pycom14g04560

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009232
Physical Location & Seq
Reverse (-)
4182202 .. 4186104
3903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04560.1

Sequence Viewer

Length: 3903 bp
ATGGCTTTAGGTTTGAAGGAACGGATAGAAAAGATACTGGAGACGCTGAAGAATGATGGTGTAACAACAATTGTTCTTGATGGTATGCGTGGAGAATGGAAAACGTGGACGGCAAGAGAGATAAGCATGAAGAGAGACTTAATCCATGACAGTCTATGGGTGTATCTGAATAAACAATATGACAGCAGCTCTCTACTTGAGAACATTGCCGGCCAGCTATCTCTAATCTCTATTCATGAGGAATATGATGGTGACGAGGAGGAGGAGAACATGAGGCTGGAATTGAAACAAAGAATAGCCATCAAGCTTAAAGGATTGATATCCGCAGCACATAAAGAACACAAATATTTTCTACTAATTCTGGATGATGTTCCTTACGAGCAGTCTGTGAAAGACATTATCTCAGAACTGAAGAAGCTGCTGGGTTTGAATGATACTTCTTTTAAGGTTTTAATAACTAGAGATGATGGTGACAAAAAGCACACCACAGAGGAGGAGCGCACCGCAGAGGAGGAGGAGGAGGAGGAGCACACTGCAGAGGAGCATGGAGCAATTCAGACCATCCACACAAGGCTGCACAGCATAAACGTAGATCAATTGTCTACACAAGAGTCCAAGCTTTTATTGAAAGAAAATATCAAGAAAGTAATTTCCGACTCTCCGGGCTTTGAAATGTTATCCAAGGTGATTGTGGAGATGAGAAAGTCCATGCCTAGTGAAATCATCGCAATAGCAAAAGCATTCAATTATGTTGAACATATTGGTTCTGGGGTTTGGTCATTGGAAAGTGTTTCGGAAGCAGCAGCTGATGATGAGGGAGCTGCAATAAATCTGCTACATCGTGCTTGGTATGACACATTGCCAAGCGGTGTTCGGGCAGACTGTTTTTGGCATAGCAAGCAATTTTTCAGCAATAATGTCTGTCTGCATTACAAAGAGTTGATCACTCATTGGATAATGGAAGGGTATGTTGGATGCATTGATCATATCGAGAAAGCCTATGAGCAAGGACATCGCGTTCTGATGGAGCTTATAGATCGTGGCATGCTAAGACGCCAGGAGGATAATATCATGGAAGGAATCTTTTCAAATATAGCTGATCACCGCTGTCGTGGGTTTACTGGGACTGCAAGCATGGGATTGGTTAATGTGTTTGCAAATTCCAAGTGGGAAGGTCTTGGGAGGCTTACACACACTGATGGTATGATAAAATCGCCGTGCAGTGCTAAAACCTGGGGAAAAGTCTCTACACTTGTGATGGATGGACGCTGCCTCAGTAGAGAAGTCCCTGAAAAATATTTTCAACCAATGCAGGAGCTCCGGATATTAACTATATTGAATCCCAGGTTCAGATCTCTACCCTTGTCCTTGACTAGCTTAAAGAACCTTTCTATTCTTGTGCTCAGATGCTGTGATCTCTTAGAGAAAATTGCTCGTCTCCGAGAACTTAAAAACTTAAATGTTCTTGAGATATCTGGTGCAACCTGCTTGGAGAAAATTGATGACGATCTTTTCACAGACATGCCTCACCTTCGAAGCCTTAACCTTTCTGGTGCCAATATCAAATCACTACCTGGTTCTCTCTTTCAAAAAACTGAACTACGGTGGCTCATCCTTAGAGGTTGTTCCCAATTGATAGAGCTTCCAAGCTTGAAATCATTTGAAAACCTCGAGGTGCTTGATCTTCATGGTGCTACATCTTTCAAAAAGTTTCAAGACAAAACCTTTGCTCCGCTTAAGAAACTTCAGTTAATTGATCTTTCCAATTCCCAAATTAGTCGCCTACCTTTTCTTCACAATCTTGGTGATCTCACTCGGCTTCTATTAGCTGGTTGCGGAAGCTTAAAAAGACTGCCCATCTTGAACACACTAACTCAGCTTCAAATTCTTGATCTTTCGGATGCTACAAGTTTACAAGAAATACAAAAAGAACCATTGGATGGCCTTAAAGTCCTTGATTTATCTCAGACTCAAATTAGTGGTCTGCCTTCAAGTGTAAGAAACCTTTCTGATCTTGTCTTAAGAGATTGTTCTCAGCTTGTAAAACTGCCCCCCATAGGAGCAACTAAAGAGCTTGAACAGCTTGACCTTTCCGGCTCAAGAAATCTGGCTGAAATTGAAGACAAATCCTTACAACATATGAGGTTCCTTCGAGTTCTCAACTTTTCGAAAGCCAAAGTAAAAGAATTACCATCTTTGTCCAACCTAGTTAACCTTCGCCAACTCCTGTTAATGGACTGTACGGTTCTAGAGAAACTGCCTGCGATGGAGGGCCTTCGCAGACTCCAGGCTCTCAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCCCACCTGAATGCCCTTGAAAAATTAGAGATTCTTGACGTTTCAGGTTGTACGGCATTGAAAGGAATACATGAAAAGTCCTTTGAAAATATGTCATGCATTCGTACAATCAACCTCTCAGACTCTAAAATTGAGTTTCTACCTTCCCTTCCCAAAGCCAGAATCTTCTGTCATCTCATACTCAGAAATTGCAGCAACCTAAAAAAGCTCCCTCCTCTTGAACATCTGTCAAAACTTGAGGAACTTGATCTATGTGGTGCCATTTCTTTAGTTGACTTTGAAGCTGGGTTCTTGGAGCATATGCATGACCTTAGAATTCTCAACCTATGTGGAATCCCACTTACAATGCTACCGTCCATGTCCAGACTCACCAACCTCAGACAACTCGCACTAAAAGGTTGTTCGTCGCTGGAAACGGTGCAAAACCTTGAAGCACTAACACTGCTTGAGATTCTTGATCTTTCTGGAACAGCAATTGGGAGTCTGCCACCTCTAAACACTTTTAGCAACCTCGTCCAGCTCTTGCTGAAAGATTGTTCACGGATACAAGAGTTGCAGCTGCTAGGATCACTTAGTCGTCTGGAGGTTCTGGATCTTTCAGGAAACAGAATGAAAAAATTTCCCTATGAGATATCTGAATTGACTAGTTTACAGTGCCTTTATCTTCCAGACGTGAGGAGTATGCATGATCTCGATGTTGGAAAGATAAGGCGTATACCACAAGAGGTCAACTGGGATGAATGTGGTATCTTTGAGCATGCTGATATCTTTTGTGACAATGACAAACCTTCCATATCTGTAAGTGGAACGGAAATTTTTCAATTTTTGGACAAAAATCCCAAGGTGTGGGAAACAAAGTTCAAAAGATTTTGTTTCTTTGTAACCAAGAAGCAAGGTCAAGATGGAGATATCATCTGCTGCAAAGATGAAATTGTCAAAGATGTGTACTCAAGCACCAGACACTTGTGTTTTCCTGAAGAGCATGATCGGTCTTTGGAAATCCATGGATCATATAGTCTTCCTCATGGTTTCGAAAGTGTCCTCAAGCATTCCAACTGTATATCTTTGGTTGACAATGACATTATAAGTTGCCTATGTGATTTAGGTGCTGACGTTGTGGACGTAATGAAGGGCTGTTGGATAGAGAGATGCAGTAAAATTGAGAGCATTCTCTGTGGAGCCGAGGAAAATGTCAGACTGGGGAGAAGTTTAGAGATTCTATGGTTTTCGAACCTTCCGAAGTTGAGAAGTCTATACAATGGGAATGCAGAACCAAAGTGCTTTAGGAATCTTAAACATTTGTTTCTAGATTGTTGTCCAATGATTGTGAATGTCTTTCCCTCATCCCAGCTTCCAGAAAACCTCGAAATTCTTCATGTGCAATTTTGTGATAGATTGCAAACTTTGGTTGAATGTGATACAGCATCTAAGTATACATTAAAAAGGTTAAGGACAGTGTATCTGTTGGAACTGCCAAAGTTGACGAGCATTGGGATTGAATTGCCAGATCAAGTGGATGTCGAAGCTATGGAATGCCCTAAGTTTACGAAGGACGCTCCTGCTATGGTTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

1301

Amino Acids

147.12

Weight (kDa)

5.69

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 477 - 607 6.4e-06 Leucine-rich repeat region
LRR_8 PF13855 485 - 542 3.2e-08 Leucine rich repeat
