RchiOBHm_Chr3g0479451

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009232
Physical Location & Seq
Reverse (-)
25324236 .. 25327235
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44453

Sequence Viewer

Length: 2889 bp
ATGTCAATGGTCTTAGGTACAAAGGAGGAACATAAAAAGAAGATATTGGAGCTGTTGAAGGATCCCAATGTGTCAACAGTAGTTCTTGATGGGAAGCGTGGAGTAGGGAAAACGAGGATAGCAAAAGAGATAATCATGGATGAAGATTTCCATGGCACTCTCTGGCTGTATGTGAATGACAATTATGACACAGAATCTCTTCACAAAAACATTGCTCGCCAGCTATCTCTTTTCTCTATTTATGAGGAATGGGAAGATGATGATGCTGTCGAGGAGGAGCAGAGCTTGGAAAAGTTGAAGCCGAGAATATCTGTGAAGCTTGAAGAATTCAGATCTGCAGCACATGAAAAACATAAACTGTTTCTTCTAGTTCTGGATGATGTTCCTCATCAGAAGGATGTAGAGGAGATTGTGGAAGAAGTTAACAACCTGCTGACAAATGACCGCTCGTCTTTCAAGGTTTTAATCACCAGAGGCACCACACTCGGGATGAACCAGGAAATTAAGAGCTGCAATACAAAAGTACGCCAGATAAATCCATTGTCCACGGATGAGTCCTTGGCTTTATTGCGTGAAAGAACCAAGAAAGAAGTTTTTGATCGTCCAAGCTTTGAAAAACTATCCACAGCTATTGTGGAGATGAGTAAGGGTATTCCTACCATACTTATCACCATAGCAAAAGCAATAAATTACCTGGAAGATGAATGTTCTGGCGTTTGGTCGTTGGAAAATGTTTTGGAAGAAGCAGCTTATGATGGGGAAACTGCTATAAATCTGCTACTTCGCTCTTGGTGTAATGCATTGCCAACCGGTGCTCCGACCGACTGCTTTTGGCATACCCTGCAGCTTCTCAACAAACATGGTGGTGTTCATTACAATGAGTTGATCACACAATGGATAATGGAAGGATATTTTGGATGCAACAACCAGATTGGGAAGGTCTATGAGCAAGGACATAAGGTTCTGATGGACCTTATAGATTGGGGCATGCTGACAAAACAAGAGGATAATATGGTTGTCATGGGAGGAATAATTTTGAGTATAACTGGTCATTGCCGACGTGAATTGAATGAGACTGCAAGGCTTGGATTGGCTAATGTGTTTGAAGATGAAAATTGGAAAGGTCTTGGAATGCTTGCACATACGGATCGTATGATAAAATCACCATCATGTCATAAGACCTGGGAGAAAGTTTCCACACTTCTGATGGATGGGCATTGCATTGATAGGGAAGTACCTGTAACATGCTTTCAACAAATGAAGACTCTCCAAGTTCTCATGTTAATCAAGCCTAGGTTCTCTCTGCTAGTACCCTTGGTTGGCATAAATAACCTTTCCATTCTTGTGCTTAGATTCTGTGATCGCTTGGAGAAGATTGATCCTATCCGAGAATTTCAAAGCTTAAGTGTTCTTGAGATATCTGGTGCTACTTTGTTGGAGATGATCCCCAATGACTTTTTTACAAAGATGCATAAGCTTCGAAGCGTTAACCTTTCTGAAGCCAAGGTCAAATCACTACCTAGTTCTCTTTTTGAAAGAAGTGAACTACGGTGGCTCATCCTTAGAGGATGTTCTCAATTGAAAACACTTCCAAGCTTAAAATCCTTGGGAAAGCTCAAGGTGCTTGATCTTTCTGGTGCTACCTCTTTCAGCAGATTCCAAGACAAAACCTTTGCAGCTCTTCAGAAACTTCATACACTTAATCTTTCTAATTCTCAAATTACTCGCTTACCTTTTCTTCATGGTAATGATGAGCTTAATCAGCTCCTATTAGGTGGCTGTGCAAGTTTAAACAGACTTCCAACCTTGAACACAATACCTCGGCTTCAAATTCTAGATCTTTCTGGTGCTACAGGCTTAAAAGAAATACAGTTCGAACCTTTAGATAGCCTTAAGGCCCTTGATTTATCTGGAACTCGTATTGAAAGGCAACCTTCCAGTTTCAGCCACCTTTCTGATCTCTCCTTACGAGAGTGTCATGAGCTTGCAAGACTTCCACTCACAAAAGAAATCAAATATCTTGAATCACTTGACCTTTCTGGTTCTAGCACTAAGGTTGAAATTGAAGACAAATTCTTCCAGCATATGAGGTTTCTTCGAGTTCTCAACTTTTCAACAGTTAAGATAAAAGAACTACCTTCCCTGTCCAACCTAGCTAACCTCTGTCAACTTTTCCTAAAGGATTGTTTATGTTTAGAAAAGCTTCCAGAGATGGAAGGACTTAAAAGGCTCCAAGAGCTTAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCAAACCTGAACTCCCTTGAAAAACTGGAGATTCTTGATATTTCACGTTGCAGGGTTTTGAACCAAATACAAGAGGAATCCTTTCAAAAGATGTCCCATATTCGGACACTCAACCTCTCAGACACTATGATTGAGTTCCTACCATCCATTTGCAAACCCAGCAACCTCTGTCACCTCATCCTCAGAAATTGCAAAAAGTTAAAAAAGCTTCCACCTCTTGATCATTTGTCAAAACTTGAGGAATTGAATCTGTGTGGTGCCAGTTCTTTAGTTGACTTTACATCTGAATTCCTGGAGCCTATGGTCGACCTTCAAATTGTTGACTTATCTGAAATCCCACTGACAAAGGTACCATCCATGTCCAAATTCATCAAACTCAGACAGTTCTCAGTAAAGGGTTGTTCATCCTTAAATGAAGTGATACATCTTGAAGAACTAACACTTCTTGAGATTCTTGATCTTTCTGGAACAGCAGTTCCGAGTCTGCCTCCCCTAAATACTTTTAGCAACCTCCGCCAGCTCTTGCTAAAAGATTGTTTACAGATAGAAGAGTTGCAGTTTCTAGGGTCGCTTAGTCGGCTGGAGGTTCTTGATCTCTTCATCTGCCTGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

962

Amino Acids

109.2

Weight (kDa)

5.83

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 9 - 193 2.5e-12 NB-ARC domain
LRR_14 PF23598 456 - 573 5.7e-07 Leucine-rich repeat region
LRR_14 PF23598 695 - 841 7.8e-07 Leucine-rich repeat region
LRR_EndoS PF23952 697 - 780 7.2e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_14 PF23598 757 - 890 6.9e-06 Leucine-rich repeat region
LRR_8 PF13855 766 - 822 3.9e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 438
Acc65I GGTACC 1 cut(s) 2623
AccB1I GGYRCC 3 cut(s) 476, 2531, 2623
AccBSI CCGCTC 1 cut(s) 447
AccI GTMKAC 1 cut(s) 2580
AciI CCGC 2 cut(s) 445, 2788
AclWI GGATC 5 cut(s) 56, 69, 1155, 1373, 1438
AcsI RAATTY 6 cut(s) 326, 1391, 1830, 2072, 2561, 2639
AcuI CTGAAG 2 cut(s) 1518, 1667
AfaI GTAC 5 cut(s) 19, 525, 1236, 1311, 2625
AfiI CCNNNNNNNGG 3 cut(s) 486, 1319, 2376
AflII CTTAAG 2 cut(s) 1402, 1892
AgeI ACCGGT 1 cut(s) 809
AhdI GACNNNNNGTC 1 cut(s) 448
AjiI CACGTC 1 cut(s) 1061
AjnI CCWGG 4 cut(s) 495, 693, 1181, 2565
AjuI GAANNNNNNNTTGG 2 cut(s) 897, 929
AloI GAACNNNNNNTCC 6 cut(s) 945, 977, 2733, 2734, 2765, 2766
