGNCC 2 cut(s) 2158, 3778
AsuC2I CCSGG 2 cut(s) 348, 549
AsuHPI GGTGA 8 cut(s) 410, 583, 980, 1313, 1406, 1703, 2575, 3122
AsuII TTCGAA 3 cut(s) 1420, 2054, 3455
AvaII GGWCC 1 cut(s) 3778
AxyI CCTNAGG 1 cut(s) 153
BanI GGYRCC 2 cut(s) 1439, 2471
BanII GRGCYC 1 cut(s) 1206
BbsI GAAGAC 4 cut(s) 270, 344, 2013, 3224
Bbv12I GWGCWC 3 cut(s) 447, 1206, 1290
BceAI ACGGC 4 cut(s) 42, 978, 1087, 2283
BciT130I CCWGG 8 cut(s) 287, 944, 1022, 1120, 1231, 1461, 1559, 2174
BclI TGATCA 5 cut(s) 828, 868, 985, 1318, 3792
BcnI CCSGG 2 cut(s) 348, 549
BcoDI GTCTC 2 cut(s) 45, 1135
BfaI CTAG 7 cut(s) 387, 600, 2093, 2135, 2642, 2777, 3533
BfmI CTRYAG 1 cut(s) 489
BfrI CTTAAG 2 cut(s) 113, 1906
BfuAI ACCTGC 1 cut(s) 1379
BglII AGATCT 1 cut(s) 1238
Bme18I GGWCC 1 cut(s) 3778
BmgBI CACGTC 1 cut(s) 2887
BmgT120I GGNCC 2 cut(s) 2158, 3778
BmiI GGNNCC 6 cut(s) 1441, 2033, 2473, 2818, 3498, 3780
BmrI ACTGGG 3 cut(s) 1017, 2956, 3434
BmsI GCATC 3 cut(s) 851, 1283, 3365
BmuI ACTGGG 3 cut(s) 1017, 2956, 3434
BpiI GAAGAC 4 cut(s) 270, 344, 2013, 3224
BpmI CTGGAG 3 cut(s) 1541, 2156, 2816
Bpu14I TTCGAA 3 cut(s) 1420, 2054, 3455
BpuEI CTTGAG 6 cut(s) 1373, 1969, 2471, 2681, 3148, 3254
BpuMI CCSGG 2 cut(s) 348, 549
Bsa29I ATCGAT 1 cut(s) 1386
BsaBI GATNNNNATC 2 cut(s) 244, 1392
BsaHI GRCGYC 2 cut(s) 174, 940
BsaJI CCNNGG 7 cut(s) 567, 1021, 1119, 1229, 3054, 3217, 3589
BsaWI WCCGGW 1 cut(s) 1206
BsaXI ACNNNNNCTCC 2 cut(s) 1600, 1630
Bsc4I CCNNNNNNNGG 5 cut(s) 1027, 1826, 1943, 2119, 3060
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 1012, 2951, 3429
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 3 cut(s) 120, 621, 898
Bse8I GATNNNNATC 2 cut(s) 244, 1392
BseAI TCCGGA 1 cut(s) 1206
BseBI CCWGG 8 cut(s) 287, 944, 1022, 1120, 1231, 1461, 1559, 2174
BseCI ATCGAT 1 cut(s) 1386
BseDI CCNNGG 7 cut(s) 567, 1021, 1119, 1229, 3054, 3217, 3589
BseJI GATNNNNATC 2 cut(s) 244, 1392
BseLI CCNNNNNNNGG 5 cut(s) 1027, 1826, 1943, 2119, 3060
BseMI GCAATG 3 cut(s) 120, 621, 898
BseNI ACTGG 3 cut(s) 1012, 2951, 3429
BseRI GAGGAG 7 cut(s) 200, 203, 434, 452, 455, 2418, 2905
BseYI CCCAGC 2 cut(s) 2498, 3573
BsgI GTGCAG 1 cut(s) 1126
BshFI GGCC 3 cut(s) 129, 1830, 2160
BshNI GGYRCC 2 cut(s) 1439, 2471
BshVI ATCGAT 1 cut(s) 1386
BsiHKAI GWGCWC 3 cut(s) 447, 1206, 1290
