Rroxscaffold_6G00402260

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009232
Physical Location & Seq
Forward (+)
24610751 .. 24615050
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402260.1

Sequence Viewer

Length: 4101 bp
ATGGTCTTAGGTACAAAGGAGGAACATAAAAAGAAGATATTGGAGCTGTTGAAGGATCCCAATGTGTCAACAGTAGTTCTTGATGGGAAGCGTGGAGTAGGGAAAACGAGGATAGCAAAAGAGATAACCATGGATGAAGATTTCCATGACACTCTCTGGCTGTATGTGAATAACAATTATGACACAGAATCTCTTCACAAAAACATTGCTCGCCGGCTATCTCTTTTCTCTATTTATGAGGAATGGGAAGATGATGATGCTGTCGAGGAGGAGCAGAGCTTGGAAAAGTTGAAGCCGAGAATATCTGTGAAGCTTGAAGAATTCAGATCTGCAGCAGATGAAAAAAATAAACTGTTTCTTCTAGTTCTGGATGATGTTCCTCATCAGAAGGATGTAGAGGAGATTGTGGAAGAAGTTAACAACCTGCTGACAAATGACCGCTCATCTTTCAAGGTTTTAATCACCAGAGGCACCACACTCGGGATGAACCAGGAAATTAAGAGTTGCAATACAAAAGTACACCAGATAAAACCATTGTCCACGGATGAGTCCTTGGCTTTATTGCGTGAAAGAACTAAGAAAGAAGTTTTTGATCGTCCAAGCTTTGAAAAACTATCCACAGCTATTGTGGAGATGAGTAAGGGTATTCCTACCATACTTATCACCATAGCAAAAGCAATAAATTACCTGGGAGATGAATGTTCTGGCGTTTGGTCGTTGGAAAATGTTTTGGAAGAAGCAGCTTATGATGGGGAAACGGCTATAAATCTGCTACTTCGCTCTTGGTGTAATGCATTGCCAACCGGTGCGCCGACCGACTGCTTTTGGCATACCCTGCAGCTTCTCAACAAACATGGTGGTGTTCATTACAATGAGCTGATCACACAATGGATAATGGAAGGATATTTTGGATGCAACAACCAGATTGGGAAGGTCTATGAGCAAGGACATAAGGTTCTGATGGACCTTATAGATTGGGGCATGCTGACAAAACAAGAGGATAATATGGTTGTCATGGGAGGAATAATTTTGAGTATAACTGGTCATTGCCGACGTGAATTGAATGAGACTGCAAGGCTTGGATTGGCTAATGTGTTTGAAGATGAAAATTGGAAAGGTCTTGGAATGCTTGCACATACGGATCGTATGATAAAATCACCATCCTGTCATAAGACCTGGGAGAAAGTTTCCACACTTCTGATGGATGGGCATTGCATTGATAGGGAAGTACCTGTAACATGCTTTCAACGCATGGAGACTCTCCAAGTTCTCATGTTAATCAAGCCTAGGTTCTCTCTGCCAGTACCCTTGGTTGGCAGAAATAACCTTTCCATTCTTGTGCTTAGATTCTGTGATCGCTTGGAGAAGATTGATCCTATCCGAGAATTTCAAAGCTTAAGTGTTCTTGAGATATCTGGTGCTACTTTGTTGGAGATGATCCCCAATGACTTTTTTACAAAGATGCATAAGCTTCGAAGCGTTAACCTTTCTGAAGCCAAGGTCAAATCACTACCTAGTTCTCTTTTTGAAAGAAGTGAACTACGGTGGCTCATCCTTAGAGGATGTTCTCAATTGAAAACACTTCCAAGCTTAAAATCCTTGGGAAAGCTCAAGGTGCTTGATCTTTCTGGTGCTACCTCTTTCAGCAGATTCCGAGACAAAACCTTTGCAGCTCTTCAGAAACTTCATACACTTAATCTTTCTAATTCTCAAATTACTCGCTTACCTTTTCTTCATGGTAATGATGAGCTTAATCAGCTCCTATTAGGTGGCTGTGCAAGTTTAAACAGACTTCCAACCTTGAATACAATACCTCGGCTTCAAATTCTAGATCTTTCTGGTGCTACAGGCTTAAAAGAAATACAGTTCGAACCTTTAGATAGCCTTAAGGCCCTTGATTTATCTGGAACTCATATTAAAAGGCAACCTTCCAGTTTCATCCACCTTTCTGATCTCTCCTTACGAGAGTGTCATGAGCTTGTAAGACTTCCACTCACAAAAGAAATCAAATATCTTGAATCACTTGACCTTTCTGGTTCTAGCACTACGGTTGAAATTGAAGACAAATTCTTCCAGCATATGAGGTTTCTTCGAGTTCTCAACTTTTCAACAGTTAAGATAAAAGAACTACCTTCCCTGTCCAACCTAGCTAACCTCTGTCGACTTTTGCTAATGGATTGTTTATGTTTAGAAAAGCTTCCAGAGATGGAAGGACTTAAAAGGCTCCAAGAGCTTAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCAAACCTGAACTCCCTTGAAAAACTGGAGATTCTTGATATTTCACGTTGCAGGGTGAAACAAATACAAGGGGAATCCTTTCAAAAGATGTCCCATATTCGGATACTCAACCTCTCAGACACTATGATTAAGTTCCTACCATCCATTTGCAAACCCAGCAACCTCTGTCACCTCATCCTCAGAAATTGCAAAAACTTAAACAAGCTTCCACCTCTTGATCATTTGTCAAAACTTGAGGAATTGAATCTGTGTGGTGCCAGTTCTTTAGTTGACTTTACATCTGAATTCCTGGAGCATATGGTCGACCTTCAAATTGTTGACTTATCTGAAATCCCACTGACAAAGGTACTATCCATCAGTTCCGAGTCTGCCTCCCTAAATACTTTTAGCAACCTCCGCCAGCTCTTGCTAAAAGATTGTTTAAAGATAGAAGAGTTGCAGTTTCTAGAGTCGCTCAGTCGGCTGGAGGTTCTTGACCTGTCAGGAACCAGATTGAAGCAATTTCCTTATGAGATATCAGAGCTTACTCATTTAAAGCAGCTTTATCTTCCTGACTTGAGGGGTATGCGTGATCTTGACTTGGGAAAGATAAAGCGTTTGCCACAAAAGGTCAACTGGGATGAATGTCACATCTTTGAGTGTGCTGATATTTACATGGGCAGTGACAAGCCTTCCCTATCTACGAAGGAAGGCGAAGATGGAGGTATCAATCACTGCAAAGATGAATTTATCTTCAGAGATGTGTACTTCCACACTAGACATTTTTATCCTAAAGAGCACGACAGGTCTTTGGAAATCTATGGATCGTATAGTCTCCCTAAGGGTTTTGAAAGTGTCCTCAAGTGTGCCGAATGCATATCTTTGGTTGACAATGACCTTATACATAGCCTATCTGATTTAGGTTCTGATAATGTGAGGGCAATGAAAGGCTGTTGGTTAGAGAGATGTAGTAAAGTACAATACATTCTTTCTAATGAAGAGGCAGGTGTCACACTGGGCAGAAGTTTAGAGAGTCTATGGATTTCAAACCTTCCAATTTTGAGCAGTCTATACAATGGGAGTGTGCAATCAGTTGTCTTTCAGAACCTTAAACAATTGTATCTGGATTGTTGTCCAGGCATCGTAAATGTCTTTCCATCCTCCCAGCTTCCAGAAAACCTAGAAACTCTACATGTTCAGTTTTGTGATCAGTTGCAAACTTTGTTCGAATGTGATTCACAATCTAGTTGTGCAATGCAAAGGTTGAGGAAATTGTTTCTACTGGATCTACCAGAGTTGAGGAGCATTGGGATAAAATTGCCAGAAGACCTTGAAATTCTACATGTTCAGTCTTGTGATAAGTTGCAAAATTTGTTCGAGTGTGATTCACAATCTAGTTGTGCAATGCAAAGATTGAGGAAATTGTATCTACAGGAACTACCGGAGTTGAGGAGCATTGGGATAAAATTGCCAGAAGGCCTGGAAATTCTACATGTTCAGTCTTGTGATAAGTTGCAAACTTTGTTCGAGTGTGATTCACAATCTAGTTGTGCAATGCAAAGACTGAGGAAATTGTATCTACTGGAACTACCGGAGTTGAGAAGCATTGGGATAAAATTGCCAGAAGACCTGGAAATTCTACATGTTCAGTCTTGTGATAAGTTGCAAACTTTGTTTGAGTGTGATTCACAATCTAGTTGTGCAATGCAAAGATTGAGGAAATTGTATCTACTGGATCTACCAGAGTTGAGGAGCATTGGGATAAAATTGCCAGCTGAACTGACTTCTGTGGTCATCAAATGCCCCAAGTTGCTGAAGCTCCAGTCGGACGCGACCGACCAGGTCTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

1366

Amino Acids

155.54

Weight (kDa)

5.83

Isoelectric Point (pI)

