Rh3AG226600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0009232
Physical Location & Seq
Reverse (-)
23370244 .. 23374248
4005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG226600.1

Sequence Viewer

Length: 4005 bp
ATGGTCTTAGGTACAAAGGAGGAACATAAAAAGAAGATATTGGAGCTGTTGAAGGATCCCAATGTGTCAACAGTAGTTCTTGATGGGAAGCGTGGAGTAGGGAAAACGAGGATAGCAAAAGAGATAATCATGGATGAAGATTTCCATGGCACTCTCTGGCTGTATGTGAATGACAATTATGACACAGAATCTCTTCACAAAAACATTGCTCGCCAGCTATCTCTTTTCTCTATTTATGAGGAATGGGAAGATGATGATGCTGTCGAGGAGGAGCAGAGCTTGGAAAAGTTGAAGCCGAGAATATCTGTGAAGCTTGAAGAATTCAGATCTGCAGCACATGAAAAACATAAACTGTTTCTTCTAGTTCTGGATGATGTTCCTCATCAGAAGGATGTAGAGGAGATTGTGGAAGAAGTTAACAACCTGCTGACAAATGACCGCTCGTCTTTCAAGGTTTTAATCACCAGAGGCACCACACTCGGGATGAACCAGGAAATTAAGAGCTGCAATACAAAAGTACGCCAGATAAATCCATTGTCCACGGATGAGTCCTTGGCTTTATTGCGTGAAAGAACCAAGAAAGAAGTTTTTGATCGTCCAAGCTTTGAAAAACTATCCACAGCTATTGTGGAGATGAGTAAGGGTATTCCTACCATACTTATCACCATAGCAAAAGCAATAAATTACCTGGAAGATGAATGTTCTGGCGTTTGGTCGTTGGAAAATGTTTTGGAAGAAGCAGCTTATGATGGGGAAACTGCTATAAATCTGCTACTTCGCTCTTGGTGTAATGCATTGCCAACCGGTGCTCCGACCGACTGCTTTTGGCATACCCTGCAGCTTCTCAACAAACATGGTGGTGTTCATTACAATGAGTTGATCACACAATGGATAATGGAAGGATATTTTGGATGCAACAACCAGATTGGGAAGGTCTATGAGCAAGGACATAAGGTTCTGATGGACCTTATAGATTGGGGCATGCTGACAAAACAAGAGGATAATATGGTTGTCATGGGAGGAATAATTTTGAGTATAACTGGTCATTGCCGACGTGAATTGAATGAGACTGCAAGGCTTGGATTGGCTAATGTGTTTGAAGATGAAAATTGGAAAGGTCTTGGAATGCTTGCACATACGGATCGTATGATAAAATCACCATCATGTCATAAGACCTGGGAGAAAGTTTCCACACTTCTGATGGATGGGCATTGCATTGATAGGGAAGTACCTGTAACATGCTTTCAACAAATGAAGACTCTCCAAGTTCTCATGTTAATCAAGCCTAGGTTCTCTCTGCTAGTACCCTTGGTTGGCATAAATAACCTTTCCATTCTTGTGCTTAGATTCTGTGATCGCTTGGAGAAGATTGATCCTATCCGAGAATTTCAAAGCTTAAGTGTTCTTGAGATATCTGGTGCTACTTTGTTGGAGATGATCCCCAATGACTTTTTTACAAAGATGCATAAGCTTCGAAGCGTTAACCTTTCTGAAGCCAAGGTCAAATCACTACCTAGTTCTCTTTTTGAAAGAAGTGAACTACGGTGGCTCATCCTTAGAGGATGTTCTCAATTGAAAACACTTCCAAGCTTAAAATCCTTGGGAAAGCTCAAGGTGCTTGATCTTTCTGGTGCTACCTCTTTCAGCAGATTCCAAGACAAAACCTTTGCAGCTCTTCAGAAACTTCATACACTTAATCTTTCTAATTCTCAAATTACTCGCTTACCTTTTCTTCATGGTAATGATGAGCTTAATCAGCTCCTATTAGGTGGCTGTGCAAGTTTAAACAGACTTCCAACCTTGAACACAATACCTCGGCTTCAAATTCTAGATCTTTCTGGTGCTACAGGCTTAAAAGAAATACAGTTCGAACCTTTAGATAGCCTTAAGGCCCTTGATTTATCTGGAACTCGTATTGAAAGGCAACCTTCCAGTTTCAGCCACCTTTCTGATCTCTCCTTACGAGAGTGTCATGAGCTTGCAAGACTTCCACTCACAAAAGAAATCAAATATCTTGAATCACTTGACCTTTCTGGTTCTAGCACTAAGGTTGAAATTGAAGACAAATTCTTCCAGCATATGAGGTTTCTTCGAGTTCTCAACTTTTCAACAGTTAAGATAAAAGAACTACCTTCCCTGTCCAACCTAGCTAACCTCTGTCAACTTTTCCTAAAGGATTGTTTATGTTTAGAAAAGCTTCCAGAGATGGAAGGACTTAAAAGGCTCCAAGAGCTTAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCAAACCTGAACTCCCTTGAAAAACTGGAGATTCTTGATATTTCACGTTGCAGGGTTTTGAACCAAATACAAGAGGAATCCTTTCAAAAGATGTCCCATATTCGGACACTCAACCTCTCAGACACTATGATTGAGTTCCTACCATCCATTTGCAAACCCAGCAACCTCTGTCACCTCATCCTCAGAAATTGCAAAAAGTTAAAAAAGCTTCCACCTCTTGATCATTTGTCAAAACTTGAGGAATTGAATCTGTGTGGTGCCAGTTCTTTAGTTGACTTTACATCTGAATTCCTGGAGCCTATGGTCGACCTTCAAATTGTTGACTTATCTGAAATCCCACTGACAAAGGTACCATCCATGTCCAAATTCATCAAACTCAGACAGTTCTCAGTAAAGGGTTGTTCATCCTTAAATGAAGTGATACATCTTGAAGAACTAACACTTCTTGAGATTCTTGATCTTTCTGGAACAGCAGTTCCGAGTCTGCCTCCCCTAAATACTTTTAGCAACCTCCGCCAGCTCTTGCTAAAAGATTGTTTACAGATAGAAGAGTTGCAGTTTCTAGGGTCGCTTAGTCGGCTGGAGGTTCTTGATCTGTCAGGAACCAGAATGAAGCAATTTCCTTATGAGATATCAGAGCTTACTCATTTAGAGCAGCTTCATCTGCCTGACTTGAGGGGTATGCGTGATCTTGACTTGGGAAAGATAAAGCGTTTGCCACAAAAGGTCAACTGGGATGAATGTCACATCTTTGAGTGTGCTGATATTTACATGGGCAGTGACAAGCCTTCCCTATCTGTAAGTGGCTTTGAAATTTTTCACATTTTGGACAAACATCCAAAGTTCTGGGAAGAAAAGTTCAAAAAATTTCATTTCCATGTAAAGAAGAAGGAAGGCGAAGATGGAGGCATCAATCACTGCAAAGATGAATTTGTCTTCAGAGATGTGTACTTCCACACTAGACATTTTTATCTTAAAGAGCACGACAGGTCTTTGGAAATCTATGGATCGTATAGTCTCCCTAAGGGTTTTGAAAGTGTCCTCAAGTGTGCCGAATGCATATCTTTGGTTGACAATGACCTTATACATAGCCTATCTTATTTAGGTTCTGATAATGTGAGGGCAATGAAACGCTGTTGGTTAGAGAGATGTAGTAAAGTACGAAACATTCTTTCTAATGATGAGGCAGGTGTCACACTGGGCAGAAATTTAGAGACTCTATGGATTTCAAACCTCCCAATTTTGAGCAGTCTATATAATGGGAATGTGCAATCAGTGGTGTTTCAGAACCTTAAACAATTGTATCTGGATTGTTGTCCAGGCATTGTAAATGTCTTTCCATCCTCCCAGCTTCCAGAAAACCTGGAAACTCTACATGTTCAGTTTTGTGATAAGTTGCAAACTTTGTTCGAATGTGATTCACAATCTAGTTGTGCAATGCAAAGGTTGAGGAAATTGTATCTACTGGAACTACCAGAGTTGAGTAGCATTGGGATAAAATTGCCAGAAGACCTGGAAATTCTACATGTTCAGTCTTGTGATAAGTTGCAAACTTTGTTTGATTGTGATTCACAATCTAGTTGTGCAATGCAAAGATTGAGGAAATTGTATCTACAGGAACTACCAGAGTTGAGGAGCATTGGGATAAAATTGCCAGCTGAACTGACTTCTGTGGTCATCAAATGCCCCAAGTTGCTGGAGCTCCAGTTGGATGCGACCGACCAGGTCTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

1334

Amino Acids

152.16

Weight (kDa)

5.91

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 7 - 191 4.1e-12 NB-ARC domain
