Rh3CG255700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0009232
Physical Location & Seq
Reverse (-)
24964283 .. 24981164
16882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG255700.1

Sequence Viewer

Length: 3846 bp
ATGGCCTTAGGTACAAAGGAGGAACATATAAAGAAGATATTGGAGCTGTTGAAGGATCCCTATGTGCCAACAGTAGTTCTTGATGGGAAGCGTGGAGTAGGGAAAACGTGGATAGCAAAAGAGATAATCAAGGATAAAGATTTAATCCATGACACTCTCTGGCTGTATGTGAATAACAATTATGACACACAATCTTTTCACGAAAACATTGCTCGCCAGCTATCTCTATTCTCTATTTATGAGGAATGGGAAGATGATGATGCTGCTGAGGAGGAACAGAGATTGGAGAAGTTGATGCCGAGAATATCTGAGAAGCTTGAAGAGTTCAGATCTGCAGCACATGAAGAAAAGAAACTATTTCTTCTTATTCTGGATGATCTTCCTCATCAGAAGGATGTAGAGGAGATTGTGAACGAAGTTAACAACCTGCTGACTTCAAATGAATCTTTCAAGGTTTTAATCACCAGAGGCGAAAGCGATGGAAGAGGCACCACGCTGGGGATGAACCAGGAAATTAAGAGCTGCAATACAACAGTATACCCGATAGATCCATTGTCCACTGATGAGTCCTCAGCTTTATTGCATGAAAAAATCAAGAAAGAAGTATTTGATTGTCCAGGCTTTGGAAAACTATCCAACGCTATTGTGGAGATGAGTAAGGGAATTCCTACTATACTTATCACGACAGCAAAAGCAATAAATTACTTGGCAGATAAAGGTTCCGAGGTTTGGTCGTTGGAAAATATTTTGGAAGAAGCGGCTTATGATGGGGAAACTGCTATAAATTTGCTACTTCGCTCTTGGCGTAATGCATTGCCAACCGATTCTCTGACAGACTGCTTTTGGCATACCATGCAGCTTCTAAACAAACATGGTGGTGTTCATTACAATGAGTTGATCACACAATGGATAATGGAAGGATATTTTGGATGCAACAATCAGATTGGGAAGGTCTATGAGCAAGGACATAAGGTCCTGATGGACCTTATAGATTGGGGCATGCTGAGAAAACAAGAGGATAACATGGTTGTAATGGGAGGAATAATTTTGTGTATAACTGGTCATTATCGACGTGAATTGAATGCGACTGCAAGGCTCGGAATGGCTAATGTGTTTGAAGATGAAAATTGGAAAGGTCTTGGAATGCTTGCACATACGGATCGTATGATAAAATCACCATCCAGTCATAAGACCTGGGAGAAAGTTTCCACACTTCTGATGGATGGCCAGTGCATCGATAGGGAAGTACCTGTAACATGCTTTCAACGCATGGAGACTCTCCAAGTTCTCATGTTAATCAAGCCTAGGTTCACATCTCTGCCAGTATCCTTAGTTGACATAAAGAAACTTTCCATTCTTGTGCTCAGATTCTGTGATCGCTTGGAGAAGATTGATCCTATCCAAGAATTTCAAAGCTTAAGTGTTCTTGAGATATCTGGTGCTACTTCGTTGAAGATGATCCCTGATAACTTTTTTGAAACGATGAGTAACCTTCGGAGCCTTAACCTTTCTGAAGCCAATGTCAAATCACTACCTAGTTCTCTTTTCAACAGAAGTGAACTACGGTGGCTCATTCTTAGAGGATGTTCTCAATTGAAAACACTTCCAAGCTTAAAATCCTTGGGAAAGCTCAAGGTGCTTGATCTTTCTGGTGCTACCTCTTTCAGTAGATTCCGAGACAAAACCTTTGCAGCTCTTCAGAAACTTCATACACTTAATCTTTCTAATTCCCAAATTACTCGCTTACCCTTTCTTCATGGTAATGATGAGCTTAATCAGCTCCTATTAGGTGGTTGTGCAAGTTTAAACAGACTTCCAACCTTGAACACAATACCTCGGCTTCAAATTCTTGATCTTTCTGGTGCTACAGGCTTAAAAGAAATACAGTTTGAACCTTTGGATACCCTTAAGGCCCTTGATTTATCTGGAACTCTTATTAAAAGGCAACCTTCCAGTTTCAGCCACCTTTCTGATCTCTCCTTACGGGAGTGTCGTGAGCTTGTAAAACTTCCACTCACAAAAGAAATCAAATATCTTGAATCACTTGACCTTTCTGGTTCTAGCACCATCGTTGAAATTGAAGACAAATTCTTCCAGCAAATGAGGTTTCTTCGAGTTCTGAACTTTTCAACAGTTAAGATAAAAGAACTACCTTCCCTGTCCAACCTAGCTAACCTCTGTCGACTTTTGCTAATGGATTGTTCACGTTTAGAAAAGCTTCCAGAGATGGAAGGACTTAAAAGGCTCCAAGAGCTTAATCTTTCTGGTTGTGTGGCATTGGTTGATTTGCCAAACCTGAACTCCCTTGAAAAACTGGAGATTCTTGATATTTCAGGCTGCAGGGTTTTGAAACAAATACAAGAGGAATCCTTTCAAAAGATGTTCCATATTCGGATACTCAACCTCTCAGACACTATGATTGAGTTCCTTCCATCCATTTGCAAACCCAGCAACCTCTGTCACCTCATCCTCAGAAATTGCAAACACTTAAAAAAGCTTCCACCTCTTGAGCATTTGTCAAAACTTGAGGAACTGAATCTGTGTGGTGCCAGTTCTTTAGTTGACTTTACATCTGAATTCCTGGAGCATATGGTCTACCTTCAAATTGTTGACTTATCTGAAATCCCATTGACAACGGTACCATCCATGTCCAAATTCATCAAACTCAGACAGTTCTCAGTAAAGGGTTGTTCATCCTTAAATGAAGTGCTACATCTTGAAGTACTAACACTTCTTGAGATTCTTGATCTTTCTGGAACAGCAGTTCCGAGTCTGCCTCCCCTAAACACTTTTAGCAACCTCCACCAGCTCTTGCTAAAAGATTGTTCACAAATAAAAGAGTTGCAGTTTCTAGAGTCGCTTAGTAGGCTGGAGGTTCTTGATCTGTCAGGAACCAGAATGAAGCAATTTCCTTATGAGATATCAGAGCTTACTCATTTAAAGCAGCTTTATCTGCCAGACTTGAGGGGTATGTCTGATCTTGACTTGGGAAAGATAAAGCGTTTGCCACAAGAGGTCAACTGGGATGAATGTGGCATCTTTGAGTGTGCTGATATTTACGTAGGCAGTGACAAGATTTCCCTATCTGTAAGTGGCTTTGAAATTTTTCACATTTTGGACAAACATCCAAAGTTTCGGGAAGAAAAGTTCAAAAAATTTCATTTCCATGTAAAGAATAAGGAAGGTGAAGATGGAGGTATCAAGTACTGCAAAGATGAATTTATCTTCAGAGATGTGTACTTCCACACTAGAAATTTTTATCCTAAAGAGCACGATAGGTCTTTGGAAATCTATGGATCATATAGTCTCCCTAAGGGTTTTGAAAGTGTCCTCAAGTGTGCCGAATGCTTATCTTTGGTTGACAATGACCTTATACATAGCCTATCTGATTTAGGTTCTGACAATGTGAAGACAATGAAAGGCTGTTGGTTAGAGAGGTGTAGTAAAGTACATAACATTCTTTCTAATGAAGAGGCAGGTGTCACACTGGGCAGATGTTTAGAGACTCTATGGATTTCAAACCTTCCAATTTTGAGCAGTCTATACAATGGGAGTGTGCAATCAGTGGTCTTTCAGAACCTTAAACAATTGTATCTGGATTGTTGTCCAGGCATCATAAATGTCTTTCCATCCTCCCAGCTGCCAAAAAACCTGGAAAATCTACATGTTCAGTTTTGTGATAAGTTGCAAACTTTGTTTGAATGTGATTCACAATCTAGTTGTGCAATGCAAAGGTTGAGGAAATTGTATCTACTGGAACTACCGGAGTTGTGGAGCATTGGGATAAAATTGCCAGCTGAACTGACTTTTGTGGTCATCGAATGCCCCAAGTTGCTGAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_8 NB-ARC

Protein Analysis

1281

Amino Acids

145.95

Weight (kDa)

5.81

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 7 - 140 2e-07 NB-ARC domain