52.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 7 - 191 1.5e-12 NB-ARC domain
LRR_14 PF23598 454 - 571 7e-07 Leucine-rich repeat region
LRR_14 PF23598 693 - 838 8.7e-06 Leucine-rich repeat region
LRR_8 PF13855 764 - 819 2.1e-06 Leucine rich repeat
LRR_RPS2 PF23247 1113 - 1185 3.1e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1192 - 1258 3.3e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3242
Acc36I ACCTGC 2 cut(s) 432, 3242
AccB1I GGYRCC 2 cut(s) 470, 2522
AccBSI CCGCTC 1 cut(s) 441
AccI GTMKAC 2 cut(s) 2161, 2571
AccII CGCG 1 cut(s) 4079
AciI CCGC 2 cut(s) 439, 2665
AclWI GGATC 8 cut(s) 50, 63, 1149, 1367, 1432, 3079, 3540, 3990
AcuI CTGAAG 4 cut(s) 1512, 1661, 2986, 4082
AfaI GTAC 7 cut(s) 13, 519, 1230, 1305, 2616, 3014, 3225
AfiI CCNNNNNNNGG 3 cut(s) 480, 1313, 2367
AflII CTTAAG 2 cut(s) 1396, 1886
AflIII ACRYGT 4 cut(s) 3439, 3589, 3739, 3889
AgeI ACCGGT 1 cut(s) 803
AjiI CACGTC 1 cut(s) 1055
AjnI CCWGG 8 cut(s) 489, 687, 1175, 2556, 3382, 3726, 3876, 4086
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AloI GAACNNNNNNTCC 2 cut(s) 939, 971
Alw21I GWGCWC 1 cut(s) 3048
Alw26I GTCTC 5 cut(s) 1061, 1250, 1650, 3086, 4096
AlwI GGATC 8 cut(s) 50, 63, 1149, 1367, 1432, 3079, 3540, 3990
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 2 cut(s) 1890, 3724
ApeKI GCWGC 5 cut(s) 332, 740, 838, 1670, 2806
ArsI GACNNNNNNTTYG 2 cut(s) 1649, 1681
AsiGI ACCGGT 1 cut(s) 803
Asp700I GAANNNNTTC 3 cut(s) 192, 2196, 2346
AspA2I CCTAGG 1 cut(s) 1286
AspLEI GCGC 1 cut(s) 811
AspS9I GGNCC 2 cut(s) 964, 1891
AsuHPI GGTGA 5 cut(s) 454, 655, 1149, 2335, 2429
AsuII TTCGAA 3 cut(s) 1474, 1869, 3474
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 964
AvrII CCTAGG 1 cut(s) 1286
AxyI CCTNAGG 1 cut(s) 3087
BaeI ACNNNNGTAYC 2 cut(s) 3350, 3383
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 2 cut(s) 470, 2522
BbsI GAAGAC 3 cut(s) 2067, 3579, 3879
Bbv12I GWGCWC 1 cut(s) 3048
BbvI GCAGC 5 cut(s) 344, 752, 850, 1682, 2818
BceAI ACGGC 1 cut(s) 774
BcgI CGANNNNNNTGC 4 cut(s) 460, 494, 1454, 1488
BciT130I CCWGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
BciVI GTATCC 1 cut(s) 2364
BclI TGATCA 3 cut(s) 879, 2485, 3454
BcoDI GTCTC 5 cut(s) 1061, 1250, 1650, 3086, 4096