LRR_14 PF23598 709 - 895 2.5e-06 Leucine-rich repeat region
LRR_EndoS PF23952 715 - 798 2e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_14 PF23598 876 - 996 1.2e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 1185 - 1273 7.4e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3413
AbsI CCTCGAGG 1 cut(s) 1670
Acc36I ACCTGC 1 cut(s) 1493
AccB1I GGYRCC 2 cut(s) 1553, 2585
AccB7I CCANNNNNTGG 2 cut(s) 1940, 3174
AccI GTMKAC 3 cut(s) 602, 3043, 3761
AccII CGCG 1 cut(s) 1017
AccIII TCCGGA 1 cut(s) 1320
AciI CCGC 6 cut(s) 324, 504, 867, 1105, 1733, 1836
AclWI GGATC 3 cut(s) 2902, 2928, 3343
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 6 cut(s) 1159, 1884, 2643, 2945, 3141, 3696
AcuI CTGAAG 4 cut(s) 68, 431, 1730, 3324
AcyI GRCGYC 1 cut(s) 1054
AfaI GTAC 4 cut(s) 2242, 2380, 2434, 3275
AfiI CCNNNNNNNGG 4 cut(s) 1940, 2057, 2233, 3174
AflII CTTAAG 2 cut(s) 1736, 2020
AhlI ACTAGT 1 cut(s) 2972
AjiI CACGTC 1 cut(s) 3001
AjnI CCWGG 5 cut(s) 1056, 1232, 1343, 1573, 2286
AjuI GAANNNNNNNTTGG 2 cut(s) 954, 986
AloI GAACNNNNNNTCC 4 cut(s) 1002, 1034, 3170, 3202
Alw21I GWGCWC 3 cut(s) 531, 1320, 1404
Alw26I GTCTC 4 cut(s) 35, 129, 1249, 1442
AlwI GGATC 3 cut(s) 2902, 2928, 3343
AlwNI CAGNNNCTG 2 cut(s) 806, 1410
Ama87I CYCGRG 1 cut(s) 1670
Aor13HI TCCGGA 1 cut(s) 1320
AoxI GGCC 3 cut(s) 211, 1942, 2272
ApoI RAATTY 6 cut(s) 1159, 1884, 2643, 2945, 3141, 3696
ArsI GACNNNNNNTTYG 4 cut(s) 1709, 1741, 1966, 1998
Asp700I GAANNNNTTC 4 cut(s) 1084, 2005, 3144, 3699
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 1 cut(s) 2272
AsuC2I CCSGG 1 cut(s) 663
AsuHPI GGTGA 7 cut(s) 263, 482, 697, 1094, 1520, 1817, 2689
AsuII TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
AvaI CYCGRG 1 cut(s) 1670
BaeI ACNNNNGTAYC 2 cut(s) 2864, 2897
BanI GGYRCC 2 cut(s) 1553, 2585
BanII GRGCYC 1 cut(s) 1320
BbsI GAAGAC 2 cut(s) 2127, 3338
Bbv12I GWGCWC 3 cut(s) 531, 1320, 1404
BceAI ACGGC 3 cut(s) 126, 1201, 2397
BcgI CGANNNNNNTGC 2 cut(s) 995, 1029
BciT130I CCWGG 5 cut(s) 1058, 1234, 1345, 1575, 2288
BciVI GTATCC 1 cut(s) 2865
BclI TGATCA 3 cut(s) 942, 982, 1099
BcnI CCSGG 1 cut(s) 663
BcoDI GTCTC 4 cut(s) 35, 129, 1249, 1442
BcuI ACTAGT 1 cut(s) 2972
