Rw3G020230

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009232
Physical Location & Seq
Reverse (-)
23640688 .. 23651735
11048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020230.1

Sequence Viewer

Length: 3795 bp
ATGGCCTTAGGTACAAAGGAGGAACATATAAAGAAGATATTGGAGCTGTTGAAGGATCCCTATGTGCCAACAGTAGTTCTTGATGGGAAGCGTGGAGTAGGGAAAACGTGGATAGCAAAAGAGATAATCAAGGATAAAGATTTAATCCATGACACTCTCTGGCTGTATCTATCTCTATTCTCTATTTATGAGGAATGGGAAGATGATGATGCTGCTGAGGAGGAACAGAGATTGGAGAAGTTGATGCCGAGAATATCTGAGAAGCTTGAAGAGTTCAGATCTGCAGCACATGAAGAAAAGAAACTATTTCTTCTTATTCTGGATGATCTTCCTCATCAGAAGGATGTAGAGGAGATTGTGAACGAAGTTAACAACCTGCTGACTTCAAATGAATCTTTCAAGGTTTTAATCACCAGAGGCGAAAGCGATGGAAGAGGCACCACGCTGGGGATGAACCAGGAAATTAAGAGCTGCAATACAACAGTATACCCGATAGATCCATTGTCCACTGATGAGTCCTCAGCTTTATTGCATGAAAAAATCAAGAAAGAAGTATTTGATTGTCCAGGCTTTGGAAAACTATCCAACGCTATTGTGGAGATGAGTAAGGGAATTCCTACTATACTTATCACGACAGCAAAAGCAATAAATTACTTGGCAGATAAAGGTTCCGAGGTTTGGTCGTTGGAAAATATTTTGGAAGAAGCGGCTTATGATGGGGAAACTGCTATAAATTTGCTACTTCGCTCTTGGCGTAATGCATTGCCAACCGATTCTCTGACAGACTGCTTTTGGCATACCATGCAGCTTCTAAACAAACATGGTGGTGTTCATTACAATGAGTTGATCACACAATGGATAATGGAAGGATATTTTGGATGCAACAATCAGATTGGGAAGGTCTATGAGCAAGGACATAAGGTCCTGATGGACCTTATAGATTGGGGCATGCTGAGAAAACAAGAGGATAACATGGTTGTAATGGGAGGAATAATTTTGTGTATAACTGGTCATTATCGACGTGAATTGAATGCGACTGCAAGGCTCGGAATGGCTAATGTGTTTGAAGATGAAAATTGGAAAGGTCTTGGAATGCTTGCACATACGGATCGTATGATAAAATCACCATCCAGTCATAAGACCTGGGAGAAAGTTTCCACACTTCTGATGGATGGCCAGTGCATCGATAGGGAAGTACCTGTAACATGCTTTCAACGCATGGAGACTCTCCAAGTTCTCATGTTAATCAAGCCTAGGTTCACATCTCTGCCAGTATCCTTAGTTGACATAAAGAAACTTTCCATTCTTGTGCTCAGATTCTGTGATCGCTTGGAGAAGATTGATCCTATCCAAGAATTTCAAAGCTTAAGTGTTCTTGAGATATCTGGTGCTACTTCGTTGAAGATGATCCCTGATAACTTTTTTGAAACGATGAGTAACCTTCGGAGCCTTAACCTTTCTGAAGCCAATGTCAAATCACTACCTAGTTCTCTTTTCAACAGAAGTGAACTACGGTGGCTCATTCTTAGAGGATGTTCTCAATTGAAAACACTTCCAAGCTTAAAATCCTTGGGAAAGCTCAAGGTGCTTGATCTTTCTGGTGCTACCTCTTTCAGTAGATTCCGAGACAAAACCTTTGCAGCTCTTCAGAAACTTCATACACTTAATCTTTCTAATTCCCAAATTACTCGCTTACCCTTTCTTCATGGTAATGATGAGCTTAATCAGCTCCTATTAGGTGGTTGTGCAAGTTTAAACAGACTTCCAACCTTGAACACAATACCTCGGCTTCAAATTCTTGATCTTTCTGGTGCTACAGGCTTAAAAGAAATACAGTTTGAACCTTTGGATACCCTTAAGGCCCTTGATTTATCTGGAACTCTTATTAAAAGGCAACCTTCCAGTTTCAGCCACCTTTCTGATCTCTCCTTACGGGAGTGTCGTGAGCTTGTAAAACTTCCACTCACAAAAGAAATCAAATATCTTGAATCACTTGACCTTTCTGGTTCTAGCACCATCGTTGAAATTGAAGACAAATTCTTCCAGCAAATGAGGTTTCTTCGAGTTCTGAACTTTTCAACAGTTAAGATAAAAGAACTACCTTCCCTGTCCAACCTAGCTAACCTCTGTCGACTTTTGCTAATGGATTGTTCACGTTTAGAAAAGCTTCCAGAGATGGAAGGACTTAAAAGGCTCCAAGAGCTTAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCAAACCTGAACTCCCTTGAAAAACTGGAGATTCTTGATATTTCAGGCTGCAGGGTTTTGAAACAAATACAAGAGGAATCCTTTCAAAAGATGTTCCATATTCGGATACTCAACCTCTCAGACACTATGATTGAGTTCCTTCCATCCATTTGCAAACCCAGCAACCTCTGTCACCTCATCCTCAGAAATTGCAAACACTTAAAAAAGCTTCCACCTCTTGAGCATTTGTCAAAACTTGAGGAACTGAATCTGTGTGGTGCCAGTTCTTTAGTTGACTTTACATCTGAATTCCTGGAGCATATGGTCTACCTTCAAATTGTTGACTTATCTGAAATCCCATTGACAACGGTACCATCCATGTCCAAATTCATCAAACTCAGACAGTTCTCAGTAAAGGGTTGTTCATCCTTAAATGAAGTGCTACATCTTGAAGTACTAACACTTCTTGAGATTCTTGATCTTTCTGGAACAGCAGTTCCGAGTCTGCCTCCCCTAAACACTTTTAGCAACCTCCACCAGCTCTTGCTAAAAGATTGTTCACAAATAAAAGAGTTGCAGTTTCTAGAGTCGCTTAGTAGGCTGGAGGTTCTTGATCTGTCAGGAACCAGAATGAAGCAATTTCCTTATGAGATATCAGAGCTTACTCATTTAAAGCAGCTTTATCTGCCAGACTTGAGGGGTATGTCTGATCTTGACTTGGGAAAGATAAAGCGTTTGCCACAAGAGGTCAACTGGGATGAATGTGGCATCTTTGAGTGTGCTGATATTTACGTAGGCAGTGACAAGATTTCCCTATCTGTAAGTGGCTTTGAAATTTTTCACATTTTGGACAAACATCCAAAGTTTCGGGAAGAAAAGTTCAAAAAATTTCATTTCCATGTAAAGAATAAGGAAGGTGAAGATGGAGGTATCAAGTACTGCAAAGATGAATTTATCTTCAGAGATGTGTACTTCCACACTAGAAATTTTTATCCTAAAGAGCACGATAGGTCTTTGGAAATCTATGGATCATATAGTCTCCCTAAGGGTTTTGAAAGTGTCCTCAAGTGTGCCGAATGCTTATCTTTGGTTGACAATGACCTTATACGTAGCCTATCTGATTTAGGTTCTGACAATGTGAAGACAATGAAAGGCTGTTGGTTAGAGAGGTGTAGTAAAGTACATAACATTCTTTCTAATGAAGAGGCAGGTGTCACACTGGGCAGATGTTTAGAGACTCTATGGATTTCAAACCTTCCAATTTTGAGCAGTCTATACAATGGGAGTGTGCAATCAGTGGTCTTTCAGAACCTTAAACAATTGTATCTGGATTGTTGTCCAGGCATCATAAATGTCTTTCCATCCTCCCAGCTGCCAAAAAACCTGGAAAATCTACATGTTCAGTTTTGTGATAAGTTGCAAACTTTGTTTGAATGTGATTCACAATCTAGTTGTGCAATGCAAAGGTTGAGGAAATTGTATCTACTGGAACTACCGGAGTTGTGGAGCATTGGGATAAAATTGCCAGCTGAACTGACTTTTGTGGTCATCGAATGCCCCAAGTTGCTGAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

1264

Amino Acids

143.94

Weight (kDa)

5.84

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 445 - 561 3.3e-07 Leucine-rich repeat region