ATPase_2 PF01637 7 - 224 7.3e-07 ATPase domain predominantly from Archaea
LRR_14 PF23598 462 - 578 2.6e-07 Leucine-rich repeat region
LRR_8 PF13855 472 - 529 7.4e-06 Leucine rich repeat
LRR_EndoS PF23952 702 - 786 2.4e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_14 PF23598 762 - 906 3e-07 Leucine-rich repeat region
LRR_14 PF23598 919 - 1004 1.7e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 1124 - 1212 8.9e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3467
Acc36I ACCTGC 2 cut(s) 435, 3467
Acc65I GGTACC 1 cut(s) 2638
AccB1I GGYRCC 3 cut(s) 488, 2546, 2638
AccB7I CCANNNNNTGG 1 cut(s) 623
AccI GTMKAC 3 cut(s) 537, 2182, 2595
AciI CCGC 1 cut(s) 758
AclWI GGATC 7 cut(s) 50, 63, 542, 1167, 1388, 1453, 3304
AcoI YGGCCR 1 cut(s) 1225
AcuI CTGAAG 3 cut(s) 1533, 1682, 3211
AfaI GTAC 7 cut(s) 13, 1248, 2640, 2724, 3206, 3239, 3450
AfiI CCNNNNNNNGG 3 cut(s) 498, 623, 729
AflII CTTAAG 2 cut(s) 1417, 1907
AflIII ACRYGT 1 cut(s) 3664
AhdI GACNNNNNGTC 1 cut(s) 971
AjiI CACGTC 1 cut(s) 1073
AjnI CCWGG 6 cut(s) 507, 616, 1193, 2580, 3607, 3651
AjuI GAANNNNNNNTTGG 2 cut(s) 909, 941
AloI GAACNNNNNNTCC 6 cut(s) 2748, 2749, 2780, 2781, 3131, 3163
Alw21I GWGCWC 2 cut(s) 1365, 3273
Alw26I GTCTC 4 cut(s) 1268, 1671, 3311, 3497
AlwI GGATC 7 cut(s) 50, 63, 542, 1167, 1388, 1453, 3304
AlwNI CAGNNNCTG 1 cut(s) 1371
AoxI GGCC 3 cut(s) 3, 1225, 1911
ApeKI GCWGC 8 cut(s) 263, 335, 522, 856, 1691, 2337, 2944, 3640
ArsI GACNNNNNNTTYG 2 cut(s) 1670, 1702
Asp700I GAANNNNTTC 3 cut(s) 2217, 2370, 3105
Asp718I GGTACC 1 cut(s) 2638
AspA2I CCTAGG 1 cut(s) 1304
AspS9I GGNCC 3 cut(s) 973, 982, 1912
AsuHPI GGTGA 4 cut(s) 454, 1167, 2453, 3197
AvaII GGWCC 2 cut(s) 973, 982
AvrII CCTAGG 1 cut(s) 1304
AxyI CCTNAGG 2 cut(s) 7, 3312
BaeI ACNNNNGTAYC 4 cut(s) 2622, 2655, 3575, 3608
BalI TGGCCA 1 cut(s) 1227
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 3 cut(s) 488, 2546, 2638
BarI GAAGNNNNNNTAC 2 cut(s) 2715, 2747
BbsI GAAGAC 2 cut(s) 2088, 3416
Bbv12I GWGCWC 2 cut(s) 1365, 3273
BbvCI CCTCAGC 2 cut(s) 267, 571
BbvI GCAGC 8 cut(s) 250, 347, 509, 868, 1703, 2324, 2956, 3627
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