LRR_14 PF23598 454 - 571 9.9e-07 Leucine-rich repeat region
LRR_14 PF23598 693 - 839 8.3e-07 Leucine-rich repeat region
LRR_8 PF13855 764 - 820 5.7e-06 Leucine rich repeat
LRR_14 PF23598 912 - 997 6e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 1160 - 1253 3.5e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3446
Acc36I ACCTGC 2 cut(s) 432, 3446
Acc65I GGTACC 1 cut(s) 2617
AccB1I GGYRCC 3 cut(s) 470, 2525, 2617
AccBSI CCGCTC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 2574
AciI CCGC 2 cut(s) 439, 2782
AclWI GGATC 6 cut(s) 50, 63, 1149, 1367, 1432, 3283
AcuI CTGAAG 3 cut(s) 1512, 1661, 3190
AfaI GTAC 7 cut(s) 13, 519, 1230, 1305, 2619, 3218, 3429
AfiI CCNNNNNNNGG 3 cut(s) 480, 1313, 2370
AflII CTTAAG 2 cut(s) 1396, 1886
AflIII ACRYGT 2 cut(s) 3643, 3793
AgeI ACCGGT 1 cut(s) 803
AhdI GACNNNNNGTC 1 cut(s) 442
AjiI CACGTC 1 cut(s) 1055
AjnI CCWGG 8 cut(s) 489, 687, 1175, 2559, 3586, 3630, 3780, 3990
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AloI GAACNNNNNNTCC 8 cut(s) 939, 971, 2727, 2728, 2759, 2760, 3110, 3142
Alw21I GWGCWC 3 cut(s) 811, 3252, 3972
Alw26I GTCTC 4 cut(s) 1061, 3290, 3476, 4000
AlwI GGATC 6 cut(s) 50, 63, 1149, 1367, 1432, 3283
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 1 cut(s) 1890
ApeKI GCWGC 6 cut(s) 332, 504, 740, 838, 1670, 2923
ArsI GACNNNNNNTTYG 2 cut(s) 1649, 1681
AsiGI ACCGGT 1 cut(s) 803
Asp700I GAANNNNTTC 4 cut(s) 192, 2196, 2349, 3084
Asp718I GGTACC 1 cut(s) 2617
AspA2I CCTAGG 1 cut(s) 1286
AspS9I GGNCC 2 cut(s) 964, 1891
AsuHPI GGTGA 4 cut(s) 454, 655, 1149, 2432
AsuII TTCGAA 3 cut(s) 1474, 1869, 3678
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 964
AvrII CCTAGG 1 cut(s) 1286
AxyI CCTNAGG 1 cut(s) 3291
BaeI ACNNNNGTAYC 4 cut(s) 2601, 2634, 3554, 3587
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 3 cut(s) 470, 2525, 2617
BanII GRGCYC 1 cut(s) 3972
BbsI GAAGAC 4 cut(s) 1262, 2067, 3196, 3783
Bbv12I GWGCWC 3 cut(s) 811, 3252, 3972
BbvI GCAGC 6 cut(s) 344, 491, 752, 850, 1682, 2935
BcgI CGANNNNNNTGC 4 cut(s) 460, 494, 1454, 1488
BciT130I CCWGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
BclI TGATCA 2 cut(s) 879, 2488
BcoDI GTCTC 4 cut(s) 1061, 3290, 3476, 4000