LRR_8 PF13855 455 - 512 7.2e-06 Leucine rich repeat
LRR_EndoS PF23952 685 - 769 2.1e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_14 PF23598 745 - 889 3e-07 Leucine-rich repeat region
LRR_14 PF23598 902 - 987 1.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3416
Acc36I ACCTGC 2 cut(s) 384, 3416
Acc65I GGTACC 1 cut(s) 2587
AccB1I GGYRCC 3 cut(s) 437, 2495, 2587
AccB7I CCANNNNNTGG 1 cut(s) 572
AccI GTMKAC 3 cut(s) 486, 2131, 2544
AciI CCGC 1 cut(s) 707
AclWI GGATC 7 cut(s) 50, 63, 491, 1116, 1337, 1402, 3253
AcoI YGGCCR 1 cut(s) 1174
AcuI CTGAAG 3 cut(s) 1482, 1631, 3160
AfaI GTAC 7 cut(s) 13, 1197, 2589, 2673, 3155, 3188, 3399
AfiI CCNNNNNNNGG 3 cut(s) 447, 572, 678
AflII CTTAAG 2 cut(s) 1366, 1856
AflIII ACRYGT 1 cut(s) 3613
AhdI GACNNNNNGTC 1 cut(s) 920
AjiI CACGTC 1 cut(s) 1022
AjnI CCWGG 6 cut(s) 456, 565, 1142, 2529, 3556, 3600
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
AloI GAACNNNNNNTCC 6 cut(s) 2697, 2698, 2729, 2730, 3080, 3112
Alw21I GWGCWC 2 cut(s) 1314, 3222
Alw26I GTCTC 4 cut(s) 1217, 1620, 3260, 3446
AlwI GGATC 7 cut(s) 50, 63, 491, 1116, 1337, 1402, 3253
AlwNI CAGNNNCTG 1 cut(s) 1320
AoxI GGCC 3 cut(s) 3, 1174, 1860
ApeKI GCWGC 8 cut(s) 212, 284, 471, 805, 1640, 2286, 2893, 3589
ArsI GACNNNNNNTTYG 2 cut(s) 1619, 1651
Asp700I GAANNNNTTC 3 cut(s) 2166, 2319, 3054
Asp718I GGTACC 1 cut(s) 2587
AspA2I CCTAGG 1 cut(s) 1253
AspS9I GGNCC 3 cut(s) 922, 931, 1861
AsuHPI GGTGA 4 cut(s) 403, 1116, 2402, 3146
AvaII GGWCC 2 cut(s) 922, 931
AvrII CCTAGG 1 cut(s) 1253
AxyI CCTNAGG 2 cut(s) 7, 3261
BaeI ACNNNNGTAYC 4 cut(s) 2571, 2604, 3524, 3557
BalI TGGCCA 1 cut(s) 1176
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 3 cut(s) 437, 2495, 2587
BarI GAAGNNNNNNTAC 2 cut(s) 2664, 2696
BbsI GAAGAC 2 cut(s) 2037, 3365
Bbv12I GWGCWC 2 cut(s) 1314, 3222
BbvCI CCTCAGC 2 cut(s) 216, 520
BbvI GCAGC 8 cut(s) 199, 296, 458, 817, 1652, 2273, 2905, 3576
BciT130I CCWGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
BciVI GTATCC 3 cut(s) 1285, 1843, 2337
BclI TGATCA 1 cut(s) 846
BcoDI GTCTC 4 cut(s) 1217, 1620, 3260, 3446