BciVI GTATCC 3 cut(s) 1336, 1894, 2388
BclI TGATCA 1 cut(s) 897
BcoDI GTCTC 4 cut(s) 1268, 1671, 3311, 3497
BfaI CTAG 7 cut(s) 1305, 1536, 2061, 2168, 2852, 3249, 3717
BfmI CTRYAG 4 cut(s) 333, 1866, 2338, 3842
BfrI CTTAAG 2 cut(s) 1417, 1907
BfuAI ACCTGC 2 cut(s) 435, 3467
BfuI GTATCC 3 cut(s) 1336, 1894, 2388
BglII AGATCT 1 cut(s) 329
BisI GCNGC 9 cut(s) 264, 336, 523, 759, 857, 1692, 2338, 2945, 3641
BlnI CCTAGG 1 cut(s) 1304
BlsI GCNGC 9 cut(s) 265, 337, 524, 760, 858, 1693, 2339, 2946, 3642
BmcAI AGTACT 2 cut(s) 2724, 3206
Bme1390I CCNGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
Bme18I GGWCC 2 cut(s) 973, 982
BmeRI GACNNNNNGTC 1 cut(s) 971
BmgBI CACGTC 1 cut(s) 1073
BmgT120I GGNCC 3 cut(s) 973, 982, 1912
BmiI GGNNCC 8 cut(s) 57, 490, 721, 1499, 2246, 2548, 2640, 2893
BmrFI CCNGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
BmrI ACTGGG 2 cut(s) 3031, 3497
BmsI GCATC 6 cut(s) 250, 285, 920, 1242, 3045, 3621
BmuI ACTGGG 2 cut(s) 3031, 3497
BpiI GAAGAC 2 cut(s) 2088, 3416
BplI GAGNNNNNCTC 2 cut(s) 2761, 2793
BpmI CTGGAG 3 cut(s) 2336, 2603, 2891
Bpu10I CCTNAGC 2 cut(s) 267, 571
BpuEI CTTGAG 7 cut(s) 1448, 1616, 2528, 2546, 2756, 2983, 3317
Bsa29I ATCGAT 1 cut(s) 1236
BsaAI YACGTR 1 cut(s) 3061
BsaJI CCNNGG 5 cut(s) 723, 1194, 1304, 1620, 1835
BsaWI WCCGGW 1 cut(s) 3763
BsaXI ACNNNNNCTCC 2 cut(s) 2285, 2315
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 623, 729
Bse21I CCTNAGG 2 cut(s) 7, 3312
Bse3DI GCAATG 3 cut(s) 207, 812, 3732
BseBI CCWGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
BseCI ATCGAT 1 cut(s) 1236
BseDI CCNNGG 5 cut(s) 723, 1194, 1304, 1620, 1835
BseLI CCNNNNNNNGG 3 cut(s) 498, 623, 729
BseMI GCAATG 3 cut(s) 207, 812, 3732
BseMII CTCAG 9 cut(s) 258, 300, 585, 995, 1378, 2421, 2485, 2680, 2691
BseRI GAGGAG 2 cut(s) 284, 416
BseXI GCAGC 8 cut(s) 250, 347, 509, 868, 1703, 2324, 2956, 3627
BseYI CCCAGC 3 cut(s) 496, 2447, 3636
BshFI GGCC 3 cut(s) 5, 1227, 1913
BshNI GGYRCC 3 cut(s) 488, 2546, 2638
BshVI ATCGAT 1 cut(s) 1236
BsiHKAI GWGCWC 2 cut(s) 1365, 3273
BsiSI CCGG 1 cut(s) 3764
BslI CCNNNNNNNGG 3 cut(s) 498, 623, 729