BfmI CTRYAG 4 cut(s) 330, 836, 1845, 3881
BfrI CTTAAG 2 cut(s) 1396, 1886
BfuAI ACCTGC 2 cut(s) 432, 3446
BglII AGATCT 2 cut(s) 326, 1831
BisI GCNGC 6 cut(s) 333, 505, 741, 839, 1671, 2924
BlnI CCTAGG 1 cut(s) 1286
BlsI GCNGC 6 cut(s) 334, 506, 742, 840, 1672, 2925
Bme1390I CCNGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
Bme18I GGWCC 1 cut(s) 964
BmeRI GACNNNNNGTC 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 1055
BmgT120I GGNCC 2 cut(s) 964, 1891
BmiI GGNNCC 7 cut(s) 57, 472, 2225, 2527, 2565, 2619, 2872
BmrFI CCNGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
BmrI ACTGGG 2 cut(s) 3010, 3476
BmsI GCATC 5 cut(s) 247, 902, 1452, 3186, 3970
BmuI ACTGGG 2 cut(s) 3010, 3476
BpiI GAAGAC 4 cut(s) 1262, 2067, 3196, 3783
BplI GAGNNNNNCTC 2 cut(s) 2740, 2772
BpmI CTGGAG 5 cut(s) 2315, 2582, 2870, 3956, 3986
Bpu14I TTCGAA 3 cut(s) 1474, 1869, 3678
BpuEI CTTGAG 6 cut(s) 1427, 1595, 2525, 2735, 2962, 3296
BsaI GGTCTC 1 cut(s) 4000
BsaJI CCNNGG 9 cut(s) 145, 540, 552, 1176, 1286, 1308, 1497, 1599, 1814
BsaWI WCCGGW 1 cut(s) 803
BsaXI ACNNNNNCTCC 7 cut(s) 793, 823, 2264, 2294, 3959, 3980, 3989
Bsc4I CCNNNNNNNGG 3 cut(s) 480, 1313, 2370
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 8 cut(s) 1045, 1932, 2298, 2529, 3005, 3471, 3738, 3973
Bse21I CCTNAGG 1 cut(s) 3291
Bse3DI GCAATG 7 cut(s) 204, 794, 1045, 1210, 3399, 3711, 3861
BseBI CCWGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
BseDI CCNNGG 9 cut(s) 145, 540, 552, 1176, 1286, 1308, 1497, 1599, 1814
BseLI CCNNNNNNNGG 3 cut(s) 480, 1313, 2370
BseMI GCAATG 7 cut(s) 204, 794, 1045, 1210, 3399, 3711, 3861
BseMII CTCAG 4 cut(s) 2400, 2464, 2659, 2670
BseNI ACTGG 8 cut(s) 1045, 1932, 2298, 2529, 3005, 3471, 3738, 3973
BseRI GAGGAG 4 cut(s) 281, 284, 413, 3916
BseXI GCAGC 6 cut(s) 344, 491, 752, 850, 1682, 2935
BseYI CCCAGC 2 cut(s) 2426, 3615
Bsh1285I CGRYCG 2 cut(s) 816, 3987
BshFI GGCC 1 cut(s) 1892
BshNI GGYRCC 3 cut(s) 470, 2525, 2617
BshTI ACCGGT 1 cut(s) 803
BsiEI CGRYCG 2 cut(s) 816, 3987
BsiHKAI GWGCWC 3 cut(s) 811, 3252, 3972
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 1 cut(s) 804
BslFI GGGAC 1 cut(s) 2347
BslI CCNNNNNNNGG 3 cut(s) 480, 1313, 2370
BsmAI GTCTC 4 cut(s) 1061, 3290, 3476, 4000
