MD14G1055400.v1.1

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009951
Physical Location & Seq
Forward (+)
5656492 .. 5657150
659 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1055400.v1.1.491

Sequence Viewer

Length: 603 bp
ATGAAGAAGTTGCTTTTACAGCAAGGAACTGATGATCGGTTCAGGGCAGTAGGCATTGTTGGTAGGGAAGGTATAGGAAAAACAACACTTTGCCAGCTCTTATTCAACGAACAAGAAGTGAAAAACACATTCCTTCCGAGGATCTGGGTGTGCATGGCCAGACACCCGGATGACAACGACAATGAGGCTCCGAAAATAGTAATTGTCAAAAGAATGTTGATGCACCTCGGAGTTGGAAAGGAGATGGTCAAGTCCGTTTGTGAGAAGCATGGCCTCGGGGGACTGCTGTATGCTCTTCGCCTGCAATTATTGGGCAAGAGGTATCTGATTGTGCTTGATCATGCCAGGGAGACGGACTCATGGTGTGGACAGCTGGATTCGTGTTTGACTCGTGATAAGGAATGGGATGACGGCCTTGCATTTGGACTCCCAAAAGGGCATGGGGGAAGAGTCATAGTCACAAGTCAGAATGAAGATATAACAAAAATGATGGTCGGGAAGGAAAATATACATCACCTTCTGCCCCTTTCGGATCATGAAAGCTGCTGGGCGATATTCAAAGACGCAGTTGAGGATAAACGGATTCCGTCCAGACATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

201

Amino Acids

22.7

Weight (kDa)