BsmAI GTCTC 4 cut(s) 1268, 1671, 3311, 3497
BsmI GAATGC 4 cut(s) 1087, 1149, 3350, 3827
BsnI GGCC 3 cut(s) 5, 1227, 1913
Bsp1286I GDGCHC 2 cut(s) 1365, 3273
BspACI CCGC 1 cut(s) 758
BspANI GGCC 3 cut(s) 5, 1227, 1913
BspCNI CTCAG 9 cut(s) 259, 301, 584, 996, 1377, 2420, 2484, 2679, 2690
BspDI ATCGAT 1 cut(s) 1236
BspLI GGNNCC 8 cut(s) 57, 490, 721, 1499, 2246, 2548, 2640, 2893
BspMAI CTGCAG 2 cut(s) 337, 2342
BspMI ACCTGC 2 cut(s) 435, 3467
BspPI GGATC 7 cut(s) 50, 63, 542, 1167, 1388, 1453, 3304
BspQI GCTCTTC 1 cut(s) 1701
BspT107I GGYRCC 3 cut(s) 488, 2546, 2638
BspTI CTTAAG 2 cut(s) 1417, 1907
BsrDI GCAATG 3 cut(s) 207, 812, 3732
BssECI CCNNGG 5 cut(s) 723, 1194, 1304, 1620, 1835
BssNAI GTATAC 1 cut(s) 538
BssT1I CCWWGG 2 cut(s) 1304, 1620
Bst1107I GTATAC 1 cut(s) 538
Bst2UI CCWGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
Bst4CI ACNGT 7 cut(s) 73, 535, 1566, 1887, 2134, 2638, 2673
Bst6I CTCTTC 4 cut(s) 315, 478, 1701, 3465
BstAFI CTTAAG 2 cut(s) 1417, 1907
BstAPI GCANNNNNTGC 1 cut(s) 853
BstBAI YACGTR 1 cut(s) 3061
BstC8I GCNNGC 5 cut(s) 214, 218, 1001, 1149, 3795
BstMAI GTCTC 4 cut(s) 1268, 1671, 3311, 3497
BstMWI GCNNNNNNNGC 8 cut(s) 853, 1266, 1636, 1777, 2251, 2448, 2866, 2953
BstNI CCWGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
BstNSI RCATGY 3 cut(s) 1003, 1260, 3668
BstSCI CCNGG 6 cut(s) 507, 616, 1193, 2580, 3607, 3651
BstSFI CTRYAG 4 cut(s) 333, 1866, 2338, 3842
BstSNI TACGTA 1 cut(s) 3061
BstV1I GCAGC 8 cut(s) 250, 347, 509, 868, 1703, 2324, 2956, 3627
BstV2I GAAGAC 2 cut(s) 2088, 3416
BstX2I RGATCY 3 cut(s) 55, 329, 547
BstYI RGATCY 3 cut(s) 55, 329, 547
BstZ17I GTATAC 1 cut(s) 538
Bsu15I ATCGAT 1 cut(s) 1236
Bsu36I CCTNAGG 2 cut(s) 7, 3312
BsuI GTATCC 3 cut(s) 1336, 1894, 2388
BsuRI GGCC 3 cut(s) 5, 1227, 1913
BsuTUI ATCGAT 1 cut(s) 1236
BtgZI GCGATG 1 cut(s) 492
BtrI CACGTC 1 cut(s) 1073
BtsI GCAGTG 1 cut(s) 3073
BtsIMutI CAGTG 5 cut(s) 558, 1235, 3073, 3485, 3570
BveI ACCTGC 2 cut(s) 435, 3467
Cac8I GCNNGC 5 cut(s) 214, 218, 1001, 1149, 3795
CaiI CAGNNNCTG 1 cut(s) 1371
Cfr13I GGNCC 3 cut(s) 973, 982, 1912
ClaI ATCGAT 1 cut(s) 1236
Csp6I GTAC 7 cut(s) 12, 1247, 2639, 2723, 3205, 3238, 3449