BsiSI CCGG 5 cut(s) 126, 347, 548, 1207, 1980
BslFI GGGAC 3 cut(s) 1024, 1157, 2344
BslI CCNNNNNNNGG 5 cut(s) 1027, 1826, 1943, 2119, 3060
BsmAI GTCTC 2 cut(s) 45, 1135
BsmFI GGGAC 3 cut(s) 1024, 1157, 2344
BsmI GAATGC 7 cut(s) 626, 903, 2230, 3274, 3393, 3496, 3764
BsnI GGCC 3 cut(s) 129, 1830, 2160
Bsp119I TTCGAA 3 cut(s) 1420, 2054, 3455
Bsp1286I GDGCHC 3 cut(s) 447, 1206, 1290
Bsp13I TCCGGA 1 cut(s) 1206
Bsp19I CCATGG 1 cut(s) 3217
BspACI CCGC 7 cut(s) 249, 307, 753, 991, 1619, 2469, 3494
BspANI GGCC 3 cut(s) 129, 1830, 2160
BspDI ATCGAT 1 cut(s) 1386
BspEI TCCGGA 1 cut(s) 1206
BspLI GGNNCC 6 cut(s) 1441, 2033, 2473, 2818, 3498, 3780
BspMI ACCTGC 1 cut(s) 1379
BspPI GGATC 4 cut(s) 2788, 2814, 2958, 3229
BspQI GCTCTTC 1 cut(s) 3186
BspT104I TTCGAA 3 cut(s) 1420, 2054, 3455
BspT107I GGYRCC 2 cut(s) 1439, 2471
BspTI CTTAAG 2 cut(s) 113, 1906
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 3 cut(s) 120, 621, 898
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 1012, 2951, 3429
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 7 cut(s) 567, 1021, 1119, 1229, 3054, 3217, 3589
BssNAI GTATAC 2 cut(s) 2930, 3660
BssNI GRCGYC 2 cut(s) 174, 940
BssT1I CCWWGG 3 cut(s) 567, 3054, 3217
Bst1107I GTATAC 2 cut(s) 2930, 3660
Bst2UI CCWGG 8 cut(s) 287, 944, 1022, 1120, 1231, 1461, 1559, 2174
Bst6I CTCTTC 5 cut(s) 41, 489, 2757, 3186, 3402
BstACI GRCGYC 2 cut(s) 174, 940
BstAFI CTTAAG 2 cut(s) 113, 1906
BstAPI GCANNNNNTGC 1 cut(s) 784
BstBI TTCGAA 3 cut(s) 1420, 2054, 3455
BstC8I GCNNGC 7 cut(s) 127, 131, 785, 932, 1018, 2148, 2973
BstDSI CCRYGG 1 cut(s) 3217
BstHHI GCGC 2 cut(s) 411, 429
BstMAI GTCTC 2 cut(s) 45, 1135
BstMWI GCNNNNNNNGC 2 cut(s) 784, 1966
BstNI CCWGG 8 cut(s) 287, 944, 1022, 1120, 1231, 1461, 1559, 2174
BstNSI RCATGY 4 cut(s) 787, 934, 1411, 2975
BstSFI CTRYAG 1 cut(s) 489
BstV2I GAAGAC 4 cut(s) 270, 344, 2013, 3224
BstX2I RGATCY 2 cut(s) 1238, 2806
BstYI RGATCY 2 cut(s) 1238, 2806
BstZ17I GTATAC 2 cut(s) 2930, 3660
Bsu15I ATCGAT 1 cut(s) 1386
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 3 cut(s) 129, 1830, 2160
BsuTUI ATCGAT 1 cut(s) 1386
BtgI CCRYGG 1 cut(s) 3217
BtgZI GCGATG 2 cut(s) 595, 2165
BtrI CACGTC 1 cut(s) 2887
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 4 cut(s) 1080, 1114, 2637, 3680