BfaI CTAG 9 cut(s) 459, 714, 1374, 2207, 2249, 2891, 2973, 3635, 3901
BfmI CTRYAG 1 cut(s) 534
BfrI CTTAAG 2 cut(s) 1736, 2020
BfuAI ACCTGC 1 cut(s) 1493
BfuI GTATCC 1 cut(s) 2865
BglII AGATCT 1 cut(s) 1352
Bme1390I CCNGG 6 cut(s) 663, 1058, 1234, 1345, 1575, 2288
BmeT110I CYCGRG 1 cut(s) 1670
BmgBI CACGTC 1 cut(s) 3001
BmgT120I GGNCC 1 cut(s) 2272
BmiI GGNNCC 4 cut(s) 1555, 2147, 2587, 3508
BmrFI CCNGG 6 cut(s) 663, 1058, 1234, 1345, 1575, 2288
BmrI ACTGGG 3 cut(s) 1131, 3070, 3536
BmsI GCATC 5 cut(s) 965, 1397, 1891, 3467, 3761
BmuI ACTGGG 3 cut(s) 1131, 3070, 3536
BpiI GAAGAC 2 cut(s) 2127, 3338
BpmI CTGGAG 3 cut(s) 59, 2270, 2930
Bpu14I TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
BpuEI CTTGAG 7 cut(s) 218, 1487, 2083, 2585, 2795, 3262, 3356
BpuMI CCSGG 1 cut(s) 663
BsaBI GATNNNNATC 2 cut(s) 319, 1506
BsaHI GRCGYC 1 cut(s) 1054
BsaJI CCNNGG 6 cut(s) 681, 1233, 1343, 3168, 3331, 3510
BsaWI WCCGGW 1 cut(s) 1320
BsaXI ACNNNNNCTCC 2 cut(s) 1714, 1744
Bsc4I CCNNNNNNNGG 4 cut(s) 1940, 2057, 2233, 3174
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 4 cut(s) 42, 1126, 3065, 3531
Bse3DI GCAATG 2 cut(s) 204, 857
Bse8I GATNNNNATC 2 cut(s) 319, 1506
BseAI TCCGGA 1 cut(s) 1320
BseBI CCWGG 5 cut(s) 1058, 1234, 1345, 1575, 2288
BseDI CCNNGG 6 cut(s) 681, 1233, 1343, 3168, 3331, 3510
BseJI GATNNNNATC 2 cut(s) 319, 1506
BseLI CCNNNNNNNGG 4 cut(s) 1940, 2057, 2233, 3174
BseMI GCAATG 2 cut(s) 204, 857
BseMII CTCAG 9 cut(s) 417, 1288, 1417, 1889, 1979, 2048, 2460, 2524, 2719
BseNI ACTGG 4 cut(s) 42, 1126, 3065, 3531
BseYI CCCAGC 3 cut(s) 421, 2612, 3675
BsgI GTGCAG 2 cut(s) 560, 1240
Bsh1236I CGCG 1 cut(s) 1017
BshFI GGCC 3 cut(s) 213, 1944, 2274
BshNI GGYRCC 2 cut(s) 1553, 2585
BsiHKAI GWGCWC 3 cut(s) 531, 1320, 1404
BsiHKCI CYCGRG 1 cut(s) 1670
BsiSI CCGG 4 cut(s) 210, 662, 1321, 2094
BslFI GGGAC 2 cut(s) 1138, 1271
BslI CCNNNNNNNGG 4 cut(s) 1940, 2057, 2233, 3174
BsmAI GTCTC 4 cut(s) 35, 129, 1249, 1442
BsmBI CGTCTC 2 cut(s) 35, 1442
BsmFI GGGAC 2 cut(s) 1138, 1271
BsmI GAATGC 7 cut(s) 740, 2344, 2427, 3376, 3495, 3598, 3866
BsnI GGCC 3 cut(s) 213, 1944, 2274
BsoBI CYCGRG 1 cut(s) 1670
Bsp119I TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
Bsp1286I GDGCHC 3 cut(s) 531, 1320, 1404