BfmI CTRYAG 4 cut(s) 330, 836, 1845, 3677
BfrI CTTAAG 2 cut(s) 1396, 1886
BfuAI ACCTGC 2 cut(s) 432, 3242
BfuI GTATCC 1 cut(s) 2364
BglII AGATCT 2 cut(s) 326, 1831
BisI GCNGC 5 cut(s) 333, 741, 839, 1671, 2807
BlnI CCTAGG 1 cut(s) 1286
BlsI GCNGC 5 cut(s) 334, 742, 840, 1672, 2808
Bme1390I CCNGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
Bme18I GGWCC 1 cut(s) 964
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 1055
BmgT120I GGNCC 2 cut(s) 964, 1891
BmiI GGNNCC 5 cut(s) 57, 472, 2225, 2524, 2755
BmrFI CCNGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
BmrI ACTGGG 2 cut(s) 2893, 3272
BmsI GCATC 4 cut(s) 247, 902, 1452, 3396
BmuI ACTGGG 2 cut(s) 2893, 3272
BpiI GAAGAC 3 cut(s) 2067, 3579, 3879
BplI GAGNNNNNCTC 2 cut(s) 2624, 2656
BpmI CTGGAG 4 cut(s) 2315, 2579, 2753, 4052
Bpu14I TTCGAA 3 cut(s) 1474, 1869, 3474
BpuEI CTTGAG 5 cut(s) 1427, 1595, 2522, 2845, 3092
BsaI GGTCTC 1 cut(s) 4096
BsaWI WCCGGW 3 cut(s) 803, 3688, 3838
BsaXI ACNNNNNCTCC 3 cut(s) 2264, 2294, 4076
Bsc4I CCNNNNNNNGG 3 cut(s) 480, 1313, 2367
Bse118I RCCGGY 2 cut(s) 213, 803
Bse21I CCTNAGG 1 cut(s) 3087
Bse3DI GCAATG 9 cut(s) 204, 794, 1045, 1210, 3195, 3507, 3657, 3807, 3957
BseBI CCWGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
BseLI CCNNNNNNNGG 3 cut(s) 480, 1313, 2367
BseMI GCAATG 9 cut(s) 204, 794, 1045, 1210, 3195, 3507, 3657, 3807, 3957
BseMII CTCAG 4 cut(s) 2397, 2461, 2737, 3803
BseRI GAGGAG 6 cut(s) 281, 284, 413, 3562, 3712, 4012
BseXI GCAGC 5 cut(s) 344, 752, 850, 1682, 2818
BseYI CCCAGC 2 cut(s) 2423, 3411
Bsh1236I CGCG 1 cut(s) 4079
Bsh1285I CGRYCG 2 cut(s) 816, 4083
BshFI GGCC 2 cut(s) 1892, 3726
BshNI GGYRCC 2 cut(s) 470, 2522
BshTI ACCGGT 1 cut(s) 803
BsiEI CGRYCG 2 cut(s) 816, 4083
BsiHKAI GWGCWC 1 cut(s) 3048
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 4 cut(s) 214, 804, 3689, 3839
BslFI GGGAC 1 cut(s) 2344
BslI CCNNNNNNNGG 3 cut(s) 480, 1313, 2367
BsmAI GTCTC 5 cut(s) 1061, 1250, 1650, 3086, 4096
BsmFI GGGAC 1 cut(s) 2344
BsmI GAATGC 2 cut(s) 1131, 3125
BsnI GGCC 2 cut(s) 1892, 3726
Bso31I GGTCTC 1 cut(s) 4096
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 3 cut(s) 1474, 1869, 3474
Bsp1286I GDGCHC 1 cut(s) 3048
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 2 cut(s) 439, 2665