8.15

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 11 - 193 5.5e-17 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19060 AT5G45440 AT5G45490 AT5G45490
fragaria_vesca FvH4_6g22150
malus_domestica MD12G1059800.v1.1 MD14G1055400.v1.1
prunus_persica Prupe.7G078500_v2.0.a1 Prupe.7G078700_v2.0.a1
pyrus_communis pycom14g04570
rosa_chinensis RchiOBHm_Chr3g0479501
rosa_laevigata RLG00000023564
rosa_multiflora Rmu_sc0004732.1_g000011
rosa_roxburghii Rroxscaffold_6G00402200
rosa_rugosa Rorug03G0175800 Rorug03G0175900
rosa_samantha Rh3AG226800 Rh3BG259900 Rh3CG256600 Rh3DG252400
rosa_wichuraiana Rw3G020260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 149, 540
AcoI YGGCCR 1 cut(s) 156
AgsI TTSAA 2 cut(s) 106, 559
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 3 cut(s) 97, 373, 543
AluI AGCT 3 cut(s) 97, 373, 543
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 2 cut(s) 149, 540
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 3 cut(s) 156, 271, 412
ApeKI GCWGC 1 cut(s) 543
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 275
BalI TGGCCA 1 cut(s) 158
BauI CACGAG 1 cut(s) 390
BbvI GCAGC 1 cut(s) 530
BccI CCATC 2 cut(s) 238, 484
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 1 cut(s) 346
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 344
BisI GCNGC 1 cut(s) 544
BlsI GCNGC 1 cut(s) 545
Bme1390I CCNGG 2 cut(s) 167, 346
BmeT110I CYCGRG 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 2 cut(s) 167, 346
BmsI GCATC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 341, 373
BpuMI CCSGG 1 cut(s) 167
BsaJI CCNNGG 4 cut(s) 137, 226, 274, 345
BseBI CCWGG 1 cut(s) 346
BseDI CCNNGG 4 cut(s) 137, 226, 274, 345
BseGI GGATG 2 cut(s) 175, 412
BseXI GCAGC 1 cut(s) 530
BseYI CCCAGC 1 cut(s) 546
BshFI GGCC 3 cut(s) 158, 273, 414
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 167
BslFI GGGAC 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 344
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 3 cut(s) 158, 273, 414
BsoBI CYCGRG 1 cut(s) 275
Bsp143I GATC 4 cut(s) 34, 141, 337, 532
BspANI GGCC 3 cut(s) 158, 273, 414
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 2 cut(s) 149, 540
BspQI GCTCTTC 1 cut(s) 300
BssECI CCNNGG 4 cut(s) 137, 226, 274, 345
BssMI GATC 4 cut(s) 34, 141, 337, 532
BssSI CACGAG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 346
Bst6I CTCTTC 2 cut(s) 300, 442
BstC8I GCNNGC 2 cut(s) 95, 302
BstF5I GGATG 2 cut(s) 175, 412
BstKTI GATC 4 cut(s) 37, 144, 340, 535
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 4 cut(s) 34, 141, 337, 532
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 165, 344
BstV1I GCAGC 1 cut(s) 530
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
BsuRI GGCC 3 cut(s) 158, 273, 414
BtsCI GGATG 2 cut(s) 175, 412
Cac8I GCNNGC 2 cut(s) 95, 302
CciI TCATGA 1 cut(s) 535
CseI GACGC 1 cut(s) 572
CviAII CATG 6 cut(s) 154, 269, 341, 360, 440, 536
CviJI RGCY 7 cut(s) 97, 158, 188, 273, 373, 414, 543
CviKI_1 RGCY 7 cut(s) 97, 158, 188, 273, 373, 414, 543
DpnI GATC 4 cut(s) 36, 143, 339, 534
DpnII GATC 4 cut(s) 34, 141, 337, 532
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 300, 442
EarI CTCTTC 2 cut(s) 300, 442
Eco88I CYCGRG 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 344
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 6 cut(s) 157, 272, 344, 363, 443, 539
FaqI GGGAC 1 cut(s) 294
FatI CATG 6 cut(s) 153, 268, 340, 359, 439, 535
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 1 cut(s) 544
FokI GGATG 2 cut(s) 182, 419
Fsp4HI GCNGC 1 cut(s) 544
GluI GCNGC 1 cut(s) 544
GsaI CCCAGC 1 cut(s) 550
HaeIII GGCC 3 cut(s) 158, 273, 414
HapII CCGG 1 cut(s) 167
HgaI GACGC 1 cut(s) 572
Hin1II CATG 6 cut(s) 157, 272, 344, 363, 443, 539
HinfI GANTC 6 cut(s) 356, 377, 388, 426, 450, 583
HpaII CCGG 1 cut(s) 167
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 368
Hpy188I TCNGA 6 cut(s) 138, 192, 230, 327, 468, 532
Hpy188III TCNNGA 4 cut(s) 392, 496, 536, 591
Hpy8I GTNNAC 1 cut(s) 368
HpyAV CCTTC 4 cut(s) 62, 143, 493, 527
HpyCH4V TGCA 4 cut(s) 153, 223, 304, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
Hsp92II CATG 6 cut(s) 157, 272, 344, 363, 443, 539
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 4 cut(s) 34, 141, 337, 532
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 1 cut(s) 530
LweI GCATC 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 457
MalI GATC 4 cut(s) 36, 143, 339, 534
MboI GATC 4 cut(s) 34, 141, 337, 532
MboII GAAGA 4 cut(s) 16, 287, 459, 485
MflI RGATCY 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 2 cut(s) 201, 305
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 4 cut(s) 350, 382, 420, 459
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 6 cut(s) 132, 178, 236, 284, 312, 565
Mox20I TGGCCA 1 cut(s) 158
MscI TGGCCA 1 cut(s) 158
MslI CAYNNNNRTG 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 2 cut(s) 167, 346
MvaI CCWGG 1 cut(s) 346
MwoI GCNNNNNNNGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 167
NdeII GATC 4 cut(s) 34, 141, 337, 532
NlaIII CATG 6 cut(s) 157, 272, 344, 363, 443, 539
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 457
PagI TCATGA 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 300
PfeI GAWTC 2 cut(s) 377, 583
PkrI GCNGC 1 cut(s) 545
PleI GAGTC 4 cut(s) 350, 382, 420, 458
PpsI GAGTC 4 cut(s) 350, 382, 420, 458
Psp6I CCWGG 1 cut(s) 344
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 1 cut(s) 189
PsuI RGATCY 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 168
SapI GCTCTTC 1 cut(s) 300
SatI GCNGC 1 cut(s) 544
Sau3AI GATC 4 cut(s) 34, 141, 337, 532
SchI GAGTC 4 cut(s) 350, 382, 420, 459
ScrFI CCNGG 2 cut(s) 167, 346
SetI ASST 7 cut(s) 73, 99, 228, 323, 375, 519, 545
SfaNI GCATC 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 168
Sse9I AATT 2 cut(s) 201, 305
StyD4I CCNGG 2 cut(s) 165, 344
TasI AATT 2 cut(s) 201, 305
TfiI GAWTC 2 cut(s) 377, 583
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 1 cut(s) 543
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 3 cut(s) 17, 486, 552
TspGWI ACGGA 4 cut(s) 244, 368, 576, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.