BveI ACCTGC 1 cut(s) 1379
Cac8I GCNNGC 7 cut(s) 127, 131, 785, 932, 1018, 2148, 2973
CaiI CAGNNNCTG 2 cut(s) 692, 1296
CfoI GCGC 2 cut(s) 411, 429
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 2158, 3778
ClaI ATCGAT 1 cut(s) 1386
CseI GACGC 3 cut(s) 349, 948, 1161
CsiI ACCWGGT 1 cut(s) 1459
Csp6I GTAC 4 cut(s) 2127, 2265, 2319, 3160
CviQI GTAC 4 cut(s) 2127, 2265, 2319, 3160
DraI TTTAAA 2 cut(s) 1336, 2865
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 5 cut(s) 41, 489, 2757, 3186, 3402
EarI CTCTTC 5 cut(s) 41, 489, 2757, 3186, 3402
Ecl136II GAGCTC 1 cut(s) 1204
Eco130I CCWWGG 3 cut(s) 567, 3054, 3217
Eco24I GRGCYC 1 cut(s) 1206
Eco32I GATATC 5 cut(s) 246, 1359, 2847, 2980, 3124
Eco47I GGWCC 1 cut(s) 3778
Eco53kI GAGCTC 1 cut(s) 1204
Eco57I CTGAAG 4 cut(s) 356, 1607, 1616, 3210
Eco81I CCTNAGG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 1204
EcoO109I RGGNCCY 2 cut(s) 2158, 3778
EcoRI GAATTC 2 cut(s) 2529, 3594
EcoRII CCWGG 8 cut(s) 285, 942, 1020, 1118, 1229, 1459, 1557, 2172
EcoRV GATATC 5 cut(s) 246, 1359, 2847, 2980, 3124
EcoT14I CCWWGG 3 cut(s) 567, 3054, 3217
EcoT22I ATGCAT 2 cut(s) 866, 2901
EcoT38I GRGCYC 1 cut(s) 1206
ErhI CCWWGG 3 cut(s) 567, 3054, 3217
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 3 cut(s) 1024, 1157, 2344
FauNDI CATATG 3 cut(s) 2025, 2514, 3226
FbaI TGATCA 5 cut(s) 828, 868, 985, 1318, 3792
FblI GTMKAC 3 cut(s) 488, 2929, 3659
FriOI GRGCYC 1 cut(s) 1206
FspBI CTAG 7 cut(s) 387, 600, 2093, 2135, 2642, 2777, 3533
GlaI GCGC 2 cut(s) 410, 428
GsaI CCCAGC 2 cut(s) 2502, 3577
GsuI CTGGAG 3 cut(s) 1541, 2156, 2816
HaeIII GGCC 3 cut(s) 129, 1830, 2160
HapII CCGG 5 cut(s) 126, 347, 548, 1207, 1980
HgaI GACGC 3 cut(s) 349, 948, 1161
HhaI GCGC 2 cut(s) 411, 429
Hin1I GRCGYC 2 cut(s) 174, 940
Hin6I GCGC 2 cut(s) 409, 427
HinP1I GCGC 2 cut(s) 409, 427
HincII GTYRAC 5 cut(s) 2098, 2488, 2944, 3298, 3708
HindII GTYRAC 5 cut(s) 2098, 2488, 2944, 3298, 3708
HindIII AAGCTT 4 cut(s) 503, 1261, 1534, 1726
HpaI GTTAAC 1 cut(s) 2098
HpaII CCGG 5 cut(s) 126, 347, 548, 1207, 1980
HphI GGTGA 8 cut(s) 410, 583, 980, 1313, 1406, 1703, 2575, 3122
Hpy99I CGWCG 2 cut(s) 999, 2623
HpyCH4IV ACGT 6 cut(s) 20, 174, 474, 743, 2886, 3339
HpyF10VI GCNNNNNNNGC 2 cut(s) 784, 1966