BspANI GGCC 2 cut(s) 1892, 3726
BspCNI CTCAG 4 cut(s) 2396, 2460, 2736, 3804
BspFNI CGCG 1 cut(s) 4079
BspHI TCATGA 1 cut(s) 1972
BspLI GGNNCC 5 cut(s) 57, 472, 2225, 2524, 2755
BspMAI CTGCAG 2 cut(s) 334, 840
BspMI ACCTGC 2 cut(s) 432, 3242
BspPI GGATC 8 cut(s) 50, 63, 1149, 1367, 1432, 3079, 3540, 3990
BspQI GCTCTTC 1 cut(s) 1680
BspT104I TTCGAA 3 cut(s) 1474, 1869, 3474
BspT107I GGYRCC 2 cut(s) 470, 2522
BspTI CTTAAG 2 cut(s) 1396, 1886
BspTNI GGTCTC 1 cut(s) 4096
BsrBI CCGCTC 1 cut(s) 441
BsrDI GCAATG 9 cut(s) 204, 794, 1045, 1210, 3195, 3507, 3657, 3807, 3957
BsrFI RCCGGY 2 cut(s) 213, 803
BssAI RCCGGY 2 cut(s) 213, 803
BssT1I CCWWGG 6 cut(s) 129, 552, 1286, 1308, 1497, 1599
Bst2UI CCWGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
Bst4CI ACNGT 6 cut(s) 73, 354, 1545, 1866, 2050, 2113
Bst6I CTCTTC 4 cut(s) 198, 1680, 2694, 3240
BstAFI CTTAAG 2 cut(s) 1396, 1886
BstAPI GCANNNNNTGC 1 cut(s) 835
BstBI TTCGAA 3 cut(s) 1474, 1869, 3474
BstC8I GCNNGC 6 cut(s) 211, 215, 983, 1131, 2669, 4020
BstDEI CTNAG 9 cut(s) 7, 576, 1343, 1556, 2383, 2447, 2723, 3087, 3812
BstDSI CCRYGG 2 cut(s) 129, 540
BstFNI CGCG 1 cut(s) 4079
BstHHI GCGC 1 cut(s) 811
BstMAI GTCTC 5 cut(s) 1061, 1250, 1650, 3086, 4096
BstMCI CGRYCG 2 cut(s) 816, 4083
BstMWI GCNNNNNNNGC 8 cut(s) 835, 1248, 1615, 1756, 2230, 2424, 2664, 2728
BstNI CCWGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
BstNSI RCATGY 6 cut(s) 985, 1242, 3443, 3593, 3743, 3893
BstSCI CCNGG 8 cut(s) 489, 687, 1175, 2556, 3382, 3726, 3876, 4086
BstSFI CTRYAG 4 cut(s) 330, 836, 1845, 3677
BstUI CGCG 1 cut(s) 4079
BstV1I GCAGC 5 cut(s) 344, 752, 850, 1682, 2818
BstV2I GAAGAC 3 cut(s) 2067, 3579, 3879
BstX2I RGATCY 5 cut(s) 55, 326, 1831, 3532, 3982
BstYI RGATCY 5 cut(s) 55, 326, 1831, 3532, 3982
Bsu36I CCTNAGG 1 cut(s) 3087
BsuI GTATCC 1 cut(s) 2364
BsuRI GGCC 2 cut(s) 1892, 3726
BtgI CCRYGG 2 cut(s) 129, 540
BtrI CACGTC 1 cut(s) 1055
BtsI GCAGTG 2 cut(s) 2935, 2980
BtsIMutI CAGTG 4 cut(s) 2603, 2935, 2980, 3260
BveI ACCTGC 2 cut(s) 432, 3242
Cac8I GCNNGC 6 cut(s) 211, 215, 983, 1131, 2669, 4020
CciI TCATGA 1 cut(s) 1972
CfoI GCGC 1 cut(s) 811
Cfr10I RCCGGY 2 cut(s) 213, 803
Cfr13I GGNCC 2 cut(s) 964, 1891
CseI GACGC 1 cut(s) 4085
CsiI ACCWGGT 1 cut(s) 4086