BfaI CTAG 7 cut(s) 1254, 1485, 2010, 2117, 2801, 3198, 3666
BfmI CTRYAG 4 cut(s) 282, 1815, 2287, 3791
BfrI CTTAAG 2 cut(s) 1366, 1856
BfuAI ACCTGC 2 cut(s) 384, 3416
BfuI GTATCC 3 cut(s) 1285, 1843, 2337
BglII AGATCT 1 cut(s) 278
BisI GCNGC 9 cut(s) 213, 285, 472, 708, 806, 1641, 2287, 2894, 3590
BlnI CCTAGG 1 cut(s) 1253
BlsI GCNGC 9 cut(s) 214, 286, 473, 709, 807, 1642, 2288, 2895, 3591
BmcAI AGTACT 2 cut(s) 2673, 3155
Bme1390I CCNGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
Bme18I GGWCC 2 cut(s) 922, 931
BmeRI GACNNNNNGTC 1 cut(s) 920
BmgBI CACGTC 1 cut(s) 1022
BmgT120I GGNCC 3 cut(s) 922, 931, 1861
BmiI GGNNCC 8 cut(s) 57, 439, 670, 1448, 2195, 2497, 2589, 2842
BmrFI CCNGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
BmrI ACTGGG 2 cut(s) 2980, 3446
BmsI GCATC 6 cut(s) 199, 234, 869, 1191, 2994, 3570
BmuI ACTGGG 2 cut(s) 2980, 3446
BpiI GAAGAC 2 cut(s) 2037, 3365
BplI GAGNNNNNCTC 2 cut(s) 2710, 2742
BpmI CTGGAG 3 cut(s) 2285, 2552, 2840
Bpu10I CCTNAGC 2 cut(s) 216, 520
BpuEI CTTGAG 7 cut(s) 1397, 1565, 2477, 2495, 2705, 2932, 3266
Bsa29I ATCGAT 1 cut(s) 1185
BsaAI YACGTR 2 cut(s) 3010, 3326
BsaJI CCNNGG 5 cut(s) 672, 1143, 1253, 1569, 1784
BsaWI WCCGGW 1 cut(s) 3712
BsaXI ACNNNNNCTCC 2 cut(s) 2234, 2264
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 572, 678
Bse21I CCTNAGG 2 cut(s) 7, 3261
Bse3DI GCAATG 2 cut(s) 761, 3681
BseBI CCWGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
BseCI ATCGAT 1 cut(s) 1185
BseDI CCNNGG 5 cut(s) 672, 1143, 1253, 1569, 1784
BseLI CCNNNNNNNGG 3 cut(s) 447, 572, 678
BseMI GCAATG 2 cut(s) 761, 3681
BseMII CTCAG 9 cut(s) 207, 249, 534, 944, 1327, 2370, 2434, 2629, 2640
BseRI GAGGAG 2 cut(s) 233, 365
BseXI GCAGC 8 cut(s) 199, 296, 458, 817, 1652, 2273, 2905, 3576
BseYI CCCAGC 3 cut(s) 445, 2396, 3585
BshFI GGCC 3 cut(s) 5, 1176, 1862
BshNI GGYRCC 3 cut(s) 437, 2495, 2587
BshVI ATCGAT 1 cut(s) 1185
BsiHKAI GWGCWC 2 cut(s) 1314, 3222
BsiSI CCGG 1 cut(s) 3713
BslI CCNNNNNNNGG 3 cut(s) 447, 572, 678
BsmAI GTCTC 4 cut(s) 1217, 1620, 3260, 3446
BsmI GAATGC 4 cut(s) 1036, 1098, 3299, 3776
BsnI GGCC 3 cut(s) 5, 1176, 1862