Bsp13I TCCGGA 1 cut(s) 1320
Bsp19I CCATGG 1 cut(s) 3331
BspACI CCGC 6 cut(s) 324, 504, 867, 1105, 1733, 1836
BspANI GGCC 3 cut(s) 213, 1944, 2274
BspCNI CTCAG 9 cut(s) 416, 1287, 1416, 1888, 1978, 2047, 2459, 2523, 2718
BspEI TCCGGA 1 cut(s) 1320
BspFNI CGCG 1 cut(s) 1017
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 4 cut(s) 1555, 2147, 2587, 3508
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 1 cut(s) 1493
BspPI GGATC 3 cut(s) 2902, 2928, 3343
BspQI GCTCTTC 1 cut(s) 3300
BspT104I TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
BspT107I GGYRCC 2 cut(s) 1553, 2585
BspTI CTTAAG 2 cut(s) 1736, 2020
BsrDI GCAATG 2 cut(s) 204, 857
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 4 cut(s) 42, 1126, 3065, 3531
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 6 cut(s) 681, 1233, 1343, 3168, 3331, 3510
BssNAI GTATAC 2 cut(s) 3044, 3762
BssNI GRCGYC 1 cut(s) 1054
BssT1I CCWWGG 3 cut(s) 681, 3168, 3331
Bst1107I GTATAC 2 cut(s) 3044, 3762
Bst2UI CCWGG 5 cut(s) 1058, 1234, 1345, 1575, 2288
Bst6I CTCTTC 2 cut(s) 125, 3300
BstACI GRCGYC 1 cut(s) 1054
BstAFI CTTAAG 2 cut(s) 1736, 2020
BstBI TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
BstC8I GCNNGC 7 cut(s) 211, 215, 899, 1046, 1132, 2262, 3087
BstDSI CCRYGG 1 cut(s) 3331
BstFNI CGCG 1 cut(s) 1017
BstHHI GCGC 1 cut(s) 501
BstMAI GTCTC 4 cut(s) 35, 129, 1249, 1442
BstMWI GCNNNNNNNGC 2 cut(s) 898, 2080
BstNI CCWGG 5 cut(s) 1058, 1234, 1345, 1575, 2288
BstNSI RCATGY 3 cut(s) 1048, 1525, 3089
BstSCI CCNGG 6 cut(s) 661, 1056, 1232, 1343, 1573, 2286
BstSFI CTRYAG 1 cut(s) 534
BstUI CGCG 1 cut(s) 1017
BstV2I GAAGAC 2 cut(s) 2127, 3338
BstX2I RGATCY 2 cut(s) 1352, 2920
BstYI RGATCY 2 cut(s) 1352, 2920
BstZ17I GTATAC 2 cut(s) 3044, 3762
BsuI GTATCC 1 cut(s) 2865
BsuRI GGCC 3 cut(s) 213, 1944, 2274
BtgI CCRYGG 1 cut(s) 3331
BtgZI GCGATG 3 cut(s) 709, 998, 2279
BtrI CACGTC 1 cut(s) 3001
BtsI GCAGTG 3 cut(s) 531, 1228, 2768
BtsIMutI CAGTG 6 cut(s) 531, 1194, 1228, 2768, 2987, 3789
BveI ACCTGC 1 cut(s) 1493
Cac8I GCNNGC 7 cut(s) 211, 215, 899, 1046, 1132, 2262, 3087
CaiI CAGNNNCTG 2 cut(s) 806, 1410
CciI TCATGA 1 cut(s) 235
CfoI GCGC 1 cut(s) 501
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 2272