Alw21I GWGCWC 1 cut(s) 817
Alw26I GTCTC 1 cut(s) 1067
AlwI GGATC 5 cut(s) 56, 69, 1155, 1373, 1438
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 1 cut(s) 1896
ApeKI GCWGC 5 cut(s) 338, 510, 746, 844, 1676
ApoI RAATTY 6 cut(s) 326, 1391, 1830, 2072, 2561, 2639
ArsI GACNNNNNNTTYG 2 cut(s) 1655, 1687
AsiGI ACCGGT 1 cut(s) 809
Asp700I GAANNNNTTC 3 cut(s) 198, 2202, 2355
Asp718I GGTACC 1 cut(s) 2623
AspA2I CCTAGG 1 cut(s) 1292
AspS9I GGNCC 2 cut(s) 970, 1897
AsuHPI GGTGA 4 cut(s) 460, 661, 1155, 2438
AsuII TTCGAA 2 cut(s) 1480, 1875
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 1 cut(s) 970
AvrII CCTAGG 1 cut(s) 1292
BaeI ACNNNNGTAYC 2 cut(s) 2607, 2640
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 3 cut(s) 476, 2531, 2623
BbsI GAAGAC 2 cut(s) 1268, 2073
Bbv12I GWGCWC 1 cut(s) 817
BbvI GCAGC 5 cut(s) 350, 497, 758, 856, 1688
BccI CCATC 9 cut(s) 83, 749, 961, 1174, 1201, 1205, 2206, 2425, 2635
BcgI CGANNNNNNTGC 4 cut(s) 466, 500, 1460, 1494
BciT130I CCWGG 4 cut(s) 497, 695, 1183, 2567
BclI TGATCA 2 cut(s) 885, 2494
BcoDI GTCTC 1 cut(s) 1067
BfaI CTAG 8 cut(s) 368, 1293, 1307, 1521, 1835, 2046, 2153, 2837
BfmI CTRYAG 3 cut(s) 336, 842, 1851
BfrI CTTAAG 2 cut(s) 1402, 1892
BfuAI ACCTGC 1 cut(s) 438
BglII AGATCT 2 cut(s) 332, 1837
BisI GCNGC 5 cut(s) 339, 511, 747, 845, 1677
BlnI CCTAGG 1 cut(s) 1292
BlsI GCNGC 5 cut(s) 340, 512, 748, 846, 1678
Bme1390I CCNGG 4 cut(s) 497, 695, 1183, 2567
Bme18I GGWCC 1 cut(s) 970
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 485
BmgBI CACGTC 1 cut(s) 1061
BmgT120I GGNCC 2 cut(s) 970, 1897
BmiI GGNNCC 6 cut(s) 63, 478, 2231, 2533, 2571, 2625
BmrFI CCNGG 4 cut(s) 497, 695, 1183, 2567
BmsI GCATC 3 cut(s) 253, 908, 1458
BpiI GAAGAC 2 cut(s) 1268, 2073
BplI GAGNNNNNCTC 2 cut(s) 2746, 2778
BpmI CTGGAG 3 cut(s) 2321, 2588, 2876
Bpu14I TTCGAA 2 cut(s) 1480, 1875
BpuEI CTTGAG 4 cut(s) 1433, 1601, 2531, 2741
BsaJI CCNNGG 9 cut(s) 151, 546, 558, 1182, 1292, 1314, 1503, 1605, 1820
BsaWI WCCGGW 1 cut(s) 809
BsaXI ACNNNNNCTCC 4 cut(s) 799, 829, 2270, 2300
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 1319, 2376
Bse118I RCCGGY 1 cut(s) 809