Bsp1286I GDGCHC 2 cut(s) 1314, 3222
BspACI CCGC 1 cut(s) 707
BspANI GGCC 3 cut(s) 5, 1176, 1862
BspCNI CTCAG 9 cut(s) 208, 250, 533, 945, 1326, 2369, 2433, 2628, 2639
BspDI ATCGAT 1 cut(s) 1185
BspLI GGNNCC 8 cut(s) 57, 439, 670, 1448, 2195, 2497, 2589, 2842
BspMAI CTGCAG 2 cut(s) 286, 2291
BspMI ACCTGC 2 cut(s) 384, 3416
BspPI GGATC 7 cut(s) 50, 63, 491, 1116, 1337, 1402, 3253
BspQI GCTCTTC 1 cut(s) 1650
BspT107I GGYRCC 3 cut(s) 437, 2495, 2587
BspTI CTTAAG 2 cut(s) 1366, 1856
BsrDI GCAATG 2 cut(s) 761, 3681
BssECI CCNNGG 5 cut(s) 672, 1143, 1253, 1569, 1784
BssNAI GTATAC 1 cut(s) 487
BssT1I CCWWGG 2 cut(s) 1253, 1569
Bst1107I GTATAC 1 cut(s) 487
Bst2UI CCWGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
Bst4CI ACNGT 7 cut(s) 73, 484, 1515, 1836, 2083, 2587, 2622
Bst6I CTCTTC 4 cut(s) 264, 427, 1650, 3414
BstAFI CTTAAG 2 cut(s) 1366, 1856
BstAPI GCANNNNNTGC 1 cut(s) 802
BstBAI YACGTR 2 cut(s) 3010, 3326
BstC8I GCNNGC 3 cut(s) 950, 1098, 3744
BstMAI GTCTC 4 cut(s) 1217, 1620, 3260, 3446
BstMWI GCNNNNNNNGC 8 cut(s) 802, 1215, 1585, 1726, 2200, 2397, 2815, 2902
BstNI CCWGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
BstNSI RCATGY 3 cut(s) 952, 1209, 3617
BstSCI CCNGG 6 cut(s) 456, 565, 1142, 2529, 3556, 3600
BstSFI CTRYAG 4 cut(s) 282, 1815, 2287, 3791
BstSNI TACGTA 2 cut(s) 3010, 3326
BstV1I GCAGC 8 cut(s) 199, 296, 458, 817, 1652, 2273, 2905, 3576
BstV2I GAAGAC 2 cut(s) 2037, 3365
BstX2I RGATCY 3 cut(s) 55, 278, 496
BstYI RGATCY 3 cut(s) 55, 278, 496
BstZ17I GTATAC 1 cut(s) 487
Bsu15I ATCGAT 1 cut(s) 1185
Bsu36I CCTNAGG 2 cut(s) 7, 3261
BsuI GTATCC 3 cut(s) 1285, 1843, 2337
BsuRI GGCC 3 cut(s) 5, 1176, 1862
BsuTUI ATCGAT 1 cut(s) 1185
BtgZI GCGATG 1 cut(s) 441
BtrI CACGTC 1 cut(s) 1022
BtsI GCAGTG 1 cut(s) 3022
BtsIMutI CAGTG 5 cut(s) 507, 1184, 3022, 3434, 3519
BveI ACCTGC 2 cut(s) 384, 3416
Cac8I GCNNGC 3 cut(s) 950, 1098, 3744
CaiI CAGNNNCTG 1 cut(s) 1320
Cfr13I GGNCC 3 cut(s) 922, 931, 1861
ClaI ATCGAT 1 cut(s) 1185
Csp6I GTAC 7 cut(s) 12, 1196, 2588, 2672, 3154, 3187, 3398
CspCI CAANNNNNGTGG 2 cut(s) 805, 840
CviQI GTAC 7 cut(s) 12, 1196, 2588, 2672, 3154, 3187, 3398