HpySE526I ACGT 6 cut(s) 20, 174, 474, 743, 2886, 3339
Hsp92I GRCGYC 2 cut(s) 174, 940
HspAI GCGC 2 cut(s) 409, 427
Kpn2I TCCGGA 1 cut(s) 1206
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Ksp22I TGATCA 5 cut(s) 828, 868, 985, 1318, 3792
KspAI GTTAAC 1 cut(s) 2098
LguI GCTCTTC 1 cut(s) 3186
LweI GCATC 3 cut(s) 851, 1283, 3365
MabI ACCWGGT 1 cut(s) 1459
MaeI CTAG 7 cut(s) 387, 600, 2093, 2135, 2642, 2777, 3533
MaeII ACGT 6 cut(s) 20, 174, 474, 743, 2886, 3339
MaeIII GTNAC 3 cut(s) 398, 2987, 3094
MbiI CCGCTC 1 cut(s) 307
MfeI CAATTG 3 cut(s) 482, 1517, 2688
MflI RGATCY 2 cut(s) 1238, 2806
MhlI GDGCHC 3 cut(s) 447, 1206, 1290
MlyI GAGTC 7 cut(s) 506, 536, 1849, 2163, 2330, 2574, 2704
MmeI TCCRAC 9 cut(s) 183, 564, 838, 2112, 2755, 2893, 3303, 3344, 3672
Mph1103I ATGCAT 2 cut(s) 866, 2901
MroI TCCGGA 1 cut(s) 1206
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 2 cut(s) 970, 3030
MslI CAYNNNNRTG 6 cut(s) 614, 908, 1083, 1406, 2223, 2517
MspA1I CMGCKG 2 cut(s) 692, 3798
MspCI CTTAAG 2 cut(s) 113, 1906
MspI CCGG 5 cut(s) 126, 347, 548, 1207, 1980
MunI CAATTG 3 cut(s) 482, 1517, 2688
Mva1269I GAATGC 7 cut(s) 626, 903, 2230, 3274, 3393, 3496, 3764
MvaI CCWGG 8 cut(s) 287, 944, 1022, 1120, 1231, 1461, 1559, 2174
MwoI GCNNNNNNNGC 2 cut(s) 784, 1966
NaeI GCCGGC 1 cut(s) 127
NciI CCSGG 2 cut(s) 348, 549
NcoI CCATGG 1 cut(s) 3217
NdeI CATATG 3 cut(s) 2025, 2514, 3226
NgoMIV GCCGGC 1 cut(s) 125
NlaIV GGNNCC 6 cut(s) 1441, 2033, 2473, 2818, 3498, 3780
NmeAIII GCCGAG 1 cut(s) 1681
NmuCI GTSAC 2 cut(s) 398, 2987
NsiI ATGCAT 2 cut(s) 866, 2901
NspI RCATGY 4 cut(s) 787, 934, 1411, 2975
NspV TTCGAA 3 cut(s) 1420, 2054, 3455
PaeI GCATGC 3 cut(s) 787, 934, 2975
PciSI GCTCTTC 1 cut(s) 3186
PcsI WCGNNNNNNNCGW 1 cut(s) 3461
PctI GAATGC 7 cut(s) 626, 903, 2230, 3274, 3393, 3496, 3764
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 970, 3030
PfeI GAWTC 8 cut(s) 966, 1225, 2245, 2376, 2547, 2665, 3442, 3514
PflMI CCANNNNNTGG 2 cut(s) 1826, 3060
PfoI TCCNGGA 1 cut(s) 285
PleI GAGTC 7 cut(s) 505, 536, 1849, 2163, 2330, 2574, 2703
PpsI GAGTC 7 cut(s) 505, 536, 1849, 2163, 2330, 2574, 2703
PpuMI RGGWCCY 1 cut(s) 3778
PsiI TTATAA 1 cut(s) 3311
Psp124BI GAGCTC 1 cut(s) 1206
Psp5II RGGWCCY 1 cut(s) 3778
Psp6I CCWGG 8 cut(s) 285, 942, 1020, 1118, 1229, 1459, 1557, 2172