CseI GACGC 4 cut(s) 52, 1062, 1275, 3890
CsiI ACCWGGT 1 cut(s) 1573
Csp6I GTAC 4 cut(s) 2241, 2379, 2433, 3274
CspCI CAANNNNNGTGG 2 cut(s) 2638, 2673
CviQI GTAC 4 cut(s) 2241, 2379, 2433, 3274
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 2 cut(s) 125, 3300
EarI CTCTTC 2 cut(s) 125, 3300
Ecl136II GAGCTC 1 cut(s) 1318
Eco130I CCWWGG 3 cut(s) 681, 3168, 3331
Eco24I GRGCYC 1 cut(s) 1320
Eco32I GATATC 5 cut(s) 321, 1473, 2961, 3094, 3238
Eco53kI GAGCTC 1 cut(s) 1318
Eco57I CTGAAG 4 cut(s) 68, 431, 1730, 3324
Eco88I CYCGRG 1 cut(s) 1670
EcoICRI GAGCTC 1 cut(s) 1318
EcoO109I RGGNCCY 1 cut(s) 2272
EcoRI GAATTC 1 cut(s) 2643
EcoRII CCWGG 5 cut(s) 1056, 1232, 1343, 1573, 2286
EcoRV GATATC 5 cut(s) 321, 1473, 2961, 3094, 3238
EcoT14I CCWWGG 3 cut(s) 681, 3168, 3331
EcoT22I ATGCAT 4 cut(s) 980, 2429, 2634, 3015
EcoT38I GRGCYC 1 cut(s) 1320
ErhI CCWWGG 3 cut(s) 681, 3168, 3331
Esp3I CGTCTC 2 cut(s) 35, 1442
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 2 cut(s) 1138, 1271
FauNDI CATATG 2 cut(s) 2139, 2628
FbaI TGATCA 3 cut(s) 942, 982, 1099
FblI GTMKAC 3 cut(s) 602, 3043, 3761
FriOI GRGCYC 1 cut(s) 1320
FspBI CTAG 9 cut(s) 459, 714, 1374, 2207, 2249, 2891, 2973, 3635, 3901
GlaI GCGC 1 cut(s) 500
GsaI CCCAGC 3 cut(s) 425, 2616, 3679
GsuI CTGGAG 3 cut(s) 59, 2270, 2930
HaeIII GGCC 3 cut(s) 213, 1944, 2274
HapII CCGG 4 cut(s) 210, 662, 1321, 2094
HgaI GACGC 4 cut(s) 52, 1062, 1275, 3890
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 1054
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HincII GTYRAC 6 cut(s) 2212, 2602, 3058, 3400, 3810, 3898
HindII GTYRAC 6 cut(s) 2212, 2602, 3058, 3400, 3810, 3898
HindIII AAGCTT 4 cut(s) 305, 617, 1648, 1840
HpaI GTTAAC 1 cut(s) 2212
HpaII CCGG 4 cut(s) 210, 662, 1321, 2094
HphI GGTGA 7 cut(s) 263, 482, 697, 1094, 1520, 1817, 2689
Hpy99I CGWCG 1 cut(s) 2737
HpyCH4IV ACGT 6 cut(s) 104, 588, 2367, 3000, 3441, 3450
HpyF10VI GCNNNNNNNGC 2 cut(s) 898, 2080
HpySE526I ACGT 6 cut(s) 104, 588, 2367, 3000, 3441, 3450
Hsp92I GRCGYC 1 cut(s) 1054
HspAI GCGC 1 cut(s) 499
Kpn2I TCCGGA 1 cut(s) 1320
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 3 cut(s) 942, 982, 1099
KspAI GTTAAC 1 cut(s) 2212