BsmFI GGGAC 1 cut(s) 2347
BsmI GAATGC 2 cut(s) 1131, 3329
BsnI GGCC 1 cut(s) 1892
Bso31I GGTCTC 1 cut(s) 4000
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 3 cut(s) 1474, 1869, 3678
Bsp1286I GDGCHC 3 cut(s) 811, 3252, 3972
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 2 cut(s) 439, 2782
BspANI GGCC 1 cut(s) 1892
BspCNI CTCAG 4 cut(s) 2399, 2463, 2658, 2669
BspHI TCATGA 1 cut(s) 1972
BspLI GGNNCC 7 cut(s) 57, 472, 2225, 2527, 2565, 2619, 2872
BspMAI CTGCAG 2 cut(s) 334, 840
BspMI ACCTGC 2 cut(s) 432, 3446
BspPI GGATC 6 cut(s) 50, 63, 1149, 1367, 1432, 3283
BspQI GCTCTTC 1 cut(s) 1680
BspT104I TTCGAA 3 cut(s) 1474, 1869, 3678
BspT107I GGYRCC 3 cut(s) 470, 2525, 2617
BspTI CTTAAG 2 cut(s) 1396, 1886
BspTNI GGTCTC 1 cut(s) 4000
BsrBI CCGCTC 1 cut(s) 441
BsrDI GCAATG 7 cut(s) 204, 794, 1045, 1210, 3399, 3711, 3861
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 8 cut(s) 1045, 1932, 2298, 2529, 3005, 3471, 3738, 3973
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 9 cut(s) 145, 540, 552, 1176, 1286, 1308, 1497, 1599, 1814
BssT1I CCWWGG 6 cut(s) 145, 552, 1286, 1308, 1497, 1599
Bst2UI CCWGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
Bst4CI ACNGT 6 cut(s) 73, 354, 1545, 1866, 2113, 2652
Bst6I CTCTTC 3 cut(s) 198, 1680, 2811
BstAFI CTTAAG 2 cut(s) 1396, 1886
BstAPI GCANNNNNTGC 1 cut(s) 835
BstBI TTCGAA 3 cut(s) 1474, 1869, 3678
BstC8I GCNNGC 7 cut(s) 211, 215, 983, 1131, 1980, 2786, 3924
BstDSI CCRYGG 2 cut(s) 145, 540
BstMAI GTCTC 4 cut(s) 1061, 3290, 3476, 4000
BstMCI CGRYCG 2 cut(s) 816, 3987
BstMWI GCNNNNNNNGC 8 cut(s) 835, 1615, 1756, 2230, 2427, 2781, 2845, 2932
BstNI CCWGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
BstNSI RCATGY 4 cut(s) 985, 1242, 3647, 3797
BstSCI CCNGG 8 cut(s) 489, 687, 1175, 2559, 3586, 3630, 3780, 3990
BstSFI CTRYAG 4 cut(s) 330, 836, 1845, 3881
BstV1I GCAGC 6 cut(s) 344, 491, 752, 850, 1682, 2935
BstV2I GAAGAC 4 cut(s) 1262, 2067, 3196, 3783
BstX2I RGATCY 3 cut(s) 55, 326, 1831
BstXI CCANNNNNNTGG 2 cut(s) 3114, 3964
BstYI RGATCY 3 cut(s) 55, 326, 1831
Bsu36I CCTNAGG 1 cut(s) 3291
BsuRI GGCC 1 cut(s) 1892
BtgI CCRYGG 2 cut(s) 145, 540
BtrI CACGTC 1 cut(s) 1055
BtsI GCAGTG 2 cut(s) 3052, 3184