Bse1I ACTGG 4 cut(s) 1051, 1938, 2304, 2535
Bse3DI GCAATG 4 cut(s) 210, 800, 1051, 1216
BseBI CCWGG 4 cut(s) 497, 695, 1183, 2567
BseDI CCNNGG 9 cut(s) 151, 546, 558, 1182, 1292, 1314, 1503, 1605, 1820
BseLI CCNNNNNNNGG 3 cut(s) 486, 1319, 2376
BseMI GCAATG 4 cut(s) 210, 800, 1051, 1216
BseMII CTCAG 4 cut(s) 2406, 2470, 2665, 2676
BseNI ACTGG 4 cut(s) 1051, 1938, 2304, 2535
BseRI GAGGAG 3 cut(s) 287, 290, 419
BseXI GCAGC 5 cut(s) 350, 497, 758, 856, 1688
BseYI CCCAGC 1 cut(s) 2432
Bsh1285I CGRYCG 1 cut(s) 822
BshFI GGCC 1 cut(s) 1898
BshNI GGYRCC 3 cut(s) 476, 2531, 2623
BshTI ACCGGT 1 cut(s) 809
BsiEI CGRYCG 1 cut(s) 822
BsiHKAI GWGCWC 1 cut(s) 817
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 1 cut(s) 810
BslFI GGGAC 1 cut(s) 2353
BslI CCNNNNNNNGG 3 cut(s) 486, 1319, 2376
BsmAI GTCTC 1 cut(s) 1067
BsmFI GGGAC 1 cut(s) 2353
BsmI GAATGC 1 cut(s) 1137
BsnI GGCC 1 cut(s) 1898
BsoBI CYCGRG 1 cut(s) 485
Bsp119I TTCGAA 2 cut(s) 1480, 1875
Bsp1286I GDGCHC 1 cut(s) 817
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 2 cut(s) 445, 2788
BspANI GGCC 1 cut(s) 1898
BspCNI CTCAG 4 cut(s) 2405, 2469, 2664, 2675
BspHI TCATGA 1 cut(s) 1978
BspLI GGNNCC 6 cut(s) 63, 478, 2231, 2533, 2571, 2625
BspMAI CTGCAG 2 cut(s) 340, 846
BspMI ACCTGC 1 cut(s) 438
BspPI GGATC 5 cut(s) 56, 69, 1155, 1373, 1438
BspQI GCTCTTC 1 cut(s) 1686
BspT104I TTCGAA 2 cut(s) 1480, 1875
BspT107I GGYRCC 3 cut(s) 476, 2531, 2623
BspTI CTTAAG 2 cut(s) 1402, 1892
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 4 cut(s) 210, 800, 1051, 1216
BsrFI RCCGGY 1 cut(s) 809
BsrI ACTGG 4 cut(s) 1051, 1938, 2304, 2535
BssAI RCCGGY 1 cut(s) 809
BssECI CCNNGG 9 cut(s) 151, 546, 558, 1182, 1292, 1314, 1503, 1605, 1820
BssT1I CCWWGG 6 cut(s) 151, 558, 1292, 1314, 1503, 1605
Bst2UI CCWGG 4 cut(s) 497, 695, 1183, 2567
Bst4CI ACNGT 6 cut(s) 79, 360, 1551, 1872, 2119, 2658
Bst6I CTCTTC 4 cut(s) 204, 1686, 2817, 2876
BstAFI CTTAAG 2 cut(s) 1402, 1892
BstAPI GCANNNNNTGC 1 cut(s) 841
BstBI TTCGAA 2 cut(s) 1480, 1875
BstC8I GCNNGC 6 cut(s) 217, 221, 989, 1137, 1986, 2792
BstDEI CTNAG 9 cut(s) 13, 1349, 1562, 2052, 2392, 2456, 2651, 2662, 2846
BstDSI CCRYGG 2 cut(s) 151, 546