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviQI GTAC 7 cut(s) 12, 1247, 2639, 2723, 3205, 3238, 3449
DraI TTTAAA 2 cut(s) 1806, 2940
DriI GACNNNNNGTC 1 cut(s) 971
EaeI YGGCCR 1 cut(s) 1225
Eam1104I CTCTTC 4 cut(s) 315, 478, 1701, 3465
Eam1105I GACNNNNNGTC 1 cut(s) 971
EarI CTCTTC 4 cut(s) 315, 478, 1701, 3465
Eco105I TACGTA 1 cut(s) 3061
Eco130I CCWWGG 2 cut(s) 1304, 1620
Eco32I GATATC 2 cut(s) 1434, 2922
Eco47I GGWCC 2 cut(s) 973, 982
Eco57I CTGAAG 3 cut(s) 1533, 1682, 3211
Eco81I CCTNAGG 2 cut(s) 7, 3312
EcoO109I RGGNCCY 2 cut(s) 973, 1912
EcoRI GAATTC 2 cut(s) 663, 2576
EcoRII CCWGG 6 cut(s) 507, 616, 1193, 2580, 3607, 3651
EcoRV GATATC 2 cut(s) 1434, 2922
EcoT14I CCWWGG 2 cut(s) 1304, 1620
EcoT22I ATGCAT 1 cut(s) 814
ErhI CCWWGG 2 cut(s) 1304, 1620
FalI AAGNNNNNCTT 2 cut(s) 1933, 1965
FauNDI CATATG 1 cut(s) 2589
FbaI TGATCA 1 cut(s) 897
FblI GTMKAC 3 cut(s) 537, 2182, 2595
Fnu4HI GCNGC 9 cut(s) 264, 336, 523, 759, 857, 1692, 2338, 2945, 3641
Fsp4HI GCNGC 9 cut(s) 264, 336, 523, 759, 857, 1692, 2338, 2945, 3641
FspBI CTAG 7 cut(s) 1305, 1536, 2061, 2168, 2852, 3249, 3717
GluI GCNGC 9 cut(s) 264, 336, 523, 759, 857, 1692, 2338, 2945, 3641
GsaI CCCAGC 3 cut(s) 500, 2451, 3640
GsuI CTGGAG 3 cut(s) 2336, 2603, 2891
HaeIII GGCC 3 cut(s) 5, 1227, 1913
HapII CCGG 1 cut(s) 3764
HincII GTYRAC 7 cut(s) 421, 1336, 2183, 2563, 2611, 3019, 3361
HindII GTYRAC 7 cut(s) 421, 1336, 2183, 2563, 2611, 3019, 3361
HindIII AAGCTT 5 cut(s) 314, 1414, 1609, 2216, 2495
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 1 cut(s) 3764
HphI GGTGA 4 cut(s) 454, 1167, 2453, 3197
Hpy99I CGWCG 1 cut(s) 1074
HpyCH4III ACNGT 7 cut(s) 73, 535, 1566, 1887, 2134, 2638, 2673
HpyCH4IV ACGT 4 cut(s) 107, 1072, 2206, 3060
HpyF10VI GCNNNNNNNGC 8 cut(s) 853, 1266, 1636, 1777, 2251, 2448, 2866, 2953
HpySE526I ACGT 4 cut(s) 107, 1072, 2206, 3060
KpnI GGTACC 1 cut(s) 2642
Ksp22I TGATCA 1 cut(s) 897
KspAI GTTAAC 1 cut(s) 421
LguI GCTCTTC 1 cut(s) 1701
LmnI GCTCC 6 cut(s) 43, 1497, 1785, 2250, 2584, 3774
Lsp1109I GCAGC 8 cut(s) 250, 347, 509, 868, 1703, 2324, 2956, 3627
LweI GCATC 6 cut(s) 250, 285, 920, 1242, 3045, 3621