Csp6I GTAC 7 cut(s) 12, 518, 1229, 1304, 2615, 3013, 3224
CspAI ACCGGT 1 cut(s) 803
CspCI CAANNNNNGTGG 4 cut(s) 607, 642, 838, 873
CviQI GTAC 7 cut(s) 12, 518, 1229, 1304, 2615, 3013, 3224
DdeI CTNAG 9 cut(s) 7, 576, 1343, 1556, 2383, 2447, 2723, 3087, 3812
DraI TTTAAA 3 cut(s) 1785, 2691, 2802
Eam1104I CTCTTC 4 cut(s) 198, 1680, 2694, 3240
EarI CTCTTC 4 cut(s) 198, 1680, 2694, 3240
EciI GGCGGA 1 cut(s) 2654
Eco130I CCWWGG 6 cut(s) 129, 552, 1286, 1308, 1497, 1599
Eco147I AGGCCT 1 cut(s) 3726
Eco31I GGTCTC 1 cut(s) 4096
Eco32I GATATC 2 cut(s) 1413, 2784
Eco47I GGWCC 1 cut(s) 964
Eco57I CTGAAG 4 cut(s) 1512, 1661, 2986, 4082
Eco81I CCTNAGG 1 cut(s) 3087
Eco88I CYCGRG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 1891
EcoRI GAATTC 2 cut(s) 320, 2552
EcoRII CCWGG 8 cut(s) 489, 687, 1175, 2556, 3382, 3726, 3876, 4086
EcoRV GATATC 2 cut(s) 1413, 2784
EcoT14I CCWWGG 6 cut(s) 129, 552, 1286, 1308, 1497, 1599
EcoT22I ATGCAT 3 cut(s) 796, 1467, 3125
ErhI CCWWGG 6 cut(s) 129, 552, 1286, 1308, 1497, 1599
FalI AAGNNNNNCTT 2 cut(s) 1912, 1944
FaqI GGGAC 1 cut(s) 2344
FauNDI CATATG 2 cut(s) 2079, 2565
FbaI TGATCA 3 cut(s) 879, 2485, 3454
FblI GTMKAC 2 cut(s) 2161, 2571
Fnu4HI GCNGC 5 cut(s) 333, 741, 839, 1671, 2807
Fsp4HI GCNGC 5 cut(s) 333, 741, 839, 1671, 2807
GlaI GCGC 1 cut(s) 810
GluI GCNGC 5 cut(s) 333, 741, 839, 1671, 2807
GsaI CCCAGC 2 cut(s) 2427, 3415
GsuI CTGGAG 4 cut(s) 2315, 2579, 2753, 4052
HaeIII GGCC 2 cut(s) 1892, 3726
HapII CCGG 4 cut(s) 214, 804, 3689, 3839
HgaI GACGC 1 cut(s) 4085
HhaI GCGC 1 cut(s) 811
Hin6I GCGC 1 cut(s) 809
HinP1I GCGC 1 cut(s) 809
HincII GTYRAC 9 cut(s) 69, 418, 1483, 2162, 2539, 2572, 2587, 2881, 3136
HindII GTYRAC 9 cut(s) 69, 418, 1483, 2162, 2539, 2572, 2587, 2881, 3136
HindIII AAGCTT 7 cut(s) 311, 601, 1393, 1469, 1588, 2195, 2471
HpaI GTTAAC 2 cut(s) 418, 1483
HpaII CCGG 4 cut(s) 214, 804, 3689, 3839
HphI GGTGA 5 cut(s) 454, 655, 1149, 2335, 2429
Hpy99I CGWCG 1 cut(s) 1056
HpyCH4III ACNGT 6 cut(s) 73, 354, 1545, 1866, 2050, 2113
HpyCH4IV ACGT 2 cut(s) 1054, 2314
HpyF10VI GCNNNNNNNGC 8 cut(s) 835, 1248, 1615, 1756, 2230, 2424, 2664, 2728
HpyF3I CTNAG 9 cut(s) 7, 576, 1343, 1556, 2383, 2447, 2723, 3087, 3812
HpySE526I ACGT 2 cut(s) 1054, 2314
HspAI GCGC 1 cut(s) 809