DraI TTTAAA 2 cut(s) 1755, 2889
DriI GACNNNNNGTC 1 cut(s) 920
EaeI YGGCCR 1 cut(s) 1174
Eam1104I CTCTTC 4 cut(s) 264, 427, 1650, 3414
Eam1105I GACNNNNNGTC 1 cut(s) 920
EarI CTCTTC 4 cut(s) 264, 427, 1650, 3414
Eco105I TACGTA 2 cut(s) 3010, 3326
Eco130I CCWWGG 2 cut(s) 1253, 1569
Eco32I GATATC 2 cut(s) 1383, 2871
Eco47I GGWCC 2 cut(s) 922, 931
Eco57I CTGAAG 3 cut(s) 1482, 1631, 3160
Eco81I CCTNAGG 2 cut(s) 7, 3261
EcoO109I RGGNCCY 2 cut(s) 922, 1861
EcoRI GAATTC 2 cut(s) 612, 2525
EcoRII CCWGG 6 cut(s) 456, 565, 1142, 2529, 3556, 3600
EcoRV GATATC 2 cut(s) 1383, 2871
EcoT14I CCWWGG 2 cut(s) 1253, 1569
EcoT22I ATGCAT 1 cut(s) 763
ErhI CCWWGG 2 cut(s) 1253, 1569
FalI AAGNNNNNCTT 2 cut(s) 1882, 1914
FauNDI CATATG 1 cut(s) 2538
FbaI TGATCA 1 cut(s) 846
FblI GTMKAC 3 cut(s) 486, 2131, 2544
Fnu4HI GCNGC 9 cut(s) 213, 285, 472, 708, 806, 1641, 2287, 2894, 3590
Fsp4HI GCNGC 9 cut(s) 213, 285, 472, 708, 806, 1641, 2287, 2894, 3590
FspBI CTAG 7 cut(s) 1254, 1485, 2010, 2117, 2801, 3198, 3666
GluI GCNGC 9 cut(s) 213, 285, 472, 708, 806, 1641, 2287, 2894, 3590
GsaI CCCAGC 3 cut(s) 449, 2400, 3589
GsuI CTGGAG 3 cut(s) 2285, 2552, 2840
HaeIII GGCC 3 cut(s) 5, 1176, 1862
HapII CCGG 1 cut(s) 3713
HincII GTYRAC 7 cut(s) 370, 1285, 2132, 2512, 2560, 2968, 3310
HindII GTYRAC 7 cut(s) 370, 1285, 2132, 2512, 2560, 2968, 3310
HindIII AAGCTT 5 cut(s) 263, 1363, 1558, 2165, 2444
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 1 cut(s) 3713
HphI GGTGA 4 cut(s) 403, 1116, 2402, 3146
Hpy99I CGWCG 1 cut(s) 1023
HpyCH4III ACNGT 7 cut(s) 73, 484, 1515, 1836, 2083, 2587, 2622
HpyCH4IV ACGT 5 cut(s) 107, 1021, 2155, 3009, 3325
HpyF10VI GCNNNNNNNGC 8 cut(s) 802, 1215, 1585, 1726, 2200, 2397, 2815, 2902
HpySE526I ACGT 5 cut(s) 107, 1021, 2155, 3009, 3325
KpnI GGTACC 1 cut(s) 2591
Ksp22I TGATCA 1 cut(s) 846
KspAI GTTAAC 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 1650
LmnI GCTCC 6 cut(s) 43, 1446, 1734, 2199, 2533, 3723
Lsp1109I GCAGC 8 cut(s) 199, 296, 458, 817, 1652, 2273, 2905, 3576
LweI GCATC 6 cut(s) 199, 234, 869, 1191, 2994, 3570
MaeI CTAG 7 cut(s) 1254, 1485, 2010, 2117, 2801, 3198, 3666