BstMAI GTCTC 1 cut(s) 1067
BstMCI CGRYCG 1 cut(s) 822
BstMWI GCNNNNNNNGC 7 cut(s) 841, 1621, 1762, 2236, 2433, 2787, 2851
BstNI CCWGG 4 cut(s) 497, 695, 1183, 2567
BstNSI RCATGY 2 cut(s) 991, 1248
BstSCI CCNGG 4 cut(s) 495, 693, 1181, 2565
BstSFI CTRYAG 3 cut(s) 336, 842, 1851
BstV1I GCAGC 5 cut(s) 350, 497, 758, 856, 1688
BstV2I GAAGAC 2 cut(s) 1268, 2073
BstX2I RGATCY 3 cut(s) 61, 332, 1837
BstYI RGATCY 3 cut(s) 61, 332, 1837
BsuRI GGCC 1 cut(s) 1898
BtgI CCRYGG 2 cut(s) 151, 546
BtrI CACGTC 1 cut(s) 1061
BtsIMutI CAGTG 1 cut(s) 2612
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 6 cut(s) 217, 221, 989, 1137, 1986, 2792
CciI TCATGA 1 cut(s) 1978
Cfr10I RCCGGY 1 cut(s) 809
Cfr13I GGNCC 2 cut(s) 970, 1897
Csp6I GTAC 5 cut(s) 18, 524, 1235, 1310, 2624
CspAI ACCGGT 1 cut(s) 809
CspCI CAANNNNNGTGG 4 cut(s) 613, 648, 844, 879
CviQI GTAC 5 cut(s) 18, 524, 1235, 1310, 2624
DdeI CTNAG 9 cut(s) 13, 1349, 1562, 2052, 2392, 2456, 2651, 2662, 2846
DraI TTTAAA 1 cut(s) 1791
DriI GACNNNNNGTC 1 cut(s) 448
Eam1104I CTCTTC 4 cut(s) 204, 1686, 2817, 2876
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 4 cut(s) 204, 1686, 2817, 2876
EciI GGCGGA 1 cut(s) 2777
Eco130I CCWWGG 6 cut(s) 151, 558, 1292, 1314, 1503, 1605
Eco32I GATATC 1 cut(s) 1419
Eco47I GGWCC 1 cut(s) 970
Eco57I CTGAAG 2 cut(s) 1518, 1667
Eco88I CYCGRG 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 1897
EcoRI GAATTC 2 cut(s) 326, 2561
EcoRII CCWGG 4 cut(s) 495, 693, 1181, 2565
EcoRV GATATC 1 cut(s) 1419
EcoT14I CCWWGG 6 cut(s) 151, 558, 1292, 1314, 1503, 1605
EcoT22I ATGCAT 2 cut(s) 802, 1473
ErhI CCWWGG 6 cut(s) 151, 558, 1292, 1314, 1503, 1605
FalI AAGNNNNNCTT 2 cut(s) 1918, 1950
FaqI GGGAC 1 cut(s) 2353
FauNDI CATATG 1 cut(s) 2085
FbaI TGATCA 2 cut(s) 885, 2494
FblI GTMKAC 1 cut(s) 2580
Fnu4HI GCNGC 5 cut(s) 339, 511, 747, 845, 1677
Fsp4HI GCNGC 5 cut(s) 339, 511, 747, 845, 1677
FspBI CTAG 8 cut(s) 368, 1293, 1307, 1521, 1835, 2046, 2153, 2837
GluI GCNGC 5 cut(s) 339, 511, 747, 845, 1677
GsaI CCCAGC 1 cut(s) 2436
GsuI CTGGAG 3 cut(s) 2321, 2588, 2876
HaeIII GGCC 1 cut(s) 1898
HapII CCGG 1 cut(s) 810