LguI GCTCTTC 1 cut(s) 3300
LweI GCATC 5 cut(s) 965, 1397, 1891, 3467, 3761
MabI ACCWGGT 1 cut(s) 1573
MaeI CTAG 9 cut(s) 459, 714, 1374, 2207, 2249, 2891, 2973, 3635, 3901
MaeII ACGT 6 cut(s) 104, 588, 2367, 3000, 3441, 3450
MaeIII GTNAC 5 cut(s) 61, 251, 470, 3101, 3208
MfeI CAATTG 4 cut(s) 69, 596, 1631, 2802
MflI RGATCY 2 cut(s) 1352, 2920
MhlI GDGCHC 3 cut(s) 531, 1320, 1404
MlyI GAGTC 7 cut(s) 620, 650, 1963, 2277, 2444, 2688, 2818
MmeI TCCRAC 7 cut(s) 678, 952, 2226, 3007, 3405, 3446, 3774
Mph1103I ATGCAT 4 cut(s) 980, 2429, 2634, 3015
MroI TCCGGA 1 cut(s) 1320
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 4 cut(s) 1084, 2005, 3144, 3699
MslI CAYNNNNRTG 4 cut(s) 1197, 1520, 2337, 2631
MspA1I CMGCKG 3 cut(s) 806, 1107, 2887
MspCI CTTAAG 2 cut(s) 1736, 2020
MspI CCGG 4 cut(s) 210, 662, 1321, 2094
MspR9I CCNGG 6 cut(s) 663, 1058, 1234, 1345, 1575, 2288
MunI CAATTG 4 cut(s) 69, 596, 1631, 2802
Mva1269I GAATGC 7 cut(s) 740, 2344, 2427, 3376, 3495, 3598, 3866
MvaI CCWGG 5 cut(s) 1058, 1234, 1345, 1575, 2288
MvnI CGCG 1 cut(s) 1017
MwoI GCNNNNNNNGC 2 cut(s) 898, 2080
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 663
NcoI CCATGG 1 cut(s) 3331
NdeI CATATG 2 cut(s) 2139, 2628
NgoMIV GCCGGC 1 cut(s) 209
NlaIV GGNNCC 4 cut(s) 1555, 2147, 2587, 3508
NmeAIII GCCGAG 2 cut(s) 1795, 3535
NmuCI GTSAC 3 cut(s) 251, 470, 3101
NsiI ATGCAT 4 cut(s) 980, 2429, 2634, 3015
NspI RCATGY 3 cut(s) 1048, 1525, 3089
NspV TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
PaeI GCATGC 2 cut(s) 1048, 3089
PaeR7I CTCGAG 1 cut(s) 1670
PagI TCATGA 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 3300
PcsI WCGNNNNNNNCGW 2 cut(s) 3447, 3563
PctI GAATGC 7 cut(s) 740, 2344, 2427, 3376, 3495, 3598, 3866
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 4 cut(s) 1084, 2005, 3144, 3699
PfeI GAWTC 8 cut(s) 1080, 1339, 2359, 2490, 2661, 2779, 3544, 3616
PflMI CCANNNNNTGG 2 cut(s) 1940, 3174
PleI GAGTC 7 cut(s) 619, 650, 1963, 2277, 2444, 2688, 2817
PpsI GAGTC 7 cut(s) 619, 650, 1963, 2277, 2444, 2688, 2817
PsiI TTATAA 1 cut(s) 3413
Psp124BI GAGCTC 1 cut(s) 1320
Psp6I CCWGG 5 cut(s) 1056, 1232, 1343, 1573, 2286
PspFI CCCAGC 3 cut(s) 421, 2612, 3675
PspGI CCWGG 5 cut(s) 1056, 1232, 1343, 1573, 2286