MaeI CTAG 7 cut(s) 1305, 1536, 2061, 2168, 2852, 3249, 3717
MaeII ACGT 4 cut(s) 107, 1072, 2206, 3060
MaeIII GTNAC 5 cut(s) 1252, 1487, 2459, 3068, 3481
MfeI CAATTG 2 cut(s) 1592, 3587
MflI RGATCY 3 cut(s) 55, 329, 547
MhlI GDGCHC 2 cut(s) 1365, 3273
MlsI TGGCCA 1 cut(s) 1227
MluNI TGGCCA 1 cut(s) 1227
MlyI GAGTC 5 cut(s) 575, 1270, 2779, 2864, 3499
MmeI TCCRAC 4 cut(s) 660, 717, 1841, 2187
Mox20I TGGCCA 1 cut(s) 1227
Mph1103I ATGCAT 1 cut(s) 814
MroXI GAANNNNTTC 3 cut(s) 2217, 2370, 3105
MscI TGGCCA 1 cut(s) 1227
MslI CAYNNNNRTG 5 cut(s) 876, 888, 1358, 1761, 3165
Msp20I TGGCCA 1 cut(s) 1227
MspA1I CMGCKG 2 cut(s) 3640, 3797
MspCI CTTAAG 2 cut(s) 1417, 1907
MspI CCGG 1 cut(s) 3764
MspR9I CCNGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
MssI GTTTAAAC 1 cut(s) 1806
MunI CAATTG 2 cut(s) 1592, 3587
Mva1269I GAATGC 4 cut(s) 1087, 1149, 3350, 3827
MvaI CCWGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
MwoI GCNNNNNNNGC 8 cut(s) 853, 1266, 1636, 1777, 2251, 2448, 2866, 2953
NdeI CATATG 1 cut(s) 2589
NlaIV GGNNCC 8 cut(s) 57, 490, 721, 1499, 2246, 2548, 2640, 2893
NmeAIII GCCGAG 2 cut(s) 324, 1816
NmuCI GTSAC 3 cut(s) 2459, 3068, 3481
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 3 cut(s) 1003, 1260, 3668
PaeI GCATGC 1 cut(s) 1003
PaqCI CACCTGC 1 cut(s) 3467
PciI ACATGT 1 cut(s) 3664
PciSI GCTCTTC 1 cut(s) 1701
PcsI WCGNNNNNNNCGW 2 cut(s) 802, 1990
PctI GAATGC 4 cut(s) 1087, 1149, 3350, 3827
PdmI GAANNNNTTC 3 cut(s) 2217, 2370, 3105
PflMI CCANNNNNTGG 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 2580
PkrI GCNGC 9 cut(s) 265, 337, 524, 760, 858, 1693, 2339, 2946, 3642
PleI GAGTC 5 cut(s) 574, 1270, 2778, 2863, 3499
PmeI GTTTAAAC 1 cut(s) 1806
PpsI GAGTC 5 cut(s) 574, 1270, 2778, 2863, 3499
Ppu21I YACGTR 1 cut(s) 3061
PpuMI RGGWCCY 1 cut(s) 973
PscI ACATGT 1 cut(s) 3664
Psp5II RGGWCCY 1 cut(s) 973
Psp6I CCWGG 6 cut(s) 507, 616, 1193, 2580, 3607, 3651
PspFI CCCAGC 3 cut(s) 496, 2447, 3636
PspGI CCWGG 6 cut(s) 507, 616, 1193, 2580, 3607, 3651
PspN4I GGNNCC 8 cut(s) 57, 490, 721, 1499, 2246, 2548, 2640, 2893
PspPI GGNCC 3 cut(s) 973, 982, 1912
PspPPI RGGWCCY 1 cut(s) 973