BtsIMutI CAGTG 5 cut(s) 2606, 3052, 3184, 3464, 3549
BveI ACCTGC 2 cut(s) 432, 3446
Cac8I GCNNGC 7 cut(s) 211, 215, 983, 1131, 1980, 2786, 3924
CciI TCATGA 1 cut(s) 1972
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 2 cut(s) 964, 1891
CsiI ACCWGGT 1 cut(s) 3990
Csp6I GTAC 7 cut(s) 12, 518, 1229, 1304, 2618, 3217, 3428
CspAI ACCGGT 1 cut(s) 803
CspCI CAANNNNNGTGG 4 cut(s) 607, 642, 838, 873
CviQI GTAC 7 cut(s) 12, 518, 1229, 1304, 2618, 3217, 3428
DraI TTTAAA 1 cut(s) 1785
DriI GACNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 198, 1680, 2811
Eam1105I GACNNNNNGTC 1 cut(s) 442
EarI CTCTTC 3 cut(s) 198, 1680, 2811
EciI GGCGGA 1 cut(s) 2771
Ecl136II GAGCTC 1 cut(s) 3970
Eco130I CCWWGG 6 cut(s) 145, 552, 1286, 1308, 1497, 1599
Eco24I GRGCYC 1 cut(s) 3972
Eco31I GGTCTC 1 cut(s) 4000
Eco32I GATATC 2 cut(s) 1413, 2901
Eco47I GGWCC 1 cut(s) 964
Eco53kI GAGCTC 1 cut(s) 3970
Eco57I CTGAAG 3 cut(s) 1512, 1661, 3190
Eco81I CCTNAGG 1 cut(s) 3291
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 3970
EcoO109I RGGNCCY 1 cut(s) 1891
EcoRI GAATTC 2 cut(s) 320, 2555
EcoRII CCWGG 8 cut(s) 489, 687, 1175, 2559, 3586, 3630, 3780, 3990
EcoRV GATATC 2 cut(s) 1413, 2901
EcoT14I CCWWGG 6 cut(s) 145, 552, 1286, 1308, 1497, 1599
EcoT22I ATGCAT 3 cut(s) 796, 1467, 3329
EcoT38I GRGCYC 1 cut(s) 3972
ErhI CCWWGG 6 cut(s) 145, 552, 1286, 1308, 1497, 1599
FalI AAGNNNNNCTT 2 cut(s) 1912, 1944
FaqI GGGAC 1 cut(s) 2347
FauNDI CATATG 1 cut(s) 2079
FbaI TGATCA 2 cut(s) 879, 2488
FblI GTMKAC 1 cut(s) 2574
Fnu4HI GCNGC 6 cut(s) 333, 505, 741, 839, 1671, 2924
FriOI GRGCYC 1 cut(s) 3972
Fsp4HI GCNGC 6 cut(s) 333, 505, 741, 839, 1671, 2924
GluI GCNGC 6 cut(s) 333, 505, 741, 839, 1671, 2924
GsaI CCCAGC 2 cut(s) 2430, 3619
GsuI CTGGAG 5 cut(s) 2315, 2582, 2870, 3956, 3986
HaeIII GGCC 1 cut(s) 1892
HapII CCGG 1 cut(s) 804
HincII GTYRAC 9 cut(s) 69, 418, 1483, 2162, 2542, 2575, 2590, 2998, 3340
HindII GTYRAC 9 cut(s) 69, 418, 1483, 2162, 2542, 2575, 2590, 2998, 3340
HindIII AAGCTT 7 cut(s) 311, 601, 1393, 1469, 1588, 2195, 2474
HpaI GTTAAC 2 cut(s) 418, 1483
HpaII CCGG 1 cut(s) 804
HphI GGTGA 4 cut(s) 454, 655, 1149, 2432
Hpy99I CGWCG 1 cut(s) 1056
HpyCH4III ACNGT 6 cut(s) 73, 354, 1545, 1866, 2113, 2652
HpyCH4IV ACGT 2 cut(s) 1054, 2314