MaeII ACGT 5 cut(s) 107, 1021, 2155, 3009, 3325
MaeIII GTNAC 5 cut(s) 1201, 1436, 2408, 3017, 3430
MfeI CAATTG 2 cut(s) 1541, 3536
MflI RGATCY 3 cut(s) 55, 278, 496
MhlI GDGCHC 2 cut(s) 1314, 3222
MlsI TGGCCA 1 cut(s) 1176
MluNI TGGCCA 1 cut(s) 1176
MlyI GAGTC 5 cut(s) 524, 1219, 2728, 2813, 3448
MmeI TCCRAC 4 cut(s) 609, 666, 1790, 2136
Mox20I TGGCCA 1 cut(s) 1176
Mph1103I ATGCAT 1 cut(s) 763
MroXI GAANNNNTTC 3 cut(s) 2166, 2319, 3054
MscI TGGCCA 1 cut(s) 1176
MslI CAYNNNNRTG 5 cut(s) 825, 837, 1307, 1710, 3114
Msp20I TGGCCA 1 cut(s) 1176
MspA1I CMGCKG 2 cut(s) 3589, 3746
MspCI CTTAAG 2 cut(s) 1366, 1856
MspI CCGG 1 cut(s) 3713
MspR9I CCNGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
MssI GTTTAAAC 1 cut(s) 1755
MunI CAATTG 2 cut(s) 1541, 3536
Mva1269I GAATGC 4 cut(s) 1036, 1098, 3299, 3776
MvaI CCWGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
MwoI GCNNNNNNNGC 8 cut(s) 802, 1215, 1585, 1726, 2200, 2397, 2815, 2902
NdeI CATATG 1 cut(s) 2538
NlaIV GGNNCC 8 cut(s) 57, 439, 670, 1448, 2195, 2497, 2589, 2842
NmeAIII GCCGAG 2 cut(s) 273, 1765
NmuCI GTSAC 3 cut(s) 2408, 3017, 3430
NsiI ATGCAT 1 cut(s) 763
NspI RCATGY 3 cut(s) 952, 1209, 3617
PaeI GCATGC 1 cut(s) 952
PaqCI CACCTGC 1 cut(s) 3416
PciI ACATGT 1 cut(s) 3613
PciSI GCTCTTC 1 cut(s) 1650
PcsI WCGNNNNNNNCGW 2 cut(s) 751, 1939
PctI GAATGC 4 cut(s) 1036, 1098, 3299, 3776
PdmI GAANNNNTTC 3 cut(s) 2166, 2319, 3054
PflMI CCANNNNNTGG 1 cut(s) 572
PfoI TCCNGGA 1 cut(s) 2529
PkrI GCNGC 9 cut(s) 214, 286, 473, 709, 807, 1642, 2288, 2895, 3591
PleI GAGTC 5 cut(s) 523, 1219, 2727, 2812, 3448
PmeI GTTTAAAC 1 cut(s) 1755
PpsI GAGTC 5 cut(s) 523, 1219, 2727, 2812, 3448
Ppu21I YACGTR 2 cut(s) 3010, 3326
PpuMI RGGWCCY 1 cut(s) 922
PscI ACATGT 1 cut(s) 3613
Psp5II RGGWCCY 1 cut(s) 922
Psp6I CCWGG 6 cut(s) 456, 565, 1142, 2529, 3556, 3600
PspFI CCCAGC 3 cut(s) 445, 2396, 3585
PspGI CCWGG 6 cut(s) 456, 565, 1142, 2529, 3556, 3600
PspN4I GGNNCC 8 cut(s) 57, 439, 670, 1448, 2195, 2497, 2589, 2842
PspPI GGNCC 3 cut(s) 922, 931, 1861
PspPPI RGGWCCY 1 cut(s) 922
PstI CTGCAG 2 cut(s) 286, 2291
PstNI CAGNNNCTG 1 cut(s) 1320