KroI GCCGGC 1 cut(s) 213
KroNI GCCGGC 1 cut(s) 215
Ksp22I TGATCA 3 cut(s) 879, 2485, 3454
KspAI GTTAAC 2 cut(s) 418, 1483
LguI GCTCTTC 1 cut(s) 1680
LmnI GCTCC 9 cut(s) 43, 271, 1764, 2229, 2560, 3549, 3699, 3999, 4071
Lsp1109I GCAGC 5 cut(s) 344, 752, 850, 1682, 2818
LweI GCATC 4 cut(s) 247, 902, 1452, 3396
MabI ACCWGGT 1 cut(s) 4086
MaeII ACGT 2 cut(s) 1054, 2314
MaeIII GTNAC 5 cut(s) 1234, 2435, 2894, 2930, 3256
MbiI CCGCTC 1 cut(s) 441
MfeI CAATTG 2 cut(s) 1571, 3362
MflI RGATCY 5 cut(s) 55, 326, 1831, 3532, 3982
MhlI GDGCHC 1 cut(s) 3048
MlyI GAGTC 5 cut(s) 557, 1252, 2642, 2726, 3289
MmeI TCCRAC 5 cut(s) 699, 1410, 1820, 2166, 4053
Mph1103I ATGCAT 3 cut(s) 796, 1467, 3125
MroNI GCCGGC 1 cut(s) 213
MroXI GAANNNNTTC 3 cut(s) 192, 2196, 2346
MslI CAYNNNNRTG 4 cut(s) 858, 870, 1337, 1740
MspA1I CMGCKG 1 cut(s) 4022
MspCI CTTAAG 2 cut(s) 1396, 1886
MspI CCGG 4 cut(s) 214, 804, 3689, 3839
MspR9I CCNGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
MssI GTTTAAAC 1 cut(s) 1785
MunI CAATTG 2 cut(s) 1571, 3362
Mva1269I GAATGC 2 cut(s) 1131, 3125
MvaI CCWGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
MvnI CGCG 1 cut(s) 4079
MwoI GCNNNNNNNGC 8 cut(s) 835, 1248, 1615, 1756, 2230, 2424, 2664, 2728
NaeI GCCGGC 1 cut(s) 215
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 2079, 2565
NgoMIV GCCGGC 1 cut(s) 213
NlaIV GGNNCC 5 cut(s) 57, 472, 2225, 2524, 2755
NmeAIII GCCGAG 2 cut(s) 321, 1795
NmuCI GTSAC 4 cut(s) 2435, 2894, 2930, 3256
NsiI ATGCAT 3 cut(s) 796, 1467, 3125
NspI RCATGY 6 cut(s) 985, 1242, 3443, 3593, 3743, 3893
NspV TTCGAA 3 cut(s) 1474, 1869, 3474
PaeI GCATGC 1 cut(s) 985
PagI TCATGA 1 cut(s) 1972
PaqCI CACCTGC 1 cut(s) 3242
PceI AGGCCT 1 cut(s) 3726
PciI ACATGT 4 cut(s) 3439, 3589, 3739, 3889
PciSI GCTCTTC 1 cut(s) 1680
PctI GAATGC 2 cut(s) 1131, 3125
PdiI GCCGGC 1 cut(s) 215
PdmI GAANNNNTTC 3 cut(s) 192, 2196, 2346
PflFI GACNNNGTC 1 cut(s) 4088
PfoI TCCNGGA 1 cut(s) 2556
PinAI ACCGGT 1 cut(s) 803
PkrI GCNGC 5 cut(s) 334, 742, 840, 1672, 2808
PleI GAGTC 5 cut(s) 556, 1252, 2641, 2725, 3288
PmeI GTTTAAAC 1 cut(s) 1785
PpsI GAGTC 5 cut(s) 556, 1252, 2641, 2725, 3288
PscI ACATGT 4 cut(s) 3439, 3589, 3739, 3889
Psp6I CCWGG 8 cut(s) 489, 687, 1175, 2556, 3382, 3726, 3876, 4086