PspFI CCCAGC 2 cut(s) 2498, 3573
PspGI CCWGG 8 cut(s) 285, 942, 1020, 1118, 1229, 1459, 1557, 2172
PspN4I GGNNCC 6 cut(s) 1441, 2033, 2473, 2818, 3498, 3780
PspPI GGNCC 2 cut(s) 2158, 3778
PspPPI RGGWCCY 1 cut(s) 3778
PstNI CAGNNNCTG 2 cut(s) 692, 1296
PsuI RGATCY 2 cut(s) 1238, 2806
PvuII CAGCTG 2 cut(s) 692, 3798
RsaI GTAC 4 cut(s) 2128, 2266, 2320, 3161
RsaNI GTAC 4 cut(s) 2127, 2265, 2319, 3160
RseI CAYNNNNRTG 6 cut(s) 614, 908, 1083, 1406, 2223, 2517
SacI GAGCTC 1 cut(s) 1206
SapI GCTCTTC 1 cut(s) 3186
Sau96I GGNCC 2 cut(s) 2158, 3778
SchI GAGTC 7 cut(s) 506, 536, 1849, 2163, 2330, 2574, 2704
SduI GDGCHC 3 cut(s) 447, 1206, 1290
SexAI ACCWGGT 1 cut(s) 1459
SfaNI GCATC 3 cut(s) 851, 1283, 3365
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 3 cut(s) 1420, 2054, 3455
SinI GGWCC 1 cut(s) 3778
SmiMI CAYNNNNRTG 6 cut(s) 614, 908, 1083, 1406, 2223, 2517
SmlI CTYRAG 8 cut(s) 113, 1352, 1906, 1984, 2450, 2660, 3163, 3269
SmoI CTYRAG 8 cut(s) 113, 1352, 1906, 1984, 2450, 2660, 3163, 3269
SphI GCATGC 3 cut(s) 787, 934, 2975
SsiI CCGC 7 cut(s) 249, 307, 753, 991, 1619, 2469, 3494
SspI AATATT 2 cut(s) 272, 1184
SspMI CTAG 7 cut(s) 387, 600, 2093, 2135, 2642, 2777, 3533
SstI GAGCTC 1 cut(s) 1206
StyI CCWWGG 3 cut(s) 567, 3054, 3217
TaiI ACGT 6 cut(s) 23, 177, 477, 746, 2889, 3342
TaqI TCGA 8 cut(s) 876, 1386, 1420, 2038, 2054, 2621, 3455, 3747
TaqII GACCGA 1 cut(s) 3192
TatI WGTACW 1 cut(s) 3159
TfiI GAWTC 8 cut(s) 966, 1225, 2245, 2376, 2547, 2665, 3442, 3514
TscAI CASTG 4 cut(s) 1087, 1114, 2637, 3687
TseFI GTSAC 2 cut(s) 398, 2987
Tsp45I GTSAC 2 cut(s) 398, 2987
TspDTI ATGAA 6 cut(s) 59, 1562, 2301, 2840, 3156, 3368
TspGWI ACGGA 3 cut(s) 462, 2770, 3038
TspRI CASTG 4 cut(s) 1087, 1114, 2637, 3687
Van91I CCANNNNNTGG 2 cut(s) 1826, 3060
Vha464I CTTAAG 2 cut(s) 113, 1906
VpaK11BI GGWCC 1 cut(s) 3778
XapI RAATTY 8 cut(s) 1045, 1331, 1770, 2529, 2831, 3027, 3080, 3594
XbaI TCTAGA 2 cut(s) 2134, 3532
XceI RCATGY 4 cut(s) 787, 934, 1411, 2975
XcmI CCANNNNNNNNNTGG 2 cut(s) 574, 3224
XmiI GTMKAC 3 cut(s) 488, 2929, 3659
XmnI GAANNNNTTC 2 cut(s) 970, 3030
XspI CTAG 7 cut(s) 387, 600, 2093, 2135, 2642, 2777, 3533
ZraI GACGTC 1 cut(s) 175
Zsp2I ATGCAT 2 cut(s) 866, 2901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.