HincII GTYRAC 7 cut(s) 75, 424, 1489, 2168, 2548, 2581, 2596
HindII GTYRAC 7 cut(s) 75, 424, 1489, 2168, 2548, 2581, 2596
HindIII AAGCTT 7 cut(s) 317, 607, 1399, 1475, 1594, 2201, 2480
HpaI GTTAAC 2 cut(s) 424, 1489
HpaII CCGG 1 cut(s) 810
HphI GGTGA 4 cut(s) 460, 661, 1155, 2438
Hpy99I CGWCG 1 cut(s) 1062
HpyAV CCTTC 8 cut(s) 52, 388, 899, 931, 1944, 2148, 2210, 2594
HpyCH4III ACNGT 6 cut(s) 79, 360, 1551, 1872, 2119, 2658
HpyCH4IV ACGT 2 cut(s) 1060, 2320
HpyF10VI GCNNNNNNNGC 7 cut(s) 841, 1621, 1762, 2236, 2433, 2787, 2851
HpyF3I CTNAG 9 cut(s) 13, 1349, 1562, 2052, 2392, 2456, 2651, 2662, 2846
HpySE526I ACGT 2 cut(s) 1060, 2320
KpnI GGTACC 1 cut(s) 2627
Ksp22I TGATCA 2 cut(s) 885, 2494
KspAI GTTAAC 2 cut(s) 424, 1489
LguI GCTCTTC 1 cut(s) 1686
LmnI GCTCC 6 cut(s) 49, 277, 820, 1770, 2235, 2569
Lsp1109I GCAGC 5 cut(s) 350, 497, 758, 856, 1688
LweI GCATC 3 cut(s) 253, 908, 1458
MaeI CTAG 8 cut(s) 368, 1293, 1307, 1521, 1835, 2046, 2153, 2837
MaeII ACGT 2 cut(s) 1060, 2320
MaeIII GTNAC 2 cut(s) 1240, 2444
MbiI CCGCTC 1 cut(s) 447
MfeI CAATTG 1 cut(s) 1577
MflI RGATCY 3 cut(s) 61, 332, 1837
MhlI GDGCHC 1 cut(s) 817
MlyI GAGTC 3 cut(s) 563, 1258, 2764
MmeI TCCRAC 5 cut(s) 705, 842, 1416, 1826, 2172
Mph1103I ATGCAT 2 cut(s) 802, 1473
MroXI GAANNNNTTC 3 cut(s) 198, 2202, 2355
MslI CAYNNNNRTG 5 cut(s) 864, 876, 1168, 1343, 1746
MspCI CTTAAG 2 cut(s) 1402, 1892
MspI CCGG 1 cut(s) 810
MspR9I CCNGG 4 cut(s) 497, 695, 1183, 2567
MssI GTTTAAAC 1 cut(s) 1791
MunI CAATTG 1 cut(s) 1577
Mva1269I GAATGC 1 cut(s) 1137
MvaI CCWGG 4 cut(s) 497, 695, 1183, 2567
MwoI GCNNNNNNNGC 7 cut(s) 841, 1621, 1762, 2236, 2433, 2787, 2851
NcoI CCATGG 1 cut(s) 151
NdeI CATATG 1 cut(s) 2085
NlaIV GGNNCC 6 cut(s) 63, 478, 2231, 2533, 2571, 2625
NmeAIII GCCGAG 2 cut(s) 327, 1801
NmuCI GTSAC 1 cut(s) 2444
NsiI ATGCAT 2 cut(s) 802, 1473
NspI RCATGY 2 cut(s) 991, 1248
NspV TTCGAA 2 cut(s) 1480, 1875
PaeI GCATGC 1 cut(s) 991
PagI TCATGA 1 cut(s) 1978
PciSI GCTCTTC 1 cut(s) 1686
PctI GAATGC 1 cut(s) 1137
PdmI GAANNNNTTC 3 cut(s) 198, 2202, 2355
PfeI GAWTC 8 cut(s) 194, 1353, 1656, 2024, 2305, 2351, 2521, 2725
PfoI TCCNGGA 1 cut(s) 2565
PinAI ACCGGT 1 cut(s) 809