PspN4I GGNNCC 4 cut(s) 1555, 2147, 2587, 3508
PspPI GGNCC 1 cut(s) 2272
PspXI VCTCGAGB 1 cut(s) 1670
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 2 cut(s) 806, 1410
PsuI RGATCY 2 cut(s) 1352, 2920
PvuII CAGCTG 2 cut(s) 806, 2887
RsaI GTAC 4 cut(s) 2242, 2380, 2434, 3275
RsaNI GTAC 4 cut(s) 2241, 2379, 2433, 3274
RseI CAYNNNNRTG 4 cut(s) 1197, 1520, 2337, 2631
SacI GAGCTC 1 cut(s) 1320
SapI GCTCTTC 1 cut(s) 3300
Sau96I GGNCC 1 cut(s) 2272
SchI GAGTC 7 cut(s) 620, 650, 1963, 2277, 2444, 2688, 2818
ScrFI CCNGG 6 cut(s) 663, 1058, 1234, 1345, 1575, 2288
SduI GDGCHC 3 cut(s) 531, 1320, 1404
SexAI ACCWGGT 1 cut(s) 1573
SfaNI GCATC 5 cut(s) 965, 1397, 1891, 3467, 3761
SfcI CTRYAG 1 cut(s) 534
Sfr274I CTCGAG 1 cut(s) 1670
SfuI TTCGAA 4 cut(s) 1534, 2168, 3360, 3557
SlaI CTCGAG 1 cut(s) 1670
SmiMI CAYNNNNRTG 4 cut(s) 1197, 1520, 2337, 2631
SpeI ACTAGT 1 cut(s) 2972
SphI GCATGC 2 cut(s) 1048, 3089
SsiI CCGC 6 cut(s) 324, 504, 867, 1105, 1733, 1836
SspI AATATT 2 cut(s) 347, 1298
SspMI CTAG 9 cut(s) 459, 714, 1374, 2207, 2249, 2891, 2973, 3635, 3901
SstI GAGCTC 1 cut(s) 1320
StyD4I CCNGG 6 cut(s) 661, 1056, 1232, 1343, 1573, 2286
StyI CCWWGG 3 cut(s) 681, 3168, 3331
TaiI ACGT 6 cut(s) 107, 591, 2370, 3003, 3444, 3453
TaqII GACCGA 1 cut(s) 3306
TatI WGTACW 1 cut(s) 3273
TfiI GAWTC 8 cut(s) 1080, 1339, 2359, 2490, 2661, 2779, 3544, 3616
TscAI CASTG 6 cut(s) 538, 1201, 1228, 2775, 2987, 3789
TseFI GTSAC 3 cut(s) 251, 470, 3101
Tsp45I GTSAC 3 cut(s) 251, 470, 3101
TspDTI ATGAA 9 cut(s) 143, 224, 1676, 2415, 2954, 3081, 3270, 3470, 3692
TspGWI ACGGA 3 cut(s) 37, 2884, 3152
TspRI CASTG 6 cut(s) 538, 1201, 1228, 2775, 2987, 3789
Van91I CCANNNNNTGG 2 cut(s) 1940, 3174
Vha464I CTTAAG 2 cut(s) 1736, 2020
XapI RAATTY 6 cut(s) 1159, 1884, 2643, 2945, 3141, 3696
XbaI TCTAGA 2 cut(s) 2248, 3634
XceI RCATGY 3 cut(s) 1048, 1525, 3089
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XhoI CTCGAG 1 cut(s) 1670
XmiI GTMKAC 3 cut(s) 602, 3043, 3761
XmnI GAANNNNTTC 4 cut(s) 1084, 2005, 3144, 3699
XspI CTAG 9 cut(s) 459, 714, 1374, 2207, 2249, 2891, 2973, 3635, 3901
Zsp2I ATGCAT 4 cut(s) 980, 2429, 2634, 3015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.