HpyF10VI GCNNNNNNNGC 8 cut(s) 835, 1615, 1756, 2230, 2427, 2781, 2845, 2932
HpySE526I ACGT 2 cut(s) 1054, 2314
KpnI GGTACC 1 cut(s) 2621
Ksp22I TGATCA 2 cut(s) 879, 2488
KspAI GTTAAC 2 cut(s) 418, 1483
LguI GCTCTTC 1 cut(s) 1680
LmnI GCTCC 9 cut(s) 43, 271, 814, 1764, 2229, 2563, 3903, 3967, 3975
Lsp1109I GCAGC 6 cut(s) 344, 491, 752, 850, 1682, 2935
LweI GCATC 5 cut(s) 247, 902, 1452, 3186, 3970
MabI ACCWGGT 1 cut(s) 3990
MaeII ACGT 2 cut(s) 1054, 2314
MaeIII GTNAC 5 cut(s) 1234, 2438, 3011, 3047, 3460
MbiI CCGCTC 1 cut(s) 441
MfeI CAATTG 2 cut(s) 1571, 3566
MflI RGATCY 3 cut(s) 55, 326, 1831
MhlI GDGCHC 3 cut(s) 811, 3252, 3972
MlyI GAGTC 4 cut(s) 557, 1252, 2758, 3478
MmeI TCCRAC 6 cut(s) 699, 836, 1410, 1820, 2166, 3957
Mph1103I ATGCAT 3 cut(s) 796, 1467, 3329
MroXI GAANNNNTTC 4 cut(s) 192, 2196, 2349, 3084
MslI CAYNNNNRTG 6 cut(s) 858, 870, 1162, 1337, 1740, 3144
MspA1I CMGCKG 1 cut(s) 3926
MspCI CTTAAG 2 cut(s) 1396, 1886
MspI CCGG 1 cut(s) 804
MspR9I CCNGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
MssI GTTTAAAC 1 cut(s) 1785
MunI CAATTG 2 cut(s) 1571, 3566
Mva1269I GAATGC 2 cut(s) 1131, 3329
MvaI CCWGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
MwoI GCNNNNNNNGC 8 cut(s) 835, 1615, 1756, 2230, 2427, 2781, 2845, 2932
NcoI CCATGG 1 cut(s) 145
NdeI CATATG 1 cut(s) 2079
NlaIV GGNNCC 7 cut(s) 57, 472, 2225, 2527, 2565, 2619, 2872
NmeAIII GCCGAG 2 cut(s) 321, 1795
NmuCI GTSAC 4 cut(s) 2438, 3011, 3047, 3460
NsiI ATGCAT 3 cut(s) 796, 1467, 3329
NspI RCATGY 4 cut(s) 985, 1242, 3647, 3797
NspV TTCGAA 3 cut(s) 1474, 1869, 3678
PaeI GCATGC 1 cut(s) 985
PagI TCATGA 1 cut(s) 1972
PaqCI CACCTGC 1 cut(s) 3446
PciI ACATGT 2 cut(s) 3643, 3793
PciSI GCTCTTC 1 cut(s) 1680
PctI GAATGC 2 cut(s) 1131, 3329
PdmI GAANNNNTTC 4 cut(s) 192, 2196, 2349, 3084
PflFI GACNNNGTC 1 cut(s) 3992
PfoI TCCNGGA 1 cut(s) 2559
PinAI ACCGGT 1 cut(s) 803
PkrI GCNGC 6 cut(s) 334, 506, 742, 840, 1672, 2925
PleI GAGTC 4 cut(s) 556, 1252, 2757, 3478
PmeI GTTTAAAC 1 cut(s) 1785
PpsI GAGTC 4 cut(s) 556, 1252, 2757, 3478
PscI ACATGT 2 cut(s) 3643, 3793
Psp124BI GAGCTC 1 cut(s) 3972
Psp6I CCWGG 8 cut(s) 489, 687, 1175, 2559, 3586, 3630, 3780, 3990
PspFI CCCAGC 2 cut(s) 2426, 3615