PsuI RGATCY 3 cut(s) 55, 278, 496
PvuII CAGCTG 2 cut(s) 3589, 3746
RsaI GTAC 7 cut(s) 13, 1197, 2589, 2673, 3155, 3188, 3399
RsaNI GTAC 7 cut(s) 12, 1196, 2588, 2672, 3154, 3187, 3398
RseI CAYNNNNRTG 5 cut(s) 825, 837, 1307, 1710, 3114
SalI GTCGAC 1 cut(s) 2130
SapI GCTCTTC 1 cut(s) 1650
SatI GCNGC 9 cut(s) 213, 285, 472, 708, 806, 1641, 2287, 2894, 3590
Sau96I GGNCC 3 cut(s) 922, 931, 1861
ScaI AGTACT 2 cut(s) 2673, 3155
SchI GAGTC 5 cut(s) 524, 1219, 2728, 2813, 3448
ScrFI CCNGG 6 cut(s) 458, 567, 1144, 2531, 3558, 3602
SduI GDGCHC 2 cut(s) 1314, 3222
SfaNI GCATC 6 cut(s) 199, 234, 869, 1191, 2994, 3570
SfcI CTRYAG 4 cut(s) 282, 1815, 2287, 3791
SinI GGWCC 2 cut(s) 922, 931
SmiMI CAYNNNNRTG 5 cut(s) 825, 837, 1307, 1710, 3114
SmlI CTYRAG 9 cut(s) 1366, 1376, 1580, 1856, 2456, 2474, 2684, 2911, 3281
SmoI CTYRAG 9 cut(s) 1366, 1376, 1580, 1856, 2456, 2474, 2684, 2911, 3281
SnaBI TACGTA 2 cut(s) 3010, 3326
SphI GCATGC 1 cut(s) 952
SsiI CCGC 1 cut(s) 707
SspI AATATT 1 cut(s) 694
SspMI CTAG 7 cut(s) 1254, 1485, 2010, 2117, 2801, 3198, 3666
StyD4I CCNGG 6 cut(s) 456, 565, 1142, 2529, 3556, 3600
StyI CCWWGG 2 cut(s) 1253, 1569
TaaI ACNGT 7 cut(s) 73, 484, 1515, 1836, 2083, 2587, 2622
TaiI ACGT 5 cut(s) 110, 1024, 2158, 3012, 3328
TaqI TCGA 5 cut(s) 1018, 1185, 2062, 2131, 3768
TatI WGTACW 4 cut(s) 2671, 3153, 3186, 3397
TauI GCSGC 1 cut(s) 710
TscAI CASTG 5 cut(s) 514, 1184, 3022, 3441, 3519
TseFI GTSAC 3 cut(s) 2408, 3017, 3430
TseI GCWGC 8 cut(s) 212, 284, 471, 805, 1640, 2286, 2893, 3589
Tsp45I GTSAC 3 cut(s) 2408, 3017, 3430
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 5 cut(s) 514, 1184, 3022, 3441, 3519
Van91I CCANNNNNTGG 1 cut(s) 572
Vha464I CTTAAG 2 cut(s) 1366, 1856
VpaK11BI GGWCC 2 cut(s) 922, 931
XbaI TCTAGA 1 cut(s) 2800
XceI RCATGY 3 cut(s) 952, 1209, 3617
XcmI CCANNNNNNNNNTGG 2 cut(s) 592, 1165
XmaJI CCTAGG 1 cut(s) 1253
XmiI GTMKAC 3 cut(s) 486, 2131, 2544
XmnI GAANNNNTTC 3 cut(s) 2166, 2319, 3054
XspI CTAG 7 cut(s) 1254, 1485, 2010, 2117, 2801, 3198, 3666
ZrmI AGTACT 2 cut(s) 2673, 3155
Zsp2I ATGCAT 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.