PspFI CCCAGC 2 cut(s) 2423, 3411
PspGI CCWGG 8 cut(s) 489, 687, 1175, 2556, 3382, 3726, 3876, 4086
PspN4I GGNNCC 5 cut(s) 57, 472, 2225, 2524, 2755
PspPI GGNCC 2 cut(s) 964, 1891
PstI CTGCAG 2 cut(s) 334, 840
PsuI RGATCY 5 cut(s) 55, 326, 1831, 3532, 3982
PsyI GACNNNGTC 1 cut(s) 4088
PvuII CAGCTG 1 cut(s) 4022
RsaI GTAC 7 cut(s) 13, 519, 1230, 1305, 2616, 3014, 3225
RsaNI GTAC 7 cut(s) 12, 518, 1229, 1304, 2615, 3013, 3224
RseI CAYNNNNRTG 4 cut(s) 858, 870, 1337, 1740
SalI GTCGAC 2 cut(s) 2160, 2570
SapI GCTCTTC 1 cut(s) 1680
SatI GCNGC 5 cut(s) 333, 741, 839, 1671, 2807
Sau96I GGNCC 2 cut(s) 964, 1891
SchI GAGTC 5 cut(s) 557, 1252, 2642, 2726, 3289
ScrFI CCNGG 8 cut(s) 491, 689, 1177, 2558, 3384, 3728, 3878, 4088
SduI GDGCHC 1 cut(s) 3048
SexAI ACCWGGT 1 cut(s) 4086
SfaNI GCATC 4 cut(s) 247, 902, 1452, 3396
SfcI CTRYAG 4 cut(s) 330, 836, 1845, 3677
SfuI TTCGAA 3 cut(s) 1474, 1869, 3474
SinI GGWCC 1 cut(s) 964
SmiMI CAYNNNNRTG 4 cut(s) 858, 870, 1337, 1740
SmlI CTYRAG 7 cut(s) 1396, 1406, 1610, 1886, 2501, 2824, 3107
SmoI CTYRAG 7 cut(s) 1396, 1406, 1610, 1886, 2501, 2824, 3107
SphI GCATGC 1 cut(s) 985
SseBI AGGCCT 1 cut(s) 3726
SsiI CCGC 2 cut(s) 439, 2665
StuI AGGCCT 1 cut(s) 3726
StyD4I CCNGG 8 cut(s) 489, 687, 1175, 2556, 3382, 3726, 3876, 4086
StyI CCWWGG 6 cut(s) 129, 552, 1286, 1308, 1497, 1599
TaaI ACNGT 6 cut(s) 73, 354, 1545, 1866, 2050, 2113
TaiI ACGT 2 cut(s) 1057, 2317
TaqI TCGA 9 cut(s) 264, 1474, 1869, 2092, 2161, 2571, 3474, 3624, 3774
TaqII GACCGA 2 cut(s) 830, 4097
TatI WGTACW 3 cut(s) 517, 3012, 3223
TscAI CASTG 4 cut(s) 2610, 2935, 2987, 3267
TseFI GTSAC 4 cut(s) 2435, 2894, 2930, 3256
TseI GCWGC 5 cut(s) 332, 740, 838, 1670, 2806
Tsp45I GTSAC 4 cut(s) 2435, 2894, 2930, 3256
TspGWI ACGGA 2 cut(s) 557, 1154
TspRI CASTG 4 cut(s) 2610, 2935, 2987, 3267
Tth111I GACNNNGTC 1 cut(s) 4088
Vha464I CTTAAG 2 cut(s) 1396, 1886
VpaK11BI GGWCC 1 cut(s) 964
XbaI TCTAGA 2 cut(s) 1828, 2713
XceI RCATGY 6 cut(s) 985, 1242, 3443, 3593, 3743, 3893
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 1198
XmaJI CCTAGG 1 cut(s) 1286
XmiI GTMKAC 2 cut(s) 2161, 2571
XmnI GAANNNNTTC 3 cut(s) 192, 2196, 2346
Zsp2I ATGCAT 3 cut(s) 796, 1467, 3125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.