PstI CTGCAG 2 cut(s) 337, 2342
PstNI CAGNNNCTG 1 cut(s) 1371
PsuI RGATCY 3 cut(s) 55, 329, 547
PvuII CAGCTG 2 cut(s) 3640, 3797
RsaI GTAC 7 cut(s) 13, 1248, 2640, 2724, 3206, 3239, 3450
RsaNI GTAC 7 cut(s) 12, 1247, 2639, 2723, 3205, 3238, 3449
RseI CAYNNNNRTG 5 cut(s) 876, 888, 1358, 1761, 3165
SalI GTCGAC 1 cut(s) 2181
SapI GCTCTTC 1 cut(s) 1701
SatI GCNGC 9 cut(s) 264, 336, 523, 759, 857, 1692, 2338, 2945, 3641
Sau96I GGNCC 3 cut(s) 973, 982, 1912
ScaI AGTACT 2 cut(s) 2724, 3206
SchI GAGTC 5 cut(s) 575, 1270, 2779, 2864, 3499
ScrFI CCNGG 6 cut(s) 509, 618, 1195, 2582, 3609, 3653
SduI GDGCHC 2 cut(s) 1365, 3273
SfaNI GCATC 6 cut(s) 250, 285, 920, 1242, 3045, 3621
SfcI CTRYAG 4 cut(s) 333, 1866, 2338, 3842
SinI GGWCC 2 cut(s) 973, 982
SmiMI CAYNNNNRTG 5 cut(s) 876, 888, 1358, 1761, 3165
SmlI CTYRAG 9 cut(s) 1417, 1427, 1631, 1907, 2507, 2525, 2735, 2962, 3332
SmoI CTYRAG 9 cut(s) 1417, 1427, 1631, 1907, 2507, 2525, 2735, 2962, 3332
SnaBI TACGTA 1 cut(s) 3061
SphI GCATGC 1 cut(s) 1003
SsiI CCGC 1 cut(s) 758
SspI AATATT 1 cut(s) 745
SspMI CTAG 7 cut(s) 1305, 1536, 2061, 2168, 2852, 3249, 3717
StyD4I CCNGG 6 cut(s) 507, 616, 1193, 2580, 3607, 3651
StyI CCWWGG 2 cut(s) 1304, 1620
TaaI ACNGT 7 cut(s) 73, 535, 1566, 1887, 2134, 2638, 2673
TaiI ACGT 4 cut(s) 110, 1075, 2209, 3063
TaqI TCGA 5 cut(s) 1069, 1236, 2113, 2182, 3819
TatI WGTACW 4 cut(s) 2722, 3204, 3237, 3448
TauI GCSGC 1 cut(s) 761
TscAI CASTG 5 cut(s) 565, 1235, 3073, 3492, 3570
TseFI GTSAC 3 cut(s) 2459, 3068, 3481
TseI GCWGC 8 cut(s) 263, 335, 522, 856, 1691, 2337, 2944, 3640
Tsp45I GTSAC 3 cut(s) 2459, 3068, 3481
TspGWI ACGGA 1 cut(s) 1172
TspRI CASTG 5 cut(s) 565, 1235, 3073, 3492, 3570
Van91I CCANNNNNTGG 1 cut(s) 623
Vha464I CTTAAG 2 cut(s) 1417, 1907
VpaK11BI GGWCC 2 cut(s) 973, 982
XbaI TCTAGA 1 cut(s) 2851
XceI RCATGY 3 cut(s) 1003, 1260, 3668
XcmI CCANNNNNNNNNTGG 2 cut(s) 643, 1216
XmaJI CCTAGG 1 cut(s) 1304
XmiI GTMKAC 3 cut(s) 537, 2182, 2595
XmnI GAANNNNTTC 3 cut(s) 2217, 2370, 3105
XspI CTAG 7 cut(s) 1305, 1536, 2061, 2168, 2852, 3249, 3717
ZrmI AGTACT 2 cut(s) 2724, 3206
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.