PkrI GCNGC 5 cut(s) 340, 512, 748, 846, 1678
PleI GAGTC 3 cut(s) 562, 1258, 2763
PmeI GTTTAAAC 1 cut(s) 1791
PpsI GAGTC 3 cut(s) 562, 1258, 2763
Psp6I CCWGG 4 cut(s) 495, 693, 1181, 2565
PspFI CCCAGC 1 cut(s) 2432
PspGI CCWGG 4 cut(s) 495, 693, 1181, 2565
PspN4I GGNNCC 6 cut(s) 63, 478, 2231, 2533, 2571, 2625
PspPI GGNCC 2 cut(s) 970, 1897
PstI CTGCAG 2 cut(s) 340, 846
PsuI RGATCY 3 cut(s) 61, 332, 1837
RsaI GTAC 5 cut(s) 19, 525, 1236, 1311, 2625
RsaNI GTAC 5 cut(s) 18, 524, 1235, 1310, 2624
RseI CAYNNNNRTG 5 cut(s) 864, 876, 1168, 1343, 1746
SalI GTCGAC 1 cut(s) 2579
SapI GCTCTTC 1 cut(s) 1686
SatI GCNGC 5 cut(s) 339, 511, 747, 845, 1677
Sau96I GGNCC 2 cut(s) 970, 1897
SchI GAGTC 3 cut(s) 563, 1258, 2764
ScrFI CCNGG 4 cut(s) 497, 695, 1183, 2567
SduI GDGCHC 1 cut(s) 817
SfaNI GCATC 3 cut(s) 253, 908, 1458
SfcI CTRYAG 3 cut(s) 336, 842, 1851
SfuI TTCGAA 2 cut(s) 1480, 1875
SinI GGWCC 1 cut(s) 970
SmiMI CAYNNNNRTG 5 cut(s) 864, 876, 1168, 1343, 1746
SmlI CTYRAG 6 cut(s) 1402, 1412, 1616, 1892, 2510, 2720
SmoI CTYRAG 6 cut(s) 1402, 1412, 1616, 1892, 2510, 2720
SphI GCATGC 1 cut(s) 991
SsiI CCGC 2 cut(s) 445, 2788
SspMI CTAG 8 cut(s) 368, 1293, 1307, 1521, 1835, 2046, 2153, 2837
StyD4I CCNGG 4 cut(s) 495, 693, 1181, 2565
StyI CCWWGG 6 cut(s) 151, 558, 1292, 1314, 1503, 1605
TaaI ACNGT 6 cut(s) 79, 360, 1551, 1872, 2119, 2658
TaiI ACGT 2 cut(s) 1063, 2323
TaqI TCGA 5 cut(s) 270, 1480, 1875, 2098, 2580
TaqII GACCGA 1 cut(s) 836
TfiI GAWTC 8 cut(s) 194, 1353, 1656, 2024, 2305, 2351, 2521, 2725
TscAI CASTG 1 cut(s) 2619
TseFI GTSAC 1 cut(s) 2444
TseI GCWGC 5 cut(s) 338, 510, 746, 844, 1676
Tsp45I GTSAC 1 cut(s) 2444
TspGWI ACGGA 2 cut(s) 563, 1160
TspRI CASTG 1 cut(s) 2619
Vha464I CTTAAG 2 cut(s) 1402, 1892
VpaK11BI GGWCC 1 cut(s) 970
XapI RAATTY 6 cut(s) 326, 1391, 1830, 2072, 2561, 2639
XbaI TCTAGA 1 cut(s) 1834
XceI RCATGY 2 cut(s) 991, 1248
XcmI CCANNNNNNNNNTGG 2 cut(s) 631, 1204
XmaJI CCTAGG 1 cut(s) 1292
XmiI GTMKAC 1 cut(s) 2580
XmnI GAANNNNTTC 3 cut(s) 198, 2202, 2355
XspI CTAG 8 cut(s) 368, 1293, 1307, 1521, 1835, 2046, 2153, 2837
Zsp2I ATGCAT 2 cut(s) 802, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.