PspGI CCWGG 8 cut(s) 489, 687, 1175, 2559, 3586, 3630, 3780, 3990
PspN4I GGNNCC 7 cut(s) 57, 472, 2225, 2527, 2565, 2619, 2872
PspPI GGNCC 2 cut(s) 964, 1891
PstI CTGCAG 2 cut(s) 334, 840
PsuI RGATCY 3 cut(s) 55, 326, 1831
PsyI GACNNNGTC 1 cut(s) 3992
PvuII CAGCTG 1 cut(s) 3926
RsaI GTAC 7 cut(s) 13, 519, 1230, 1305, 2619, 3218, 3429
RsaNI GTAC 7 cut(s) 12, 518, 1229, 1304, 2618, 3217, 3428
RseI CAYNNNNRTG 6 cut(s) 858, 870, 1162, 1337, 1740, 3144
SacI GAGCTC 1 cut(s) 3972
SalI GTCGAC 1 cut(s) 2573
SapI GCTCTTC 1 cut(s) 1680
SatI GCNGC 6 cut(s) 333, 505, 741, 839, 1671, 2924
Sau96I GGNCC 2 cut(s) 964, 1891
SchI GAGTC 4 cut(s) 557, 1252, 2758, 3478
ScrFI CCNGG 8 cut(s) 491, 689, 1177, 2561, 3588, 3632, 3782, 3992
SduI GDGCHC 3 cut(s) 811, 3252, 3972
SexAI ACCWGGT 1 cut(s) 3990
SfaNI GCATC 5 cut(s) 247, 902, 1452, 3186, 3970
SfcI CTRYAG 4 cut(s) 330, 836, 1845, 3881
SfuI TTCGAA 3 cut(s) 1474, 1869, 3678
SinI GGWCC 1 cut(s) 964
SmiMI CAYNNNNRTG 6 cut(s) 858, 870, 1162, 1337, 1740, 3144
SmlI CTYRAG 8 cut(s) 1396, 1406, 1610, 1886, 2504, 2714, 2941, 3311
SmoI CTYRAG 8 cut(s) 1396, 1406, 1610, 1886, 2504, 2714, 2941, 3311
SphI GCATGC 1 cut(s) 985
SsiI CCGC 2 cut(s) 439, 2782
SstI GAGCTC 1 cut(s) 3972
StyD4I CCNGG 8 cut(s) 489, 687, 1175, 2559, 3586, 3630, 3780, 3990
StyI CCWWGG 6 cut(s) 145, 552, 1286, 1308, 1497, 1599
TaaI ACNGT 6 cut(s) 73, 354, 1545, 1866, 2113, 2652
TaiI ACGT 2 cut(s) 1057, 2317
TaqI TCGA 6 cut(s) 264, 1474, 1869, 2092, 2574, 3678
TaqII GACCGA 2 cut(s) 830, 4001
TatI WGTACW 1 cut(s) 3216
TscAI CASTG 5 cut(s) 2613, 3052, 3191, 3471, 3549
TseFI GTSAC 4 cut(s) 2438, 3011, 3047, 3460
TseI GCWGC 6 cut(s) 332, 504, 740, 838, 1670, 2923
Tsp45I GTSAC 4 cut(s) 2438, 3011, 3047, 3460
TspGWI ACGGA 2 cut(s) 557, 1154
TspRI CASTG 5 cut(s) 2613, 3052, 3191, 3471, 3549
Tth111I GACNNNGTC 1 cut(s) 3992
Vha464I CTTAAG 2 cut(s) 1396, 1886
VpaK11BI GGWCC 1 cut(s) 964
XbaI TCTAGA 1 cut(s) 1828
XceI RCATGY 4 cut(s) 985, 1242, 3647, 3797
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 1198
XmaJI CCTAGG 1 cut(s) 1286
XmiI GTMKAC 1 cut(s) 2574
XmnI GAANNNNTTC 4 cut(s) 192, 2196, 2349, 3084
Zsp2I ATGCAT 3 cut(s) 796, 1467, 3329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.